KR100371560B1 - Dna detector - Google Patents

Abstract

Start the gene reader. The gene reader includes a light source operated by the power supply. The light emitted from the light source portion is removed from the portion other than the desired wavelength band while passing through the incident light adjusting portion. Light passing through the incident light control unit is obliquely incident on at least one gene chip adhered to the slide and coated with a fluorescent material. The slide is position controlled by a transfer mechanism operated automatically or manually. The light emitted by the fluorescent material is removed through the reflected light control unit except for the part corresponding to the emission wavelength of the fluorescent material. Light passing through the reflected light control unit is processed into an image by a CCD camera. This image is read by the image processing reader, whereby the user diagnoses the disease. The CCD camera photographs any one inspection area several times, and the image processing reading unit superimposes and reads several images.

Description

Gene Reader {DNA DETECTOR}

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a gene reader, and more particularly, to a genetic plate reader for reading and analyzing a DNA chip, which is a human tissue sample, for diagnosing a human disease.

In recent medical fields, disease diagnosis using an in-vitro diagnosis system is rapidly spreading. The in vitro diagnostic system refers to a diagnosis of various human diseases or genetic diseases by reading and analyzing a tissue sample obtained from a human body, that is, a gene chip. For the purpose of reading and analyzing such a gene chip, a gene reader having a scanner that scans light into the gene chip and quantifies the light reflected from the gene chip is used.

Meanwhile, when analyzing a gene chip using a scanner, a fluorescent probe is used as an analysis medium. Fluorescent material is a material that emits light of a specific wavelength longer than the scanned wavelength when the light of a particular wavelength is scanned. Using these characteristics, if a fluorescent material having different optical properties is used, various materials can be examined at the same time. In order to scan light of a specific wavelength with a fluorescent material, a suitable light source and an optical filter for selecting only light of a desired wavelength are required. In addition, in order to detect the light emitted from the fluorescent material, another optical filter that collects the emitted light and selects only the light belonging to the emission wavelength of the fluorescent material from the collected light should be used. Should be quantified.

Scanners with this function use high-density polychromatic phosphors to analyze many genetic diseases from the genetic chip to be read at once, so lasers are mostly used as light sources to increase sensitivity and resolution. Photo-multiplier tube (PMT) is used. Since the laser is used as a point light source method, the resolution can be up to 5 to 10 μm, and the photosensitivity of the light magnifier can be up to 1 molecule / μm ² .

In order to scan the entire area of a general slide having a width of 25 mm and a length of 75 mm within 5 minutes, an apparatus capable of transferring a light source or a slide in a short time is employed in the scanner. As a method of transporting the light source, a method of controlling the mirror located in the path through which the laser passes at a high speed to move the laser beam in a zigzag form on the slide surface is used. On the other hand, a dedicated X-Y stage is mostly used for the method of moving a slide.

Meanwhile, in order to remove background noise as much as possible from the fluorescence emitted from the gene chip dotting on the slide surface, most scanners use a confocal method. The confocal method is a method in which a very small pin hole is placed in the path of light so that only light that is scanned at a desired distance can pass.

A schematic configuration of a general gene reader using this confocal method is shown in FIGS. 1 and 2. First, referring to FIG. 1, a laser beam emitted from a laser light source (not shown) is passed through a band-pass filter (1: band-pass filter) for passing only a specific wavelength thereof by a beam splitter (2). Is switched 90 °. Subsequently, the laser beam is incident on the slide 3 to which the gene chip is attached, and as shown, the slide 3 is out of the focal position of the laser beam. The light emitted by the fluorescent material of the gene chip is collected by the objective lens 4, and then its path is again converted by 90 degrees by the other mirror 5. Then, light passes through another bandpass filter 6 through which only light corresponding to the emission wavelength of the fluorescent material passes, and then enters the confocal membrane 8 through the sensing lens 7. At this time, as described above, since the light is out of the focus position, the light does not pass through the pin hole 8a of the confocal membrane 8.

On the other hand, as shown in FIG. 2, if the laser beam is incident on the slide 3 at the focal position precisely, the light collected through the sensing lens 6 will pass through the pinhole 8a of the confocal membrane 8 accurately. Thus, it is possible to enter the sensing unit 9. At this time, for the scanning of the entire gene chip adhered to the slide 3, the movement of the light source or the slide 3 in zigzag is precisely controlled.

