KR100361880B1 - Compositions of containing titrated extract of the unsaponifiable fraction of Zea Mays L. and extract of Magnoliae Cortex for prevention and treatment of periodontal disease - Google Patents

Abstract

The present invention relates to a composition for preventing and treating periodontal disease, comprising corn unchecked quantitative extract and thick extract as an active ingredient.

The composition of the present invention is a corn extract that has a prophylactic and regenerative effect against alveolar bone absorption and destruction of periodontal ligaments, and a thick leaf extract having an excellent bactericidal activity against anaerobic Gram-negative bacteria Prebotella intermedium, one of the causative agents of periodontal disease By containing as an active ingredient exhibits anti-inflammatory action, showing a significantly superior effect of preventing and treating periodontal disease than existing periodontal disease prevention and treatment.

Description

Compositions of containing titrated extract of the unsaponifiable fraction of Zea Mays L. and extract of Magnoliae Cortex for prevention and treatment of periodontal disease}

The present invention relates to a periodontal disease prevention and treatment composition containing corn unchecked quantitative extract and thick extract as an active ingredient.

If you have periodontal disease, clinically, gingival hemorrhage, swelling, periodontal sac formation, and destruction of alveolar bone can lead to tooth loss. The causes of periodontal disease include local and systemic factors. As a local factor, when the plaque is mechanically accumulated in the periodontal sac, it becomes a habitat for the surrounding bacteria, which gradually become anaerobic, breathable, and gram-positive bacteria. It gradually migrates to negative bacteria, and then proliferates deep into the periodontal sac. At this time, the transition to the anaerobic gram-negative bacteria is gradually expanded, and proliferate deep into the periodontal sac. At this time, the toxins and all products of the proliferated anaerobic Gram-negative bacteria directly destroy tissues or stimulate the immune system, thereby causing periodontal tissue destruction and inflammation by various actions from the stimulated immune system.

As a defense mechanism, the function and immune response of polymorphonuclear leukocytes act as systemic factors. However, antimicrobial and bacteriostatic effects on anaerobic gram-negative bacteria, which are the underlying factors, and removal of toxic products of these bacteria and restoration of destroyed and lost periodontal tissues are known to be important for prevention and treatment of periodontal disease. have.

The prevention and treatment of periodontal disease has been conducted a lot of research since the 1920s, especially in Western countries have focused on the removal of plaque using antibiotics and chemotherapy. These studies have been developed more actively since 1970 when the contours of periodontal bacteria among the bacteria in plaque have been identified, such as Listerine, a phenolic compound, Scofu, a quaternary ammonium compound, and Ferdex, a chlorhexidine product. It is currently marketed in Gumi and imported and sold in Korea.

Listerin or scopu is a chemotherapeutic agent with mild side effects, but it does not have a great effect on elimination of plaque and antimicrobial effects. Ferricex, a chlorhexidine preparation, exhibits effective removal of plaque and antibacterial effects, but has a severe side effect of 0.12%. In oral mucosal ulceration, exfoliative gingivitis, pigmentation, etc. appear, and long-term use has been reported to have side effects such as dry mouth. Therefore, in order to express a complete periodontal disease prevention and treatment effect, it is necessary to have an antimicrobial and bacteriostatic action against anaerobic Gram-negative bacteria and to remove the inflammation and to restore destroyed and lost periodontal tissue. It was.

