KR100899811B1 - Guided mode resonance filter including high refractive index organic material and optical biosensor having the same - Google Patents

Abstract

Disclosed are a resonance reflection light filter including an organic high refractive material and an optical biosensor having the same. The resonant reflected light filter according to the present invention includes an organic layer exposed on the upper surface opposite to the substrate in the diffraction grating formed on the substrate. The organic layer may be formed of a separate organic high refractive thin film that forms at least a portion of the diffraction grating or covers the top surface of the diffraction grating. The optical biosensor according to the present invention includes a resonance reflected light filter having an organic layer exposed on the top surface thereof. Antibodies that can specifically bind to the antigens contained in the sample to be tested are directly adsorbed or bound with high affinity to the organic layer of the resonance reflection filter.

High refractive index, organic layer, diffraction grating, nano dot, resonant reflected light filter, optical biosensor

Description

Resonance reflection filter including organic high refractive material and optical biosensor having the same {Guided mode resonance filter including high refractive index organic material and optical biosensor having the same}

FIG. 1 is a cross-sectional view showing the main components of a resonance reflected light filter according to a first embodiment of the present invention.

FIG. 2 is a cross-sectional view showing the main components of a resonance reflected light filter according to a second embodiment of the present invention.

3 is a cross-sectional view showing the main components of the resonance reflection light filter according to the third embodiment of the present invention.

4 is a cross-sectional view showing the main components of the resonant reflected light filter according to the fourth embodiment of the present invention.

FIG. 5 is a view schematically illustrating a main configuration of an optical biosensor according to a preferred embodiment of the present invention.

<Explanation of symbols for the main parts of the drawings>

100, 200, 300, 400: resonant reflected light filter, 110, 210, 310, 410: substrate, 120: high refractive organic diffraction grating, 220: organic diffraction grating, 222: organic diffraction thin film, 224: inorganic nano dots, 320: diffraction Lattice, 330: organic high refractive thin film, 420: diffraction grating, 430: high refractive thin film, 432: organic thin film, 434: inorganic nanodot, 500: optical biosensor, 510: resonant reflected light filter, 502: antibody, 512: light source, 514: reflected light detector, 516: transmitted light detector, 518: microlens, 600: antibody, 610: block, 700: fixed part.

The present invention relates to a resonant reflective light filter and an optical biosensor including the same, and more particularly, to a resonant optical filter and an optical biosensor including the same, which are used to measure a wavelength change of light depending on whether a biomaterial is reacted or modified.

Optical biosensors are widely used to detect biomaterials such as proteins, DNA, hormones, enzymes, and the like. The optical biosensor using a guided mode resonance filter among the optical biosensors uses the peak of the reflection spectrum generated by the diffraction grating which can serve as a high refractive index waveguide. When the light diffracted by the diffraction grating is coupled with the mode in which the light is guided through the high-refractive waveguide, the reflection spectrum appears to have a narrow line width, thereby realizing a highly sensitive biosensor.

The resonant reflective filter according to the prior art includes a nanometer diffraction grating formed by an electron beam lithography process or an imprint process that transfers a nano pattern using a stamp. In the resonant reflective filter manufacturing process according to the prior art example, in order to form a diffraction grating, first to form a high refractive layer made of an inorganic material having a larger refractive index than the substrate on a transparent substrate, and patterned by a dry or wet method, First, a nanometer pattern was formed on the surface of the substrate by dry or wet etching the substrate, and then a high refractive index layer formed of an inorganic material having a larger refractive index than the substrate was used. In a resonant reflective filter according to another example of the prior art, an organic nanopattern was formed on a substrate by using a stamp made of silicon or quartz to form a diffraction grating by an imprint process. However, the organic materials used to form the diffraction grating in the resonant reflection light filter according to the related art obtained by using the imprint process have a low refractive index, which does not satisfy the diffraction conditions for realizing a high sensitivity biosensor. Thus, a separate deposition process was involved to form a high refractive index inorganic thin film on the diffraction grating formed by the imprint process.

