KR100764813B1 - Composition comprising extract of herbal mixture for prevention or treatment of osteoporosis and fracture - Google Patents

Abstract

The present invention relates to a composition for preventing and treating bone diseases, including mixed herbal extracts of lettuce, goji berry, medicinal beans and antler, and more particularly, to a mixed herbal extract for inhibiting osteoporosis and increasing bone formation in uterine extract mice. The composition comprising the mixed herbal medicine extract of the present invention can safely improve symptoms of bone disease without changing hormones, and thus can be usefully used for preventing and treating bone diseases by replacing existing therapeutic agents.

Upper lobe, wolfberry, medicinal beans, antler, osteoporosis

Description

Composition comprising extract of herbal mixture for prevention or treatment of osteoporosis and fracture}

1 is a diagram showing the change in weight according to the administration group,

2 is a diagram showing the change in weight of the uterine tissue according to the administration group,

Figure 3 is a diagram showing the change of the small bone area according to the administration group.

The present invention relates to a composition for preventing and treating bone diseases, including mixed herbal extracts of lettuce, wolfberry, medicinal beans and antler, and more particularly, to a mixed herbal extract extract for inhibiting osteoporosis and increasing bone formation in uterine extract rats. will be.

Morus Folium (Mori Folium) is the dried leaf of the mulberry, Morus alba L. belonging to the mulberry family and related plants.目) is effective. The main ingredient is rutin, and other ingredients include ecdysterone, inosterone, insitosterol, moracetin, scopolin, scopoletin, and iso Isoquercitroside, linalool, benzyl alcohol, myoinositol, eugenol, butylamine, acetone, trigonelline, Choline, chlorogenic acid, fumaric acid, folic acid, formyltetrahydrofolic acid and the like are known.

Pharmacological action has been reported antibacterial action, hypoglycemic action, smooth muscle relaxation action, anti-inflammatory action, diabetes treatment action, etc., but the action on bone disease is not known. Toxicity of the injection of upper lobe to mice is very small and the safe dose when intraperitoneally administers 0.1 g / ml injection of lobe to 20 g mice is 250 times that of humans. When the liver, kidney and lung were observed on day 21 after 60 times of human dose was intraperitoneally injected into mice, no damage was observed (Han Pharmacology, Kim Ho-cheol, Moon-dang, 2001, p. 105 ~ 106)

Lycii Fructus is a dried fruit of Lycium chinense Mill or other cognate, a chronic shrub belonging to the branch family. In China, the origin is Ninghai Goji ( L. barbarum L.). Herbal efficacy of self-interpolated nomenclature (滋腎 補肝 明目), Yunjeoljihae (潤肺 止咳) efficacy of the efficacy. The ingredient mainly contains amino acids, trace elements, polysaccharide betaine, vitamins B1, B2, C, folic acid and pigments. Its main ingredient is about 1% of polysaccharides and betaines, and contains carotene. Polysaccharides of goji berry is known as the main active ingredient.

Pharmacological action is known as immune enhancing and regulating action, hematopoietic function promoting action, anticancer action, anti-aging action, lipid lowering action and liver function protection action, hypoglycemic action and the like.

The toxicity of goji berry is very low, and when the goji berry extract is injected subcutaneously into mice, LD ₅₀ is 8.32 g / kg and the main ingredient, betaine 18.74 g / kg, is more toxic.

Medicinal beans are called Seomokmok or Ratsui Bean. The scientific name is Glycine max Merr. Leguminosae. Its origin is black soybean ( Semen Glycine Nigrae ), which contains trace amounts of genistein, biochanin, and daidzein (Korean Pharmacopeia Species, http://www.kfda.go.kr ). The main components are isoflavones and saponins, and p-Coumaric acid, Coumestrol, Glyceollin IV, Tocopherol and Perco Perlolyrine, Lutein, Vitamin A, Vitamin B2, Biotin, Isopropylamine, O-β-D-glucopyranosylzetin, 13-cis-β-carotene, Various amino acids , Isoliquiritigenin, Formononetin, Ononin, Genistein, Isoquercitrin, Fistine, 4 ', 5,7-trimethoxy Trimethoxyisoflavone, Genistin, 4 ', 5,7-trianetoxyisoflavone, Diedzein, 4', 7-diacetoxyisoflavone, 4 ', 7-Dimethoxyisoflavone, Astragalin, Kempferol-3-O-β-D-Soforoside (Kaempferol-3-O- β-D-sophoroside, Quercetin, Rutin, Kaempferol, Sophoraflavonoloside, Daidzin, Glycitin, Soiasaponin ( Soyasaponin I ~ VI), Soiasapogenol A, Soyasapogenol E, Lupeol, Soiasaponin A3 ~ A6, Ergost-4-en-3-one, Stigmast-4-en-3-one, Campesterol and Glycinoprenol 10 are also included.

