KR100663094B1 - Bioluminescence measuring apparatus and the vial holder - Google Patents

Abstract

Provided are a device for automatically measuring toxicity in water by using bioluminescence bacteria by which toxicity degree can be accurately measured for predetermined period periodically and continuously by unmanned driving mode, and a reactor vessel for the bacteria used in the device. The device comprises a transferring part for vessel, a light receiving sensor part(8), a power part for driving the transferring part and the sensor(8), a controller connected to the sensor part(8), and a part for automatically injecting bioluminescence bacteria and raw water for water analysis. The transferring part comprises a plurality of vessels(2) connected by a pair of rings so as to form a chain; a plurality of sprockets for transferring the plurality of vessels(2); a plurality of roller bearings for facilitating the sprockets to smoothly rotate; and a driving motor for driving the sprockets. The plurality of vessels(2) have vials in their cylinders respectively. The sensor part(8) comprises a light receiving sensor(14) for detecting an amount of light emitted from the bioluminescence; a vertically reciprocating member(18) for vertically blocking the vessels(2); a horizontally reciprocating member(19) for horizontally pushing the vessels(2) for side-blocking; a plurality of bars(16) connected to the members(18,19) by hinge; a roller bearing(23) contacting with a cam(24) so as to transmit a displacement of the cam to the bars(16); two cams for laterally moving the bars(16); a gear line(22) for driving the cams(24); a driving motor(46) for driving the gear line; a push stick for reactor vessel which pushes the vessels(2) at predetermined distance so as to separate the vessels(42) from the side block; and a compression spring(43) which applies a force for pushing the push stick(42) in direction of the vessel(2).

Description

Automatic device for measuring water toxicity using luminescent microorganisms and microbial reaction vessel for the device {Bioluminescence Measuring Apparatus and the Vial Holder}

1 is a perspective view of an automatic water toxicity measurement device according to the present invention

2 is a perspective view of a light receiving sensing device according to the present invention;

3 is a side view of a light receiving sensing device according to the present invention;

4 is a plan view of a light receiving sensing device according to the present invention (with one cam not mounted)

5 is a plan view of a light receiving sensing device according to the present invention (with two cams mounted)

6 is a perspective view showing a form in which the reaction vessel according to the present invention is connected

7 is a plan view showing a form in which the reaction vessel according to the present invention is connected

8 is a plan view of the main part of the water toxicity measurement apparatus according to the present invention

9 is a perspective view of main parts of the water toxicity measurement apparatus according to the present invention

10 is a connection state diagram of a light receiving sensing device and a reaction vessel according to the present invention.

11 is a perspective view of a reaction vessel separately according to the present invention

12 to 15 is a plan view showing the operation state by order of the light receiving sensing device according to the present invention

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※ Explanation of main drawing code

2: reaction vessel 4: vial

6: reaction vessel transfer device 8: light receiving sensing device

10: power unit 12: sprocket

14: light receiving sensor 16: bar

18: vertical reciprocating member 19: horizontal reciprocating member
20: hinge coupling 22: gear train

23: roller bearing 24: cam

26: compression spring 28: vertical sealing

30: side seal 32: injection hole

34: measuring hole 36: pin-shaped ring

38: ring connection hole 40: vertical shield

41: side shield 42: reaction vessel push

43: compression spring 44: O-ring
45: drive motor 46: drive motor
47: roller bearing 48: light receiving sensor holder
49: reciprocating member holder 50: guide pin
51: guide pin groove A: raw water supply position
B: Microbial feeding position C: Measuring position

The present invention relates to an automatic water toxicity measurement device for automatically measuring water toxicity using subsea luminescent microorganisms, and more specifically, periodically supplying a large amount of test containers while keeping them at a constant temperature in a measuring device, It is used for automatic measurement of water toxicity using luminescent microorganisms that can measure the toxicity of water periodically and continuously precisely for a certain period by unmanned operation at the water intake station while maintaining the inert temperature at low temperature. It relates to a microbial reaction vessel.

With the development of industry, the emission of heavy metals is increasing. Especially, since the water toxicity problem is the fastest and most seriously affecting daily life, the toxic status of water sources directly related to the natural ecosystem is continuously measured and analyzed. There is a need for automatic water toxicity measurement equipment that can predict and manage dangerous situations.

