KR100394907B1 - Gastric Stimulating Betaine·Hydrochloric Acid and Protease Feed Additive - Google Patents

Abstract

The present invention is a feed additive for gastrointestinal function promoting gastrointestinal function promoting feed additive comprising 0.1 to 0.2% by weight hydrochloric acid and betaine and pepsin as a protease in the daily feed amount of the formula It is done.

Animals fed the gastrointestinal stimulation feed additive according to the present invention has the advantage that gastric acid secretion is enhanced, digestion rate is greatly improved by reducing the number of microorganisms, such as enteric coliforms.

Description

Gastric Stimulating BetaineHydrochloric Acid and Protease Feed Additive}

The present invention relates to a feed additive for promoting gastrointestinal function, and relates to a feed additive for promoting gastrointestinal function using 0.1 to 0.2% by weight of hydrochloric acid and betaine and pepsin as a protease as an active ingredient in a daily feed amount. will be.

In general, the acidity in the stomach of unit animals including humans is pH 2-4, the small intestine is pH 5-6, the large intestine is raised to pH 7. This high acidity in the stomach is maintained by hydrochloric acid (HCl) secreted in the stomach. Hydrochloric acid (HCl) is secreted from gastric mucosa (parietal cells), in humans secreted 2-3 (L) per day, pH is 0.87. Hydrochloric acid maintains high acidity in the stomach, denaturing ingested proteins, activating pepsinogen to pepsin, and also helping to absorb calcium and iron (Fe ²⁺ ), Hydrolyzes a small amount of disaccharides, promotes secretion of cyclin, a digestive hormone from the duodenum, gastric acid in the stomach, inhibits fermentation and decay by harmful microorganisms, promotes bicarbonate secretion in the pancreas, and goes down to the duodenum from the stomach Acid regulates the release of gastric contents.

However, if the pH of the stomach is above 6 due to lack of hydrochloric acid, the activity of protease may decrease, and the growth of pathogenic bacteria such as Helico bacteria increases. This can happen in young animals whose digestive organs are not yet fully developed. For example, in piglets, the pH in the stomach remains from 4.1 to 5.7, regardless of the type of feed fed after weaning, from birth to 1 to 2 weeks after weaning, as shown in Table 1.

Changes in pH of the stomach with increasing age of piglets Feed type fed Age after birth (days) 28 35 42 Dairy Solid Feed 5.7 4.1 5.2 Dairy Liquid Feed 4.1 4.6 4.6 Corn-Soybean Meal Solid Feed 4.3 4.8 4.4 Average 4.7 4.5 4.7 (Efird et al., 1982)

This level of pH is a so-called gastric acid deprivation state that is higher than 2.5-3.0, which is an appropriate pH for converting pepsinogen, a protease, into pepsin. This can lead to the following problems: ① insufficient digestion of vegetable protein and energy sources supplied after weaning, ② balance of the intestinal microflora, ③ digestion in the small intestine and vigorous in the large intestine Microbial fermentation occurs to cause diarrhea due to osmotic imbalance, ④ diarrhea due to the reproduction of E. coli can occur. In addition, various amino acids, which are end products decomposed by pepsin in the stomach, promote the secretion of proteolytic enzymes of the pancreas, and gastric acid itself acts as an accelerator to secrete bicarbonate of the pancreas. Since acid descending from the stomach to the duodenum regulates gastric emptying, it is very important to maintain acid secretion and therefore proper acidity (Ravindran and Kornegay, 1993).

In general, people grow older and have certain diseases, such as dementia, or if they are physically disabled, gastric acid secretion is reduced and acidity in the stomach decreases. In the United States, 30% of older people over 60 years of age have little or no gastric acid secretion due to atrophic gastritis. If the acidity in the stomach is lowered, the acidity in the stomach is lowered, resulting in poor nutritional and pathological results. That is, ① As the pH increases, the protein degradation ability decreases, so that the degradation of protein is reduced, and hypersensitivity reactions and allergy symptoms appear on the ingested protein. ② As soon as the food is ingested, bloating, belching, and burning. ) And flatulence, ③ dyspepsia, diarrhea and constipation, ④ feeling bloated for a long time after ingesting food, ⑤ nausea symptoms after taking medicines or supplements, ⑥ Rectal pruritus may occur, ⑦ physiological deficiency due to absorption of iron, calcium, zinc, vitamin B group and folic acid (folic acid) may appear, ⑧ parasites in the digestive tract and chronic candida may occur ( Silagle, 1998).

