KR100421464B1 - Manufacturing Method of Absortive Copper-Oligopeptide in The Human Body - Google Patents

Manufacturing Method of Absortive Copper-Oligopeptide in The Human Body Download PDF

Info

Publication number
KR100421464B1
KR100421464B1 KR10-2000-0039593A KR20000039593A KR100421464B1 KR 100421464 B1 KR100421464 B1 KR 100421464B1 KR 20000039593 A KR20000039593 A KR 20000039593A KR 100421464 B1 KR100421464 B1 KR 100421464B1
Authority
KR
South Korea
Prior art keywords
copper
oligopeptide
oligopeptides
protease
protein
Prior art date
Application number
KR10-2000-0039593A
Other languages
Korean (ko)
Other versions
KR20020006112A (en
Inventor
지성규
Original Assignee
지성규
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 지성규 filed Critical 지성규
Priority to KR10-2000-0039593A priority Critical patent/KR100421464B1/en
Publication of KR20020006112A publication Critical patent/KR20020006112A/en
Application granted granted Critical
Publication of KR100421464B1 publication Critical patent/KR100421464B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23001—Pepsin A (3.4.23.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23002—Pepsin B (3.4.23.2)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/66—Proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicinal Preparation (AREA)

Abstract

본 발명은 체내에서 흡수되기 용이한 구리-올리고펩티드의 제조방법에 관한 것이다. 보다 상세하게는 식물성 또는 동물성 단백질을 물에 현탁시키고 단백분해효소를 첨가하여 pH 3.5∼6.0에서 12시간 반응시켜 올리고펩티드(Oligopeptide)를 얻은 후, 단백분해물인 올리고펩티드에 구리미네랄 이온을 첨가하여 킬레이트(Chelate) 반응에 의하여 구리-올리고펩티드(Zinc-Oligopeptide)가 얻어진다. 이를 고형분이 35% 정도 되도록 농축한 다음 건조하여 분말화하면 분말 구리-올리고펩티드를 얻는다.The present invention relates to a method for preparing copper-oligopeptides which is easily absorbed in the body. More specifically, vegetable or animal proteins are suspended in water, and protease is added to react for 12 hours at pH 3.5 to 6.0 to obtain oligopeptides, followed by chelates by adding copper mineral ions to the protease oligopeptides. By the (Chelate) reaction, a copper-oligopeptide (Zinc-Oligopeptide) is obtained. It is concentrated to about 35% solids, dried and powdered to obtain powdered copper-oligopeptide.

본 발명의 구리-올리고펩티드를 이용하여 체내에서 흡수되기 용이한 구리-올리고펩티드 음료 또는 식품 조성물로 하여 구리결핍을 해결하는 데 기여할 수 있다.By using the copper-oligopeptide of the present invention as a copper-oligopeptide beverage or food composition that can be easily absorbed in the body can contribute to solving the copper deficiency.

Description

체내에 흡수되기 용이한 구리-올리고펩티드의 제조방법{Manufacturing Method of Absortive Copper-Oligopeptide in The Human Body}Manufacturing Method of Absortive Copper-Oligopeptide in The Human Body

본 발명은 체내 흡수력이 강하고 식품성분 중 장해요인에 영향을 받지 않는 구리-올리고펩티드(Copper-Oligopeptide)의 제조방법 및 이를 함유한 음료 또는 식품 조성물에 관한 것이다. 보다 상세하게는 지금까지 사용되고 있는 미네랄강화물질들은 모두가 체내흡수가 불가능한 것들뿐이고 소화관내 소장에서 흡수될 수 있는 미네랄화합물은 인체 내에서 유통되고 이용되고 있는 칼슘화합물인 칼모듀린(Calmodulin)과 같은 것이라는 것이 밝혀졌고 칼모듀린의 구조가 칼슘이온을 킬레이트하고 있는 올리고펩티드에 의존하고 있음을 발견하여 구리-올리고펩티드의 제조방법을 발명하게 된 것이다.The present invention relates to a method for preparing copper-Oligopeptide and a beverage or food composition containing the same, which have strong absorption in the body and are not affected by obstacles among food ingredients. More specifically, all the mineral-enhancing substances used so far are those that cannot be absorbed by the body, and mineral compounds that can be absorbed by the small intestine in the digestive tract are the same as calmodulin, a calcium compound that is distributed and used in the human body. It was found that the structure of calmodurin depends on the oligopeptide chelates calcium ions, thereby inventing a method for preparing copper-oligopeptide.