Methods of measuring water toxicity include methods using living organisms, methods of chemical analysis, and methods of electro ionization analysis.

The method of measuring water toxicity using living organisms is faster, simpler and more economical than chemical analysis or electroionization. At present, the method of measuring water toxicity at most domestic water intake stations is to cultivate certain fish or daphnia. This method requires a long time of 3 or 4 days to obtain a measurement result, and there is a problem that it takes too much time to treat water that has already entered the water treatment plant.
One method of measuring water toxicity using living organisms is to use luminescent microorganisms, which are bioluminescence within a short time depending on the type and content of toxic substances when the luminescent microorganism comes into contact with a specific toxic substance. The reduction pattern of) is to use different characteristics as the measurement principle. In other words, by containing a certain amount of luminescent microorganisms in the raw water to be tested for water quality and measuring the change in the amount of luminescence while maintaining the specific temperature, the luminescent microorganism can quickly and accurately measure the presence and toxicity of toxic substances. It is to use as a sensor. This method measures changes in the amount of emitted light by luminescent microorganisms, and not only has excellent sensitivity to the reaction, but also has a characteristic that the reaction is sensitive to very small amounts of toxic substances within a short time. There are advantages to it.

Photobacterium phosphoreum, one of the submarine luminescent microorganisms, is capable of growing at low temperatures, and immobilized in a gel state for relatively long term storage, especially at 4 ° C, and rapidly activated at room temperature. It can be used to measure the state of water toxicity in a short time, and researches on the characteristics of microbial culture, long-term storage, and response to water toxicity have been actively conducted. However, most studies are limited to the study of measuring the luminescence properties of luminescent microorganisms by a manual batch method, and commercialization as an equipment for continuously measuring and monitoring water toxicity is not realized.

Automation of water toxicity measurement equipment using luminescent microorganisms is urgently required for the rapid, accurate and economical measurement of water toxicity, especially in unmanned waterworks intake stations. Therefore, it is necessary to develop an automatic measuring device capable of real-time monitoring by measuring water toxicity for a certain period of time periodically and continuously even in an unattended state using a luminescent microorganism.

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The study of the cultivation and measuring apparatus of luminescent microorganisms is a special field that is developed only in some developed countries such as the United States and Europe. In some developed countries, research on the measurement of water toxicity using luminescent microorganisms is in the practical stage, and furthermore, it has succeeded in miniaturization and development of equipment that can easily measure the water toxicity anywhere, anytime. However, the batch method of adding the active solution to most lyophilized microorganisms has difficulty in principle in automated measurement.

Typical products commercialized include Merck Ltd., USA. Co.'s ToxAlert. It is a freeze-dried microorganism of the seabed luminescent microorganisms, which enables easy and rapid measurement of water toxicity.It can be measured at various temperature ranges between 0 ° C and 30 ° C, and several water samples can be measured simultaneously. It is easy to use and can be used for on-line measurement as well as for toxicological analysis and analysis of sample water. However, measurement devices using lyophilized seabed microorganisms, such as Microtox, which have been widely known so far, have been commercialized for measurement by batch methods, so it is difficult to continuously and automatically monitor the state of water toxicity. In addition, since it uses the microorganisms as it is, it is necessary to supply an additional special solution in order to have the best activity to check the degree of toxicity, and there is a problem in the reproducibility of the water quality analysis result because the measurement variation is very severe.

In addition, the batch method, which is a method of culturing microorganisms and storing them for a long time, measures water toxicity by a manual one-time method, so that the qualitative and quantitative analysis of the measurement results is not reliable.

Korean Patent Application No. 10-2004-88687 `` Automatic Early Warning System for Water Pollution Using Microorganisms and Automatic Early Warning System '' is a gel for long-term preservation of Photobacterium phosphoreum. Regarding the culture and storage technology of the microorganism to be treated,