In the prior art, a single powder is mainly used as one of the solutions to the above problems. In other words, citric acid, fumaric acid, formic acid, and propionic acid are commonly used as animal powders. Other malic acid, sodium- n- Organic acids such as butyrate (sodium- n- butyrate) and lactic acid, and inorganic acids such as hydrochloric acid, sulfuric acid, and phosphoric acid.

The technical problem to be achieved by the present invention is to solve the problems of reduction of digestion rate, increase in intestinal harmful Escherichia coli, decrease in the growth rate of animals, etc. due to the decrease in the secretion function of conventional gastric acid, hydrochloric acid, betaine and pepsin which is a protease To provide a feed additive for the promotion of gastrointestinal function.

The present invention for achieving the above technical problem, characterized in that it comprises a hydrochloric acid and betaine, a protease and an excipient (carrier) composed of the following formula as a feed additive for promoting gastrointestinal function.

More specifically, the main component of the feed additive for gastrointestinal function is 520 mg / g of hydrochloric acid and betaine, 200 mg / g of pepsin as a protease and 280 mg / g of an excipient (carrier). It is done.

Hereinafter will be described in detail on the basis of a specific embodiment of the present invention and the experimental analysis table to prove its effect.

In order to investigate the feeding effect of the gastrointestinal function-promoting feed additive containing the hydrochloric acid, betaine and protease of the present invention, 28 rats having an average weight of 91 g were subjected to a breeding test by a full random batch method. The breeding period was 3 weeks. Test feeds were formulated using normal feed as shown in Table 2, and crude protein and metabolic energy contents were 19% and 3,174㎉ / kg during the first week (phase I) after the start of the test. During II), crude protein and metabolic energy contents were 16% and 3,172 kW / kg.

At the end of the breeding test, blood was collected from three rats per treatment group to examine blood characteristics. Three mice per treatment were sacrificed for stomach, liver, pancreas, small intestine and colon to examine intestinal digestive enzymes, intestinal morphology (villi height and fusion depth), organ weight and microbial count. In addition, anesthetized three rats per treatment group for gymnastic analysis, and then slaughtered and stored at -20 ℃.

The environmental temperature of the test animal breeding room shall be maintained at an average of 22 ± 1 ℃ and a relative humidity of 65 ± 5%, and the contrast shall be adjusted in a 12 hour period (08: 00∼20: 00). Water and feed were freed for the entire test period. Seven days before the end of the test, three repetitions of each treatment were placed in the metabolism cage, and then, after four days of preliminary period required for metabolic cage adaptation, three minutes were collected to remove foreign substances and the total weight was measured. Collected minutes were lyophilized and crushed and stored frozen until analysis, and the general components were analyzed according to the AOAC (1990) method.

The stomach, pancreas and small intestine were opened and removed, and the fat attached to organs was removed cleanly, washed with physiological saline (0.9% NaCl) to remove blood, and drained with filter paper, and then weighed and measured.

The activity of lactase, maltase, and sucrase was measured by Dahlqvist method, and disaccharide degrading enzyme activity was expressed as specific activity (unit of activity / protein g), and the protein content of enzyme solution showed bovine serum albumin. Using as a standard, it was quantified by the Lowry method.

The chorillary height and depth of the intestine of the small intestine were cut into three parts (the duodenum, jejunum, and ileum) by removing the contents using the Pluske method, followed by 10% phosphate-buffer formalin (pH). 7.4) was fixed for about 10 days and observed with an optical microscope.

Intestinal microbial analysis was performed by diluting the contents of the colon in 0.1% peptone water and diluting them at a constant rate, and then diluting the diluted solution with Entero Bacteriaceae Count Plate Petrifilm and E. Coli Count Plate Petrifilm (Microbiology). Products (3M Health Care, USA; AOAC, 1995) were inoculated with 1 ml of intestinal bacteria ( Entero Bacteriaceae ) at 35 ° C. for 24 hours and E. Coli at 48 ° C. for 48 hours to count colonies. .

Blood was collected from the heart by anesthesia with ethyl ether, placed in a tube treated with sodium heparin, and then tested for leukocytes and red blood cells with a blood analyzer without blood clotting. Analyzed.

