KR100639950B1 - Functional food comprising the ordorless extract of Chongkukjang and preparation method thereof - Google Patents
Functional food comprising the ordorless extract of Chongkukjang and preparation method thereof Download PDFInfo
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- KR100639950B1 KR100639950B1 KR1020040065703A KR20040065703A KR100639950B1 KR 100639950 B1 KR100639950 B1 KR 100639950B1 KR 1020040065703 A KR1020040065703 A KR 1020040065703A KR 20040065703 A KR20040065703 A KR 20040065703A KR 100639950 B1 KR100639950 B1 KR 100639950B1
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- cheonggukjang
- extract
- hours
- fermentation
- acid
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Abstract
본 발명은 불쾌취가 제거된 청국장 추출물의 제조방법 및 이로부터 생산된 청국장 추출물을 함유하는 건강기능식품에 관한 것으로, 보다 상세하게는 청국장 제조시 건조된 증자대두에 바실러스 서브틸리스(Bacillus subtilis)와 맥반석을 첨가하여 발효시키는 공정을 포함하는 불쾌취가 제거된 청국장의 제조방법 및 이로부터 생산된 청국장 추출물을 함유하는 건강기능식품에 관한 것이다. The present invention relates to a manufacturing method of the cheonggukjang extract from which the odor is removed and to a health functional food containing the cheonggukjang extract produced therefrom, and more specifically, Bacillus subtilis ( Bacillus subtilis ) It relates to a health functional food containing the cheongukjang extract produced therefrom and the unpleasant odor is removed, including the step of fermentation with the addition of ganbanseok.
본 발명의 제조방법에 의해 제조된 청국장은 전통적인 청국장의 맛이 재현됨과 동시에, 청국장 특유의 강한 불쾌취가 효과적으로 감소되고 풍부한 영양과 풍미를 나타내므로 영유아의 성장발달에 유용한 발효 이유식 및 건강보조식품에 이용될 수 있다. Cheonggukjang prepared by the manufacturing method of the present invention, while the taste of traditional Cheonggukjang is reproducible, the strong discomfort unique to Cheonggukjang is effectively reduced and rich nutrition and flavor are shown. Can be used.
청국장, 맥반석, 이유식Cheonggukjang, Macvanseok, Baby food
Description
본 발명은 불쾌취가 제거된 청국장 추출물의 제조방법 및 이로부터 생산된 청국장 추출물을 함유하는 건강기능식품에 관한 것이다.The present invention relates to a method for producing a cheongukjang extract from which the odor is removed and a health functional food containing the cheongukjang extract produced therefrom.
청국장은 콩을 원료로 한 우리나라의 대표적인 발효식품으로서, 간장, 된장, 고추장 등과 함께 오늘날까지 상용되어 온 전통 장류 중의 하나이며, 곡류를 주식으로 하는 우리민족에게 결핍되기 쉬운 필수아미노산 및 지방산을 공급시켜 주는 식품으로서 중요한 역할을 담당하여 왔다. 청국장은 삶은 콩을 볏짚에 붙어있는 고초균(枯草菌)이라 부르는 바실러스 서브틸리스(Bacillus subtilis)를 이용하여 띄워만든 것으로서, 발효과정 중에 고초균이 생산하는 효소에 의해서 그 특유의 맛과 냄새를 내는 동시에, 원료콩의 당질과 단백질에서 유래된 레반형 프룩탄(levan form fructan)과 폴리글루타메이트(polyglutamate)의 혼합물질인 점질물을 다량 생 성하며, 이렇게 발효 제조된 청국장은 영양면에서 된장이나 고추장보다 단백질과 지방함량이 높은 양질의 콩 발효식품인 것이다.Cheonggukjang is one of Korea's representative fermented foods made from soybean. It is one of the traditional types of soy sauce, soy sauce, soybean paste, and red pepper paste that have been commonly used to this day. The state has played an important role as a food. Cheonggukjang is made by using Bacillus subtilis called Bacillus subtilis attached to rice straw, which gives its unique taste and smell by the enzyme produced by Bacillus subtilis during fermentation. In addition, it produces a large amount of viscous substance which is a mixture of levan form fructan and polyglutamate derived from raw sugar and protein derived from soybeans. It is a high quality fermented soybean with high content of fat.
최근, 많은 연구자들에 의하여 청국장 내에 포함된 활성성분 및 그 역할이 밝혀지고 있는 바, 청국장은 콩에서 기인된 이소플라본(isoflavone), 피트산(phytic acid), 사포닌(saponin), 트립신 저해제(trypsin inhibitor), xhzhvpfhftocopherol), 불포화지방산, 식이섬유, 올리고당, γ-글루타밀트랜스펩티다제(γ-glutamyltranspeptidase; γ-GTP) 등의 각종 생리활성물질, 항산화물질 및 혈전용해 효소를 다량 함유하고 있기 때문에 기능성 식품으로서 그 중요성이 재조명되고 있다. 그러나, 우리나라에서 청국장을 현대화시키는 데에는 뚜렷한 결실이 없었는 바, 국민소득이 향상되고 식생활과 주거문화가 서구화되면서, 발효과정과 조리시 야기되는 심한 불쾌취 때문에 청국장의 소비는 제한적이었으며, 청국장의 불쾌취가 대량생산이나 산업화에 단점에 되어 왔다.Recently, many researchers have revealed the active ingredient contained in Cheonggukjang and its role, so isoflavone (isoflavone), phytic acid, saponin, trypsin from soybean origin inhibitors, xhzhvpfhftocopherol), unsaturated fatty acids, dietary fiber, oligosaccharides, γ-glutamyltranspeptidase (γ-glutamyltranspeptidase; γ-GTP) Its importance is being reexamined as a functional food. However, there was no clear fruit in modernizing Cheonggukjang in Korea. As the national income improved and the diet and living culture became western, the consumption of Cheonggukjang was limited due to the severe odor caused by fermentation process and cooking. Has been a disadvantage in mass production and industrialization.