A vial rotation moving part installed horizontally every predetermined time and having a plurality of vial mounting holes in which the vial is installed along the rotation direction; An automatic vial feeder configured to rotate horizontally at a predetermined time and having a plurality of vial mounting holes formed therein, and a carriage for reciprocating between the swivel and the vial rotation moving part and transferring the vial; A microorganism quantitative supply device having a microbial outlet on the vial mounting hole of the vial rotation moving unit; A constant temperature box connected to the microorganism inlet port of the microorganism metering device; A sample dosing device having a sample supply nozzle above the vial mounting hole of the vial rotation moving part; A light receiving sensor disposed below the vial rotation moving part at a position of the sample supply nozzle; The automatic vial feeder, the vial rotary mover, the microbial quantitative feeder, the sample quantitative feeder, and a calculation controller connected to the light receiving sensor, the automatic vial feeder along the rotational opposite direction of the vial rotary mover And a microbial metering device and a sample metering device are arranged in order, and relates to the structure of the device to enable the unmanned operation by automating the entire process from the supply of the vial to the measurement and discharge.

Analyze and process the light emission measured by the optical sensor, save and print the measurement data, remote control the image processing technology and measurement equipment to display the measurement results on the computer monitor screen and LED display Technology for transmitting and receiving. Although a number of vials required for measurement for a certain period are pre-inserted into the vial mounting holes of the rotary table, the reciprocating vial feeder automatically supplies the vials every time the measurement is performed. Due to the structure of the carriage that transfers the vials and reciprocates between the rotating parts of the vial and the vial, the entire measuring system becomes quite large in the size of an office large cabinet. Due to the inaccuracy of the quantitative injection of the feeder supplying the light emitting microorganisms, it is difficult to realize the automation of the water toxicity measurement equipment. In addition, due to the lack of a light blocking structure that blocks external light flowing into the vial being measured, the entire interior of the measuring device including the low temperature storage container, the vial storage swivel, and the vial transfer carriage, etc. Due to the difficulty in the process and the reason for this, there is a problem that the instrument cannot be opened during the measurement operation, and the measurement should be stopped during the microbial and vial supply.

The present invention is a microorganism culture and long-term storage technology for gelling small-sized gel to treat photobacterium phosphoreum for a long time storage, the light emission characteristics of the light-emitting microorganisms for a specific specific toxic substance Using a combination of a measurement database, image processing technology of measured light emission, remote control of measuring equipment, and data transmission / reception technology, there is a need for a mechanical device that continuously measures water toxicity continuously for a certain period of time at an unmanned site such as a water intake station of a water supply source. do.

The present invention for realizing this object is a device for periodically and continuously supplying the vial used for the measurement to automatically measure the water toxicity using the light-emitting microorganisms at constant temperature, and the gelled light-emitting microorganisms compactly It is to provide an automatic water quality toxicity measurement device using a luminescent microorganism to periodically quantitatively inject the luminescent microorganisms into the reaction vessel while maintaining a low temperature in the inactive state, and quantitatively inject the raw water to be measured.

For this purpose, a shielding part is provided to block the light in the reaction vessel so that external light does not affect the emission of light emitting microorganisms that emit minute light in the vial when measuring water quality. Automatically shield the reaction vessel so that the inlet is blocked, the reaction vessel functions only as a separate dark room when measuring, the structure in which a plurality of reaction vessels connected in a chain shape; Storing a plurality of connected reaction vessels at constant temperature; Periodic and continuous automatic transfer device; Equipment for continuous measurement without interruption of measurement work by vial replacement and supply of light emitting microorganisms during measurement; An apparatus for automatically quantitatively injecting a vial into a vial while maintaining a luminescent microorganism at a low temperature, and periodically quantitatively injecting raw water to be tested into the vial; In addition, there is a need for a photodetection system for measuring and analyzing changes in the amount of emitted light of luminescent microorganisms to determine the toxic state of water quality and storing the measurement data.

Technical features for achieving the intended purpose of the present invention through the reaction vessel transfer device 6, the light receiving sensing device 8, the drive motor 45, 46 the reaction vessel transfer device 6 and the Water poisoning automatic measuring device and reaction vessel consisting of a power unit 10 for driving the light receiving sensing device 8, a controller connected to the light receiving sensing device 8, a device for automatically injecting the light emitting microorganisms and raw water for water quality inspection (2) ),

The reaction vessel transfer apparatus 6 includes a plurality of reaction vessels 2 connected in a chain shape by a pair of rings, a plurality of sprockets 12 carrying the plurality of reaction vessels 2 and the sprockets 12. It consists of a plurality of roller bearings 47 and smoothing the rotation of the drive motor 45 for driving the sprocket 12,