As described above, in the resonant reflection filter according to the prior art, a separate inorganic thin film formation process must be performed in order to secure a refractive index for satisfying a necessary diffraction condition. In addition, the inorganic thin film exposed on the surface of the diffraction grating has poor affinity or binding force with the biomaterial. Therefore, in order to adsorb or bind the biomaterial on the inorganic thin film, a separate surface modification process for introducing a specific reactor to the surface of the inorganic thin film is essential. Therefore, the resonant reflective filter according to the prior art has a complicated manufacturing process, takes a lot of process cost and time, and has a limitation in producing a stable biosensor.

SUMMARY OF THE INVENTION An object of the present invention is to solve the above-mentioned problems in the prior art, and provides a refractive index sufficient to obtain a desired diffraction condition and that a biomaterial capable of specifically binding to a test target material can be adsorbed or combined with excellent affinity. Therefore, when applied to the optical biosensor to provide a resonant reflection light filter that can accurately transfer the change in the resonant light wavelength according to the reaction or deformation of the biomaterial.

Another object of the present invention is to improve the detection sensitivity of the detection target material by employing a resonant reflecting light filter which is excellent in affinity with the biomaterial and can provide a sufficient refractive index to achieve the desired diffraction conditions and economically by a simple process. It is to provide an optical biosensor that can be manufactured.

In order to achieve the above object, the resonant reflection light filter according to the present invention is a substrate,

And a diffraction grating formed on the substrate, and an organic layer exposed on the upper surface of the opposite side of the substrate in the diffraction grating.

The organic layer may include at least a portion of the diffraction grating and may be formed of an organic material having a refractive index of 1.4 to 2.5. The substrate may be made of a semiconductor, glass, quartz, or a polymer film.

Alternatively, the organic layer may be formed of a separate organic high refractive thin film covering the top surface of the diffraction grating. In this case, the diffraction grating may be made of an organic material or an inorganic material. The organic high refractive thin film may be formed of an organic material having a refractive index of 1.4 to 2.5. Alternatively, the organic high refractive thin film may include an organic thin film made of an organic material and a plurality of inorganic nano dots dispersed in the organic thin film. The plurality of inorganic nanopoints may be formed of an oxide, a nitride, or a combination thereof.

In order to achieve the above another object, the optical biosensor according to the present invention is a resonant reflection light filter comprising a substrate, a diffraction grating formed on the substrate, and an organic layer exposed on the upper surface of the opposite side of the substrate in the diffraction grating It includes. In addition, the optical biosensor according to the present invention is specifically capable of binding to the antigen contained in the sample to be tested, the antibody directly adsorbed or coupled to the organic layer of the resonant reflecting light filter, and the resonant reflecting light filter A light source for irradiation, a reflected light detector for detecting the reflected light from the resonance reflection light filter, a transmitted light detector for detecting the light transmitted through the resonance reflection light filter from the light source, and light from the light source incident on the resonance reflected light filter And a microlens for reflecting the reflected light from the resonant reflected light filter to the reflected light detector.

The optical biosensor according to the present invention may further include a block formed in the organic layer of the resonant reflection light filter in order to prevent nonspecific reaction and binding of the antibody or antigen to the exposed surface of the organic layer.

In the resonant reflection light filter according to the present invention, the organic layer is exposed on the upper surface of the diffraction grating. Therefore, the biomaterial required by the optical biosensor can be adsorbed or combined with the organic layer with high affinity. The optical biosensor according to the present invention can be manufactured by a simple manufacturing process and a low manufacturing cost, and the biomaterial is coupled to the resonant reflection light filter with high affinity, thereby improving the process efficiency of the optical biosensor.

Next, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a cross-sectional view showing the main components of the resonance reflected light filter 100 according to the first embodiment of the present invention.

Referring to FIG. 1, the resonant reflection light filter 100 according to the first exemplary embodiment includes a substrate 110 and a high refractive organic material diffraction grating 120 formed on the substrate 110.

