KR100487103B1 - Pharmaceutical Preparation for Treatment of Dermatoses Containing Silver Compound as Effective Ingredient - Google Patents

Abstract

The present invention relates to a preparation for treating skin diseases and a method for producing a skin disease containing a silver compound as an active ingredient for the prevention and treatment of microbial infections such as molds, bacteria, viruses, or a cause of skin diseases. The silver compound contained in the skin disease treatment preparation according to the present invention is prepared by dissolving an organic or inorganic salt of silver in water. Preparations for the treatment of skin diseases prepared according to the invention are prepared in the form of solutions, gels, ointments, creams and the like. According to the preparation according to the present invention, it is possible to achieve a significant skin disease treatment effect that significantly reduces and further eliminates various fungi, bacteria, viruses, but does not cause resistance to the use of the preparation.

Description

Pharmaceutical Preparation for Treatment of Dermatoses Containing Silver Compound as Effective Ingredient}

The present invention relates to a preparation for treating skin diseases containing a silver compound as an active ingredient for the prevention and treatment of microbial infections such as mold, bacteria, viruses, or a causative skin disease, and a method for producing the same. More specifically, the present invention provides a preparation for the treatment of skin diseases containing silver compounds as an active ingredient, which significantly reduces the fungus, bacteria and viruses and, as a result, effectively eliminates them and does not develop resistance even after continuous use. It is about.

Human diseases come from internal or physical or biological external causes, such as breakdowns or instability of physiology and metabolism. Among them, the biological causes such as fungi, bacteria and viruses are removed in several ways. There are ways to keep the number of fungi, bacteria, and viruses from increasing, or kill each organism. Preservatives, fungicides, and disinfectants are used. As preservatives, fungicides and disinfectants, mercury organic compounds, iodine solutions, alcohols, phenol compounds, hydrogen peroxide, surfactants, mercury chloride, copper sulfate, chlorine gas, chlorine compounds, ozone, ethylene oxide and the like are used. Among them, human peroxides are hydrogen peroxide, iodine solution, the rest are inadequate due to strong toxicity or pain in use. On the other hand, iodine solution is not used on the face or other exposed areas because of its unique red color. Although hydrogen peroxide is widely used, relatively high concentrations are required for effective action.

Antibiotics are widely used to inhibit the growth of fungi, bacteria and viruses. Antibiotics are mostly organic compounds and are extracted from organic synthesis or culture medium such as molds and bacteria, and extract and separate antibiotic components from plants. However, the abuse of antibiotics has made many pathogenic bacteria resistant to antibiotics and therefore requires the development of new antibiotics. Resistance to antibiotics is manifested by the emergence of strains with the ability to modify organic compounds, which continues the cycle of development of new antibiotics and the development of new strains.

For this reason, it is necessary to disinfect pathogenic bacteria, to treat them at low concentrations, and to have a bactericidal substance that is harmless to the human body. It is also more effective if one substance removes a wide range of actions, such as mold, bacteria and viruses. Fungi are eukaryotes, and most of the fungal metabolism is similar to humans. However, the fact that the fungus has a cell wall and the cholesterol metabolism process is slightly different, the substances inhibiting this part are being synthesized. For bacteria, treatment with antibiotics may not be a major problem if many antibiotics are made or separated to resolve the problem of resistance. On the other hand, since the virus proliferates after invading the cells, the focus is on the development of drugs that rely on immune cells in the human body to kill virus-infected cells or to inhibit virus-specific metabolic processes.

As it is found that silver is excellent for sterilization and antibacterial, researches using silver in various fields are being conducted, and techniques have been proposed. For example, Korean Patent Application Publication No. 1999-69905 (published on September 6, 1999) discloses a therapeutic article for treating a skin disease by attaching at least one of silver, copper, or a compound thereof to a flexible material and then contacting the affected area. Is proposed. However, the present invention is formed of silver or copper in a metallic state in the form of small particles or membranes on a flexible material, and its bactericidal or antimicrobial effect is very insignificant. It appears to be because silver does not act directly at the disease site.

Meanwhile, another invention using silver bactericidal properties is the invention of Korean Patent Application Publication No. 2000-73636 (published Dec. 5, 2000), which is an antimicrobial agent using silver compounds for use in preventing corruption in food or feed. , An antifungal solution and a method for producing the powder are proposed. This invention is proposed to prevent the decay of food or feed by preparing a compound containing silver and coating it on a wrapping paper or gauze or adding it to a feed, which indirectly uses the antibacterial and bactericidal properties of silver.

