KR100419720B1 - New turfgrass cultivar of Pureundeul Poa pratensis L. - Google Patents

Abstract

The present invention relates to a new breed of 'Purples' ( Poa pratensis L.) of native Korean wild pooh grass (Kenttucky bluegrass). Wangpoa grass of the present invention has a longer green period than Korean grasses such as field grass or gold grass, so it is possible to produce blue landscapes in all seasons except cold weather, and the germination rate is fast and spreads well laterally. It has excellent characteristics such as high yield of seeds with high yield, and has been adapted to Korean environment for a long time.

Description

New turfgrass cultivar of Pureundeul Poa pratensis L.

The present invention relates to a new kind of "Purples" ( Poa pratensis L.) of domestic native Wangtua grass (English name: Kentucky bluegrass). More specifically, the grass collected from Yasan in Naeduk-dong, Sanggu-gu, Cheongju-si, Chungcheongbuk-do, Korea was sorted by DNA fingerprinting method. It is about identifying the taxonomic position with the fields and fostering them as new varieties of Wangpoa grass.

In Korea, Poa spp . Is a plant of Poa pratensis L., Poa nipponica Koidz., Poa annua L., Poa compressa L., 18 species, including grass ( Poa radula Fr. et Sav.) Grow wild, and in North Korea there are 14 species including 4 species that have not been reported in Korea, including Poa hisauchii Honda and Poa komarovii Roshev. Distributed.

Poa pratensis L. is also called kingweed grass (North Korean grasshopper). It is a perennial herb that commonly grows on riversides, hills, fields and roadsides in Korea. It is widely known and is the most representative Korean-style grass that is most used in parks, gardens, golf courses, stadiums and slopes in temperate and boreal regions.

In Korea, it grows well in barren soil, withstands pests, and has a common resistance to dry and cold-resistance, Zoysia japonica Steud. Field grasses have the disadvantage of low coverage density, dark color, low germination and composition rate, and especially blue color for only about 6 months of the year. Currently, all of the grasses that are used commercially in Korea are foreign imported species, and are not adapted to the climate characteristics of Korea. Therefore, if the careful management is not followed after the creation of the grass, there are various problems such as rapid decline in growth.

Therefore, the present inventors have continuously compared and observed morphological and cultivative aspects of wild bovine follicles and introduced species collected from all over Korea. In addition to excellent grassy properties such as texture and texture, they also have good growth characteristics such as early coverage, seed abundance, disease resistance, and dry resistance, and found that this has the optimal characteristics to replace existing imported species. Comparison and analysis using the Random Amplified Polymorphic DNA analysis (DNA fingerprinting) confirmed the presence of biochemical markers that could distinguish "green" grass from other native and introduced species. It is important to provide information about the varieties of the "blues" Wangpoa grass by distinguishing them from other systems and varieties. Discovered the ability and came to complete the present invention.

Therefore, in one aspect, an object of the present invention is to provide a new breed of "green grass" Wangpoa grass, which has both excellent grass color properties, excellent coverage, early coverage, seed abundance, disease resistance, and dry resistance. To provide.

In a further aspect, an object of the present invention is to select the "blue" Wangpoa grass specific DNA label by comparing the Wangpoa pools and introduced species collected from all over the country using DNA fingerprinting, through which the other systems and It is to provide a foundation that can be distinguished from varieties.

1 is a photograph of the blue field Wangpoa grass of the present invention.

2 is a DNA fingerprint of "green" Wangpoa turf compared with 11 varieties of native collection of wild oysters in Korea, 5 varieties of Wangpoa turf including Midnight and field grass. Lane 0: molecular weight marker, lane 1: Destiny, lane 2: Glade, lane 3: Nassau, lane 4: Cheri, lane 5: 20, lane 6: "bluefield wangpoa grass", lane 7: 56, lane 8: 61, lane 9: 65, lane 10: 77, lane 11: 97, lane 12: 109, lane 13: 123, lane 14: 129, lane 15: 132, lane 16: 140, lane 17: Midnight, lane 18: Grass.

3 is a dendrogram showing the relationship between native and introduced Wangpoa grass. 38-"Purples" Wangpoazandi, C-1: Destiny, C-2: Glade, C-3: Nassau, C-4: Cheri, C-5: Midnight-Introduction Wangpoazandi, 20, 56, 123 , 132, 77, 129, 97, 61, 65, 109, 149-domestic collection Wangpoa grass.

