KR100365491B1 - Method for agamogenesis of new thorn-free cultivar in Kalopanax septemlobus Koidz - Google Patents

Abstract

The present invention relates to a silent breeding method and a method for promoting germination of seeds of a new variety of non- silkworms belonging to Kalopanax septemlobus Koidz. The present invention relates to a method for promoting germination of seeds, The present invention also provides a method for promoting germination of a seedling by controlling plant growth regulators and post-seeding treatment. In addition, by using the germination propagation method and seed germination promoting method of the present invention, it is possible to stably obtain a large number of beech trees which are known to have a great difficulty in mass propagation, and in general, the germination rate of seeds is only about 8% .

Description

Method for agonistic breeding of a new breed of hornbeam in a thorn-free cultivar in Kalopanax septemlobus Koidz}

The present invention relates to a novel breeding method and a plant growth method by a shallow-grafting and a close-fitting method of a new variety of non- spindle newborn , which has no visible spots in the leaves , branches and shoots and has a smooth bark, belonging to the Kalopanax septemlobus Koidz. Regulating substances and post-seed treatment.

Kalopanax is also called deciduous broad-leaved arboreous tree in gaedureup, eomnamu, haedongpi belongs to the Araliaceae (araliaceae). The bamboo trees that are scattered throughout the Korean peninsula grow well in the north, northeast, northwest slopes, valleys, or fertile forests between 400 and 110 m above sea level. It is a deciduous broad-leaved arboreous tree with a height of 20m. It is gray and has thorns. The leaves are round and have a diameter of 10 ~ 30cm. They are divided into 5 ~ 9 pieces and are gathered at the ends of the branches. The petiole is 7 ~ 30cm long and the surface is almost hairless. There is a mount. Flowers bloom in July ~ August, yellowish green with a diameter of 5mm, and the fruit has a black spherical shape with a diameter of about 4 ~ 5mm.

The stem of pearl is pale yellowish green or grayish brown and has beautiful gloss. It is mainly used as the finest materials such as utensils, furniture, and musical instruments, and has a pharmacological effect on nourishing tonic and neuralgia (Nakai, Araliaceae, 50, 1992; Daeheejang, etc.), 437-438, 1990). Recently, studies on extraction and separation of various kinds of saponin, lignin and antioxidants have been actively conducted (Shao et al ., Chem. Pharm. Bull. 37 (2): 311-314, 1989; Porzel et al ..., Planta Med 58: ... 481-482, 1992; Lee et al, Kor J. Pharmcogn 26 (2): 122-120, 1995; Journal of Jane Smith, etc., Korea plant resources, 11 (2): 89 -119, 1998). Early spring is called primrose, and its taste and flavor are unique, so it is used as a high-preference wildlife (Short-term pregnancy income species, Forestry Research Institute, 324, 1990). On the other hand, when the bloom itself is in summer, the white flowers cover the trees and have a beautiful shape, leaves and fruit.

The rapidly developing biotechnology-derived mutagenesis technology can be expected to mass-produce a variety of new varieties, but inducing mutations using chemical substances or radioactive isotopes has a very low recurrence rate in the natural state, Only a small fraction of them are mutations in the gene replication process. Especially, most of the mutants that are induced are inhibited in the expression of the trait as a recessive factor, so the mutant adapted to the surrounding environment has a rare value. Therefore, it is widely used for the development of a new plant variety by discovering varieties with new characteristics and inducing the varieties and establishing their mass propagation method.

However, despite the high developmental value of bamboo, development studies on new varieties are insufficient. It is a plant of the genus 1 genus that grows only in Northeast Asia such as Korea, Japan, China, and eastern Russia, and has been named by some scholars as a variant of hairy, There is no report on the Kii trees.

The seedling breeding is a double dormancy seed, which has a germination characteristic of 2 years, and the average germination rate is very low, about 8% in the same year. In case, the survival rate is remarkably lowered as the number of recipients increases, so that it is difficult to proliferate the mutant of the aged tree, and the multiplication by grafting is not performed. Therefore, it is urgent to develop a new breed and to develop a method for mass proliferation of seedlings, such as identification of mutant breeding methods such as excavation, approximation, or grafting, which have completely new traits, and development of germination promoting method of bamboo seeds.