Similarly, Korean Patent Application Publication No. 2001-54432 (published Jul. 2, 2001) also proposes an attempt to express antimicrobial activity on a fabric by preparing a silver compound and an antimicrobial composition containing the same and applying the same to a fabric. have. Of course, the main focus of the present invention is to suppress the discoloration by light even when the silver compound is applied to a fabric, particularly a fabric made of a polymer material such as polyurethane, polyamide, polyvinyl alcohol, cellulose, wool, polyester, or the like. .

As described above, the antimicrobial and bactericidal properties of silver or silver compounds have been applied to wrapping paper or fabrics, or metals have been indirectly applied to skin diseases, but more effective treatment effects for skin diseases caused by bacteria, fungi, viruses, etc. And attempts to achieve resistance resistance are still insignificant.

Examples of various diseases caused by fungi, bacteria, and viruses include early germ line, femoral ringworm, fungal alopecia, herpes viral herpes, acne, eczema, contact dermatitis, and various skin fungi. Infection treatment of ringworm is treated by taking medicines such as Griseofulvin for 3-5 weeks for 1g daily. Topical therapies include many external preparations such as salicylic acid, resorcinol and phenol, and external application of chemical preparations such as tolnaftate, chlorotrimazole myconazole, and econazol. Acne treatment is a topical therapy that removes facial fat by washing face, open pores so that contents can be easily discharged from cotton cloth, and degreasing agent (alcohol, propylene) that removes skin fat with external medicine (mostly lotion type). Recalls) or keratin solubilizers (sartic acid, sulfur, resorcin, sodium thioglycol, etc.). In addition, there are antimicrobial agents that can kill or weaken the acne bacteria in the skin surface, pores, and follicles (crimmycin, erythromycin, kronalphenicol, etc.). Systemic therapies include antibiotics, direct injection into cystic acne, and injections such as inflammation inhibitors (steroids). Vitamins are also effective for treating acne. Scars are undergoing plastic surgery.

However, the conventional treatments as described above require a lot of patience and effort to obtain side effects as well as the treatment effect, and patients do not respond to this because they tend to be easily treated with simple drugs.

In order to effectively prevent or treat skin diseases as exemplified above, the present inventors solve the problem of resistance of antibiotics as described above in connection with the prevention and treatment of skin diseases caused by bacteria, fungi, viruses, etc. Experiments have shown that it is possible to adjust the concentrations of organic silver salts and inorganic silver salts (which are described in detail in the description of the present invention), thereby completing the present invention.

Accordingly, an object of the present invention is a preparation for treating skin diseases containing a silver compound as an active ingredient for preventing and treating microbial infections such as fungi, bacteria, viruses, or a causative skin disease without resistance, and It is for providing a manufacturing method.

In addition, an object of the present invention is to provide a therapeutic effect by simply applying or attaching to various skin diseases caused by mold, bacteria, and viruses, and it is a formulation containing a silver compound which is harmless to the human body. To provide in the form of a cream, patch (patch), cosmetics and the like.

Another object of the present invention is to obtain a silver compound in a ready-to-use state without going through an inconvenient and secondary manufacturing process, such as devices for producing a silver compound and an electrolysis process to provide as a raw material of the agent for the treatment of skin diseases It is for.

In accordance with the above object, the present inventors have been able to identify which silver compound is effective in removing mold, bacteria, and viruses, and have confirmed the effective concentration and time of the silver compound. The bactericidal properties were confirmed to complete the present invention.

According to the present invention, a silver compound-containing skin disease treatment agent is provided, which comprises a silver compound as an active ingredient.

According to another aspect of the present invention, there is provided an agent for treating skin diseases of liquid, gel, ointment, and cream type containing 5 to 100 ppm of silver compound in a weight ratio concentration according to the type of silver compound selected.