The signals recognized by the detector 9 are converted into an image by their processing units and stored. At this time, since these images are displayed as dots of very small size every moment, independent image processing software which collects, processes and reads all of these images after completion of scanning of all the light over the entire inspection area is required.

By the way, the conventional gene reader is a very expensive equipment for the optical magnification tube used as the detector 9, and also has many implementations of a moving mechanism for scanning several points of light into the entire area of the slide 3 within approximately 5 minutes. Due to the cost, its price is too high. In other words, conventional readers require the expensive components described above because they must diagnose almost all diseases, not just a specific disease diagnosis, but there is no need to use a conventionally expensive reader for a recently presented disease diagnosis gene chip. Many are pointed out.

In addition, the conventional reader has the disadvantage that the overall configuration is very complicated due to the mechanism for the high-speed scanning method.

In addition, since a conventional reader is an optical device that requires a high degree of precision, it is difficult to control fine precision movement, and in particular, a professional technician is required for fine adjustment of the processed image after the movement.

A conventional reader (US Pat. No. 6,140,653) is shown in FIG. 3, which has been proposed to overcome several disadvantages of the gene reader with such a laser scanning scanner. The reader shown in FIG. 3 uses a lamp 200 instead of a laser as a light source and a CCD camera 180 which is cheaper than a light augmentation tube as a detector.

A lamp 200 arranged to scan light in a direction parallel to the surface of the stage 140 on which the slide 120 is placed, ie, horizontally, is surrounded by the reflector 220. In front of the lamp 200, a heat dissipation mirror 240 for removing high heat of light and a filter 260 that transmits only a selected frequency band are sequentially disposed. The light passing through the filter 260 is reflected upward by the flat mirror 340 disposed obliquely, and then is incident obliquely onto the slide 120 by a concave mirror 320.

The light emitted from the fluorescent material on the slide 120 passes through the pair of lenses 380 and 400 and the filter 420 disposed therebetween, and then enters the CCD camera 180, whereby the fluorescent image is captured by the CCD camera 180. Is generated. The fluorescence image is read by the image processing reading unit 500, so that it is possible to confirm whether or not the disease is abnormal.

By the way, since the reader shown in Fig. 3 uses a CCD camera instead of an optical tube as a detector, there is an advantage in that the scanner price is somewhat lowered. However, a concave mirror is used as a means for inclining the horizontal light emitted from the lamp onto the slide in an inclined manner, and since the concave mirror is very expensive, there is no significant advantage compared to the laser scanning method in terms of price. The reason for the high price of the concave mirror is due to the cost required to precisely manufacture its curvature in accordance with the incident angle.

Therefore, an object of the present invention is to use a lamp or a laser as a light source, use a low-cost CCD camera as a detection means, and in particular, by reducing the number of parts, it is largely dedicated to gene chips only for the diagnosis of specific diseases that are currently on the market. To provide a low-cost gene reader that can be used at no cost.

Another object of the present invention is to enable a disease readout by viewing a video output of a CCD camera by a non-professional general user without a dedicated image processing software.

1 and 2 are schematic configuration diagrams of a general scanner.

3 is a block diagram of a conventional gene reader.

4 is a block diagram of a gene reader according to the present invention.

5 is a block diagram of a reader having a lamp as a light source according to Embodiment 1 of the present invention;

6 is a schematic diagram of a reader having a halogen lamp.

7 is a schematic diagram of a reader having a xenon or mercury lamp.

8 is a configuration diagram of a reader having a light emitting diode portion.

Fig. 9 is a block diagram of a reader having a laser beam as a light source according to Embodiment 2 of the present invention.

10 is a block diagram of a reader that is a linear scan control method.

11 is a timing chart showing a control method of a CCD camera by a linear scan control method.

12 is a block diagram of a reader in which the scanning method is a completely internal reflection method.