From this point of view, the present inventors showed antimicrobial activity selectively against periodontal pathogens while researching herbal medicines useful for preventing and treating periodontal disease, while existing antibiotics or antimicrobial chlorhexidine, phenolic compound, listerin, and quaternary ammonium compound, scofu It is a product that does not have the effect of balance, long-term expression, exfoliation of periodontal tissue and risk of carcinogenicity. It has antibacterial activity against Streptococcus mutans. Published in Japanese Patent Application Publication No. 2-17524, and reported by the present inventors to have an excellent bactericidal activity against the anaerobic Gram-negative bacterium Prevotella intermedia, one of the causative agents of periodontal disease (Korea Patent Application No. 92-16516) Quantitative weight of corn unchecked commercialized commercially available pepper extract To confirm that the result of the experiment is mixed with water sikindaneun much more enhance the effects of conventional corn bulgeom quantify extract thereby completing the present invention. Corn unchecked quantitative extracts have been reported to have preventive and regenerating effects on alveolar bone absorption and periodontal ligament destruction when taken regularly ( Chaput , L'information Dentaire, 1964, 23, 2148-2153), and tooth agitation is slightly reduced. It has been reported that the periodontal pocket depth has also decreased ( Ackerman et al. L'information Dentaire, 1968, 8: 751-758). We also reported that tooth agitation was reduced and alveolar ringworm prevalence was high after administration of corn unchecked quantitative extract and modified Widman valve surgery ( Choi et al ., 1989, 19 (1): 63-. 70; Kwon et al. , Korean Journal of Periodontology, 1994, 24 (3): 649-660). It is commercially commercialized on the basis of these results and is still commercially available in various ways.

The prior art of the periodontal disease prevention and treatment composition containing the extract as an active ingredient is known in many documents or patents, for example, Republic of Korea Patent Publication No. 98-0176015, 98-0143191, Republic of Korea Patent Publication 96-0007923, The Korean Journal of Periodontal Science vol.22, No.3, 1992, vol.25, No.3, 1995, vol.26, No.2, 1996, vol.27, No.1, 1997, vol. 28, No. 4, 1998 and the like are described in the composition.

The prior arts are much safer than conventional antibiotics such as chlorohexidine, which is the causative agent of periodontal disease, and have a synergistic effect when magnols and honokiols extracted from thick husks are mixed with chlorohexidine. It has been found to exert an excellent bactericidal action on prevotella intermedia (Korean Patent Publication No. 96-0007923). Anaerobic Gram-negative bacterium Prevotella intermedia, one of the causative agents of periodontal disease, is composed of thick leaf extract with excellent bactericidal activity and ginkgo leaf extract with excellent collagenase inhibitory and tissue regeneration effect as an active ingredient. It has been shown to exhibit excellent antibacterial, bacteriostatic, anti-inflammatory and tissue regeneration effects (Korean Patent Publication No. 98-0143191), and extracts of jujube and jujube extracts containing magnool and hokinol as main ingredients are active ingredients. It has been found that it has excellent tissue regeneration, antibacterial and anti-inflammatory effects, and particularly, exhibits excellent tissue regeneration effect by overcoming the disadvantages of the extract of gourd extract which inhibits the activity of periodontal tissue cells at high temperature (Korean Patent Publication No. 98-98). 0176015).

The above-mentioned prior arts have a wide range of effects in the prevention and treatment of periodontal disease, ie, by combining the vulcan extract alone or in combination with a jujube extract, a ginkgo biloba extract, and (1) an excellent regeneration effect on weakened or destroyed periodontal tissues (2 ) It inhibits inflammatory reactions that destroy periodontal tissues, and (3) has excellent antimicrobial effects on the causative bacteria of periodontal disease, and eliminates side effects that are problems when using antibiotics. However, thick extracts have excellent anti-inflammatory and antimicrobial effects, but the higher doses have the disadvantage of lowering the activity of periodontal tissue cells. Jujube extract and ginkgo biloba extracts have excellent tissue regeneration effect but have insufficient antibacterial effect, ie prevention of periodontal disease and Among the various mechanisms for treatment, the disadvantage is that it only works through some pathways.

The present invention has been made on the basis of the above facts, the present inventors confirmed that the corn unchecked quantitative extract mixed with the thick extract and the titration ratio as a result of the experiment to further enhance the effect of the conventional corn unchecked quantitative extract. The present invention was completed. Therefore, it is an object of the present invention to provide a periodontal disease prevention and treatment composition comprising the addition of the pak extract to corn unchecked quantitative extract.

Corn unchecked quantitative extract used in the present invention was prepared by extracting the extract containing about 40% of uncompensated corn oil through continuous primary screening using a caustic soda solution and an organic solvent from the corn oil collected from the embryo of corn. Reflux with caustic soda solution, extract and concentrate the saponification, add alcohol and filter to recover the sterols and concentrate the filtrate to produce a non-compound x. Thick extract is extracted by adding alcohol to Chinese or Japanese hubak, heated, filtered and concentrated filtrate. At this time, magnolol which is a main component was used as the thick X-1.0 more than 1.0%.