 Inside the cylinder of each of the plurality of reaction vessels (2) put a pair (4) to form a pair,

The light receiving sensing device 8 includes a light receiving sensor 14 for detecting the amount of light emitted by the light emitting microorganisms, a light receiving sensor holder 48 on which the light receiving sensor 14 is mounted, and a vertical reciprocating shielding the reaction container 2 vertically. The member 18, the horizontal reciprocating member 19 for pushing the reaction vessel 2 horizontally to shield the side of the reaction vessel 2, the hinge coupling 20 to the two reciprocating members 18, 19 A plurality of bars 16 and roller bearings 23 in contact with the cams 24 to transfer the displacement of the cams 24 to the bars 16 and the bars 16 in a lateral direction. Side sealing of the two cams 24, the gear train 22 for driving the cam 24, the drive motor 46 for driving the gear train 22, and the light receiving sensor holder 48. The reaction vessel is composed of a reaction vessel pusher 42 and the compression spring 43 to push the reaction vessel 2 to be separated from the 30,

In another characteristic, the vertical reciprocating member 18 and the horizontal reciprocating member 19 are driven by the gear train 22 by one driving motor 46,

The vertical reciprocating member 18 and the horizontal reciprocating member 19 is configured to be automatically returned by the compression spring 26, respectively,

The cam 24 is an asymmetrical oval of the short side is to be in contact with the bar 16 is moved in the order of left and right and up and down,

The vertical reciprocating member 18 is formed in the lower portion of the vertical reciprocating member 28 so as to be a dark room, the structure of the light inlet is completely blocked by the O-ring 44 mounted in a circular groove when in close contact,

When the vertical reciprocating member 18 is in close contact with the upper portion of the reaction vessel (2), the light is completely blocked so that the vertical shield 40 is formed so that the inside of the reaction vessel (2), the dark room,

Shape of the reaction vessel (2) of the portion where the reaction vessel (2) and the light receiving sensor 14 is connected is that the side shield portion 41 is formed on the side so that light is blocked to become a dark room,

When the horizontal reciprocating member 19 is moved to the left by the action of the cam 24, a pair of upper and lower reaction vessel push rods 42 which are under the force of the compression spring 43 mounted in the light receiving sensor holder 48 inner groove are provided. Is configured to push the reaction vessel (2) a certain distance from the side sealing portion 30,
The other ends of the vertical reciprocating member 18 and the horizontal reciprocating member 19 of the bar 16 are connected to each other by two bars 16 perpendicular to each other on one axis and hinged together 20. Doing,

The gear train 22 includes one drive gear 22a directly connected to the drive motor 46 shaft, one idling gear 22b for transmitting the drive of the drive gear 22a, and two contacting the idling gear 22b. Having two driven gears 22c therebetween,

The plurality of sprockets 12 is 10 to 15, the roller bearings 47 are mounted one by one above and below each of the sprocket 12 rotation axis to facilitate the rotation of the sprocket 12,

The reaction vessel 2 into which the vials 4 are put one by one is 130 to 240 in consideration of the continuous measurement period.

Another feature is

Water injection device using a light emitting microorganism, characterized in that the injection port 32, the measuring hole 34 and two rings 36, 38 are provided on the cylindrical upper portion of the reaction vessel (2), respectively Providing a reaction vessel (2) for use in,

One of the ring is a pin ring 36, the other ring ring hole 38 is formed to be connected to each other in a chain shape with the pin ring 36 of the other reaction vessel (2),

The injection hole 32 and the measuring hole 34 has a vertical shield 40 to block the light,

The pin-shaped ring 36 and the ring connecting hole 38 can be fastened and separated, and bent freely in a connected state.

Hereinafter, a configuration according to the present invention will be described in detail with reference to the accompanying drawings.

1 is an overall perspective view of an automatic water toxicity measurement apparatus according to the present invention for driving the reaction vessel transfer device 6, the light receiving sensing device 8 and the reaction vessel transporting device 6 and the light receiving sensing device (8) The power unit 10 is shown, and the controller connected to the light receiving sensing device 8, the device for automatically injecting the raw water for the inspection of light microorganisms and water quality inspection can be used in the prior art, so the description is omitted in the drawings. .

