KR100682391B1 - Culture medium for growing mushroom contaning by-product produced during a processing of phellinus linteus, method for producung the same, mushroom grown by using the same, and product containing the mushroom or a processed material of the mushroom - Google Patents

Abstract

The present invention relates to a mushroom cultivation medium containing a situation mushroom processing by-product, a method for producing the same, a mushroom grown using the same and a product containing the mushroom or a processed product of the mushroom, and more particularly, Mushroom cultivation medium capable of cultivating mushrooms further comprising the active ingredients of the situation mushrooms remaining in the situation mushroom processing by-products, by using the by-products such as hot water extraction or alcohol extraction of the situation mushrooms, a method of manufacturing the same It relates to a mushroom grown using and to a product containing the mushroom or a processed product of the mushroom.

Mushrooms, Processing, By-Products

Description

CULTURE MEDIUM FOR GROWING MUSHROOM CONTANING BY-PRODUCT PRODUCED DURING A PROCESSING OF PHELLINUS LINTEUS, METHOD FOR PRODUCUNG THE SAME, MUSHROOM GROWN BY USING THE SAME, AND PRODUCT CONTAINING THE MUSHROOM OR A PROCESSED MATERIAL OF THE MUSHROOM}

The present invention relates to a mushroom cultivation medium containing a situation mushroom processing by-product, a method for producing the same, a mushroom grown using the same and a product containing the mushroom or a processed product of the mushroom, and more particularly, Mushroom cultivation medium capable of cultivating mushrooms further comprising the active ingredients of the situation mushrooms remaining in the situation mushroom processing by-products, by using the by-products such as hot water extraction or alcohol extraction of the situation mushrooms, a method of manufacturing the same It relates to a mushroom grown using and to a product containing the mushroom or a processed product of the mushroom.

Phellinus linteus , a perennial mushroom classified as linteus of the Hymenochaetaceae , commonly referred to as situation mushroom, is known to live on mulberry trees. However, the situation mushroom is parasitic to other broad-leaved trees such as cottonwood, alder, poplar, chestnut, etc. in addition to mulberry trees. Medicinal mushrooms have been used for function activation, detoxification and arthritis. In recent years, while the situation mushroom has been reported to have an excellent anti-cancer effect and various physiological activities, it is attracting attention as a material of food as well as medicine.

However, in spite of various excellent physiological activities, since the price of the situation mushroom itself is very expensive, there is a problem that the general public is not easily accessible. In addition, in the case of processing the situation mushroom to extract only the active ingredient, it is common to use a method such as hot water extraction or alcohol extraction, in order to increase the extraction efficiency, if the extraction temperature or other extraction conditions are taken harshly, Since the substance itself is damaged and loses its activity, there are limitations on the extraction conditions that can be actually adopted. Therefore, by-products after hydrothermal or ethanol extraction of situation mushrooms contain relatively significant amounts of situation mushroom active ingredients. Considering its own price, it is required to develop a technology that further utilizes the by-products, but until now all of these by-products have been disposed of.

In addition, unlike the situation mushrooms, which are very difficult to grow, general mushrooms such as oyster mushrooms are relatively easy to grow, and related technologies are also very advanced. Therefore, other common mushrooms containing active ingredients of situation mushrooms are produced at low cost. If so, many people can easily access the useful ingredients of situational mushrooms at low prices, and the development of such related technologies is also called for.

The present invention is to solve the problems of the prior art as described above, an object of the present invention, by utilizing the by-products such as hot water extraction or alcohol extraction of the situation mushroom, which has been discarded until now, the situation mushroom Providing a mushroom cultivation medium capable of cultivating a mushroom further comprising the active ingredients of the situation mushroom remaining in the processing by-product, a method for producing the same, and a mushroom grown using the same and a product containing the mushroom or a processed product of the mushroom will be.

According to the present invention, there is provided a mushroom cultivation medium comprising a by-product mushroom processing by-product.

