KR100581729B1 - A Contineous germination method of Korean Ginseng seed - Google Patents
A Contineous germination method of Korean Ginseng seed Download PDFInfo
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- KR100581729B1 KR100581729B1 KR1020020087430A KR20020087430A KR100581729B1 KR 100581729 B1 KR100581729 B1 KR 100581729B1 KR 1020020087430 A KR1020020087430 A KR 1020020087430A KR 20020087430 A KR20020087430 A KR 20020087430A KR 100581729 B1 KR100581729 B1 KR 100581729B1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/35—Bulbs; Alliums, e.g. onions or leeks
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Abstract
본 발명은 고려인삼종자의 연중발아법 및 그 발아식물에 관한 것으로 식물성장호르몬, 토양, 배지, 항진균 또는 항생제 처리없이 인삼종자를 연중 발아시킬 수 있는 방법을 제공하는 뛰어난 효과가 있다.The present invention relates to a year-round germination method of Korean ginseng seed and its germination plant has an excellent effect of providing a method for germinating ginseng seeds throughout the year without treatment of plant growth hormone, soil, medium, antifungal or antibiotics.
고려인삼종자, 발아, 식물성장호르몬, 배지, 항진균제, 항생제Korean ginseng seed, germination, plant growth hormone, medium, antifungal, antibiotic
Description
도 1은 무광(無光)하에 실시한 본 발명의 바람직한 실시예이다.1 is a preferred embodiment of the present invention carried out in the absence of light.
도 2는 유광(有光)하에 실시한 본 발명의 바람직한 실시예이다.2 is a preferred embodiment of the present invention carried out under a light.
본 발명은 고려인삼종자의 연중발아법 및 그 발아식물에 관한 것으로 더욱 상세하게는 온·습도 조절에 의한 인삼종자의 연중발아법 및 그 발아법에 의해 생산된 발아식물에 관한 것이다.The present invention relates to the annual germination method of Korean ginseng seed and its germination plant, and more particularly, to the germination plant produced by the germination method of the ginseng seed by temperature and humidity control and the germination method.
종래에는 인삼의 조직배양 기술에 의한 세포증식을 도모한 기술이 국내 특허 공고번호 91-4949 및 91-4950호가 공지되어 있으며 인삼종자로부터 직접 캘러스를 유도한 기술이 본 발명자들에 의해 세계최초로 공개한 기술이 국내특허 제307503호에 개시된 바 있다. 그러나 껍질이 존재하는 고려인삼종자로부터 개갑처리하지 않고 직접 발아를 유도한 기술은 지금까지 시도된 바 전무한 실정이며 특히 식품 또는 의약품용으로 사용하기에 적합하도록 식물생장호르몬, 항진균제 또는 항생제를 첨가하지 않고도 싱싱하고도 식품공업적으로 우수한 인삼종자 발아식물을 유도한 기술은 누구도 시도해 본 적이 없다.Conventionally, Korean Patent Publication Nos. 91-4949 and 91-4950 are known for cell proliferation by tissue culture technology of ginseng, and the technique which directly induces callus from ginseng seed was first disclosed by the present inventors in the world. The technology has been disclosed in Korean Patent No. 307503. However, techniques that induce germination directly from ginseng seed without shelling without any graft treatment have been attempted to date and have not been added to plant growth hormones, antifungal agents or antibiotics to make them suitable for use in food or medicine. No one has ever tried to induce fresh ginseng seed germination plants in the food industry.
본 발명은 상기와 같은 점들에 착안하여 안출한 것으로 인삼종자로부터 직접 발아를 유도함에 있어서 GA₃등 식물생장호르몬을 처리함이 없이 무토양, 무배지 상태에서 항진균제와 항생제를 처리함이 없이 껍질이 두꺼운 고려인삼종자로부터 직접 발아를 유도하여 그 발아식물을 제공하는데 그 목적이 있다.The present invention has been conceived in view of the above points in the induction of germination directly from ginseng seeds without the treatment of plant growth hormones such as GA₃ without thickening the shell without treating antifungal agents and antibiotics in a soil-free, medium-free state Its purpose is to induce germination directly from Korean ginseng seed and provide its germination plant.