8 and 9, the main portion of the automatic water toxicity measurement device in a chain shape by the reaction vessel transfer device 6, the light receiving sensor 14, the drive motor 45 and 46, the connecting ring A plurality of reaction vessels 2 to be connected, consisting of a plurality of sprockets 12 and roller bearings 47 for conveying the reaction vessels 2, the number of sprockets 12 and the number of reaction vessels 2 You can do it differently depending on its placement. In consideration of the continuous automatic measurement period according to the maximum survival time of the solidified luminescent microorganism, the plurality of sprockets 12 is preferably 10 to 15 and the reaction vessel 2 is preferably 130 to 240.

4 and 5, the light receiving sensing device 8 includes a light receiving sensor 14 for detecting the light emission amount of the light emitting microorganism, a light receiving sensor holder 48 on which the light receiving sensor 14 is mounted, and the reaction vessel ( 2) the vertical reciprocating member 18 and the O-ring 44 to seal the top to the bottom, the horizontal reciprocating member 19 for pushing the reaction vessel 2 horizontally for side sealing, the reciprocating member 18, ( 19, a plurality of bars 16 hinged to 20, roller bearings 23 in contact with the cam 24 to transfer the displacement of the cam 24 to the plurality of bars 16, the bars The two cams 24 for moving the side 16, the gear train 22 for driving the cams 24, and the reaction vessel 2 are pushed out to separate from the side seal 30. The reaction vessel pusher 42, the compression spring 43 is always applying a force to the reaction vessel pusher 42, the reciprocating member 18, 19 is a reciprocating member hole Carried by 49, it is a reciprocating movement by the interaction of a compression spring 26 provided in the bar 16 and the reciprocating member holder 49.

 At this time, the horizontal reciprocating member 19 reacts so that the side shield portion 41 of the reaction container 2 is in close contact with the side sealing portion 30 of the light receiving sensor holder 48 on which the light receiving sensor 14 is mounted. The container 2 is moved to the right side, and the guide pins 50 are provided on the upper and lower center axes of the horizontal reciprocating member 19, respectively, to move along the upper and lower guide pin grooves 51 in the reciprocating member holder 49. It is a structure that prevents rotation. The reaction vessel pusher 42, which is installed in the groove provided in the light receiving sensor holder 48 and is subjected to the force of the compression spring 43, has the reaction vessel 2 when the horizontal reciprocating member 19 moves to the left by the action of the cam 24. ) And the side shield 41 and the light receiving sensor holder 48 of the reaction container 2 when the reaction container 2 is transferred one by one as the sprocket 12 is rotated afterwards. The side seals 30 are to be kept away from each other without touching. The vertical reciprocating member 18 is for sealing the upper portion of the reaction vessel 2 by bringing the vertical sealing portion 28 into close contact with the vertical shielding portion 40 provided on the upper portion of the reaction vessel 2.

The power unit 10 shown in the lower part of Figure 1 may be installed in consideration of the size and installation space of the device, but in order to minimize the size is preferably set to one.

The reciprocating members 18 and 19 are configured to be automatically returned by the compression spring 26, but the reaction with the O-ring 44 installed in the vertical groove 28 circular groove of the end of the vertical reciprocating member 18. The vertical shielding portion 40 provided on the upper portion of the container 2 is sufficiently in close contact with each other to completely block light, and the horizontal reciprocating member 19 has a side shielding portion 41 of the reaction vessel 2 having a light receiving sensor holder ( The O-ring 44 installed in the circular groove of the side sealing part 30 of the 48 and the side shielding part 41 of the reaction container 2 are sufficiently in contact with each other so that the light is completely blocked so that the compression is performed in consideration of the configuration or operating efficiency of the dark room. The tension of the spring 26 is selected.

4, 5 and 12 to 15, the cam 24 is asymmetrical oval short side is to be in contact with the bar 16 to move in the order of left and right and vertical direction, wherein the The gear train 22 attached to the cam 24 always rotates in the same direction.

Referring to FIG. 4, the gear train 22 transmits one driving gear 22a directly connected to the driving motor and one driving gear 22c for driving the driving gear 22a to two driven gears 22c. 22b and two driven gears 22c which are integrally rotated with each cam 24 to drive the cam 24 and are in contact with the idling gear 22b, and have a cam 24 therebetween. The drive of the contact with the roller bearing 23 is transmitted to the bar (16).