Feed Formulation Table and Nutrient Content Raw feed or nutrient content Phase I (0-7 days) Phase II (8 ~ 21 days) Control Gastrointestinal Function Control Gastrointestinal Function Raw material feed (%): wheat 47.80 47.80 52.45 52.44 Soybean meal 24.70 24.70 13.73 13.91 Alfalfa 3.20 3.20 3.74 3.33 corn 17.03 16.93 22.17 22.13 Fishmeal 1.50 1.50 2.00 2.00 Soybean oil 1.50 1.50 1.28 1.35 Salt 0.60 0.60 0.60 0.60 Lysine - - 0.20 0.20 Choline chloride 0.30 0.30 0.30 0.30 Calcium phosphate 1.53 1.53 0.88 0.88 Limestone 1.34 1.34 2.15 2.16 Vitamin mineral 0.50 0.50 0.50 0.50 Gastrointestinal Promoting Feed Additives - 0.10 - 0.20 Nutrients Content: Metabolic energy (㎉ / ㎏) 3,176.05 3,172.63 3,170.39 3,173.54 Crude protein 19.25 19.24 16.01 16.02 Lysine 1.02 1.02 0.93 0.93 Methionine + Cystine 0.60 0.60 0.58 0.58 calcium 1.20 1.20 1.20 1.20 sign 0.62 0.62 0.62 0.62

As shown in Table 3, the feed additive for gastrointestinal function promotion used in the test of the present invention contains 520 mg of hydrochloric acid and betaine, 200 mg of pepsin, a protease, and 280 mg of an excipient (carrier). 0.6 g (0.1 to 0.2% level in the diet) was carried out in one tablet.

Feed additive composition table for gastrointestinal function promotion ingredient Content (mg / g) Hydrochloric acid 520 Pepsin 200 Excipient 280

1) Growth capacity improvement effect

In order to investigate the effect of gastrointestinal feed additives on the growth ability of rats, 28 rats with an average weight of 91 g were subjected to a breeding test. As shown in Table 4 below, the dietary supplements for gastrointestinal function were ingested. The growth rate of rats in the test group was improved by about 10% compared to the control (p <0.05), and feed intake and feed efficiency were also improved by about 5% compared to the control. These results are also observed in Table 5. Han et al. (2000b) showed that the growth capacity and feed efficiency of powder-fed test plots were examined in a piglet experiment to investigate the effect of feed additives for gastrointestinal function on growth ability. Significant improvement over the control. In addition, the improvement effect was more significant within 2 weeks after weaning due to the fact that gastric acid secretion ability was lower in piglets with low age compared to those with high age.

Effect of Gastrointestinal Feed Additives on Growth Ability of Rats Treatment Starting weight (g) End weight (g) Daily weight gain (g / day) Intake (g / day) Feed efficiency Control 90.9 250.8 ^b 7.62 ^b 22.63 2.98 Test 91.0 267.0 ^a 8.38 ^a 24.06 2.87 Standard error 2.51 5.62 0.20 0.48 0.03 ^{a, b} Different subscripts on the same line have significant differences at p <0.05. (Han et al. 2000a)

Effect of Gastrointestinal Feed Additives on Growth Capacity of Weaning Pigs 0-14 days after weaning 0-21 days after weaning Control Test Control Test Daily weight gain (g) 177 211 239 268 Feed efficiency 1.69 ^a 1.30 ^b 1.60 ^a 1.36 ^{b a, b} Different subscripts on the same line have significant differences at p <0.05. (Han et al. 2000b)

2) Improvement of digestibility of nutrients

Table 6 below shows the effect of feed additives for the promotion of gastrointestinal function based on hydrochloric acid, betaine and protease, on the digestibility of nutrients. As shown in Table 5, the digestibility of dry matter, crude protein and energy of the test group fed the dietary supplements containing hydrochloric acid, betaine and pepsin tended to be higher than that of the control. A similar result is shown in Table 7. Han et al. (2000b) fed a feed supplemented with a powder that promotes gastrointestinal function to weaned pigs with weak gastric acid secretion due to insufficient development of digestive organs. The improvement was 5.02 to 6.44% and 1.84 to 5.06%, respectively. These results suggest that feeding hydrochloric acid, betaine and pepsin to young rats promotes gastrointestinal function and consequently improves the digestibility of nutrients, especially proteins.