The high refractive organic material diffraction grating 120 may be formed of an organic material having a refractive index of about 1.4 to 2.5. In particular, the high refractive organic material diffraction grating 120 may be made of a material having an average transmittance of 50% or more in the wavelength band of 400 ~ 900 nm region. For example, the high refractive organic material diffraction grating 120 may be made of an acrylate or methacrylate-based polymer, or an acetylene-based polymer.

The substrate 110 may be made of a transparent substrate made of a semiconductor, glass, quartz, or a polymer film.

In order to form the high refractive organic material diffraction grating 120 on the substrate 110, an organic material may be formed on the surface of the substrate 110 using a wet process such as, for example, spin coating or dipping. The coating may form an organic thin film having a predetermined thickness, and then may be etched using photolithography or a recess may be formed using a nanoimprint process.

In the resonant reflection light filter 100, the resonance spectrum is formed while the light diffracted by the diffraction grating 120 guides the optical waveguide having a high refractive index. The diffraction period of the diffraction grating may be manufactured to be shorter than the average wavelength of the light source irradiated to the resonance reflected light filter 100.

When the organic material made of the high refractive organic material diffraction grating 120 is exposed on the upper surface of the resonance reflection light filter 100, the biomaterial is combined without the need for complicated surface treatment or a separate process such as vacuum deposition when the biomaterial is combined on the upper surface. This high affinity can be adsorbed or combined on the high refractive organic material diffraction grating 120. If necessary, the exposed surface of the high refractive organic material diffraction grating 120 may be subjected to an oxygen plasma treatment or a surface treatment using an acid solution such as sulfuric acid in order to facilitate the adsorption or bonding of the biomaterial.

2 is a cross-sectional view showing the main components of the resonance reflected light filter 200 according to the second embodiment of the present invention.

Referring to FIG. 2, the resonant reflection light filter 200 according to the second embodiment of the present invention includes a substrate 210 and an organic diffraction grating 220 formed on the substrate 210.

The organic diffraction grating 120 includes an organic diffraction thin film 222 and a plurality of inorganic nano dots 224 dispersed in the organic diffraction thin film 222.

The organic diffraction thin film 222 may be made of, for example, an acrylate or methacrylate-based polymer substituted or unsubstituted with a fluorine atom, or an acetylene-based polymer substituted or unsubstituted with a fluorine atom.

The plurality of inorganic nano dots 224 may each have a particle size of 1 to 200 nm. The plurality of inorganic nano dots 224 may each be formed of an oxide, a nitride, or a combination thereof. For example, the plurality of inorganic nanopoints 224 may be formed of SiO ₂ , TiO ₂ , InSnO, ZnO, SnO ₂ , NiO, Cu ₂ SrO ₂ , Si ₃ N ₄ , GaN, and In ₃ N ₂ , respectively. It may be made of any one or a combination thereof.

In order to form the organic diffraction grating 220 on the substrate 210, for example, an organic solution in which the inorganic nanodots 224 are dispersed using a wet process such as spin coating or dipping may be used. After coating on the surface to form an organic thin film in which the inorganic nano dot 224 having a predetermined thickness is dispersed, it may be etched using photolithography or a recess may be formed using a nanoimprint process.

Alternatively, the plurality of inorganic nano dots 224 may be formed of an inorganic element included in the organic compound constituting the organic diffraction thin film 222. In this case, in order to form the organic diffraction grating 220, for example, an organic compound containing an inorganic element is coated on the substrate 210 to form an organic thin film in which the inorganic element is dispersed, and then photolithography. The technique can be used to etch it or to form a recess using a nanoimprint process.

The organic diffraction grating 120 including the organic diffraction thin film 222 and a plurality of inorganic nano dots 224 dispersed in the organic diffraction thin film 222 in the resonance reflection light filter 200. By providing the organic layer composed of the organic diffraction thin film 222 is exposed on the upper surface. Thus, the biomaterial may be adsorbed or bonded onto the organic diffraction thin film 222 with high affinity. If necessary, the exposed surface of the organic diffraction grating 120 may be subjected to an oxygen plasma treatment or a surface treatment using an acid solution such as sulfuric acid in order to facilitate adsorption or bonding of the biomaterial.