Cervi Cornu Pantotrichum (Cervi Cornu Pantotrichum) is a cut and dried non-oskeletal husk of the ermine family, Maer ( Cervus nippon Temminck) or Manok ( C. elaphus L.) and cohort. Korean medicine has bosinyangikjeong blood (補腎 陽 益 精 血), Kang Geungol (强筋 健 骨) of the efficacy. The component contains several kinds of amino acids, among which the highest content of proline, lysine, and serine. The active ingredient of the deer antler is the two components of heavy ethanol extract has a coercive action. One of them is lysophosphatidylcholine (LPC), which includes myristic acid, palmitic acid, pentadecanoic acid, palmitoleic acid, and stearic acid. It is composed of 8 fatty acids, including oleic acid, linoleic acid and daturic acid. The butanol fraction contains uracil, hypoxanthine, urea, uridine, niacin, and the like. The amine benzoyl aldehyde, hypoxanthine, and uracil in antler all have the effect of inhibiting the strong monoamine oxidase (MAO) activity. Deer Antlers contain a large amount of amines, including spermine, spermidine, and putrescine. In the middle and bottom of the antler, ossification is much progressed and the content of spermidine is reduced. Deer Antlers account for the smallest percentages in terms of weight percentage and contain spermidine and putrescine in the amines. In addition, the antler contains polysaccharides such as chondroitin sulfate A, gangliosides, androgens, and estradiol. Recently, peptides are important as active ingredients in antler, but peptide compounds with anti-inflammatory activity have been isolated. Pharmacological action is known to promote protein and nucleic acid synthesis, hematopoietic promotion, cardiovascular effects, immune enhancement, sexual enhancement, human work efficiency and antistress, anti-aging, anti-ulcer .

Toxicity of pantocrine (LD ₅₀ ) was 117-123 mg / kg in mice and 87.8-91 mg / kg in rats. Poisoned animals have symptoms of trembling, calming, asthma, tears, and so on. In the subacute toxicity test, 3, 9 and 27 ml / kg of pantoclean daily intraperitoneally to rats for 12 weeks showed clear fibrous peritonitis at 9 and 27 ml / kg. Endocrine changes were not apparent. Higher doses of pantocrine slightly lower levels of erythrocyte volume, hemoglobin, albumin, albumin / globulin. Pantoclean has no antigenicity or teratogenic action.

Osteoporosis is prone to fractures even with small impacts, as bone mineralization is reduced, resulting in thinner bone dense, resulting in a wider bone marrow cavity, and weakened bones as symptoms progress. . Bone mass is influenced by several factors, including genetics, nutrition, hormone changes, differences in exercise and lifestyle, and the causes of osteoporosis include old age, lack of exercise, low weight, smoking, low calcium diet, menopause, Ovarian ablation and the like are known. On the other hand, although there are individual differences, blacks have lower bone resorption levels than whites, resulting in higher bone mass. Usually bone mass is highest at 14-18 years of age and decreases by about 1% per year. Especially in women, bone reduction continues after 30 years of age, and when menopause reaches bone growth rapidly due to hormonal changes. In other words, estrogen concentration rapidly decreases at the end of menopause, when a large amount of B-lymphocytes are produced, as in the case of IL-7 (interleukin-7), and the B cell precursor (pre-B) is added to the bone marrow. cells) accumulate and thereby increase the amount of IL-6, thereby increasing the activity of osteoclasts, which eventually leads to a decrease in bone mass.

As described above, osteoporosis is unavoidable for elderly people, especially postmenopausal women, and as the population ages in developed countries, interest in osteoporosis and its therapeutics is gradually increasing. It is also known that there is a market of about $ 130 billion related to the treatment of bone diseases worldwide and is expected to increase further. Therefore, many research institutes and pharmaceutical companies around the world are investing heavily in the development of bone disease treatments. have.