Corn unchecked quantitative extract and thick extract extract composition of the present invention may be added to the usual pharmaceutical excipients, it may be formulated in the form of tablets, ointments, toothpaste liquid (gagle), spray. For example, it can be included alone in a dentifrice composition, and can be mixed with a conventional carrier and formulated as an ointment or solution to be used as a prophylactic and therapeutic agent for periodontal disease.

In addition, it may be included in a conventional gargle to rinse the mouth after brushing the teeth, or a dispersant may be packaged in a compressed container and sprayed onto the periodontal disease site, or included in the toothpaste component to continuously prevent and treat periodontal disease for a long time. You can also get In this formulation, the ratio of the corn unchecked quantitative extract and the thick extract may vary, and there is a slight difference depending on the difference in drug efficacy between the external preparation and the internal preparation. The ratio is 0.5: 1 to 1: 1 (weight ratio). The effect was the most excellent when mixed in the ratio of this range, the effect was slightly weaker when mixed at 1.5: 1 to 2.0: 1 than when mixed at 0.5: 1 to 1.0: 1.

Hereinafter, the present invention will be described in more detail through reference examples, experimental examples, and examples.

Reference Example 1: Preparation of Corn Unchecked Quantitative Extract

1 kg of corn oil from corn embryos was added to a round flask, and 10 L of n-hexane was added to make a less viscous solution. Then, 0.68 L of 5% caustic soda solution was added and refluxed at 55 ° C. for 3 hours. After cooling and left for 2 hours, it is separated into two layers. Fat, oil and free fatty acids in corn oil are mostly dissolved in the water layer by saponification and neutralization. The remaining residue was 0.045 kg in dark brown viscous material with about 40% non-gum content. This was transferred to a round flask for the second safflower, and the saponification operation was repeated twice. The non-compound obtained by this process was concentrated to obtain a dried product mainly composed of beta-sitosterol, a colorless white crystal. Gas chromatographic analysis shows that the beta-sitosterol is about 70%, the campesterol is about 6%, and the alpha-sitosterol is about 16%, and the total sterol component is about 93%.

Reference Example 2: Preparation of Thick Extract

500 g of Magnolia officinalis L. or M. obovata Thunb was cut into small pieces, and put into a round flask, and 3L of 75% ethanol was extracted for 2 hours in a 60 ° C. water bath, filtered, and filtered. The above operation was repeated on the remaining residue to obtain a secondary filtrate, and the primary and secondary filtrates were combined and concentrated under reduced pressure to obtain an ethanol extract (90 g, 18%). The extract was quantified as an indicator of magnolol, the main component by HPLC, and the one containing 0.5% or more of this substance (prescribed to 0.8% or more magnolol in Japanese pharmacy) was used in the experiment.

Reference Example 3 Preparation of a Mixture of Corn Unchecked Quantitative Extract and Thick Extract

The corn unchecked quantitative extract and the thick extract were made into 0.4% and 0.3% aqueous solution, respectively, followed by the ratio of the corn unchecked quantitative extract: thick extract = 0.5: 1, 1.0: 1, 1.5: 1, 2.0: 1 (weight ratio). The mixture was used for the experiment.

Experimental Example 1: Effect of gingival fibroblast activity of corn unchecked quantitative extract and thick extract extract

