KR100419719B1 - New turfgrass cultivar of Sewon Poa pratensis L. - Google Patents

Abstract

The present invention relates to a new species "Sewon" wangpoa grass ( Poa pratensis L.) of domestic native wangpoa grass (English name: Kentucky bluegrass). The "sewon" Wangpoa grass of the present invention has a longer green period than Korean grasses such as field grass or gold grass, so it is possible to produce a blue landscape in all seasons except cold weather, and the leaf color is dark, and the quality is excellent because it is a dwarf molding. As it is smeared, it has excellent morphological characteristics such as excellent density characteristics and has been adapted to the Korean environment for a long time. Therefore, it has advantages of disease resistance, dry resistance, and resistance against introduced foreign species.

Description

New turfgrass cultivar of Sewon Poa pratensis L.

The present invention relates to a new species "Sewon" wangpoa grass ( Poa pratensis L.) of domestic native wangpoa grass (English name: Kentucky bluegrass). More specifically, the grass collected from the river coriander site in the Gamcheon-ri, Cheongcheon-myeon, Goesan-gun, Chungcheongbuk-do, Korea was classified by DNA fingerprinting method, and the genetic linkage in the genus of "Sewon" Wangpoagrass (Kentucky Bluegrass) was identified and the domestic collection species and introduction It is about identifying taxonomic positions with species and raising them as new varieties of Wangpoagrass.

In Korea, Poa spp . Is a plant of Poa pratensis L., Poa nipponica Koidz., Poa annua L., Poa compressa L., 18 species, including grass ( Poa radula Fr. et Sav.) Grow wild, and in North Korea there are 14 species including 4 species that have not been reported in Korea, including Poa hisauchii Honda and Poa komarovii Roshev. Distributed.

Poa pratensis L. is also called kingweed grass (North Korean grasshopper). It is a perennial herb that commonly grows on riversides, hills, fields and roadsides in Korea. It is widely known and is the most representative Korean-style grass that is most used in parks, gardens, golf courses, stadiums and slopes in temperate and boreal regions.

In Korea, it grows well in barren soil, withstands pests, and has a common resistance to dry and cold-resistance, Zoysia japonica Steud. Field grasses have the disadvantage of low coverage density, dark color, low germination and composition rate, and especially blue color for only about 6 months of the year. Currently, all the grasses that are used commercially in Korea are foreign imported species, which are not adapted to the climate characteristics of Korea. Therefore, if the careful management is not followed after the grass is made, there are various problems such as rapid decline in growth.

Therefore, the present inventors have continuously compared and observed morphological and cultivation aspects of native wangpoapulum and introduced species collected from all over the country, the "sewon" grass compared to other native species and introduced species, color, height, Not only the grassy properties such as texture were excellent but also the growth characteristics such as disease resistance and dry resistance were found to be optimal for replacing existing imported species, and furthermore, they were analyzed by RAPD (Random Amplified Polymorphic DNA) analysis method ( DNA fingerprinting) confirmed the presence of biochemical markers that could distinguish "Sewon" grass from other native and introduced species, as well as revealing the genetic relationship within Poa. Completing the present invention by discovering that it is possible to provide information on varieties of Azandi to distinguish them from other systems and varieties. Reached.

Accordingly, an object of the present invention in one aspect is to provide a new breed "Sewon" Wangpoa grass having both a grassy property excellent in color, grass length, texture, etc., and growth characteristics excellent in disease resistance, dry resistance and the like.

In a further aspect, an object of the present invention is to select the "sewon" Wangpoa grass specific DNA markers by comparing the Wangpoa pools and introduced species collected from all over the country using DNA fingerprinting, through which the different lines and varieties To provide a foundation that can be distinguished from

Figure 1 is a photograph of the discharge of Sewon Wangpoa grass of the present invention.

FIG. 2 shows the DNA fingerprints of "Sewon" Wangpoagrass compared to 11 varieties of native Korean varieties of Wangpoagrass, 5 introduced varieties of Wangpoagrass, including Midnight. Lane 0: Molecular Weight Marker, Lane 1: Destiny, Lane 2: Glade, Lane 3: Nassau, Lane 4: Cheri, Lane 5: 20, Lane 6: 38, Lane 7: 56, Lane 8: 61, Lane 9: 65 Lane 10: Sewon Wangpoa Grass, Lane 11: 97, Lane 12: 109, Lane 13: 123, Lane 14: 129, Lane 15: 132, Lane 16: 140, Lane 17: Midnight, Lane 18: Grass.

