KR100456827B1 - collecting device for aquatic microscopic-life - Google Patents

Abstract

The present invention relates to a collecting device for collecting aquatic microorganisms, each of which has a plurality of collection containers, each of which is open at the top and has a plurality of axial slots, into which a porous foam is introduced, and a central support shaft having connecting portions at both ends thereof; A lower plate on which the collecting container is raised in an upright position, a lower frame for supporting the lower plate, an upper plate provided with one or more entrance holes, and an upper frame positioned in a form surrounding an edge thereof to allow rotation of the upper plate; The intermediate plate is disposed between the lower plate and the upper plate and has a plurality of fixing holes for fixing the collection container one by one, and a configuration including an intermediate frame coupled with the intermediate array plate, in the current and current Effects directly on porous foams such as polyurethane foams used for microbial collection To minimize the damage and to facilitate handling during the collection process, thus enabling stable and effective microbial collection, and furthermore, the results of the assessment of pollution and the health of marine ecosystems through microbial community analysis. It provides an aquatic microbial collecting device having an effect of improving the reliability of the.

Description

Collecting device for aquatic microscopic-life

The present invention relates to a collecting device for collecting aquatic microorganisms, and more particularly, to enable effective microbial collection for microbial community analysis performed to evaluate the degree of pollution and health of the ocean by a biological method. It relates to an artificial habitat-type aquatic microbial collecting device.

In general, in evaluating the environment of the ocean, water quality is measured by using chemicals such as dissolved oxygen measurement to evaluate the degree of pollution in various sea areas. However, the measurement of water quality by physicochemical water quality methods is often unsuitable for evaluating the degree of marine pollution or the health of marine ecosystems. In order to evaluate the health of marine ecosystems can be used to test the toxicity of some species, but this is also unfavorable because the problem of the selection of species and inconsistency of the standards according to the resistance is pointed out as a problem.

As an alternative to the above problems, in order to evaluate the health and pollution of marine ecosystems, in addition to measuring physicochemical water quality factors, biological organisms that want to assess the health of marine ecosystems by analyzing the community status of microorganisms such as protozoa An approach has been proposed.

This method collects and analyzes microbial communities that exist at the top and bottom of aquatic ecosystems and are sensitive to environmental changes.

For example, a form that has been applied to fresh water, a collecting time including a porous foam, such as polyurethane foam that can be a habitat for microbial communities is put into the water to be inspected, a predetermined time may be several days After this, the collecting means is collected, the method is performed by squeezing the porous foam outside the water to separate the microorganisms present in the form contained therein, and then collecting the microorganisms. This has been used.

Porous foam is provided as an artificial habitat for collecting microbial communities in water masses. In general, the porous foam is placed in a target water by putting a bag-shaped mesh freely out of the water into a large mesh to form a microbial clustered in the water. Used to collect. Polyurethane foams used herein have suitable properties as habitats for most microbial communities, including planktonic communities, periphytonic communities, and benthic protozoa communities.

In the 1970's, Carins et al. Developed microbial collection methods using polyurethane foam as described above to test the degree of contamination of fresh water by biological method, and also in 1983 by China's Shen. Since its introduction, it has been widely used in biology and environmental studies to investigate aquatic ecosystems and to assess the degree of contamination in fresh water. And, compared with the collection method for other micro-animal community analysis, the collection method for micro-animal community analysis using the polyurethane foam as described above is a systematic, practical, and functional for bio-assessment It is evaluated as an enemy.

However, while the microbial collection method using the porous foam as described above has been widely used to analyze the degree of contamination of fresh water, there is a problem that cannot be used in seawater. The inventors of the present invention attempted to apply a microbial collection method using porous foam to seawater in coastal waters for the first time in 1999, but due to continuous circulation of seawater such as tidal current or nontidal current which is not present in freshwater The problem was caught. That is, the collecting means including the polyurethane foam which is located in the water for collecting is severely fluctuated due to the influence of the flow, and becomes smaller due to abrasion while directly opposed to the flow for a long time to achieve the desired collecting effect. There was a problem difficult to do. In addition, when considering a work environment that must always consider a certain amount of waves, there was a problem that the worker is difficult to handle easily.

In order to solve the problems of the prior art as described above,

An object of the present invention is to prevent the miniaturization due to abrasion of the embedded porous foam even in the state exposed to extreme flow, and easy to handle even in a harsh working environment to collect the microbial microorganisms to enable efficient collection of microorganisms To provide.

1 is a perspective view showing an embodiment according to the present invention,

2 is a cross-sectional view of FIG.

3 is a bottom view of FIG. 1,

4 is a plan view of FIG.

5 is a perspective view of a collecting container according to an embodiment of the present invention.