The gingival area of the first premolar was collected from patients undergoing orthodontic treatment for gingival fibroblast culture. Immediately before the collection, the calculus and plaque were removed using a curette and washed several times with physiological saline. Internal gingival resection was performed on the interdental area and normal gingival tissue was collected. The collected tissues were cultured using α-minimal essential medium (α-MEM) containing 100U / ml Penicillin, 100µg / ml Streptomycin and 10% fetal bovine serum (FBS). Five passages were incubated while replacing. 95% air and 5% CO ₂ were continuously maintained while maintaining the humidity at 95% and the temperature at 37 ° C. Subcultured gingival fibroblasts were treated with 0.25% Trypsin-EDTA solution and centrifuged to make cell suspension from the culture medium and inoculated with a standard hemocytometer with 1 × 10 ⁵ cells per well. 20 well of each extract or its mixed composition was added to each well, and 180 씩 of the culture solution was added to make 200 μl altogether. They were incubated for 24 hours while maintaining 95% humidity and 37 ° C while supplying 95% air and 5% CO ₂ , and after completion of the culture, MTT (methyl thiazol-2-YL-2,5-diphenyl tetraolium dissolved in physiological saline). bromide, Sigma Co., St. Louis, MO, USA) 50 μl of solution was added to each well and incubated for 4 hours, MTT solution was removed, and 50 μl of DMSO was added to dissolve formazon crystals. After shaking the plate well, absorbance was measured at 570 nm with an Enzyme linked immunosorbent assay (ELISA) reader (Thermo max, molecular devices, Menlo Park CAUSA). As a control, α-MEM medium well containing no experimental solution was used for each experiment. All experimental results were calculated as a percentage of the control group. Experimental materials were 0.4%, 0.3% corn unchecked quantitative extract, 0.4%, 0.3% thick gourd extract, and corn unchecked quantitative extract: thick gourd extract (0.5: 1, 1: 1, 1.5: 1, 2: 1) was used.

As a result, when the corn unchecked quantitative extract: wreak extract is 0.5: 1, the corn unchecked quantitative extract showed a significantly higher cell activity than when the corn unchecked quantitative extract alone or the hooted extract alone was present. Even when mixed 1: 1, each extract showed significantly higher cell activity than when present alone, and even though the ratio of 1.5: 1 and 2: 1 was lower than that of 0.5: 1 and 1: 1, Each extract showed significantly higher cell activity compared to when present alone. This result seems to be due to the synergism of the two extracts when the corn unchecked quantitative extract and the thick extract were combined (see Table 1.).

Effects of Each Extract and Its Mixtures on the Activity of Gingival Fibroblasts Test subject extract Cell activity (%) Cell activity increase rate (each experiment group-control group) Of cell activity % Growth (Mixture / 0.4% corn Unchecked quantitative extract) Control (Nothing added) 100 · 0.4% Corn Unchecked Extract 120.5 20.5 · 0.4% Thick Extract 120.3 20.3 · 0.4% Corn Unchecked Quantitative Extract (A) and 0.4% Thick Extract (B) A: B = 0.5: 1 142.2 42.2 205.9% A: B = 1.0: 1 138.8 38.8 189.3% A: B = 1.5: 1 138.5 38.5 188.8% A: B = 2.0: 1 122.4 22.4 109.3%

* The amount of each extract or mixture thereof used in the experiment was the same (20 μl).

* Based on 0.4% corn unchecked quantitative extract in comparison of cell activity increase rate (%).

Effects of Each Extract and Its Mixtures on the Activity of Gingival Fibroblasts Test subject extract Cell activity (%) Increase in cell activity (Each experiment group-control group) Of cell activity % Growth (Mixture / 0.3% corn Unchecked quantitative extract) Control (Nothing added) 100 · 0.3% Corn Unchecked Extract 116.7 16.7 · 0.3% Thick Extract 117.1 17.1 · 0.3% corn unchecked quantitative extract (A) and 0.3% thick extract (B) A: B = 0.5: 1 141.1 41.1 246.1% A: B = 1.0: 1 133.6 33.6 201.2% A: B = 1.5: 1 129.5 29.5 176.6% A: B = 2.0: 1 118.6 18.6 111.4%

* The amount of each extract or mixture thereof used in the experiment was the same (20 μl).

* Based on 0.3% corn unchecked quantitative extract in comparison of cell activity increase rate (%).