 In FIG. 4, when the horizontal reciprocating member 19 moves to the left by the action of the cam 24, the pair of upper and lower reaction vessel push rods 42 receiving the expansion force of the compression spring 43 mounted therein is the side sealing part. It is pushed to the left to separate the reaction vessel (2) from (30). In FIG. 4, the reaction vessel pusher 41a shows a position where the reaction vessel 2 is in close contact with the side seal 30, and the reaction vessel pusher 41b has the reaction vessel 2 in the side seal 30. It is the position where it was pushed out of the and separated.

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Referring to FIGS. 6 and 11, the reaction vessel 2 will be described. A vial 4 is placed in the reaction vessel 2, the inlet 32 is disposed on the upper portion of the cylinder, and the measuring sphere 34 is disposed on the side. Rings 36, 38. At the ends of the injection hole 32 and the measuring hole 34, a vertical shielding portion 40 and a side shielding portion 41 are respectively provided to block light. One of the rings is a pin-shaped ring 36, the other is a ring connecting hole 38 is formed to be connected to each other in a chain shape with the pin-shaped ring 36 of the other reaction vessel (2), the pin-shaped ring ( The chain 36 and the ring connecting hole 38 connected to each other are configured to be bent freely. In particular, the lower portion of the ring connecting hole 38 of the reaction vessel (2) may have an additional ring of a part of the circular ring is separated in order to be firmly fixed in the form of a chain. As shown in FIG. 13, it is important that the reaction vessel 2 is manufactured so as not to be a problem when transferring between the horizontal reciprocating member 19 and the light receiving sensor holder 48.
Referring to FIG. 12, the front surface of the light receiving sensor holder 48 on which the light receiving sensor 14 is mounted has a side sealing part 30 formed thereon, and the measuring hole 34 located on the side of the reaction container 2 is provided. ) Is positioned in front of the light receiving sensor 14 and then the horizontal reciprocating member 19 is moved to the right side so that the side shield 41 of the side of the reaction vessel 2 is attached to the O-ring 44 mounted to the side seal 30. When the light is closed, the light is blocked, and when the vertical reciprocating member 18 is moved downward, the O-ring 44 mounted on the vertical sealing portion 28 below the vertical reciprocating member 18 and the vertical provided on the reaction vessel 2 are vertical. Since the shield 40 is in close contact with the light, the inside of the reaction vessel 2 becomes a complete dark room. And the other end portion that is not connected to the bar 16 and the reciprocating member (18, 19) is in contact with each other at right angles to the hinge coupling 20 on one axis, so that one end of the bar (16) The hinge couplings 20 and the other ends of the reciprocating members 18 and 19 are hinged 20 to each other.

Hereinafter, the operation according to the present invention will be described.

The operation step-by-step view of the light receiving sensing device 8 in the sequence according to the operation is shown in FIGS. 12 to 15.

Raw water supply position (A), microorganism supply position (B) and measurement position (C) of the reaction vessel (2) in which the measurement target vial (4) is placed in the operation of the reaction vessel transfer device (6) for water quality measurement. ) Is shown in FIG. 10. Among the plurality of reaction vessels 2 connected in a chain shape and each of which vials 4 are put one at a time and kept at a constant temperature, the raw water supply position A next to the second position from the front position of the light receiving sensor 14. After supplying a certain amount of raw water automatically or manually at a predetermined time, and passing a predetermined time so that the temperature of the supplied raw water is suitable for activation of the light-emitting microorganism and the temperature suitable for measurement, the reaction vessel transfer device 6 is driven to receive the light receiving sensor ( Transfer the reaction vessel 2 one space in the direction of 14).

After emitting a predetermined amount of the light-emitting microorganisms stored at a low temperature at a microorganism supply position (B) with a known microorganism metering device, and after a predetermined time to reach a temperature suitable for the activation and measurement of the light-emitting microorganisms, the reaction vessel transfer device (6) Drive the reaction vessel 2 one space to the measurement position (C) in front of the light receiving sensor (14).

