KR100835250B1 - A Self-emulsifying carrier and the preparation method thereof - Google Patents

Abstract

The present invention relates to a method for preparing a self-emulsifying carrier for receiving a lipophilic active ingredient used in cosmetics, pharmaceuticals or health functional food. More specifically, the self-emulsifying carrier of the present invention prepared by the manufacturing method consisting of a four-step simple process is characterized by spontaneously forming nanoparticles by the property of easily emulsifying or dispersing in the process of being solubilized. It enables the preparation of formulations containing high content of lipophilic components that overcome the low content of active ingredients which have been pointed out as a disadvantage of nanoparticle formulations, and can keep the active ingredients in a stable state for a long time and voluntarily Since the nanoparticles are formed, it can be applied to various applications such as cosmetics, medicine or health functional food.

Self-emulsifying carriers, liposomes, nanoparticles

Description

A self-emulsifying carrier and the preparation method

1 is a flow chart showing a manufacturing process of the self-emulsifying carrier of the present invention,

Figure 2 is an average particle distribution measured by applying the self-emulsifying carrier of the present invention by Example 1 and then subjected to dynamic light scattering (dynaymic light scattering),

3 is an average particle distribution diagram of a solution in which the self-emulsifying carrier of the present invention containing coenzyme Q10 (Coenzyme Q10) is dispersed in water such that coenzyme Q10 is 0.1% (w / v),

4 is an average particle distribution diagram of a solution in which the self-emulsifying carrier of the present invention containing oil soluble licorice extract as an active ingredient is dispersed in water such that the oil-soluble licorice extract is 1% (w / v).

5 is a diagram illustrating changes in the content of coenzyme Q10 when the self-emulsifying carrier of the present invention prepared with coenzyme Q10 as an active ingredient for 6 months at 45 ° C.,

Figure 6 is an analysis of the change in the content of the oil-soluble licorice extract when the self-emulsifying carrier of the present invention prepared with the oil-soluble licorice extract as an active ingredient for 6 months at 45 ℃.

The present invention contains a high content of lipophilic active ingredients used in cosmetics, medicines or health functional foods, and keeps them in a stable state to minimize the decrease in the content of the active ingredients before the solubilization, and the active ingredient by self-emulsifying It relates to a self-emulsifying carrier and a method for producing the same, characterized in that to produce nanoparticles containing.

In general, methods for solubilizing lipophilic components have been reported, such as coating with liposomes, suspensions with surfactants, fats or oils (Sosada M et al., EJLST , 105 (11), pp672-676, 2003; Fujita et al., Food Hydrocolloids , pp 429-434, 1993).

Conventional methods of making emulsions of nano-sized particles use liposomes, micelles, etc. in water to maintain emulsions by using surfactants such as lecithin, monoglyceride esters, sucrose esters, etc. , Particle size is controlled by a method using a homomixer or a microfluidizer, etc. [Suggy et al., BioPharm. , pp 10-14, 2001; Sun et al., Colloids and Surfaces , pp 95-104, 2002; Vladimir et al., Liposomes (second edition), pp 3-27, 2003]. To date, nano-sized phospholipid liposome cosmetics and its preparation method (Korean Patent Publication No. 10-2005-0055114), nano-sized phospholipid liposome composition comprising coenzyme Q10 and its preparation method (Korean Patent Publication No. 10-2006-0006988), A method for making a nano-sized liposome formulation in a stable solution of reduced coenzyme Q (Korean Patent Publication No. 10-2005-0116402), and the like, has been disclosed, and a self-associating polymer nanoparticle containing coenzyme Q10 and an external composition for skin containing the same ( In the patent of Korean Patent Publication No. 10-2005-0054012), a method for preparing nanoparticles using amphiphilic polymers is known. Nano-sized phospholipid liposome composition including coenzyme Q10 among the patents designed to accept the lipophilic component and increase the stability, and the method of producing the coenzyme Q10, which is an active ingredient formulated, is 0.1 ~ 1% (w / v), especially at concentrations above 1% (w / v), it is difficult to formulate nanoliposomes due to precipitation before emulsification, and the content of coenzyme cue 10 in a stable solution of reduced coenzyme Q is 0.1% (w / v). No formulation of high content was done. As described above, it was not possible to prepare nanoliposome formulations containing high lipophilic components while maintaining the stability of the formulations in conventional methods.

