KR100439426B1 - Composition for anti-aging cosmetics contain zooplankton extract - Google Patents

Abstract

The present invention relates to an anti-wrinkle cosmetic composition containing an zooplankton extract, and more particularly, to an extract obtained from animal plankton artemia salina ( Artemia salina ) and a cosmetic composition having an anti-wrinkle effect containing the extract. .

Description

[Composition for anti-aging cosmetics contain zooplankton extract}

The present invention relates to an anti-wrinkle cosmetic composition containing an zooplankton extract, and more particularly, to an extract obtained from animal plankton artemia salina ( Artemia salina ) and a cosmetic composition having an anti-wrinkle effect containing the extract. .

The skin is composed of epidermis, dermis and subcutaneous fat. Among them, collagen fiber, which is a major component in dermis, because quantitative or qualitative change of components in dermis acts as an important factor for physical and biochemical changes of skin tissue. Observing changes in interfibrous materials such as elastic fibers and point polysaccharides, electrolytes, and moisture is very important for understanding the aging process of the skin.

Skin aging is largely classified into aging according to human life and photoaging caused by ultraviolet light. In some cases, it is difficult to distinguish the two processes strictly, and in the part of the body that is chronically exposed to sunlight, the skin proceeds simultaneously. Understanding aging requires a comprehensive approach to these processes.

Changes in skin tissue with age include increased wrinkles, decreased skin thickness (epidermis and dermis), degeneration of skin appendages, changes in constituents in the dermis, and the like.

In particular, many substances in the dermis have been studied in relation to skin aging, and they are composed of macromolecules such as proteoglycan, which is an amorphous substance and proteoglycan in which the polysaccharide is bound to proteins. It is believed to exist in a semi-solid state to support various cells and skin structures in the dermis.

Known anti-wrinkle agents are moisturizers that improve the wrinkles by recovering the abnormality of keratin accompanying dry skin, increase the collagen biosynthesis, improve the elasticity of the skin, improve the wrinkles, and have the antioxidant effect And substances that inhibit transdermal moisture evaporation.

Examples of the moisturizer having an anti-wrinkle effect include hyaluronic acid or sodium chondroitin sulfate, and retinoid acid, which is widely used in medicines, is also known to have excellent anti-wrinkle effect.

However, when using retinoid acid, the skin becomes red, scales occur, and side effects such as a sensitive reaction caused by ultraviolet light have been reported, and there is a problem in that the effect appears only after 6 months of continuous use. In addition, in view of the anti-wrinkle effect, alpha hydroxy acid, which has a similar effect to retinoid acid, is known as an anti-wrinkle agent, but in order to have an effect of preventing or improving wrinkles, a large amount (1.0 to 10.0%) must be used, thus itching, erythema, and skin abnormalities It is accompanied by side effects such as skin irritation such as keratin phenomenon can not satisfy the safety aspects of the product pursued cosmetics has a problem that can not only deteriorate the value as cosmetics.

Accordingly, the present inventors have studied to provide a cosmetic having a more effective anti-wrinkle action without irritation to the skin, and as a result, keratinocytes of the skin, fibroblasts, and regeneration of the skin, which are the main causes of skin aging, can inhibit collagen biosynthesis amount of reduction in effective and were retrieving marine material that is effective in the protection and regeneration of the cell affected by the culprits of ultraviolet light of photoaging wherein Arte mia Salina excellent effect zooplankton extract of (Artemia salina) By knowing that the present invention was completed.

In addition, Artemia Salina was found to vary greatly in the amount of active ingredients depending on water and salt (salt), which are important factors for the growth and growth of zooplankton.

Accordingly, an object of the present invention is to provide an extract obtained from zooplankton called Artemia salina and a cosmetic composition for preventing wrinkles containing the extract.

In the anti-wrinkle cosmetic composition, an anti-wrinkle cosmetic composition containing Artemia salina, which is an zooplankton, is contained in an amount of 0.01 to 50.0 wt% based on the total weight of the cosmetic composition.

In addition, the Artemia salina extract is characterized by anti-wrinkle cosmetic composition obtained by hydrothermal extraction by adding salt, preferably general salt or high purity salt of the larva of Artemia salina.

The present invention will be described in more detail as follows.