Effect of feed additives for gastrointestinal function on digestibility of dry matter, total energy and crude protein in rats (%) Treatment building(%) Total energy (㎉ / ㎏) Crude Protein (%) Control 81.84 81.48 78.88 Test 83.06 83.50 79.60 Standard error 0.30 1.02 0.29 (Han et al. 2000a)

Effect of Gastrointestinal Feed Additives on Dry matter and Crude Protein Digestibility of Weaning Pigs (%) 11-14 days after weaning 18 to 21 days after weaning Control Test Control Test building 79.94 ^a 83.96 ^a 77.94 ^b 82.96 ^a Crude protein 79.87 81.34 79.79 ^b 83.83 ^{a a, b} Different subscripts on the same line have significant differences at p <0.05. (Han et al. 2000b)

In addition, feed additives for gastrointestinal function, which are mainly composed of hydrochloric acid, betaine and pepsin, increase protein digestibility and also increase the digestibility of amino acid, which is a constituent of protein, according to Han et al. (2000b). The addition of food increases protein and dry matter digestibility, as well as ileal and digestive digestibility of valine, isoleucine, leucine and phenylalanine (Table 8). In conclusion, it was proved by Table 6, 7, 8 that the feed additive for gastrointestinal function, which is mainly composed of hydrochloric acid, betaine and protease, is effective in the digestion of nutrients in rats.

Effects of Feeding Additives for Gastrointestinal Functions on the Digestibility of Aileum and Fractional Amino Acids in Weaning Pigs Digestion rate (%) Ileum digestibility (%) Control Test Control Test Valine 85.20 ^a 89.28 ^b 82.34 94.01 Isoleucine 85.96 ^a 91.18 ^b 81.60 87.82 Leucine 87.42 ^a 91.39 ^b 84.31 ^a 88.95 ^b Phenylalanine 85.04 ^a 93.87 ^b 85.55 88.36 Azinin 93.97 94.39 91.33 ^a 96.81 ^{b a, b} Different subscripts on the same line have significant differences at p <0.05. (Han et al. 2000b)

3) Effect on blood properties

The effects of gastrointestinal stimulating feed additives based on hydrochloric acid, betaine and protease are shown in Table 9. As shown in Table 9, the number of leukocytes and globulin in blood of rats fed the feed additives for gastrointestinal function showed a tendency to increase considerably compared to the control, and these dietary supplements raised immunity to disease in young rats. It seems certain to give. However, there was no difference in the calcium and phosphorus contents between the test and control groups. The important physiological significance of these experiments is that blood properties, expressed in white blood cells, red blood cells, total proteins, albumin, globulin, calcium, phosphorus and magnesium, provide scientific criteria for determining the health of animals.

In general, the albumin and globulin content of the blood protein accounts for about 60 and 40%, respectively, but the protein and energy deficiency, or the immune response occurs, the percentage of albumin and globulin in the blood protein changes. That is, when protein deficiency occurs, blood protein content and albumin content decrease simultaneously, and when an immune reaction occurs, albumin content decreases and globulin content increases (Jahoor et al., 1999). In this study, normal nutrient supply was achieved, and certain pathogens and immunogens were not injected to generate an immune response. In other words, the increase in the globulin content and the white blood cell count in the rats fed the feed containing the gastrointestinal stimulating feed additives under normal nutrition and health conditions is an important immunological phenomenon. Given the fact that little has been done on the effects of gastric acid enhancers on blood albumin and globulin levels, the results of this study are the first to suggest that gastrointestinal supplements are very important as dietary supplements. There is no way.

Effect of Gastrointestinal Feed Additives on Blood Characteristics in Rats Treatment Leukocytes (10 ³ / μl) Erythrocytes (10 ⁶ / μl) Protein (g / μl) Albumin (g / μl) Globulin (g / μl) Calcium (mg / ㎗) Phosphorus (mg / dl) Magnesium (mg / ㎗) Control 11.33 7.38 5.73 3.03 ^a 2.70 11.73 7.03 1.90 Test 13.56 7.63 5.70 2.93 ^b 2.77 11.87 7.33 1.93 Standard error 0.67 0.22 0.07 0.03 0.05 0.16 0.32 0.03 ^{a, b} Different subscripts on the same line have significant differences at p <0.05. (Han et al. 2000a)

4) Effect on organ weight and intestinal microflora

Table 10 below shows that the stomach weight, small intestine length and weight are slightly reduced, but the liver weight is about 30% heavier in rats fed gastrointestinal feed additives containing hydrochloric acid, betaine and pepsin. have. This phenomenon is a very important physiological discovery for the health of animals.

In general, gastric acid feed may affect microorganisms in the stomach and in the upper part of the small intestine. That is, they inhibit the growth of harmful microorganisms, such as E. coli (Kirchgessner et al., 1992). This effect was also confirmed in this study. As shown in Table 10, when the feed containing the gastrointestinal feed additive was fed, the total number of microorganisms in the colon and the number of Escherichia coli were slightly reduced.