Hereinafter, the present invention will be described in more detail.

In Korea's Sanya, a variety of Wangpoapur grows as native plants, and these are ecological species that are highly adapted to Korea's climatic conditions. They are selected from high quality species through their collection, selection and breeding, and commercially developed. Can be replaced.

Therefore, in one aspect, the present invention provides a new breed of "blues" Wangpoa grass, which has both grassy properties excellent in color, grass length, and texture, and excellent growth characteristics such as early coverage, seed multitude, disease resistance, and dry resistance. do.

The present inventors cultivated more than 110 strains of Wangpoapur collected through the collection activities from 1995 to the present, and has the most excellent characteristics as grass through the morphological, cultivar and genetic characteristics as described in the following examples. Blue wild king Poa grass was selected.

Wangpoapul usually lasts more than 10 months of green color to produce green landscapes throughout the year, and has excellent characteristics such as early germination speed and spreading sideways and high yield of seeds with high yield of seeds. Since it is adapted to Korea's environment, it has excellent advantages such as disease resistance, dryness resistance, and resistance to weathering. Therefore, developing grass that best fits the domestic soil based on these characteristics also has the effect of import substitution.

The blue field Wangpoa grass selected by the present inventors has a longer green period compared to Korean grasses such as field grass or gold grass, enabling the production of blue landscapes in all seasons except cold weather, and early germination rate and seed spreading fast and spread well laterally. It has many characteristics such as disease resistance, dryness resistance, and resistance resistance, because it has excellent characteristics such as high yield of seeds with high yields.

According to a further aspect of the present invention by selecting the DNA markers specific to the "blue" Wangpoa grass by comparing the Wangpoa pools and introduced species collected from all over the country using DNA fingerprinting method, and through the other systems and varieties A distinguishable foundation is provided. In other words, the collected grasses were compared and analyzed using the RAPD (Random Amplified Polymorphic DNA) method (DNA fingerprinting) to identify the presence of biochemical markers that can distinguish the "green" grass from other native and introduced species. In addition, it is possible to provide information on the varieties of "blue" Wangpoa grass by distinguishing them from other lines and varieties by revealing the genetic relationship in the genus Poa.

According to the results of RAPD analysis, native native Wangpoa grass was divided into 15 bands by OPB-07 primer, and "blue" Wangpoa grass was found in G, I, K, M unlike other Wangpoa grass. The band appeared. In addition, RAPD data using 6 primers were synthesized and statistically analyzed, and the result was shown as the dendrogram of FIG. And Destiny have a close relationship with Midnight, Nassau, and Cheri. In addition, the "blue" Wangpoazandi was found to have a somewhat similarity to 129 and 97, the native collection species belonging to group II, and a somewhat far similarity to 38, respectively. In addition, other native species belonged to group I and group III and were distinguished from the "green" Wangfoa grass. Therefore, it is possible to genetically distinguish blue turf wangpoa grass of the present invention from other turf species by using the OPB-07 primer as a RAPD assay marker.

Hereinafter, the present invention is described in more detail by the following representative examples, but the present invention is not limited in any way by these examples.

<Example>

Example 1: Harvesting Grass

The present invention was prepared by planting the 'greens' Wangpoagrass, harvested on May, 1995, at the roadside of Yasan, Naeduk-dong, Cheongju-si, Chungcheongbuk-do, on the Sewon Botanical Research Institute in Nonsan, Chungnam, Korea. Analysis was performed.

Example 2: Morphological Characterization

In order to understand the morphological characteristics of the collected Wangpoa grass, the grass length, leaf length, leaf width, first leaf branching, water extraction length, and ear length were collected from May 5, 1997 to May 6, 1997 at Cheongju University At the time of extraction, the height was measured on May 17, 1999, and the yield on June 7, 1999 at Nonsan Farm of Sewon Botanical Research Institute. The plant height is the maximum height of Wangpoa grass, the leaf length is the length of the second leaf, the leaf width is the maximum leaf width of the second leaf, the first leaf branch is the height of the branch of the first leaf, and the length of the water exit is The length of the stem, excluding the ear of the city, was measured and averaged by the length of the ear when measuring the number of ears 5 times, and the height of the exit was based on the maximum height at the time of exit, and the quantity of the exit was from the minimum 1 to the maximum 5. Were measured respectively. Morphological and physiological characteristics of the collected grasses are shown in Table 1 below.