The substrate 210 is as described with reference to the substrate 110 with reference to FIG.

3 is a cross-sectional view illustrating a main part of the resonance reflection light filter 300 according to the third embodiment of the present invention.

Referring to FIG. 3, the resonant reflection light filter 300 according to the third exemplary embodiment of the present invention includes a substrate 310, a diffraction grating 320 formed on the substrate 310, and the diffraction grating 320. It includes an organic high refractive thin film 330 formed on).

The diffraction grating 320 may be formed of an organic material or an inorganic material. For example, the diffraction grating 320 may be made of an organic material including an acrylate or methacrylate-based polymer substituted or unsubstituted with a fluorine atom, or an acetylene-based polymer not substituted or substituted with a fluorine atom. Alternatively, the diffraction grating 320 may be made of an inorganic material such as SiO ₂ , Si ₃ N ₄ , TiO _2, or the like.

In order to form the diffraction grating 320, a process similar to or similar to the manufacturing process of the high refractive organic material diffraction grating 120 as described with reference to FIG. 1 may be used.

The organic high refractive thin film 330 may be formed of an organic material having a refractive index of about 1.4 to 2.5. In particular, the organic high refractive thin film 330 may be formed of a material having an average transmittance of 50% or more in the wavelength band of 400 to 900 nm. For example, the organic high refractive thin film 330 may be made of an organic material including an acrylate or methacrylate-based polymer, or an acetylene-based polymer.

In order to form the organic high refractive thin film 330 on the diffraction grating 320, for example, a wet process such as spin coating or dipping may be used.

The organic high refractive thin film 330 is formed on the diffraction grating 320 in the resonance reflected light filter 300 to expose an organic layer formed of the organic high refractive thin film 330 on the upper surface. Therefore, the biomaterial may be adsorbed or combined on the organic high refractive thin film 330 with high affinity. If necessary, in order to further facilitate the adsorption or bonding of the biomaterial, the exposed surface of the organic high refractive thin film 330 may be subjected to an oxygen plasma treatment or a surface treatment using an acid solution such as sulfuric acid.

The substrate 310 is as described with reference to the substrate 110 with reference to FIG.

4 is a cross-sectional view illustrating a main part of the resonance reflection light filter 400 according to the fourth embodiment of the present invention.

Referring to FIG. 4, the resonant reflection light filter 400 according to the fourth embodiment of the present invention includes a substrate 410, a diffraction grating 420 formed on the substrate 410, and the diffraction grating 420. It includes a high refractive thin film 430 is formed on the).

The diffraction grating 420 may be made of an organic material or an inorganic material. For example, the diffraction grating 420 may be made of an organic material including an acrylate or methacrylate-based polymer substituted or unsubstituted with a fluorine atom, or an acetylene-based polymer substituted or not substituted with a fluorine atom. Alternatively, the diffraction grating 420 may be made of an inorganic material such as SiO ₂ , Si ₃ N ₄ , TiO _2, or the like.

In order to form the diffraction grating 420 on the substrate 410, for example, a process similar to or similar to that of manufacturing the high refractive organic material diffraction grating 120 as described with reference to FIG. 1 may be used.

The high refractive thin film 430 includes an organic thin film 432 and a plurality of inorganic nano dots 434 dispersed in the organic thin film 432.

The organic thin film 432 may be formed of, for example, an organic material including an acrylate or methacrylate-based polymer which is substituted or not substituted with a fluorine atom, or an acetylene-based polymer that is not substituted or substituted with a fluorine atom.