3 is a dendrogram showing the relationship between native and introduced Wangpoa grass. 77-"Sewon" Wangpoagrass, C-1: Destiny, C-2: Glade, C-3: Nassau, C-4: Cheri, C-5: Midnight-Introduced Wangpoagrass, 20, 56, 123, 132, 38, 129, 97, 61, 65, 109, 149

Hereinafter, the present invention will be described in more detail.

In Korea's Sanya, a variety of Wangpoapur grows as native plants, and these are ecological species that are highly adapted to Korea's climatic conditions. They are selected from high quality species through their collection, selection and breeding, and commercially developed. Can be replaced.

Accordingly, in one aspect, the present invention provides a new breed "Sewon" Wangpoa grass, which has both grassy properties excellent in color, grass length, and texture, and growth characteristics excellent in disease resistance and dry resistance.

The present inventors cultivated more than 110 strains of Wangpoapur collected through the collection activities from 1995 to the present, and has the most excellent characteristics as grass through the morphological, cultivar and genetic characteristics as described in the following examples. Sewon Wangpoagrass was selected.

Wangpoa grass usually lasts for more than 10 months and can produce green landscapes throughout the year. Its rapid rate of formation makes it useful for early greening, and it is well-grown in shade or wetlands. Developing the best grass for domestic soil will also have the effect of import substitution.

Sewon Wangpoa grass selected by the present inventors has a longer green period than Korean grasses such as field grass or gold grass, enabling the production of blue landscapes in all seasons except cold weather, and the leaf color is dark, and the shape is excellent, so the quality is excellent and sideways. Since it spreads well, it has excellent morphological characteristics such as excellent density characteristics and has been adapted to the Korean environment for a long time, and thus has excellent advantages such as disease resistance, dry resistance, and resistance against introduced species.

According to a further aspect of the present invention, by comparing the DNA and Wangpoa pools and introduced species collected from all over the country using the DNA fingerprinting method "SEWON" Wangpoa grass specific DNA markers to be selected, through which can be distinguished from other lines and varieties A foundation is provided. In other words, the collected grasses were compared and analyzed using the RAPD (Random Amplified Polymorphic DNA) method (DNA fingerprinting) to identify the presence of biochemical markers that can distinguish "SEWON" grass from other native and introduced species. In addition, by revealing the genetic relationship in the genus Poa, it is possible to provide information on the varieties of "Sewon" Wangpoa grass, to distinguish them from other systems and varieties.

According to the results of RAPD analysis, native native wangpoa grass was divided into 15 bands by OPB-07 primer, and "sewon" wangpoa grass was C, D, E, F, H unlike other wangpoa grass. It was found that the band appeared at, I, K. In addition, RAPD data using 6 primers were synthesized and statistically analyzed, and the result was shown as the dendrogram of FIG. 3. Wangpoa grass was divided into 3 groups, and "Sewon" Wangpoa grass was in group Ⅱ and introduced species Glade and It has a close relationship with Destiny, but with a somewhat distant relationship with Midnight, Nassau and Cheri. In addition, the "Sewon" Wangpo Azandi has a similarity to the native collection species belonging to group II, 129 and 97, and similarity to distant from 38, respectively. In addition, other native species belonged to group I and group III and were found to be significantly different from the "sewon" Wangpoa grass. Therefore, it is possible to genetically distinguish Sewon wangpoa grass of the present invention from other turf species using the OPB-07 primer as a RAPD assay marker.

Hereinafter, the present invention is described in more detail by the following representative examples, but the present invention is not limited in any way by these examples.

<Example>

Example 1: Harvesting Grass

The present inventors planted Sewon Wangpoa grass collected on May 5, 1995 at Gwamyeong-ri, Cheongcheon-myeon, Goesan-gun, Gocheong-gun, Chungcheongbuk-do, at the Sewon Botanical Research Institute in Nonsan, Chungnam, Korea. Analysis was performed.

Example 2: Morphological Characterization

In order to understand the morphological characteristics of the collected Wangpoa grass, the grass length, leaf length, leaf width, first leaf branching, water extraction length, and ear length were collected from May 5, 1997 to May 6, 1997 at Cheongju University The harvest height was measured on May 17, 1999 and the yield was on June 7, 1999 at Nonsan Farm of Sewon Plant Research Institute. The plant height is the maximum height of Wangpoa grass, the leaf length is the length of the second leaf, the leaf width is the maximum leaf width of the second leaf, the first leaf branch is the height of the branch of the first leaf, and the length of the water exit is The length of the stem excluding the ear of the stem and the ear length are the average values of the length of the ear when measuring the number of times 5 times, respectively. It was measured based on each. Morphological and physiological characteristics of the collected grasses are shown in Table 1 below.