Morphological Characteristics of Wangpoa Grass Collected and Introduced in Korea Collected and introduced species Extra long (cm) Leaf Length (cm) Leaf width (cm) First leaf branch (cm) Exit diameter length (cm) Isaac length (cm) Extra Length (cm) Water output amount (1-5) 'Blue Field' Wangpoa Grass 28.2 17.3 2.9 6.8 40 7.9 64 4.5 77 21.6 9.6 4.1 5.0 37.8 7.3 33 3.0 Glade 27.1 13.8 2.9 6.1 - - 42 4.0 Cheri 17.1 12.6 2.8 5.7 - - 39 2.0 65 26.3 25.0 2.5 6.7 56.5 8.0 58 4.0 Destiny 27.8 12.7 3.5 9.1 - - 53 2.5 61 25.3 21 2.8 5.4 51.8 9.2 46 3.0 Nassau 19.0 12.5 3.0 6.0 - - 75 2.0 129 14 9.0 4.4 2.9 37.5 8.1 60 2.5 109 19.2 16.1 2.3 4.9 27.9 6.1 49 2.0 56 31.9 26.6 2.7 11.4 48.8 8.9 76 2.0 132 33.2 21.7 2.2 4.3 62.4 10.0 73 2.0 97 20.2 14.0 2.4 4.1 30.7 7.7 43 1.0 20 24.6 11.0 2.4 4.3 41.1 6.8 79 2.0 123 16.8 14.5 2.5 2.5 35.7 7.1 70 2.0 Average 23.48 15.82 2.89 5.68 31.34 5.80 57.33 2.56 LSD (0.05) 2.4 5.2 0.6 1.7 4.1 1.3 - -

Glade, Cheri, Destiny, Nassau

2. 77, 65, 61, 129, 109, 56, 132, 97, 20, 123: domestic collection Wangpoa grass

3. LSD (0.05): statistically significant difference of difference at 95% level

From the results in Table 1, 'Blue' Wangpoa Grassy has a height of 28.2 cm, a length of 17.3 cm, a width of 2.9 cm and a first leaf branch of 6.8 cm. The length of the exit diameter, the ear length of 7.9 cm, and the exit volume of 4.5 are shown. Plant height, leaf length, first leaf branching, plant height, water diameter, length of ear, etc. were slightly larger than the average value of Wangpoa grass, but the leaf width was average and the yield was the highest. It was found to be.

Example 3: Visual Quality Assessment

In order to evaluate the quality of wangpoa grass, visual evaluation was carried out on 11 domestically collected species and 4 introduced species including blue grass wangpoa grass. In other words, the evaluation was performed in 1 to 5 stages in which the lowest quality was 1 point and the highest quality was 5 points. In general, the visual evaluation of turf is generally applied to 1 to 9 levels, but in this study, since the collected Wangpoa grass is grown in the natural state, the quality difference between individuals is not expected to be large. Was applied. The evaluation was conducted once on May 23, 1998, and six times in 1999, five times from spring to autumn. The results are shown in Table 2 below.

Visual Quality Evaluation of Wangpoa Grass Collected and Introduced in Korea Collected and introduced species Date of Evaluation Average 98/5/23 99/4/19 99/6/7 99/8/13 99/9/29 99/10/20 Blue Wild Wangpoa Grass 4.0 3.0 3.5 3.5 4.5 4.5 3.8 77 5.0 5.0 3.5 3.5 4.3 3.8 4.2 Glade 5.0 5.0 4.5 1.5 4.0 4.0 4.0 Cheri 4.0 4.5 2.5 3.0 4.3 3.0 3.6 65 4.0 4.0 3.5 2.0 3.5 3.8 3.5 Destiny 4.0 5.0 3.0 2.0 3.0 3.5 3.4 61 3.5 3.0 2.5 3.0 3.3 3.5 3.1 Nassau 4.5 4.0 1.5 3.5 2.0 3.0 3.1 129 4.0 5.0 3.0 1.0 1.3 2.0 2.7 109 2.0 4.0 2.5 2.0 3.0 2.5 2.7 56 2.0 4.0 2.0 2.0 2.0 2.5 2.4 132 2.5 4.0 2.0 2.0 1.8 2.0 2.4 97 4.0 3.0 2.0 2.0 1.5 1.5 2.3 20 4.0 1.5 2.5 1.5 1.5 2.0 2.2 123 2.5 4.0 2.0 1.0 1.0 1.5 2.0

Glade, Cheri, Destiny, Nassau

2. 77, 65, 61, 129, 109, 56, 132, 97, 20, 123: domestic collection Wangpoa grass

As a result of the evaluation, the visible quality between the collected and introduced species showed a lot of difference, and the 'blues' Wangpoa grass showed an average of 3.8, which showed the excellent quality among the analyzed Wangpoa grasses. In other words, Wangpoagrass, a 'green field', showed excellent visual characteristics following native collection No. 77 and introduced breed glade, indicating that it has excellent advantages such as disease resistance, drying resistance, and resistance to adaptation to Korean environment.

