KR100686258B1 - New turfgrass cultivar of Kumsan Poa pratensis L. - Google Patents

Abstract

The present invention relates to a new breed of "Gumsan" Poa pratensis L. of the native native Kingtucky bluegrass (English name: Kentucky bluegrass), more specifically, the blue period is about 10 months, the green color is dark green, , Multi-proliferative mid lobe dwarf, herb length of 9 + 0.5㎝, leaf length of 8.5 + 0.5㎝, leaf width of 2.5 + 0.1㎜, withdrawal length of 38 + 5㎝, yield of 6 points, medium ripeness It is for the "geumsan" Wangpoa grass, which is capable of asexually repeat reproduction with morphological characteristics.

“Geumsan” Wangpoa Grass of the present invention has a longer green period than Korean grasses such as field grass or gold grass, so that it is possible to produce a blue landscape in all seasons except cold weather, and it is vigorous and rich in color, and after the creation of a lawn It has the morphological characteristics that can make excellent grass surface due to its high density among individuals, and has been adapted to the Korean environment for a long time, so it has excellent advantages such as disease resistance, dry resistance, and resistance against introduced species.

New varieties of grass, Wangpoa grass, Geumsan

Description

New Grass Geumsan Wangpoa Grass {New turfgrass cultivar of Kumsan Poa pratensis L.}             

1 is a photograph of the "geumsan" Wangpoa grass of the present invention.

Figure 2 is an electrophoresis result showing the identification results of RAPD markers of "Geumsan" Wangpo Azandi compared to the 6 varieties of the domestic collection species Wangpo Azandi, including the midnight (Midnight) introduced. Lane 1 is P77, lane 2 is P38, lane 3 is Unique, lane 4 is Blue star, lane 5 is Challenger, lane 6 is Midnight, lane 7 is 00034, lane 8 is 01094, lane 9 is 02062, Lane 10 is Geumsan, Lane 11 is Award, Lane 12 is New Port, Lane 13 is Prickness and Lane 14 shows the results of PCR of Monopoly.

Fig. 3 is a dendrogram showing the relationship between native and introduced Wangpoa grass. P77, P38, 00034, 01094, 02062 and Geumsan are the domestic collection Wangpoa grass, and Unique, Blue star, Challenger, Midnight, Award, New Port, Prickness and Monopoly are the introduction Wangpoa grass.

The present invention relates to a new breed of "Gumsan" Poa pratensis L. of the native native Kingtucky bluegrass (English name: Kentucky bluegrass), more specifically, the blue period is about 10 months, the green color is dark green, , Multi-proliferative mid lobe dwarf, herb length of 9 + 0.5㎝, leaf length of 8.5 + 0.5㎝, leaf width of 2.5 + 0.1㎜, withdrawal length of 38 + 5㎝, yield of 6 points, medium ripeness It is for the "geumsan" Wangpoa grass, which is capable of asexually repeat reproduction with morphological characteristics.

Poa spp. Plants in Korea are Poa pratensis L., Poa nipponica Koidz., Poa annua L., Poa compressa L. 18 species, including grass ( Poa radula Fr. et Sav.) Grow wild, and in North Korea there are 14 species including 4 species that have not been reported in Korea, including Poa hisauchii Honda and Poa komarovii roshev. Distributed.

Poa pratensis L. is also called kingweed grass (North Korean grasshopper). It is a perennial herb that commonly grows on riversides, hills, fields and roadsides in Korea. It is widely known and is the most representative Korean-style grass that is most used in parks, gardens, golf courses, stadiums and slopes in temperate and boreal regions.

Korean grass is a perennial herbaceous plant, belongs to the flower family, and reproduces with crawling and underground diameters, and shows many variations in form and characteristics depending on the region. Until now, according to the research, five to six kinds of grass ( Zoysia. Japonica ), gold grass ( Z. matrella ), birod grass ( Z. tenufolia ), seagrass ( Z. sinica ), and king grass ( Z. macrostachya ) Grass has been reported to be distributed, but since it has been commonly found in grassy soils ( Z. japonica Steud.) That grow well in poor soil and withstand pests as well as dry and cold resistance, grass has been called ritual field grass since ancient times. Thus, the awareness of other grass species was low. However, the grass has the disadvantage of low coverage density, dark color, slow germination and composition rate, and especially blue color for only about 6 months of the year.