Examples of the silver compound include silver salts such as silver acetate, silver carbonate and silver phosphate; Silver organic salts such as silver lactate, silver malic acid salt, silver amino acid salt, silver nucleate salt and silver retinoic acid salt; Silver chloride, silver bromide, silver iodide, silver sulfate, silver nitrate, etc. can be selected from silver inorganic salts. The active ingredient in the preparation for treating skin diseases according to the present invention consists of a silver organic salt or a silver inorganic salt in the form of a salt with a counterbase or an inorganic anion of a silver organic acid. In addition to the liquids, gels, ointments and creams, the enamels, resins, plasters, powders, aerosols and sprays may be used. It is, of course, also possible that the formulations of any form as exemplified above with the silver salts, silver organic salts or silver inorganic salts as an active ingredient may be prepared in the form of a band-aid, bandage or paste coated or coated. However, these results are merely based on the effects and easy applicability of the embodiments of the present invention, and may be used as more various results using the gist or characteristics of the present invention.

Each of these preparations is realized by dissolving using distilled water and 0.1 to 2% by weight of surfactants (specifically described in the examples) and mixing them in a base of the type to be prepared for subsequent processing ( Detailed description thereof will be made in the embodiment).

In the present invention, the silver compounds which have most preferably effected are silver acetate, silver carbonate and silver phosphate. As will be described later in detail, it has been demonstrated by the present inventors that the silver acetate, silver carbonate and silver phosphate have an effective bactericidal activity even at a low concentration and in a short time. That is, silver carbonate, silver acetate, and silver phosphate among the silver compounds, which are the key components of the present invention, showed particularly excellent antibacterial, antiviral and antifungal effects, and the concentration of 10 ppm was suitable for bacteria. When it is necessary to set it as low concentration 5 ppm, the effect can be acquired by taking the means of lengthening contact time with a silver compound liquid formulation. Here, the ointment is suitable as a means to use at a low concentration and can be applied to the form of gel (gel) or plasters or plasters. If a shorter prescription is required, a concentration of 100 ppm may be used. Here, as a means to use at a high concentration, the liquid or lotion form which is easy to wash off after use is suitable. On the other hand, human toxicity by the preparations according to the present invention did not appear at a significant level, the stability thereof was confirmed. Regarding the concentration, bactericidal effects can be obtained even at low concentrations of 5 to 10 ppm in the case of bacteria, and growth can be completely inhibited even at low concentrations of 100 ppm in the case of fungi.

In the above description of the main technical idea of the present invention, silver carbonate, silver acetate, silver phosphate in common components are silver, antimicrobial and bactericidal effect against viruses, bacteria, fungi is represented by silver ions. In addition, the inventors considered in relation to their human applicability, because carbonic acid, acetic acid, and phosphate ions are naturally present in vivo, and therefore cannot be toxic. Moreover, since silver compounds destroy proteins by precipitating or denaturing them, in addition to the antimicrobial and bactericidal effects on viruses, bacteria, fungi, etc., mechanisms that these microorganisms are not resistant to are realized. Important considerations by the present inventors. Based on such a reason, all silver compounds can be used as a preparation for skin disease treatment, Especially silver salts, such as silver acetate, silver carbonate, silver phosphate; Silver organic salts such as silver lactate, silver malic acid salt, silver amino acid salt, silver nucleate salt and silver retinoic acid salt; Any one selected from silver inorganic salts such as silver chloride, silver bromide, silver iodide, silver sulfate and silver nitrate can be used as an active ingredient of the agent for treating skin diseases of the present invention.

On the other hand, when the silver compound of the form of salts are selected together with the antibiotic having a negative charge has a synergistic effect by the effect of silver ions and the effect of antibiotics. Specifically, the sterilization effect is only exerted by the action of silver ions when using silver organic salts or silver inorganic salts, but when an anion binding to silver ions is composed of a bactericidal or antimicrobial substance, bacteria, viruses and fungi The ability to kill comes from silver ions and their counterparts, which increases their effectiveness. One example is sodium ampicillin salts, which are replaced by silver ions to silver ampicillin salts, where ampicillin anions are antibiotics that inhibit bacterial cell wall synthesis. Excellent sterilization and antimicrobial effect.

Hereinafter, test examples and examples of the present invention will be described.

First, the antimicrobial, antiviral, and antifungal effects of the silver compound are identified through the test examples of the present invention, and the emulsion, suspension, or ointment (emulsion) or suspension (suspension) containing the silver compound is shown in Examples. ointment), creams, patches, etc., and the results of the performance tests are described.

[Test Example 1]

In this test example, a liquid preparation containing silver carbonate was prepared, and the antibacterial effect thereof was revealed.

