KR100377921B1 - 엑시머레이저 각막절제술에서의 안연고 제조방법 - Google Patents
엑시머레이저 각막절제술에서의 안연고 제조방법 Download PDFInfo
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- KR100377921B1 KR100377921B1 KR10-2000-0023862A KR20000023862A KR100377921B1 KR 100377921 B1 KR100377921 B1 KR 100377921B1 KR 20000023862 A KR20000023862 A KR 20000023862A KR 100377921 B1 KR100377921 B1 KR 100377921B1
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- amnion
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- amniotic membrane
- corneal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0048—Eye, e.g. artificial tears
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
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- Ophthalmology & Optometry (AREA)
- Pregnancy & Childbirth (AREA)
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Abstract
Description
Rabbits | OD(AM oint group) | sum | 평균 | p | ||||
1 | 1 | 1 | 1 | 1 | 1 | 5 | 1.00 | |
2 | 6 | 6 | 9 | 1 | 1 | 23 | 4.60 | |
3 | 3 | 2 | 2 | 1 | 2 | 10 | 2.00 | |
4 | 1 | 1 | 1 | 2 | 1 | 6 | 1.20 | |
5 | 3 | 1 | 1 | 2 | 1 | 8 | 2.00 | |
6 | 3 | 5 | 1 | 2 | 4 | 15 | 3.00 | |
7 | 2 | 2 | 1 | 3 | 1 | 9 | 1.80 | |
8 | 1 | 2 | 3 | 2 | 1 | 9 | 1.80 | |
9 | 3 | 4 | 3 | 2 | 2 | 14 | 2.80 | |
10 | 8 | 2 | 2 | 2 | 2 | 16 | 3.20 | |
2.34 | 0.000116 |
OS(Control) | 평균 | ||||||
1 | 4 | 8 | 9 | 10 | 12 | 43 | 8.60 |
2 | 22 | 11 | 23 | 23 | 28 | 107 | 21.40 |
3 | 10 | 12 | 8 | 7 | 5 | 42 | 8.40 |
4 | 2 | 1 | 8 | 7 | 4 | 22 | 4.40 |
5 | 12 | 9 | 7 | 13 | 7 | 48 | 9.60 |
6 | 3 | 9 | 17 | 16 | 3 | 48 | 9.60 |
7 | 18 | 17 | 6 | 7 | 13 | 61 | 12.20 |
8 | 12 | 3 | 5 | 7 | 15 | 42 | 8.40 |
9 | 18 | 16 | 12 | 5 | 17 | 68 | 13.60 |
10 | 13 | 5 | 4 | 7 | 6 | 35 | 7.00 |
10.32 |
변수 1 | 변수 2 | |
평균 | 11.5 | 51.6 |
분산 | 30.0556 | 540.2667 |
관측수 | 10 | 10 |
피어슨 상관 계수 | 0.72982 | |
가설 평균차 | 0 | |
자유도 | 9 | |
t 통계량 | -6.4684 | |
P(T<=t) 단측 검정 | 5.8E-05 | |
t 기각치 단측 검정 | 1.83311 | |
P(T<=t) 양측 검정 | 0.00012 | |
t 기각치 양측 검정 | 2.26216 |
Rabbits | OD(AM oint group) | sum | 평균 | p | ||||
1 | 32 | 53 | 78 | 44 | 27 | 234 | 46.8 | |
2 | 15 | 17 | 21 | 28 | 27 | 108 | 21.6 | |
3 | 14 | 27 | 30 | 19 | 25 | 115 | 23 | |
4 | 45 | 48 | 62 | 34 | 85 | 274 | 54.8 | |
5 | 17 | 16 | 16 | 14 | 29 | 92 | 18.4 | |
6 | 24 | 27 | 33 | 24 | 27 | 135 | 27 | |
7 | 42 | 94 | 60 | 78 | 77 | 351 | 70.2 | |
8 | 8 | 7 | 9 | 8 | 5 | 37 | 7.4 | |
9 | 14 | 15 | 15 | 17 | 12 | 73 | 14.6 | |
10 | 54 | 48 | 66 | 78 | 40 | 286 | 57.2 | |
34.1 | 0.003578949 |
OS(Control) | 평균 | ||||||
1 | 58 | 77 | 88 | 97 | 100 | 420 | 84 |
2 | 87 | 44 | 58 | 98 | 87 | 374 | 74.8 |
3 | 27 | 29 | 44 | 22 | 21 | 143 | 28.6 |
4 | 77 | 95 | 85 | 121 | 88 | 466 | 93.2 |
5 | 18 | 21 | 27 | 45 | 13 | 124 | 24.8 |
6 | 52 | 23 | 30 | 31 | 37 | 173 | 34.6 |
7 | 122 | 115 | 38 | 44 | 52 | 371 | 74.2 |
8 | 42 | 17 | 13 | 22 | 27 | 121 | 24.2 |
9 | 38 | 42 | 41 | 35 | 27 | 183 | 36.6 |
10 | 37 | 55 | 61 | 87 | 131 | 371 | 74.2 |
54.92 |
변수 1 | 변수 2 | |
평균 | 170.5 | 274.6 |
분산 | 11378.05556 | 18727.4 |
관측수 | 10 | 10 |
피어슨 상관 계수 | 0.788075766 | |
가설 평균차 | 0 | |
자유도 | 9 | |
t 통계량 | -3.907387395 | |
P(T<=t) 단측 검정 | 0.001789475 | |
t 기각치 단측 검정 | 1.833113856 | |
P(T<=t) 양측 검정 | 0.003578949 | |
t 기각치 양측 검정 | 2.262158887 |
Claims (2)
- 삭제