본 발명의 상기 목적은 인삼종자에 저온과 고온조건 그리고 저습과 고습조건을 반복 처리한 후 발아상태를 관찰함으로써 달성하였다.
The object of the present invention was achieved by observing germination after repeated treatment of low and high temperature conditions and low and high humidity conditions for ginseng seeds.
본 발명은 종자를 저습상태에서 저온처리하는 단계와 다습상태에서 고온처리하는 단계를 1일 4∼6시간 간격으로 2∼4시간씩 3주 수행함을 특징으로 한다.The present invention is characterized in that the low temperature treatment of the seeds in a low humidity state and the high temperature treatment in a high humidity state are performed for 2 to 4 hours at intervals of 4 to 6 hours per day.
본 발명의 또 다른 특징은 본 발명자들이 연구하여 성공시킨 인삼종자의 캘러스(Callas) 와는 달리 MS 기초고체배지 또는 토양을 이용하지 아니할 뿐만 아니라 더욱이 일장처리 또는 GA₃등 식물생장조절제를 사용하는 일이 없이 인삼종자를 연중 계속적으로 발아시키고 그 발아식물을 제공하는데 그 특징이 있다.Another feature of the present invention is that unlike the callus (Callas) of the ginseng seed that the present inventors have studied and succeeded, not only does not use MS-based solid medium or soil, but also does not use a plant growth regulator such as sun treatment or GA₃. It is characterized by germinating ginseng seeds continuously throughout the year and providing germination plants.
더욱이, 본 발명은 발아과정이나 발아 후 식물성장에 영향을 미칠까 우려하 여 발아처리 전 또는 발아과정에 항진균제나 항생제를 처리하지 않는다는 데에도 그 특징이 있다.Furthermore, the present invention is characterized in that antifungal agents or antibiotics are not treated before or during germination because of fear of affecting plant growth after germination or after germination.
이와 같이 하여 발아처리한 인삼종자 발아유식물은 절삭 후 건조하거나 건조 후 절삭처리하여 분말화 함으로써 식품 또는 의약품의 원료로 사용하거나 직접 절삭후 분쇄하여 드링크 음료 또는 화장품 원료로 사용할 수 있는 잇점이 있다.The germinated seedlings of the ginseng seed germinated in this way can be used as raw materials for food or medicine by drying after cutting or cutting and drying after cutting, or can be used as raw materials for drinks or cosmetics by grinding after direct cutting.
인삼은 의약품으로서 전통적으로 애용되어 온 고가의 약용식물로서 사포닌, 알칼로이드, 페놀, 폴리아세틸렌 등 의약상 약효성분을 다량 함유하고 있다.Ginseng is an expensive medicinal plant traditionally used as a medicine and contains a large amount of pharmaceutical active ingredients such as saponin, alkaloids, phenol and polyacetylene.
이하, 본 발명의 구체적인 구성을 도면과 실시예를 들어 상세히 설명한다.Hereinafter, the specific configuration of the present invention will be described in detail with reference to the drawings and examples.
실시예 1 : 무광(無光)하의 인삼종자로부터 직접 발아 유도Example 1 Induction of Germination Directly from Ginseng Seeds Without Matte
고려인삼종자 10개를 샤레에 넣어 냉동고에 투입후 상대습도 60% 조건으로 -5℃에서 5시간 저온처리 시킨 다음, 다시 상대습도 90% 조건으로 20℃에서 온장고에 투입후 3시간 동안 고온 처리하였다.After putting 10 Korean Ginseng seeds into a curry, they were placed in a freezer for 5 hours at -5 ° C under a relative humidity of 60%, and then placed at 20 ° C under a relative humidity of 90% for 3 hours. .
그 후 또다시 -5℃에서 동일한 상대습도 조건에서 5시간 저온처리 시킨 다음 상대습도 90% 조건의 20℃에서 3시간 고온처리 하였다.Thereafter, the resultant was further cold-treated at -5 ° C for 5 hours at the same relative humidity condition, and then high-temperature-treated at 20 ° C at 90% relative humidity.