The silver carbonate solution was dissolved in 5ppm and 10ppm concentrations in distilled water, and then added to E. coli. After 2.5 minutes and 15 minutes, a certain amount of suspension was taken and diluted. The mixture was mixed with 45 ° C of dissolved solid medium and poured into a culture dish. The cells were incubated at 37 ° C. until bacterial colonies were counted. In the control experiment, the number of pathogenic bacteria was determined after treatment under the same conditions as the experimental group using a 10 ppm sodium carbonate solution. More than 14,800 bacterial populations were observed in the control group (for Staphylococcus epidermis bacteria), but most pathogenic bacteria did not grow in the experimental group treated with silver carbonate solution. This demonstrates that silver carbonate has an effective bactericidal activity in a short time despite low concentrations. The results are shown in [Table 1].

   <Bactericidal Activity of Pathogenic Bacteria in Silver Carbonate Solution> Remarks Control Experimental group 10 ppm sodium carbonate solution 5 ppm silver carbonate solution 10 ppm silver carbonate solution 2.5 minutes 15 minutes 2.5 minutes 15 minutes E. Coli BL21 59300 One 0 0 0 Pseudomonas genus 118000 2 0 One 0 Proteus genus 74000 0 0 0 0 Salmonella genus 69200 0 0 0 0 E. Coli NIHJ 40500 One 0 0 0 E. aerogenes 19200 One 0 0 0 Vibrio fluvialis 37600 0 0 0 0 Staphylococcus aureus 50800 2 0 0 0 Staphylococcus epidermis 14800 0 0 0 0 Propionbacterium acnes 159000 One 0 0 0

(Note) 1. Experimental group: Number of bacterial colonies after 2.5 or 15 minutes treatment at a concentration of 5 ppm or 10 ppm

     2. Control group: Bacterial colonies grown after 15 minutes treatment with 10ppm sodium carbonate solution

[Test Example 2]

In this test example, a liquid preparation containing silver acetate was prepared and its antibacterial effect was revealed.

The silver acetate solution was made into 5 ppm and 10 ppm concentrations in distilled water, and after 5 and 15 minutes of administration of pathogenic bacteria, the solution was diluted with a certain amount of suspension, mixed with 45 ° C of a solid medium, and poured into a culture dish. The cells were incubated at 37 ° C. until bacterial colonies were counted. In the control group, 10 ppm sodium acetate solution was used to determine the number of pathogenic bacteria after treatment under the same conditions as the experimental group. In the control group, a bacterial colony of 17,200 (for this collie bacterium) to 91,400 (only for propionibacterium bacteria) was observed, but a very small number of pathogenic bacterial colonies survived in the experimental group treated with silver acetate solution. This demonstrates that silver acetate has an effective bactericidal activity in a short time despite low concentrations. The results are shown in [Table 2].

    <Bactericidal Activity of Pathogenic Bacteria in Silver Acetate Solution> Remarks Control Experimental group 10 ppm sodium acetate solution 5 ppm silver acetic acid solution 10 ppm silver acetic acid solution 2.5 minutes 15 minutes 2.5 minutes 15 minutes E. Coli BL21 17200 5 2 One One Pseudomonas genus 74900 2 One 0 0 Proteus genus 99400 3 One One 0 Salmonella genus 83700 5 One 2 One E. Coli NIHJ 63100 2 One One One E. aerogenes 47500 One 0 0 0 Vibrio fluvialis 64500 2 One 0 0 Staphylococcus aureus 73200 One 0 0 0 Staphylococcus epidermis 62300 3 One One 0 Propionbacterium acnes 91400 2 0 0 0

(Note) 1. Experimental group: Number of bacterial colonies after 5 or 15 minutes treatment at a concentration of 5 ppm or 10 ppm.

     Control group: bacterial colonies grown after 15 minutes treatment with 10 ppm sodium acetate solution

[Test Example 3]

In this test example, a liquid preparation containing silver phosphate was prepared, and the antibacterial effect thereof was revealed.