Such a method is generally a water in oil type (W / O type) emulsion in which the lipophilic component is used to maintain the stability of the formulation and the stability of the lipophilic component by using an emulsifier such as lecithin and a surfactant. For example, particles are contained in the fatty acid group of the liposome, or micelles are formed by a surfactant treated with a complex to maintain a dispersed state.

Liposomes are colloidal structures with an internal water phase surrounded by one or more phospholipid layers. In general, liposome systems exhibit problems of stability during storage, preparation methods with poor reproducibility, and difficulties in incorporating and maintaining active ingredients.

In general, liposome formulations produced by lecithin can increase stability in the process of solubilization, but are not themselves media that increase stability. The increase in stability can be achieved by inducing electrostatic repulsion on the outside of the particles with anionic and cationic surfactants and ionic polysaccharides, or by making modified liposomes conjugated with a polymeric material. However, the emulsion formed to increase the stability may also increase the average size of the particles during long-term storage, it is difficult to completely exclude the separation and destruction of the component in the case of the lipophilic active ingredient having an antioxidant effect. In the case of water-based emulsions, it is difficult to increase the ratio of lipids to be solubilized, that is, lipophilic components, and in order to increase the ratio of lipophilic components, the addition of lecithin / surfactant or addition of an additional stabilizer is required. There may be a concern of skin toxicity, there is a problem of causing a specific odor and bitter taste when used in foods, the formulation is difficult to apply because the viscosity is increased and the usability is limited, stability may occur.

Nanoemulsion prepared by emulsifying emulsified particles in nanounits, liposomes prepared using phospholipid self-association phenomenon, solid lipid nanoparticles obtained by nanoparticles of solid lipids, and polymeric nanoparticles stabilized by surfactants Although the research on the nanoparticles has been widely conducted, the nanoparticles are difficult to be prepared at the nanoscale size due to the colloidal instability mechanism due to the small size, and various kinds of surfactants, additives, dispersants, or stabilizers are added in combination. Since it has to be used and does not have a nano size particle size voluntarily, it has been manufactured through a process requiring high energy consumption such as high pressure emulsification. In addition, all nanoparticles prepared from the above techniques are commonly unstable due to colloidal instability mechanisms such as Ostwald ripening, precipitation, flocculation, etc. In particular, colloidal instability increases with increasing solid content. This rapid increase has been a disadvantage in that it cannot contain a high content of nanoparticle dispersions (21st Proceedings of IFSCC International Congress 2000, p442-458, 2000).

Coenzyme Q10 is called ubidecarenone, ubiquinone 10, and the like, and has been used in medicine as a metabolic cardiac medicine due to its specific physiological action. In addition, it can be used for food use in Korea, and application in the field of health food is expected. In particular, the recent research results of coenzyme Q10 have progressed remarkably, and in addition to myocardial protective action, carcinogenesis prevention, anti-aging action, blood LDL oxidation inhibition, blood pressure increase inhibition, and oxygen utilization efficiency in ischemic myocardium, Improvement, reactivation of ATP synthesis of myocardial mitochondria and improvement of cardiac function have been reported. However, coenzyme Q10 is a yellow to orange solid at room temperature, hardly soluble in water and alcohol, and insoluble in oil, so it is very low in versatility in food use, especially in beverage applications. There was a drawback to receiving.

As a preparation method of coenzyme Q10, the preparation method of the aqueous liquid preparation of a fat-soluble substance which mixes coenzyme Q10, an emulsifier, a polyhydric alcohol, and water, and then autoclaves (Japanese Patent Laid-Open No. 2000-212066), polyoxyethylene sorbitan mono A method of solubilizing using oleate (Japanese Patent Publication No. 2001-504343) and the like are known. However, although the aqueous liquid preparation prepared according to the former method can temporarily prepare coenzyme Q10 as an aqueous liquid by an ultra high pressure treatment of 1000 kg / cm 2, the stability of the turbidity or coenzyme Q10 precipitates as time passes. do. In the latter method, polyoxyethylene sorbitan monooleate is used, and such an ethylene oxide-based surfactant is preferable for use in food applications because of its high production of by-products during the synthesis reaction. Not.

Therefore, the present inventors have studied a quick and inexpensive manufacturing process consisting of a simple procedure for the preparation of a formulation for overcoming the disadvantages of the solubilized formulation of the lipophilic active ingredient including coenzyme Q10. The method for producing a self-emulsifying carrier established by the present inventors is a method for producing a self-emulsifying carrier having a simple, rapid and inexpensive process that can be applied to an industrial production process. Compared with a conventional soluble formulation of a lipophilic component, it is possible to increase the active ingredient content without adding an additional stabilizer, confirming that the active ingredient can be maintained in a stable state for a long time before solubilization, thereby completing the present invention. It was.

