KR100663038B1 - 외부유전자의 발현 및 단백질의 분비를 위한 벡터 pMSG1220 - Google Patents
외부유전자의 발현 및 단백질의 분비를 위한 벡터 pMSG1220 Download PDFInfo
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- KR100663038B1 KR100663038B1 KR1020040057021A KR20040057021A KR100663038B1 KR 100663038 B1 KR100663038 B1 KR 100663038B1 KR 1020040057021 A KR1020040057021 A KR 1020040057021A KR 20040057021 A KR20040057021 A KR 20040057021A KR 100663038 B1 KR100663038 B1 KR 100663038B1
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Abstract
Description
균주 (Strain) | 종 (Species) | 감염타입 | β-락탐의 최소억제농도(MICs)(mg/L) | ||||||||||
AMX | AUG | LOT | TAX | TAZ | ATM | FEP | IMP | MER | FOX | TTN | |||
K9973 | E. cloacae | UTI | >256 | 128 | >256 | 32 | 256 | 256 | 4 | 0.25 | 0.25 | 256 | 256 |
K99230 | E. cloacae | Pneumonia | >256 | 256 | >256 | 32 | 128 | 128 | 2 | 0.5 | 0.125 | 128 | 256 |
K992004.1 | E. cloacae | Wound infection | >256 | 256 | >256 | 16 | 256 | 256 | 4 | 0.25 | 0.125 | 256 | 256 |
K995120.1 | E. cloacae | Wound infection | >256 | 128 | >256 | 32 | 128 | 128 | 2 | 1 | 0.125 | 256 | 256 |
K9911729 | E. aerogenes | Pneumonia | >256 | >256 | >256 | 32 | 64 | 128 | 1 | 0.5 | 0.25 | >256 | 128 |
K9914325 | E. cloacae | UTI | >256 | 128 | >256 | 32 | 128 | 128 | 4 | 0.5 | 0.125 | 128 | 64 |
[주] 1. AMX는 아목시실린, AUG는 아목시실린-클라불라닉산, LOT는 세팔로틴, TAX는 세폭탁심(cefotaxime), TAZ는 세프타지딤, ATM은 아즈트레오남, FEP는 세페핌(cefepime), IMP는 이미페넴(imipenem), MER은 메로페넴(meropenem), FOX는 세폭시틴, TTN은 세포테탄을 의미함. 2. UTI는 요도감염, Pneumonia는 폐렴을 통한 감염, Wound infecion은 상처를 통한 감염을 의미함. |
β-락타메이즈 | 출처(기탁번호; Accession number) | 비고 |
EcloQ908R | 엔테로벡터 클로아케(Enterobacter cloacae) Q908R (X08081) | 염색체성 |
EcloMHN1 | 엔테로벡터 클로아케(Enterobacter cloacae) MHN1 (X08082) | 염색체성 |
EcloCHE | 엔테로벡터 클로아케(Enterobacter cloacae) CHE (AJ278994) | 염색체성 |
EcloGC1 | 엔테로벡터 클로아케(Enterobacter cloacae) GC1 (D44479) | 염색체성 |
EcloGN747 | 엔테로벡터 클로아케(Enterobacter cloacae) GN747 (AB016611) | 염색체성 |
EcloOUDhy | 엔테로벡터 클로아케(Enterobacter cloacae) OUDhy (AJ278995) | 염색체성 |
EcloP99 | 엔테로벡터 클로아케(Enterobacter cloacae) P99 (X07274) | 염색체성 |
Eare1 | 엔테로박터 아에로지너스(Enterobacter aerogenes) (AF211348) | 염색체성 |
EcloK992004.1 | 엔테로벡터 클로아케(Enterobacter cloacae) K992004.1 (AF411144) | 염색체성 |
EcloK995120.1 | 엔테로벡터 클로아케(Enterobacter cloacae) K995120.1 (AF411145) | 염색체성 |
EcloK99230 | 엔테로벡터 클로아케(Enterobacter cloacae) K99230 (AF411146) | 염색체성 |
EcloK9911729 | 엔테로벡터 클로아케(Enterobacter cloacae) K9911729 (AF411147) | 염색체성 |
EcloK9973 | 엔테로벡터 클로아케(Enterobacter cloacae) K9973 (AF411148) | 염색체성 |
EcloK9914325 | 엔테로벡터 클로아케(Enterobacter cloacae) K9914325 (AF411149) | 염색체성 |
MIR-1 | 클레브시엘라 퓨모니에(Klebsiella pneumoniae) (M37839) | 플라스미드성(Plasmid-encoded) |
ACT-1 | 클레브시엘라 퓨모니에(Klebsiella pneumoniae) MCQ-95 (U58495) | 플라스미드성(Plasmid-encoded) |