청국장의 불쾌취를 없애려는 종래의 노력은 대개 전통적인 청국장의 제조시 불쾌취를 감소시킬 수 있는 원료를 첨가시켜주는 방법을 사용하였는바, 구체적으로 대한민국 특허 제134681호에는 쑥추출물과 고추씨 기름을 첨가하여 이취를 제거시킨 청국장 제조방법이, 대한민국 특허공개 제1996-33292호에는 삼백초 뿌리를 이용하여 이취를 제거시킨 청국장 제조방법 및 대한민국 특허공개 제1997-64430호에는 콩의 마른 낟알을 단계적으로 가공하여 역겨운 냄새를 제거한 청국장의 제조방법에 개시된 바 있다. 그러나 상기한 방법으로 청국장을 제조시에는 청국장의 불쾌취를 어느정도 감소시키는 데에는 효과가 있으나, 청국장의 맛을 저해하는 이종원료의 사용으로 인하여, 전통적인 청국장의 맛이 발현되지 않으며, 식음시 이질감을 느끼게 되어, 기호도가 오히려 감소되는 단점 및 제조방법이 지나치게 복잡한 단점을 지니고 있다.Conventional efforts to eliminate the odor of Cheonggukjang have generally been made by adding raw materials that can reduce the odor in the manufacture of traditional Cheonggukjang. Specifically, Korean Patent No. 134381 adds mugwort extract and red pepper seed oil. Cheonggukjang manufacturing method to remove odor by using the Korean Patent Publication No. 1996-33292, Cheonggukjang manufacturing method to remove the odor using the three hundred seconds root and Korean Patent Publication No. 1997-64430 to process the dried grains of soybeans step by step It has been disclosed in the manufacturing method of the cheonggukjang to remove the disgusting smell. However, when manufacturing the cheonggukjang by the method described above is effective in reducing the discomfort of the cheonggukjang to some extent, due to the use of heterogeneous raw materials that inhibit the taste of the cheonggukjang, the taste of the traditional Cheonggukjang is not expressed, it makes you feel heterogeneous when eating and drinking As a result, the disadvantage that the degree of preference is rather reduced and the manufacturing method has an excessively complicated disadvantage.
한편, 맥반석은 진흙과 갯벌 해저층의 해초, 어패류, 용암분 등 바다의 모든 물질이 수억년간 퇴적되어 응회암으로 형성된 바위덩어리로 각종 미네랄 등 45종 이상의 원소 집합체이며, 인체에 해로운 물질을 흡착 분해하며 냄새를 없애는 탈취효과가 뛰어난 광물이다. 또한, 맥반석은 물을 뿌려주면 강한 생명력을 지닌 황토냄새가 나는 특성이 있고, 상온 또는 고온에서는 원적외선을 방출하여 건강증진에 효과가 있는 것으로 알려져 있다. 식수에 첨가하면 놀랄만한 정수작용을 하며, 특히 어깨결림, 신경통, 요통, 만성피로회복, 혈액순환 등에 효과가 있고, 신진대사를 촉진시켜 기의 흐름을 원활하게 하는 것으로 알려져 있다. 따라서, 사람의 체온인 36 내지 37℃까지 열을 올리면 원적외선이 방출되고, 부드러운 느낌을 주며 피부미용, 노화방지, 스트레스해소, 관절염 등의 치료에 효과를 나타내는 것으로 알려져 있다. 특히 동의보감에 의하면 맥반석이 피부병, 부스럼, 여드름 등의 상처 치유와 진통에 효능이 있는 것으로 기재되어 있다. 그러나, 이러한 맥반석을 청국장의 불쾌취를 제거하기 위한 용도로 사용된 예는 거의 전무한 상태이다.Elvan is a rock mass formed of tuff, which has been deposited with hundreds of millions of years of mud and tidal-flat seaweeds, fish and shellfish, and lava powder. It is an aggregate of more than 45 elements including various minerals. It is a mineral with excellent deodorizing effect that eliminates odor. In addition, it is known that elvan has the characteristic of ocher with strong vitality when sprayed with water, and emits far infrared rays at room temperature or high temperature, and is effective for health promotion. When added to drinking water it is a remarkable water purification effect, especially stiff shoulders, neuralgia, low back pain, chronic fatigue recovery, blood circulation, etc., and is known to promote the metabolism to smooth the flow of chi. Therefore, it is known that heating up to 36 to 37 ° C., which is a human body temperature, causes far-infrared rays to be released, gives a soft feeling, and has an effect on skin beauty, anti-aging, stress relief, and arthritis. In particular, agrees that elvan has been shown to be effective in healing and pain in wounds such as skin diseases, swelling and acne. However, there are almost no examples of the use of the ganbanseok for the purpose of removing the odor of Cheonggukjang.
따라서, 본 발명자들은 청국장 제조시 발효균주인 바실러스 서브틸리스 첨가와 동시에 맥반석을 첨가하여 발효시, 불쾌취가 제거된 청국장 추출물이 제조됨을 확인함으로써 본 발명을 완성하였다.Therefore, the present inventors have completed the present invention by confirming that the fermentation, the addition of the Bacillus subtilis fermentation strains in the production of the Cheonggukjang and the addition of the bancheukjang during the fermentation, the production of the Cheonggukjang extract is removed.
본 발명은 맥반석을 이용하여 불쾌취를 제거시킨 청국장 추출물의 제조방법을 제공하는 것을 목적으로 한다. It is an object of the present invention to provide a method for preparing cheonggukjang extract from which an unpleasant odor is removed by using elvan.
또한, 본 발명은 상기 제조방법에 의해 수득된 청국장 추출물을 포함한 건강기능식품을 제공하는 것을 목적으로 한다.
In addition, an object of the present invention is to provide a health functional food containing the extract of Cheonggukjang obtained by the manufacturing method.