Accordingly, an object of the present invention is to increase the lipophilic active ingredient content without the addition of additional agents or stabilizers, to have a high stability by minimizing the decrease of the component content during long-term storage, spontaneous nano-sized liposomes in the process of solubilization Or to provide a method for producing a self-emulsifying carrier to form a micelle and a self-emulsifying carrier produced thereby.

In order to achieve the above object, the present invention comprises a first step of homogeneously dissolving a lipophilic active ingredient in a nonpolar oil; A second step of adding lecithin to the polyol or polyhydric alcohol, adding a surfactant, stirring and warming to homogeneously disperse it; When the lecithin is sufficiently homogeneously dispersed, the third step of pre-emulsifying by gradually adding the active ingredient prepared in the first step to the dispersed lecithin and passing the pre-emulsion through a high pressure emulsifier to generate a self-emulsifying carrier Provided is a method of preparing a self-emulsifying carrier containing a lipophilic active ingredient, comprising the final step.

The present invention also provides a self-emulsifying carrier prepared by the above production method, preferably a lipophilic carrier comprising a lipophilic active ingredient, a nonpolar oil, lecithin, a polyol and a surfactant.

The self-emulsifying carrier of the present invention essentially comprises a lipophilic active ingredient: nonpolar oil: lecithin: polyol: surfactant: 0.1-20: 0.2-20: 0.2-5: 30-97: 0.05-2.0 wt%, more preferably Is a lipophilic active ingredient: nonpolar oil: lecithin: polyol: 0.15 to 20: 1 to 20: 0.3 to 5: 40 to 97: 0.1 to 1.75% by weight, very preferably lipophilic active ingredient: nonpolar oil: Lecithin: Polyol: Characterized in that it contains 1 to 20: 2 to 20: 0.5 to 5: 50 to 96.5: 0.2 to 1.5% by weight surfactant.

Hereinafter, the present invention will be described in detail.

The present invention provides about 0.1 to 10 times, preferably about 0.5 to 5 times the volume of alcohols, nonpolar oils such as acetic acid, lactic acid, glycerin, propylene glycol or caprylic / capric triglycerides and solvents thereof. Warming to at least 40 ° C., preferably at least 60 ° C. for 10 minutes to 1 hour, preferably 30 to 40 minutes, by adding a lipophilic active ingredient and an antioxidant to the mixture, preferably caprylic / capric triglycerides A first step of homogeneously dissolving the lipophilic active ingredient by stirring with the same; Polyols or polyalcohols such as polyethylene glycol, propylene glycol or copolymers and derivatives thereof, dipropylene glycol, sorbitol, glycerol, molasses, butylene glycol, pentanediol, hexanediol, heptanediol, octanediol, nonanediol and decandiol, and the like, Preferably, the content of lecithin, preferably phosphatidylcholine, in a weight ratio of about 0.1 to 5 times, preferably about 0.25 to 3 times, of the lipophilic active ingredient in the pentanediol or glycerol solvent is about 50 to 90% (w / w). Soybean purified soybean lecithin is added, and it is heated and stirred by adding about 0.1 to 5 times the weight ratio of nonionic surfactant, preferably about 0.2 to 1 times the weight ratio of Tween 80 ^TM . A second step of homogeneously dispersing the active agent; The third step of slowly adding and stirring the active ingredient dissolving solution of the first step to the homogeneously dispersed lecithin liquid and stirring the pre-emulsion in a microfluidizer at about 1000 to 1500 bar, preferably The self-emulsifying carrier of the present invention, characterized in that it comprises the step of passing 1-6 times, preferably 3-4 times, at a pressure of 1100-1300 bar to produce a self-emulsifying carrier containing the lipophilic active ingredient. It provides a manufacturing method to obtain.

In addition, the present invention provides a highly concentrated microparticle self-emulsifying carrier in which a lipophilic carrier prepared by the above-described manufacturing method, preferably a lipophilic active ingredient including a lipophilic active ingredient, a nonpolar oil, lecithin, a polyol and a surfactant is dissolved. to provide.

The self-emulsifying carrier is a particle of microparticle size containing a lipophilic active ingredient, and is characterized in that when emulsified with a sufficient aqueous medium, particles are emulsified to form particles of nanoparticle diameter.