Therefore, the present inventors have conducted researches to overcome the fact that the research on the development of the native species is very rare, unlike the study on the new variety of foreign countries, and as a result, it has been found out that there is no visible thorn variant, The present invention has been accomplished on the basis of the fact that the quality of the plant is fixed by the silkworm breeding method according to the present invention. Thus, the present invention proposes a plan to actively cope with the international convention on plant protection such as the UPOV Convention and to secure a large amount of new varieties with excellent value.

Accordingly, the present invention provides a silent propagation method for a silkworm-free variegated chrysanthemum, characterized in that the silkworm is smooth and has no visible spots on the stem, shoots and shoots, The present invention also aims to provide a method for mass production by increasing the germination rate of the females seed.

Fig. 1 is a photograph of Cheongsong 1 among the thornless mutants selected in Cheongsong, Gyeongbuk province.

FIG. 2 is a photograph showing a process of fixing a quality by grafting and approximating a non-visible mutant of a mutant,

FIG. 3A is a photograph showing a process of inducing a variety by grafting the seedlings from the seedling and harvesting the seedlings at the first year.

FIG. 3B is a photograph showing the appearance of a plant after being induced into a shallow-root graft,

FIG. 3c is a photograph showing a new kind of a new plant of the genus Lilium,

FIG. 4A is a photograph showing a process of extracting roots from the seeds and inducing the cultivars by close-fitting. FIG.

FIG. 4B is a photograph showing a state in which roots are picked up from the seeds and mass-proliferated by close-fitting,

4cThe It is a photograph showing a new varieties of pinewood,

FIG. 5A is a photograph showing the seed germination state by sowing of a seed of a females subjected to germination promoting treatment,

FIG. 5B is a photograph showing a mass propagation state by sowing of a seed of a Japanese oak plant subjected to germination promoting treatment.

In order to accomplish the above object, the present invention provides a silent propagation method of a non-visible variant of the present invention, wherein the silk is free from visible light, stem and shoot, and the bark is smooth, The present invention also provides a method for enhancing the germination rate by controlling the plant growth regulator and seedling in the seeds of the females.

Hereinafter, the present invention will be described in detail.

The mutant used as a parameter in the present invention is two individuals (Fig. 1) unearthed in a natural population in Cheongsong, Gyeongbuk Province, and has no visible, short and smooth bark. Among the above two individuals, Cheongsong No. 1 is 31 years old, 7.8 m in height, 26 cm in diameter, 3.5 m in width, and is characterized by large leaves, smooth bark, no visible spots in the stem, and shoots.

In the present invention, the variety was fixed by grafting and close-fitting over three years from 1998 to March 2000 using the mutant as a parameter. As a result of observing the traits of the rootstocks, It was confirmed that the trait was continuously expressed.

The new varieties of non-visible barks of the present invention have a sheath thickness of 8.84 ± 0.39 mm, thinner than the normal visible barks, 16.26 ± 0.62 cm in leaf length, 14.42 ± 0.23 cm in leaf width, 1.05, and is characterized by more than ordinary visible bamboo.

In the present invention, as a silent propagation method capable of stably propagating the mutant, it is preferable to use a shallow graft using a perennial seedling or a root cutting method, but the present invention is not limited thereto. In case of grafting, it is preferable to collect the graft at the beginning of March, before the sap moves, and it is effective to use it while keeping it at low temperature of 3 ~ 4 ℃ by putting it in a plastic bag after wrapping it on moss so as not to dry. As the number of trees grows larger and the number of thorns grows larger, the number of trees in a tree is difficult to graft, and it is difficult to graft and the rate of survival is low. Therefore, it is more effective to increase survival rate by grafting with 1 year old ghost tree. The grafting is preferably carried out around March 10 to April 10.