상기의 목적을 달성하기 위하여, 본 발명은 (1) 대두를 물에 수침시키고, 세절한 다음 증자대두를 수득하는 제 1단계, (2) 상기 제 1단계에서 수득된 증자대두를 건조한 후, 바실러스 서브틸리스(Bacillus subtilis)를 물에 현탁시켜 미생물 포자현탁액을 수득하여 상기 건조된 증자대두에 0.5 내지 2 중량% 접종하는 제 2단계, (3) 적당한 크기로 절단한 맥반석을 상기 제 2단계의 접종된 증자대두에 설치하여 발효시켜 발효물을 수득하는 제 3단계, 및 (4) 상기 제 3단계에서 수득한 발효물을 분무건조 또는 열풍건조하여 분말화하는 제 4단계를 포함하는 제조공정으로 이루어진 불쾌취가 제거된 청국장 추출물의 제조방법을 제공한다.In order to achieve the above object, the present invention (1) the first step of immersing soybeans in water, and then chopped soybean soybean obtained, (2) after drying the steamed soybean obtained in the first step, Bacillus A second step of suspending Bacillus subtilis in water to obtain a microbial spore suspension and inoculating 0.5 to 2% by weight of the dried steamed soybean, and A third step of obtaining fermented product by fermentation by installing in inoculated cooked soybean and (4) a fourth step of powdering the fermented product obtained in the third step by spray drying or hot air drying. It provides a method for producing the cheongukjang extract from which the odor is made.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명의 불쾌취가 제거된 청국장 추출물을 제조하는 제 1단계는 대두를 물에 수침시키고, 세절한 다음 증자대두를 수득하는 단계이다. 이때, 대두는 청국장 제조를 위해 사용할 수 있는 통상의 콩류를 사용할 수 있으나, 강원도산 중태콩인 것이 바람직하다. 껍질을 제거시키거나 원형 그대로 2 내지 4배의 물에 8 내지 24시간동안 수침시켜 불린다. 다음으로, 물을 제거하고, 대두의 크기가 큰 경우에는 적절한 크기로 세절하는 바, 직경 0.5 내지 1.5㎝ 정도가 되도록 세절하는 것이 바람직하다. 세절이 끝난 다음에는 통상적인 찜통이나 압력증자기 등을 이용하여 대두를 90 내지 130℃의 온도에서 30 내지 60분동안 증자시키는 바, 증자에 의해서 노란색의 단단한 대두가 갈색으로 무른 증자대두가 수득되며, 제 2단계 공정을 수행하기 전 증자대두를 충분히 방냉시킨다.The first step of preparing the cheongukjang extract from which the unpleasant odor of the present invention is removed is to soak the soybeans in water, chop it, and then obtain steamed soybean. In this case, the soybean may be used a normal soybeans that can be used for the production of Cheonggukjang, it is preferred that it is Jungtae beans from Gangwon-do. It is called by removing the shell or by soaking in 2-4 times of water for 8 to 24 hours as it is. Next, when the water is removed and the size of the soybean is large, it is preferable to cut it to an appropriate size, so that the diameter is about 0.5 to 1.5 cm. After cutting, the soybeans are cooked for 30 to 60 minutes at a temperature of 90 to 130 ° C. using a conventional steamer or a pressure steamer. Allow the cooked soybeans to sufficiently cool before performing the second stage process.
본 발명의 제 2단계는 상기 제 1단계에서 수득된 증자대두를 건조한 후, 바실러스 서브틸리스(Bacillus subtilis)를 물에 현탁시켜 미생물 포자현탁액을 수득하여 상기 건조된 증자대두에 0.5 내지 2 중량% 접종하는 단계이다. 구체적으로, 바실러스 서브틸리스(Bacillus subtilis)를 물에 현탁시켜 미생물 포자 106 내지 107개/㎖이 되도록 미생물 포자현탁액을 수득한 다음, 상기 제 1단계에서 수득하여 방냉시킨 증자대두에 중량비로 0.5 내지 2 중량% 접종하고, 증자대두와 미생물 포자현탁액을 잘 혼합해 준다.In the second step of the present invention, after drying the cooked soybean obtained in the first step, Bacillus subtilis is suspended in water to obtain a microbial spore suspension, and 0.5 to 2% by weight of the dried soybean. Inoculation step. Specifically, Bacillus subtilis ( Bacillus subtilis ) is suspended in water to obtain a microbial spore suspension so that the microbial spores 10 6 to 10 7 / ml, and then obtained in the first step by weight ratio to the steamed soybean soybean Inoculate 0.5 to 2% by weight, and mix the soybean soybean and microorganism spore suspension well.
본 발명의 제 3단계는 적당한 크기로 절단한 맥반석을 상기 제 2단계의 접종된 증자대두에 설치하여 발효시키는 단계이다. 이때, 맥반석은 남해안 일대에 분포된 맥반석으로 2㎝ × 5㎝ × 10㎝ 의 크기로 세절한 다음, 25 내지 30 ㎠ 내의 범위에 한 절편씩 설치한다. 이후, 30 내지 35℃의 온도에서 12 내지 24시간동안 1차 발효, 35 내지 40℃의 온도에서 12 내지 24시간동안 2차발효, 마지막으로 39 내지 40.5℃의 온도에서 12 내지 24시간동안 3차발효시킨 후, 상온에서 3 내지 5시간 방치한 다음 발효물을 수득한다. 이때, 발효를 위한 환경으로서 황토방에서 실시하는 것이 바람직하다.The third step of the present invention is a step of fermenting by installing the ganban stone cut to a suitable size in the inoculated cooked soybean of the second step. At this time, the elvan is divided into elvan with 2 cm x 5 cm x 10 cm, which is distributed in the southern coastal area, and then one piece is installed in the range of 25 to 30 cm 2. Then, primary fermentation for 12 to 24 hours at a temperature of 30 to 35 ℃, secondary fermentation for 12 to 24 hours at a temperature of 35 to 40 ℃, finally tertiary for 12 to 24 hours at a temperature of 39 to 40.5 ℃ After fermentation, the mixture is left at room temperature for 3 to 5 hours and then fermented product is obtained. At this time, it is preferable to carry out in ocher as environment for fermentation.
부가적으로, 제 4단계에서는 상기 제 3단계에서 수득한 발효물을 40℃ 이하로 냉각시킨 후, 75℃ 이하에서 분무건조하여 수분함량 8% 이하로 건조한 후, 80 메쉬 이상으로 분말화하여 청국장 분말을 수득한다.Additionally, in the fourth step, the fermented product obtained in the third step is cooled to 40 ° C. or less, spray-dried at 75 ° C. or less, dried to 8% or less of water content, and then powdered to 80 mesh or more in Cheonggukjang. Obtain a powder.
상기 공정에 의해 생산된 청국장 분말을 이용하여 제조한 청국장은 다량의 점질물을 분비하고, 맛과 향이 우수할 뿐만 아니라 발효시켜 얻어진 청국장에서 불쾌취가 발생하지 않는다.Cheonggukjang prepared using the Cheonggukjang powder produced by the above process secretes a large amount of viscous substances, has excellent taste and aroma, and does not cause unpleasant odor in the Cheonggukjang obtained by fermentation.
또한, 본 발명은 상기 방법에 의해 생산된 청국장 추출물을 함유하고 식품학적으로 허용가능한 식품보조 첨가제를 포함하는 건강기능식품을 제공한다.In addition, the present invention provides a health functional food containing the Cheonggukjang extract produced by the method and comprising a food supplement acceptable food supplement.
본 발명의 기능성 식품 조성물은 건강 기능 식품, 건강 음료 또는 음료첨가제로 사용할 수 있으며, 상기 기능성 식품 조성물은 식품 총 중량에 대하여 0.01 내지 80 중량%로 혼합하여 기능성 음료, 환제, 정제, 이유식 또는 상기 성분을 건조 분말화하여 충진한 연질 또는 경질 캡슐제의 형태로 이용하는 것이 바람직하다.Functional food composition of the present invention can be used as a health functional food, health beverage or beverage additives, the functional food composition is mixed with 0.01 to 80% by weight based on the total weight of the food functional drinks, pills, tablets, baby food or the ingredients It is preferable to use in the form of soft or hard capsules filled with dry powder.