In the culture medium for mushroom cultivation according to the present invention, the by-product mushroom processing by-product means the residual by-product after obtaining the active ingredient by primarily processing the mushroom, the side to utilize the active mushroom mushroom remaining in the by-product In the above, the by-product mushroom processing by-product is preferably a by-product after processing such as hot water extraction or alcohol extraction of the situation mushroom.

In addition, the by-product mushroom processing by-products should be dried immediately after processing such as hot water extraction or alcohol extraction of the situation mushroom, in order to prevent the occurrence of microbial contamination due to the presence of moisture. In addition, the by-product mushroom processing by-products can be used by grinding after drying, the size of the particles to be pulverized, there is no particular limitation, in consideration of the mixing with other materials, such as sawdust mixed together during the production of the medium From the viewpoint, it is preferable to grind to have an average particle size of 0.1 ~ 5mm.

The mushroom cultivation medium according to the present invention may be made of only the by-product mushroom processing by-products, and may further comprise a material commonly used in a medium for mushroom cultivation, such as sawdust and rice bran, in addition to the situation mushroom processing by-products. When the situation mushroom processing by-products are used together with sawdust, rice bran, etc., it is preferable to include 40 to 400 g of situation mushroom processing by-products per 1000 cc of the mushroom cultivation medium. When the amount of the situation mushroom processing by-products contained in the mushroom culture medium 1000cc is less than 40 g, the amount of the situation mushroom active ingredient in the mushroom culture medium becomes insufficient, and when it exceeds 400 g, it is incompatible with other materials such as sawdust. .

According to another aspect of the invention, the drying step of drying the hot water extraction or alcohol extraction processing by-products of the situation mushroom, the grinding step of grinding the dried by-products to an average particle size of 0.1 ~ 5mm, and the crushed by-products sawdust and Provided is a method for producing a culture medium for mushroom cultivation, comprising a mixing step of mixing.

In the method for producing a culture medium for mushroom cultivation according to the present invention, the drying step is a step of removing moisture in the by-products generated after the hot water extraction or alcohol extraction processing of the situation mushroom, under the conditions of temperature and pressure for water removal It may be carried out using a dryer, but if the temperature is too high, there may be damage to the active ingredient remaining in the by-product, it is preferable that the temperature during drying does not exceed 110 ℃ as possible.

In the method for producing a culture medium for mushroom cultivation according to the present invention, the grinding step is a step of grinding the dried by-products to an average particle size of 0.1 ~ 5mm, it can be performed using a conventional mill. If the average particle size is less than 0.1mm, the particle size is too small, the workability worsens, if the average particle size is more than 5mm, there is a problem that the mixing with the sawdust falls.

In the method for producing a culture medium for mushroom cultivation according to the present invention, the mixing step is a step of mixing the crushed by-products with sawdust, can be performed manually or using a conventional mixer. In the mixing step, the mixing ratio of the dried and pulverized mushroom by-product: sawdust is preferably 0.1 to 1: 1 in weight ratio. When this mixing ratio is less than 0.1: 1, the amount of the situation mushroom active ingredient in the mushroom cultivation medium becomes insufficient, and when it exceeds 1: 1, the mixing efficiency with sawdust is inferior.

In the method for producing a mushroom cultivation medium according to the present invention, in the mixing step, if necessary, additional materials such as rice bran may be further added and mixed.

The mushroom cultivation medium manufacturing method according to the present invention, after the mixing step, may further comprise the step of adjusting the water content of the mixed materials. There is no particular limitation on the moisture content, in terms of general mushroom growth efficiency, it can be appropriately adjusted according to the type of mushroom to be cultivated within the range of 30 to 80%.

According to another aspect of the invention, there is provided a mushroom, which is grown in a mushroom cultivation medium according to the invention.

The mushrooms cultivated in the mushroom cultivation medium according to the present invention will include at least a part of the situation mushroom active ingredient that was present in the mushroom cultivation medium, and thus, more beneficial bioactivity than other mushrooms of the same kind grown in other medium. Has There is no particular limitation on the type of the mushroom according to the present invention, and in terms of antioxidant activity enhancement, it is preferable that the mushroom is oyster mushroom.