On the other hand, wangpoa grass usually lasts more than 10 months to produce a blue landscape year-round, and the composition rate is fast, it is useful for early greening, and has the characteristics of growing well in the shade or wetlands.

Korean grass is the most suitable grass in Korea's climate, and it is more environmentally adaptable than other cold grass and other warm grass. In particular, it is known to be strong in disease resistance, dry resistance, and resistance to corrosion. However, Korean turf has a fatal disadvantage of short green period, and all of the turf currently used in Korea is foreign imported species.

However, in case of foreign introduced species, it is not a kind adapted to Korea's climatic characteristics, and there are various problems such as rapid decline in growth if careful management is not followed after grass formation.

Therefore, in the art, based on the advantages of the above-mentioned Korean grasses, breeding of excellent varieties with the best characteristics to replace imported species by overcoming the shortcomings of the shortest green period and the shortest green period are urgently required. have.

Therefore, the present inventors have continuously compared and observed the morphological and cultivated aspects of native wangpoa pool and introduced species collected from all over Korea, and among them, “Geumsan” wangpoa grass has color compared to other native species and introduced species. Not only has excellent grassy properties such as grass, grass, and texture, but also has strong growth, high color and high density after individual grass formation, and not only has morphological characteristics that can make excellent grass surface, but also disease resistance and dry resistance. The characteristics were also good and it was found that this had the optimal characteristics to replace the existing imported species.

Therefore, the object of the present invention is about 10 months blue period, dark green color, multi-probability mesenchymal formation, grass length is 9 + 0.5cm, leaf length is 8.5 + 0.5cm, leaf width is 2.5 + 0.1 ㎜, withdrawal length is 38 + 5cm, withdrawal amount is 6 points, and maturity is medium. Poa pratensis L. ) To provide.

In order to achieve the above object, the present invention has a blue period of about 10 months, the leaf is dark green, multiply mesodermal dwarf formation, the length of the grass is 9 + 0.5cm, the length of the leaf is 8.5 + 0.5cm, leaf width 2.5 + 0.1㎜, withdrawal length 38 + 5㎝, withdrawal amount is 6 points, the maturity is medium morphological characteristics, A, E, F and OPB-5 primer of OPB-4 primer (manufactured by Operon) A of (made by operon), B, C, G, I of OPB-7 primer (made by operon company), A of OPB-8 primer (made by operon company), E of OPB-10 primer (made by operon company), A and B bands of the OPB-18 primer (operon company) do not appear, D, H of the OPB-7 primer, B, D of the OPB-8 primer, F of the OPB-10 primer, E band of the OPB-18 primer Poa pratensis L., which is characterized by the appearance of "geumsan" wangpoa grass, is provided.

Hereinafter, the present invention will be described in detail.

In the field of Korea, various wangpoa grasses grow as native plants, and these are ecological species that are highly adapted to Korean climatic conditions.They select high-quality species through commercial collection, selection and breeding and replace them with commercially developed species. can do.

The present inventors classified 380 strains of wangpoa pools collected through collection activities from 2000 to the present by morphological, cultivative and genetic characteristics, ie, DNA fingerprinting, and then analyzed the genomics of “Geumsan” wangpoa grass (Kentucky Bluegrass). The genetic relationship between the genus Poa and the taxonomic location of the collected and introduced species in Korea were identified, and this was bred as a new breed of Wangpoa grass.

The “Geumsan” Wangpoa Grass selected by the inventors has a blue period of more than 10 months, and the blue period is longer than that of Korean grasses such as field grass or gold grass, so that it is possible to produce blue landscapes in all seasons except cold weather, and the color is dark. It has the characteristics of high surface coverage after composition and high density between individuals due to its spreading sideways, and has been adapted to Korea's environment for a long time and has excellent advantages such as disease resistance, dry resistance and resistance against introduced species.