또한, 본 발명의 추출물은 식품 및 음료 등에 다양하게 이용될 수 있다. 본 청국장 추출물을 첨가할 수 있는 식품으로는, 예를 들어, 각종 식품류, 캔디, 초콜릿, 음료, 껌, 차, 비타민 복합제, 건강보조 식품류 등이 있고, 분말, 과립, 정제, 환제, 캡슐, 이유식 또는 음료인 형태로 사용할 수 있다.In addition, the extract of the present invention can be used in a variety of food and beverages. Examples of the food to which the Cheonggukjang extract can be added include various foods, candy, chocolate, beverages, gums, teas, vitamin complexes, health supplements, and the like, powders, granules, tablets, pills, capsules, and baby foods. Or in the form of a beverage.
본 발명의 건강 음료 조성물은 지시된 비율로 필수 성분으로서 상기 조성물을 함유하는 외에는 액체성분에는 특별한 제한점은 없으며 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상술한 천연 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등의 디사카라이드, 예를 들어 말토스, 슈크로스 등의 폴리사카라이드, 예를 들어 덱스트린, 시클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 상술한 것 이외의 향미제로서 천연 향미제(타우마틴, 스테비아 추출물(예를 들어 레바우디오시드 A, 글리시르히진등) 및 합성 향미제(사카린, 아스파르탐 등)를 유리하게 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 조성물 100 ㎖당 일반적으로 약 1 내지 20g, 바람직하게는 약 5 내지 12g이다.The health beverage composition of the present invention has no particular limitation on the liquid component except for containing the composition as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, like ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose, for example polysaccharides such as maltose and sucrose, and conventional sugars such as dextrin and cyclodextrin. And sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.
상기 외에 본 발명의 조성물은 여러가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있다. 그밖에 본 발명의 조성물들은 천연 과일 쥬스 및 과일 쥬스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다. In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof. , Organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination.
본 발명의 추출물은 독성 및 부작용이 거의 없으므로 예방 목적으로 장기간 복용시에도 안심하고 사용할 수 있다.Since the extract of the present invention has little toxicity and side effects, it can be used safely even when taken for a long time for the purpose of prevention.
본 발명의 조성물은 식품 또는 음료에 첨가될 수 있다. 이때, 식품 또는 음료 중에 본 발명의 상기 식품 조성물의 양은 일반적으로 전체 식품 중량의 20 내지 70 중량%로 가할 수 있으며, 음료 조성물에는 100 ㎖를 기준으로 0.02 내지 30 g, 바람직하게는 0.3 내지 1 g의 비율로 본 발명의 식품조성물을 가할 수 있다. The compositions of the present invention can be added to food or beverages. At this time, the amount of the food composition of the present invention in the food or beverage is generally of the total food weight 20 to 70% by weight, and the beverage composition may be added to the food composition of the present invention in a ratio of 0.02 to 30 g, preferably 0.3 to 1 g based on 100 ml.
또한, 본 발명은 상기 청국장 추출물을 유효성분으로 함유하는 식품 조성물이 제과·제빵, 면 종류, 두부, 육가공류, 김치를 포함한 발효식품 등의 식품가공 생산공정 과정이나 완제품 상에서 5 내지 50,000 ppm의 농도로 첨가되어, 항산화제의 작용으로 보존기간 및 유통기간이 연장되는 효과가 있는 천연보존료 기능을 갖는 식품첨가물을 제공한다.In addition, the present invention is a concentration of 5 to 50,000 ppm in the food processing production process or finished products, such as fermented foods, such as confectionery, bakery, cotton type, tofu, meat processing, kimchi is a food composition containing the Cheonggukjang extract as an active ingredient It is added to, to provide a food additive having a natural preservative function has the effect of extending the shelf life and shelf life by the action of antioxidants.
또한, 본 발명은 식품첨가제를 식품에 살균제, 향신료, 조미제, 여러가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산 음료에 사용되는 탄산화제 등 또는 식품소재의 필수원료로 사용하는 것을 특징으로 하는 식품첨가제의 이용방법을 제공한다. In addition, the present invention is a food additive additive in food, such as fungicides, spices, seasonings, various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, such as colorants and neutralizing agents (cheese, chocolate, etc.), Pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, etc. It provides a method of using a food additive.
이 때 식품첨가제는 식품에 침지, 분무 또는 혼합하여 상기 식품에 첨가할 수 있으며, 이러한 첨가제의 비율은 그렇게 중요하진 않지만 본 발명의 조성물 100 중량부 당 0.01 내지 80 중량부의 범위에서 선택되는 것이 일반적이다.At this time, the food additive may be added to the food by immersing, spraying or mixing the food, and the ratio of such additive is not so important, but per 100 parts by weight of the composition of the present invention It is generally selected in the range of 0.01 to 80 parts by weight.
상기 식품은 과일, 야채, 과일이나 야채의 건조제품이나 절단제품, 과일쥬스, 야채쥬스, 이들의 혼합쥬스이거나 산성음료수를 포함하는 음료류, 쿠키, 사탕, 카라멜, 껌 등과 같은 제과류, 제빵류, 아이스크림 제품류, 다(茶)류, 요구르트와 같은 발효유식품, 유가공식품, 양념류, 주류, 통조림 또는 병조림류, 면류, 축산가공식품, 수산가공식품, 미생물발효식품, 두류식품, 곡류식품, 육가공류, 감초류, 허브류 중 어느 하나 또는 하나 이상이다. The foods are fruits, vegetables, dried or cut products of fruits or vegetables, fruit juices, vegetable juices, mixed juices or beverages containing acidic beverages, cookies, candies, caramels, gums, etc. Products, fermented milk products such as tea, yogurt, dairy foods, condiments, alcoholic beverages, canned or bottled foods, noodles, livestock processed foods, fish processed foods, microbial fermented foods, soybean foods, cereals, meat processed foods, licorice Or at least one of herbs and herbs.
본 발명은 다음의 실시예 및 실험예에 의거하여 더욱 상세히 설명되나, 본 발명이 실시예 또는 실험예에 의해 제한되지는 않는다.The present invention is described in more detail based on the following examples and experimental examples, but the present invention is not limited to the examples or experimental examples.