현대인의 가공식품 섭취 과다로 인한 미네랄 흡수저해물질의 증가와 인체의 노화에 의한 흡수능력의 저하는 체액이 보유해야 할 미네랄의 밸런스 상실로 성인병을 증가시킨다. 그리고 미네랄 부족으로 체내에서 진행되는 생리작용의 왜곡은 암이라는 이상변이물질의 발생을 부추기고 있다. 그 중에서도 구리의 부족으로 인하여 발생되는 성인병은 어른들뿐만 아니라 어린이들에게까지 확산되고 있는 것이 현실이다.Increasing mineral absorption inhibitors due to excessive consumption of processed foods and deterioration of absorption ability due to aging of the human body increases adult disease due to loss of balance of minerals to be retained by body fluids. In addition, the distortion of physiological processes in the body due to the lack of minerals is promoting the occurrence of abnormal variants called cancer. Among them, the adult disease caused by the lack of copper is spreading to children as well as adults.

구리의 생리활성은 동양의학에서 조혈(調血)과 정력(精力)에 관여하는 것으로 알려지고 있다. 조혈이라 함은 혈액을 다스린다는 뜻으로 허혈성질환에 해당될 뿐만 아니라 정력을 유지하는데 중요한 역할을 한다. 체내에서 혈액의 조절이 순조롭지 못하면 체외로부터의 산소공급이 불순하여 에너지생산이 순조롭지 못하게 된다. 구리는 철과 함께 조혈에 영향을 준다. 구리의 기능은 조혈(Hemoglobin format ion), 머리카락의 구조와 색소 조절(Pigmentation and structure of hair), 심장장해회복(Reproduction cardiovascular lesions), 퇴행성신경질환의 방어(Protect de generation of the nervous system.), 면역기능의 향상(Enhances the immune systems) 등을 발휘한다.The physiological activity of copper is known to be involved in hematopoiesis and energy in oriental medicine. Hematopoietic means ruling the blood and is not only an ischemic disease but also plays an important role in maintaining energy. If the blood is not well regulated in the body, the oxygen supply from the outside is impure and energy production is not smooth. Copper, along with iron, affects hematopoiesis. Copper functions include hemoglobin format ion, Pigmentation and structure of hair, Reproduction cardiovascular lesions, Protect de generation of the nervous system, Enhances the immune systems.

본 발명은 수용성단백질인 올리고펩타이드가 구리미네랄을 킬레이트하고 있는 형태로서 체내에서 흡수가 잘 되는 구리-올리고펩티드를 제공하는 것을 목적으로 한다.An object of the present invention is to provide a copper-oligopeptide which is a water-soluble protein in a form in which the oligopeptide chelates copper minerals and is well absorbed in the body.

도 1은 흡수성 구리-올리고펩티드의 구조를 나타낸 것이다1 shows the structure of absorbent copper-oligopeptides

도 2는 올리고펩타이드를 나타낸 것이다.2 shows an oligopeptide.

본 발명의 구리-올리고펩티드는 단백질을 정제수에 현탁시키고 단백분해효소를 첨가하여 약산성 영역에서 장시간 반응시켜 올리고펩티드를 얻는 단계와, 단백분해물인 올리고펩티드에 대하여 구리미네랄 이온을 첨가하여 킬레이트 반응시켜 구리-올리고펩티드를 제조한다. 본 발명의 구리-올리고펩티드는 도 2와 같은 올리고펩티드에 구리이온을 킬레이트 반응시켜 도 1과 같이 올리고펩티드 안에 구리를 감싸 안은 형태를 의미한다.The copper-oligopeptide of the present invention is suspended in purified water and the proteolytic enzyme is added to react for a long time in a weakly acidic region to obtain oligopeptides. Prepare oligopeptides. The copper-oligopeptide of the present invention refers to a form in which copper is wrapped in an oligopeptide as shown in FIG. 1 by chelation of copper ions with an oligopeptide as shown in FIG. 2.