The silver phosphate solution was made into 5ppm and 10ppm concentrations in distilled water, and after 5 minutes and 15 minutes of pathogenic bacteria were added, the solution was diluted with a certain amount of suspension, mixed with 45 ° C of the dissolved solid medium, and poured into a culture dish. The number of cultures was counted until bacterial colonies emerged at 37 ° C. In the control group, 10 ppm sodium phosphate solution was used to determine the number of pathogenic bacteria after treatment under the same conditions as the experimental group. In the control group, more than 14,300 bacterial colonies (for Staphylococcus epidermis bacteria) were observed, but only a small number of bacteria survived in the experimental group treated with silver phosphate solution. This demonstrates that silver phosphate has an effective bactericidal activity in a short time despite low concentrations. The results are shown in [Table 3].

   <Bactericidal Activity of Pathogenic Bacteria in Silver Phosphate Solution> Remarks Control Experimental group 10 ppm sodium phosphate solution 5 ppm silver phosphate solution 10 ppm silver phosphate solution 2.5 minutes 15 minutes 2.5 minutes 15 minutes E. Coli BL21 88900 2 0 0 0 Pseudomonas genus 81400 0 0 0 0 Proteus genus 75300 2 0 One 0 Salmonella genus 58000 One 0 0 0 E. Coli NIHJ 93500 4 0 0 0 E. aerogenes 65600 2 0 0 0 Vibrio fluvialis 107000 One One 0 0 Staphylococcus aureus 127000 0 0 0 0 Staphylococcus epidermis 14300 2 0 0 0 Propionbacterium acnes 101000 0 0 0 0

(Note) 1. Experimental group: Number of bacterial colonies after 5 or 15 minutes treatment at a concentration of 5 ppm or 10 ppm

     Control group: bacterial colonies grown after treatment with 10 ppm sodium phosphate solution for 15 minutes

[Test Example 4]

This test example reveals the antiviral effect of silver compounds.

The host E. coli to be used for the experiment was grown in 100 mL liquid medium containing 0.2% maltose and centrifuged to collect the E. coli, and then solved in 10 mM magnesium sulfate solution. Lambda (λ) virus was diluted appropriately in a buffer solution, exposed to 100 ppm silver acetate solution for 5 minutes, and then diluted for 30 minutes. Thus prepared E. coli and virus were mixed and incubated at 37 ° C. for 20 minutes to infect the E. coli virus, mixed with 0.7% agar medium at 48 ° C., and cultured at 37 ° C. on a hard agar medium. The effect of the silver compound was counted by counting the viral colonies generated after 12 hours. 100 ppm sodium acetate solution was used as a control.

69 or 894 viral colonies were observed in the control group, while fewer viral communities were observed in the experimental group treated with silver acetate solution. In particular, virus inactivation of 58-62% in the experimental group treated for 5 minutes and 21-25% in the experimental group treated for 30 minutes was observed. This demonstrates that silver compounds have effective virus inactivation properties. The results are shown in [Table 4].

   <Antiviral Effect of Silver Compounds> process Control Experimental group 100 ppm sodium acetate solution 100 ppm silver acetic acid solution 5 minutes 30 minutes Viral colony (dog) (10-fold dilution) 69 43 17 Virus colony () 894 521 184 efficiency(%) 60 23

  (Note) 1. Experimental group: After exposing the virus for 5 minutes or 30 minutes in 100 ppm silver acetate solution (by the method according to the virus colony count), the activity of the virus was measured.

       Control group: colony of virus grown after treatment with 100 ppm sodium acetate solution for 30 minutes

[Test Example 5]

This test example reveals the inactivation of mold by silver compounds.

A solid medium containing 0, 12.5, 50, 100, 200 ppm of silver acetate was prepared and inoculated with the mycelia in culture, and 4 days later, the degree of mycelia growth was examined. The concentration of 12.5ppm did not show a significant difference from the control group, but the growth was slightly lowered at 25ppm, and as the concentration increased, the mycelial growth was clearly reduced (see FIG. 1). This demonstrates that silver compounds effectively inactivate fungi. The result of this test example is shown in FIG.

Subsequently, preparations for the treatment of skin diseases containing silver compounds are prepared in various types and the antimicrobial effects against pathogenic bacteria are thereby revealed through the examples.