13 is an operation explanatory diagram showing an operation of changing the path of a laser beam by a prism;

14 is an operation explanatory diagram showing a phenomenon in which a laser beam is fully internally reflected on a slide;

-Explanation of symbols for the main parts of the drawing-

11; Power supply section 12; Light source

13; Incident light control unit 14; slide

15; Inspection area 16; Reflective Light Control Unit

17; CCD camera 18; Image processing reader

19; Transfer mechanism

In order to achieve the above object of the present invention, the gene reader according to the present invention includes a light source unit operated by a power supply unit. The light emitted from the light source portion is removed from the portion other than the desired wavelength band while passing through the incident light adjusting portion. Light passing through the incident light control unit is obliquely incident on at least one gene chip adhered to the slide and coated with a fluorescent material. The slide is position controlled by a transfer mechanism operated automatically or manually.

The light emitted by the fluorescent material is removed through the reflected light control unit except for the part corresponding to the emission wavelength of the fluorescent material. Light passing through the reflected light control unit is processed into an image by a CCD camera. This image is read by the image processing reader, whereby the user diagnoses the disease. The CCD camera photographs any one inspection area several times, and the image processing reading unit superimposes and reads several images.

On the other hand, a lamp or a laser beam may be used as the light source. First, when a lamp is used, the light source unit comprises a lamp. The incident light adjusting unit includes a light adjusting lens for adjusting the amount of light and a focal length, a light filter for selectively passing only light of a specific wavelength, and a light reflector for inclining the path of the light to slide. The reflected light control unit includes a light filter for passing only the light corresponding to the emission wavelength of the fluorescent material, and a light control lens for concentrating the light passing through the light filter.

If a halogen lamp is used as the lamp, the halogen lamp is surrounded by the collector. The halogen lamps are arranged at an inclined angle with respect to the surface of the slide. The heat dissipation part, the light control lens, and the light filter are sequentially disposed on the front surface of the halogen lamp. Since a halogen lamp generates a lot of heat, a heat radiating part is adopted.

When a xenon or mercury lamp is used as the lamp, a constant voltage maintenance power supply is used to extend the lamp life. Xenon or Mercury lamps are equipped with a semi-elliptic light reflector as a light collector for concentrating light. A xenon or mercury lamp is also arranged in the same direction as a halogen lamp, and a light filter and a light adjusting lens are sequentially disposed in front of the xenon or mercury lamp.

When a light emitting diode is used as the lamp, the light emitting diode is also arranged to have a predetermined acute angle with the surface of the slide. The light filter and the light adjusting lens are sequentially disposed on the front surface of the light emitting diode in a state inclined at the same inclination angle as that of the light emitting diode.

When the light source is a laser beam, the light source portion includes a laser beam scanning portion and a light deformation portion that transforms the laser beam into a suitable shape. However, when the laser beam is completely reflected inside the slide, since the laser beam must be generated in the form of a dot, the optical deformation part is omitted. The incident light control unit includes a light control lens unit for controlling the path of the laser beam, a light reflector for switching the path of the laser beam, and a rotation drive unit for rotating the light reflector or the light control lens unit. On the other hand, the configuration of the reflected light adjusting unit and the photodetecting unit is the same as before.

The light deformation portion includes a collimating lens that focuses the laser beam, and a cylindrical lens that linearly transforms the laser beam. In addition, when a fully internal reflection method in which the optical deformation part is omitted, the light adjusting lens part includes a prism for switching the path of the laser beam, and a cylindrical lens for adjusting the focus of the laser beam.

With the above configuration, the light emitted from the light source passes through the incident light control unit, and the light of the selected wavelength band passes through the light, and the light is obliquely incident on the fluorescent material applied to the gene chip. The light emitted from the fluorescent material is filtered only by the reflected light control unit corresponding to the emission wavelength of the fluorescent material, and the light is imaged several times by the CCD camera. This superimposed image is input to the image processing reading unit, so that the user can diagnose the disease.

Next, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

4 is a block diagram of a gene reader according to the present invention.

As shown in FIG. 4, the light source unit 12 receives light from the power source unit 11 to emit light. The incident light control unit 13 to which the light emitted from the light source unit 12 is incident removes light having a wavelength other than the desired wavelength band. Light having only a desired wavelength is incident on the inspection area 15 of the slide 14 at a predetermined acute angle, about 45 °. Of course, a gene chip coated with a fluorescent substance is adhered in the test area 15.