Morphological Characteristics of Wangpoa Grass Collected and Introduced in Korea Collected and introduced species Extra long (cm) Leaf Length (cm) Leaf width (cm) First leaf branch (cm) Exit diameter length (cm) Isaac length (cm) Extra Length (cm) Water output amount (1-5) Sewon Wangpo Agrass 21.6 9.6 4.1 5.0 37.8 7.3 33 3.0 Glade 27.1 13.8 2.9 6.1 - - 42 4.0 38 28.2 17.3 2.9 6.8 40 7.9 64 4.5 Cheri 17.1 12.6 2.8 5.7 - - 39 2.0 65 26.3 25.0 2.5 6.7 56.5 8.0 58 4.0 Destiny 27.8 12.7 3.5 9.1 - - 53 2.5 61 25.3 21 2.8 5.4 51.8 9.2 46 3.0 Nassau 19.0 12.5 3.0 6.0 - - 75 2.0 129 14 9.0 4.4 2.9 37.5 8.1 60 2.5 109 19.2 16.1 2.3 4.9 27.9 6.1 49 2.0 56 31.9 26.6 2.7 11.4 48.8 8.9 76 2.0 132 33.2 21.7 2.2 4.3 62.4 10.0 73 2.0 97 20.2 14.0 2.4 4.1 30.7 7.7 43 1.0 20 24.6 11.0 2.4 4.3 41.1 6.8 79 2.0 123 16.8 14.5 2.5 2.5 35.7 7.1 70 2.0 Average 23.5 15.8 2.7 5.7 45.6 8.4 57.3 2.6 LSD (0.05) 2.4 5.2 0.6 1.7 4.1 1.3 - -

Glade, Cheri, Destiny, Nassau

2. 38, 65, 61, 129, 109, 56, 132, 97, 20, 123: domestic collection Wangpoa grass

3. LSD (0.05): statistically significant difference of difference at 95% level

From the results in Table 1, "Sewon" Wangpoa grass has a height of 21.6 cm, a length of 9.6 cm, a width of 4.1 cm, and a first leaf branch of 5.0 cm. A hydraulic diameter length, the ear length of 7.3 cm, and the water output amount of 3 are shown. At the time of harvesting, the grass length was the smallest among the surveyed Wangpoa grasses. Grass was found to be broadleaf dwarf

Example 3: Visual Quality Assessment

In order to evaluate the quality of Wangpo Azuri, visual evaluation was applied to 11 domestic collection species and 4 introduced species, including Sewon Wangpoagrass. In other words, the evaluation was performed in 1 to 5 stages in which the lowest quality was 1 point and the highest quality was 5 points. In general, the visual evaluation of turf is generally applied in 1 to 9 stages, but in this study, since the collected Wangpoa grass was grown in the natural state, the quality difference between individuals is not expected to be large. Was applied. The evaluation was conducted once on May 23, 1998, and six times in 1999, five times from spring to autumn. The results are shown in Table 2 below.

Visual Quality Evaluation of Wangpoa Grass Collected and Introduced in Korea Collected and introduced species Date of Evaluation Average 98/5/23 99/4/19 99/6/7 99/8/13 99/9/29 99/10/20 Sewon Wangpo Agrass 5.0 5.0 3.5 3.5 4.3 3.8 4.2 Glade 5.0 5.0 4.5 1.5 4.0 4.0 4.0 38 4.0 3.0 3.5 3.5 4.5 4.5 3.8 Cheri 4.0 4.5 2.5 3.0 4.3 3.0 3.6 65 4.0 4.0 3.5 2.0 3.5 3.8 3.46 Destiny 4.0 5.0 3.0 2.0 3.0 3.5 3.41 61 3.5 3.0 2.5 3.0 3.3 3.5 3.1 Nassau 4.5 4.0 1.5 3.5 2.0 3.0 3.1 129 4.0 5.0 3.0 1.0 1.3 2.0 2.71 109 2.0 4.0 2.5 2.0 3.0 2.5 2.66 56 2.0 4.0 2.0 2.0 2.0 2.5 2.41 132 2.5 4.0 2.0 2.0 1.8 2.0 2.4 97 4.0 3.0 2.0 2.0 1.5 1.5 2.3 20 4.0 1.5 2.5 1.5 1.5 2.0 2.16 123 2.5 4.0 2.0 1.0 1.0 1.5 2.0

Glade, Cheri, Destiny, Nassau

2. 38, 65, 61, 129, 109, 56, 132, 97, 20, 123: domestic collection Wangpoa grass

As a result of the evaluation, the visible quality between collected and introduced species showed a lot of difference, and the "Sewon" Wangpoa grass showed the highest average of 4.2, which showed the best quality among the Wangpoa grasses analyzed. In other words, "sewon" Wangpoagrass has been proven to have excellent advantages such as disease resistance, drying resistance, and resistance against the introduced species because it has been adapted to the Korean environment for a long time as well as other native collection species.