The plurality of inorganic nano dots 434 may each have a particle size of 1 to 200 nm. The plurality of inorganic nano dots 434 may be formed of an oxide, a nitride, or a combination thereof, respectively. For example, the plurality of inorganic nanopoints 434 are each composed of SiO ₂ , TiO ₂ , InSnO, ZnO, SnO ₂ , NiO, Cu ₂ SrO ₂ , Si ₃ N ₄ , GaN, and In ₃ N ₂ . It may be made of any one or a combination thereof.

In order to form the high refractive thin film 430 on the diffraction grating 420, an organic solution in which the inorganic nano dot 434 is dispersed using a wet process such as spin coating or dipping may be used. 420 may be used to coat the surface.

Alternatively, the plurality of inorganic nano dots 434 may be formed of an inorganic element included in an organic compound constituting the organic thin film 432. In this case, in order to form the high refractive thin film 430, for example, a method of coating an organic compound including an inorganic element on the diffraction grating 420 may be used.

Alternatively, in order to form the high refractive thin film 430, an organic compound including an inorganic element may be immobilized on a surface of the diffraction grating 420 by a self-assembly process.

The organic thin film 432 is provided by the high refractive thin film 430 including the organic thin film 432 and the plurality of inorganic nano dots 434 dispersed in the organic thin film 432 in the resonance reflection light filter 400. The organic layer which consists of is exposed to an upper surface. Therefore, the biomaterial may be adsorbed or combined on the organic thin film 432 with high affinity. If necessary, in order to further facilitate the adsorption or bonding of the biomaterial, the exposed surface of the high refractive thin film 430 may be subjected to an oxygen plasma treatment or a surface treatment using an acid solution such as sulfuric acid.

The substrate 410 is the same as the substrate 110 described with reference to FIG. 1.

The resonant reflection light filter 100, 200, 300, or 400 according to the present invention described above is a high refractive thin film 330 formed on the diffraction grating 120, 220, 320, 420 or the diffraction grating 320, 420 causing resonance. , 430 is each composed of an organic material, or is composed of a mixed material based on an organic material and including inorganic nanopoints. Therefore, when applying the resonant reflection light filter (100, 200, 300, 400) according to the present invention to the optical biosensor, the affinity with the biomaterial that can be specifically coupled to the detection target material is improved to improve the efficiency of the detection process You can.

FIG. 5 is a diagram schematically illustrating a main configuration of an optical biosensor 500 according to an exemplary embodiment of the present invention.

Referring to FIG. 5, an optical biosensor 500 according to a preferred embodiment of the present invention includes a resonance reflected light filter 510.

The resonance reflected light filter 510 may have a structure of any one of the resonance reflected light filters 100, 200, 300, and 400 described with reference to FIGS. 1 to 4. 5 illustrates a case in which the resonance reflection light filter 510 has the same structure as the resonance reflection light filter 400 illustrated in FIG. 4. However, the present invention is not limited thereto, and the resonance reflected light filter 510 having various structures within the scope of the inventive concept may be used. For a detailed description of the resonance reflection light filter 510, the resonance reflection light filter 100, 200, 300, and 400 will be described with reference to FIGS. 1 to 4, and a detailed description thereof will be omitted.

In the optical biosensor 500, an antibody 502 that can specifically bind to the antigen 600 included in a sample (not shown) to be examined is directly adsorbed or bound to the organic layer of the resonance reflection light filter 510. It is. In addition, a block 610 formed in the organic layer of the resonant reflection light filter 510 may be further included to prevent nonspecific reaction and binding of the antibody 502 or the antigen 600 to the exposed surface of the organic layer. have.

In FIG. 5, a case in which the organic layer of the resonance reflection light filter 510 is formed of the high refractive thin film 430 formed on the diffraction grating 420 is illustrated. However, the present invention is not limited to this and various modifications and changes are possible. For example, the organic layer of the resonance reflection light filter 510 may be composed of the high refractive organic material diffraction grating 120 illustrated in FIG. 1. Alternatively, the organic layer of the resonance reflection light filter 510 may be composed of the organic diffraction grating 220 illustrated in FIG. 2. Alternatively, the organic layer of the resonance reflection light filter 510 may be composed of the organic high refractive thin film 330 illustrated in FIG. 3.