* Description of the symbols for the main parts of the drawings *

5: bottom plate 10: bottom frame

15: top plate 17: access hole

20: upper frame 25: the middle array plate

27: fixing hole 30: intermediate frame

35: connecting portion 40: center support shaft

41, 42, 43, 44: spacer 45: bearing bracket

50: roller bearing 60: shock absorbing member

65, 66, 67: mounting bracket 70: fixing means

80: butterfly bolt 100: collecting container

110: slot

The present invention for realizing this,

A plurality of collecting containers each having an open top and having a plurality of axial slots at an outer circumference thereof and into which a porous foam is introduced;

A center support shaft having connecting portions at both ends;

A lower frame disposed below the central support shaft and coupled to form a lower plate on which the plurality of collecting containers are raised in an upright state and supporting a bottom of the lower plate and surrounding an edge thereof;

An upper frame disposed on an upper portion of the central axis so as to have a distance corresponding to a height of the lower plate and the collection container, and an upper frame disposed in a shape surrounding a center edge allowing rotation of the upper plate and at least one entrance hole; ;

An intermediate array plate disposed between the lower plate and the upper plate and having a plurality of fixing holes formed according to a predetermined shape and an arrangement rule for fixing the collection container one by one, and surrounding the edges of the intermediate array plate; Provides an aquatic microbial harvesting device comprising a; an intermediate frame coupled to.

And two spacers inserted into the central support shaft so as to be positioned between the lower plate and the intermediate array plate and between the upper plate and the intermediate array plate, respectively, to maintain a constant gap between the plate and the plate.

The lower frame, the upper frame, and the intermediate frame each have a plurality of coupling brackets protruding in a radial direction at positions corresponding to each other. It characterized in that it comprises a plurality of fixing means for connecting the coupling brackets aligned in the state matched by the reference so that a constant interval can be maintained.

Here, the upper frame is provided with a plurality of bearing brackets extending from the outer circumferential surface to the bottom, and a plurality of bearing members installed on the center side of each of the bearing brackets, the movement of the upper plate in the axial direction Preventing and rotating is constrained to be possible.

On the other hand, the plurality of fixing holes of the intermediate array plate is formed with a diameter corresponding to the outer diameter of the collection container, respectively, and arranged so that each center is located on one or more orbits forming a concentric circle with respect to the center support axis It features.

In addition, the entrance hole of the upper plate is the respective ruling and center distance of the middle array plate so that the position coincides at least once with each of the fixing holes provided in the intermediate array plate during the rotation of the upper plate relative to the center support axis It is characterized in that each one provided on the same concentric circle with.

And it is characterized in that it comprises a rotation preventing member for selectively restraining the rotation of the upper plate by providing a pressing force toward the bearing member in at least one position of the upper frame where the bearing member is located.

Finally, it characterized in that it comprises a plurality of shock absorbing member is fixed to the outer peripheral side between the lower frame and the lower frame.

BEST MODE Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the accompanying drawings.

1 is a perspective view showing an embodiment according to the present invention, Figure 2 is a cross-sectional view of Figure 1, Figure 3 is a bottom view of Figure 1, Figure 4 is a plan view of Figure 1, Figure 5 of the present invention A perspective view of a collecting container according to an embodiment.

The aquatic microbial collecting device according to a preferred embodiment of the present invention is largely arranged in a plurality of collecting containers 100, one central support shaft 40 and the central support shaft 40 in the upper and lower sides of the plurality of collecting containers ( 100) consists of three plates that are fixed to restrain movement in each of the up and down directions in a constant arrangement.

As shown in FIG. 5, the collecting container is positioned between the lower plate and the upper plate, with the upper portion being open and having a plurality of axial slots 110 at the outer circumference thereof and having a porous foam injected therein. The porous foam may be used in many kinds, but it is preferable to use a polyurethane foam which is commonly available in the surroundings and suitable as a habitat for microorganisms. The slot 110 is intended to allow the access of water into and out of the collection container in water. If too large, the effect of algae or current is directly transmitted to the polyurethane foam. It is impossible to control the microorganisms in a suitable size in consideration of the difficulty of clustering, and the number is appropriately selected in consideration of the efficient aspect.

The central support shaft 40 serves as a rotating shaft as well as a skeleton supporting the device structurally, as shown in Figure 2, the upper and lower ends are provided with connection portions 35, respectively. The connection part 35 may be provided integrally or separately in the central support shaft 40, and may be provided in an annular shape so as to connect a connection means such as a hook or a shackle.

In the connection part located at the bottom of the central support shaft, a rope with a position fixing means such as an anchor for fixing the position of the device to the sea bottom is connected through a connection means such as a shackle, and the upper connection part facilitates the position identification and lifting work. A rope suspended from a buoy is also connected through a connecting means such as a shackle.