In the case of rootstock cutting, rootstock cutting was taken from the parameters and prepared to a certain size. Then, the rootstock was scraped in the rootstock containing the plant growth regulator in order to promote the rooting of the cutting. The temperature was about 60% ℃ is more effective to increase the rate of survival.

It is preferable to use the upper end of the root roots near the upper part of the root of the roots, and the rootstock rooting rate is significantly different according to the receipt of the parameters. Therefore, it is preferable to use the root roots less than 5 years for mass propagation. It is recommended that the thickness of the sample is 8 ~ 12mm and the length is 10 ~ 14cm and it should be stored at 3 ~ 4 ℃. As the plant growth regulator, it is preferable to use indolebutyric acid (IBA) and indoleacetic acid ) Alone or in combination in a range of 500 to 1,000 ppm is preferable for promoting rooting of the cutting.

Peat-Moss potting compost, Perlite, Vermiculite, and sand may be used alone or in an appropriate amount as a mixture of peatmoss culture supernatant and perlite. It is preferable to use it in the range of 1: 1 to 2.5: 1 (V / V) in order to increase the survival rate. In the preferred embodiment of the present invention, the rate of survival using various culture supernatants was examined. As a result, the best survival rate was observed in the soil mixed with 1: 1 mixture of peatmoss culture and perlite. Especially, 98% were mobilized. Therefore, considering that more than 10 shoots per root and more than 5 shoots per root shoot are taken into consideration, it is possible to massively propagate the present non-visible shoots by the root cutting. After cutting, the humidity is preferably 55 to 65%, and the temperature is preferably maintained at 26 to 30 DEG C, more preferably at 60 DEG C and 28 DEG C.

Generally, it is known that the seeds of ginseng are double dormant seeds and have germination characteristics for two years, so that germination rate is very low, about 8% on average. Therefore, in order to increase the germination rate of the seedless seeds of the present invention, the germination characteristics of the plant growth regulators according to the kind, concentration, post-treatment treatment and duration of the plant growth regulator were examined. As a result, about 800-1,500 ppm of GA ₃ (gibberellin) And germination rate of about 10 weeks after storage was observed. Especially, when 1,000 ppm of GA ₃ was applied and after 10 weeks of storage, the highest number of germination occurred between 3 and 4 weeks of gestation and the germination rate reached 90%. The method of storing the temperature is as follows. The seeds are collected in a plastic bag immediately after harvesting and seeded at room temperature. The seeds are grown in a ratio of 2: 1 with sand at a temperature of 3-4 ° C for 1 week and 15-16 ° C for 1 week alternately for 10 weeks. It is preferable to immerse for 72 hours.

The bamboo shoots of the present invention propagated through the process described above can be widely used as a finest wood, a hair tonic, a neuralgia, etc., and can be used as a source of saponin, lignin or antioxidants, And can be used as a high-grade alive with high preference.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

≪ Example 1 > Selection of mutant mutants

The mutant used in the present invention was two individuals unearthed in a natural mulberry cultivated on a 400m elevation in the Hyeondong-myeon area, Cheongsong-gun, Gyeongbuk, Korea in March 1998. The mutant has no visible, Has a smooth characteristic. Among these variants, Cheongsong No. 1 has 31 years of age, 7.8 m in diameter, 26 cm in diameter, 3.5 m in diameter, and 31.3 years in Chongsong No. 2, 8.5 m in height, 31 cm in diameter and 4.0 m in width. Table 1 shows the results of comparing the characteristics of ordinary wood.

characteristic Mutant General object Bark character Endothelial thickness (mm) 4.64 4.42 Shell thickness (mm) 8.84 13.95 Bark weight (g) 0.33 0.30 Visibility none has exist Leaf characteristic Leaf length (cm) 16.26 14.38 Leaf width (cm) 14.42 11.43 Petiole length (cm) 17.06 16.58 Number of corners () 6 7 Dong-A Characteristics Base diameter (cm) 1.14 0.92 Number of cardiac events () 7 3

As can be seen from the above, the selected mutants are characterized by a smooth appearance of the bark, a thin outer skin, and a significantly increased number of lateral buds, as compared with the normal bamboo trees (see Fig. 1).