- 양막을 얻기 위해 태반을 lamina flow hood 내에서 항생제가 첨가된 sterile PBS로 세척하는 단계와,양막(amnion)을 융모막(chorion)으로부터 박리하고 nitrocellulose paper에 양막의 basement membrane이 위로(stroma side down) 가도록 펴서 부착시키는 단계와,DMEM(Dulbecco-modified Eagle medium)과 glycerol이 1:1로 혼합된 용액에 넣어 -80'C에 보관하는 단계와,상기 보관된 양막을 -80'C 냉동실에서 꺼내 실온에서 녹인 후 nitrocellulose paper에서 양막을 떼어내고 묻어 있는 배양액을 생리식염수로 세척하는 단계와,양막의 무게를 측정한 후 조직 마쇄기에 넣고 잘게 분쇄시킨 후 동결건조기에서 건조시키는 단계와,5 ㎛ 이하만 여과되는 필터에 양막을 통과시킨 후 멸균하는 단계와,바셀린 75중량%, 파라핀 15중량%, 라놀린 5중량%에 양막분말 5중량%을 첨가하는 단계와,안구표면에 잘 퍼지게 하기 위해 융점 35℃로 맞추어 제조하는 단계를 포함하여 이루어지는 것을 특징으로 하는 안연고 제조방법.
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KR10-2000-0023862A KR100377921B1 (ko) | 2000-05-04 | 2000-05-04 | 엑시머레이저 각막절제술에서의 안연고 제조방법 |
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KR10-2000-0023862A KR100377921B1 (ko) | 2000-05-04 | 2000-05-04 | 엑시머레이저 각막절제술에서의 안연고 제조방법 |
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KR20010100588A KR20010100588A (ko) | 2001-11-14 |
KR100377921B1 true KR100377921B1 (ko) | 2003-03-29 |
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Families Citing this family (15)
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AU2003272536A1 (en) * | 2002-09-18 | 2004-04-08 | Emiliano Ghinelli | Use of a human amniotic membrane composition for prophylaxis and treatment of diseases and conditions of the eye and skin |
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US9180145B2 (en) | 2012-10-12 | 2015-11-10 | Mimedx Group, Inc. | Compositions and methods for recruiting and localizing stem cells |
US8946163B2 (en) | 2012-11-19 | 2015-02-03 | Mimedx Group, Inc. | Cross-linked collagen comprising metallic anticancer agents |
US9155799B2 (en) | 2012-11-19 | 2015-10-13 | Mimedx Group, Inc. | Cross-linked collagen with at least one bound antimicrobial agent for in vivo release of the agent |
US10206977B1 (en) | 2013-01-18 | 2019-02-19 | Mimedx Group, Inc. | Isolated placental stem cell recruiting factors |
EP2945639B1 (en) | 2013-01-18 | 2020-09-30 | MIMEDX Group Inc. | Methods for treating cardiac conditions |
US10029030B2 (en) | 2013-03-15 | 2018-07-24 | Mimedx Group, Inc. | Molded placental tissue compositions and methods of making and using the same |
US9446142B2 (en) | 2013-05-28 | 2016-09-20 | Mimedx Group, Inc. | Polymer chelator conjugates |
EP3094336A4 (en) | 2014-01-17 | 2018-02-14 | MIMEDX Group Inc. | Method for inducing angiogenesis |
AU2015308795B2 (en) | 2014-08-28 | 2019-05-23 | Mimedx Group, Inc. | Collagen reinforced tissue grafts |
US9603967B2 (en) | 2015-05-08 | 2017-03-28 | Vivex Biomedical, Inc. | Placental tissue assembly |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR940003751A (ko) * | 1992-08-07 | 1994-03-12 | 이보영 | 흑색 비닐 레쟈를 표지로 한 장부의 인쇄방법 |
KR20010098716A (ko) * | 2000-04-19 | 2001-11-08 | 김재찬 | 안구표면질환 치료용 양막 추출물 |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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KR940003751A (ko) * | 1992-08-07 | 1994-03-12 | 이보영 | 흑색 비닐 레쟈를 표지로 한 장부의 인쇄방법 |
KR20010098716A (ko) * | 2000-04-19 | 2001-11-08 | 김재찬 | 안구표면질환 치료용 양막 추출물 |
Non-Patent Citations (2)
Title |
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논문1999. 12 * |
논문1999. 2 * |
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