Example 1

In this example, a liquid formulation containing silver acetate as a component was prepared. First, dissolve silver acetate at a concentration of 10 ppm in distilled water and add 0.1% of the surfactant Tween 80 (product name of ICI Americas Inc., Wilmington, Delaware). It was. Here, in the case of silver acetate, since the maximum concentration of the aqueous solution that can be used in pairs with silver ions is 10,000 ppm, the concentration of silver acetate in the preparation of the liquid formulation may be as low as 5 ppm, as much as 10,000 ppm. On the other hand, using silver carbonate or silver phosphate instead of silver acetate was prepared in the same manner as above, but the description thereof is omitted for convenience. However, silver carbonate could dissolve up to about 33ppm when dissolved in water, and silver phosphate could melt up to 65ppm.

After 2.5 minutes, 5 minutes, and 15 minutes, various bacteria were added to the prepared solution, and a certain amount of suspension was taken and diluted. Then, the mixture was mixed with the melted solid medium at 45 ° C. and poured into a culture dish. The cells were incubated at 37 ° C. until bacterial colonies were counted. The number of pathogenic bacteria in the comparative example was determined by treatment under the same conditions as the experimental group using a 10 ppm sodium acetate solution. The silver compound, i.e., the solution containing silver acetate, had an effective bactericidal activity at low concentration and in a short time. In addition, Tween 80, a surfactant, will enhance the wetting properties of liquids on skin, other body parts, or other media, thereby increasing the effectiveness of removing infectious bacteria, viruses, and fungi. The results are shown in [Table 5].

<Antimicrobial performance test on pathogenic bacteria in solution containing silver compound (silver acetate)> Remarks Control Experimental group 10 ppm sodium acetate 0.1% Tween 8010ppm Silver Compound 15 minutes 2.5 minutes 5 minutes 15 minutes Pseudomonas genus 78100 0 0 0 Proteus genus 14700 0 0 0 Salmonella genus 62800 0 0 0 E. Coli NIHJ 118000 0 0 0 E. aerogenes 38300 0 0 0 Vibrio fluvialis 126000 0 0 0 Staphylococcus aureus 64900 0 0 0 Staphylococcus epidermis 134000 0 0 0 Propionbacterium acnes 33100 0 0 0

(Example) 1. Example: 2.5 minutes, 5 minutes or 115 minutes of each bacteria in a solution containing 0.1% Tween 80, 10ppm silver compound

     Comparative Example: Bacterial colonies grown after treatment for 15 minutes with 10 ppm sodium acetate solution

Example 2

In the present embodiment, an ointment having a composition ratio as shown in [Table 6] below containing 1 wt% (10,000 ppm by concentration) of silver compound, for example, silver acetate, is prepared. Mineral oil, mystic acid isopropyl ester, polyethylene 40 stearate, and Tween 80 are composed in the composition ratio as shown in [Table 6] and dissolved in 75 ℃. The temperature is kept at 60-65 degreeC, stirring, pouring 75 degreeC distilled water here. In parallel with this, polyethylene glycol 200, silver acetate and distilled water are mixed to dissolve the silver compound, i.e. silver acetate, which is poured into the vessel and stirred to homogenize while maintaining the same temperature as the stirring temperature, and cooled to room temperature. Harden to complete the ointment.

Here, as in Example 1, in the case of silver acetate, the maximum concentration of the aqueous solution that can be used in pairs with silver ions is 10,000 ppm, so that the concentration of silver acetate in the preparation of the liquid solution can be as low as 5 ppm and as much as 10,000 ppm. have. On the other hand, using silver carbonate or silver phosphate instead of silver acetate to prepare a liquid in the same manner as above, the description thereof will be omitted for convenience. However, silver carbonate could dissolve up to about 33ppm when dissolved in water, and silver phosphate could be dissolved up to 65ppm.

It should be noted that the composition ratio of Table 6 is shown as an example, and this composition ratio was obtained in the following antibacterial performance test even if the composition ratio was adjusted within the composition range as shown in Table 7 below. Of course, the composition ratio of [Table 6] falls within the composition range of [Table 7].