Materials currently used for the treatment of osteoporosis include estrogen, androtic anabolic steroids, calcium preparations, phosphates, fluoride preparations, ipriflavones, and vitamin D ₃ . In 1995, Merck, USA, used aminobisphosphonate, and in 1997, Lilly Co., USA, used raloxifene, which acts as a selective estrogen receptor modulator (SERM). It was developed as a treatment.

On the other hand, conventional osteoporosis therapeutic agents are mostly estrogen-based substances, the estrogen-based substances are known to exhibit side effects such as cancer, gallstones, thrombosis when administered for a long time. Osteoporosis, however, cannot be treated with short-term administration of the drug, and long-term administration of the drug is essential. Therefore, there is a need for a new substance that does not have such side effects even when the drug is administered for a long time and has a good efficacy enough to replace estrogen.

The patent for a composition for preventing and treating osteoporosis comprising herbal extracts is a Chinese herbal medicine that improves bone powder and skeletal function. Composition for improving and preventing osteoporosis, including baekbokyeong, ginseng, antler, old plate runner, sine, etc. (Korean Patent Publication No. 2005-0072625), 3.75-15 parts by weight of larvae, 3-15 parts by weight and 3-20 weights of antler There is a patent for a method of manufacturing a herbal composition for treating osteoporosis, comprising a portion (Korean Patent No. 472408), a mixed herbal extract and a pharmaceutical composition for preventing or treating osteoporosis comprising the same (Korea Patent Publication No. 2003-0031418). However, the above patents have a low therapeutic effect on bone diseases even if they contain only some of the mixed herbal medicines of the present invention or similar compositions.

Accordingly, the present inventors completed the present invention by revealing that the mixed herbal extracts of the upper lobe, goji berry, medicinal soybean and antler of the present invention can be effectively used to prevent and treat osteoporosis because it significantly increases the area of the small bone in ovarian depleted rat model It was.

An object of the present invention is to provide a composition for the prevention and treatment of bone diseases, including osteoporosis and fracture using the mixed herbal extracts of the upper leaves, gojija, medicinal beans and antler, which are natural ingredients without side effects.

In order to achieve the above object, the present invention provides a mixed herbal extract of lettuce, wolfberry, medicinal beans and antler having a bone disease prevention and treatment effect.

In addition, the present invention provides a pharmaceutical composition for preventing and treating bone diseases containing the mixed herbal medicine extract as an active ingredient.

In addition, the present invention provides a health food containing the mixed herbal medicine extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a mixed herbal extract of lettuce, wolfberry, medicinal soybean and antler with the effect of preventing and treating bone disease.

The solvent for extraction is water, alcohol or mixtures thereof, 5 to 95% water and 95 to 5% alcohol and the alcohol is preferably ethanol. The present inventors extracted and filtered the mixed herbal medicine powder, and the filtrate was concentrated under reduced pressure and lyophilized to obtain a mixed herbal medicine extract. In a preferred embodiment of the present invention, the mixed herbal medicine extract was mixed into 4 parts by weight of the upper leaves, 4 parts by weight of wolfberry, 4 parts by weight of medicinal beans and 1 part by weight of antler.

Bone diseases that can be prevented or treated with the mixed extract of the present invention include, but are not limited to, osteoporosis, fracture, osteopenia, rickets, osteomalacia, and the like.

In addition, the present invention provides a pharmaceutical composition for preventing and treating bone diseases containing the mixed herbal medicine extract as an active ingredient.

In order to examine the effects of the mixed herbal medicine extract of the present invention on bone disease, animal experiments were conducted in rats in which type I osteoporosis occurs after menopause. Specifically, the sham group, which performed all operations except ovarian extraction, the control group without ovaries and drug administration, and the control group with ovarian extraction and estradiol, a mammalian estrogen ( After classification into E2), the Siamese group and the control group were orally administered drinking water, and the E2 group was intraperitoneally injected with 17β-estradiol. Thereafter, the mixed extract of the present invention was orally administered.

As a result, the control group showed a sharp increase in body weight compared to the Siamese group, and the mixed extract administration group showed a similar weight gain as the control group. The control drug group (E2) showed a moderate weight gain phenomenon between the Siamese group and the control group (see FIG. 1). In addition, the weight of the uterus was sharply decreased in the control group compared to the Siamese group (normal group), and the group administered with E2 was recovered to 1/2 or more of the normal group. The mixed extract administration group was found to be no difference from the control group (see FIG. 2).