The term “self-emulsifying carrier” of the present invention is a liposome precursor concentrate composition, which is generally characterized by a particle diameter of about 1.0 to 5.0 μm on average, when mixed with a sufficient aqueous medium, for example, 0.1 to 5 active ingredients. It refers to a concentrated composition capable of forming an emulsion or microemulsion composed of liposomes having an average particle size of about 10-200 nm by self-emulsification when solubilized in% (w / v).

The lipophilic active ingredient applicable to the self-emulsifying carrier of the present invention is astaxanthin, beta-carotene, lycopene, lutein, kaempferol, coenzyme Q10 (Coenzyme Q10). ), Polyphenol, Vitamin E and its derivatives, Vitamin A and its derivatives, Provitamin D3 and its derivatives, Ginsenoside, Linoleic acid, Gamma-linoleic acid, Doco Docosahexaenoic acid (DHA), Eicosapentaenoic acid (EPA), Grape seed oil, Resveratrol, Beta-sitosterol, Genistein Includes all lipophilic components including oil soluble licorice extract or mixtures thereof.

Oil-soluble licorice extract of the present invention is licorice ( Glycyrrhiza uralensis FISCH.) or the roots of the roots of other legumes in Leguminosae with anhydrous ethanol and left for one day to extract the extract twice, fractionated it with ethyl acetate and decompressed the extract. By concentrating and drying, oil-soluble licorice extract containing at least 35% of glabridin can be obtained.

The nonpolar oil of the present invention may be used a variety of nonpolar oils known in the art, preferably hydrocarbon-based oils such as hexadecane and paraffin oil; Synthetic synthetic oils; Silicone oils such as dimethicone and cyclomethicone type; Animal and vegetable oils such as sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil; Ethoxylated alkyl ether oils; Propoxylated alkyl ether oils; Sphingoidoid lipids such as phytosphingosine, sphingosine and sphinginine; Cerebroside; cholesterol; Cytosterol; Cholesteryl sulfate; Cytosteryl sulfate; C10-40 fatty alcohols and mixtures thereof; Caprylic / capric triglycerides, more preferably caprylic / capric triglycerides.

Lecithin of the present invention is egg yolk lecithin, purified soy lecithin, phosphatidylcholine, risphospatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin, phosphatidylglycerol, stearylamine, dicetyl phosphate, ceramide phosphoryl glycerol, phosphatidylinositol and its hydrolysis Mixtures of other fatty acids and the like, preferably purified soy lecithin.

Lecithin of the present invention is a hydrogenated or non-hydrogenated type, saturated and unsaturated lecithin having a content of 50 to 90% (w / w) of phosphatidylcholine, and 0.1 to 10% (w / v) of the total self-emulsifying carrier content. It may be added as, it is preferably characterized in that it is added at 0.5 to 5% (w / v).

The surfactant of the present invention may use all of various surfactants known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, natural surfactants or polymeric surfactants. And, preferably, a nonionic surfactant and polysorbate, Tween 20 ^™ , Tween 60 ^™ or Tween 80 ^™ .

The surfactant of the present invention is an auxiliary surfactant of lecithin, and is characterized in that it is used in a mass ratio of 0.1 to 5 times, preferably 0.2 to 1 times, relative to the amount of lecithin used.

Polyols or polyhydric alcohols of the present invention are polyethylene glycol, propylene glycol or copolymers and derivatives thereof, dipropylene glycol, 1,3-butylene glycol, methyl propanediol, isopropylene glycol, pentylene glycol, sorbitol, glycerol, erythritol, Xylitol, sorbitol, molasses, butylene glycol, pentanediol, hexanediol, heptanediol, octanediol, nonanediol and decandiol, characterized in that there is no restriction on where the hydroxyl group (-OH group) is bound do.

The self-emulsifying carrier prepared according to the present invention was subjected to a self-emulsifying carrier containing 0.1% (w / v) of coenzyme Q10 or 1% (w / v) of oil-soluble licorice extract under accelerated conditions of 45 ° C and 75% relative humidity. After 6 months, the content of the active ingredient was confirmed to be maintained within 5%, and when contacted with the aqueous solution was found to form nanoparticles with an average particle diameter of about 10 to 200 nm, preferably 30 nm to 150 nm. .

Therefore, the self-emulsifying carrier of the present invention can minimize the decrease in the content of the lipophilic active ingredient, it is characterized in that it has the effect of enabling long-term storage until the active ingredient is solubilized.