On the other hand, the position of the slide 14 is controlled by the transfer mechanism 19, for example, the X-Y axis stage which is a rack / pinion system or a ball screw system. Here, the number of gene chips on the slide 14 is about 1 to 8, and the size of each gene chip is within 1 cm 2. Since the area in which light is scanned into the inspection area 15 is about 1 cm 2, one inspection area 15 can be read by one scanning operation. Therefore, when the reading of one inspection area 15 is completed, another neighboring inspection area 15 may be arranged at the scanning position. Therefore, it is not necessary to precisely control the transfer mechanism as in the prior art. Therefore, you may use the conveyance mechanism 19 which is simply operated manually.

The light is emitted again from the incident fluorescent material, and only the light corresponding to the emission wavelength of the fluorescent material is filtered by the reflected light adjusting unit 16. The filtered light is converted into an image by the CCD camera 17. The CCD camera 17 photographs one inspection area 15 several times. The superimposed image is analyzed and read by the image processing reader 18, and the user views the image to diagnose the disease.

The image processing reading unit 18 processes the image obtained by the light detecting unit 17 in two ways as follows in order to acquire a clearer image. In case an image obtained by the photodetector 17 is not clear, an image summing method which detects each of a predetermined number of times and adds them all together, and removes parts outside the human vision range and noise parts. In order to do this, a banding thresholding method is used.

Here, the incident light and the reflected light are not parallel as in the prior art, and the reason why the incident light is incident on the slide 14 is as follows. If the incident light and the reflected light are parallel, the image quality is excellent because the light intensity is high. However, because the intensity is high, various wavelengths of light are incident directly on the CCD camera 17, and rather it is difficult to obtain a desired image.

Hereinafter, the detailed components of each part that changes according to the type of light source will be described in detail.

Example 1

5 is a block diagram of a reader having a lamp as a light source according to Embodiment 1 of the present invention, FIG. 6 is a block diagram of a reader having a halogen lamp, and FIG. 7 is a block diagram of a reader having a xenon or mercury lamp. 8 is a block diagram of a reader having a light emitting diode.

In the case of having the lamp as a light source, detailed components of the respective parts are shown in FIG. 5. As shown in Fig. 5, the light source portion 12 has a lamp 12a as a light source. On the other hand, in Fig. 5, the light source portion 12 condenses the light and the heat condensing portion 12b for condensing light. Also shown as having a heat dissipation portion 12c, the light collecting portion 12b and the heat dissipation portion 12c are selectively used according to the type of the lamp 12a, an optional use example will be described later.

The incident light adjusting unit 13 includes a light adjusting lens 13a for adjusting the amount of light emitted from the lamp 12a and a focal length, and a light filter 13b which is a band pass filter for passing only light of a specific wavelength. On the other hand, the arrangement order of the light adjusting lens 13a and the light filter 13b is not determined in the order as shown in FIG. 5 but is changed according to the type of the lamp 12a.

The reflected light adjusting unit 16 to which light emitted from the fluorescent material is incident is provided with a light filter 16a for passing only light corresponding to the emission wavelength of the fluorescent material, and a light control lens for collecting and concentrating the light passing through the light filter 16a. (16b).

6 shows the configuration of a reader having a halogen lamp 12a-1 as the lamp 12b. As shown, the halogen lamp 12a-1, which is supplied with electricity from the power supply portion 11a, is inclined so as to form a predetermined acute angle with respect to the surface of the slide 14. Around the halogen lamp 12a-1, a hemispherical light reflector 12b-1 is provided as a light collecting part 12b for concentrating light. The light adjusting lens 13a and the light filter 13b are sequentially disposed on the front surface of the halogen lamp 12a-1. The light filter 16a and the light adjusting lens 16b, that is, the reflected light adjusting unit 16, are sequentially disposed on the vertical portion of the slide 14, and the CCD camera 17 is disposed on the upper portion thereof.

On the other hand, although not shown in Figure 6, in order to increase the sharpness of the image photographed by the CCD camera 17, it is preferable that a shielding chamber (not shown) to isolate the upper region of the slide 14 from the outside. By preventing external light from entering the upper region of the slide 14 by the shielding chamber, it is possible to prevent the image sharpness from being lowered due to light interference.

7 is a configuration diagram of a reader to which a xenon or mercury lamp 12a-2 is applied as the lamp 12a. Since the lifetime of the xenon or mercury lamp 12a-2 greatly depends on the stability of the power supply, the constant voltage sustain type power supply 11b is used as the power supply 11. Then, the semi-elliptical light reflector 12b-2 is applied as the light collecting portion. The arrangement direction of the xenon or mercury lamp 12a-2 is the same as the halogen lamp 12a-1 described above.