Example 4 Growth Characteristics

In order to understand the growth characteristics of the 'green field' Wangpo Agrass, five kinds of seeds including the 'green field' Wangpo azad showed excellent characteristics among the collected royal poach grass. It was sown in the package on the month 14 After planting, management work such as watering, fertilization and cutting were carried out conventionally.

On May 7, 2000, the characteristics of coverage, visual evaluation, incidence, color, etc., showed that 'blues' Wangpoagrass had high coverage and visual evaluation, low incidence, and bright green color. The results are shown in Table 3. That is, it was judged to have excellent turfgrass characteristics with early coverage and adaptability to Korean environment.

Collection and characteristics of introduced species Wangpoa grass Collected and introduced species Coverage% Visual assessment (1-9) Incidence (%) color Blue Field Wangpoa Grass 90.0 7.0 5.0 Light green 77 73.3 6.8 0.0 Dark green 58 93.3 5.3 25.0 Light green 65 96.7 5.3 32.0 Light green 41 96.7 5.7 28.3 Light green Midnight 70.0 6.7 0.0 Dark green Blacksburg 70.0 6.5 0.0 Dark green Unique 70.0 6.3 0.0 Medium green Award 70.0 6.7 0.0 Dark green LSD (0.05) 13.9 0.4 9.1 -

Midnight, Blacksburg, Unique, Award

2. 77, 58, 65, 41: domestic collection Wangpoa grass

3. LSD (0.05): statistically significant difference of difference at 95% level

Example 5 Classification of Blue Field Wangpoa Grass using RAPD

To identify the taxonomic location of the 'blues' Wangpo Agrass and to confirm the relationship between the Wangpo agrasses, we compared the turf grasses of 12 domestic collections and 5 species of introduced varieties including the 'blues' Wangpo agrass. The plants were subjected to RAPD analysis. As primers used for RAPD, six kinds of operon primers OPB-04, OPB-05, OPB-07, OPB-08, OPB-10, and OPB-18 were used. The results of the RAPD profile are as shown in FIG.

A total of 102 polymorphic bands were obtained, and the band patterns were different between the Wangpoa grass introduced varieties and native collection species in all random primers used in the experiment. Among the six primers, OPB-07 was encoded as shown in Table 4. The band was divided into 15 bands by the OPB-07 primer, and the "blue" Wangpoagrass was found to appear in G, I, K, and M, unlike other Wangpoagrass. In addition, six primer data were synthesized and statistically analyzed and represented as the dendrogram of FIG. 3.

As a result, Wangpoagrass is divided into 3 groups, and 'Blue' Wangpoagrass is belonging to the same group II with the introduced species Glade, Destiny, Midnight, Nassau, and Cheri, and 77 and 97, respectively. Within Group II, these similarities were found to be quite different. In addition, other native species belonged to group I and group III and were found to be quite distinct from the 'blues' Wangpoagrass. Based on the RAPD analysis, it was found that Wangpoagrass, which is 'blue', is clearly distinguished from the introduced and autogenous species.

Data of OPB-07 Primer Lanes One 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 OPB-07 A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 One B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 One C 0 0 0 0 One 0 One One One 0 One One One 0 One One 0 0 D 0 0 0 0 0 0 0 0 0 0 One 0 One 0 0 0 0 0 E One One One One 0 0 0 One One One One One 0 One 0 One 0 0 F 0 0 0 0 0 0 0 0 0 0 One 0 0 0 0 0 0 0 G 0 0 0 0 0 One 0 0 0 0 0 0 0 0 0 0 0 0 H One One 0 0 One 0 One 0 0 One One 0 One 0 0 0 0 0 I One One One One One One One One One One One One One One One One One 0 J 0 0 0 0 0 0 0 One One 0 0 One 0 0 0 One 0 0 K 0 0 0 0 0 One 0 0 0 0 One 0 0 One 0 0 0 0 L 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 One M 0 0 0 0 One One One One 0 0 0 One One One One One 0 0 N 0 0 One One One 0 One One One 0 0 One One 0 One One One 0 0 One One 0 One 0 0 0 0 0 One 0 0 0 One 0 0 0 0

