KR100411237B1 - Kit for Examining Water Quality - Google Patents
Kit for Examining Water Quality Download PDFInfo
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- KR100411237B1 KR100411237B1 KR10-2001-0029824A KR20010029824A KR100411237B1 KR 100411237 B1 KR100411237 B1 KR 100411237B1 KR 20010029824 A KR20010029824 A KR 20010029824A KR 100411237 B1 KR100411237 B1 KR 100411237B1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
- G01N31/227—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators for nitrates or nitrites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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Abstract
본 발명은 수성시료에 함유된 질소와 인의 농도를 측정하여, 시료의 오염여부 및 시료의 오염원인을 분석하는 수질조사 키트 및 전기 키트를 이용하여 수질오염의 여부 및 수질오염의 오염원인을 분석하는 방법에 관한 것이다. 본 발명의 수질조사 키트는 암모니아농도 측정시약, 아질산농도 측정시약, 질산농도 측정시약, 인산농도 측정시약, 측정항목별 표준비색띠 및 반응용기를 포함하고, 전기 수질조사 키트를 이용하여 수질오염의 여부 및 수질오염의 오염원인을 분석하는 방법은, 전기 키트에 포함된 암모니아농도 측정시약, 아질산농도 측정시약 및 질산농도 측정시약를 사용하여 시료에 함유된 암모니아, 아질산, 질산 및 인산의 농도를 측정하는 단계 및 시료에 함유된 암모니아, 아질산, 질산 및 인산의 농도를 비교하여, 시료의 오염여부와 정확한 오염원인을 판단하는 단계를 포함한다. 본 발명에 의하면, 수성시료에 함유된 질소를 형태별로 정확히 측정하여, 신속하고 정확하게 수질오염원인을 분석할 수 있으므로, 수질오염의 확산을 방지하는데 널리 활용될 수 있을 것이다.The present invention is to measure the concentration of nitrogen and phosphorus contained in the aqueous sample, using a water quality survey kit and electrical kit for analyzing whether the sample is contaminated and the cause of contamination of the sample to analyze the presence of water pollution and the cause of contamination of the water pollution It is about a method. The water quality survey kit of the present invention includes ammonia concentration measurement reagent, nitrite concentration measurement reagent, nitrate concentration measurement reagent, phosphoric acid concentration measurement reagent, standard colorimetric band and reaction vessel for each measurement item, and the use of electric water investigation kit The method of analyzing whether or not and the causes of water pollution are measured by measuring the concentration of ammonia, nitrous acid, nitric acid and phosphoric acid in the sample using the ammonia concentration reagent, the nitrite concentration reagent and the nitric acid concentration reagent included in the electric kit. And comparing the concentrations of ammonia, nitrous acid, nitric acid and phosphoric acid in the sample to determine whether the sample is contaminated and the exact cause of contamination. According to the present invention, since the nitrogen contained in the aqueous sample can be accurately measured for each form, the cause of water pollution can be analyzed quickly and accurately, and thus it can be widely used to prevent the spread of water pollution.
Description
본 발명은 수질조사 키트에 관한 것이다. 좀 더 구체적으로, 본 발명은 수성시료에 함유된 질소와 인의 농도를 측정하여, 시료의 오염여부 및 시료의 오염원인을 분석하는 수질조사 키트 및 전기 키트를 이용하여 수질오염의 여부 및 수질오염의 오염원인을 분석하는 방법에 관한 것이다.The present invention relates to a water survey kit. More specifically, the present invention measures the concentration of nitrogen and phosphorus contained in the aqueous sample, using the water quality survey kit and the electric kit to analyze whether the contamination of the sample and the cause of contamination of the sample and whether or not of water pollution It relates to a method of analyzing the cause of contamination.