The other components are the same as the reader configuration shown in FIG. 6 except that the arrangement of the optical filter 13b and the light adjusting lens 13a is different from that of the reader in FIG. That is, the optical filter 13b is disposed in front of the xenon or mercury lamp 12a-2, and the light adjusting lens 13a is disposed thereafter.

8 is a configuration diagram of a reader having a light emitting diode 12a-3 as a lamp 12a. As shown, the optical filter 13b and the light adjusting lens 13a are sequentially disposed on the front surface of the light emitting diode 12a-3 arranged in the same direction as the halogen lamp 12a-1. The other components are the same as the scanners shown in Figs. 6 and 7, and thus will not be repeated here.

On the other hand, the number of diodes used for the light emitting diodes 12a-3 is determined by the amount of light required in the entire system of the reader. Since the types and sizes of the light emitting diodes are many and varied, the arrangement of the diodes in the light emitting diodes 12a-3 combining them also becomes very diverse.

Example 2

9 is a block diagram of a reader having a laser beam as a light source according to Embodiment 2 of the present invention, FIG. 10 is a block diagram of a reader which is a linear scan control method, and FIG. 11 is a CCD camera using a linear scan control method. 12 is a configuration chart of a reader in which the scanning method is a completely internal reflection method, FIG. 13 is an operation explanatory diagram showing an operation of changing the path of a laser beam by a prism, and FIG. The operation explanatory drawing which shows the phenomenon in which the laser beam is fully internally reflected in the slide.

Fig. 9 shows a block diagram of a reader in which a laser beam is employed as a light source instead of a lamp. As shown, the light source portion 12 includes a laser beam scanning portion 12d and a light deformation portion 12e for transforming the laser beam into a suitable shape. Here, the optical deformation part 12e is applied only to the scanner of the linear scan control method, and is a component that is not necessary because the laser beam must be in the point form when the laser beam is completely internally reflected on the slide.

The incident light adjusting unit 13 includes a light adjusting lens unit 13d for controlling the direction of the laser beam, a light reflector 13c for switching the path of the laser beam, and a light reflector 13c or a light adjusting lens unit 13d. It includes a rotation drive unit 13e for rotating. On the other hand, the number and type of the light control lens unit 13d is changed according to the scanning method of the laser beam, of course. The remaining components are the same as the components shown in FIG.

10 is a block diagram showing a reader of a linear scan control method. As shown, the light source unit 12 of this control type scanner has a collimating lens 12e-1 for focusing the laser beam and a cylindrical lens for linearly modifying the focused point-shaped laser beam. It has the optical deformation part 12e containing (12e-2). On the other hand, the laser beam scanning unit (not shown) is arranged in a direction perpendicular to the surface of the slide 14, that is, in a vertical direction.

The light control unit 13 rotates the polygon mirror 13c and the polygon mirror 13c to reflect the laser beam incident vertically downward to be incident on the slide 14 at a predetermined acute angle. It includes a rotational drive (not shown) to continuously change the position. That is, in Fig. 10, the incident position of the laser beam is changed while continuing from the dotted line to the solid line position by the rotating polygon mirror 13c, resulting in one surface shape. In the present embodiment, on the other hand, a hexagon mirror is used as the polygon mirror 13c.

Here, the width of the polygon mirror 13c is equal to the length of the laser beam which is a linear light source. The width of the laser beam is several tens to hundreds of micrometers, but the laser beam incident on the slide 14 by the polygon mirror 13c rotating at a very high speed reciprocates the inspection area at a high speed. Therefore, while obtaining a higher amount of light than the surface light source method with respect to the scanning area, an image almost similar to the surface light source method can be obtained due to the afterimage effect in the CCD camera 17. More specifically, this will be described with reference to FIG. 11. The time that the hexagonal mirror 13c rotates 1/6 is t1. In the meantime, the linear laser beam emitted from the incident light adjusting unit 13 moves from the position x1 to x2 in the inspection area 15 and returns to the position x1 again. Therefore, almost the same effect as scanning all the inspection area 15 by the surface light source method can be obtained.

In addition, since other components are the same as the structure of the other scanner mentioned above, it is not repeated here.