<Component Weight Ratio of Ointment Containing Silver Acetate According to the Present Example> ingredient content(%) Distilled water 42 Polyethylene Glycol 200 7.8 Mineral oil 16 Styryl alcohol 17 Twin 80 1.1 Polyethylene 40 Stearate 8.9 Mystic Acid Isopropyl Estee 6.6 Silver acetate 1.0

<Component range of ointment containing silver compound possible according to the present invention (unit: wt%)>   Polyethylene Glycol 200 7% to 40%   Mineral oil 1% to 25%   Styryl alcohol 7% to 45%   Twin 80 0.1% to 2%   Polyethylene 40 Stearate 5% to 20%   Mystic Acid Isopropyl Ester 0% to 15%   Silver compound 5 ppm to 10,000 ppm   Distilled water Cup wealth

Example 3

7.7 g polyethylene glycol 200 and 3.9 g polyethylene glycol 8000 were dissolved and 1.1 mL of water was added thereto to prepare an ointment medium. In 1.1 mL of water, some amounts of silver compounds were dissolved within the range according to the present invention. With this, ointment was prepared in the same manner as in [Example 2].

Example 4

In this embodiment, a gel containing a silver compound was prepared using (1) polysaccharide or gelatin as a medium, or (2) polyacrylamide as a medium.

(1) Method of using polysaccharide or gelatin as a medium

Select starch from polysaccharides such as agar, agarose, starch, or carbohydrate, and dissolve 20 g (using 30 g gelatin as an example) in 500 mL of distilled water. Cool it down to 50 ° C. 10 g of silver compound (silver carbonate, silver acetate, silver phosphate are applied as separate examples) and 20 g of Tween 80 are dissolved in 500 mL of distilled water and heated to a temperature of 50 ° C., and then poured into a container in which the polysaccharide is dissolved. Cooled by mixing homogeneously.

(2) Method of using polyacrylamide as a medium

15 g of acrylamide monomer and 0.3 g of bisacrylamide are dissolved in 100 mL of distilled water. 1 mL of 10% by weight of ammonium persulfate and 0.8 mL of thymid are added to start the polymerization reaction and poured into a container to make a gel completely. The hardened gel is placed in a container containing 1 L of distilled water and stirred well for 1 hour. This stirring is carried out three to four times with fresh distilled water, followed by one hour in 10 liters of silver compounds (silver carbonate, silver acetate, silver phosphate are used as separate examples) and 1 liter of distilled water in which 20 g of Tween 80 is dissolved. It was left to stand and taken out to prepare a gel containing a silver compound.

As a result of the test by adjusting each of the components in the two methods in various ways, the composition ratio showing the antimicrobial effect was determined as shown in Table 8 below.

                                                  (Unit: weight%)   Silver compound 5pp to 10,000ppm   Twin 80 0.1% to 2%  Medium   Polysaccharides such as agar, agarose, starch or carbohydrates 0.5 to 2%   Polyacrylamide 5% to 15%   gelatin 1% to 3%

Example 5

In this example, a formulation containing a silver compound was prepared in the form of a cream.

It was prepared in each of the examples while adjusting the composition ratio of the components in various ways, and the antibacterial performance test with each of them to achieve the level intended in the present invention. The range which adjusted the component is shown in the following [Table 9].

Briefly describing the manufacturing process, mineral oil, microcrystalline wax and lanolin, stearic acid polyoxyethylene ester were placed in a container and dissolved by heating to 75 ° C. so as to maintain an optional component within the range of [Table 9]. Water is maintained at 60 to 65 ° C. while stirring and stirring to maintain 75 ° C. Propylene glycol, a silver compound (silver carbonate, silver acetate, silver phosphate are applied as separate examples) and water are mixed to dissolve the silver compound, which is poured into the vessel and stirred homogeneously while maintaining the temperature at 60 to 65 ° C as well. After that, the cream was prepared by cooling at room temperature.

                                            (Unit: weight%)   Microcrystalline wax 10% to 25%   Vegetable oil 30% to 45%   lanolin 2% to 10%   Propylene glycol 5% to 10%   Stearic Acid Polyoxyethylene Ester 2% to 10%   Twin 80 0.1% to 2%   Silver compound 5 ppm to 10,000 ppm   Distilled water Cup wealth

[Antibacterial Performance Test]

Each of the silver compound-containing preparations (liquids, ointments, gels, and creams) prepared by adjusting the amount of the silver compounds therein, prepared in Examples 1 to 5, respectively, and pathogenic bacteria Antimicrobial performance against was measured. In other words, the antibacterial activity against the pathogenic bacteria Staphylococcus aureus and Staphylococcus epidermis and acne causing bacteria Propionibacterium acnes were measured.