Experimental Example 2: Anti-inflammatory effect experiment of corn unchecked quantitative extract and thick leaf extract mixture

1) Inhibitory Effect of Fibroblasts on Interleukin-1β (IL-1β) Production

Cell counts were counted with a standard hemocytometer and then inoculated with 5 × 10 ⁵ cells per well in a 24-well plate. After incubation for 24 hours, the culture medium was removed and washed with Hank's balanced salt solutions (HBSS). After 24 hours of incubation with each extract-containing medium, the cultures were collected, washed with HBSS and collected again, and then separated with 0.02% EDTA-2.5% trypsin-PBS (Phosphate buffer solutions) (10: 1: 9). The supernatant was collected by centrifugation and the cells were collected separately. To extract IL-1β contained in the cells, the cells were freeze-thawed three times, dissolved in 0.5 ml of phosphate buffer (pH = 7.2, 13 mM phosphate, 0.15M NaCl) for 30 minutes at 4 ° C, and suspend. Centrifuge for 10 min at xg. The supernatant designated Sample A was used for the quantitative analysis of Interleukin-1β (IL-1β), and the precipitate was sonicated with branson sonifier cell disrupter B15 (Fisher, USA) for 2 minutes at 4 ° C and suspended in 0.5 ml of PBS. I was. The tube was centrifuged and the supernatant collected for IL-1β determination (Sample B). The pellet was resuspended in 0.5 ml PBS through a freeze-thaw cycle and centrifuged. Supernatants were again collected for determination of IL-1β (Sample C). The total amount of samples A, B and C containing IL-1β was added to give the total amount of IL-1β. IL-1β concentration was measured using the high sensitivity Interleukin-1β ELISA kit (Cistron Biotechnology, USA). Each extract and its mixture and non-specific binding (NSB) wells were replicated in the wells of the ELISA kit. 100 μl was added to each well, and NSB wells were used as a zero standard (Matrix with No IL-1β). The wells were covered and the plate was incubated at 37 ° C. for 20 minutes. The wells were washed three times with wash buffer. After dispersing 200-300 μl of each well as buffer during the washing process, aspiration after 30 seconds was repeated to remove the remaining wash buffer with a clean paper towel after the last wash. 100 μl IL-1β antiserum (rabbit) was added to each well and incubated at 37 ° C. for 20 minutes. After repeated washing in the above manner, 100 μl of anti-rabbit IgG-HRP conjugate was added to each well. After the wells were covered, the plates were incubated at room temperature for 20 minutes, washed with the method described above, and incubated at room temperature for 10 minutes with 100 μl IL-1β antiserum (rabbit) added to each well. 50 μl of 4N sulfuric acid was added to each well and measured within 15 minutes. The microtitration plate reader (THERMO maxTM, Molecular Devices Co., USA) was fitted at 450 nm and absorbance was measured.

In experiments on the production blocking effect of interleukin-1β, inhibition of corn unchecked quantitative extract: thick extract 0.5: 1 compared to 0.4% and 0.3% corn unchecked quantitative extract alone. The effect was much greater. In this case, synergism was shown by mixing the two extracts. This synergism was also seen in the composition of 1: 1 mixed corn unchecked quantitative extract: thickness extract, and interleukin was also tested in corn unchecked quantitative extract alone in 1.5: 1 and 2: 1. The effect of inhibiting the production of -1β was excellent (see Table 2).

Inhibitory Effect of Each Extract and Its Mixture on Interleukin-1β (IL-1β) Production Test subject extract Production of Interleukin-1β (pg) Comparison of Production Inhibition Rate of Interleukin-1β (100-Mix / 0.4% Corn Unchecked Quantitative Extract (%)) Control 89 0.4% Corn Unchecked Extract 40 · 0.4% Thick Extract 26 · 0.4% Corn Unchecked Quantitative Extract (A) and 0.4% Thick Extract (B) A: B = 0.5: 1 20 50% A: B = 1.0: 1 24 40% A: B = 1.5: 1 25 37.5% A: B = 2.0: 1 28 30%

* The control group was stimulated with Lipopolysaccharide, and there was no special administration.

* The experimental group was dosed with each test drug after stimulation with Lipopolysaccharide.

Lipopolysaccharide: A substance that stimulates interleukin-1β production.

Interleukin-1β: mediator of inflammation.

* The amount of each extract or mixture thereof used in the experiment was the same (20 μl).

