KR100715631B1 - Production method of isoflavone enforced eggs - Google Patents
Production method of isoflavone enforced eggs Download PDFInfo
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- KR100715631B1 KR100715631B1 KR1020050132852A KR20050132852A KR100715631B1 KR 100715631 B1 KR100715631 B1 KR 100715631B1 KR 1020050132852 A KR1020050132852 A KR 1020050132852A KR 20050132852 A KR20050132852 A KR 20050132852A KR 100715631 B1 KR100715631 B1 KR 100715631B1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L15/00—Egg products; Preparation or treatment thereof
- A23L15/20—Addition of proteins, e.g. hydrolysates, fats, carbohydrates, natural plant hydrocolloids; Addition of animal or vegetable substances containing proteins, fats, or carbohydrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L15/00—Egg products; Preparation or treatment thereof
Abstract
본 발명은 대두 이소플라본의 일종인 genistein 혹은 daidzein을 계란에 전이시키는 방법으로, 지용성 물질인 이소플라본을 피하에 이식하여 체액에 서서히 용해되어 혈액으로 유입되도록 한 후 계란에 전이, 축적되도록 유도하는 이소플라본 강화 기능성 계란생산방법에 관한 것이다. 본 계란생산방법은 순도 97% 이상의 Genistein 혼합물을 준비하는 단계와, 압출기를 사용하여 상기 혼합물 250mg을 펠렛으로 성형하는 단계와, 성형된 500mg, 1g 중 어느 하나의 용량으로 각각 복수개를 산란계의 어깨부위 피하에 이식시키는 단계를 포함한다.The present invention is a method of transferring genistein or daidzein, a type of soy isoflavone, to an egg. The isoflavone, which is a fat-soluble substance, is implanted subcutaneously, so that it is gradually dissolved in body fluids to be introduced into the blood, and then is induced to accumulate and accumulate in eggs. The present invention relates to a method for producing flavone-reinforced functional eggs. The egg production method comprises the steps of preparing a Genistein mixture with a purity of 97% or more, molding 250 mg of the mixture into pellets using an extruder, and forming a plurality of shoulder portions of the laying hens in any one of 500 mg and 1 g. Implanting subcutaneously.
이소플라본, genistein, daidzein, 기능성 축산물, 계란, 전이기술 Isoflavones, genistein, daidzein, functional livestock, eggs, transfer technology
Description
본 발명은 이소플라본 강화 기능성 계란 생산방법에 관한 것으로, 보다 상세하게는 대두 이소플라본의 일종인 genistein 혹은 daidzein을 계란에 전이시키는 방법으로, 지용성 물질인 이소플라본을 피하에 이식하여 체액에 서서히 용해되어 혈액으로 유입되도록 한 후 계란에 전이, 축적되도록 유도하는 이소플라본 강화 기능성 계란 생산방법에 관한 것이다.The present invention relates to a method for producing isoflavone-reinforced functional eggs, and more particularly, to genistein or daidzein, which is a type of soy isoflavone, is transferred to eggs, and is slowly dissolved in body fluids by implanting isoflavone, a fat-soluble substance, subcutaneously. The present invention relates to a method for producing isoflavone-reinforced functional eggs which induces blood to be transferred and accumulated in eggs.
종래 기개발 유사 특허로는 일본에서 Matsude 등이 Genistein 혹은 Daidzein 함유량이 높은 계란 혹은 계란생산방법이 있다. 기존의 이소플라본 강화 계란 생산방법은 정제된 이소플라본 혹은 이소플라본이 함유된 대두 부산물을 가금사료에 첨가급여하고 체내 유입된 이소플라본은 소화기관에서 일부 분으로 배설되고 일부 혈액으로 흡수되어 계란의 난황에 축적, 전이시키는 방법이었다(Takita 등, 2001; Matsuda 등, 2004). 따라서 일반 사료와 혼합되는 과정과 소화되는 과정중 분해되거나 분으로 유실되는 부분이 있어, 사료첨가형 전이기술은 체내 혈액으로 직접 유입되는 부분이 상실되는 등 계란내 축적되는 이소플라본의 총량을 감소시키는 경향 이 있다. Previously developed similar patents in Japan include Matsude et al. Egg or egg production method with high Genistein or Daidzein content. Existing method for producing isoflavone fortified egg is to add purified isoflavone or soybean by-product containing isoflavone to poultry feed, and isoflavone introduced into the body is excreted as part of the digestive tract and absorbed into some blood to make egg yolk Was accumulated and transferred (Takita et al., 2001; Matsuda et al., 2004). Therefore, during the mixing and digestion process with the general feed and digestion, there is a part that is broken down or lost, feed-transfer transfer technology tends to reduce the total amount of isoflavones accumulated in eggs, such as the loss of the portion that directly enters the body blood There is this.