In addition, the self-emulsifying carrier of the present invention may further include pharmaceutically acceptable additives such as dissolution aids, viscosity regulators, antioxidants, stabilizers, preservatives and the like.

Antioxidants or stabilizers of the invention include vitamin E and derivatives thereof, including vitamin E and vitamin E acetate, vitamin C and derivatives thereof, including vitamin C and vitamin C sodium salts, or catechins.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Example  1-4. Coenzyme Q10 Preparation of self-emulsifying carrier containing

Self-emulsifying carriers containing coenzyme Q10 were prepared in the ratio of Table 1 below. Coenzyme Q10 (CO060306, Xi'an Hao Tian Bio-engineering Technology, China) was dissolved in caprylic / capric triglyceride (MCT, Esterchem) at a concentration of 1-20% (w / v). Tocopheryl acetate (Wako, Japan) was dissolved with coenzyme Q10 for stability of the formulation. Soybean refined lecithin (S75-3, Lipoid) and Tween 80 (Tween 80 ^™ ; 56622, Uniqema) were homogeneously dispersed using a homomixer (SSC811CA, Matsushita, Japan). The dissolved coenzyme Q10 was slowly added to the lecithin-dispersed glycerol solution, and stirred in a maximum pre-emulsion state using a homomixer, and then a high pressure emulsifier (Microfludizer; M-710, Microfluidics, US) was used. Repeated treatment three times at 1200 bar using a self-emulsifying carrier containing the coenzyme Q10.

 Component (%, w / v) Example 1 Example 2 Example 3 Example 4  Glycerol 96.1 82.2 70.9 53.3  lecithin 0.5 2.0 3.0 5.0 Tween 80 ^TM 0.2 0.6 0.9 1.5  Coenzyme Q10 1.0 5.0 10.0 20.0  Tocopheryl acetate 0.2 0.2 0.2 0.2  Caprylic / Capric Triglycerides 2.0 10 15.0 20.0

Example  5 ~ 8. Preparation of self-emulsifying carrier containing oil-soluble licorice extract

Self-emulsifying carriers containing oil-soluble licorice extracts were prepared in the ratios of Table 2 below. Oil-soluble licorice extract (OSLP-033155, Bioland) was warmly dissolved in caprylic / capric triglyceride (MCT, Esterchem) at a concentration of 1-20% (w / v), and toco for stability of the formulation. Peryl acetate (Tocopheryl acetate; Wako, Japan) was dissolved with oil soluble licorice extract. Soybean purified lecithin (S75-3, Lipoid) and Tween 80 (Tween 80 ^TM ; 56622, Uniqema) were heated to 1,5-pentanediol (Sigma, St-Louis, MO, USA) warmed above 60 ° C. homomixer; SSC811CA, Matsushita, Japan) to homogeneously disperse. The dissolved coenzyme Q10 was slowly added to the lecithin-dispersed 1,5-pentanediol solution, stirred in a maximum pre-emulsion state using a homomixer, and then a high pressure emulsifier (Microfludizer; M-710, Microfluidics, US) was used three times at 1200 bar to prepare a self-emulsifying carrier containing the coenzyme Q10.

 Component (%, w / v) Example 5 Example 6 Example 7 Example 8  1,5-pentanediol 96.1 82.2 70.9 53.3  lecithin 0.5 2.0 3.0 5.0 Tween 80 ^TM 0.2 0.6 0.9 1.5  Soluble Licorice Extract 1.0 5.0 10.0 20.0  Tocopheryl acetate 0.2 0.2 0.2 0.2  Caprylic / Capric Triglycerides 2.0 10 15.0 20.0

Experimental Example  1. Self-emulsifying carrier Droplet size  And distribution measurements

An aqueous solution was prepared by diluting the self-emulsifying carriers prepared in Examples 1 to 8 with 0.1 to 5% (w / v) of lipophilic active ingredient. The size and distribution of nanoparticles formed in the dilute aqueous solution of the self-emulsifying carrier and the self-emulsifying carrier, which are the microparticles prepared in Examples 1 and 5, were measured using a light scattering particle analyzer (Zetasizer Nano-ZS, Malvern, UK). Particle distribution diagrams for the diluted aqueous solutions of Examples 1 and 1 and the diluted aqueous solutions of Example 6, respectively, measured by the dynamic tube scattering method are shown in FIGS. 2 to 4. The average particle diameter is the average particle diameter calculated from the Stokes-Einstein equation, and the results are shown in Table 3.