근래에 와서, 환경에 대한 관심이 증대됨에 따라, 환경오염을 줄이려는 노력이 범 세계적으로 진행되고 있으며, 이러한 노력의 결과, 환경오염에 대한 연구결과가 지속적으로 발표되고 있다. 일반적으로, 환경오염은 크게 대기오염, 수질오염 및 토양오염으로 분류되는데, 이 중, 가장 쉽게 오염될 수 있는 것은 수질오염이라고 알려져 있다. 주로 호흡환경에 영향을 미치는 대기오염은 오염원이 공장, 자동차 등으로 한정되어 있어, 원천적인 봉쇄가 어느정도 가능하고, 토양오염의 경우, 오염의 확산속도가 빠르지 않기 때문에, 오염의 제거가 가능하나, 수질오염은 오염원이 한정되어 있지 않고, 오염의 확산속도가 빠르며, 토양오염을 유발시킬 수도 있기 때문에, 수질오염의 방지와 오염된 수질의 정화방법에 대하여 많은 연구가진행되고 있다.In recent years, as interest in the environment has increased, efforts to reduce environmental pollution have been made globally. As a result, research on environmental pollution has been continuously published. In general, environmental pollution is classified into air pollution, water pollution, and soil pollution. Among them, the most easily polluted is known as water pollution. Air pollution that mainly affects the respiratory environment is limited to the source of pollution, such as factories, automobiles, etc., the original blockade is possible to some extent, and in the case of soil pollution, it is possible to remove the pollution, because the spread of the pollution is not fast, Since water pollution is not limited to a source of pollution, the rate of spreading of the pollution is high, and soil pollution may be caused. Therefore, many studies have been conducted to prevent water pollution and to clean up polluted water.
한편, 공기, 수질 또는 토양 등의 어떠한 대상물이 오염되었는지 검사하기 위하여는, 시료를 채취하여 대상물의 오염도를 측정하고 있는데, 오염의 판정에 많은 시간이 소요되므로, 이를 개선하려는 노력이 계속되고 있다. 특히, 오염확산의 속도가 빠른 대기오염이나 수질오염의 경우, 검사시간을 단축하여야 할 필요성이 더욱 절실하게 요구되고 있다. 오염의 확산속도가 가장 빠른 대기오염의 경우, 대기 중에 함유된 오염물질이 복잡한 화합물을 형성하고 있지 않으므로, 상대적으로 쉽게 오염물질을 검출할 수 있으나, 수질오염의 경우, 오염물질의 확산속도가 빠르면서도, 오염물질이 복잡한 화합물을 형성할 수 있어, 정밀한 검사방법이 요구되므로, 오염현장에서는 단순히 수질에 함유된 질소와 인의 농도정도만을 측정할 수 있을 뿐, 더욱 정밀한 검사에는 많은 시간이 소요되는 실정이다. 더욱이, 수질오염의 주 오염원으로 알려진 질소는 수질환경에서 암모니아, 아질산 또는 질산의 형태로 존재하는데, 질소의 형태에 따라 수질오염의 정화방법이 달라지므로, 이들을 구별하여 검출하여야 하나, 지금까지 개발된 수성시료에 함유된 질소와 인의 농도를 측정하는 키트는 단순히 시료에 함유된 질소와 인의 양만을 측정할 수 있고, 하나의 정제형 고체시약 또는 한 종류의 액체시약의 형태를 취하고 있어, 검사된 질소 및 인의 농도의 오차가 커서, 단순히 오염의 여부판단 정도에만 사용되고 있는 실정이다.On the other hand, in order to check whether any object such as air, water quality or soil is contaminated, a sample is measured and the degree of contamination of the object is measured. Since determination of contamination takes a lot of time, efforts are being made to improve this. In particular, in the case of air pollution or water pollution, which has a rapid spread of pollution, the need to shorten the inspection time is urgently required. In the case of air pollution, which has the fastest spreading rate of pollution, pollutants contained in the air do not form complex compounds, and thus, pollutants can be detected relatively easily. However, in case of water pollution, the rate of diffusion of pollutants is high. In addition, since pollutants can form complex compounds, and precise inspection methods are required, only the concentration of nitrogen and phosphorus contained in the water can be measured at the polluting site. to be. Moreover, nitrogen, which is known as a major pollutant of water pollution, exists in the form of ammonia, nitrous acid, or nitric acid in the water environment. Since the method of purifying water pollution varies according to the form of nitrogen, these should be distinguished and detected. The kit for measuring the concentration of nitrogen and phosphorus in the aqueous sample can simply measure the amount of nitrogen and phosphorus in the sample and takes the form of one tablet solid reagent or one liquid reagent. And the error of the concentration of phosphorus is large, it is used only for the degree of contamination determination.