The lower plate 5 is to raise the collecting container 100 in the standing state, it is provided as a circular plate of transparent plastic material. And, the lower plate 5 is provided with a lower frame 10 to perform the structural support while located under the self. The lower frame 10 is also coupled in a form surrounding the edge of the lower plate, and is firmly coupled to each other using a coupling member such as a screw or a bolt. In addition, the lower plate and the lower frame are positioned below the central support shaft 40 in a coupled state.

The upper plate 15 is disposed on the upper portion of the central support shaft 40 to have a distance corresponding to the height of the lower plate 5 and the collection container 100, and is provided with the same diameter and material as the lower plate, one The above entrance hole 17 is provided. The access hole 17 is for inserting and removing the collection container 100 is fixed between the lower plate and the upper plate, three embodiments of the access hole 17 is provided. In addition, the upper plate 15 is provided with the upper frame 20 positioned in the form of surrounding the edge allowing the rotation thereof, and is not directly coupled to each other. The fixing of the upper frame 20 will be described in more detail below.

The intermediate array plate 25 is disposed on the intermediate support shaft 40 so as to be located between the upper plate and the lower plate, and is provided with the same diameter and material as the lower plate 5 and surrounds the edge thereof. The intermediate frame 30 is provided together. The intermediate array plate 25 includes a plurality of fixing holes 27 formed according to a predetermined shape and an arrangement rule for fixing the collection container 100 one by one.

And a spacer 43 fitted between the lower plate 5 and the intermediate array plate 25 of the central support shaft 40 and between the upper plate 15 and the intermediate array plate 25, respectively. 44) to keep the plate and plate constant. In addition, spacers 41 and 42 are provided at the bottom and top of the center support shaft 40, that is, just above the top plate and just below the bottom plate, respectively to limit the movement in the axial direction. Suppresses movement

The lower frame 10, the upper frame 20, and the intermediate frame 30 each have four joining brackets 65, 66, 67 protruding in a radial direction from corresponding positions. do. Coupling brackets 65, 66, 67 formed in each of the frames 10, 20, 30 can be maintained at regular intervals in a state arranged in a straight line aligned with respect to the center support shaft (40). So that it is coupled by the fixing means 70. That is, it is fixed by the fixing means 70 at each of the four points. In the present embodiment, a fixing means 70 consisting of one long bolt and six nuts is used, and the nuts are positioned below and above each of the brackets 65, 65 and 67, respectively, to maintain the spacing.

In addition, the upper frame 20 has four bearing brackets 45 extending downward from the outer circumferential surface, each of the bearing brackets 45 is provided with one roller bearing 50 installed at the center side thereof. do. The upper plate 15 is located between the upper frame 20 and the roller bearing 45, the movement in the axial direction is suppressed and the rotation is constrained in a possible state. In addition, the roller bearing 50 serves to facilitate the rotation of the top plate.

On the other hand, the plurality of fixing holes (27) provided in the intermediate array plate 25 is formed with a diameter corresponding to the outer diameter of the collection container 100, respectively, as shown in Figures 1, 3 and 4 Likewise, the centers of the fixing holes are arranged on three orbits forming a concentric circle with respect to the center support shaft 40.

In addition, the entrance and exit holes 17 of the upper plate 15 are fixed to each of the fixing holes 27 provided in the intermediate array plate 25 during the rotation of the upper plate 15 with respect to the center support shaft 40. 1 and 4 so that the positions coincide with each other, as shown in FIG. In addition, the shape is basically the same circular shape and the same diameter as the fixing hole 27, but in order to facilitate the input and discharge of the collection container 100 made by hand, the two opposite parts of the collection container is introduced It is expanded to facilitate gripping in the closed state.

In addition, an anti-rotation butterfly bolt 80 is provided on the upper frame 20 corresponding to one of the rolling bearings 50. The butterfly bolt 80 is mounted on the rolling bearing 50 at the time of forward rotation. Providing the pressing force to the top plate 15 is in close contact with the surface of the rolling bearing 50 so that the rotation is impossible, the reverse rotation serves to enable the rotation of the top plate (5).

1, 3, and 4, four shock absorbing members 60 are provided on the outer peripheral surface side of the apparatus. This is to prevent the damage of the device due to the collision, considering that the device of the present invention is mainly used in the coastal waters where the traffic of the ship is frequent, the present embodiment is intended to prevent the impact during a collision in a general small ship The shock absorbing member used as was used. This shock absorbing member also serves to balance the device by its buoyancy.

Referring to the method of using the aquatic microbial collecting device according to the present invention as described above is as follows.

First, the upper plate 15 is rotated little by little, and each time the entrance hole 17 and the fixing hole 27 coincide with each other, the collecting container 100 containing the polyurethane foam inside each fixing hole 27 is opened. Put one by one.

