CN103018172B - Polymer grating aptamer biosensor and detecting device - Google Patents
Polymer grating aptamer biosensor and detecting device Download PDFInfo
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- CN103018172B CN103018172B CN201210528258.XA CN201210528258A CN103018172B CN 103018172 B CN103018172 B CN 103018172B CN 201210528258 A CN201210528258 A CN 201210528258A CN 103018172 B CN103018172 B CN 103018172B
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Abstract
The invention discloses a polymer grating aptamer biosensor and a detecting device. The biosensor comprises a base body; an optical plane is formed on the lower surface of the base body; a polymer concave convex grating is formed on the upper surface of the base body; the polymer concave convex grating comprises a plurality of parallel grooves arranged at equal intervals; and a layer of aptamer is deposited in the groove. The detecting device comprises a metal sample box, a semiconductor laser, a beam splitter, a cylindrical lens, an optical attenuator, a linear array CCD (Charge Coupled Device) sensor, a data processing module and the biosensor, wherein the biosensor is fixed on the metal sample box; centers of the beam splitter, the cylindrical lens, the optical attenuator and the linear array CCD (Charge Coupled Device) sensor are positioned in an identical optical axis; the semiconductor laser comprises a red light semiconductor laser and a green light semiconductor laser; and the red light semiconductor laser and the green light semiconductor laser form a 90-degree angle and are installed at the bottom and on the right side of the beam splitter. The detecting device realizes complete non contact measurement, and does not damage samples to be measured.
Description
Technical field
The invention belongs to detection and field of sensing technologies, be specifically related to a kind of aptamer biology sensor and pick-up unit.
Background technology
In recent years, biology sensor is in the ascendant at the research and apply of scientific research, biomedicine and industrial circle, such as medical diagnosis, Row control and environmental monitoring etc.Biology sensor is using biology assembly as main functional elements, can experience specifically measured and convert thereof into device or the device of discernible signal according to certain rule.
Invention high speed, high specific, high-sensitive protein detection techniques are the urgent tasks that current biology sensor research institute faces.The detection of traditional protein mainly utilizes the special interaction of antibody-antigene, and the appearance of and the artificial synthetic oligonucleotide utilizing identification strict between oligonucleotides and affinity and design-fit, makes antibody antigen react and new revolutionary variation occurs.The aptamer screened can the various part such as conjugated protein, polypeptide, organism, metallic ion specifically, and aptamer has easy synthesis, easily stores, the easy advantage such as modifications, its research in the diversity, protein/DNA interaction etc. of nucleic acid structure comes into one's own day by day.
The immobilization technology of biologically active unit is the core that biology sensor makes, and the immobilization technology of existing biology sensor mainly contains absorption method, covalent bonding method, physically trapping method etc.Absorption method is generally undertaken by the volatilization containing enzyme buffer liquid, but adsorption process has reversibility, and biologically active unit easily departs from from electrode surface, and the life-span is shorter; Covalent bonding method is combined with electrode surface by covalent bond by biologically active unit and fixes, and enzyme stability is better, but the covalent bonding of electrode surface is more difficult.Physically trapping method adopts the embedding of gel, polymkeric substance, and biomolecule embedded and be fixed in the space net structure of high molecular polymer, good stability, process is simple.But adopt physical method that gel/polymkeric substance is limited in electrode surface, make sensor be difficult to microminiaturization.The biologically active unit fixation support of current use, Method and Technology do not reach perfect degree, one of therefore simpler, practical novel immobilization technology important directions remaining this area research.
Existing aptamer biology sensor mainly contains optics aptamer biosensors, piezoelectric crystal aptamer sensor and galvanochemistry aptamer sensor.Existing optics aptamer biosensors has optical fiber aptamer sensor, evanescent wave optical fiber aptamer sensor, surface plasma resonance aptamer sensor and fluorescence aptamer sensor.
The basic sensors of existing fiber aptamer biosensors is silica fibre, and the mechanism of action utilizes quartz surfaces first to connect one deck connector, is then connected on fiber end face by fit, carries out biochemical reaction with object.The characteristic optical signal (fluorescence, color etc.) produced after reaction is passed to photodetector by fibre-optical probe, through opto-electronic conversion and then mensuration object content.