따라서, 수성시료에 함유된 여러 형태의 질소 및 인의 농도를 각각 정확하고 신속하게 검출할 수 있는 방법을 개발하여야 할 필요성이 끊임없이 대두되었다.Therefore, there is a constant need to develop a method capable of accurately and quickly detecting the concentrations of various types of nitrogen and phosphorus in aqueous samples, respectively.
이에, 본 발명자들은 수성시료에 함유된 여러 형태의 질소 및 인의 농도를 각각 정확하고 신속하게 검출할 수 있는 방법을 개발하고자 예의 연구노력한 결과, 암모니아, 아질산, 질산 및 인산의 농도를 신속하고 정확하게 측정할 수 있는 시약을 포함하는 수질조사 키트를 이용할 경우, 수질오염이 오염원인을 신속하고 정확하게 판단할 수 있음을 확인하고, 본 발명을 완성하게 되었다.Accordingly, the present inventors have made intensive studies to develop a method capable of accurately and rapidly detecting the concentrations of various types of nitrogen and phosphorus contained in an aqueous sample, and as a result, the concentration of ammonia, nitrous acid, nitric acid and phosphoric acid is measured quickly and accurately. When using a water investigation kit containing a reagent that can be confirmed that the water pollution can determine the cause of contamination quickly and accurately, the present invention was completed.
결국, 본 발명의 주된 목적은 암모니아, 아질산, 질산 및 인산의 농도를 측정할 수 있는 시약을 포함하는 수질조사 키트를 제공하는 것이다.After all, the main object of the present invention is to provide a water investigation kit comprising a reagent capable of measuring the concentration of ammonia, nitrous acid, nitric acid and phosphoric acid.
본 발명의 다른 목적은 전기 키트를 이용하여 수질오염의 오염원인을 분석하는 방법을 제공하는 것이다.Another object of the present invention is to provide a method for analyzing the cause of contamination of water pollution using an electric kit.
본 발명의 수질조사 키트는 2 내지 10%(w/v)의 하이포아염소산나트륨(sodium hypochlorite) 수용액인 A1시약, 니트로 프루시드 나트륨과 충진제가 1:9(w/w)로 혼합된 A2시약 및 살리실산을 1 내지 5%(v/v) 포함하고 유기용매를 5 내지 20%(v/v) 포함하는 수용액인 A3시약으로 구성된 암모니아농도 측정시약; 술퍼제(sulfanilamide)를 3 내지 7%(v/v) 포함하고 유기용매를 5 내지 15%(v/v) 포함하는 수용액인 B1시약 및 5 내지 15g/L의 농도를 가지는 N-(1-naphthyl)ethylenediamine dihydrochloride(NED) 수용액인 B2시약으로 구성된 아질산농도 측정시약; 아연과 충진제가 1:1(w/w)로 혼합된 C1시약, 2 내지 3g/L의 술퍼닐산(sulfanilic acid) 수용액인 C2시약, 0.5 내지 1.5g/L의 나프틸디아민(1,4-Naphthyldiamine) 수용액인 C3시약으로 구성된 질산농도 측정시약; 5 내지 15g/L의 암모늄 몰리브덴 산염(ammonium molybdate) 수용액인 D1시약 및 40 내지 60g/L의 아스코르빈산과 1 내지 4%(v/v)의 염산을 함유하고 유기용매를 2 내지 8%(v/v) 포함하는 수용액인 D2시약으로 구성된 인산농도 측정시약; 측정항목별 표준비색띠; 및, 반응용기를 포함한다: 이때, 사용되는 충진제로는 차콜(charcoal), 실리카겔(silica gel) 또는 벤토나이트(bentonite)를 사용함이 바람직하고, 유기용매로는 메탄올, 에탄올, 디클로로메탄, 클로로포름, 아세톤 또는 디옥산을 사용함이 바람직하다. 