12 is a block diagram of a scanner in which the laser beam incident on the slide is fully internally reflected. As shown, this type of scanner has no light deformation since the laser beam must be incident in the form of dots. That is, the laser beam scanned by the laser beam scanning part 12d disposed orthogonally to the surface of the slide 14 is incident directly to the incident light adjusting part 13 without passing through the light deformation part. The incident light control unit 13 includes a prism 13d-1 for changing the path of the laser beam, a rotation driver 13e which is a direct current motor for rotating the prism 13d-1, and a cylindrical lens for adjusting the focus of the laser beam. 13d-2, and the light reflector 13c which obliquely reflects the laser beam onto the slide 14. The operation of changing the path of the laser beam by the rotating prism 13d-1 is shown in FIG.

The dot-shaped laser beam reflected by the light reflector 13c is scanned obliquely into the slide 14, as shown in FIG. 14, in which the scanning angle is determined to be a fully internal reflection inside the slide 14. As a result, the point-shaped laser beam scanned on the slide 14 appears as one line light source along the scanning direction. Therefore, the linear light source effect as described above can be obtained here as well.

On the other hand, the other components are the same as the configuration of the reader shown in Fig. 10, and will not be repeated here.

As described above, according to the present invention, a low-cost CCD camera is used without using an optical enlargement tube, so that the price of the reader can be lowered, and the cost reduction can be achieved by using a transfer mechanism with a simple structure. In particular, since there are fewer components such as concave lenses than in the related art, there are excellent effects that can significantly reduce the price of the reader in many respects.

In addition, the reader according to the present invention can be easily adjusted by the general user, and also has the advantage that the diagnostic result can be directly confirmed without the help of an expert.

Although the present invention has been described in detail with reference to preferred embodiments, the present invention is not limited to the above embodiments, and various modifications may be made by those skilled in the art within the scope of the technical idea of the present invention. .

Claims (15)