Effect of Gastrointestinal Feed Additives on Organ Weight and Intestinal Microflora in Rats Treatment Stomach (g) Liver (g) Pancreas (g) Intestine microbe Length (cm) Weight (g) Enteric microorganisms (log ₁₀ CFU / mL) E. Coli (log ₁₀ CFU / mL) Control 1.46 12.03 0.73 115.3 12.13 ^a 2.35 ^a 2.14 Test 1.27 15.43 0.77 99.7 11.33 ^b 1.30 ^b 2.00 Standard error 0.09 0.98 0.04 4.90 0.23 0.25 0.17 ^{a, b} Different subscripts on the same line have significant differences at p <0.05. (Han et al. 2000a)

In addition, according to a recent study by Han et al. (1999, 2000b), feeding a powder that promotes gastrointestinal function increases acidity in the intestine of young piglets, increases protein digestion or ileal digestibility, and is completely metabolized in the body. It has also been proven that by increasing the supply effect, the growth rate of young animals can be greatly improved (Tables 11 and 12). In addition, feed additives for promoting gastrointestinal function have a good effect on the colonization of intestinal microflora. Han et al. (1999) conducted a study with piglets weighing 4.2 kg, which was the reason for the premature birth. According to the study, E. coli causes a large increase in the number of lactobacilli, a beneficial microorganism in the small intestine, and the cause of diarrhea. The number of is significantly reduced. On the other hand, the increase in the number of lactobacilli in the colon was small, but the number of Escherichia coli was slightly decreased (Table 12). In the case of people ingesting the powder is expected to be effective in preventing various diseases caused by lack of gastric acid secretion. This will be an example of scientific proof that gastric acid supplements are health supplements that protect the health of the digestive system of animals by inhibiting the growth of harmful microorganisms in the intestine.

Effect of Gastrointestinal Feed Additives on Growth Capacity, Dry Matter and Protein Digestibility of Weaning Pigs 0-14 days after weaning 0-21 days after weaning Control Feed additive for gastrointestinal function Control Feed additive for gastrointestinal function Growth capacity Daily gain (g) 177 211 239 268 Feed efficiency 1.69 ^a 1.30 ^b 1.60 ^a 1.36 ^b digestibility building(%) 79.94 ^a 83.96 ^a 77.94 ^b 82.96 ^a Crude Protein (%) 79.87 81.34 79.79 ^b 83.83 ^{a a, b} Significant at p <0.05 level between different subscripts. (Han et al. 2000b)

Effect of Gastrointestinal Feed Additives on Gastric pH, Growth, Digestion Rate and Intestinal Microflora in Stomach Control Gastrointestinal Promoting Feed Additives Control Gastrointestinal Promoting Feed Additives PH in the stomach 3.88 3.50 Chairman's digestion rate (%) Start weight (kg) 4.16 4.21 building 73.32 78.32 End weight (kg) 11.02 11.55 Crude protein 75.40 81.05 Feed Intake (g / day) 291 ^A 315 ^B Small intestine microflora (CFU ^* / ml) Daily weight gain (g / day) 221 237 Lactobasilai 8 12 Feed efficiency 1.32 1.33 Escherichia coli 12 10 Digestion rate (%) Colonic microflora (CFU / mL) building 82.87 84.07 Lactobasilai 23 26 Crude protein 86.89 ^a 87.59 ^b Escherichia coli 21 19 ^{A, B} Significant at p <0.01 level between different subscripts. (H et al., 1999) ^{a, b} Significant at p <0.05 level between different subscripts. ^* CFU: Abbreviation for colony forming unit.

5) Effect on enzyme activity in the small intestine

The effects of gastrointestinal stimulating feed additive, a type of dietary supplement containing hydrochloric acid, betaine and proteolytic enzymes, on the carbohydrate degrading enzymes in the small intestine of rats were investigated. Carbohydrate degrading enzymes, lactase, maltase, and sucrase, respectively, in rats fed the feed, increased 128, 46, and 156%, respectively, compared to the control. In this study, the energy digestibility of the test group was increased compared to the control group, because the dietary supplement for gastrointestinal function promoted the activity of carbohydrate degrading enzyme in the small intestine.