As shown in Table 3, it was confirmed that the self-emulsifying carrier prepared by the present invention forms fine particles having an average particle diameter of about 46 nm to 125 nm when contacted with an aqueous solution.

Average particle size (nm)  Example 1 2399  Example 5 1987  Dilute aqueous solution of Example 1 (coenzyme Q10 content 0.1%) 114  Dilute aqueous solution of Example 2 (coenzyme Q10 content 1%) 121  Dilute aqueous solution of Example 3 (coenzyme Q10 content 2%) 119  Dilute aqueous solution of Example 4 (coenzyme Q10 content 5%) 125  Dilute aqueous solution of Example 5 (soluble licorice content 0.1%) 48  Dilute aqueous solution of Example 6 (soluble licorice content 1%) 54  Dilute aqueous solution of Example 7 (soluble licorice content 2%) 46  Dilute aqueous solution of Example 8 (soluble licorice content 5%) 47

Experimental Example  2. Measurement of stability of self-emulsifying carrier active ingredient

In order to test the active ingredient stability of the self-emulsifying carrier under accelerated conditions, it was placed in an incubator (NDO-600SD, Eyela, Japan) and examined for stability at 45 ° C. for 6 months.

Determination of the content of active ingredients was carried out using High Performance Liquid Chromatography (HPLC); 717 plus autosampler, 1525 binary pump and 2487 dual wavelength UV detector, Waters, US; and the column was Mightysil RP-18 (250 * 4.6 mm, 5). Μm, Kanto chemical, Tokyo, Japan) and UV 275 nm for the detection spectrum. Ethanol was used for the mobile phase, and the flow rate was 1.0 ml / min. As standard, coenzyme Q10 (Sigma, St-Louis, MO, USA) reagent and glabridine (Glabridin, Aldrich, St-Louis, MO, USA) were used in methanol at a concentration of 1 mg / ml. 1, 0.1, 0.01 ㎎ / ㎖ sample solution to prepare a calibration curve was prepared using a standard solution for the calibration curve, the sample of the present invention in ultrapure water so that the concentration of the active ingredient is about 1 mg / ㎖ Diluted and analyzed in the same manner as the standard, the experimental results are shown in Figures 5 and 6.

As shown in FIG. 5 and FIG. 6, it was confirmed that the content decrease of the active ingredient after 5 months was maintained at 5% or less under the accelerated conditions of 45 ° C. and 75% relative humidity. It was found that the emulsion carrier showed very good stability to keep the active ingredient stable.

As described above, the self-emulsifying carrier obtained by the preparation method of the present invention is an intermediate in which a lipophilic component is solubilized, contains a high content of a lipophilic active ingredient, and has excellent formulation stability, thus enabling long-term storage before use. Once solvated, it can form nanoliposomes with an average particle size of 10-200 nm by a self-emulsifying system. In addition, the self-emulsifying carrier of the present invention is stable until solubilization, and the nanoliposomes, which are solubilization products, promote the absorption and permeation of the active ingredient, and thus various drugs, cosmetics, health functional foods, foods, food additives, etc., depending on the intended use Applicable in dosage form.

Claims (16)

Coenzyme Q10 and oil-soluble licorice extract components in caprylic / capric triglyceride and tocopheryl acetate for 10 minutes to 1 hour or more at 40 ° C. step; Adding a soybean tablet lecithin having a phosphatidylcholine content of 50-90% (w / w) to glycerol or pentadiol, and adding a supplementary surfactant Tween80 to a homogenous dispersion by heating and stirring; When the soybean lecithin of the second step is sufficiently homogeneously dispersed, a solution to which the active ingredient prepared in the first step is added (lipophilic active ingredient: nonpolar oil: lecithin: polyol: surfactant 0.1-20: 0.2-20 : 0.2-5: 30-97: 0.05-2.0% by weight), the third step of pre-emulsifying and passing the pre-emulsion three to four times at a pressure of about 1100 to 1300 bar through a microfluidizer And a final step of producing a self-emulsifying carrier which is a particle having a micro particle diameter, and when it is solubilized, it is self-emulsified and 45-125 nm of nanoparticles containing crude enzyme Q10 and oil-soluble licorice extract active ingredient 0.1-5% (w / v). A method of producing a self-emulsifying carrier comprising coenzyme Q10 and oil-soluble licorice extract components characterized by forming liposome particles of particle size. delete delete delete delete delete delete delete delete delete delete delete delete delete delete Self-emulsifying carrier produced by the method of claim 1.

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