또한, 표준비색띠는 암모니아, 아질산, 질산 또는 인산의 농도변화에 따른 색변화양상을 도시한 기준표이고, 반응용기는 색변화를 쉽게 알아볼 수 있도록, 투명한 재질로 제조됨이 바람직하고, 별도의 측정장치가 필요하지 않도록, 투명한 재질의 용기의 외부에 시료의 첨가량을 나타내는 눈금과 각 시약 중 앵상 시약이 첨가되는 눈금이 표시됨이 바람직하다. 아울러, 휴대가 간편하도록 전기 시약들은 빛과 습기를 차단할 수 있는 재질의 포장재로 포장하고 밀봉하는 것이 바람직하다.The water investigation kit of the present invention is an A1 reagent of 2 to 10% (w / v) sodium hypochlorite aqueous solution, an A2 reagent mixed with 1: 9 (w / w) of nitroproxide sodium and a filler. And an ammonia concentration measuring reagent consisting of A3 reagent which is an aqueous solution containing 1 to 5% (v / v) of salicylic acid and 5 to 20% (v / v) of an organic solvent. B-reagent which is an aqueous solution containing 3 to 7% (v / v) of sulffanilamide and 5 to 15% (v / v) of organic solvent and N- (1- having a concentration of 5 to 15 g / L nitrite concentration measurement reagent consisting of B2 reagent which is an aqueous solution of naphthyl) ethylenediamine dihydrochloride (NED); C1 reagent mixed with zinc and filler in 1: 1 (w / w), C2 reagent in aqueous solution of 2 to 3 g / L sulfanilic acid, 0.5 to 1.5 g / L naphthyldiamine (1,4- Naphthyldiamine) Nitric acid concentration reagent consisting of C3 reagent solution; 5 to 15 g / L ammonium molybdate aqueous solution of D1, 40 to 60 g / L ascorbic acid and 1 to 4% (v / v) hydrochloric acid, and 2 to 8% organic solvent v / v) a phosphoric acid concentration measuring reagent consisting of D2 reagent which is an aqueous solution containing; Standard colorimetric band by measurement item; And a reaction vessel. Charcoal, silica gel or bentonite is preferably used as the filler, and organic solvents include methanol, ethanol, dichloromethane, chloroform and acetone. Or dioxane is preferred. In addition, the standard color band is a reference table showing the color change pattern according to the change in the concentration of ammonia, nitrous acid, nitric acid or phosphoric acid, the reaction vessel is preferably made of a transparent material, so that the color change can be easily recognized, the separate measurement In order to avoid the need for an apparatus, it is preferable that a scale indicating the amount of the sample added to the outside of the container made of a transparent material, and a scale on which the reagents of each reagent are added. In addition, the electrical reagents are preferably packaged and sealed in a packaging material that can block light and moisture for easy portability.