Effect of Gastrointestinal Feed Additives on Carbohydrate Degrading Enzymes in Rats Treatment Lactase (unit * / g protein) Maltase (unit / g protein) Sucrase (unit / g protein) Control 10.89 ^a 60.68 ^a 10.02 ^a Test 24.83 ^b 88.51 ^b 25.64 ^b Standard error 3.36 6.43 3.54 * unit: μmol of substrate decomposed per minute (Han et al., 2000a) ^{a, b} Different subscripts in the same row differed significantly at p <0.05.

6) Effect on the intestinal villi height and the depth of the vortex

The effects of villi height, villi and depth in the small intestine were examined and the results are shown in Table 14. As can be seen in Table 14, the height of duodenal villi increased significantly compared to the control by the supply of gastric acid and protease (p <0.05). In addition, the feed additives for promoting gastrointestinal function also influenced the depth of the vortex. The depth of the ileum in the ileum of rats fed the diet supplemented with the test healthy food was shorter than the control group (p <0.05). It was also found in the duodenum area. In growing animals, the development of intestinal villi and the depth and shape of the vortex are of great importance. The longer the villi, the longer the absorption area of the small intestine, the higher the absorption capacity of nutrients, and the intestinal digestive enzyme titer is affected by the morphological changes of the villi and villi. As can be seen in Table 14, as a result of feeding the feed additive for gastrointestinal function promotion of the present invention, the titer of carbohydrate degrading enzyme was found to be much higher than that of the control.

Effect of Gastrointestinal Feed Additives on the Intestinal Villi Height, Melting and Depth in Rats Villi Height (μm) Melting depth (μm) Duodenum factory Chairman Duodenum factory Chairman Control 456.04 ^a 636.56 554.58 261.08 194.19 280.08 ^a Test 843.60 ^b 598.56 511.92 241.08 194.28 187.76 ^b Standard error 87.03 19.49 14.04 8.30 7.93 22.41 ^{a, b} Different subscripts on the same line have significant differences at p <0.05. (Han et al. 2000a)

According to the test results of the present invention, the rats supplied with the gastrointestinal stimulation supplement containing mainly hydrochloric acid / betaine and pepsin, the protease, are limited to greatly improving the growth ability by improving the digestion and absorption functions of the protein. I don't think so. In other words, by feeding feed additives for promoting gastrointestinal function in growing animals, digestion of protein is increased by increasing acidity in the stomach. It turns out that they can improve their abilities. In addition, the number of Escherichia coli in the colon and reduced immune function in the blood can improve the health of the animal as a whole. This effect improved the growth rate and feed efficiency of the animals.

In conclusion, feed additives for the promotion of gastrointestinal function with proteolytic enzymes were found to increase gastric acidity, accelerate intestinal function development, and the like in growing animals and animals suffering from certain diseases or whose gastric acid secretion function decreased due to age. Due to the increased titer of digestive enzymes and the reduction of harmful microorganisms, including E. coli, it can be said that it has an excellent effect in improving the health condition.

The present invention is not limited to the technical spirit of the above-described specific embodiments or diagrams, and any person of ordinary skill in the art without departing from the gist of the present invention claimed in the claims. Various modifications are possible, of course, and such changes are within the scope of the claims.

By taking a feed additive for gastrointestinal function, which is mainly composed of hydrochloric acid, betaine and protease, which is the composition of the present invention, it is excellent in physiological problems that may be caused by gastric acid secretion caused by certain diseases such as disorder, aging or dementia. Effect appeared.

To illustrate the specific effects, as described above, as shown in the experiment on growth ability, the rats and piglets were significantly improved as a result of ingesting the feed supplemented with the gastrointestinal feed additive. In the digestibility of various nutrients, the digestibility of dry matter, crude protein and certain amino acids (valine, isoleucine, leucine, phenylalanine, azinin) was greatly improved.

In addition, as a result of increased white blood cell count and globulin content, an excellent effect on immunity was demonstrated.

In addition to the effects described above, in the intestinal villi height, the depth of the vortex and the digestive enzyme titer, the villi height and enzyme titer of rats fed the feed supplemented with gastrointestinal stimulator were significantly improved compared to the control, and In the rats fed the feed supplemented with gastric acid enhancer has an excellent effect of reducing the number of E. coli and total microorganisms.

Claims (2)

A feed additive for the promotion of gastrointestinal function, which contains 0.1 to 0.2% by weight of hydrochloric acid, betaine and pepsin, a protease, per day. The method of claim 1, The feed additive is 520 mg / g of hydrochloric acid and betaine, pepsin is a protease 200 mg / g and the excipient (carrier) comprises a feed additive for gastrointestinal function.