KR100355106B1 - Nurient-spray based plant tissue culture system capable of continuous measurement of fresh weight of in vitro plant - Google Patents

Abstract

The present invention relates to a medium injection plant culture system capable of continuous weighing of cultured plants, and improves the growth environment of the cultured plants during the nutrient breeding and to continuously measure the weight of the cultured plants in the reactor aseptically ( 3) is installed on the load cell (4) as a weighing device, and the culture support (5) made of a stainless steel net inside the bioreactor (3) to cultivate the plant on the top, the culture support (5) The medium is sprayed upward from the bottom by using the medium injection nozzle 6 installed at the bottom to supply nutrients to the plant, and the injected medium is recovered to the medium storage tank 7 outside the bioreactor 3, Plant culture for measuring the weight change of the culture plants by measuring the weight of the bioreactor (3) installed on the load cell (4) after a certain time after the medium inside the bioreactor (3) is discharged By providing the system, it is possible to continuously measure the weight of cultured plants while maintaining the proper growth environment of the cultured plants through the medium injection method during the nutrient propagation. There is an excellent effect to control the growth environment.

Description

Medium-spray based plant tissue culture system capable of continuous measurement of fresh weight of in vitro plant}

The present invention relates to a medium injection plant culture system capable of continuous weighing of cultured plants, and more particularly, to the growth environment of cultured plants in nutrient breeding by introducing a medium spraying method and a continuous weighing method of cultured plants in a bioreactor. The present invention relates to a medium-jet plant culture system capable of continuously weighing a culture plant capable of continuously measuring the weight of the culture plant in a reactor aseptically.

In general, the cultivation method applied to the plant culture is divided into a method using a solid medium and a method using a liquid medium, a liquid solution culture method for culturing plant cells and organs is mainly used for liquid culture.

In the case of the liquefied liquid culture method, oxygen is supplied by injecting air into the bioreactor filled with a liquid medium. Accordingly, the production efficiency of the culture plants is high during the nutrient breeding, but the culture plants are always submerged in the medium. However, it is impossible to continuously weigh the cultures because the growth of organs cannot be maintained optimally and the risk of contamination is increased by frequently opening and closing the inside of the culture system that must maintain aseptic status.

The level control liquid culture method of the Ebb flow method improves the growth conditions by increasing the time that the culture plants are exposed to the air by periodically changing the level of the liquid medium in the bioreactor. The same problem occurs as the dipping solution, and it is impossible to measure the weight of the culture continuously.

In a culture method using a fog generator, an ultrasonic vibrator is used to prepare the liquid medium into fine particles, and the generated fine particles of the liquid medium are contacted with the cultured plant while being sprayed into the air in the reactor to supply nutrients. In this case, it is possible to continuously weigh the cultures, but the generation of fog in the bioreactor may cause vitrification of the culture seedlings due to the high relative humidity and the light blocking effect.

Although it is necessary to measure the growth amount (weight) in order to analyze the growth state of the culture plants in the bioreactor, it is practically impossible to measure the weight of the culture plants continuously while maintaining a good growth environment in most liquid culture methods as described above. .

Therefore, an object of the present invention is to solve the above problems, to maintain the proper growth environment of the culture plants in the breeding season and continuously aseptic weight of the culture plants without causing contamination in the bioreactor To provide a plant culture system that can be measured.

Another object of the present invention is to provide a medium feeding method and a continuous weighing method of the culture plants in the plant culture system.

Hereinafter, the specific configuration and operation of the present invention.

1 is a perspective view schematically showing a medium-jet plant culture system according to a preferred embodiment of the present invention,

Figure 2 is a perspective view showing in detail the medium-jet plant culture system according to the present invention,

Figure 3 is a perspective view showing a continuous weighing unit of the medium injection plant culture system according to the present invention,

Figure 4 is a perspective view showing a medium injection device portion of the medium injection plant culture system according to the present invention.

<Description of the symbols for the main parts of the drawings>

1: culture chamber 2: control unit

3: bioreactor 4: load cell (weight measuring device)

5: culture support 6: medium dispensing nozzle

7: Medium storage tank 8: Medium circulation pump (interlocking pump)

9 pH measuring device (pH meter) 10 Air filter

11: air circulation pump (vacuum pump) 12: tube

13 artificial light source 14 computer (PC)

15 bioreactor support 16 measurement / control cable

17: discharge outlet 18: air inlet

19 air outlet

In order to achieve the above object, the present invention is to supply the medium to the culture plants by injecting the medium upward from the medium injection nozzle installed on the lower side of the culture support that is installed inside the bioreactor, installed on the bottom of the bioreactor The present invention provides a plant culture system capable of aseptically measuring the weight change of a cultured plant by measuring the weight of the bioreactor changed when the medium supply is stopped using a load cell.