Example 4 Growth Characteristics

In order to understand the growth characteristics of "Sewon" Wangpo Agrass, five kinds of seeds, including "Sewon" Wangpo Agrass, which showed the excellent characteristics among the collected Wangpo azads, were harvested and four kinds of excellent Pokpograss were introduced. Seeds were sown in one package. After planting, management work such as watering, fertilization and cutting were carried out conventionally.

On May 7, 2000, the characteristics of coverage, visual evaluation, incidence, and color showed that "Sewon" Wangpo Azandi had high visual evaluation, no disease, and dark green color. Coverage was lower than that of other collections, but showed similar levels compared to imported species. In other words, it is judged that the grass quality is excellent even in dry and high temperature conditions, and it has excellent grass characteristics that are highly adaptable to the environment of Korea.

Collection and characteristics of introduced species Wangpoa grass Collected and introduced species Coverage% Visual assessment (1-9) Incidence (%) color "Sewon" Wangpoa Grass 73.3 6.8 0.0 Dark green 38 90.0 7.0 5.0 Light green 58 93.3 5.3 25.0 Light green 65 96.7 5.3 32.0 Light green 41 96.7 5.7 28.3 Light green Midnight 70.0 6.7 0.0 Dark green Blacksburg 70.0 6.5 0.0 Dark green Unique 70.0 6.3 0.0 Medium green Award 70.0 6.7 0.0 Dark green LSD (0.05) 13.9 0.4 9.1 -

Midnight, Blacksburg, Unique, Award

2. 38, 58, 65, 41: domestic collection Wangpoa grass

3. LSD (0.05): statistically significant difference of difference at 95% level

Example 5 Classification of Sewon Wangpoa Grass using RAPD

In order to clarify the taxonomic location of Wangpoagrass in Sewon and to confirm the relationship between Wangpoagrass, RAPD analysis was carried out using twelve Korean collections and 5 varieties of Wangpoagrass as a control plant. Was carried out. As primers used for RAPD, six kinds of operon primers OPB-04, OPB-05, OPB-07, OPB-08, OPB-10, and OPB-18 were used. The results of the RAPD profile are as shown in FIG.

A total of 102 polymorphic bands were obtained by this analysis, and the band patterns were different between the Wangpoa grass introduced varieties and native collection species in all random primers used in the experiment. Among the six primers, OPB-07 was encoded as shown in Table 4. Wangpoagrass was divided into 15 bands by the OPB-07 primer, and "Sewon" Wangpoagrass was found in C, D, E, F, H, I, K unlike other Wangpoagrass. there was. In addition, six primer data were synthesized and statistically analyzed and represented as the dendrogram of FIG. 3. As a result, Wangpoagrass was divided into three groups, and "Sewon" Wangpoagrass belongs to group Ⅱ and has close relationship with the introduced species Glade and Destiny, but it is somewhat far from Midnight, Nassau and Cheri. In addition, "Se-won" Wangpoagrass has similarities to 129 and 97, which are indigenous collections of group II, and similar to distant from 38, respectively. In addition, other native species belonged to group I and group III and were found to be significantly different from the "sewon" Wangpoa grass. Based on the RAPD analysis, it was confirmed that "Sewon" Wangpoa grass was genetically distinct from the introduction and autogenous species.