≪ Example 2 > Investigation of the activation efficiency according to grafting conditions

1) Survival rate by grafting method and timing

In order to induce the cultivar of the non-visible bamboo selected in Example 1, grafting was carried out over three years of 1998, 1999 and 2000 (Figs. The receipt was taken at the beginning of March, before the liquid was transferred, and it was wrapped in moss so that it did not dry. Then, it was stored in a low temperature storage at 3-4 ℃ in a plastic bag, and the survival rate was examined by the grafting method and the period from the beginning of March of the same year.

Grafting method Survival rate by grafting time (%) March 10 March 20 March 30 April 10 Grafted graft 68 76 88 72 Fold 54 58 64 56

As a result, the survival rate was the highest when the grafting was carried out around March 30, and it was confirmed that the grafting method was more effective than the arched grafting for the grafting.

2) The survival rate

Table 3 shows the survival rates according to the receipt of the larvae at the age of 1, 2, 3, and 5 years, respectively.

Large-size order receipt (year) Number of grafting (main) Number of streaks (Bon) Stable rate (%) 1 year 100 88 88 2 years 60 48 67 3 years 60 32 53 5 years 60 24 40

As can be seen from the above, the survival rate was higher with lower yields of seedlings, and the highest with 88% survival rate when using 1 - year - old seedlings.

Considering the above results, it was confirmed that the best survival rate was 88% when the first year old young timber was used for mariculture on March 30.

≪ Example 3 > Surveying activity rate of extrapolation according to the type of soil

In order to investigate the possibility of breed fixation by root cutting, we investigated the survival rate of root cutting by root (Figs. 4a to 4c). The experimental method was as follows: First, the endoprosthesis was taken from the end of March, 1998, before the transfer of the fluid. The sample was stored at 3-4 ℃ in a low temperature storage tank. Then, the sample was cut into 12 ± 2cm lengths and then vermiculite, perlite, , Sand and peat-moss potting compost (PKS2) were individually or mixed in a mixed soil. After the root cutting, humidity was 60% and the temperature of the cutting was kept within 28 ℃.

Sangto Number of cuttings (bones) Number of streaks (Bon) Stable rate (%) sand 60 16 27 PKS2 + sand + pearlite (1: 1: 1) 60 22 37 Vermiculite + pearlite (1: 1) 60 11 18 PKS2 + pearlite (1: 1) 60 29 48 PKS2 60 24 40

The rooting rate of root crops was higher than that of cultivated peatmoss cultivated soil. Especially, when the soil was mixed with 1: 1 mixture of peatmoss cultured soil and perlite, the highest survival rate was 48% The possibility of fixing was confirmed. Also, it showed a high rate of survival in samples collected from the soil surface near the ground surface.

<Example 4> Investigation of root cutting conditions for mass propagation

In order to investigate the possibility of mass proliferation by root cutting, root cuttings were cut into 12 ± 2cm length with 1 year old root as a sample, and the rooting rate was examined after root cutting per culture soil.

Sangto Upper layer Middle layer portion Lower layer sand 80 58 25 PKS2 + sand + pearlite (1: 1: 1) 84 60 28 Vermiculite + pearlite (1: 1) 78 54 26 PKS2 + pearlite 98 67 42 PKS2 92 64 40

As a result of the activation rate by site and culture soil, 98% of the top layer samples mixed with peatmoss culture and perlite (1: 1) showed the best survival rate, and 67% of the middle layer and 42% of the lower layer And there was a significant difference in the number of shoots and growth. Especially, in the case of the upper part of the perennial seedling of 1-year-old seedlings, 98% of the seedlings are allowed to grow, and more than 10 rootstocks per 1-year-old seedlings can be produced and more than 5 shoots per per- Mass proliferation is expected to be possible.