Existing evanescent wave optical fiber aptamer sensor adopts and adds that bio-sensitive film (aptamer) realizes the measurement to object on optical fiber side surface.Principle is when light is propagated in total reflection mode in fiber core, and can there is a light wave fields in other medium outside fibre core side surface, this ripple is rapid decay with outside propagation distance, is called evanescent wave.When evanescent wave is through bio-sensitive film, or generation light signal, or cause propagating in evanescent wave and optical fiber the intensity of light, phase place or frequency shift, measure these and change, the information that bio-sensitive film changes can be obtained, namely fit with analysis thing interactional information.
The shortcoming of above-mentioned optical fiber aptamer biosensors and evanescent wave optical fiber aptamer sensor is the fixing difficulty of biologically active unit, and basic sensors can not be reused and change inconvenience.
Existing surface plasma resonance aptamer sensor is solidificated in by aptamer molecule with quartz or glass metal (being generally gold) the film surface that is carrier, add object to be measured, both combinations make the refractive index of metal film and solution interface rise, thus cause the change of resonance angle.If be fixed into shooting angle, just can carry out quantitative test according to the change degree of resonance angle to object to be measured, the method is without the need to mark, very convenient.But due to needs noble metal film, sensor cost is higher.
Existing fluorescence aptamer biosensors mainly uses fluorophor labeling nucleic acid fit, the fluorescence polarization produced after based target molecule and aptamer effect or the change of fluorescence intensity are to detect target molecule, or fluorophor and quencher being marked on aptamer respectively, realizing the quantitative test to determinand by target molecule being incorporated into the change of fluorescence signal after in system.Fluorescence aptamer biosensors structure is simple, but accuracy in detection is relatively low.
Optic polymer has easy processing, lightweight and be suitable for the advantages such as batch production, and therefore polymer grating is widely used in the fields such as optical measurement, integrated optics, optical information processing.The Microstructure Grating adopting microelectronic technique to make is an important directions of novel grating development, and its minimum feature can accomplish to be less than 100 nanometers.Carry out the research of various reproduction technology for this reason both at home and abroad, mainly comprise hot pressing, injection moulding, casting and sol-gel process, stamped method etc.Utilize these methods to carry out copying of Microstructure Grating, use same master grating to can be mass-produced out the identical replica grating of grating parameter, cost of reproduction is low.
No matter which kind of scientific instrument, its development trend is always towards miniaturization, intellectuality, high precision and low price future development.Biology sensor kind is a lot, new method constantly substitutes classic method, therefore the domestic and international research work in this field is from uninterruptedly, research can be used for immune detection and detection of nucleic acids and as the low cost of bioprocesses various in other life science, convenient, fast biomolecule detecting method, for hospital, bio-pharmaceuticals department, quarantine and examination department and life science unit, all significant especially for family's immunity, health monitoring etc., application prospect is good.
Summary of the invention
In view of this, the present invention is directed to the disappearance of prior art existence, its fundamental purpose is to provide a kind of polymer grating aptamer biology sensor and pick-up unit, equipment is simple, detection is easy, good stability, process are simple, cost of manufacture is low, realize Entirely contactless measures, to tested sample not damaged, thus overcome the deficiencies in the prior art.
For achieving the above object, the present invention adopts following technical scheme:
A kind of polymer grating aptamer biology sensor, comprise the matrix of a slice optics plastic production, the lower surface of this matrix is optical flat, upper surface is the concavo-convex grating of polymkeric substance, the concavo-convex grating of this polymkeric substance is made up of the parallel groove of many spaced sets, and deposit one deck aptamer in groove.
Preferably, described matrix is rectangular-shaped, and its length is 5 millimeters, and width is 3 millimeters, is highly 1 millimeter, and the grating constant of the concavo-convex grating of described polymkeric substance is 1 to 2 micron, and the grating groove degree of depth is 1 to 2 micron, and the deposit thickness of aptamer is 10-100 nanometer.
Preferably, described matrix is polycarbonate or polymethyl methacrylate materials.