A gene reader used for diagnosing a disease by scanning light onto a slide to which at least one gene chip coated with a fluorescent material is attached as a human tissue sample, and reading the light emitted from the fluorescent material, A light source unit; An incident light control unit configured to pass only light of a specific wavelength band emitting light of a fluorescent material among the light emitted from the light source unit on a slide; A reflected light adjusting unit configured to pass only light corresponding to an emission wavelength of the fluorescent material among the light emitted from the light emitting material; A CCD camera generating a plurality of images of the light passing through the reflected light adjusting unit; An image processing reading unit which receives and superimposes a plurality of output images from the CCD camera and reads the overlapping images; And And a transfer mechanism for moving the slide after the reading result of the image processing readout portion so that another gene chip is in the incidence position for subsequent operation. The gene reader of claim 1, wherein the light source unit is a lamp arranged in a direction in which light is incident on the slide inclinedly. 3. The gene reader of claim 2, wherein the lamp is selected from the group consisting of halogen lamps, xenon lamps, mercury lamps, and light emitting diodes. The gene reader of claim 1, wherein the light source unit is a laser scanning unit disposed orthogonal to the slide surface. A gene reader used for diagnosing a disease by scanning light onto a slide to which at least one gene chip coated with a fluorescent material is attached as a human tissue sample, and reading the light emitted from the fluorescent material, A lamp disposed obliquely at a predetermined acute angle with respect to the slide surface; An incident light adjusting lens controlling an amount of light emitted from the lamp and a focal length; An incident light filter passing only light having a wavelength band for emitting fluorescent material among the light passing through the incident light adjusting lens on a slide; A reflected light filter passing only light corresponding to the emission wavelength of the fluorescent material; A reflection light adjusting lens for collecting and concentrating the light passing through the reflection light filter; A CCD camera producing a plurality of images of the light passing through the reflected light adjusting lens; An image processing reading unit which receives and superimposes a plurality of output images from the CCD camera and reads the overlapping images; And And a transfer mechanism for moving the slide after the reading result of the image processing readout portion so that another gene chip is in the incidence position for subsequent operation. 6. The gene reader of claim 5, wherein the lamp is a halogen lamp surrounded by a light collecting part for condensing light in one direction, and the reflected light filter, the adjusting lens, and the CCD camera are sequentially disposed on the vertical portion of the slide. 6. The gene reader of claim 5, wherein the lamp is a xenon or mercury lamp surrounded by a light collecting part in one direction, and wherein the reflected light filter, the adjusting lens, and the CCD camera are disposed vertically on the slide. 8. The gene reader of claim 7, wherein a power supply for supplying electricity to the xenon or mercury lamp is a constant voltage maintaining type. 6. The gene reader of claim 5, wherein the lamp is a light emitting diode, and wherein the reflected light filter, the adjusting lens and the CCD camera are disposed vertically on the slide. A gene reader used for diagnosing a disease by scanning light onto a slide to which at least one gene chip coated with a fluorescent material is attached as a human tissue sample, and reading the light emitted from the fluorescent material, A laser beam scanning portion orthogonally disposed with respect to the slide surface; An optical deformation unit configured to linearly change the point laser beam scanned by the laser beam scanning unit; A polygonal light reflector that reflects the linear laser beam and is incident obliquely onto a slide; A rotation driver for rotating the polygon light reflector so that the linear laser beam incident on the slide becomes a plane; A reflected light filter passing only light corresponding to the emission wavelength of the fluorescent material; A reflection light adjusting lens for collecting and concentrating the light passing through the optical filter; A CCD camera producing a plurality of images of the light passing through the reflected light adjusting lens; An image processing reading unit which receives and superimposes a plurality of output images from the CCD camera and reads the overlapping images; And And a transfer mechanism for moving the slide after the reading result of the image processing readout portion so that another gene chip is in the incidence position for subsequent operation. The method of claim 10, wherein the light deformation portion A collimating lens for adjusting the focus of the point laser beam; And And a cylindrical lens for linearly changing the point laser beam. 11. The gene reader of claim 10, wherein the polygonal light reflector is hexagonal. A gene reader used for diagnosing a disease by scanning light onto a slide to which at least one gene chip coated with a fluorescent material is attached as a human tissue sample, and reading the light emitted from the fluorescent material, A laser beam scanning unit disposed orthogonal to the surface of the slide; A light adjusting lens unit for changing a path and adjusting a focus of the point laser beam scanned by the laser beam scanning unit; An optical reflector disposed at an angle such that the point laser beam passing through the light adjusting lens unit is inclined to the slide and the point laser beam incident on the slide is completely internally reflected inside the slide; A reflected light filter passing only light corresponding to the emission wavelength of the fluorescent material; A reflection light adjusting lens for collecting and concentrating the light passing through the reflection light filter; A CCD camera producing a plurality of images of the light passing through the reflected light adjusting lens; An image processing reading unit which receives and superimposes a plurality of output images from the CCD camera and reads the overlapping images; And And a transfer mechanism for moving the slide after the reading result of the image processing readout portion so that another gene chip is in the incidence position for subsequent operation. The method of claim 13, wherein the light adjusting lens unit A prism for changing the path of the laser beam; A rotation driver for rotating the freezer; And And a cylindrical lens for adjusting the focus of the laser beam. A gene reader used for diagnosing a disease by scanning light onto a slide to which at least one gene chip coated with a fluorescent material is attached as a human tissue sample, and reading the light emitted from the fluorescent material, A halogen lamp disposed inclined at a predetermined acute angle with respect to the slide surface; A condenser disposed to surround the halogen lamp and condensing light emitted from the halogen lamp in one direction; A heat dissipation unit disposed at a front surface of the halogen lamp and dissipating high heat of the light; An incident light adjusting lens configured to adjust an amount of light passing through the heat radiating unit and a focal length; An incident light filter passing only light having a wavelength band for emitting fluorescent material among the light passing through the incident light adjusting lens on a slide; A reflected light filter disposed on a vertical portion of the slide and configured to pass only light corresponding to an emission wavelength of the fluorescent material; A reflection light adjusting lens for collecting and concentrating the light passing through the reflection light filter; A CCD camera producing a plurality of images of the light passing through the reflected light adjusting lens; A shielding chamber that isolates the upper portion of the slide from the outside to prevent the external light from interfering with the image photographed by the CCD camera, thereby reducing the sharpness; An image processing reading unit which receives and superimposes a plurality of output images from the CCD camera and reads the overlapping images; And And a transfer mechanism for moving the slide after the reading result of the image processing readout portion so that another gene chip is in the incidence position for subsequent operation.