본 발명의 구리-올리고펩티드는 단백질 100%를 정제수 800%에 현탁시키고 단백분해효소를 2∼4% 첨가하여 pH 3.5∼6.0에서 11∼12시간 반응시켜 올리고펩티드를 얻은 후, 올리고펩티드 1,000부에 대하여 구리미네랄 이온 한 원자를 첨가하여 킬레이트 반응시켜 구리-올리고펩티드를 제조한다. 한편 전기의 구리-올리고펩티드를 고형분이 32∼36% 범위가 되도록 농축한 후 건조하여 분말화된 구리-올리고펩티드를 제조할 수 있다.The copper-oligopeptide of the present invention was suspended in 800% purified water and added 2-4% of protease and then reacted at pH 3.5-6.0 for 11-12 hours to obtain oligopeptide, followed by 1,000 parts of oligopeptide. The copper-oligopeptide is prepared by chelate reaction with the addition of one atom of copper mineral ion. Meanwhile, the aforementioned copper-oligopeptide may be concentrated to a solid content in the range of 32 to 36% and dried to prepare a powdered copper-oligopeptide.

상기에서 단백질은 식물성 또는 동물성 중에서 선택된 어느 하나이고, 단백분해효소는 펩신 또는 트립신 중에서 선택된 어느 하나이거나 둘 이상을 함유하는 것을 사용한다.The protein is any one selected from vegetable or animal, and the protease is used any one selected from pepsin or trypsin or containing two or more.

한편 상기에서 얻은 구리-올리고펩티드를 유효성분으로 하고 비타민-C, 비타민-B1, 비타민-B2, 과당, 정제수와 같은 공지의 음료조성물을 포함하여 구리-올리고펩티드가 체내에 흡수되기 용이하도록 구리-올리고펩티드 음료를 제조할 수 있으며 조성물중의 수분을 제거하고 콜라겐을 포함하여 캡슐 또는 정제 형태의 구리-올리고펩티드를 제조할 수 있다.Meanwhile, the copper-oligopeptides obtained above are used as an active ingredient and known beverage compositions such as vitamin-C, vitamin-B 1 , vitamin-B 2 , fructose and purified water are used to facilitate the absorption of copper-oligopeptides into the body. Copper-oligopeptide beverages can be prepared and copper-oligopeptides in capsule or tablet form can be prepared by removing moisture in the composition and including collagen.

구리-올리고펩티드 음료에 있어서 유효성분인 구리-올리고펩티드는 1∼50% 포함할 수 있다.Copper-oligopeptide The active ingredient copper-oligopeptide in the beverage may contain 1 to 50%.

이하 본 발명을 다음의 실시예에 의하여 설명하고자 하나 이들이 본 발명의 기술적 범위를 한정하는 것은 아니다.Hereinafter, the present invention will be described by the following examples, but these are not intended to limit the technical scope of the present invention.

＜실시예 1＞<Example 1>

단백질은 식물성 단백질인 콩단백질과 글루텐, 동물성 단백질인 카제인과 젤라틴을 이용하는데 이들 단백질 중에서 한가지를 선택하여 100%에 물 800%에 현탁시키고 단백분해효소로 프로테아제 3%를 첨가하여 pH 3.5∼6.0에서 12시간 반응시켜 올리고펩티드를 얻었다. 이때 단백질 분해물 중 비수용성 아미노산은 분리되므로 여과하여 제거하였다. 다음 단백분해물인 올리고펩티드 1,000부에 대하여 구리미네랄 이온 한 원자량을 계산하여 첨가한 후 반응시켜 구리-올리고펩티드를 제조하였다. 이를 고형분이 35% 정도 되도록 농축한 다음 드럼건조기나 분무건조기로 분말화하여 분말 구리-올리고펩티드를 얻었다.Protein is derived from soy protein, gluten and animal protein casein and gelatin. One of these proteins is selected and suspended in 100% in 800% water and 3% in protease with protease. The reaction was carried out for 12 hours to obtain an oligopeptide. At this time, the water-insoluble amino acid in the protein digest is separated and removed by filtration. Next, an atomic weight of copper mineral ions was calculated and added to 1,000 parts of the oligopeptide, a proteolytic product, and reacted to prepare a copper-oligopeptide. This was concentrated to about 35% solids and then powdered with a drum dryer or a spray dryer to obtain a powdered copper-oligopeptide.