Lane 1: Destiny, Lane 2: Glade, Lane 3: Nassau, Lane 4: Cheri, Lane 5: 20, Lane 6: "Blues" King Poazandi, Lane 7: 56, Lane 8: 61, Lane 9: 65 , Lane 10: 77, lane 11: 97, lane 12: 109, lane 13: 123, lane 14: 129, lane 15: 132, lane 16: 140, lane 17: Midnight, lane 18: field grass [the present invention new breed blue Asexual breeding method of wild wangpoa grass] The asexual breeding method of new varieties of blue wild wangpoa grass of the present invention was carried out as the following experimental example. [Experimental Example 1] [Proliferation Method by Spriging] Sprig, which is a part of the grass plant including the underground diameter of the grass, is cut to include a node and planted 5-7cm deep at 20 ~ 40cm intervals. Provide plenty of moisture after planting. As a result, pedicles and roots emerged from each node to form a lawn. [Experimental Example 2] [Proliferation Method by Sodding] The new varieties of blue wild wangpoa grass of the present invention were wide and 60-180 cm long and 40-45 cm wide. Cut flat square turf sod to a certain size and plant it at regular intervals. As a result, the empty space between the turf was filled by the underground diameter, and the lawn was formed. [Experimental Example 3] [Proliferation Method by Plugging] The plug of a new grass of the present invention blue sage Wangfoa grass is cylindrical or block-shaped small grass pieces ( Split it into plugs and plant it at regular intervals. As a result, the empty space between the plugs was filled by the ground crawling and underground diameters to form a lawn. [Experimental Example 4] [Method of Regenerating Plants from Callus] MS medium containing 1mg / L of 2,4-D (2,4-dichlorophenoxyacetic acid) (KNO3 1900mg / L, NH4NO3 1650mg / L, MgSO4 · 7H2O 370mg / L, KH2PO4 170mg / L, CaCl2 2H2O 440mg / L, H2BO3 6.2mg / L, MnSO4 · 4H2O 22.3mg / L, ZnSO4 · 7H2O 8.6mg / L, NaMoO4 · 2H2O 0.25mg / L, CuSO4 · 5H2O 0.025mg / L, CoCl2 · 6H2O 0.025mg / L , KI 0.83mg / L, FeSO47H2O 27.8mg / L, disodium EDTA 37.3Mg / L, Glysine 2mg / L, sucrose 30 × 103mg / L, Thiamine hydrochloride 0.5mg / L, Pyridoxine hydrochloride 0.5mg / L, Nicotinic acid 0.5 mg / L, myo-inositol 100 mg / L, pH 5.7) induce callus. The induced callus is transferred to a 1/2 concentration of MS medium without hormones to regenerate the plant. The regenerated plant is purified and grown in a greenhouse. [Experiment 5] Wangpoa grass green grass is also capable of seed propagation. In this case, more than 95% of seeds are produced by unit reproduction, which is considered to be asexual propagation. . The individual plants collected from green field seeds and the lawns planted at regular intervals like plugs had the same vegetative characteristics as those formed by herds or plugs.

The blue field Wangpoa grass of the present invention has a longer green period compared to Korean grasses such as field grass or gold grass, enabling the production of blue landscapes in all seasons except cold weather, and the early germination rate and the yield of seeds spread well laterally. It has many characteristics such as disease resistance, dry resistance, and resistance because it has been adapted to the Korean environment for a long time as well as having excellent characteristics such as many seeds, many probability.

Claims (3)

Blue period is about 10 months, leaf color is bright green, multiply broad mesenchymal solid form, average length of grass length is 28.2cm, leaf length is 17.3cm, leaf width is 2.9cm, first leaf branching Is 6.8㎝, grass length is 40cm, water exit diameter is 64cm, ear length is 7.9cm, water output is 4.5, and it has morphological characteristics and it is planting grass plant or grass forge with underground diameter. Or Poa pratensis L., named Bluefield Wangpoagrass, characterized by midwifery. Wangpoa grass according to claim 1, characterized by the characteristic band in G, I, K, M by RAPD analysis using OPB-07 primer (manufactured by Operon). delete