* pg: picogram

* Based on 0.4% corn unchecked extract from Interleukin-1β production inhibition rate (%).

Inhibitory Effect of Each Extract and Its Mixture on Interleukin-1β (IL-1β) Production Test subject extract Production of Interleukin-1β (pg) Comparison of Production Inhibition Rate of Interleukin-1β (100-Mix / 0.3% Corn Unchecked Quantitative Extract (%)) Control Group (Lipopolysaccharide Stimulation Group) 89 · 0.3% Corn Unchecked Extract 44 · 0.3% Thick Extract 28 · 0.3% corn unchecked quantitative extract (A) and 0.3% thick extract (B) A: B = 0.5: 1 24 45.5% A: B = 1.0: 1 26 40.9% A: B = 1.5: 1 29 34.1% A: B = 2.0: 1 31 29.5%

* The control group was stimulated with Lipopolysaccharide, and there was no special administration.

* The experimental group was dosed with each test drug after stimulation with Lipopolysaccharide.

Lipopolysaccharide: A substance that stimulates interleukin-1β production.

Interleukin-1β: mediator of inflammation.

* The amount of each extract or mixture thereof used in the experiment was the same (20 μl).

* pg: picogram

* Based on 0.3% corn unchecked quantitative extract in comparison of% of production inhibition of interleukin-1β.

2) Prostaglandin E in Fibroblasts ₂ (PGE ₂ A) production blocking effect

Five to seven passages of gingival fibroblasts were dispensed into 10 ⁵ cells / well in α-MEM medium in a 24 well plate and then recombinant Human Interleukin-1β (rHuI-1-1, Genzyme, Co., Cambridge, MA, USA) ) 1ng / ml was added to induce the production of Prostaglandin E ₂ (PGE ₂ ), and the effect of blocking PGE ₂ production was observed by adding each extract and its mixture. The well to which no additive was added was used as a control. After aseptically culturing each well for 48 hours, the medium of each well was collected, and the PGE ₂ in the medium was colorimetrically weighed at 450 nm with an ELISA reader using a PGE ₂ enzyme immunoassay system (Amersham, In, Buckinghamshire, UK). .

In the experiments on the effect of PGE ₂ on production, the effect of inhibiting the composition of the corn unchecked quantitative extract: Thick extract 0.5: 1 compared to 0.4% and 0.3% corn unchecked quantitative extract alone. Was much bigger. In this case also, synergism was shown by mixing the two extracts. The inhibitory effect was also observed in the compositions mixed 1: 1 and 1.5: 1 and also in the compositions mixed 2: 1 (see Table 3).

In these two experiments, it was confirmed that the mixed composition of the corn unchecked quantitative extract and the thick extract showed anti-inflammatory action, and the effect was superior to that of the corn unchecked quantified extract alone. This anti-inflammatory effect was better when the ratio of corn unchecked quantitative extract: thick extract was 0.5: 1, 1.0: 1.

Blocking Effect of Prostaglandin E ₂ (PGE ₂ ) Production from Each Extract and Its Mixtures Test subject extract Prostaglandin E ₂ Yield (pg) Prostaglandin E ₂ Production inhibition rate (100-Mix / 0.4% Corn Unchecked Quantitative Extract (%)) Control 62 0.4% Corn Unchecked Extract 16.5 · 0.4% Thick Extract 8.6 · 0.4% Corn Unchecked Quantitative Extract (A) and 0.4% Thick Extract (B) A: B = 0.5: 1 6.8 58.8% A: B = 1.0: 1 7.8 52.7% A: B = 1.5: 1 7.6 53.9% A: B = 2.0: 1 8.6 47.9%

* The control group did not have any special medication after stimulation with Interleukin-1β.

* The experimental group received each drug after stimulation with Interleukin-1β.

* Interleukin-1β: A substance that stimulates Prostaglandin E ₂ production.

Prostaglandin E _{2 is a} mediator of inflammation.

* The amount of each extract or mixture thereof used in the experiment was the same (20 μl).

* pg: picogram

* Based on 0.4% corn unchecked quantitative extract in comparison of% inhibition of production of Prostaglandin E ₂ .