The present inventors compared and analyzed the lawns collected from the roadside of Sincheon 1-ri, Namil-myeon, Geumsan-gun, Chungcheongnam-do using RAPD (Random Amplified Polymorphic DNA) analysis method (DNA fingerprinting) to distinguish “Geumsan” Wangpoa grass from other native species and introduced species. The present invention confirmed the existence of biochemical markers that can be used, and also revealed the relation of genetic spatula in Poa to provide information on varieties of “Geumsan” Wangpoa grass, and found that it is possible to distinguish them from other systems and varieties. Came to complete.

According to the results of the RAPD analysis, “Geumsan” Wangpoagrass is different from other Wangpoagrass in A, E, F of OPB-4 primer and A of OPB-5 primer, and B, C, G, I of OPB-7 primer. , A of OPB-8 primer, E of OPB-10 primer, A, B of OPB-18 did not show band, D, H of OPB-7 primer, B, D, OPB-10 of OPB-8 primer In the F of the primer, E of the OPB-18 showed a band appearing properties it can be used as a data for identifying the grass.

The polymorphic bands obtained from the six primers were synthesized and statistically analyzed. As a result of the dendogram of FIG. 3, Wangpoa grass was divided into three groups at the SED (Squared Euclidean Distance) 20 level, and 'Geumsan' Wangpoa grass was introduced. They were classified in the same group as the species Award, Challenger, Monopoly, Prickness, Unique and native species 00034, 01094 and 02062. "Geumsan" Wangpoagrass has similarities to other strains within Group I. Based on the RAPD analysis, it was confirmed that 'Geumsan' Wangpoa Grassy is genetically distinct from the introduced breed and the autogenous collection species.

Wangpoa grass of the present invention is a well-developed rhizome (rhizome) is a plant that can be easily propagated a sideways propagation by propagation, trophic breeding through artificial breeding or plugging. In addition, seed propagation in addition to trophic propagation, unlike other species, the seed generation is a phenomenon that is formed without modification is very high, and also can be repeatedly reproduced by tissue culture well known in the art. It will be apparent to those skilled in the art.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1: Harvesting Grass

The present inventors planted “Geumsan” Wangpoagrass, collected on March 7, 2003, at Sincheon 1-ri, Namil-myeon, Geumsan-gun, Chungcheongnam-do, Korea, at the Sewon Botanical Research Institute farm in Nonsan, Chungnam, Korea. DNA fingerprint analysis was performed.

Example 2: Morphological Characterization

In order to understand the morphological characteristics of the collected Wangpoa grass, the height, length, width of the leaves, the length of the leaves, the amount of water and the maturity were measured by the Sewon Botanical Research Institute on April 24, May 31, and June 7, 2003. Measured on the farm.

The height is the maximum height of Wangpoa grass, the length of the leaf is the length of the second leaf, the width of the leaf is the maximum width of the second leaf, the length of the exit diameter is the length of the stem excluding the ear at the time of extraction, According to the maturity of 1-9 points, the maturation status of the harvested seeds was assessed by dividing them into preterm, rebirth, and late life. Morphological and physiological characteristics of the collected grasses are shown in Table 1 below.

From the results of Table 1, "Geumsan" Wangpoa grass was 9cm long, 8.5cm long, 2.5mm wide, withdrawal length 38cm, water output 6 points, and medium ripening. The herb length was 9㎝ and belonged to dwarf molding, and the leaf length was short as 8.5㎝. The leaf width was 2.5mm, which was close to the mesenchymal shape, and the length of the exit diameter was 38cm, which was very short, but the seeds run relatively much. However, maturity was moderate.

Example 3: Investigation of germination, germination and visible coverage

In order to evaluate the germination, germination, and visible coverage of native Wangpoagrass, two seeds from 16 species of domestically collected species, including “Geumsan” Wangpoagrass, were seeded in a plug seedling box on September 6, 2003. After germination, germination and visible coverage were investigated, the results are shown in Table 2.

As a result of the evaluation, the germination, germination and visible coverage of the collected and introduced species showed a lot of difference. In the case of “Gumsan” Wangpoagrass, the germination and germination rate were poor, but the initial germination rate was shown on April 17, 2004. Compared with the good coverage.