그러나, 이와 같은 종래의 방법은 단순히 고농도의 이소플라본을 사료첨가제로서 급여하여 계란내의 함량을 높이는 방법으로 가축에게 이소플라본 강화 계란을 생산하고자 하는 기간까지 장기간 지속 및 반복적으로 급여해야하므로 고가의 고순도 정제 이소플라본 가격을 고려해 볼 때 경제적인 면에서 실효성에 단점이 있다.However, such a conventional method simply by feeding a high concentration of isoflavones as a feed additive to increase the content of eggs in the animal to be fed a long-term continuous and repeatedly until the period to produce isoflavone fortified eggs to livestock, expensive high-purity purification Considering the price of isoflavones, there are disadvantages in terms of effectiveness.
본 발명은 상기와 같은 문제점을 해결하기 위해 안출한 것으로, 대두 이소플라본의 일종인 genistein 혹은 daidzein을 계란에 전이시키는 방법으로, 지용성 물질인 이소플라본을 피하에 이식하여 체액에 서서히 용해되어 혈액으로 유입되도록 한 후 계란에 전이, 축적되도록 유도하는 이소플라본 강화 기능성 계란 생산방법을 제공하는데, 그 목적이 있다.The present invention has been made to solve the above problems, a method of transferring genistein or daidzein, which is a type of soy isoflavone to eggs, is implanted into the blood by subcutaneous transplant of fat-soluble isoflavones into the body fluid into the blood To provide a method for producing isoflavone-reinforced functional eggs to induce transfer and accumulation in the egg, so that the object is.
상기 목적은, 본 발명에 따라, 순도 97% 이상의 Genistein 혼합물을 준비하는 단계와, 압출기를 사용하여 상기 혼합물 250mg을 펠렛으로 성형하는 단계와, 성형된 500mg, 1g 중 어느 하나의 용량으로 각각 복수개를 산란계의 어깨부위 피하에 이식시키는 단계를 포함하는 이소플라본 강화 기능성 계란 생산방법에 의해 달성된다.The object of the present invention is to prepare a Genistein mixture with a purity of 97% or more, molding 250 mg of the mixture into pellets using an extruder, and forming a plurality of 500 mg and 1 g of each one of the plurality. It is achieved by a method for producing isoflavone-reinforced functional eggs comprising implanting subcutaneously under the shoulder of a laying hen.
이하, 본 발명에 따른 이소플라본 강화 기능성 계란 생산방법을 상세하게 설명하면 다음과 같다.Hereinafter, described in detail the method for producing isoflavone-reinforced functional egg according to the present invention.
위에서 언급한 바와 같이 종래의 일반적인 방법에 비해 본 발명은 정제된 이소플라본을 피하에 이식시켜 직접적으로 혈액으로 유입시키므로 소화과정에서 손실이 없고, 서방형으로 혈중 호르몬 농도를 일정하게 유지하여 장기간 최적 및 최고 수준으로 손실없이 계란에 이소플라본을 전이, 축적시킬 수 있는 것이다.As mentioned above, the present invention has no loss in the digestion process because it is directly injected into the blood by implanting purified isoflavones subcutaneously, and maintains the constant hormone concentration in the sustained-release mode for long-term optimal and At the highest level, isoflavones can be transferred and accumulated in eggs without loss.