Example 5: Promotion of germination of females seeds by plant growth regulators and post-mortem treatment

Table 6 shows the germination characteristics according to the concentration of gibberellin, a plant growth regulator, and post-treatment treatment method, in order to find the optimum breeding condition by seeding. In the following Table 6, gibererin was treated at 0 to 1,500 ppm. As a post-treatment method, the seeds were stored in a dark place at room temperature, 2: 1, and then stored at about 4 ° C. The open-air store was kept in a well-drained oven where the wet sand was mixed with the seeds. On the other hand, after preserving the seeds, the seeds were subjected to gibberellin treatment for about 1 week at 3-4 ° C, about 1 week at 15-16 ° C for about 10 weeks, and then immersed in flowing water for about 72 hours .

process GA3 concentration (ppm) 0 10 100 500 1,000 1,500 Save the Governor 0 0 0 0 5 5 Housekeeping refrigeration 45 52 57 50 71 52 Saving the extreme temperature change 69 48 67 62 90 74 Open-air store 60 38 55 60 71 69

As a result, the germination rate of the seeds treated with the humectant-treated seeds was significantly higher than that of the other treatments, and the seed germination rate was 90% in the case of 1000 ppm of gibberellin, . On the other hand, the germination was the most abundant when germination was 3-4 weeks. Therefore, it was confirmed that the seed germination rate of about 8% could be increased to more than 90% by controlling the temperature storage and the gibberellin concentration. (Figs. 5A and 5B)

The method of silkworm propagation without a visible mark according to the present invention has an advantage of allowing a large amount of stably grown plants to be obtained as well as expressing the same genetic traits as the parameters of seeds. Can be increased to 90% or more.

Claims (14)

delete delete In the non-reproductive propagation method of the variegated chrysanthemum having the characteristics of smoothness of the bark without any visible spots on the leaves, stems and shoots, the receipt was collected before the transfer of the sap and stored at a low temperature of 3 캜 to 4 캜, A method of silent propagation of a bamboo without a thorn due to grafting, characterized by grafting to an annual tree. delete 4. The method according to claim 3, wherein the grafting is carried out around March 10 to April 10. In the silent propagation method of the variegated chrysanthemum which has no visible and smooth bark in a few days, stems and shoots, 1) Obtaining the root cutting from the uppermost layer nearest to the ground surface from the soil surface in the root roots; 2) cutting the above-mentioned root cutting in the root of the rootstock containing the plant growth regulator; And 3) The step of activating the slices by keeping the slices at a temperature of 26 to 30 DEG C with a humidity of 55 to 65%, and activating the slices. 7. The method according to claim 6, wherein the cutting in step 1 is made at a length of 12 2 cm from the uppermost layer nearest to the top of the soil surface in the root roots. 7. The method according to claim 6, wherein the plant growth regulator of step 2 is indolebutyric acid (IBA) or indoleacetic acid (IAA) or a mixture thereof. [7] The cutting method according to claim 6, wherein the scalloped soil of step 2 is a culture soil containing peat moss culture supernatant (PKS2), vermiculite, perlite and sand alone or a mixture thereof. Reproduction method. [10] The method according to claim 9, wherein the scalloped soil is a culture soil in which peatmoss cultured soil and perlite are mixed at a ratio of 1: 1 to 2.5: 1. The present invention relates to a method for promoting the germination of a variegated seed of a plant having no visible and smooth bark on a stem, a shoot and a shoot, characterized in that the plant growth regulator is treated with a plant growth regulator and germination is carried out by heat- , A method for promoting germination of a females seed. 12. The method according to claim 11, wherein the plant growth regulator is gibberellin and the treatment concentration is 800 to 1,500 ppm. 13. The method according to claim 11 or 12, 1) harvesting seeds; 2) immersing the seed in a plant growth regulator solution containing 1,000 ppm of gibberellin; 3) thermally storing the seeds at 3 to 4 캜 for 1 week and at 15 to 16 캜 for 1 week alternately for 10 weeks; 4) immersing the seed in running water for 72 hours. 7. The method according to claim 3 or 6, wherein said receiving or cutting is obtained from a bamboo shoot without bosoms having the following characteristics. Sheath thickness 8.84 ± 0.39 mm Leaf length 16.26 ± 0.62cm Leaf width 14.42 ± 0.23 cm Number of cardiac events 7 ± 1.05