According to another aspect of the present invention, there is provided a product containing a mushroom or a processed product of the mushroom grown in the mushroom cultivation medium according to the present invention.

The mushroom or a product containing the processed product of the mushroom according to the present invention, the mushroom grown in the mushroom cultivation medium according to the present invention as it is, or the processed product obtained by processing such as powdered, hot water extraction or alcohol extraction is Included. The mushroom or the product containing the processed product of the mushroom according to the present invention can be used as a functional raw material for the final food product, cosmetics, etc. for the use of food material, cosmetic material and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples, but the scope of the present invention is not limited by these Examples.

EXAMPLE

1. Preparation of mushroom cultivation medium

After extracting the hot water mushrooms, the by-products were dried in a dryer at 60 ° C. for 10 hours to remove moisture. The dried by-products were ground to an average particle size of about 2 mm using a mill. The crushed by-products are mixed with sawdust using a mixer so that the mixing ratio with the sawdust in a weight ratio of by-products: sawdust is 0.3: 1, and there is added fresh rice bran without rice, and rice bran: 0.2: 1 sawdust. Into the mixture was mixed. Next, water was added to the mixture obtained above, adjusted to a water content of 65%, and then blended in a blender to prepare a culture medium for mushroom cultivation.

2. Cultivation of Pleurotus Mushrooms

1000cc of the medium prepared above was put into a PP container for cultivating mushrooms, which was autoclaved for about 5 hours in an autoclave at 121 ° C. After autoclaving, the medium was left at room temperature and cooled to completely remove the heat in the medium, and then inoculated with the Pleurotus spp. And incubated at 25 ° C. for 17 days to grow mycelia. After the medium was sufficiently grown in mycelial growth, the medium was transferred to the cultivation ridge and allowed to grow while maintaining humidity of 90% or more in the cultivation ridge, followed by cultivation for 7 days, to harvest the grown oyster mushroom.

3. Preparation of Pleurotus eryngii Extract Powder

100 g of the harvested oyster mushroom was dried and finely chopped into 3 L of an extraction container, and 1000 ml of 95% ethanol was added thereto, followed by reflux extraction at 80 ° C. for 4 hours. After the extraction was completed, the extract was cooled to room temperature and filtered to separate the extract. The separated extract was concentrated under reduced pressure using a rotary vacuum evaporator to remove the solvent, and then the concentrate was dried with a vacuum pump to obtain 3.8 g of Oyster mushroom extract powder.

The antimicrobial activity of the mushroom extract powder obtained as described above was measured by the following method, and as a result, the relative antioxidant activity of 100% of the ascorbic acid was determined to be 30%.

Antioxidant Activity Measurement Method

As antioxidant activity, lipid peroxidation inhibitory activity was measured by the following method.

Into a 48-well plate, 500 μl of brain homogenate (5 mg protein / ml), 10 μl of sample material, 80 μl of buffer, shake in a 37 ° C. water bath and pre-incubate for 20 minutes. After pre-incubation, 10 μl of 100 μM FeCl ₂ was added thereto, and then incubated at 37 ° C. for 30 minutes. Then, 100 μl of 35% HClO ₄ was added to stop the reaction, and the plate was centrifuged at 2000 rpm for 10 minutes at 4 ° C. After separation, only the supernatant was taken and placed in 400 μl each in an eppendorf-tube, and 200 μl of TBA (5 mg / ml TBA in 50% acetic acid) was added. The tube was reacted at 80 ° C. for 1 hour, cooled to room temperature, and absorbance was measured at 520 nm.

In addition, a calibration curve (standard curve) was obtained by using tetraethoxypropane during TBA reaction, and the amount of MDA generated after the reaction was calculated.

* Antioxidant activity (%) = [(A-B) / A] × 100

(A: nanomolar number / mg protein of MDA (ascorbic acid), B: nanomolar number / mg protein of MDA (sample material)).

4. Preparation of Oyster Mushroom Extract-Containing Products

(1) Preparation of seasonings for cooking

20% by weight of the oyster mushroom extract powder obtained in the same manner as 3, roasted sun salt 60% by weight, roasted sesame seeds 20% by weight to prepare a cooking seasoning.