When the position of the reaction vessel 2 to be measured is moved to the measurement position C (FIG. 12), the drive motor 46 of the light receiving sensing device 8 is driven to rotate the gear train 22 and the cam ( 24 is operated to sequentially move the side bar 16 to the horizontal reciprocating member 19 to the right to close the reaction vessel 2 to the light receiving sensor 14 (Fig. 13), the vertical bar ( 16) the vertical reciprocating member 18 is lowered by moving (Fig. 14). At this time, by the expansion force of the compression spring 26, the vertical sealing portion of the side shield portion 41 and the vertical reciprocating member 18 of the side shield portion 41 and the light receiving sensor holder 48 of the reaction container 2 in sequence. (28) and the vertical shield 40 of the reaction vessel (2) is in close contact with the O-ring 44 fitted in the circular groove, respectively, so that the light flowing into the reaction vessel (2) is completely blocked.

The amount of light emitted decreases as the light microorganisms mixed with the raw water to be tested in the vial (4) in the reaction container (2) in the dark room are completely blocked due to the toxicity of the raw water. Is continuously measured by the light receiving sensor 14, and converted into a digital value, and the value measured within the initial predetermined time is set as a reference value, and the subsequent light emission value is recorded as a normalized value. At this time, the measured data processed by the controller connected to the light receiving sensor 14 is compared with the measurement values for the standard heavy metal-containing water quality previously inputted in the computer database to determine the type of toxicity and the degree of toxicity.

After the measurement of the change in the light emission amount of a certain time is completed, the gear train 22 is rotated by the rotation of the drive motor 46 of the light receiving sensing device 8 to operate the cam 24 and the compression spring 26 While further compressing, the vertical reciprocating member 18 is sequentially moved upward to separate the vertical shield portion 40 of the reaction vessel 2 (FIG. 15), and the horizontal reciprocating member 19 is moved leftward. At the same time, the reaction vessel pusher 42 pushes the reaction vessel 2 to the right, thereby releasing the side shield portion 41 free from the closed state, so that the reaction vessel 2 having finished the measurement is placed in front of the light receiving sensor 14. It becomes the state which can be moved to the side at the position of (FIG. 12).

Thereafter, when the sprocket 12 is rotated by operating the driving motor 45, the reaction vessel transfer device 6 is operated so that the reaction vessel 2 in which the measurement is completed is moved to the side and exits, and the next turn is connected. That is, the next connection reaction vessel (B), which is waiting for the raw water to be measured and the light emitting microorganism to be pre-injected and raised to the activation temperature of the light emitting microorganism, is moved to the measurement position (C) in front of the light receiving sensor 14 to continuously measure water toxicity. This is done.

After that, the operation is repeated according to the above-described sequence, and the automatic measurement of water toxicity using the luminescent microorganism is continuously performed.

Therefore, the automatic measurement device for water toxicity using the light-emitting microorganism according to the present invention has a large size of a conventional cabinet for offices, followed by many parts of the conventional equipment, and complicated and complicated operation of the equipment. It can be miniaturized to a small size that can be carried.
It is a structure and operation that simply moves the reaction vessel (2), which is connected in a chain shape by inserting a vial (4), side by side, so that it can operate correctly without problems in supplying the vials periodically and continuously. have.
In addition, the conventional measuring system had a problem of darkening the entire interior, but the present invention consists of a structure in which only the reaction vessel (2) in which the vial (4) placed in the measuring state is instantaneously sealed independent dark room. The microbial supply and vial (4) can be replaced even during the water quality measurement work, so that the measurement can be continuously performed without interruption.

Claims (15)