A kind of polymer grating aptamer biological monitor, comprise metal sample box, semiconductor laser, spectroscope, cylindrical lens, optical attenuator, linear CCD sensor, data processing module and aforesaid biology sensor, this biology sensor is fixed on metal sample box, this optical attenuator and linear CCD sensor are positioned at the top of biology sensor successively, this data processing module is connected with linear CCD sensor, this cylindrical lens and spectroscope are positioned at the below of biology sensor successively, described semiconductor laser comprises red light semiconductor laser and green light semiconductor, this red light semiconductor laser and green light semiconductor are mutually an angle of 90 degrees and are arranged on spectroscopical bottom and right side, this spectroscope, cylindrical lens, optical attenuator, linear CCD sensor center is on same optical axis.
Preferably, the center of described metal sample box is provided with a rectangular depression region, the length in this rectangular depression region is 5 millimeters, width is 3 millimeters, be highly 1 millimeter, and the centre bit in rectangular depression region is equipped with a circle leads to light aperture, and the upper surface four direction of metal sample box respectively there is a groove-shaped notch being used as to place and take out biology sensor, the width of each groove-shaped notch is 1.5 millimeters, and the degree of depth is 1 millimeter.
Preferably, the power of described red light semiconductor laser is 3 ~ 5 milliwatts, wavelength is 650 nanometers, output beam diameter is 1 ~ 2 millimeter, the power of described green light semiconductor is 5 ~ 10 milliwatts, wavelength is 532 nanometers, output beam diameter is 1 ~ 2 millimeter, the exiting surface of this ruddiness, green light semiconductor is apart from spectroscopical surperficial 10 ~ 20 millimeters, and the central axis of ruddiness, green light semiconductor and spectroscopical two mutually perpendicular deads in line.
Preferably, described spectroscope is cube glass Amici prism, and the length of side is 10 ~ 15 millimeters; Described cylindrical lens is silica cylindrical lenses that is long 10 millimeters, wide 5 millimeters, focal length 10 millimeters, this cylindrical lens be arranged on spectroscopical directly over 5 millimeters of places; Described metal sample box is positioned over 10 millimeters of places directly over cylindrical lens, the center superposition of the focus of cylindrical lens and the logical light aperture of the circle of metal sample box.
Preferably, described optical attenuator is positioned over 30 millimeters, the top place of metal sample box, this optical attenuator is made up of two film polarizer, the diameter of two film polarizer is all 30 millimeters, one of them film polarizer maintains static, and another film polarizer can rotate freely to change laser light damping capacity around central axis.
Preferably, described linear CCD sensor is positioned over 10 millimeters, the top place of optical attenuator, and the CCD length of this linear CCD sensor is not less than 25 millimeters, and photosensitive element is 2700, and photosensitive element is of a size of 11 × 11 microns, spectral response range 0.3 ~ 0.9 micron; Described optical attenuator and linear CCD sensor are coaxially arranged in the same box that can move left and right, and when measuring the diffracted light intensity of biology sensor, box is positioned at directly over metal sample box; When placing and take out biology sensor, box is positioned at the upper left side of metal sample box.
Preferably, described data processing module comprises 12 A/D converters and single-chip microcomputer.
The present invention compared with prior art has obvious advantage and beneficial effect, specifically, as shown from the above technical solution: (1) adopts micro-structural polymer concavo-convex grating embedding biomolecule, and good stability, process is simple, belongs to novel immobilization technology.(2) adopt diffraction intensity ratio measuring method, detect easily, equipment is simple.(3) adopt the concavo-convex grating of micro-structural polymer as biology sensor, cost of manufacture is low.(4) Entirely contactless is measured, to tested sample not damaged.
For more clearly setting forth architectural feature of the present invention and effect, below in conjunction with accompanying drawing and specific embodiment, the present invention is described in detail.
Accompanying drawing explanation
Fig. 1 is the front view of aptamer biosensor structure of the present invention.
Fig. 2 is the vertical view of Fig. 1.
Fig. 3 is structure of the detecting device schematic diagram of the present invention.
Fig. 4 is detection light path principle figure of the present invention.
Fig. 5 is the left view of Fig. 4.
Fig. 6 is metal sample box structural representation.
Fig. 7 is CCD detection face hot spot distribution schematic diagram.