The plant culture system according to the present invention is a bioreactor (3) for culturing a target plant, a load cell (weighing device) (4), which can continuously measure the weight of the bioreactor (3), inside the bioreactor (3) Medium is installed upward from the lower part of the culture support (5) by using the culture support (5), the medium circulation pump (interlocking pump) 8 and the medium injection nozzle (6) in the form of a stainless steel net to support the culture plants A medium spray device unit for spraying the medium, a medium storage tank for storing the culture medium for the culture (7), a pH measuring device (9) installed inside the medium storage tank (7), to introduce or discharge the outside air into the bioreactor (3) With air circulation pump (vacuum pump) 11, artificial light source 13 for plant culture, which is a source of energy for cultivating plants, and temperature control device, main culture chamber 1 for plant cultivation, and computer 14, in which the devices are installed. It is provided with a control unit 2 for controlling the overall plant culture system. The.

In addition, the medium spraying method and the continuous weighing method of the culture plants are sprayed on the medium upward from the medium spraying nozzle (6) installed in the lower portion of the culture support (5) using a stainless steel network installed in the bioreactor (3) Supplying a medium to the culture plants on the culture support (5); After a certain period of time, the supplied medium is discharged from the bioreactor 3 to the medium storage tank 7, and then the weight change of the cultured plant is measured by measuring the weight of the changed bioreactor 3 using the load cell 4. It includes a step.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings, but the scope of the present invention is not limited only to the following examples.

1 is a perspective view schematically showing a medium-injected plant culture system according to a preferred embodiment of the present invention, the plant culture system according to the present invention is apparently the main culture comprising a bioreactor (3) and a load cell (4) It consists of the control unit 2 connected to the chamber 1 and the computer 14.

Figure 2 is a perspective view showing in detail the medium injection plant culture system according to the present invention, the plant culture system according to the present invention comprises a main culture chamber (1) and the control unit (2). The culture chamber 1 is a load cell for weighing the bioreactor 3 installed at the bottom of the bioreactor 3 for plant culture, the bioreactor support 15 supporting the bioreactor 3, and the bioreactor support 15. (4), the culture support (5) installed inside the bioreactor (3) to support the culture plants, the medium injection nozzle (6) installed at the bottom of the culture support (5), the medium storage tank for storing the culture medium ( 7), the medium circulation pump (8) for supplying or recovering the medium in the medium storage tank (7) to the bioreactor (3), the pH measuring device (9) installed inside the medium storage tank (7) for measuring the pH of the medium, Air filter 10 to prevent contamination of cultured plant, air circulation pump 11 for introducing or discharging external air into bioreactor 3, tube 12 which is a medium and air flow passage 12, energy of cultured plant It is composed of an artificial light source 13, a medium discharge port 17, an air inlet 18, and an air outlet 19 as a supply source. The control unit 2 is connected to the computer 14 by the measurement / control cable 16, and controls the overall plant culture system.

Figure 3 is a perspective view showing a continuous weighing unit of the medium injection plant culture system according to the present invention, the load cell (4) as a weighing device is installed at the bottom of the bioreactor support (15) for supporting the bioreactor (3) The weight of the reactor 3 is measured. The weight change of the culture plant is sprayed at regular intervals through the medium injection nozzle (6) installed inside the bioreactor (3) using a medium circulation pump (8) the medium of the medium storage tank (7) After recovering the injected medium to the medium storage tank (7) it can be obtained by measuring the weight of the bioreactor using the load cell (4). The weight of the cultured plant measures the continuous weight change of the bioreactor (3), and subtracts the weight of the bioreactor (3) in the absence of the previously measured plant from the measurement, and then the actual cultured plant's own weight. can do.

Since the load cell 4 continuously outputs a weight value as an electric signal, the weight of the bioreactor 3 can be continuously measured. The weight value of the output electrical signal is input to the computer 14 by an interface using an A / D converter.

Figure 4 is a perspective view showing a medium injection of the medium injection plant culture system according to the present invention. Bioreactor (3) for plant culture according to the present invention is a cylindrical container having a medium outlet (17) for the discharge of the medium, an air inlet (18) and an air outlet (19) for the inlet and discharge of external air, At each air inlet, a pollution prevention air filter 10 is provided. Inside the bioreactor 3, a culture support 5 made of stainless steel is installed, and a medium injection nozzle 6 is installed at the bottom of the culture support 5 to supply the medium to the top of the culture support 5. do. The inside of the bioreactor (3) is divided into a culture space, the lower portion of the culture support, the medium injection space, and the culture plant is sealed so as not to move from the top to the bottom. If necessary, the quantity and position of the lower end of the medium dispensing nozzle 6 can be arbitrarily changed or adjusted. In addition, the interval between the medium injection interval and the time until the start of measurement after the medium injection can be flexibly adjusted according to the type of plant and growth stage.