Power unit 10 for driving the reaction vessel transfer device 6 and the light receiving sensing device 8 through the reaction vessel transfer device 6, the light receiving sensing device 8, the driving motor 45, 46. In the water quality toxicity automatic measuring device consisting of a controller connected to the light receiving sensing device (8), a device for automatically injecting raw water for water quality inspection and The reaction vessel transfer apparatus 6 includes a plurality of reaction vessels 2 connected in a chain shape by a pair of rings, a plurality of sprockets 12 carrying the plurality of reaction vessels 2 and the sprockets 12. It consists of a plurality of roller bearings 47 and a drive motor 45 for driving the sprocket 12 for the purpose of smoothly rotating, Inside the cylinder of each of the plurality of reaction vessels (2) put a pair (4) to form a pair, The light receiving sensing device 8 includes a light receiving sensor 14 for detecting the amount of light emitted by the light emitting microorganisms, a vertical reciprocating member 18 for vertically shielding the reaction vessel 2, and a reaction vessel 2 for side shielding. Horizontal reciprocating member 19 which pushes horizontally, a plurality of bars 16 hinged to the two reciprocating members 18, 19, displacement of the cam 24 to the plurality of bars 16 Roller bearing 23 in contact with the cam 24, two cams 24 for moving the bar 16 in the lateral direction, and a gear train for driving the cams 24 to deliver the (22), the drive motor 46 for driving the gear train 22, the reaction vessel pusher 42 to push out a predetermined distance so that the reaction vessel 2 can be separated from the side seal (30) , The foot consisting of a compression spring 43 for applying a force that always pushes the reaction vessel pusher 42 in the direction of the reaction vessel (2) Automatic water toxicity measuring apparatus using microorganisms The method of claim 1, wherein the vertical reciprocating member 18 and the horizontal reciprocating member 19 is driven by one drive motor 46 by the gear train 22, water quality toxicity using light-emitting microorganisms Measuring device The apparatus of claim 1, wherein the vertical reciprocating member 18 and the horizontal reciprocating member 19 are configured to be automatically returned by the compression spring 26. The method of claim 1, wherein the cam 24 has a short-sided asymmetrical oval shape, the bar 16 is in contact with the water quality automatic toxicity measurement device using a light-emitting microorganism, characterized in that the movement in the order of left and right and vertical direction. The method according to claim 1, wherein the front surface of the light receiving sensor holder 48 on which the light receiving sensor 14 is mounted, the side sealing portion 30 is formed so that the reaction vessel 2 becomes a dark room using a light emitting microorganism Water Toxicity Automatic Measuring Device The horizontal reciprocating member 19 of the light receiving sensing device 8, when moved to the left by the action of the cam 24, is applied to the force of the compression spring 43 mounted in the hole provided in the light receiving sensor holder 48. Reaction container pusher 42 which pushes the reaction container 2 from the side-sealing part 30 of the light receiving sensor holder 48 by the pair of reaction container pushers 42 which are received up and down is provided in a predetermined distance. Water Toxicity Automatic Measurement Device Using Luminescent Microorganisms The method of claim 1, wherein the vertical reciprocating member 18 is a watertight toxicity automatic measurement device using a light emitting microorganism, characterized in that the vertical sealing portion 28 is formed to form a dark room. The method of claim 1, wherein the other end portion of the bar 16, which is not connected to the reciprocating members 18 and 19, is hinged to the two bars 16 in a hinge at a right angle to each other. Automatic water toxicity measurement device using a light-emitting microorganism, characterized in that 2. The gear train 22 of claim 1, wherein the gear train 22 is integrally formed with one drive gear 22a directly connected to the drive motor, one idling gear 22b and a cam 24 that transmit driving of the drive gear 22a. Automatic measurement device for water toxicity using a light emitting microorganism, characterized in that it has two driven gears (22c) between the driving gear and the idling gear (22b) in between. The method of claim 1, wherein the plurality of sprockets 12 is 10 to 15 water quality toxicity automatic measurement device using a light-emitting microorganism, characterized in that The method of claim 1, wherein the reaction vessel (2) is an automatic water quality toxicity measurement device using a light emitting microorganism, characterized in that 130 to 240 Reaction vessel (2) used for the automatic measurement device for water toxicity using a light-emitting microorganism, characterized in that it has an injection hole 32, a measuring hole 34 and two rings 36, 38 on the side of the cylindrical upper portion The method of claim 12, wherein one of the ring is a pin ring 36 and the other ring ring hole 38 is formed to be connected to each other and connected in a chain shape with the pin ring 36 of the other reaction vessel (2) Reaction vessel used for automatic measurement of water toxicity using luminescent microorganisms (2) The method of claim 12, wherein the injection hole 32 and the measuring hole 34 has a vertical shielding portion 40 and the side shielding portion 41 to block the light, respectively, characterized in that the automatic measurement of water toxicity using light-emitting microorganisms Reaction vessels used for (2) The reaction vessel (2) of claim 12, wherein the chain in which the pin-shaped ring (36) and the ring connecting hole (38) are connected to each other is bent freely.

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