실시예 1. 청국장 추출물 분말의 제조Example 1 Preparation of Cheonggukjang Extract Powder
강원도산 중태콩 5 ㎏을 정선하여 깨끗이 세척한 다음, 껍질을 제거하여 3배의 물에 10 시간동안 수침시켜 불렸다. 그 후, 물기를 제거하고, 대두를 직경 0.5 내지 1.5㎝ 정도가 되도록 세절한 다음, 0.9 ㎏/㎠의 압력을 발생시키는 고압솥을 이용하여 121℃에서 40분동안 증자시켰다. 증자된 증자대두를 37℃ 온도에서 충분히 방냉시킨 다음, 바실러스 서브틸리스(Bacillus subtilis)(KCTC)를 1㎖ 당 106 내지 107개/㎖이 되도록 물에 현탁시키고, 미생물 포자현탁액을 발효물 중량의 1 중량% 정도가 되도록 접종하여 잘 혼합하였다. 그 후, 남해안 보성 또는 고흥 일대에 분포된 맥반석으로 2㎝ × 5㎝ × 10㎝ 의 크기로 세절한 다음, 25 내지 30 ㎠ 내의 범위에 한 절편씩 설치한다. 이후, 30℃의 온도에서 24시간, 40℃의 온도에서 24시간, 마지막으로 40.5℃의 온도에서 24시간동안 발효시킨 후, 상온에서 3 시간 방치한 다음 발효물(2.72 ㎏)을 수득하였다. 이후, 발효물을 30 ~ 40℃로 냉각시킨 후, 60 ~ 75℃에서 분무건조하여 수분함량 8% 이하로 건조한 후, 90 메쉬로 분말화하여 청국장 추출물 분말(850 g)을 수득하였다.5 kg of Taekwon beans in Gangwon-do were selected and washed thoroughly, and then peeled and soaked in three times of water for 10 hours. Thereafter, the water was removed, the soybeans were chopped to about 0.5 to 1.5 cm in diameter, and then steamed at 121 ° C. for 40 minutes using an autoclave generating a pressure of 0.9 kg / cm 2. The steamed cooked soybean is allowed to cool sufficiently at 37 ° C, and then Bacillus subtilis (KCTC) is suspended in water at 10 6 to 10 7 / ml per ml, and the microbial spore suspension is fermented. Inoculated to about 1% by weight of the weight and mixed well. After that, it was chopped into 2cm × 5cm × 10cm with elvan stones distributed in Boseong or Goheung area of the south coast, and then 25 to 30 Install one piece at a range within cm 2. Thereafter, the mixture was fermented at a temperature of 30 ° C. for 24 hours, at a temperature of 40 ° C. for 24 hours, and finally at 40.5 ° C. for 24 hours, and then left at room temperature for 3 hours to obtain a fermentation product (2.72 kg). Thereafter, the fermented product was cooled to 30 to 40 ° C., spray dried at 60 to 75 ° C., dried to 8% or less of moisture, and then powdered to 90 mesh to obtain Cheonggukjang extract powder (850 g).
비교예 1. 통상의 청국장 추출물 분말의 제조Comparative Example 1. Preparation of conventional Cheonggukjang extract powder
상기 실시예 1에서 제조된 불쾌취가 제거된 청국장과 특성, 기호도 및 활성 성분을 비교분석하기 위한 비교예로서의 청국장 추출물 분말을 제조하였다.Cheonggukjang extract powder was prepared as a comparative example to compare the characteristics, preference and active ingredient with the cheonggukjang removed from the odor is prepared in Example 1.
구체적으로, 강원도산 중태콩 5 ㎏을 정선하여 깨끗이 세척한 다음, 껍질을 제거하여 3배의 물에 10 시간동안 수침시켜 불렸다. 그 후, 물기를 제거하고, 대두를 직경 0.5 내지 1.5㎝ 정도가 되도록 세절한 다음, 0.9 ㎏/㎠의 압력을 발생시키는 고압솥을 이용하여 121℃에서 40분동안 증자시켰다. 증자된 증자대두를 37℃ 온도에서 충분히 방냉시킨 다음, 바실러스 서브틸리스(Bacillus subtilis)(KCTC)를 1㎖ 당 106 내지 107개/㎖이 되도록 물에 현탁시키고, 미생물 포자현탁액을 발효물 중량의 1 중량% 정도가 되도록 접종하여 잘 혼합하였다. 그 후, 38℃의 온도에서 48시간동안 발효시킨 후, 상온에서 3 시간 방치한 다음 발효물(2.59 ㎏)을 수득하였다. 이후, 발효물을 40℃ 이하로 냉각시킨 후, 75℃ 이하에서 분무건조하여 수분함량 8% 이하로 건조한 후, 80 메쉬 이상으로 분말화하여 청국장 추출물 분말(837 g)을 수득하였다.Specifically, 5 kg of Taekwon beans in Gangwon-do were selected and washed, and then the skin was removed and soaked in three times of water for 10 hours. Thereafter, the water was removed, the soybeans were chopped to about 0.5 to 1.5 cm in diameter, and then steamed at 121 ° C. for 40 minutes using an autoclave generating a pressure of 0.9 kg / cm 2. The steamed cooked soybean is allowed to cool sufficiently at 37 ° C, and then Bacillus subtilis (KCTC) is suspended in water at 10 6 to 10 7 / ml per ml, and the microbial spore suspension is fermented. Inoculated to about 1% by weight of the weight and mixed well. Thereafter, the mixture was fermented at a temperature of 38 ° C. for 48 hours, and then left at room temperature for 3 hours to obtain fermented product (2.59 kg). Thereafter, the fermented product was cooled to 40 ° C. or less, spray dried at 75 ° C. or less, dried to 8% or less of water content, and then powdered to 80 mesh or more to obtain Cheonggukjang extract powder (837 g).
