KR0160336B1 - The method of cultivating mushrooms - Google Patents

Abstract

The present invention relates to a method of artificial inoculation of pine bacteria by using a soil environment composition agent for creating a soil environment suitable for the growth of pine mycelium as the main material and a viability promoting agent that can increase the viability of the hyphae.

The research on artificial inoculation method of pine mushroom has been conducted in various ways, but the results have not been achieved due to problems. Among these methods, the inoculation method using mycelial seedlings was not able to supply smooth moisture to pine trees after planting due to the water splashing phenomenon of hyphae attached to pine roots. The lack of hyphae infected by lack of growth does not occur, the phenomenon appears. Another method is the mycelial phagocytosis method, which collects underground mycelia from the existing pine area without inoculating pine seedlings and inoculates the inoculation site. Due to the lack of capacity, the inoculated hyphae do not grow and disappear.

Therefore, the inventors of the present invention in order to solve this problem, the water content of the soil before inoculation to the level suitable for mycelial growth (about 30%) and then inoculated with mycelium and reinforces the nutrient source of the mycelium to increase the viability of the mycelium water A new artificial inoculation method has been developed to improve the ability to fry to significantly increase the inoculation rate.

First, the soil environment composition agent (1 L of alcohol with a concentration of 60% or more, 50-90 g of rosin, 0.1-0.5 g of tannin, and 0.1-0.5 g of phosphorus) was removed and sprayed after removal of the indicator so that the well-developed pine roots appeared. By replacing the weak water splashing ability of the hyphae with the environmental composition agent, it is possible to maintain the soil moisture content even in rainy weather. Inoculate the prepared underground mycelia to the center of the prepared inoculation paper, cover the soil about 5cm, and then put on mycelial growth promoter (60-90g of rosin 1L alcohol, 60-90% concentration, protein (legumin) 100-300mg, chestnut honey 10) Diluted solution of diluted solution of 5 ~ 10 ml of mixed solution of 20 ~ 20 ml) 3 times at intervals of 3 to 4 months to promote mycelial growth. Inoculation rates of more than% can be obtained.

Description

Artificial inoculation method of pine hyphae based on rosin

The present invention relates to a method of artificial inoculation of pine mushrooms using a rosin as a main ingredient and to develop a soil environment composition agent for increasing the soil environment suitable for the growth of pine hyphae, and to increase the viability of hyphae. The research on the artificial inoculation method of Song Yi-kyun has been tried variously for a long time, but it is not practically applied. Among these methods, the use of cluster spores made a mixture of water and spores, sprayed them on forest land, and collected spores to make dumplings to inoculate the tips of pine roots. In addition, by using fungal seedlings, young pine seedlings are planted in natural occurrences of cluster mycelia, or cultured in test tubes, seedlings are introduced, seedlings of pine trees are formed on them to form mycorrhizal fungus and transferred to forest land. After planting, it was officially announced in Japan that a single inoculation method was attempted to cause a second infection on large pine roots, but the inoculation method using fungal seedlings is the mycelial water attached to the pine roots. Due to the frying phenomenon, the pine tree is not supplied with smooth moisture after planting, and thus the initial growth rate of the pine tree is low, and the hyphae of the poor hyphae lacks the water splashing ability. On the other hand, there is a mycelial phagocytosis method that inoculates the inoculation site by filling underground mycelium (shiro) from the existing pine where the pine is not produced. The problem of this method is that the mycelia of the collected underground mycelia are weak and the water splashes. Due to the lack of capacity, the inoculated hyphae do not grow and disappear. The reason why the second forest vaccination is very difficult is that the mycelial watering ability, which is one of the main factors in the mycelial growth, is weakened due to the weak strength of the hyphae at the initial stage of inoculation. Therefore, in order to solve this problem, it is possible to maintain the water content of the inoculated soil at around 30% in the pre-inoculation stage, to replace the water splashing ability of weak hyphae, and to reinforce the mycelium's nutritional source to enhance the hyphae's viability. This is essential.

The inventors of the present invention have been able to significantly improve the artificial inoculation rate by more than 50% by developing an accelerator and an artificial inoculation method using the same to increase the viability of mycelia while maintaining a constant moisture content of the soil using pine resin. Therefore, this promotion can be used to extend the natural benefits of Woori River to Songsan region, and it will contribute to the income of forestry farmers and the national economy by increasing the harvest of Songi, one of the major export products today. . Hereinafter will be described in detail the manufacturing process of the promoter of the present invention and artificial inoculation method of Matsutake bacteria using the same.

[Step 1 (Soil Environment Composition Agent and Environment Composition Process)]

1 to 1 liter of alcohol, 60% or more of high pine extract extracted from red pine, 0.1 to 0.5g of tannin, 0.1 to 0.5g of phosphorus are mixed, sealed and placed at room temperature to completely dissolve and have a sugar content of 32 or more. Soil environment composition) is made. Soil environment composition is a pale yellow transparent liquid that is highly bactericidal. By selecting the site of inoculation, the topsoil is removed so that well-developed pine roots appear on the surface. When the water content of the soil is reduced, water splashing phenomenon such as pine mycelium soil occurs, and during rainfall, it prevents the penetration of water and, after 2 to 3 months, creates a soil environment of about 30% water content suitable for growth of pine mycelia. .

