KR0152738B1 - Antioxidants and chromosomal disorder inhibitors containing the extracts of ecklonia stolonifera - Google Patents

Abstract

The present invention relates to an antioxidant and chromosomal aberration inhibitors of gompi extract. More specifically, the methanol extract of gompi extract has antioxidant activity, free radical scavenging activity and chromosomal aberration inhibitory activity. It is about.

Gokpi (Ecklonia stolonifera) is a brown algae belonging to the kelp family that grows on rocks within 10m of depth and is used for edible in civilian.

The present invention is to examine the antioxidant effect on the lipid peroxidation of methanol extract extracted from gompi methanol and its fractions by TBA assay, and also 1,1-diphenyl-2-picrylhydrazil (1,1 -diphenyl-2-picry1hydrazy (DPPH)) The free radical scavenging effect on radicals was measured. The methanol extract of Gompi showed antioxidant activity against lipid peroxidation and free radical scavenging. Chromosomal Abnormality Inhibition Experimental results show that the methanol extract of Gompi confirms the effect of inhibiting chromosomal abnormality by benzo (a) pyrene and provides the use for the prevention of aging and the prevention of diseases caused by chromosomal abnormalities.

Description

[Name of invention]

Antioxidant and Chromosome Abnormal Inhibitor with Gompi Extract

Detailed description of the invention

The present invention relates to an antioxidant and chromosomal aberration inhibitors of gompi extract. More specifically, the methanol extract of gompi extract has antioxidant activity, free radical scavenging activity and chromosomal aberration inhibitory activity. It is about.

Gokpi (Ecklonia stolonifera) is a seaweed belonging to the seaweed of seaweed, which belongs to the seaweed, which grows on rocks within 10m of water, and has been used for food for a long time. . In the Gompi, phlorotannin and eclonialactone, such as phloroglucinol, have been isolated and reported. Although there have been reports of anti-cancer effects on Sarcoma-180 cells and studies on antioxidant activity and chromosomal aberration in other seaweeds, there are studies on the antioxidant activity of gompi and the inhibition of chromosomal aberration. Very little research has been done. Therefore, it is an object of the present invention to identify the use of gompi extract to provide food or pharmaceutical use.

The present inventors have tested the antioxidant effect on the lipid peroxidation of gompi methanol extract by TBA assay, and also 1,1-diphenyl-2-picrylhydrazyl (1,1-dipheny1-2picry1hydrazy (DPPH) As a result of free radical scavenging effect on radicals, the methanol extract of Gompi showed antioxidant activity on lipid peroxidation and scavenging of free radicals. Blood methanol extract inhibited about 80% of chromosomal aberration caused by benzo (a) pyrene.

Thus, the methanol extract of gompi of the present invention has useful activity for antioxidant activity and chromosomal aberration inhibitory activity, and thus, it is useful for use as an anti-aging and chromosomal ailment prevention agent of the body by such activity.

For the purpose of treatment, the extract of the present invention and its pharmaceutical salts are mixed with a suitable carrier such as an organic or inorganic, solid or liquid excipient suitable for oral, injection or medicament to contain the extract as an active ingredient. It can be used as a form of preparation. Pharmaceutical formulations can be made with capsulants, tablets, dragees, ointments, granules, solutions, suspensions, emulsions, injections and the like.

If necessary, these preparations can include auxiliary substances, stabilizers, wetting or emulsifying agents, buffers and other commonly used additives.

The dose of the extract of the present invention can be changed according to the age of the patient, but an effective amount of 1 mgmg, 2mg, 5, mg 10mg, 20mg, etc. can be used, and more than 1mg is effective for treatment.

Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited to these Examples.

Example 1

[Method of Preparing Methanol Extract of Gompi]

Methanol extract of Gompi was extracted three times at room temperature with 100% methanol using fine dried Gompi (2.9 kg) as a sample. The extract was filtered and concentrated under reduced pressure to obtain a methanol extract of gompi (500 g). The final yield of the methanol extracts of these Gompi is shown in Table 1 below.

Example 2

Antioxidant Application

1. TBA assay

The antioxidant activity of methanol extract from gompi was measured by TBA assay using 2-thiobarbituric acid (TBA) for lipid peroxidation of mouse liver homogenate. That is, 0.3 ml of mouse liver lysate and 0.3 ml of 8.1% SDS solution and 0.1 ml of sample solution were added to a test tube and reacted at 37 ° C. for 6 hours. Next, 1.5 ml of 20% acetic acid and 1 ml of 1.2% TBA solution were added to the reaction solution and then boiled at 100 ° C. for 30 minutes. Next, the reaction solution was cooled to room temperature, centrifuged at 2500 rpm for 15 minutes, and the absorbance of the supernatant was measured at 532 nm to obtain an antioxidant effect.

The absorbance value of 0.1 was defined as 1 TBA unit and the degree of lipid peroxidation was expressed as TBA value per g of liver weight. The measurement results of this antioxidant effect are shown in Table 2 below.

As can be seen in Table 2, the methanol extract of Gompi has an antioxidant effect on lipid peroxidation of liver decay products at 10 mg / ml.

2. Elimination effect of free radical

The measurement of the pyripadical scavenging effect of the methanol extract of Gompi was carried out using 1 ml of 1,1-diphenyl-2-picrylhydrazyl (1.5 × 10) After adding 1 ml of M) methanol solution, the mixture was left at room temperature for 30 minutes, and then absorbance at 520 nm was measured to determine the free radical scavenging action. The measurement results are shown in Table 3 below.

As can be seen in Table 3, the methanol extract of Gompi shows strong scavenging effect on free radicals and is dependent on the concentration.

Example 3

[Inhibition of Chromosome Aberration]

In vitro chromosome aberration inhibitory activity of methanol extract of gompi was measured as follows. In other words, Chinese hamster lung cells (Chinese hamster lung cells) in 5ml medium initial cell concentration 1 × 10 It is grown in a Petri dish having a diameter of 60 nm in / ml. After injecting each concentration sample to the cells grown for one day, 1 ml of S9 mix (S9 mix) is added. After 6 hours of incubation, replace with new medium and incubate another 18 hours so that the total incubation time is 24 hours. After 22 hours of incubation, colcemid was added and cultured for 2 hours. The cells were harvested and observed for chromosomal abnormalities. In this case, benzopyrene (benzo (a) pyrene) is used as a positive control to obtain a chromosome abnormality inhibiting activity (%) of the sample.

As shown in Table 4, the methanol extract of Gompi showed inhibitory effect on chromosomal aberration caused by benzopyrene at 125 µg / ml and 250 µg / ml.

As shown in the above examples, the methanol extract of gompi according to the present invention exhibits strong antioxidant activity, free radical scavenging effect and chromosomal aberration inhibitory activity, and is used as a therapeutic agent for oxidative damage supported by these activities or as a therapeutic agent for chromosomal abnormalities. It is useful.

Claims (2)

An antioxidant and free radical scavenging agent, characterized in that isomer extract, excipient and other pharmaceutically acceptable additives may be added as a main ingredient. A chromosomal abnormality inhibitor, characterized in that isomer extract, excipient and other pharmaceutically acceptable additives can be added to the extract from the dried gompi sample with methanol.