(2) manufacturing flour food

Bread, pizza, cake, cookies, crackers and noodles were prepared using a flour mixture containing 6 to 10% by weight of the oyster mushroom extract powder obtained as in the above 3.

(3) production of hamburger patties

Hamburger patty containing 6 to 10% by weight of the oyster mushroom extract powder obtained as in the above 3 was prepared.

[Comparative Example]

1. Preparation of mushroom cultivation medium

Fresh rice bran without sawdust and blemishes was mixed so that the rice bran: sawdust was 0.2: 1, water was added to the mixture obtained above to adjust the water content to 65%, and then blended in a blender to prepare a culture medium for mushroom cultivation. .

2. Cultivation of Pleurotus Mushrooms

Using the medium prepared above, oyster mushroom was grown in the same manner as in Example, and harvested.

3. Preparation of Pleurotus eryngii Extract Powder

From 100 g of the Oyster mushrooms harvested above, 3.4 g of Oyster mushroom extract powder was obtained by the same method as in Example.

Antioxidant activity of the mushroom extract powder obtained as described above was measured in the same manner as in Example, and as a result, the relative antioxidant activity of the ascorbic acid at 100% was determined to be 21%.

As can be seen from the antioxidant activity measurement results, the oyster mushroom grown using the mushroom cultivation medium according to the present invention exhibited about 50% improved antioxidant activity compared to the oyster mushroom cultivated using the normal sawdust medium.

In addition, as a result of performing the sensory evaluation on the cooking seasoning, flour food and hamburger patty prepared using the extract powder of oyster mushroom cultivated using the mushroom cultivation medium according to the present invention, the flavor of the oyster mushroom as it is appear.

As described above, according to the present invention, it is possible to cultivate mushrooms which can include active ingredients of physiological mushrooms exhibiting physiological activities such as antioxidant activity, inexpensively and easily, using foodstuffs of the mushrooms or processed products of the mushrooms. In the case of manufacturing such products, it is possible to obtain a product containing the active ingredients of the situation mushroom while maintaining the flavor of the mushroom itself.

Claims (13)

A medium for cultivating oyster mushrooms, including a by-product mushroom processing byproduct The culture medium for growing oyster mushrooms according to claim 1, wherein the by-product mushroom processing by-products are by-products after hot water extraction or spirit extraction of the situation mushrooms. [Claim 3] The medium for growing oyster mushrooms according to claim 2, wherein the by-product mushroom processing by-products are dried immediately after hot water extraction or spirit extraction of the situation mushrooms. According to claim 1, wherein the by-product mushroom processing medium for growing oyster mushroom, characterized in that it is ground to have an average particle size of 0.1 ~ 5mm. The medium for cultivating oyster mushrooms according to claim 1, further comprising sawdust, rice bran, or sawdust and rice bran. The cultivation medium for oyster mushroom according to claim 1, comprising 40 to 400 g of the situation mushroom processing by-product per 1000cc of the cultivation medium for oyster mushroom. It comprises a drying step of drying the hot water extraction or alcohol extraction processing by-products of the situation mushroom, a grinding step of grinding the dried by-products to an average particle size of 0.1 ~ 5mm, and a mixing step of mixing the crushed by-products with sawdust , Pleurotus cultivation medium production method. The method of claim 7, wherein the mixing ratio in the mixing step is pulverized by-product mushrooms: sawdust is 0.1 to 1: 1 by weight ratio production method for cultivating oyster mushroom. 8. The method of claim 7, further comprising adjusting the water content of the mixed materials after the mixing step. The oyster mushroom, characterized in that it was cultivated in the medium for growing oyster mushroom according to any one of claims 1 to 6. delete Food product characterized by containing the processed material of the oyster mushroom or its mushroom cultivated in the medium for growing oyster mushroom according to any one of claims 1 to 6. The food product according to claim 12, wherein the processed material of the oyster mushroom is obtained by pulverizing the oyster mushroom, hot water extraction or alcohol extraction.