Therefore, the mixed extract of the present invention did not change the body weight and the weight of the uterus compared to the control group and the control drug group was confirmed to be safe without changing the hormone in the body.

In addition, morphometric analysis was performed on the lumbar and tibia in each group. Specifically, the area of each shochu was calculated from the lumbar and tibia and the area of the shochu bone was measured therefrom.

As a result, the control group extracted from the ovary was confirmed that the osteoporosis was induced by reducing the area of the sub-catheter bone compared to the Siamese group (normal group), it was confirmed that after the administration of E2 or mixed extracts the area of the sub-catheter bone increased (see Figure 3). ).

Therefore, the mixed extract of the present invention does not change the weight and weight of the uterus, but suppresses osteoporosis and promotes bone formation, so it can be used as a safe substance that does not cause hormone changes in the body and can be used for treating and preventing bone diseases. It was.

The pharmaceutical composition for preventing or treating bone diseases of the present invention contains the mixed extract as an active ingredient. The mixed extract may be administered orally or parenterally during clinical administration and may be used in the form of a general pharmaceutical formulation. Preferred pharmaceutical preparations include tablets, hard or soft capsules, oral preparations such as liquids, suspensions, and parenteral injections such as injectable solutions or suspensions, and these pharmaceutical preparations are conventionally pharmaceutically acceptable. Carriers such as excipients, binders, disintegrants, lubricants, solubilizers, suspending agents, preservatives or extenders in the case of injectables, stabilizers, preservatives, dissolution aids, buffers or isotonic agents in injections. A topical agent etc. can be used.

In the pharmaceutical composition for preventing or treating bone diseases of the present invention, the dosage of the mixed extract may be determined by a specialist according to various factors such as the severity of the disease, the condition of the patient, age, sex and complications, but in general, Body weight of about 50 kg), may be administered at a dose of 50 mg to 30 g per day, preferably 500 mg to 5 g. In addition, the unit dosage is to contain a daily dose of the mixed extract or a dose of 1/2, 1/3 or 1/4 thereof, it may be administered 1 to 6 times a day.

As the raw material of the mixed extract of the present invention, the upper leaves, goji berry, medicinal beans and antler are secured as natural medicines, and in particular, when the mixed extract of the present invention was orally administered to rats, the toxicity test was performed. % Lethality (LD ₅₀ ) was found to be a safe substance with at least 10 g / kg of the mixed extract.

In addition, the present invention provides a health food for preventing bone disease containing a mixed extract as an active ingredient.

When the extract of the present invention is used as a food, the extract may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The blending amount of the active ingredient can be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment). In general, the mixed extract of the present invention may be added in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight based on the raw materials in the manufacture of food or beverage. The effective dose of the mixed extract of the present invention may be used in accordance with the effective dose of the pharmaceutical composition, but may be less than the above range in the case of long-term intake for the purpose of health and hygiene or for health control. It is evident that the component can be used in an amount above the range because there is no problem in terms of safety.

There is no particular limitation on the kind of food. Examples of the food to which the mixed extract may be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, teas, and drinks. Alcoholic beverages and vitamin complexes.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are only for illustrating the present invention, and do not limit the content of the present invention.

Example 1 Preparation of Mixed Herbal Extracts

1.5 kg of the mixed herbal medicine powder, which were mixed in the ratio of 4: 4: 4: 1, was added to 3 l of an aqueous 70% ethanol solution and extracted at room temperature for 2 days. After 2 days, the solution was filtered through filter paper, and this process was repeated three times. The filtrate was concentrated under reduced pressure to remove ethanol and then dried by freeze drying to obtain a mixed herbal extract.

<Example 2-Example 10> Preparation of the mixed herbal medicine extract 2-10

The present inventors prepared the mixed herbal medicine extracts 2 to 10 in the same manner as in Example 1 by mixing the herbal medicine as follows (Table 1).