전기, 본 발명의 수질조사 키트를 이용하여, 수질오염의 여부 및 수질오염의오염원인을 분석하는 방법은, 수성시료에 전기 키트에 포함된 암모니아농도 측정시약의 A1 시약, A2 시약 및 A3 시약을 순차적으로 혼합하여 반응시키고, 표준비색띠를 이용하여 수성시료에 함유된 암모니아의 농도를 측정하는 단계; 수성시료에 전기 키트에 포함된 아질산농도 측정시약의 B1 시약 및 B2 시약을 순차적으로 혼합하여 반응시키고, 표준비색띠를 이용하여 수성시료에 함유된 아질산의 농도를 측정하는 단계; 수성시료에 전기 키트에 포함된 질산농도 측정시약의 C1 시약, C2 시약 및 C3 시약을 순차적으로 혼합하여 반응시키고, 표준비색띠를 이용하여 수성시료에 함유된 질산의 농도를 측정하는 단계; 수성시료에 전기 키트에 포함된 인산농도 측정시약의 D1 시약 및 D2 시약을 순차적으로 혼합하여 반응시키고, 표준비색띠를 이용하여 수성시료에 함유된 인산의 농도를 측정하는 단계; 및, 전기 측정된 암모니아, 아질산, 질산 및 인산의 농도를, 오염되지 않은 수성시료의 암모니아, 아질산, 질산 및 인산의 농도와 비교하여 수질오염원을 판별하는 단계를 포함한다.The method of analyzing the presence of water pollution and the cause of water pollution using the water quality survey kit of the present invention includes: A1 reagent, A2 reagent, and A3 reagent of the ammonia concentration measurement reagent included in the electric kit in an aqueous sample. Reacting by sequentially mixing and measuring the concentration of ammonia contained in the aqueous sample using a standard colorimetric band; Reacting the aqueous sample by sequentially mixing the B1 reagent and the B2 reagent of the nitrite concentration measurement reagent included in the electric kit, and measuring the concentration of the nitrous acid contained in the aqueous sample using a standard colorimetric band; Reacting the aqueous sample by sequentially mixing the C1 reagent, the C2 reagent, and the C3 reagent of the nitrate concentration measurement reagent included in the kit, and measuring the concentration of nitric acid contained in the aqueous sample using a standard colorimetric band; Reacting the aqueous sample by sequentially mixing the D1 and D2 reagents of the phosphate concentration measurement reagent included in the electric kit, and measuring the concentration of phosphoric acid in the aqueous sample using a standard colorimetric band; And determining the source of water pollution by comparing the concentrations of ammonia, nitrous acid, nitric acid and phosphoric acid measured previously with the concentrations of ammonia, nitrous acid, nitric acid and phosphoric acid in the uncontaminated aqueous sample.
이하, 실시예를 통하여 본 발명을 보다 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .
실시예 1: 수질조사 키트의 제작 Example 1 Preparation of Water Quality Survey Kit
암모니아농도 측정시약, 아질산농도 측정시약, 질산농도 측정시약 및 인산농도 측정시약를 제조하고, 표준비색띠 및 반응용기를 갖추어 수질조사 키트를 제작하였다.Ammonia concentration reagent, nitrite concentration reagent, nitrate concentration reagent and phosphoric acid concentration reagent were prepared, and standard colorimetric band and reaction vessel were prepared to prepare a water quality survey kit.
실시예 1-1: 암모니아농도 측정시약의 작제 Example 1-1 Preparation of Ammonia Concentration Reagent
5%(w/v)의 하이포아염소산나트륨 수용액 100㎖를 A1시약으로, 니트로 프루시드 나트륨 10g과 차콜 90g을 혼합한 혼합물을 A2시약으로, 살리실산 3㎖과 메탄올 17㎖이 혼합된 수용액 100㎖을 A3시약으로 제조하고, 각 시약을 폴리프로필렌 재질의 불투명 용기에 포장하고 밀봉하여 암모니아농도 측정시약을 작제하였다.100 ml of a 5% (w / v) sodium hypochlorite aqueous solution as an A1 reagent, a mixture of 10 g of nitroproxide sodium and 90 g of charcoal as an A2 reagent, 100 ml of an aqueous solution of 3 ml of salicylic acid and 17 ml of methanol Was prepared with an A3 reagent, and each reagent was packed in an opaque container made of polypropylene and sealed to prepare an ammonia concentration measuring reagent.