Example 4 Growth Characteristics

In order to understand the growth characteristics of the "Geumsan" Wangpoa turf, 16 kinds of domestically collected and 2 introduced species were harvested in September 2003, including "Geumsan" Wangpoagrass, which showed the excellent characteristics of the Wangpoa turf. After sowing in the plug box on the 6th, the test packaging was prepared on the 24th of April 2004 at the Sewon Plant Research Institute Nonsan Farm using the produced plug seedlings. After sowing, management tasks such as watering, fertilization and cutting were carried out conventionally.

The results of evaluating the visible quality of the collected Wangpoa grass from five times from July 31, 2004 to December 12, 2004 are shown in Table 3. Wangpoa grass of “Geumsan” showed superior growth characteristics compared to other collected species, and the average value was found when comparing the mean value with the introduced species Midnight.

The highest growth was observed on July 31, the summer season, and the highest on December 12, 2004, when it passed through autumn and winter, showing the best growth compared to other collections and introduced species until late winter. It was judged to maintain quality.

The results of evaluating the coverage, visible color evaluation and visible density of the collected Wangfoa grasses are shown in Table 4. Wangpoa grass, “Geumsan”, was relatively better in coverage and visible color than other collections. It was the highest in the visible density measured on October 2 and November 6, 2004, and was considered to have excellent turf characteristics.

Example 5: Classification of "Geumsan" Wangpoagrass using RAPD

To identify the taxonomic location of "Keumsan" Wangpo Agrass and to identify the relationship and sympathy between Wangpo Agrass, 6 types of domestic collections and 8 introduced species RAPD analysis was performed.

The primers used for RAPD were operonsa pra, and 6 types such as OPB-4, OPB-5, OPB-7, OPB-8, OPB-10, and OPB-18 were used, and the results of the RAPD profile are shown in FIG. 2. Figure 2 is an electrophoresis picture showing the results of RAPD assay of the introduced species and native collection Wangpoapool using the six primers, M shows the DNA size marker, lane 1 (lane 1) is P77, lane 2 is P38 , Lane 3 is Unique, Lane 4 is Blue star, Lane 5 is Challenger, Lane 6 is Midnight, Lane 7 is 00034, Lane 8 is 01094, Lane 9 is 02062, Lane 10 is Geumsan, Lane 11 is Award, Lane 12 is New Port, lane 13 shows Prickness, and lane 14 shows the PCR results of Monopoly.

A total of 43 polymorphic bands were obtained, and the band patterns were different between the Wangpoa grass introduced and native collection species in all random primers used in the experiment.

Polymorphic bands obtained from the RAPD assay using six primers were symbolized as shown in Table 5. Unlike other Wangpoa grasses, “Geumsan” Wangpoagrass has the A, E, F and OPB-5 primers of OPB-4 primers, and the B, C, G, I, OPB-8 primers of OPB-7 primers. A, E of OPB-10 primer, A, B of OPB-18 did not show band, D, H of OPB-7 primer, B, D of OPB-8 primer, F of OPB-10 primer, F of OPB-10 primer, OPB- In E of 18, the band appeared to be used as data for grass identification.

 FIG. 3 is a dendogram using a close matrix and a group linkage method in which the genetic distance between each variety was measured by SED (Squared Euclidean Distance) by synthesizing, statistically analyzing, and encoding polymorphic bands obtained from the six primers. As shown in the dendrogram of Figure 3 Wangpoa grass was divided into three groups at the SED (Squared Euclidean Distance) 20 level, "Gumsan" Wangpoa grass is introduced species Award, Challenger, Monopoly, Prickness, Unique and native species 00034, 01094, 02062, etc., were classified in the same group I. “Geumsan” Wangpoagrass has a similar degree to other strains within Group I. Based on the RAPD analysis, it was confirmed that 'Geumsan' Wangpoa Grassy is genetically distinct from the introduced varieties and native collection species.

Example 6 Asexual Breeding Method of "Geumsan" Wangpoa Grass

Zoysia grass is characterized by very strong creepiness and is propagated laterally by ground crawling and underground. Rhizomes are horizontal stems that extend horizontally, with each eye producing one eye, which is a shoot, a new shoot, and eventually the stem of the ground that produces the branches. Becomes At the same time, roots emerge from each node of the underground diameter. Stolons are horizontal stems on the ground that also have pedicles and roots at each node. A new kind of grass, Geumsan, a kind of Korean grass, has the ability to reproduce by underground or ground crawling, which forms a dense cushion-like model. Asexual breeding of the new grass species "Geumsan" of the present invention was carried out as in the following experimental example.