또한, 본 방법은 목적물질이 생리활성을 가진 기능성 천연물질, genistein을 가축에게 장착하여 혈액을 통해 계란과 같은 축산물에 최고와 최적 수준으로 직접 전이시켜 부가가치가 향상된 축산물을 생산할 수 있다.In addition, this method can produce livestock products with improved value added by directly attaching to the livestock, a functional natural material, genistein, which has a biologically active target, to the livestock, such as eggs, at the highest and optimal levels.
이와 같은 기능성 계란 생산방법은 압출기를 사용하여 순도 97% 이상의 Genistein 혼합물 250mg이 펠렛으로 성형된 후 500mg, 및 1g의 용량으로 각각 2개, 4개씩 산란계의 어깨부위 피하에 이식됨으로 이소플라본이 축적된 계란을 생산할 수 있다. 즉, 피하에 이식된 Genistein은 산란계의 혈액으로 유입되어 식물성 이소플라본으로 존재하여 소화과정에서 손실없는 영양성분을 그대로 간직하므로 이소플라본이 축적된 계란을 생산한다.This functional egg production method is a 250mg of Genistein mixture with a purity of 97% or more using an extruder is molded into pellets and then implanted into the shoulder of the laying hens 2, 4 at the dose of 500mg and 1g, respectively, to accumulate isoflavones. You can produce eggs. That is, Genistein implanted subcutaneously flows into the laying hen's blood and is present as vegetable isoflavones, which retains nutrients that are not lost during digestion, producing eggs with isoflavones accumulated.
이소플라본 이식 장치를 산란계에 장착 후 계란의 난황내에서 검출되는 genistein의 함량을 HPLC 및 HPLC-MS을 통해 분석, 이소플라본이 고농도로 함유된 기능성 계란임을 확인할 수 있다.After mounting the isoflavone transplanter on the laying hen, the content of genistein detected in egg yolk is analyzed by HPLC and HPLC-MS, and it is confirmed that the isoflavone is a functional egg containing a high concentration.
이와 같이 계란에 이식된 genistein의 축적함량의 변화는 다음과 같다.Changes in the accumulation of genistein implanted in eggs are as follows.
1000mg genistein을 본 발명의 전이방법으로 실시하고 시간에 따른 계란내 genistein의 전이율과 함량을 genistein을 전이하지 않은 구(대조구)와 비교했을 때 결과는When the 1000 mg genistein was carried out by the transfer method of the present invention and the transfer rate and content of genistein in eggs with time were not compared with those without control of genistein (control)
임을 알 수 있다.It can be seen that.
본 전이방법을 이용했을 때 계란내 genistein의 전이, 축적효율은 아래 표와 같이 Genistein 1000 mg을 피하에 이식하였을 경우 이식 13일 이후에는 218ng/g(난황)의 genistein이 난황에서 검출되고, 이식 후 30일 째의 경우에는 514ng/g의 genistein이 난황에서 검출되며, 유사한 농도가 50일 이상 지속됨을 알 수 있다. The transfer and accumulation efficiency of genistein in egg using this method is as follows. When 1000 mg of Genistein is subcutaneously implanted, 218 ng / g of genistein is detected in egg yolk after 13 days of transplantation. At day 30, 514 ng / g genistein was detected in egg yolk and similar concentrations persisted for more than 50 days.
같은 방법으로 Genistein 500 mg을 피하에 이식하였을 경우 계란내 genistein의 전이, 축적효율은 아래 표와 같이 Genistein 500mg을 이식하였을 경우, 18일째에는 395ng/g의 genistein이 난황에서 검출되고, 60일 이후에는 595ng/g의 genistein이 난황에서 검출되어 계속적으로 농도가 유지됨을 알 수 있다.In the same way, when genistein 500 mg was implanted subcutaneously, the transfer and accumulation efficiency of genistein in eggs was measured as shown in the table below. When Genistein 500 mg was implanted, 395 ng / g genistein was detected in egg yolk on day 18 and after 60 days 595 ng / g genistein was detected in egg yolk and the concentration was continuously maintained.