실시예 1-2: 아질산농도 측정시약의 작제 Example 1-2 : Preparation of nitrite concentration measurement reagent
5mg 술퍼제(sulfanilamide)와 10㎖ 에탄올을 포함하는 수용액 100㎖을 B1시약으로, 10g/L의 NED수용액 100㎖를 B2시약으로 제조하고, 각 시약을 폴리프로필렌 재질의 불투명 용기에 포장하고 밀봉하여 아질산농도 측정시약을 작제하였다.100 ml of an aqueous solution containing 5 mg sulfanamide and 10 ml ethanol were prepared with B1 reagent, 100 ml of 10 g / L NED aqueous solution was prepared with B2 reagent, and each reagent was packaged and sealed in a polypropylene opaque container. A nitrite concentration measuring reagent was prepared.
실시예 1-3: 질산농도 측정시약의 작제 Example 1-3 Preparation of Nitric Acid Concentration Reagent
미세아연분말 10g과 차콜 10이 혼합된 것을 C1시약으로, 2.5g/L의술퍼닐산(sulfanilic acid)의 수용액 100㎖를 C2시약으로, 1g/L의 나프틸디아민(1,4-naphthyldiamine)의 수용액 100㎖를 C3시약으로 제조하고, 각 시약을 폴리프로필렌 재질의 불투명 용기에 포장하고 밀봉하여 질산농도 측정시약을 작제하였다.10 g of fine zinc powder and 10 charcoal are mixed with C1 reagent, 100 ml of 2.5 g / L aqueous solution of sulfanilic acid is converted into C2 reagent, and 1 g / L of naphthyldiamine (1,4-naphthyldiamine) is used. 100 ml of an aqueous solution was prepared with C3 reagent, and each reagent was packed in an opaque container made of polypropylene and sealed to prepare a nitric acid concentration measuring reagent.
실시예 1-4: 인산농도 측정시약의 작제 Example 1-4 Preparation of Phosphoric Acid Concentration Reagent
10g/L의 암모늄 몰리브덴 산염(ammonium molybdate)인 D1시약과, 50g/L의 아스코르빈산과 2.5%(v/v)의 염산을 함유하고 에탄올을 5%(v/v) 포함하는 D2시약을 제조하고, 각 시약을 폴리프로필렌 재질의 불투명 용기에 포장하고 밀봉하여 인산농도 측정시약을 작제하였다.10 g / L ammonium molybdate D1 reagent, 50 g / L ascorbic acid and 2.5% (v / v) hydrochloric acid and 5% (v / v) ethanol. Each reagent was packaged in an opaque container made of polypropylene and sealed to prepare a phosphoric acid concentration measuring reagent.
실시예 2: 수질조사 키트의 정확성 검증 Example 2 Verification of Accuracy of Water Quality Survey Kit
실시예 1에서 제작한 수질조사 키트의 정확도를 검증하기 위하여, 동일시료를 본 발명의 수질조사 키트로 분석하고, 이온크로마토그래피로 분석하여, 두 가지 분석결과를 비교하여, 본 발명의 수질조사 키트의 정확성을 검증하였다.In order to verify the accuracy of the water investigation kit prepared in Example 1, the same sample was analyzed by the water irradiation kit of the present invention, and analyzed by ion chromatography, and the two analysis results were compared, and the water investigation kit of the present invention. The accuracy of the was verified.