Data of OPB-07 Primer Lanes One 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 OPB-07 A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 One B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 One C 0 0 0 0 One 0 One One One 0 One One One 0 One One 0 0 D 0 0 0 0 0 0 0 0 0 0 One 0 One 0 0 0 0 0 E One One One One 0 0 0 One One One One One 0 One 0 One 0 0 F 0 0 0 0 0 0 0 0 0 0 One 0 0 0 0 0 0 0 G 0 0 0 0 0 One 0 0 0 0 0 0 0 0 0 0 0 0 H One One 0 0 One 0 One 0 0 One One 0 One 0 0 0 0 0 I One One One One One One One One One One One One One One One One One 0 J 0 0 0 0 0 0 0 One One 0 0 One 0 0 0 One 0 0 K 0 0 0 0 0 One 0 0 0 0 One 0 0 One 0 0 0 0 L 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 One M 0 0 0 0 One One One One 0 0 0 One One One One One 0 0 N 0 0 One One One 0 One One One 0 0 One One 0 One One One 0 0 One One 0 One 0 0 0 0 0 One 0 0 0 One 0 0 0 0

Lane 1: Destiny, Lane 2: Glade, Lane 3: Nassau, Lane 4: Cheri, Lane 5: 20, Lane 6: 38, Lane 7: 56, Lane 8: 61, Lane 9: 65, Lane 10: "Sewon Wangpoa grass, lane 11: 97, lane 12: 109, lane 13: 123, lane 14: 129, lane 15: 132, lane 16: 140, lane 17: Midnight, lane 18: field grass Asexual breeding method of Wangpoa grass] The abundant breeding method of the new varieties of green grass Wangpoa grass of the present invention was carried out as follows: Experimental Example 1 [Proliferation Method by Spriging] Sprig, which is a part of the grass plant including the underground diameter of the tree, is cut to include the node and planted 5-7cm deep in 20 ~ 40cm intervals. Provide plenty of moisture after planting. As a result, pedicles and roots emerged from each node to form a lawn. [Experimental Example 2] [Proliferation Method by Sodding] The new varieties of blue wild wangpoa grass of the present invention were wide and 60-180 cm long and 40-45 cm wide. Cut flat square turf sod to a certain size and plant it at regular intervals. As a result, the empty space between the turf was filled by the underground diameter, and the lawn was formed. [Experimental Example 3] [Proliferation Method by Plugging] The plug of a new grass of the present invention blue sage Wangfoa grass is cylindrical or block-shaped small grass pieces ( Split it into plugs and plant it at regular intervals. As a result, the empty space between the plugs was filled by the ground crawling and underground diameters to form a lawn. [Experimental Example 4] [Method of Regenerating Plants from Callus] MS medium containing 1mg / L of 2,4-D (2,4-dichlorophenoxyacetic acid) (KNO3 1900mg / L, NH4NO3 1650mg / L, MgSO4 7H2O 370mg / L, KH2PO4 170mg / L, CaCl2 2H2O 440mg) / L, H2BO3 6.2mg / L, MnSO4 4H2O 22.3mg / L, ZnSO4 7H2O 8.6mg / L, NaMoO4 2H2O 0.25mg / L, CuSO4 5H2O 0.025mg / L, CoCl2 6H2O 0.025mg / L, KI 0.83mg / L, FeSO4 7H2O 27.8mg / L, disodium EDTA 37.3Mg / L, Glysine 2mg / L, sucrose 30 103mg / L, Thiamine hydrochloride 0.5mg / L, Pyridoxine hydrochloride 0.5mg / L, Nicotinic acid 0.5mg / L, myo-inositol 100mg / L, pH 5.7) to induce callus. The induced callus is transferred to a 1/2 concentration of MS medium without hormones to regenerate the plant. The regenerated plant is purified and grown in a greenhouse. [Experiment 5] Wangpoa grass green grass is also capable of seed propagation. In this case, more than 95% of seeds are produced by unit reproduction, which is considered to be asexual propagation. . The individual plants collected from green field seeds and the lawns planted at regular intervals like plugs had the same vegetative characteristics as those formed by herds or plugs.

Sewon Wangpoa grass of the present invention has a longer green period compared to Korean grasses such as field grass or gold grass to enable the directing of the blue scenery in all seasons except cold weather, and the leaf color is dark, dwarf molding, excellent quality, and spread well to the side. It has excellent morphological characteristics such as excellent density characteristics, and has been adapted to the Korean environment for a long time, and thus has excellent advantages such as disease resistance, dry resistance, and resistance against introduced species.

Claims (3)

Blue period is about 10 months, leaf color is dark green, leaf shape is broadleaf dwarf shape, average length of grass length is 21.6cm, leaf length is 9.6cm, leaf width is 4.1cm, Leaf branch is 5.0cm, height is 33cm, water length is 37.8cm, ear length is 7.3cm, water output is morphological characteristics of 3, grass plant or grass forge with underground diameter Poa pratensis L., named Sewon Wangpoagrass, which is characterized by planting or midwifery. Wangpoa grass according to claim 1, characterized by C, D, E, F, H, I, K, characterized by RAPD analysis using OPB-07 primer (manufactured by Operon). delete