6-1: Propagation Method by Sprigging

Sprigs are parts of grassy plants that contain terrestrial or underground diameters. Sprigs of the new varieties of the grass "Geumsan" were cut to include nodes and planted by digging bones 3 to 5 cm deep at intervals of 10 to 20 cm. After planting, water was supplied and managed sufficiently, and grass and roots came out from each node.

6-2: How to multiply by Stolonizing

The ground crawling or underground view of the new grass species "Geumsan" was cut to include nodes, and the area was scattered to form grass forge. In order to prevent drying after midwife, watering was performed after surface covering. This method requires a larger amount of grass than the Sprigging.

6-3: How to multiply by Sodding

When planting wide and flat square grass turf with a length of 2 to 6 feet and a width of 1 to 1.5 feet at regular intervals, the empty space between the turf is filled by ground crawling and underground. lost.

6-4: How to multiply by Plugging

A plug is a small piece of cymbal, cylindrical or block shaped. When the grass plugs were planted at regular intervals, the space between the plugs was filled by the ground crawling and underground views, thereby creating a lawn.

6-5: How to Multiply Using Multishoot

After disintegrating tissues with strong divisions such as ground crawling or underground section, and fertilization, MS containing BA (6-benzyladenin) 0.1 ~ 1.0mg / L and NAA (1-naphthaleneacetic acid) 0.05 ~ 0.1mg / L (Murashige Skoog) was wounded in a medium to abandon the multishoot. The separated multishoots were separated into individual individuals and transferred to 1/2 concentration medium containing no hormones. The rooted plants were purified and grown in greenhouses.

6-6: regrowth of plants from callus to multiply

2,4-D (2,4-dichlorophenoxyacetic acid) after collecting and disinfecting active tissues such as nodules, immature inflorescences, immature embryos, fertilizations, and tips of roots in a new breed of grass "Gumsan". ) Callus was organically hydrated by 6-5 MS medium (Murashige Skoog) containing 1-5 mg / L and 0.5-1.0 mg / L kinetin. The separated callus was transferred to a 1/2 medium medium containing no hormone to regenerate the plant. The regenerated plants were purified and grown in greenhouses.

Having described the specific part of the present invention in detail, it is obvious to those skilled in the art that such a specific description is only a preferred embodiment, thereby not limiting the scope of the present invention. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

“Geumsan” Wangpoa Grass of the present invention has a longer green period than Korean grasses such as field grass or gold grass, so that it is possible to produce a blue landscape except for cold seasons, and has excellent grass properties such as grass height and texture, It has a morphological characteristic that can produce excellent grass surface because it has strong growth, dark color after grass formation, and high density between individuals after grass formation, and it has been adapted to the environment of Korea for a long time. Has excellent advantages such as pacing.                     

In addition, Wangpoa grasses and introduced species collected from all over Korea were compared using DNA fingerprinting method to select “Geumsan” Wangpoa grass specific DNA markers, thereby providing a foundation to distinguish them from other systems and varieties. It works.

Claims (1)

Blue period is about 10 months, leaf is dark green, multiply mesenchymal dwarf, herb length is 9 + 0.5cm, leaf length is 8.5 + 0.5cm, leaf width is 2.5 + 0.2mm, length is 38 + 5cm, withdrawal amount is 6 points, maturity has moderate morphological characteristics, A, E, F of OPB-4 primer (made by operon) and A, OPB- of OPB-5 primer (made by operon) B, C, G, I of 7 primer (made by operon), A of OPB-8 primer (made by operon), E of OPB-10 primer (made by operon), and OPB-18 primer (made by operon) “Gumsan” Wangpoa, characterized by no bands A, B, D, H of OPB-7 primers, B, D of OPB-8 primers, F of OPB-10 primers, and E bands of OPB-18 Poa pratensis L., a grassy, reproductive species named grass.

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