프라이머 (Primer) | 서열(Sequence) | 뉴클레오티드 위치 (Nucleotide position) | 증폭 크기(size) (bp) |
AmpCF1 | 5'-TCGGAATTCCGGAGGATTACTGATGATGA-3 ' | 1 | 1165 |
AmpCR1 | 5'-TTAGTCGACAATGTTTTACTGTAGCGCCTCG-3' | 1165 | |
A1 | 5'-CTATAAGTAAAACCTTCACCGG-3 ' | 264 | 871 |
A2 | 5'-TATGCCGCCTCAACGCGTG-3' | 1134 | |
A3 | 5'-TGCGCTTTTATCAAAACTGGCA-3' | 468 | 586 |
A4 | 5'-GCCACGTAGCTGCCAAACC-3' | 1053 | |
C-NdeIF | 5'-GTAGACCATATGCAACAACGACAATCCATCCTGTGG-3' | 1329 | 1179 |
N-XhoIB | 5'-GAATGTCTCGAGCTCTTTCTTTCAACCGGCCAAC-3' | 151 | |
Up-26b | 5'-CTATCATGCCATACCCGCAAAG-3' | 1766 | 608 |
Sp-His-SacI | 5'-GGCCGCTGAGCTCatgatgatgatgatgatgatgatgatgatgGATGCTGGCATCCACCAC-3' | 1159 | |
EK-AmpC-F | 5'-CATAGCGGCGAGCTCCATATCgacgacgacgacaagACGCCAGTGTCAGAAAAAC-3' | 1227 | 1137 |
AmpC-HindIII | 5'-GCTAATGCGAAGCTTTTACTGTAGCGCCTC-3' | 2364 |
종(Species) | 균주 (Strain) | β-락탐의 최소억제농도(MICs)(mg/L) | ||||||||||
AMX | AUG | LOT | TAX | TAZ | ATM | FEP | IMP | MER | FOX | TTN | ||
trf K9973 | E. coli | 128 | 128 | >256 | 8 | 16 | 4 | 0.125 | 0.25 | <0.06 | 8 | 4 |
trf K99230 | E. coli | 128 | 128 | >256 | 32 | 16 | 8 | 0.25 | 0.5 | <0.06 | 16 | 8 |
trf K992004.1 | E. coli | 16 | 16 | 32 | 1 | 0.5 | 0.5 | 0.125 | 0.25 | <0.06 | 2 | 1 |
trf K995120.1 | E. coli | 16 | 16 | 32 | 1 | 0.5 | 0.5 | 0.125 | 0.25 | <0.06 | 2 | 1 |
trf K9911729 | E. coli | 128 | 128 | >256 | 32 | 32 | 16 | 0.25 | 0.5 | <0.06 | 16 | 16 |
trf K9914325 | E. coli | 64 | 64 | >256 | 16 | 16 | 4 | 0.25 | 0.25 | <0.06 | 8 | 4 |
pLysS | E. coli | 1 | 1 | 2 | <0.06 | 0.125 | <0.06 | 0.06 | 0.25 | <0.06 | <0.06 | <0.06 |
[주] 1. AMX는 아목시실린, AUG는 아목시실린-클라불라닉산, LOT는 세팔로틴, TAX는 세폭탁심(cefotaxime), TAZ는 세프타지딤, ATM은 아즈트레오남, FEP는 세페핌(cefepime), IMP는 이미페넴(imipenem), MER은 메로페넴(meropenem), FOX는 세폭시틴, TTN은 세포테탄을 의미함. |
Claims (1)
- 엔테로박터속(Enterobacter spp.) 유래의 AmpC β-락타메이즈(lactamase)를 인코딩하는 유전자를 발현시키고, 상기로부터 발현된 단백질을 분비하는 개열지도 도 1에 개시된 벡터 pMSG1220.
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US4820642A (en) | 1983-04-04 | 1989-04-11 | The Regents Of The University Of California | Amplified expression vector |
US6472205B1 (en) | 1995-03-20 | 2002-10-29 | The Regents Of The University Of California | Cytosolic forms for β-lactamase and uses thereof |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US4820642A (en) | 1983-04-04 | 1989-04-11 | The Regents Of The University Of California | Amplified expression vector |
US6472205B1 (en) | 1995-03-20 | 2002-10-29 | The Regents Of The University Of California | Cytosolic forms for β-lactamase and uses thereof |
Non-Patent Citations (4)
Title |
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1020040057021 - 658791 |
논문(1986) |
논문(1987) |
논문(2004) * |
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