실험예 1. 청국장의 이화학 및 관능적 특성 조사Experimental Example 1. Investigation of Physicochemical and Sensory Characteristics of Cheonggukjang
실시예 1 및 비교예 1에서 수득된 청국장에 대한 pH, 적정산도 및 색도의 이화학적 특성과 관능적 특성을 조사 및 평가하였다.The physicochemical and organoleptic properties of pH, titratable acidity and chromaticity of Cheonggukjang obtained in Example 1 and Comparative Example 1 were investigated and evaluated.
pH는 청국장 추출물 10g에 증류수 40㎖을 가하여 희석시킨 다음 pH 미터기로 측정하였으며, 적정산도는 청국장 10g에 증류수 40㎖을 가하여 잘 혼합한 다음 0.1N 농도의 수산화나트륨 용액으로 pH 8.3이 될 때까지 적정하고, 소비된 0.1N 농도의 수산화나트륨 용액의 ㎖수로 표시하였다. 또한, 관능적 특성은 잘 훈련된 10명의 검사요원을 선발하여, 불쾌한 냄새, 구수한 맛, 전체적인 기호도인 총평 등을 9점 채점법(아주좋음(9), 보통(6), 나쁨(3))으로 평가하게 하였다(표 1 참조).The pH was diluted by adding 40 ml of distilled water to 10 g of Cheonggukjang extract and measured with a pH meter. The titratable acidity was mixed well by adding 40 ml of distilled water to 10 g of Cheonggukjang and then titrated until the pH was 8.3 with a 0.1 N sodium hydroxide solution. And the number of ml of sodium hydroxide solution consumed at 0.1 N concentration. In addition, the sensory characteristics were selected by 10 well-trained inspectors, and evaluated the unpleasant odor, the tasteful taste, and the overall rating of overall preference by 9 point scoring method (very good (9), moderate (6), bad (3)). (See Table 1).
그 결과, 상기 표 1에서 보는 바와 같이 실시예 1의 불쾌취가 제거된 청국장 추출물이 통상적인 발효공정에 의한 비교예 1에서 수득된 청국장 추출물에 비하여 pH 및 적정산도의 유지가 가능했으며, 관능검사 결과에 있어서도 탁월한 불쾌취 제거효과는 보였으며, 구수한 맛도 강해져서 전체적인 기호도가 높음을 확인하였다.As a result, as shown in Table 1, the odor of Cheonggukjang extract of Example 1 was removed, compared to the Cheonggukjang extract obtained in Comparative Example 1 by the conventional fermentation process was able to maintain the pH and titratable acidity, sensory test In the results, it showed an excellent odor removal effect, and the taste was also strong, confirming that the overall preference was high.
실험예 2. 청국장 추출물의 질소화합물 분석Experimental Example 2. Analysis of Nitrogen Compounds in Extracts of Cheonggukjang
상기 실시예 1 및 비교예 1에서 제조된 청국장 추출물의 질소화합물을 분석하기 위하여, 조단백질 함량은 마이크로 켈달(micro-Kjeldahl) 방법(A.O.A.C., Official Methods of analysis, 11th ed., Association of Official Analytical Chemists. Washington. D.C., 1980), 아미노태 질소량은 포몰(formol)적정법(김재욱, 식품학 실험서, 개문사, 1985)에 따라 시료를 전처리한 후 분석하였다.In order to analyze the nitrogen compounds of the Chunggukjang extracts prepared in Example 1 and Comparative Example 1, crude protein content was determined by the micro-Kjeldahl method (AOAC, Official Methods of analysis, 11th ed., Association of Official Analytical Chemists. Washington, DC, 1980), and amino nitrogen levels were analyzed after pretreatment of the samples according to the formol titration method (Jae-Wook Kim, Food Science Experiment, Gaemunsa, 1985).
그 결과, 실시예 1의 청국장 추출물의 조단백질 함량은 62.1%로서 원료콩의 조단백질 함량 39.02%와 비교예 1의 청국장 조성물의 조단백질 함량 50.9%보다 우수하였으며, 불쾌취의 원인물질인 아미노태 질소량이 현저히 적었다(표 2 참조).As a result, the crude protein content of the Cheonggukjang extract of Example 1 was 62.1%, which was superior to the crude protein content of 39.02% of the raw soybeans and 50.9% of the crude protein content of the Cheonggukjang composition of Comparative Example 1, and the amount of amino nitrogen, which is a source of unpleasant odor, was remarkable. (See Table 2).
실험예 3. 청국장 추출물의 성분분석Experimental Example 3. Component Analysis of Cheonggukjang Extract
상기 실시예 1 및 비교예 1에서 수득된 청국장 추출물에 대한 구체적인 성분분석을 유리당, 유기산, 지방산 및 아미노산 항목으로 나누어 조사하였다.Specific component analysis of the extracts of Cheonggukjang obtained in Example 1 and Comparative Example 1 were investigated by dividing into free sugar, organic acid, fatty acid and amino acid.
3-1. 청국장 추출물의 유리당 함량 분석3-1. Free Sugar Content Analysis of Cheonggukjang Extract
상기 실시예 1 및 비교예 1에서 수득한 청국장 추출물 4g을 에탄올 50㎖에 현탁시키고, 실온에서 5시간동안 진탕교반한 다음, 4℃의 온도에서 하룻밤 방치하였으며, 이를 4,000rpm에서 30분간 원심분리한 후 상등액만을 분리하였다. 전기 상등액을 셉펙 C18 카트리지(Seppak C18 cartridge, Waters, U.S.A.)에 통과시키고, 다시 0.45㎛ 멤브레인 필터로 여과한 후, HPLC(Waters, U.S.A.)로 다양한 유리당의 함량을 각 유리당 표준액을 기준으로 분석하였다(표 3 참조).4 g of the Cheonggukjang extracts obtained in Examples 1 and Comparative Example 1 were suspended in 50 ml of ethanol, shaken at room temperature for 5 hours, and left overnight at a temperature of 4 ° C., which was centrifuged at 4,000 rpm for 30 minutes. Only the supernatant was separated. The electric supernatant was passed through a Seppak C18 cartridge (Waters, USA), again filtered through a 0.45 μm membrane filter, and then analyzed by HPLC (Waters, USA) to determine the content of various free sugars based on the standard of each glass. See Table 3).
상기 표 3에서 알 수 있는 바와 같이, 각 청국장 추출물의 유리당 함량은 비슷한 것으로 나타나, 청국장에 포함되는 당성분의 특성에는 큰 상이점이 없는 것으로 나타났다.As can be seen in Table 3, the free sugar content of each of the cheonggukjang extract was shown to be similar, there was no significant difference in the properties of the sugar component contained in the cheonggukjang.