한강 하류에서 3 지점에서 수집한 검사시료 A, B, C를 각각 10㎖씩 반응용기에 넣고, 암모니아농도 측정시약의 A1 시약 10㎖, A2 시약 10g, A3 시약 10㎖를 순차적으로 첨가한 후, 반응용기내에서 10분간 혼합한 후, 반응액의 색을 표준비색띠에 비교하여 암모니아의 농도를 측정하였다. 또한, 전기 검사시료 A, B, C를 각각 10㎖씩 반응용기에 넣고, 아질산농도 측정시약의 B1 시약 10㎖과 B2 시약 10㎖을 순차적으로 첨가한 다음, 반응용기내에서 10분간 혼합한 후, 반응액의 색을 표준비색띠에 비교하여 아질산의 농도를 측정하였다. 다음으로, 전기 검사시료 A, B, C를 각각 10㎖씩 반응용기에 넣고, 질산농도 측정시약의 C1 시약 10g, C2 시약 10㎖ 및 C3시약 10㎖를 순차적으로 첨가한 다음, 반응용기내에서 10분간 혼합한 후, 반응액의 색을 표준비색띠에 비교하여 질산의 농도를 측정하였다. 아울러, 전기 검사시료 A, B, C를 각각 10㎖씩 반응용기에 넣고, 인산농도 측정시약의 D1시약 10㎖ 및 D2시약 10㎖를 순차적으로 첨가한 다음, 반응용기내에서 10분간 혼합한 후, 반응액의 색을 표준비색띠와 비교하여 인산의 농도를 측정하였다.10 ml of test samples A, B, and C collected at three points downstream of the Han River were placed in a reaction container, and 10 ml of A1 reagent, 10 g of A2 reagent, and 10 ml of A3 reagent of the ammonia concentration reagent were sequentially added. After mixing for 10 minutes in the reaction vessel, the concentration of the ammonia was measured by comparing the color of the reaction solution with the standard colorimetric band. In addition, 10 ml of each of the test samples A, B, and C were placed in the reaction vessel, 10 ml of the B1 reagent and 10 ml of the B2 reagent of the nitrite concentration reagent were added sequentially, and then mixed in the reaction vessel for 10 minutes. The concentration of nitrous acid was measured by comparing the color of the reaction solution with a standard colorimetric band. Next, 10 ml of each test sample A, B, and C were placed in a reaction vessel, and 10 g of C1 reagent, 10 ml of C2 reagent, and 10 ml of C3 reagent of nitrate concentration measurement reagent were added sequentially, and then in the reaction vessel. After mixing for 10 minutes, the concentration of nitric acid was measured by comparing the color of the reaction solution with a standard colorimetric band. In addition, 10 ml of each test sample A, B, and C were put into the reaction vessel, 10 ml of D1 reagent and 10 ml of D2 reagent of the phosphate concentration measurement reagent were added sequentially, and then mixed in the reaction vessel for 10 minutes. The concentration of phosphoric acid was measured by comparing the color of the reaction solution with the standard colorimetric band.
비교실시예 1: 이온크로마토그래피를 이용한 암모니아, 아질산, 질산 및 인산농도의 측정 Comparative Example 1 Measurement of Ammonia, Nitrous Acid, Nitric Acid and Phosphoric Acid Concentration by Ion Chromatography
실시예 2에서 사용한 동일한 시료를 이온크로마토그래피(HIC 6A, Shimadzu, Japan)로 분석(시료전처리: Acrodisc LC13PVDF filter(0.45㎛), 이동상:0.85mM NaHCO3/0.9mM Na2CO3, 유속:1.0mL/min)하여 암모니아, 아질산, 질산 및 인산의 농도를 측정한 다음, 실시예 2의 결과와 측정치를 비교하였다(참조: 표 1).The same sample used in Example 2 was analyzed by ion chromatography (HIC 6A, Shimadzu, Japan) (sample pretreatment: Acrodisc LC13 PVDF filter (0.45 μm), mobile phase: 0.85 mM NaHCO 3 /0.9 mM Na 2 CO 3 , flow rate: 1.0 mL / min) to measure the concentrations of ammonia, nitrous acid, nitric acid and phosphoric acid, and then compare the results with those of Example 2 (see Table 1).
* Kt: 본 발명의 수질조사 키트로 검사한 농도* Kt: concentration tested by the water quality kit of the present invention
Ic: 이온크로마토그래피로 검사한 농도Ic: concentration determined by ion chromatography
본 발명의 수질조사 키트로 측정된 값과 이온크로마토그래피로 측정된 값은 20%의 오차를 나타내었다. 일반적으로, ppm값을 측정할 경우, 30%의 오차범위는 허용가능한 표준편차범위로 간주되므로, 본 발명의 수질조사 키트로 측정된 값은 허용가능한 측정값으로 간주될 수 있었다.The value measured by the water quality irradiation kit of the present invention and the value measured by ion chromatography showed an error of 20%. In general, when the ppm value is measured, an error range of 30% is regarded as an acceptable standard deviation range, so that the value measured with the water quality kit of the present invention could be regarded as an acceptable measurement value.