Accompanying drawing identifier declaration:
1, aptamer biology sensor 11, the concavo-convex grating of polymkeric substance
12, aptamer 13, optical flat
2, metal sample box 21, circular logical light aperture
22, rectangular depression region 23, groove-shaped notch
3, semiconductor laser 31, red light semiconductor laser
32, green light semiconductor 4, spectroscope
5, cylindrical lens 6, optical attenuator
7, linear CCD sensor 8, data processing module
9, box.
Embodiment
Please refer to shown in Fig. 1 and Fig. 6, polymer grating aptamer optical biosensor 1 of the present invention is the matrix of the rectangular shape that a piece is made by optical plastic thin slice, be provided with the concavo-convex grating 11 of polymkeric substance in body upper surface, the bottom surface of matrix is optical flat 13.Described matrix material is polycarbonate, is commonly called as PC, also can adopt polymethylmethacrylate, be commonly called as PMMA, and the latter's performance is slightly poor.The making of the concavo-convex grating of described polymkeric substance 11 can adopt hot press printing technology by the concavo-convex grating replication on optical grating mold plate on polymer matrix film.The concavo-convex grating 11 of this polymkeric substance is that the parallel groove being rectangle by one group of cross section is formed, and the direction of parallel groove is parallel with the long limit of rectangular base or minor face.In the present embodiment, the length of this matrix is 5 millimeters, and width is 3 millimeters, is highly 1 millimeter, and the grating constant of the concavo-convex grating 11 of polymkeric substance is 1 to 2 micron, and grating groove depth is 1 to 2 micron.In the groove of the concavo-convex grating 11 of polymkeric substance, deposited one deck aptamer 12, this aptamer 12 is DNA or the RNA oligonucleotide of efficient with part, single-minded combination, aptamer 12 thickness 10-100 nanometer, deposits to aptamer 12 in the groove of the concavo-convex grating 11 of polymkeric substance by volatilization deposition and centrifugation technique.
Please refer to Fig. 3, polymer grating aptamer biological monitor of the present invention comprises metal sample box 2, semiconductor laser 3, spectroscope 4, cylindrical lens 5, optical attenuator 6, linear CCD sensor 7, data processing module 8 and aforementioned optical biology sensor 1.This optical biosensor 1 is fixed on metal sample box 2, this optical attenuator 6 and linear CCD sensor 7 are positioned at directly over optical biosensor 1 successively, this data processing module 8 is connected with linear CCD sensor 7, this cylindrical lens 5 and spectroscope 4 are positioned at immediately below optical biosensor 1 successively, described semiconductor laser 3 comprises red light semiconductor laser 31 and green light semiconductor 32, this red light semiconductor laser 31 and green light semiconductor 32 are mutually bottom and the right side that an angle of 90 degrees is arranged on spectroscope 4, this spectroscope 4, cylindrical lens 5, optical attenuator 6, linear CCD sensor 7 center is on same optical axis.
Specifically, please refer to Fig. 6, described metal sample box 2 is the rectangular structure of " recessed " shape in, and bottom center position has a circle to lead to light aperture 21, as the logical light aperture that optical grating diffraction is measured.The length of the central rectangular sunk area 22 of this metal sample box 2 is 5 millimeters, and width is 3 millimeters, is highly 1 millimeter, and the size in this rectangular depression region 22 and the size of optical biosensor 1 match, for placing optical biosensor 1.Respectively there is a groove-shaped notch 23 on upper surface four limits of described metal sample box 2, and convenient placement and the concavo-convex grating 11 of taking-up polymkeric substance, the width of groove-shaped notch 23 is 1.5 millimeters, and the degree of depth is 1 millimeter.The effect of metal sample box 2 is fixing and protection optical biosensor 1, when placing optical biosensor 1, the optical flat 13 of concavo-convex for polymkeric substance grating 11 should be close to metal sample box 2 leads to light aperture 21 bottom surface with circle.
Please refer to Fig. 3, 4, 5, the power of described red light semiconductor laser 31 is 3 ~ 5 milliwatts, wavelength 650 nanometer, output beam diameter is 1 ~ 2 millimeter, the power of described green light semiconductor 32 is 5 ~ 10 milliwatts, wavelength 532 nanometer, output beam diameter is 1 ~ 2 millimeter, this ruddiness, green light semiconductor 31, 32 are mutually bottom and the right side that an angle of 90 degrees is arranged on spectroscope 4, and ruddiness, green light semiconductor 31, 32 exiting surface distance spectroscope 4 10 ~ 20 millimeters, surface, ruddiness, green light semiconductor 31, the central axis of 32 and two mutually perpendicular deads in line of spectroscope 4.