Blocking Effect of Prostaglandin E ₂ (PGE ₂ ) Production from Each Extract and Its Mixtures Test subject extract Prostaglandin E ₂ Yield (pg) Prostaglandin E ₂ Production inhibition rate (100-Mix / 0.3% Corn Unchecked Quantitative Extract (%)) Control 62 0.3% Corn Unchecked Extract 18 · 0.3% Thick Extract 10.5 · 0.3% corn unchecked quantitative extract (A) and 0.3% thick extract (B) mixture A: B = 0.5: 1 7.9 56.2% A: B = 1.0: 1 8.6 52.2% A: B = 1.5: 1 9.6 46.7% A: B = 2.0: 1 10.5 41.7%

* The control group did not have any special medication after stimulation with Interleukin-1β.

* The experimental group received each drug after stimulation with Interleukin-1β.

* Interleukin-1β: A substance that stimulates Prostaglandin E ₂ production.

Prostaglandin E _{2 is a} mediator of inflammation.

* The amount of each extract or mixture thereof used in the experiment was the same (20 μl).

* pg: picogram

* Based on 0.3% corn unchecked quantitative extract in comparison of% inhibition of production of Prostaglandin E ₂ .

The combination composition of the corn unchecked quantitative extract of the present invention and the thick extract extract may be prepared by conventional pharmaceutical methods, for example, in the form of tablets, ointments, toothpastes, solutions, sprays, and the ratio of the two extracts is preferably The ratio of the corn unchecked quantitative extract: thick extract is 1: 0.5 to 1: 2 (weight ratio), particularly preferably 1: 0.5 to 1: 1 (weight ratio), and examples of the compositions are as follows.

Hereinafter, the specific configuration and operation of the present invention will be described in more detail by way of examples, but the present invention is not limited by the following examples.

Example 1: Film coated tablets-1 tablet (in 600 mg)

Corn unchecked quantitative extract 20mg

Thick Extract 40mg

Lactose 525mg

5 mg of hydroxypropyl cellulose

Magnesium Stearate 3mg

Glycerin 2mg

Hydroxypropylmethylcellulose 5mg

Prepared according to the conventional tablet production method, and take one tablet three times a day, once as a dose.

Example 2: Film coated tablets-1 tablet (in 600 mg)

Corn unchecked quantitative extract 30mg

Thick Extract 30mg

Lactose 525mg

5 mg of hydroxypropyl cellulose

Magnesium Stearate 3mg

Glycerin 2mg

Hydroxypropylmethylcellulose 5mg

Prepared according to the conventional tablet production method, and take one tablet three times a day, once as a dose.

Example 3: film coated tablets-1 tablet (in 600 mg)

Corn unchecked quantitative extract 40 mg

Thick Extract 20mg

Lactose 525mg

5 mg of hydroxypropyl cellulose

Magnesium Stearate 3mg

Glycerin 2mg

Hydroxypropylmethylcellulose 5mg

Prepared according to the conventional tablet production method, and take one tablet three times a day, once as a dose.

Example 4 Ointment (Value by Weight)

Corn Unchecked Quantitative Extract 0.20

Thick Extract 0.40

Carboxy Vinyl Polymer 7.00

Glycerol 30.00

Peppermint Oil 0.006

Propylene Glycol 30.00

Distilled water is added to make 100.

The composition is mixed by a conventional ointment preparation method to prepare an ointment, and is often applied around the teeth with periodontitis in the oral cavity to treat periodontitis or gingivitis.

Example 5 Ointment (Value by Weight)

Corn Unchecked Quantitative Extract 0.30

Thick Extract 0.30

Carboxy Vinyl Polymer 7.00

Glycerol 30.00

Peppermint Oil 0.006

Propylene Glycol 30.00

Distilled water is added to make 100.

The composition is mixed by a conventional ointment manufacturing method to prepare an ointment and is often applied around the teeth with periodontitis in the oral cavity to treat periodontitis or gingivitis.