이상에서 상세히 설명하고 입증하였듯이, 본 발명은 수성시료에 함유된 질소와 인의 농도를 측정하여, 시료의 오염여부 및 시료의 오염원인을 분석하는 수질조사 키트 및 전기 키트를 이용하여 수질오염의 여부 및 수질오염의 오염원인을 분석하는 방법을 제공한다. 본 발명의 수질조사 키트는 암모니아농도 측정시약, 아질산농도 측정시약, 질산농도 측정시약, 인산농도 측정시약, 측정항목별 표준비색띠 및 반응용기를 포함하고, 전기 키트를 이용하여 측정된 수성시료의 암모니아 농도, 아질산 농도, 질산 농도 및 인산농도를 오염되지 않은 수성시료의 암모니아 농도,아질산 농도, 질산 농도 및 인산농도와 비교하여 오염원인을 분석한다. 본 발명에 의하면, 수성시료에 함유된 질소를 형태별로 측정하여, 신속하게 수질오염원인을 분석할 수 있으므로, 수질오염의 확산을 방지하는데 널리 활용될 수 있을 것이다.As described and demonstrated in detail above, the present invention measures the concentration of nitrogen and phosphorus contained in the aqueous sample, using the water quality survey kit and electric kit to analyze whether the sample is contaminated and the cause of contamination of the sample, Provides a method for analyzing the causes of water pollution. The water quality investigation kit of the present invention includes ammonia concentration measurement reagent, nitrite concentration measurement reagent, nitrate concentration measurement reagent, phosphoric acid concentration measurement reagent, standard colorimetric band for each measurement item, and reaction vessel, and measured of the aqueous sample measured using the electric kit. The cause of contamination is analyzed by comparing the ammonia concentration, nitrite concentration, nitric acid concentration and phosphate concentration with the ammonia concentration, nitrite concentration, nitric acid concentration and phosphate concentration of the uncontaminated aqueous sample. According to the present invention, by measuring the nitrogen contained in the aqueous sample by form, it is possible to quickly analyze the cause of water pollution, it will be widely used to prevent the spread of water pollution.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101925544B1 (en) | 2018-07-02 | 2018-12-05 | 주식회사 코비 | Ammonia measuring system having auto-backwashing pretreatment apparatus and constant-temperature detector |
| KR101984812B1 (en) | 2018-11-16 | 2019-06-03 | 박종택 | Apparatus for injecting reagent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108152286A (en) * | 2018-02-11 | 2018-06-12 | 广东出入境检验检疫局检验检疫技术中心 | A kind of kit and its detection method of quick qualification determination nitrite in cubilose content |
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|---|---|---|---|---|
| JPS58137754A (en) * | 1982-02-09 | 1983-08-16 | Suzuken Kk | Reagent for inspection of water quality |
| US4812413A (en) * | 1987-03-17 | 1989-03-14 | Erez Forensic Technology, Ltd. | Reagent, process and kit for drug detection |
| KR940020118A (en) * | 1993-02-06 | 1994-09-15 | 박형인 | Method of Determination of Purity in Aerobic Treatment of Organic Sewage |
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2001
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58137754A (en) * | 1982-02-09 | 1983-08-16 | Suzuken Kk | Reagent for inspection of water quality |
| US4812413A (en) * | 1987-03-17 | 1989-03-14 | Erez Forensic Technology, Ltd. | Reagent, process and kit for drug detection |
| KR940020118A (en) * | 1993-02-06 | 1994-09-15 | 박형인 | Method of Determination of Purity in Aerobic Treatment of Organic Sewage |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101925544B1 (en) | 2018-07-02 | 2018-12-05 | 주식회사 코비 | Ammonia measuring system having auto-backwashing pretreatment apparatus and constant-temperature detector |
| KR101984812B1 (en) | 2018-11-16 | 2019-06-03 | 박종택 | Apparatus for injecting reagent |
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