Described spectroscope 4 is cube glass Amici prism, the length of side is 10 ~ 15 millimeters, above-mentioned semiconductor laser 3 output beam is incorporated in the same light path upwards transmitted by spectroscope 4, the mode that takes turns to operate is adopted, timesharing Output of laser when described red light semiconductor laser 31 and green light semiconductor 32 are measured.
Described cylindrical lens 5 is silica cylindrical lenses that is long 10 millimeters, wide 5 millimeters, focal length 10 millimeters, and this cylindrical lens 5 is arranged on 5 millimeters of places directly over above-mentioned spectroscope 4, and laser beam passes through the center of cylindrical lens 5.
Directly over cylindrical lens 5, metal sample box 2 is placed at 10 millimeters of places, and laser beam is by the logical light aperture 21 of bottom surface circle of metal sample box 2.Place optical biosensor 1 in the rectangular depression region 22 of metal sample box 2, laser beam is by the center of optical biosensor 1.
Optical attenuator 6 is placed at 30 millimeters, the top place of metal sample box 2.Optical attenuator 6 is made up of two film polarizer, changes attenuation coefficient by the relative optical axis angle rotating two optical polarizers.The diameter of two film polarizer is all 30 millimeters, and one of them film polarizer maintains static, and another film polarizer can rotate freely around central axis, to change laser light damping capacity.
Linear CCD sensor 7 is placed at 10 millimeters, the top place of optical attenuator 6, the photosensitive element of linear CCD sensor 7 is 2700, photosensitive element size is not more than 11 × 11 microns, spectral response range 0.3 ~ 0.9 micron, CCD length is not less than 25 millimeters, and CCD length direction is parallel to above-mentioned cylindrical lens 5 cylinder axis direction.This optical attenuator 6 and above-mentioned linear CCD sensor 7 are coaxially arranged in same box 9, and box 9 can move left and right.When measuring the diffracted light intensity of optical biosensor 1, box 9 is positioned at directly over metal sample box 2; When placing and take out optical biosensor 1, box 9 is positioned at the upper left side of metal sample box 2.
What be connected with linear CCD sensor 7 is data processing module 8, primarily of 12 A/D converters and single-chip microcomputer composition, completes the collection of light signal strength, storage and data and calculates.
Please refer to Fig. 3, principle of work of the present invention is as follows: redness (the wavelength 650 nanometer) laser beam that red light semiconductor laser 31 sends, cylindrical lens 5 is entered without turnover through spectroscope 4, green (the wavelength 532 nanometer) laser beam that green light semiconductor 32 sends, transfers after 90 degree through spectroscope 4 and enters cylindrical lens 5.As shown in Figures 4 to 7, see in YZ plane, it is constant that laser beam still retains original width of light beam after cylindrical lens 5, and after leading to light aperture through the lower surface of metal sample box 2, beam width becomes identical with hole diameter, then the optical grating diffraction on optical biosensor 1, produces ± 1 grade and 0 order diffraction light, resolve into the light pencil of three different angles, eventually pass optical attenuator 6 and arrive linear CCD sensor 7 test surface.See in XZ plane, laser beam converges on the logical light aperture 21 of lower surface circle of metal sample box 2 by cylindrical lens 5, become fan-shaped laser beam after leading to light aperture 21 through circle and enter optical biosensor 1, because laser beam fan-shaped plan is parallel with the grating orientation on optical biosensor 1, so fan-shaped laser beam is constant through still retaining original fan shape after optical biosensor 1.Above-mentioned fan-shaped laser beam arrives linear CCD sensor 7 test surface after optical attenuator 6, on test surface, (XY plane) forms strip hot spot, although therefore linear CCD sensor 7 photosensitive element size only has 11 × 11 microns, the fan-shaped laser beam that the present invention adopts but is easy to be detected by linear CCD sensor 7, there is not beam alignment problem (please refer to Fig. 7).Linear CCD sensor 7 detects ± 1 grade and 0 order diffraction light intensity voltage signal, is stored in the storer of Single Chip Microcomputer (SCM) system after A/D conversion, then calculates the diffracted light intensity ratio of grating ± 1 grade and 0 grade to determine polymkeric substance concavo-convex grating 11 depth of groove.