In addition, the optical biosensor 500 may include a light source 512 for irradiating light to the resonance reflection light filter 510, a reflection light detector 514 for detecting the reflection light from the resonance reflection light filter 510, and And a transmitted light detector 516 for detecting the light transmitted from the light source 512 through the resonance reflected light filter 510. The light source 512 may be a white light source or a laser light source having a specific wavelength. In order to inject light from the light source 512 into the resonant reflection light filter 510, and to reflect the reflected light from the resonant reflection light filter 510 to the reflection light detector 514 and the resonant reflection light filter 510 A microlens 518 is provided between the reflected light detector 514.

Referring to the operating principle of the optical biosensor 500 according to the preferred embodiment of the present invention illustrated in FIG.

First, the block 610 for preventing nonspecific reaction and binding between the antibody 502 and a specific biomaterial is adsorbed or combined on the high refractive thin film 430 formed on the diffraction grating 420 of the resonance reflection light filter 510. The test sample is applied to the resonant reflection light filter 510 in the presence state. When an antibody 600 capable of specifically binding to the antigen 502 is present in the test sample, the antibody 600 is specifically bound to the antigen 502. As the antigen 502 and the antibody 600 bind as described above, the optical refractive index of the resonance reflected light filter 510 is changed. In order to detect the resonant wavelength change in the resonant reflection light filter 510 according to the refractive index change, the manner in which light passes through the resonant reflection light filter 510, or the light is reflected from the resonant reflection light filter 510. The transmitted light detector 516 or the reflected light detector 514. That is, the light emitted from the light source 512 fixed on the fixing part 700 passes through the resonant reflection light filter 510 through the microlens 518 or is reflected by the resonant reflection light filter 510. Light transmitted or reflected in this manner is detected by the transmitted light detector 516 or the reflected light detector 514. The transmitted light detector 516 and the reflected light detector 514 may be configured as a photodetector or a spectrometer for measuring a spectrum.

Optical biosensor 500 according to a preferred embodiment of the present invention having the configuration described above is a protein, on the high refractive thin film 430 formed on the organic layer, for example, the diffraction grating 420 of the resonant reflection light filter 510 Adsorption or binding of biomaterials such as DNA, hormones, enzymes, etc., has a function as a biosensor.

If necessary, the organic layer may be more easily adsorbed or bonded to the surface of the organic layer to which the biomaterial is adsorbed or bonded, for example, to the surface of the high refractive thin film 430 formed on the diffraction grating 420. The surface of can be oxygen plasma treated. In order to perform oxygen plasma treatment on the surface of the organic layer, plasma treatment may be performed while applying RF power of less than 100 W using, for example, O ₂ , Ar, and N ₂ gases. Alternatively, by treating the organic layer with an acidic or alkaline solution, a reactor such as an -OH group may be formed on the surface of the organic layer. As a treatment liquid for forming a reactor on the surface of the organic layer, for example, an acidic solution such as H ₂ SO ₄ , HF, HNO ₃ , HCl, H ₃ PO ₄ , or NaOH, KOH, Ca (OH) ₂ , NH Alkaline solutions such as ₄ OH can be used.

As described above, a preferred embodiment of the optical biosensor 500 according to the present invention has been described, but the present invention is not limited thereto, and various modifications within the scope of the claims and the detailed description of the invention and the accompanying drawings are provided. It is possible. For example, in the above embodiments, the diffraction grating of the resonant reflection light filter or the high refractive thin film formed thereon is illustrated in various embodiments in which the organic layer is formed, but the present invention is limited to the illustrated configuration. The organic layer is exposed on the opposite side of the substrate in the diffraction grating, that is, the organic layer is composed of an organic layer on which a surface capable of adsorbing or binding an antibody specifically capable of binding to an antigen included in a sample to be tested. As far as possible, various modifications and changes are possible by combining various configurations that may be implemented within the scope of the present invention.