＜실시예 2＞<Example 2>

실시예 1에서 얻은 분말 구리-올리고펩티드를 28%의 용액으로 조정하여 표 1과 같은 배합비로 구리강화음료를 만들었다. 구리의 권장량(RDA; Recommended Dietary Allowance)은 0.5∼3.0mg/day이므로 이를 기준으로 음료를 제조하였다.Powdered copper-oligopeptide obtained in Example 1 was adjusted to a solution of 28% to make a copper-reinforced beverage at the compounding ratio shown in Table 1. Since the recommended dietary allowance (RDA) of copper is 0.5-3.0 mg / day, a beverage was prepared based on this.

표 1 : 음료수의 조성물Table 1: Compositions of Drinking Water

성 분ingredient 첨가량Amount 28% 구리-올리고펩티드 용액28% copper-oligopeptide solution 1.0%1.0% 비타민-B1 Vitamin-B 1 0.01%0.01% 과 당Fructose 12%12% 비타민-CVitamin-C 0.03%0.03% 비타민-B2 Vitamin-B 2 0.01%0.01% 정제수Purified water 86.95%86.95% 합 계Sum 100%100%

＜실시예 3＞ <Example 3>

실시예 1에서 얻은 분말 구리-올리고펩티드를 표 2와 같은 배합으로 하고, 이를 캡슐에 0.5g 충진하여 구리 강화 건강식품을 제조하였다.Powdered copper-oligopeptide obtained in Example 1 was prepared as shown in Table 2, and 0.5g was filled in the capsule to prepare a copper-reinforced health food.

표 2. 구리 강화 건강식품 캡슐의 조성분Table 2. Composition of Copper Reinforced Health Food Capsule

성 분ingredient 첨가량Amount 분말 구리-올리고펩티드Powdered Copper-Oligopeptides 50.0%50.0% 비타민-B1 Vitamin-B 1 0.01%0.01% 콜 라 겐Collagen 49.95%49.95% 비타민-CVitamin-C 0.03%0.03% 비타민-B2 Vitamin-B 2 0.01%0.01% 합 계Sum 100%100%

흡수성 구리-올리고펩티드는 분자량이 800∼1,000으로 소장막의 흡수처인 융합단백질(Integral protein) 분자량인 24,000∼28,000 보다 작아서 흡수가 용이하고 수용성 단백질인 올리고펩티드가 구리 미네랄을 킬레이트(Chelate)하고 있어 소화관 내에 존재하는 식품성분들에 의해 장해를 받지 않아 흡수가 용이하다.Absorbent copper-oligopeptides have a molecular weight of 800-1,000 and are less than the molecular weight of 24,000-28,000, an integral protein absorber for small intestinal membranes. It is easy to absorb because it is not disturbed by the food ingredients present.

Claims (6)

단백질을 정제수에 현탁시키고, 여기에 단백분해효소를 2∼4중량％ 첨가하여 pH 3.5∼6.0에서 11시간∼12시간 반응시켜 올리고 펩티드를 얻는 단계와, 단백분해물인 올리고펩티드 1,000부에 대하여 구리미네랄 이온 1원자를 첨가하여 킬레이트반응시켜 구리-올리고펩티드를 얻는 단계를 포함함을 특징으로 하는 구리-올리고펩티드의 제조방법The protein was suspended in purified water, and 2-4% by weight of protease was added thereto to react for 11 to 12 hours at pH 3.5-6.0 to obtain an oligopeptide, and copper mineral was added to 1,000 parts of oligopeptide as a protein. Method for producing a copper- oligopeptide, comprising the step of obtaining a copper-oligopeptide by chelate reaction by adding one atom 삭제delete 제 1항에 있어서, 단백질은 식물성 또는 동물성 중에서 선택된 어느 하나이고, 단백분해효소는 펩신 또는 트립신 중에서 선택된 어느 하나이거나 둘 이상을 함유하는 것을 특징으로 하는 구리-올리고펩티드의 제조방법The method of claim 1, wherein the protein is any one selected from vegetable or animal, and the protease is any one selected from pepsin or trypsin, or a method for producing copper-oligopeptide, characterized in that it contains two or more. 삭제delete 삭제delete 삭제delete
KR10-2000-0039593A 2000-07-11 2000-07-11 Manufacturing Method of Absortive Copper-Oligopeptide in The Human Body KR100421464B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR10-2000-0039593A KR100421464B1 (en) 2000-07-11 2000-07-11 Manufacturing Method of Absortive Copper-Oligopeptide in The Human Body