Artemia Salina is a larvae of crustaceans that live in seawater, also called Brine Shrimp, which is as small as 2 mm in size and has a unique biological adaptation mechanism that allows it to survive special growth processes and saturated salts. . This zooplankton is often used as a biochemical and molecular research model for differentiation for this reason.

Artemia Salina becomes a zooplankton encased in a cyst and survives harsh environments such as complete dehydration. At this time Artemia Salina enters a state of hibernation, which can withstand long-term dryness and adverse conditions. And when seawater is supplied again, growth resumes. Salt and temperature are reported to be a physiological signal that resumes some metabolism.

The extraction method of Artemia salina extract of the present invention is as follows.

First, the commercially available is dried eggs of Artemia salina, which can be easily hatched and used in the laboratory. Incubate 1 liter of water in two beakers, add 4g of normal salt and 4g of high-purity salt called "Energy Flakes Salt" developed by our company, and add 2g of dried Artemia salina eggs. If it is aerated at 30 ℃, it will hatch after 24 hours. If the aeration is too weak, the eggs do not circulate in water, so the hatching rate drops. If too large, the hatched eggs die quickly, so it is desirable to maintain the minimum strength that the whole egg can circulate.

Three times of water was added to the solution containing the hatched Artemia salina, followed by extraction of hot water at 100 ° C. for 3 hours, followed by filtration twice. This filtrate is filtered using an ultrafiltration membrane having a molecular weight of 10,000. The filtered solution was put in a dry oven and dried, and the composition of the dried product was analyzed, and the analysis results are shown in Table 1 below.

As a result of Table 1, the dry matter composition ratio of the filtrate hatched by the addition of general salt and the filtrate hatched by the addition of high purity salt is the same. Here the composition of the nucleotide was analyzed in detail by HPLC and TLC. The analysis results are shown in Table 2 below.

The extract obtained by adding high-purity salt and incubating the same as the extract obtained by incubating with normal salt and hatching is 2%, but the composition of diguanosine tetraphosphate is larger.

Hereinafter, the present invention will be described in detail based on Examples, but the present invention is not limited to Examples.

Test Example 1 Measurement of Human Fibroblast Proliferation Effect

Human fibroblasts cultured in Dulbecco's Modified Eagle's Media (DMEM) medium containing 3.5% fetal bovine serum were dispensed in a 96-well microtiter plate at 5,000 cells / well and each Artemia salina extract was extracted. After diluting sequentially with 1 × 10 to 1 × 10 ^-3 % by weight as a culture medium and incubated for 4 days at 37 ℃. After incubation, 50 μl of 0.2% MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium bromide) solution was added to each well, followed by incubation for 4 hours at 37 ° C. Formazan was dissolved in DMSO (Dimethyl sulfoxide). The absorbance of the dissolved formazan was measured at 570 nm using a microplate reactor. The control group not treated with Artemia salina extract was carried out in the same manner as above to measure the absorbance. After comparing the absorbance of the experimental group and the control group containing Artemia salina extract, the results are shown in Table 4 below. At this time, general salt was used commercially available edible salt. For high-purity salt, the general salt was re-crystallized 10 times or more from the company to remove all impurities contained in the general salt, and to the water containing a large amount of minerals. An energy flake salt developed by melting and recrystallizing was used.

As can be seen in Table 3, both showed a high fibroblast proliferation effect, the extract obtained by the addition of the energy flake salt hatching than the extract obtained by the addition of the normal salt showed a better proliferation effect.

Test Example 2 Measurement of Keratinocyte Proliferation Effect

Using keratinocytes, proliferative efficacy of keratinocytes was measured in the same manner as in Test Example 1, and the results are shown in Table 4 below.

As can be seen in Table 4, both showed keratinocyte proliferative effect, and the extract obtained by incubation by adding energy flake salt showed higher proliferative effect.