Example extract Herbal medicine Weight ratio Example 2 Extract 2 Upper lobe Exclusive Example 3 Extract 3 Medicinal beans Exclusive Example 4 Extract 4 Wolfberry Exclusive Example 5 Extract 5 Lettuce + medicinal beans 1: 1 Example 6 Extract 6 Upper leaf + goji berry 1: 1 Example 7 Extract 7 Medicinal beans + goji berry 1: 1 Example 8 Extract 8 Wolfberry + Antler 4: 1 Example 9 Extract 9 Lettuce + Yak + Deer Antler 8: 8: 1 Example 10 Extract 10 Upper leaf + goji berry + medicinal 1: 1: 1

Experimental Example 1 Animal Experiment in Osteoporosis Model Animal Experiment in Ovariectomized Rats

The present inventors performed an ovarian extraction experiment in females of SD (Sprague-Dawley) rats in which type I osteoporosis occurs after menopause, in order to conduct animal experiments on osteoporosis.

<1-1> Ovarian Extraction and Drug Administration

Sprague Dawley rats weighing 200-300 g at 10 weeks of age were used to perform bilateral ovarian extraction in all rat females of the control and test groups except the sham group. The Siamese group is a group that has performed all operations except ovarian extraction. The control group is an ovary-extracted and no drug-administered group. Administered group.

Specifically, ovarian extraction was performed with general anesthesia by intramuscular injection of 5 mg / 100 g of ketamine (Yangyang, Korea) and 1 mg / 100 g of xylazine (Bayer Korea, Korea) into the femoral muscles of rats. The lower abdomen was dissected around the midline, and the left and right ovaries were extracted and 0.1 ml of antibiotics (Sulpaporte-4, Unichem Co., Korea) were injected into the abdominal cavity to prevent infection.

After one week of recovery from the ovaries, the Siamese group and the control group received oral drinking water, and the E2 group was intraperitoneally injected with 17β-estradiol (Sigma-Aldrich) at 1 µg / kg / day. Thereafter, the mixed herbal medicine extract of the present invention obtained in <Example 1> was orally administered for 9 weeks at 5 g / kg / day as a dry medicinal equivalent.

<1-2> Measurement of Changes in Weight and Tissue Weight by Drug Administration

After the ovary was extracted by the above method, the weight of the experimental animals administered with the mixed herbal medicine extract was measured every week, and the tissue weight was measured after a certain period of time to determine the weight and tissue weight of the Siamese group and the control group by boneferroni multiple comparison. method).

The weight change of the animal is shown in Figure 1, the weight of the uterine tissue is shown in Figure 2. As shown in FIG. 1, the control group showed a sharp increase in body weight compared to the Siamese group, and the group of 5 g / kg / day of the mixed herbal medicine extract showed similar weight gain as the control group. The control group (E2) showed moderate weight gain between the Siamese group and the control group.

In addition, as shown in Figure 2, the weight of the uterus was sharply reduced in the control group compared to the Siamese group (normal group), the E2 administration group was restored to more than half of the normal group. The mixed extract 5g / kg / day administration group was not different from the control group.

Therefore, the mixed herbal medicine extract of the present invention does not change the body weight and the weight of the uterus compared to the control and control drug group is judged to be safe without changing the hormone in the body.

<1-3> Morphometric Analysis of Soju Bone Area

Morphometric analysis was performed in the lumbar and tibia of each group by the following method. Using a quantitative image analyzer (Quantitative image analysis system, Wild Leitz Co.) digitizer to automatically calculate the area of each shochu in the lumbar spine and tibia, which is the proximal part of the growth plate at the proximal end of each tibia. The area of trabecula in the inside of a rectangle having a reference area of 2 × 10 ⁶ μm ² was measured at a length of about 2/3 of the length of the growth plate.

As a result, as shown in Figure 3, the control group extracted from the ovary compared to the Siamese group (normal group) it can be seen that the osteoporosis is reduced by 45% of the area of the small bones after administration of E2 or the mixed herbal medicine extract of the present invention Increased the area of osseous bone (P <0.05) In the mixed administration group (Extract 1), the small bone area of the tibial bone increased to 155% of the control group, which had a similar effect to that of the E2 group.