Measuring process is as follows: adopt volatilization deposition and centrifugation technique to be deposited to by aptamer 12 in the groove of the concavo-convex grating 11 of polymkeric substance, use respectively the red laser of wavelength 650 nanometer (being sent by red light semiconductor laser 31) and wavelength 532 nanometer green laser (being sent by green light semiconductor 32) measure optical biosensor 1 ± 1 grade and 0 order diffraction light intensity, the grating groove degree of depth is determined by the diffracted light intensity ratio calculating grating ± 1 grade and 0 grade, the reason of two optical maser wavelengths is adopted to be multiple grating groove depth values of diffracted light intensity ratio correspondence of grating ± 1 determined grade and 0 grade, two wavelength are adopted just can uniquely to determine the grating groove degree of depth within the scope of certain depth.Tested protein is placed in the grating surface of optical biosensor 1, grating surface is cleaned after question response certain hour, repeat said process again and measure the grating groove degree of depth, by comparing the variable quantity of the grating groove degree of depth on optical biosensor 1, existence and the content of tested protein just can be obtained.
In sum, design focal point of the present invention is, the matrix of the optical biosensor of described aptamer has the concavo-convex grating 11 of polymkeric substance, by the mode fixing biological molecules that the physisorption of gentleness combines with embedding, namely by the effect of aptamer 12 molecular polarity key, hydrogen bond, hydrophobic bond, by volatilization deposition and centrifugation technique, aptamer 12 is adsorbed in the groove of the concavo-convex grating 11 of polymkeric substance, because recess width only has hundreds of nanometer to several microns, thus groove two wall has the effect of embedding fixing biological molecules.Aptamer 12 in the groove of the concavo-convex grating 11 of polymkeric substance combines with specific target molecules, and its specificity, as synantibody, has the affinity of strict recognition capability and height, thus makes the grating groove degree of depth change to combinative part.The Single wavelength laser beam that above-mentioned semiconductor laser 3 exports converges on the logical light aperture of metal sample box 2 circle through cylindrical lens 5 in the vertical direction, fan beam shape is become in the direction perpendicular to cylindrical lens 5 cylinder axis like this from the light beam of logical light aperture outgoing, still keeping parallelism beam shape on the direction being parallel to cylindrical lens 5 cylinder axis, just width of light beam narrows, and is conducive to improving spatial resolution.Fan-shaped laser beam forms multiple diffraction fan beam after the optical grating diffraction with aptamer 12 and part, arrives CCD photosurface after optical attenuator 6.Because the depth of groove of the comparison grating of concavo-convex grating ± 1 grade and 0 order diffraction light intensity is responsive, the change of depth of groove therefore just can be obtained by comparing grating ± 1 grade and 0 order diffraction beam intensity ratio.When there is the association reaction of aptamer 12 and part in grating groove, depth of groove will change.Receive 0 order diffraction and ± 1 order diffraction light by the CCD photosurface in this pick-up unit, compared the variable quantity of grating ± 1 grade and 0 order diffraction beam intensity ratio by data processing module 8, just can obtain the information of part biomolecule existence.
The present invention relative to conventional art and also have equipment simple, detect easily, good stability, process are simple, cost of manufacture is low, realize Entirely contactless measures, to the undamaged advantage of tested sample.
The above, it is only preferred embodiment of the present invention, not technical scope of the present invention is imposed any restrictions, thus every above embodiment is done according to technical spirit of the present invention any trickle amendment, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (7)
1. a polymer grating aptamer biological monitor, it is characterized in that: comprise metal sample box, semiconductor laser, spectroscope, cylindrical lens, optical attenuator, linear CCD sensor, data processing module and biology sensor, this biology sensor comprises the matrix of a slice optics plastic production, the lower surface of this matrix is optical flat, upper surface is the concavo-convex grating of polymkeric substance, the concavo-convex grating of this polymkeric substance is made up of the parallel groove of many spaced sets, and in groove, deposit one deck aptamer, this biology sensor is fixed on metal sample box, this optical attenuator and linear CCD sensor are positioned at the top of biology sensor successively, this data processing module is connected with linear CCD sensor, this cylindrical lens and spectroscope are positioned at the below of biology sensor successively, described semiconductor laser comprises red light semiconductor laser and green light semiconductor, this red light semiconductor laser and green light semiconductor are mutually an angle of 90 degrees and are arranged on spectroscopical bottom and right side, this spectroscope, cylindrical lens, optical attenuator, linear CCD sensor center is on same optical axis.