본 발명의 실시와 기존의 Matsuda 방법의 축적효율을 비교하면 다음과 같다.Comparing the accumulation efficiency of the practice of the present invention and the existing Matsuda method is as follows.
위 표와 같이, 종래의 방법(Matsuda 등, 2004)에 의하면 0.28 mg/사료g과 0.84 mg/사료g의 genistein을 급여하였을 경우 계란내 이소플라빈이 각 각 136 ng/난황g, 272 ng/난황g 검출된다.As shown in the table above, according to the conventional method (Matsuda et al., 2004), when the genistein of 0.28 mg / g and 0.84 mg / g is fed, isoflavin in eggs is 136 ng / yg and 272 ng / y, respectively. g is detected.
그러나, 본 개발 전이방법의 경우에는 500 mg genistein을 1회 이식하였을 경우 500 ng/난황g의 genistein이 함유된 계란을 50일 이상 지속적으로 생산할 수 있다. However, in this developmental transfer method, a single 500 mg genistein transplantation can produce eggs containing 500 ng / yg of egg yolk genistein for more than 50 days.
따라서, 종래의 방법은 272 ng/난황g의 genistein이 함유된 계란을 50일 동안 생산하기 위해서 기존의 방식은 100mg의 genistein을 사료를 통해 지속적으로 50일간 공급해야 한다.Therefore, the conventional method requires 50 days of continuous feeding of 100 mg of genistein through the feed for 50 days to produce eggs containing 272 ng / g of egg yolk genistein for 50 days.
이상의 결과를 비교해 볼 때, 본 발명이 기존의 사료 첨가형 급여방법에 비해 난황내 이소플라빈의 축적함량이나 이소플라빈 공급량에 따른 전이효율 면에서, 약 20배 이상 효과적으로 이소플라빈 강화 기능성 계란을 생산할 수 있는 것으로 나타났다. 또한, 이러한 방법은 동물의 생리적 특성상 모든 phytoestrogen을 비롯한 생리활성물질에 공히 적용될 수 있는 방법이라고 할 수 있다.Comparing the above results, the present invention is about 20 times more effective in terms of transfer efficiency according to the accumulation content of isoflavin or isoflavin supply in egg yolk compared to the conventional feeding method. It can be produced. In addition, this method can be said to be applicable to all phytoestrogen, including phytoestrogen due to the physiological characteristics of the animal.
본 발명은 순수 정제된 이소플라본을 가축의 피하에 이식하여 혈중 이소플라본의 함량을 극대화하여 가장 효율적이고 경제적으로 계란내 전이, 축적시키기 때문에 부가가치가 향상된 축산물을 생산할 수 있다. The present invention can maximize the content of isoflavones in the blood by grafting purely purified isoflavones to subcutaneous livestock, thereby producing livestock products with improved added value because they are most efficiently and economically transferred and accumulated in eggs.
또한, 궁극적으로는 본 방법을 통해 이소플라본이 강화된 기능성 계란을 생산하기 때문에 계란으로부터 실물성 영양분을 충분히 섭취할 수 있는 효과가 있다.In addition, ultimately, since the method produces an isoflavone-enriched functional egg, there is an effect of sufficiently ingesting nutrients from the eggs.
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JP2002165582A (en) | 2000-11-29 | 2002-06-11 | Natl Fedelation Of Agricult Coop Assoc | Isoflavone-containing egg, method for producing the same and feed material to be used for producing isoflavone- containing egg |
JP2003310177A (en) | 2002-04-25 | 2003-11-05 | New Food Creation Gijutsu Kenkyu Kumiai | Egg having high estrogen like activity, method for producing the same and feed raw material used for the same method |
JP2003330707A (en) | 2002-05-09 | 2003-11-21 | Mitsubishi Electric Corp | Pipe line processing circuit and its designing method |
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KR101024394B1 (en) * | 2008-02-29 | 2011-03-23 | 한국방송통신대학교 산학협력단 | A method for improving quantity of accumulated fat in animal musculartissue using subcutaneous implantation of soybean isoflavone genestein substance |
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