KR0140953B1 - Preparation process of bacteriocide containing organic copper - Google Patents

Abstract

A liquid disinfectant that dissolves citric acid and equivalents in ion-exchanged water and improves solubility, and relates to a method for producing a copper organic disinfectant having excellent long-term storage and electrodeposition properties.

Description

Manufacturing method of fungicide of organic copper

The present invention relates to a method for preparing an organic copper disinfectant containing copper sulfate and citric acid as a main component, and more particularly to a method for preparing a blue blue clear liquid disinfectant in which copper sulfate pentahydrate and citric acid are dissolved in water as an excipient.

As a fungicide of the inorganic copper agent known in the art, the brood solution is well known to be effective for downy mildew, and its composition is made of lime oil by dissolving 80-90% copper sulfate and 10-20% of hydrated lime in water. Although it is a disinfectant, if it is left for a long time, blue sediment will settle on the bottom of the container, and it will be separated into clear water on the bottom, which makes it difficult to store for a long time. There is a drawback that there is a tendency to do so and the range of use is not wide.

In addition, as an inorganic copper fungicide, basic copper sulfate [CuSO ₄ · Cu (oH)] ₂ , basic copper chloride [CuCl ₂ · 3Cu (oH) ₂ ], and basic copper phosphate [Cu (DO ₄ ) X (SO ₄ ) y (OH) ₂ ), yellow zinc sulfide (Cu ₂ O), etc. There are fatty acid copper sulfur etc. available, but these copper compounds are not soluble in water, and the method of electrodepositing and fixing fine copper compound particles to the sterilized crop is problematic. And physical properties are also poor.

In addition, as an organic copper disinfectant, its active ingredient is 80-hydroxyhino quinoline copper, which is a yellow crystal, which has a chemical structure in which copper ion chelates with ozine. It does not happen well, but it has a high fish toxicity and disadvantages of adding an electrodeposition agent separately.

The present invention is to solve the problems of the conventional copper fungicides as described above is a wide range of application, there is no weakness, there is no crystallization and extremely excellent preservation as a fungicide is as follows.

First, to obtain a dilute citric acid solution and water were added to the citric acid can be called in which maintaining the temperature of yiyongaek exactly 50 ℃ -60 ℃ and then copper sulfate (CuSO ₄ · 5H ₂ O) was added to the process for producing a disinfectant obtained because dissolution standing .

What is important in the present invention is the composition ratio of the components and the order of dissolution of the composition, and particularly important is the use of ion exchanged water as excipient. Here, the composition of the composition is composed of 20% copper sulfate, 10% citric acid, and 70% ion-exchanged water by weight, and the dissolution order of the composition is obtained by adding ion-exchanged water to citric acid to obtain a diluted citric acid solution, and adding copper sulfate to the solution. It is a means to make it.

If this step is out of order, the disinfectant solution of the finished product is immediately separated from the crystal of copper sulfate and the dilute solution of citric acid, and more severely separated when using distilled or natural water other than ion-exchanged water. After all, the feature of the method is that the composition constituting the fungicide solution according to the present invention, the dissolution order of the composition and the water used as an excipient use ion-exchanged water.

In the above method, polar ion exchange water is used, so that the dissolution rate is fast, crystal precipitation does not occur at all in the finished product, and citric acid is added, so that dispersibility and electrodeposition are superior to those of hydroxy quinone copper, which is a fungicide of organic copper. There is no need to add an additional electrodeposition agent. In addition, there are gas removal functions such as SO ₂ and anti-oxidation function to prevent the disinfectant from disinfecting.

In addition, when 20% or more of copper sulfate is used in the composition ratio, crystallization easily occurs, and when it is used below, sterilization power is lowered.

The action of the solution disinfectant prepared according to the present invention is to prevent and treat the occurrence of fish disease by the selective sterilization of aquatic microorganisms. By promoting the decomposition of water, BOD, COD, SS, etc. of the water quality can be improved and the toxicity of eutrophication sediment can be removed.

In addition, it has excellent sterilizing power and excellent ability to decompose algae and organic matter and has a selective sterilizing ability. Therefore, harmful bacteria are killed and harmless to beneficial bacteria, and it is economical because of its wide range of uses and high dilution ratio. In addition, the long-term preservation of the product is excellent, and the proper magnification is harmless to the human body of animals and plants, and the skin and toxicity level is harmless as 3rd grade (cola level).

In addition, the dilution capacity of water suitable for the place of use and place of use is shown in Table 1 below.

In the above, the function and action, properties, uses, etc. of the solution disinfectant according to the present invention are mentioned, but the examples are shown for clarity.

(Example 1)

(1) Test object: Escherichia coil (YUFE2046)

(2) Culture medium of E. coli

1) Juicy medium (solution medium)

Fefton .......... 10g

Juicy .......... 10g

Sodium Chloride .......... 5g

Glucose ..... 5g

PH (adjust with NaoH) ........... 7.0-7.3

The material was completely dissolved in 1000 ml of purified water and then 121 ° C./15-20 minutes at sterile 1.2 kg / cm 2 pressure.

2) Juicy agar medium (fixed medium)

15 g (20 g in summer) of agar refined powder was added to the broth of 1).

3) Experiment

E. coli (Escherichia coil YUFE2046) was picked from a slant preserved with platinum and inoculated in a solution medium (1) in the test tube, incubated at 32 ℃ -35 ℃ for 36 hours, and then plated (fixed). Pipette 1 ml of the E. coli culture onto the surface 2) of the medium (2) with a pipette and evenly disperse with a round glass dispersion rod.

Separately refined white surface was diluted with a solution disinfectant of 1 m / / 1000 = 0.1% according to the present invention and treated for 40 minutes at 50 ° C. for drying for 40 minutes, and then cut into a piece of cloth and adhered to the flat plate. Then, after 24 hours at 35 ° C., the colonies of bacteria were observed. As a result, the part where the short specimen was in close contact was sterilized, and the low-ring ring was produced by leaching of the disinfectant around the short cloth.

In addition to the above (Example 1), excretion liquid of bacteria (Klebsiela pneumonae ATCC 100) that generates odor and ammonia gas, excretion liquid of bacteria (salmonella cholera ATCC 10708) found in manure, and bacteria contained in general wastewater (psedonas aerugrnosa) Examples of the culture medium, bacterial food poisoning and purulent diseases and decayed bacteria as a culture solution of Staphylococcus ATCC 65381 (staphylococcus ATCC 65381) was the same experiment as in Example 1 resulted in almost the same results.

(Example 2)

As a test for growing water (green algae), samples of green algae were sampled in nature, and glucose and peptone were administered as 0.5% nutrients in the stream, respectively, and the incubator was incubated for 25 days under the sun and propagated. After adding to 800ppm container of this disinfectant solution, the growth condition was observed and the green algae were killed and showed a clean state.

Claims (1)

Dissolving 10% citric acid in 70% of ion-exchanged water by weight ratio to obtain a dilute citric acid solution and maintaining the temperature of the solution at 50-60 ° C. and dissolving 20% copper sulfate therein. .