Example 6 Ointment (Value by Weight)

Corn Unchecked Quantitative Extract 0.40

Thick Extract 0.20

Carboxy Vinyl Polymer 7.00

Glycerol 30.00

Peppermint Oil 0.006

Propylene Glycol 30.00

Distilled water is added to make 100.

The composition is mixed by a conventional ointment manufacturing method to prepare an ointment and is often applied around the teeth with periodontitis in the oral cavity to treat periodontitis or gingivitis.

Example 7 Ointment (Value by Weight)

Corn Unchecked Quantitative Extract 0.40

Thick Extract 0.80

Carboxy Vinyl Polymer 7.00

Glycerol 30.00

Peppermint Oil 0.006

Propylene Glycol 30.00

Distilled water is added to make 100.

The composition is mixed by a conventional ointment preparation method to prepare an ointment and is often applied around the tooth with oral periodontitis to treat periodontitis or gingivitis.

Example 8 Ointment (Value by Weight)

Corn Unchecked Quantitative Extract 0.60

Thick Extract 0.60

Carboxy Vinyl Polymer 7.00

Glycerol 30.00

Peppermint Oil 0.006

Propylene Glycol 30.00

Distilled water is added to make 100.

The composition is mixed by a conventional ointment manufacturing method to prepare an ointment and is often applied around the teeth with periodontitis in the oral cavity to treat periodontitis or gingivitis.

Example 9 Ointment (Value by Weight)

Corn Unchecked Quantitative Extract 0.80

Thick Extract 0.40

Carboxy Vinyl Polymer 7.00

Glycerol 30.00

Peppermint Oil 0.006

Propylene Glycol 30.00

Distilled water is added to make 100.

The composition is mixed by a conventional ointment manufacturing method to prepare an ointment and is often applied around the teeth with periodontitis in the oral cavity to treat periodontitis or gingivitis.

Example 10: Spray (Values in Weight)

Corn Unchecked Quantitative Extract 0.20

Thick Extract 0.40

Trichloromonofluoromethane 40.00

Dichlorodifluoromethane 44.85

Peppermint Oil 0.15

Ethanol 6.00

Polyethylene Glycol 8.40

The composition can be prevented and treated by mixing the composition in a conventional method for preparing a spray and spray-packing the compression container in a compressed container at any time.

Example 11: Spray (Values by Weight)

Corn Unchecked Quantitative Extract 0.30

Thick Extract 0.30

Trichloromonofluoromethane 40.00

Dichlorodifluoromethane 44.85

Peppermint Oil 0.15

Ethanol 6.00

Polyethylene Glycol 8.40

The composition can be prevented and treated by mixing the composition in a conventional method for preparing a spray and spray-packing the compression container in a compressed container at any time.

Example 12: Spray (Numeric Values)

Corn Unchecked Quantitative Extract 0.40

Thick Extract 0.20

Trichloromonofluoromethane 40.00

Dichlorodifluoromethane 44.85

Peppermint Oil 0.15

Ethanol 6.00

Polyethylene Glycol 8.40

The composition can be prevented and treated by mixing the composition in a conventional method for preparing a spray and spray-packing the compression container in a compressed container at any time.

As described above and can be seen through the examples, the composition of the present invention is anaerobic Gram-negative bacteria prevo, which is one of the causative extracts of corn unchecked quantitative extracts and the causative agent of periodontal disease, which has a prophylactic and regenerative effect on alveolar bone absorption and periodontal ligament destruction. It contains synergistic anti-inflammatory action by containing thick extract extract having excellent bactericidal action against tella intermediate as an active ingredient, thereby effectively preventing and treating periodontal disease.

Claims (3)

A periodontal disease prevention and therapeutic agent composition comprising corn unchecked quantitative extract and thick gourd extract as an active ingredient, and a blending ratio of corn unchecked quantitative extract and thick gourd extract is 0.5: 1 to 2.0: 1 by weight. The method of claim 1, wherein the ratio of the corn unchecked quantitative extract and the thick extract extract is 0.5: 1 to 1.0: 1 by weight ratio, the composition for preventing and treating periodontal disease. The composition for preventing and treating periodontal disease according to claim 1 or 2, wherein the formulation of the composition is selected from tablets, ointments, or sprays.