이와 같이 하루에 3회 반복 처리(reapeat treatment) 하기를 3주간 처리한 결과, 도 1에서 확인되는 바와 같이 엽아길이 최소 0.6㎝, 최대 2.5㎝의 고려인삼 종자 발아 식물을 얻었다.As described above, three times a day of repeat treatment (reapeat treatment) for three weeks, as shown in Figure 1, the ginseng seed germination plants of the minimum leaf length 0.6cm, 2.5cm maximum.
실시예 2 : 유광(有光)하에서의 발아 유도Example 2 Induction of Germination Under Gloss
상기 실시예 1과 같이 본 발명을 실시하되, 저온처리 단계와 고온처리 단계의 반복 처리를 형광등이 존재하는 일장하에서 처리하였다.The present invention was carried out as in Example 1, but repeated treatment of the low temperature treatment step and the high temperature treatment step was performed under the presence of fluorescent lamps.
실험결과는 도 2에서와 같이 유식물체가 엽록소 형성을 보였는데 이와같은 결과를 일장 및 버널리제이션(Vernalization) 효과에 의해 엽록소의 발현과 관계하는 유전자의 발현이 유기된 것으로 관찰되었다.Experimental results showed that the seedlings showed chlorophyll formation as shown in FIG. 2, and it was observed that the expression of genes related to chlorophyll expression was induced by the work and vernalization effects.
이와 같은 실시예 1과 실시예 2의 유식물의 차이는 고려인삼종자 발아 식물의 약리 활성에도 큰 영향이 있을 것으로 판단되므로 약리성분들의 정성·정량 분석의 필요성을 인식시켜주고 있다.Since the difference between the seedlings of Example 1 and Example 2 is expected to have a significant effect on the pharmacological activity of the germinated plant of Korean ginseng seed, it recognizes the need for qualitative and quantitative analysis of pharmacological components.
한편, 본 발명에서 상기 실시예 1 및 실시예 2에서 저온에서 상대습도를 60%이상 또는 그 이하로 하여도 좋으나 유식물 성장에 가장 바람직 하기는 60%에서 -5℃였다. 또 고온처리 단계에도 90% 조건에서 20℃에서 가장 바람직한 결과를 보였다. 따라서 처리온도 및 상대습도의 수치변경은 당업자 수준에서 다수의 실험에 의해 변경이 가능하다.On the other hand, in the present invention in Example 1 and Example 2, the relative humidity at a low temperature may be 60% or more or less, but most preferably for planting growth was -5 ℃ at 60%. In addition, the high temperature treatment step showed the most desirable result at 20 ° C. at 90%. Therefore, the numerical change of the treatment temperature and the relative humidity can be changed by a number of experiments at the level of those skilled in the art.
이상 실시예를 통하여 명백한 바와 같이 본 발명은 지금까지 알려지지 않았던 고려인삼종자의 세계최초 발아식물을 제공하는 효과가 있으므로, 본 발명에서 얻은 식물성장조절제나 항진균제 또는 항생제가 처리되지 않은 인체 및 도축에 무해한 발아유식물을 이용하여 인체에 유용한 식품, 의약물 개발산업에 유용할 뿐만 아니라 향장분야 산업상 매우 유용한 발명인 것이다.As is apparent from the above examples, the present invention has an effect of providing the world's first germinated plant of Korean ginseng seed, which has not been known so far, and is harmless to humans and slaughterhouses not treated with the plant growth regulators, antifungal agents or antibiotics obtained in the present invention. Germinated seedlings are not only useful for food and drug development industries useful for the human body, but are also very useful inventions for the cosmetic industry.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101042545B1 (en) | 2008-06-24 | 2011-06-20 | 박동준 | method of cutivation for ginseng |
| KR101095966B1 (en) * | 2006-06-16 | 2011-12-20 | 최용조 | Asceptic germination method of Korea ginseng seed |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100828587B1 (en) * | 2007-11-26 | 2008-05-09 | (주)청구바이오 | Dehisced method for ginseng seed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101095966B1 (en) * | 2006-06-16 | 2011-12-20 | 최용조 | Asceptic germination method of Korea ginseng seed |
| KR101042545B1 (en) | 2008-06-24 | 2011-06-20 | 박동준 | method of cutivation for ginseng |
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