3-2. 청국장 추출물의 유기산 함량 분석3-2. Analysis of Organic Acid Content in Cheonggukjang Extracts
상기 실시예 1 및 비교예 1에서 수득한 청국장 추출물 1g을 50㎖의 0.1N-HCl 용액에 첨가하여 현탁시키고, 2시간동안 진탕교반한 다음, 20% 설포살린산(sulfosalicylic acid)을 50㎖ 첨가하고, 3시간동안 진탕추출하여 청국장 내에 포함된 단백질을 제거시켰다. 다음으로, 4,000rpm에서 30분간 현탁액을 원심분리하여 상등액을 수득하였으며, 이를 셉펙 C18 카트리지에 통과시키고, 다시 0.45㎛ 멤브레인 필터로 여과한 후, HPLC로 다양한 유리산의 함량을 각 유기산 표준액을 기준으로 하여 분석하였다(표 4 참조).1 g of Cheonggukjang extracts obtained in Example 1 and Comparative Example 1 was added to 50 ml of 0.1N-HCl solution, suspended and stirred for 2 hours, and then 50 ml of 20% sulfosalicylic acid was added. And shaken for 3 hours to remove the protein contained in Cheonggukjang. Next, the supernatant was obtained by centrifuging the suspension at 4,000 rpm for 30 minutes, which was passed through a Sepp C18 cartridge, again filtered through a 0.45 μm membrane filter, and then the contents of various free acids were determined by HPLC based on each organic acid standard. And analyzed (see Table 4).
상기 표 4에서 알 수 있는 바와 같이, 실시예 1에서 제조된 본 발명의 불쾌취가 제거된 청국장의 경우가 비교예에서 제조된 청국장에 비하여 유기산 함량이 전체적으로 많다는 것을 확인 할 수 있었다.As can be seen in Table 4, it can be confirmed that the organic acid content of the chungkukjang prepared in Example 1 is removed from the odor of the present invention is removed in Comparative Example 1 as a whole.
3-3. 청국장 추출물의 지방산 함량 분석3-3. Fatty Acid Content Analysis of Cheonggukjang Extract
상기 실시예 1 및 비교예 1에서 수득한 청국장 추출물 0.2g을 추출용매인 에탄올 5㎖와 내부표준물질인 0.5㎎ PDA(pentadecanoic acid) 1㎖에 용해시키고, 10분동안 소니케이터(Brauson, Germany)로 활성화하였으며, NaCl 4㎖을 첨가하여 5분동안 2,000rpm으로 원심분리하여 상등액을 제거하고, 나머지 부분을 질소가스를 이용하여 건조시켰다. 다음으로, 건조물에 톨루엔 0.5㎖ 및 NaOH 2㎖을 첨가하고 40℃의 온탕에서 3분동안 반응시켰으며, 다시 톨루엔 0.5㎖ 및 NaOH 2㎖을 첨가하여 40℃의 온탕에서 3분동안 재반응시키고, 질소가스를 이용하여 방냉시킨 다음, 보론 트리플로라이드(BF3) 용액 2㎖을 첨가하여 5분동안 반응시켰다. 전기 반응물을 증류수와 PE(petroleum ether)를 첨가하여 분리시킨 다음, 상등액의 수분을 피펫으로 제거시키고, 가스 크로마토그래피(gas chromatography, Hewlett-Packard 5890 series Ⅱ, U.S.A.)로 다양한 유리산의 함량을 분석하였다(표 5 참조).0.2 g of Cheonggukjang extracts obtained in Example 1 and Comparative Example 1 were dissolved in 5 ml of ethanol as an extraction solvent and 1 ml of 0.5 mg PDA (pentadecanoic acid) as an internal standard, and then used for 10 minutes in a sonicator (Brauson, Germany). The supernatant was removed by centrifugation at 2,000 rpm for 5 minutes by adding 4 ml of NaCl, and the rest was dried using nitrogen gas. Next, 0.5 ml of toluene and 2 ml of NaOH were added to the dried product and reacted for 3 minutes in a hot water bath of 40 ° C. Then, 0.5 ml of toluene and 2 ml of NaOH were added for re-reaction for 3 minutes in a hot water bath of 40 ° C. After cooling with nitrogen gas, 2 ml of boron trifluoride (BF 3 ) solution was added and reacted for 5 minutes. The reactants were separated by the addition of distilled water and petroleum ether (PE), followed by pipetting to remove water from the supernatant and analyzing the contents of various free acids by gas chromatography (Hewlett-Packard 5890 series II, USA). (See Table 5).
상기 표 5에서 알 수 있는 바와 같이, 실시예 1에서 제조된 본 발명의 불쾌취가 제거된 청국장의 경우가, 비교예 1에서 제조된 청국장에 비하여 불포화지방산인 리놀레산과 리노렌산 함량이 높게 나타남과 동시에, 전체적으로 높은 지방산 함량을 내포한다는 것을 확인할 수 있었다.As can be seen in Table 5, in the case of Cheonggukjang without the odor of the present invention prepared in Example 1, the content of linoleic acid and linolenic acid which is unsaturated fatty acid is higher than that of Cheonggukjang prepared in Comparative Example 1 and At the same time, it was confirmed that it contained a high fatty acid content as a whole.
3-4. 청국장 추출물의 아미노산 함량 분석3-4. Amino Acid Content Analysis of Cheonggukjang Extract
상기 실시예 1 및 비교예 1에서 수득한 청국장 조성물 100g을 HPLC(Model LC 500, Biotronic, West Germany를 이용하여 데이비스 및 토마스(Davies, M.G. and Thomas, A.J., J. Sci. Food Agric., 24, p1525, 1973)와 메이슨 등(Mason, V.C., Bech-Anderson and Rudemo, M., Z. Tierernahrg. U. Futtermitte-lkde., 41, p226, 1979)의 방법에 따라 시료를 전처리 한 후 아미노산 함량을 분석하였다(표 6 참조).100 g of the Cheonggukjang composition obtained in Example 1 and Comparative Example 1 was prepared using HPLC (Model LC 500, Biotronic, West Germany) by Davis and Thomas (Davies, MG and Thomas, AJ, J. Sci. Food Agric. , 24, p1525, 1973) and amino acid content after pretreatment according to the method of Mason et al. (Mason, VC, Bech-Anderson and Rudemo, M., Z. Tierernahrg.U. Futtermitte-lkde. , 41, p226, 1979). Analyzes (see Table 6).
상기 표 6에서 알 수 있는 바와 같이, 원료콩 및 통상의 청국장 추출물의 아미노산 조성과 비교하여 볼때, 전반적으로 본 발명의 청국장 추출물이 아미노산 함량이 높았으며, 특히 글루탐산 함량이 특징적으로 높았다. As can be seen in Table 6, when compared with the amino acid composition of the raw soybeans and the conventional chungkukjang extract, the chungkukjang extract of the present invention had a high amino acid content, particularly glutamic acid content.
본 발명의 조성물은 아래와 같은 제형으로 투여할 수 있으며, 아래의 제제 실시예는 본 발명을 예시하는 것일 뿐, 이에 의해 본 발명의 내용이 제한되는 것은 아니다.The composition of the present invention can be administered in the following formulations, the formulation examples below are merely to illustrate the invention, whereby the content of the invention is not limited.