In the resonance reflection light filter according to the present invention, an organic layer composed of an organic material having a high refractive index, or an organic layer composed of an organic thin film in which a plurality of inorganic nanopoints having a high refractive index is dispersed is exposed on an upper surface of a diffraction grating. Therefore, the biomaterial required by the optical biosensor can be adsorbed or combined with the organic layer with high affinity.

In the optical biosensor according to the present invention, the manufacturing process can be simplified, thereby lowering the manufacturing cost, and the biomaterial is coupled to the resonant reflection light filter with high affinity, thereby improving the process efficiency of the optical biosensor.

In the above, the present invention has been described in detail with reference to preferred embodiments, but the present invention is not limited to the above embodiments, and various modifications and changes by those skilled in the art within the spirit and scope of the present invention. This is possible.

Claims (16)

Substrate, A diffraction grating formed on said substrate and made of a material different from said substrate, The diffraction grating includes an organic diffraction thin film made of an organic material, and a plurality of inorganic nanodots dispersed in the organic diffraction thin film. The method of claim 1, The plurality of inorganic nano-dots are made of oxide, nitride, or a combination thereof. The method of claim 1, The organic diffraction thin film is a resonant reflection light filter, characterized in that consisting of acrylate or methacrylate-based polymer (meth) acrylate based polymer, or acetylene-based polymer (acetylene based polymer). The method of claim 1, The substrate is a resonant reflection light filter, characterized in that consisting of a semiconductor, glass, quartz, or a polymer film. Substrate, A diffraction grating formed on the substrate, An organic layer exposed on an opposite upper surface of the substrate in the diffraction grating, The organic layer is a resonant reflection light filter, characterized in that consisting of a separate organic high refractive thin film covering the upper surface of the diffraction grating. The method of claim 5, The diffraction grating is a resonance reflected light filter, characterized in that the organic material. The method of claim 5, The diffraction grating is a resonance reflected light filter, characterized in that the inorganic material. The method of claim 5, The organic high refractive thin film is a resonance reflection light filter, characterized in that the organic material having a refractive index of 1.4 to 2.5. The method of claim 5, The organic high refractive thin film comprises an organic thin film made of an organic material and a plurality of inorganic nano-dots (nano-dot) dispersed in the organic thin film resonant reflection light filter, characterized in that. The method of claim 9, The plurality of inorganic nano-dots each has a particle size of 1 ~ 200 nm resonant reflection light filter, characterized in that. The method of claim 9, The plurality of inorganic nano-dots are made of oxide, nitride, or a combination thereof. The method of claim 9, The plurality of inorganic nanopoints are any one selected from the group consisting of SiO ₂ , TiO ₂ , InSnO, ZnO, SnO ₂ , NiO, Cu ₂ SrO ₂ , Si ₃ N ₄ , GaN, and In ₃ N ₂ , or a combination thereof. Resonant reflected light filter, characterized in that consisting of. A resonance comprising a substrate, a diffraction grating formed on the substrate, and an organic layer exposed on the opposite side of the substrate in the diffraction grating, the organic layer being a separate organic high refractive thin film covering the top surface of the diffraction grating. Reflected light filter, An antibody capable of specifically binding to the antigen contained in the sample to be tested and being directly adsorbed or bound to the organic layer of the resonant reflected light filter; A light source for irradiating light to the resonance reflected light filter; A reflected light detector for detecting reflected light from the resonance reflected light filter; A transmission light detector for detecting light transmitted from the light source through the resonance reflection light filter; And a microlens for injecting light from the light source into the resonance reflection light filter and reflecting the reflection light from the resonance reflection light filter to the reflection light detector. The method of claim 13, And a block formed in the organic layer of the resonant reflecting light filter to prevent nonspecific reaction and binding of the antibody or antigen to the exposed surface of the organic layer. The method of claim 13, The diffraction grating is an optical biosensor, characterized in that the organic material having a refractive index of 1.4 to 2.5. The method of claim 13, The diffraction grating is an optical biosensor, characterized in that the inorganic material.

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