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR10-2000-0039593A KR100421464B1 (en) 2000-07-11 2000-07-11 Manufacturing Method of Absortive Copper-Oligopeptide in The Human Body

Publications (2)

Publication Number Publication Date
KR20020006112A KR20020006112A (en) 2002-01-19
KR100421464B1 true KR100421464B1 (en) 2004-03-10

Family

ID=19677372

Family Applications (1)

Application Number Title Priority Date Filing Date
KR10-2000-0039593A KR100421464B1 (en) 2000-07-11 2000-07-11 Manufacturing Method of Absortive Copper-Oligopeptide in The Human Body

Country Status (1)

Country Link
KR (1) KR100421464B1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020084781A (en) * 2001-05-03 2002-11-11 지성규 Manufacturing Method of Absorptive Mineral-Oligopeptide by Enzyme in The Human Body and Health Food Using the Same
CN101500430B (en) 2006-08-07 2014-02-19 诺维信公司 Enzyme granules for animal feed
EP2051591B1 (en) 2006-08-07 2016-04-20 Novozymes A/S Enzyme granules for animal feed
KR102240111B1 (en) * 2019-06-26 2021-04-14 주식회사 팜스메틱 Cosmetic composition for moisturizing skin and improving skin wrinkles containing nano collagen peptide chelate mineral
KR102160726B1 (en) * 2019-06-26 2020-09-28 주식회사 팜스메틱 Uv-blocking and skin-moisturizing cosmetic composition containing nano collagen peptide chelate zinc

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5863898A (en) * 1996-10-29 1999-01-26 Incyte Pharmaceuticals, Inc. Human lim proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5863898A (en) * 1996-10-29 1999-01-26 Incyte Pharmaceuticals, Inc. Human lim proteins

Also Published As

Publication number Publication date
KR20020006112A (en) 2002-01-19

Similar Documents

Publication Publication Date Title
KR20080106906A (en) Mixed amino acid/mineral compounds having improved solubility
JP2007246413A (en) Composition containing milk-originated basic protein
KR20020006114A (en) Manufacturing Method of Absorptive Zinc-Oligopeptide in The Human Body
KR100729478B1 (en) Compositions containing peptide and electrolyte excretion promoter and foods containing the same
KR100421464B1 (en) Manufacturing Method of Absortive Copper-Oligopeptide in The Human Body
CN103535721A (en) Composition for improving glutathione concentration in human body and preparation method and application thereof
TWI377914B (en)
KR101229783B1 (en) method for extracting collagen composition from squid&#39;s endothelium for improving calcium absorptance, and whole soybean curd containing the collagen composition
JP4707401B2 (en) Antioxidant peptides from royal jelly
JP2011116761A (en) Antioxidative peptide derived from royal jelly
JP2945995B2 (en) Oligopeptide mixture, production thereof and nutrition feeding composition for patient of heptic disease
JPH03251162A (en) Peptide-containing drink, granule, powder, tablet and foaming agent
JPH06145061A (en) Anti-carcinoma cutaneum agent or food
JPH11193279A (en) Stabilizer for ascorbic acids, stabilization and preparation containing the same
EP0073173A1 (en) Products extracted from invertebrate sea-animals, their preparation and their uses in dietetics,pharmaceutics and cosmetology
JPH0898647A (en) Nourishing composition containing metal-binding casein blended therein
JPH05176712A (en) Food containing casein phosphopeptide
KR20100105561A (en) Food material for inhibiting bone resorption
JP2005097269A (en) Composition containing mung bean protein-decomposed material having angiotensin-converting enzyme inhibitory activity and active oxygen-removing activity
KIKUNAGA et al. The Bioavailabilit of Calcium in Spinach and Calcium-Oxalate to Calcium-Deficient Rats
RU2259147C2 (en) Dioactive additive
JP3022615B2 (en) Method for producing ε-polylysine
JPH104921A (en) Mineral solubilizing material
JPH03251161A (en) Peptide-containing drink, granule, powder, tablet and foaming agent
KR100421465B1 (en) Manufacturing Method of Absorptive Sodium-Oligopeptide Non-Electrolysis in Human Fluid

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
AMND Amendment
E902 Notification of reason for refusal
AMND Amendment
E601 Decision to refuse application
AMND Amendment
J201 Request for trial against refusal decision
B701 Decision to grant
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20070208

Year of fee payment: 4

LAPS Lapse due to unpaid annual fee