제제예 1. 건강기능식품의 제조Formulation Example 1 Preparation of Health Functional Food
실시예 1 추출물 1000 ㎎Example 1 1000 mg extract
비타민 혼합물 20 g20 g of vitamin mixture
비타민 A 아세테이트 70 ㎍70 μg of Vitamin A Acetate
비타민 E 1 ㎎Vitamin E 1 mg
비타민 B1 0.13 ㎎Vitamin B 1 0.13 mg
비타민 B2 0.15 ㎎Vitamin B 2 0.15 mg
비타민 B6 0.5 ㎎Vitamin B 6 0.5 mg
비타민 B12 0.2 ㎍0.2 μg of vitamin B 12
비타민 C 10 ㎎Vitamin C 10 mg
비오틴 10 ㎍10 μg biotin
니코틴산아미드 1.7 ㎎Nicotinic Acid 1.7 mg
엽산 50 ㎍Folate 50 ㎍
판토텐산 칼슘 0.5 ㎎Calcium Pantothenate 0.5mg
무기질 혼합물 적량Mineral mixture
황산제1철 1.75 ㎎Ferrous Sulfate 1.75 mg
산화아연 0.82㎎Zinc Oxide 0.82mg
탄산마그네슘 25.3 ㎎Magnesium carbonate 25.3 mg
제1인산칼륨 15 ㎎Potassium monophosphate 15 mg
제2인산칼슘 55 ㎎Dibasic calcium phosphate 55 mg
구연산칼륨 90 ㎎Potassium Citrate 90 mg
탄산칼슘 100 ㎎Calcium Carbonate 100 mg
염화마그네슘 24.8 ㎎Magnesium chloride 24.8 mg
상기 비타민 및 미네랄 혼합물의 조성비는 비교적 건강식품에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 그 배합비를 임의로 변형 실시하여도 무방하며, 통상의 건강기능식품 제조 방법에 따라 상기의 성분을 혼합한 다음, 과립을 제조하고, 통상의 방법에 따라 건강기능식품 조성물 제조에 사용할 수 있다.Although the composition ratio of the vitamin and mineral mixture is a composition suitable for a relatively healthy food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food production method. The granules may be prepared and used for preparing the nutraceutical composition according to a conventional method.
제제예 2. 건강기능음료의 제조Formulation Example 2 Preparation of Health Functional Drink
실시예 1 추출물 1000 ㎎Example 1 1000 mg extract
구연산 100 ㎎Citric acid 100 mg
올리고당 100 g100 g oligosaccharides
매실농축액 2 gPlum concentrate 2 g
타우린 1 g1 g of taurine
정제수를 가하여 전체 900㎖Add 900 ml of purified water
통상의 건강음료 제조방법에 따라 상기의 성분을 혼합한 다음, 약 1시간동안 85℃에서 교반 가열한 후, 만들어진 용액을 여과하여 멸균된 2ℓ 용기에 취득하여 밀봉 멸균한 뒤 냉장 보관한 다음 본 발명의 건강기능음료 조성물 제조에 사용한다.After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention It is used to prepare a health functional beverage composition.
제제예 3. 환제의 제조Formulation Example 3 Preparation of Pills
실시예 1 추출물 70 gExample 1 70 g of extract
산약 20 g20 g of powder
찹쌀전분 10 g10 g of glutinous rice starch
상기 실시예 1의 조성물, 산약, 찹쌀전분을 반죽한다. 혼합물을 살균 후 밀링기와 환주기를 통해 성형한 다음, 성형된 혼합물을 절환기를 통해 일정량 절단한다. 그 후, 일정량으로 절단된 혼합물을 정환기(당의기)로 성형한 다음, 성형된 환을 건조, 살균한 후 냉각하여 선별한다.Knead the composition, acid powder, glutinous rice starch of Example 1. The mixture is sterilized and then molded through a milling machine and a ring cycle, and then the molded mixture is cut in an amount through a changer. Thereafter, the mixture cut in a predetermined amount is molded into a ring machine (sugar dragee), and then the molded ring is dried, sterilized, cooled and sorted.
제제예 4. 이유식의 제조Formulation Example 4 Preparation of Baby Food
실시예 1의 추출물 1000㎎1000 mg of extract of Example 1
철분 0.1 ㎎0.1 mg of iron
비타민 A 80 ㎍80 μg of vitamin A
비타민 B1 0.13 ㎎Vitamin B 1 0.13 mg
비타민 B2 0.15 ㎎Vitamin B 2 0.15 mg
비타민 C 10 ㎎Vitamin C 10 mg
우유칼슘 100 ㎎Milk calcium 100 mg
통상의 이유식 제조방법에 따라 상기의 성분을 혼합한 다음, 약 1시간동안 30℃에서 정제수를 가하여 교반한 후, 100℃로 멸균한 다음 진공 건조기에서 약 760 ㎜Hg로 약 9시간 동안 건조한 다음 약 100 메쉬로 분쇄하여 포장한다.After mixing the above components according to a conventional baby food preparation method, the mixture was stirred by adding purified water at 30 ° C. for about 1 hour, sterilized at 100 ° C., and then dried at about 760 mmHg in a vacuum dryer for about 9 hours, and then Crushed to 100 mesh and packed.
상기 조성비는 비교적 기호음료에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만 수요계층이나, 수요국가, 사용용도 등 지역적, 민족적 기호도에 따라서 그 배합비를 임의로 변형 실시하여도 무방하다.Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.
이상과 같이, 본 발명에 의해 제조된 청국장 추출물은 전통적인 청국장의 맛이 재현됨과 동시에, 청국장 특유의 불쾌취가 효과적으로 감소되고 좋은 풍미를 발생시키면서 현대적으로 제조될 수 있으므로 본 발명의 제조방법에 의해 제조된 청국장은 전통적인 청국장의 맛이 재현됨과 동시에, 청국장 특유의 강한 불쾌취가 효과적으로 감소되고 풍부한 영양과 풍미를 나타내므로 영유아의 성장발달에 유용한 발효 이유식 및 건강보조식품에 이용될 수 있다.As described above, the cheonggukjang extract prepared by the present invention is produced by the production method of the present invention because the taste of the traditional Cheonggukjang can be reproduced at the same time, the discomfort peculiar to the Cheonggukjang can be effectively reduced and produce a good flavor. Cheonggukjang can be used in fermented baby foods and health supplements useful for the growth and development of infants, as the taste of traditional Cheonggukjang is reproducible, while the strong discomfort unique to Cheonggukjang is effectively reduced and rich nutrition and flavor are exhibited.
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