2. polymer grating aptamer biological monitor according to claim 1, it is characterized in that: the center of described metal sample box is provided with a rectangular depression region, the length in this rectangular depression region is 5 millimeters, width is 3 millimeters, be highly 1 millimeter, and the centre bit in rectangular depression region is equipped with a circle leads to light aperture, and the upper surface four direction of metal sample box respectively has a groove-shaped notch being used as to place and take out biology sensor, the width of each groove-shaped notch is 1.5 millimeters, and the degree of depth is 1 millimeter.
3. polymer grating aptamer biological monitor according to claim 1, it is characterized in that: the power of described red light semiconductor laser is 3 ~ 5 milliwatts, wavelength is 650 nanometers, output beam diameter is 1 ~ 2 millimeter, the power of described green light semiconductor is 5 ~ 10 milliwatts, wavelength is 532 nanometers, output beam diameter is 1 ~ 2 millimeter, the exiting surface of this ruddiness, green light semiconductor is apart from spectroscopical surperficial 10 ~ 20 millimeters, and the central axis of ruddiness, green light semiconductor and spectroscopical two mutually perpendicular deads in line.
4. polymer grating aptamer biological monitor according to claim 2, is characterized in that: described spectroscope is cube glass Amici prism, and the length of side is 10 ~ 15 millimeters; Described cylindrical lens is silica cylindrical lenses that is long 10 millimeters, wide 5 millimeters, focal length 10 millimeters, this cylindrical lens be arranged on spectroscopical directly over 5 millimeters of places; Described metal sample box is positioned over 10 millimeters of places directly over cylindrical lens, the center superposition of the focus of cylindrical lens and the logical light aperture of the circle of metal sample box.
5. polymer grating aptamer biological monitor according to claim 1, it is characterized in that: described optical attenuator is positioned over 30 millimeters, the top place of metal sample box, this optical attenuator is made up of two film polarizer, the diameter of two film polarizer is all 30 millimeters, one of them film polarizer maintains static, and another film polarizer can rotate freely to change laser light damping capacity around central axis.
6. polymer grating aptamer biological monitor according to claim 1, it is characterized in that: described linear CCD sensor is positioned over 10 millimeters, the top place of optical attenuator, the CCD length of this linear CCD sensor is not less than 25 millimeters, photosensitive element is 2700, photosensitive element is of a size of 11 × 11 microns, spectral response range 0.3 ~ 0.9 micron; Described optical attenuator and linear CCD sensor are coaxially arranged in the same box that can move left and right, and when measuring the diffracted light intensity of biology sensor, box is positioned at directly over metal sample box; When placing and take out biology sensor, box is positioned at the upper left side of metal sample box.
7. polymer grating aptamer biological monitor according to claim 1, is characterized in that: described data processing module comprises 12 A/D converters and single-chip microcomputer.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| GB2421076A (en) * | 2004-12-07 | 2006-06-14 | Univ Southampton | Identifiable particles and uses thereof |
| CN101317083A (en) * | 2005-07-08 | 2008-12-03 | Sru生物系统公司 | Photonic crystal biosensor structure and fabrication method |
| CN101548211A (en) * | 2006-12-05 | 2009-09-30 | 韩国电子通信研究院 | Guided mode resonance filter including high refractive index organic material and optical biosensor including the guided mode resonance filter |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| GB2421076A (en) * | 2004-12-07 | 2006-06-14 | Univ Southampton | Identifiable particles and uses thereof |
| CN101317083A (en) * | 2005-07-08 | 2008-12-03 | Sru生物系统公司 | Photonic crystal biosensor structure and fabrication method |
| CN101548211A (en) * | 2006-12-05 | 2009-09-30 | 韩国电子通信研究院 | Guided mode resonance filter including high refractive index organic material and optical biosensor including the guided mode resonance filter |
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