Tibial Small Injury Area Lumbar bone minor bone area Control 100.0 ± 13.2 100.0 ± 5.4 Siamese 237.5 ± 10.7 116.4 ± 5.9 E2 group 154.5 ± 15.0 115.0 ± 5.1 Extract 1 154.6 ± 11.0 119.5 ± 2.1 Extract 2 129.3 ± 10.3 111.0 ± 3.6 Extract 3 123.2 ± 12.5 110.1 ± 2.3 Extract 4 121.0 ± 11.1 109.4 ± 3.7 Extract 5 138.8 ± 14.0 113.4 ± 3.7 Extract 6 134.5 ± 13.5 112.5 ± 5.9 Extract 7 131.2 ± 12.2 111.2 ± 3.0 Extract 8 141.8 ± 13.5 114.7 ± 3.1 Extract 9 145.8 ± 13.2 115.1 ± 3.2 Extract 10 149.1 ± 14.1 117.8 ± 2.5

Experimental Example 2 Toxicity Test and Histopathological Observation

Rats were dosed at 5 g / kg / day for 9 weeks and necropsied. Liver, kidney, lumbar and tibial tissues were collected in 10% formalin solution and demineralized in formic acid. The observation area of the tissue was cut with a surgical knife, and then dehydrated with xylene through a stepwise dehydration process ranging from 70 to 100% alcohol and acetone. Paraffin-embedded bone tissue was cut to 5 μm with a microtome, stained with hematoxylin and eosin (H & E), and observed under an optical microscope. There were no clinical symptoms or deaths in all animals treated with the test substance. Weight changes, blood tests, blood biochemistry, necropsy findings, and pathological differences between the groups were not observed. There was no in vivo toxicity due to administration.

Preparation Example 1 Preparation of Soft Capsule

Mixed herbal extracts prepared according to Example 1 100.0 mg, soybean oil 175.0 mg, lead 45.0 mg, coconut oil 127.5 mg, soybean phospholipid 21.0 mg, gelatin 212.0 mg, glycerin (specific gravity 1.24) 50.0 mg, di-sorbitol 76.0 mg, para 0.54 mg of oxycyanic acid methyl, 0.90 mg of paraoxybenzoic acid propyl, 0.56 mg of methyl vanillin, and yellow No. 203 were prepared according to the method of preparing soft capsules in the Pharmacopoeia General Regulations so that an appropriate amount of ingredients was contained in one capsule.

Preparation Example 2 Preparation of Tablet

Mixed herbal medicine extract prepared according to Example 1 100.0mg, corn starch 90.0mg, lactose 175.0mg, L- hydroxypropyl cellulose 15.0mg, polyvinylpyrrolidone 90 5.0mg and ethanol appropriately mixed raw material wet Granulation was carried out by granulation, and 1.8 mg of magnesium stearate was added and mixed, and the tablet was compressed to 400 mg.

Preparation Example 3 Preparation of Capsule

100.0 mg of the mixed herbal extract prepared according to Example 1, 83.2 mg of corn starch, 175.0 mg of lactose and 1.8 mg of magnesium stearate were homogeneously mixed and filled to contain 360 mg in one capsule.

Preparation Example 4 Preparation of Drinks

The present inventors prepared a beverage composition containing the mixed herbal medicine extract as an active ingredient as follows.

Mixed herbal medicine extract prepared according to Example 1 0.48 to 1.28 mg, honey 522 mg, thioctoamide amide 5 mg, nicotinic acid amide 10 mg, riboflavin sodium 3 mg, pyridoxine hydrochloride 2 mg, inositol 30 mg, ortho acid 50 mg And 200 ml of water in a composition and content to prepare a beverage using conventional methods.

The mixed herbal extracts of the upper lobe, goji berry, medicinal beans and antler of the present invention and the composition comprising the same can be used as a substitute for the existing bone disease prevention and treatment agent which has the side effect of preventing and treating other bone diseases such as osteoporosis, fracture, It can also be used as a health food.

Claims (8)

Osteoporosis prevention and treatment composition comprising the extract of the mixed herbal medicine mixed with 4 parts by weight of upper leaves, 4 parts by weight of wolfberry, 4 parts by weight of medicinal beans and 1 part by weight of antler. delete delete The composition of claim 1 wherein the solvent for extraction is water, C ₁ to C ₃ lower alcohols or mixtures thereof. The composition of claim 4, wherein the alcohol is ethanol. The composition of claim 1 wherein the solvent for extraction is 5 to 95% water and 95 to 5% alcohol. delete Health food for osteoporosis prevention and improvement comprising the extract of the mixed herbal medicines mixed with 4 parts by weight of upper leaves, 4 parts by weight of wolfberry, 4 parts by weight of medicinal beans and 1 part by weight of antler.

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