[2nd process (mycelial growth promoting agent and inoculation process)]

In the same manner as in the first step, the mixture of 50 to 90 g of high pine extract extracted from red pine, 10 to 20 ml of chestnut honey, and 100 to 300 mg of protein (legumin) is mixed in 1 liter of alcohol having a concentration of 60% or more in a closed container to make a high-stock stock solution. 5-10 mL is mixed with 1 L of water again to form a diluent (hereinafter referred to as mycelial growth promoter). Mycelial growth promoter is a milky white solution with a sugar content of 3% and pH 6.0, similar to pine sap. Underground mycelial soil as a seed, put it in the center of the inoculation site where the soil environment was formed as described in the first step, cover the soil to about 5 cm, and then apply enough mycelial growth promoting agent, and then step on the foot. Three months after the application of the accelerator is the period of mycelial formation during mycelial growth. After three months of the first spraying, the mycelial growth promoting agent is sprayed in the same manner as the first, and the bacterium is smoothly formed to maintain the viability and grow. After 3 to 4 months of secondary spraying, the mycelial growth booster is widely applied to the inoculation site to promote growth, and after 1 year, fungi of about 10cm diameter are formed. In addition, if the mycelial power is sprayed on the weakened force of the mycelial mycelia, the power of the mycelium is active again.

[3rd step (Step spawn preparation process using mycelial growth promoter)]

Prepare a plastic cylinder (4cm in diameter, 5cm in height) without a lid to find naturally occurring clusters of fungi, remove the surface of the central part where fruiting bodies do not occur, and collect the ground fungus soil and put it in the prepared container. Soak it for about a minute, and bury it in the sand with a low moisture content (less than 30%). In addition, the ground mycelial soil is collected and then covered with a mycelium viability promoter to cover the ground mycelial soil after 1-2 years.

[4th process (checking the inoculation of mycelia and re-injecting seed)]

After inoculation three times after the inoculation stated in the second step after the application of mycelial viability promoter 9 to 10 months after the rain and check if you can easily check whether the inoculation. The inoculation area is well splashed and the inoculation site is dry, and the finger is pressed to give elasticity, and blown into the mouth to remove the grains. Where there is no inoculation, on the contrary, there is a lot of water in the ground and no mycelia are found. Here, after inoculating the spawn prepared in the third step, the inoculation rate can be increased by spraying the mycelial growth promoter.

EXAMPLE

As shown in Table 1, the conventional method is hardly inoculated with the hyphae, even if it is made, the growth rate of the fungus is very slow, it takes a long time until the fruiting body occurs. In addition, the existing method is very limited in the choice of inoculation site. In other words, the soil moisture content and sunshine should be carefully considered. However, the new method of the present invention can increase the inoculation rate by 50% or more and can also exert flexibility in the selection of the inoculation site. In addition, the growth rate of the hyphae is also fast, the fruiting body first development period can be seen to be significantly reduced.

According to (Table 2), the soil environment composition period varies according to the soil moisture content of the inoculation site. In general, when the slope is not steep and the bush is not overly plentiful, the moisture content of 40% to 50% may create a soil environment suitable for mycelial growth in the shortest period.

According to (Table 3), the inoculation rate of hyphae varies depending on the temperature, and generally shows the best inoculation rate between 1 ℃ and 10 ℃.

Seasonal analysis of mycelial inoculation rates in Table 4 shows that the inoculation rates are highest during late winter, early spring, especially around February, when temperatures are within 10 ° C on average. Therefore, according to the above table, the new artificial inoculation method of the present invention, if the inoculation is carried out around February when the temperature is generally within 10 ° C, the inoculation rate is greatly increased without being greatly affected by the selection of the inoculation site, By replacing the weak mycelium's water splashing ability and strengthening the nutrient source, the mycelium's strength is strengthened, the growth rate of the fungus can be increased and the expected period of fruiting can be greatly shortened.

Claims (1)

Before inoculating spawn (artificial bacterium), apply 50 ~ 150 mL of soil environment composition to the inoculation site to adjust the water content of soil to about 30% suitable for mycelial growth. After inoculating spawn at 10 ℃, cover the soil to about 5cm, and apply 50ml-150m of mycelial viability promoter per pyeong on it to reinforce water splashing ability and nutrient source to intensify the viability of mycelia to increase the inoculation rate. 2. The mixed solution according to the above 1, wherein tansin (0.1-0.5 g), phosphorus (0.1-0.5 g) and 1 L of water are mixed in a solution of rosin (50-90 g) dissolved in 1 L of alcohol (concentration of 60% or more). Inoculation method to raise the inoculation rate of the pine mushroom using a rosin-based soil environment composition agent). 3. The method according to the above 1, wherein 5-10 mL of a mixture of protein (100-300 mg) and chestnut honey (10-20 mL) is diluted with 1 L of water in a solution of rosin (60-90 g) dissolved in 1 L of alcohol (concentration of 60% or more). Inoculation method to raise inoculation rate of Matsutake bacteria using one solution (mycelium viability-promoting agent with rosin as main ingredient).