The medium is circulated between the medium reservoir 7 and the bioreactor 3 during the culture period. When the medium circulation pump 8 is operated, the medium is sucked from the medium storage tank 7, moved into the bioreactor 3 through the tube 12, and injected into the medium injection nozzle 6 installed in the tube 12. do. The injected medium is discharged to the medium discharge port 17 installed at the bottom of the bioreactor 3 and recovered to the medium storage tank 7 through the tube 12. The pH of the recovered medium is measured by a pH meter 9 installed inside the medium storage tank 7. Air is introduced into the bioreactor 3 through the air inlet 18 to which the air filter 10 is attached by the air circulation pump 11, and through the air outlet 19 to which the air filter 10 is attached. Discharged.

In the present embodiment, the A / D converter described above, the artificial light source 13 installed inside the main culture chamber 1, the temperature control device, and the pH meter 9 installed in the medium storage tank 7 are commonly used in the related art. Since the device is a detailed description of the device will be omitted.

The control unit 2 is provided with a microcomputer that is attached to the inside of the culture chamber 1 and transmits various control signals based on the values measured from each measuring device, and a setting operation unit for setting the internal environmental conditions and the control type. In addition to the culture chamber 1, it is connected to a computer (PC) 14 capable of controlling the culture chamber 1 directly from the outside and a measurement / control cable 16.

The control pattern of the plant culture system is divided into set point control and computer control, and the control type can be switched using a switch. For example, in the set value control mode, all the control is controlled based on the value input to the setting operation unit attached to the main culture chamber 1, and in the computer control mode, all the control is controlled by the external computer 14. All the measured data by computer control mode can be monitored in real time through the measurement / control cable 16, and the user-developed control program can be applied.

Items to be controlled by the control unit 2 include temperature, light intensity, air circulation time, medium injection time, and weighing time. According to a preferred embodiment of the present invention, the temperature range of the controlled object is 10-25 ° C., the light intensity is 500-10,000 lx, and the air circulation time, the medium injection time, and the weighing time may vary depending on the target crop and the growth stage. .

On the other hand, the plant culture system, the medium injection method and the continuous weighing method of the culture plant according to the present invention is not limited only to the above embodiment, the scope of the present invention by installing a culture support in the bioreactor medium from the bottom of the culture support Injecting, and includes all culture methods and devices configured to enable continuous weighing of the culture plants using a load cell.

As described above in detail with reference to the preferred configuration of the present invention, the present invention is a good environment for the growth of culture plants by increasing the exposure time of the culture plants to the breeding season using the medium injection method By continuously measuring the weight of the cultured plant using a load cell, it is possible to measure the growth rate of the cultured plant according to the environmental conditions and to control the proper growth environment of the cultured plant based thereon. It is an industrially useful invention.

Claims (4)

A culture chamber 1 which protects the bioreactor 3 during the culturing process and provides a culture environment; A control unit (2) attached to the culture chamber (1) for transmitting a control signal according to a set value and a control form input from each device; A plant culture bioreactor (3) installed inside the culture chamber (1); A bioreactor supporter 15 installed at the bottom of the bioreactor 3 to support the bioreactor 3; A load cell (4) installed at the bottom of the bioreactor supporter (15) to measure the weight of the bioreactor (3); A culture support (5) installed inside the bioreactor (3) to support the culture plants; Medium dispensing nozzle (6) installed at the bottom of the culture support (5); Medium storage tank (7) for storing the medium for plant culture; A medium circulation pump (8) for supplying or recovering the medium in the medium storage tank (7) to the bioreactor (3); A pH measuring device 9 installed inside the medium storage tank 7 to measure pH of the medium; An air filter 10 for preventing contamination of the culture plant; An air circulation pump 11 for introducing or discharging external air into the bioreactor 3; A tube 12 which is a passage of medium and air; Artificial light source 13 which is an energy source of culture plants; A computer 14 connected to the control unit 2 and the measurement / control cable 16 to directly control the system from the outside; A medium injection plant culture system capable of continuous weighing of a culture plant, characterized in that it comprises a medium outlet (17) and an air inlet / outlet (18, 19). [Claim 2] According to claim 1, Media control plant culture system capable of continuous weighing of the culture plant, characterized in that it comprises the temperature, light intensity, air circulation time and medium injection time which is an item controlled by the control unit (2) . The medium-jet plant culture system of claim 1, wherein the control mode is arbitrarily selected from a setpoint control or a computer control. The bioreactor 3 is divided into an upper space and a lower space with a stainless steel culture support 5 installed on the inside of the bioreactor 3, and the plants are cultured on the upper portion of the culture support 5. Supplying the medium to the culture plant by spraying the medium onto the culture support (5) using a medium injection nozzle (6) and a medium circulation pump (8) installed in the space; After a certain period of time, the supplied medium is discharged from the bioreactor (3) and recovered to the medium storage tank (7), and then the bioreactor (3) is changed using a load cell (4) installed at the bottom of the bioreactor (3). Continuous weighing method of the culture plant, characterized in that it comprises the step of measuring the weight change of the culture plant by measuring the weight of).