Then, the upper plate 15 is rotated so that the entrance hole 17 of the upper plate 15 and the fixing hole 27 of the intermediate array plate 25 are not in the same position, and then the butterfly bolt 80 Tighten the top plate 15 so that it is impossible to rotate. That is, in any situation, the collection container 100 is to be kept in a state that can not be separated to the outside.

When the input of the collecting container 100 is completed in consideration of the depth of the water in which the device is installed, the anchoring rope and the buoy to adjust the length of the rope, then the device according to the invention is put into the sea.

Then, after a predetermined time, the device is pulled up to the surface of the water, the butterfly bolt 80 is released, the upper plate 15 is rotated, and the collecting container is removed while matching the positions of the entrance hole and the fixing hole, and then the collecting container. A series of gatherings are performed by squeezing the polyurethane foam and placing the contents in a sample container.

Such a collection operation using the collection device of the present invention, if it is to be repeatedly performed several times with a time difference in order to analyze the change and trend of the microbial community, the collection container discharge operation is carried out all the collection containers ( Rather than taking out all the 100), it proceeds by dividing several times at a time by a certain number of times. For example, take out a certain number of collecting containers 100 at a time, put the device in the water again, and after a certain time, the device is pulled out of the water again, and a certain number of collecting containers 100 again It's going to go out.

On the other hand, if the target period is very long, it may be used by putting a new collection container back into the empty fixing hole every time the collection container is taken out.

The aquatic microbial collecting device according to the present invention as described above reduces the impact of algae and currents directly on the porous foam such as polyurethane foam used for microbial collection to minimize the damage and during the collection operation. It is easy to handle so that it is possible to collect microorganisms stably and effectively, and furthermore, it has an effect of improving the reliability of the results for the assessment of the pollution degree and the health of the marine ecosystem by analyzing the microorganisms.

While the invention has been shown and described in connection with specific embodiments, it is conventional in the art that various design changes and variations are possible without departing from the spirit and scope of the invention as indicated by the claims. Anyone with knowledge will know it easily.

Claims (8)

A plurality of collecting containers each having an open top and having a plurality of axial slots at an outer circumference thereof and into which a porous foam is introduced; A center support shaft having connecting portions at both ends; A lower frame disposed below the central support shaft and coupled to form a lower plate on which the plurality of collecting containers are raised in an upright state and supporting a bottom of the lower plate and surrounding an edge thereof; An upper frame disposed on an upper portion of the central axis so as to have a distance corresponding to a height of the lower plate and the collection container, and an upper frame disposed in a shape surrounding a center edge allowing rotation of the upper plate and at least one entrance hole; ; An intermediate array plate disposed between the lower plate and the upper plate and having a plurality of fixing holes formed according to a predetermined shape and an arrangement rule for fixing the collection container one by one, and surrounding the edges of the intermediate array plate; An aquatic microbial harvesting device comprising a; intermediate frame coupled to. The method of claim 1, An aquatic microorganism comprising two spacers inserted into the central support shaft so as to be positioned between the lower plate and the intermediate array plate and between the upper plate and the intermediate array plate, respectively, to maintain a constant gap between the plate and the plate. Picking device. The method according to claim 1 or 2, The lower frame, the upper frame, and the intermediate frame each have a plurality of coupling brackets protruding in a radial direction at positions corresponding to each other, and the positions of the coupling brackets formed on the respective frames with respect to the center support shaft. An aquatic microbial collecting device, characterized in that it comprises a plurality of fixing means for connecting the coupling brackets aligned in a matched state to maintain a constant interval. The method of claim 3, wherein The upper frame has a plurality of bearing brackets extending from the outer circumferential surface to the lower portion, and includes a plurality of bearing members installed at the center of each bearing bracket to prevent movement of the upper plate in the axial direction. Aquatic microbial collecting device, characterized in that the rotation is constrained to be possible. The method of claim 4, wherein And a rotation preventing member for selectively restraining rotation of the upper plate by providing a pressing force toward the bearing member at at least one position of the upper frame in which the bearing member is located. The method according to claim 1 or 5, The plurality of fixing holes of the intermediate array plate is formed with a diameter corresponding to the outer diameter of the collecting container, respectively, characterized in that arranged in such a way that each center is located on one or more orbits forming a concentric circle with respect to the center support axis. Aquatic microbial collecting device. The method of claim 6, The entrance and exit holes of the upper plate are the same as the respective trajectories and center distances of the middle array plate such that their positions coincide with each of the fixing holes provided in the intermediate array plate at least once during rotation of the upper plate about the central support shaft. Aquatic microbial collecting device, characterized in that each one provided on the concentric circles. The method of claim 1, And a plurality of shock absorbing members fixed to an outer circumferential side between the lower frame and the lower frame.