In order to measure the bactericidal effect of the formulations in the above examples containing silver compounds, E. coli was spread on medium X containing sodium chloride and medium Y without sodium chloride, and the formulation was dropped or applied thereon, followed by culturing at 30 ° C. The growth of bacteria was observed. The concentration of the silver compound is expressed in ppm in the amount contained in the formulation having their representative properties. The results are shown in [Table 10]. As can be seen from Table 10, when silver carbonate is contained as an example, the growth of Escherichia coli was suppressed even at the minimum concentration of 33 ppm used. The lower part of the solution, ointment, gel, or cream was transparent because E. coli could not grow.

<Antimicrobial Performance Test on Pathogenic Bacteria and Acne-causing Bacteria from Silver Containing Agents> Remarks Sodium chloride containing medium (X) Medium without sodium chloride (Y) A B C A B C compare 1000 ppm sodium acetate - - - - - - Example 33 ppm silver carbonate + + + + + + 66 ppm silver phosphate + + + + + + 200 ppm silver acetate + + + + + + 2000ppm silver acetate + + + + + +

(Note) comparative example is ointment containing 1000ppm sodium acetate

      "-" Indicates no bactericidal effect, "+" indicates bactericidal effect

      A: Staphylococcus aureus

      B: Staphylococcus epidermis

      C: Propionibacterium Acnes

The above test examples and examples were all made in a test tube or a culture dish, but the results of the present invention were used to demonstrate the therapeutic effect of the chronic and refractory dyscholine tinea in humans, and no other side effects were observed. Conventional treatments for ringworm require considerable effort and time for complete cure, such as regular blood tests and liver function tests, along with continuous use of medications such as griseofulvin for three to six months. However, using the result of the present invention can be treated periodically without going to the hospital and blood test or liver function test.

In addition, as shown in the embodiment of the present invention, it can be seen that the inactivation of the causative agent Proionibacterium acnes (Propionibacterium acnes). Therefore, in the case of acne, using the preparation for treating skin diseases according to the present invention, the treatment is facilitated by the process of applying directly to the acne affected area without any psychological burden, and of course, the number of acne-causing bacteria can be drastically reduced. Can effectively cure

As in the test examples and the examples of the present invention, bacteria, fungi, and viruses were inactivated using a small amount of silver compound-containing preparation within a short time and human-safe range.

The result of the present invention can replace the ointment or liquid containing the antibiotic used conventionally. At the time when resistance to antibiotics is currently found in many pathogens, the agent for treating skin diseases based on the silver compound according to the present invention is not changed by living organisms, and its efficacy may be sustained. That is, resistance to antibiotics of pathogens is caused by obtaining genetic traits that can modify antibiotics, which are organic compounds. Since silver is not an organic substance, it can be affected by an inert method on organic compounds. none. In addition, the effect is so rapid that there will be no room for inactivation.

Many antibiotics have a drawback in that they take effect over a certain concentration of pathogens for a long time or applied to the affected area. However, the silver compound-containing skin disease preparation according to the present invention inactivates pathogens within about 5 minutes. Therefore, various adverse effects such as impaired liver function caused by long-term antibiotic use can be reduced.

1 is a photograph showing the degree of growth of mycelia after 4 days of inoculation with mycelia under cultivation by making a solid medium containing silver acetate (CH3COOAg) as a result of examining the antifungal performance of the silver compound.

Claims (8)

delete In the agent for the treatment of skin diseases containing a silver compound as an active ingredient, The silver compound is any one of silver carbonate, silver acetate and silver phosphate (silver phosphate) in the state of the silver salt, formulation containing a silver compound-containing skin disease, characterized in that it contains 5 to 10,000ppm . The agent for treating skin diseases containing silver compound according to claim 2, wherein the concentration of silver carbonate is 5 to 33 ppm. The agent for treating skin diseases containing silver compound according to claim 2, wherein the concentration of silver acetate is 5 to 100 ppm. The agent for treating skin diseases containing silver compound according to claim 2, wherein the concentration of silver phosphate is 5 to 65 ppm. delete The preparation for treating a skin disease containing silver compound according to any one of claims 2 to 5, wherein the preparation is prepared in the form of a liquid, gel, ointment, or cream. 8. The silver compound-containing skin according to claim 7, wherein the preparation is applied to any one of ringworm, leprosy, fungal alopecia, herpes viral herpes, acne, eczema, contact dermatitis and dermatitis. Formulations for the treatment of diseases.