Test Example 3 Measurement of Collagen Cell Biosynthesis Proliferation Effect

After culturing human fibroblasts in a 24-well plate incubator, each Artemia salina extract was added by diluting sequentially from 1 × 10 to 1 × 10 ⁻³ wt% with culture medium. On day 3 of culture, 0.5 ml of DMEM medium containing 10% fetal bovine serum was added to each well, followed by 10 ci of L [2,3,4,53H] -proline. After 24 hours, the medium and cells in each well were collected, washed with 5% trichloroacetic acid (TCA) solution, washed in two test tubes, and one unit of type I collagenase (unit I collagenase). Μl was added and incubated at 37 ° C. for 90 minutes, and other test tubes were stored at 4 ° C. Subsequently, 0.05 ml of 50% TCA was added to all test tubes and allowed to stand at 4 ° C. for 20 minutes, followed by centrifugation at 15,000 rpm for 10 minutes, respectively. By obtaining the decay per minute value, the collagen biosynthesis values of the experimental and non-treated groups treated with each Artemia salina extract were obtained, and the results are shown in Table 5 below.

As can be seen in Table 5, both showed collagen biosynthesis promoting effect, the extract obtained by incubation by the addition of energy flake salt showed higher collagen biosynthesis promoting effect.

In the above test example, it can be seen that the Artena salina extract is a substance that promotes skin cell proliferation and collagen biosynthesis. In particular, the extract obtained by adding high-purity salt shows a higher effect.

Example 1, 2 and Comparative Example 1

60 test subjects with facial wrinkles over 30 years of age were compared with the essence having the composition shown in Table 6 below to compare the skin wrinkle improvement effects of Examples 1 and 2 and Comparative Example 1 containing no Artemia salina extract, Evaluated.

The test subjects were divided into three groups of 20 people each to use Examples 1, 2 and Comparative Example 1, and before using, the skin condition was measured on both sides of the face. The change in skin wrinkles was measured.

Skin measurement was carried out in a constant temperature and humidity room of 24 ℃, relative humidity 40%, the wrinkles of the tail of the eye area was replicated (replica) to measure the skin wrinkles (Visiometer system; C + K company). The replica is analyzed three-dimensionally with a Charge Coupled Device (CCD) camera, and the analysis of skin wrinkles shows how the wrinkle height decreased after 3 months of use from the height of wrinkles measured before using essence. Each average was shown in Table 7 below.

As a result of the experiment, the skin wrinkle height was decreased from 0.038 to 0.078 mm (mean value: 0.05 mm) in Comparative Example 1 and 0.121 to 0.153 mm (mean value: 0.139 mm) in Example 1, and the wrinkles were statistically significant range. It can be seen that it was definitely improved (P <0.01), and in Example 2, it was lowered by 0.133 to 0.163 mm (average value: 0.145 mm), and it can be seen that it was certainly improved within a statistically significant range (P <0.01). . Comparing Example 1 and Example 2, it can be seen that Example 2 has a slightly higher wrinkle improvement effect than Example 1.

The anti-wrinkle cosmetic composition of the present invention is not particularly limited in the formulation, for example, may be formulated into a cosmetic such as softening longevity, nourishing longevity, nutrition cream, essence, eye cream, eye essence, pack, cleansing foam. . Examples that can be formulated are shown in Table 8 below.

(continue)

As described above, the anti-wrinkle cosmetic composition of the present invention is an extract obtained by adding Artemia salina , an animal plankton, to salt, preferably general salt or high-purity salt, and contains a large amount of protein and nucleotide.

Fibroblast proliferation efficiency in Test Example 1, keratinocyte growth proliferation effect in Test Example 2 and collagen biosynthesis promoting effect in Test Example 3 showed excellent effects in both extracts added with normal salt and extracts added with energy flake salt, In particular, extracts with added energy flake salt showed higher potency levels. As a result of evaluating the improvement of wrinkles in the tail of the human eye in Test Example 4, both the normal salt and the energy flake salt addition extract showed a clear wrinkle improvement effect, and the energy flake salt addition extract showed a higher improvement effect. It was.

Therefore, it can be widely applied effectively in various cosmetic fields that can improve skin wrinkles by containing Artemia salina extract.

Claims (4)

The active ingredient is an anti-wrinkle cosmetic composition, characterized in that 0.01 to 50.0% by weight of the extract obtained by hydrothermal extraction of Artemia salina (animal plankton) with salt. delete The anti-wrinkle cosmetic composition according to claim 1, wherein the salt is a general salt or a high purity salt. According to claim 1, wherein the cosmetic cosmetics for preventing wrinkles, characterized in that the soft cosmetics, nourishing cosmetics, nutrition cream, essence, eye cream, eye essence, pack or a formulation of a cleansing foam.