JPWO2022236147A5 - - Google Patents
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- JPWO2022236147A5 JPWO2022236147A5 JP2023568340A JP2023568340A JPWO2022236147A5 JP WO2022236147 A5 JPWO2022236147 A5 JP WO2022236147A5 JP 2023568340 A JP2023568340 A JP 2023568340A JP 2023568340 A JP2023568340 A JP 2023568340A JP WO2022236147 A5 JPWO2022236147 A5 JP WO2022236147A5
- Authority
- JP
- Japan
- Prior art keywords
- composition
- nls
- sequence
- cell
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 47
- 108090000765 processed proteins & peptides Proteins 0.000 claims 38
- 229920001184 polypeptide Polymers 0.000 claims 36
- 102000004196 processed proteins & peptides Human genes 0.000 claims 36
- 101710163270 Nuclease Proteins 0.000 claims 30
- 210000004027 cell Anatomy 0.000 claims 25
- 108091033319 polynucleotide Proteins 0.000 claims 20
- 102000040430 polynucleotide Human genes 0.000 claims 20
- 239000002157 polynucleotide Substances 0.000 claims 20
- 108091033409 CRISPR Proteins 0.000 claims 17
- 238000010354 CRISPR gene editing Methods 0.000 claims 17
- 238000003776 cleavage reaction Methods 0.000 claims 14
- 238000000034 method Methods 0.000 claims 14
- 230000007017 scission Effects 0.000 claims 14
- 238000000746 purification Methods 0.000 claims 10
- 102000002488 Nucleoplasmin Human genes 0.000 claims 8
- 108060005597 nucleoplasmin Proteins 0.000 claims 8
- 101710128836 Large T antigen Proteins 0.000 claims 7
- 239000002773 nucleotide Substances 0.000 claims 7
- 125000003729 nucleotide group Chemical group 0.000 claims 7
- 108020005004 Guide RNA Proteins 0.000 claims 6
- 101710135898 Myc proto-oncogene protein Proteins 0.000 claims 6
- 102100038895 Myc proto-oncogene protein Human genes 0.000 claims 6
- 101710150448 Transcriptional regulator Myc Proteins 0.000 claims 6
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 5
- 101100011365 Caenorhabditis elegans egl-13 gene Proteins 0.000 claims 4
- 102000005720 Glutathione transferase Human genes 0.000 claims 4
- 108010070675 Glutathione transferase Proteins 0.000 claims 4
- 210000001744 T-lymphocyte Anatomy 0.000 claims 4
- 241000723792 Tobacco etch virus Species 0.000 claims 4
- 238000010362 genome editing Methods 0.000 claims 4
- 229920006318 anionic polymer Polymers 0.000 claims 3
- 210000005260 human cell Anatomy 0.000 claims 3
- 210000002865 immune cell Anatomy 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 210000000130 stem cell Anatomy 0.000 claims 3
- 101100011368 Caenorhabditis elegans egl-1 gene Proteins 0.000 claims 2
- 102000000584 Calmodulin Human genes 0.000 claims 2
- 108010041952 Calmodulin Proteins 0.000 claims 2
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 claims 2
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 claims 2
- 101100078999 Mus musculus Mx1 gene Proteins 0.000 claims 2
- 102000012338 Poly(ADP-ribose) Polymerases Human genes 0.000 claims 2
- 108010061844 Poly(ADP-ribose) Polymerases Proteins 0.000 claims 2
- 229920000776 Poly(Adenosine diphosphate-ribose) polymerase Polymers 0.000 claims 2
- 108010020346 Polyglutamic Acid Proteins 0.000 claims 2
- 206010039491 Sarcoma Diseases 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 241000700605 Viruses Species 0.000 claims 2
- 102000009899 alpha Karyopherins Human genes 0.000 claims 2
- 108010077099 alpha Karyopherins Proteins 0.000 claims 2
- 239000000427 antigen Substances 0.000 claims 2
- 102000036639 antigens Human genes 0.000 claims 2
- 108091007433 antigens Proteins 0.000 claims 2
- 238000007385 chemical modification Methods 0.000 claims 2
- 238000004520 electroporation Methods 0.000 claims 2
- 239000003862 glucocorticoid Substances 0.000 claims 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 2
- 208000006454 hepatitis Diseases 0.000 claims 2
- 231100000283 hepatitis Toxicity 0.000 claims 2
- 108700032552 influenza virus INS1 Proteins 0.000 claims 2
- 230000007246 mechanism Effects 0.000 claims 2
- 230000030648 nucleus localization Effects 0.000 claims 2
- 229920002643 polyglutamic acid Polymers 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 125000006850 spacer group Chemical group 0.000 claims 2
- 108020003113 steroid hormone receptors Proteins 0.000 claims 2
- 102000005969 steroid hormone receptors Human genes 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 1
- XUYJLQHKOGNDPB-UHFFFAOYSA-N phosphonoacetic acid Chemical compound OC(=O)CP(O)(O)=O XUYJLQHKOGNDPB-UHFFFAOYSA-N 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163185315P | 2021-05-06 | 2021-05-06 | |
| US63/185,315 | 2021-05-06 | ||
| US202263315483P | 2022-03-01 | 2022-03-01 | |
| US63/315,483 | 2022-03-01 | ||
| PCT/US2022/028208 WO2022236147A1 (en) | 2021-05-06 | 2022-05-06 | Modified nucleases |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2024518413A JP2024518413A (ja) | 2024-05-01 |
| JPWO2022236147A5 true JPWO2022236147A5 (https=) | 2025-05-13 |
| JP2024518413A5 JP2024518413A5 (https=) | 2025-05-13 |
Family
ID=81975392
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023568340A Pending JP2024518413A (ja) | 2021-05-06 | 2022-05-06 | 修飾ヌクレアーゼ |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US20260009009A1 (https=) |
| JP (1) | JP2024518413A (https=) |
| CA (1) | CA3218053A1 (https=) |
| WO (1) | WO2022236147A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2024007790A (es) | 2021-12-22 | 2024-09-06 | Camp4 Therapeutics Corp | Modulación de la transcripción génica utilizando oligonucleótidos antisentido dirigidos a arn reguladores. |
| IL324634A (en) | 2023-05-15 | 2026-01-01 | Nchroma Bio Inc | Compositions and methods for epigenetic regulation of hbv gene expression |
| WO2026046724A1 (en) * | 2024-08-30 | 2026-03-05 | Cellectis Sa | Tale protein scaffolds involving fusions of monopartite and bipartite nls |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| WO2017106569A1 (en) * | 2015-12-18 | 2017-06-22 | The Regents Of The University Of California | Modified site-directed modifying polypeptides and methods of use thereof |
| US9896696B2 (en) | 2016-02-15 | 2018-02-20 | Benson Hill Biosystems, Inc. | Compositions and methods for modifying genomes |
| JP7136816B2 (ja) * | 2017-06-23 | 2022-09-13 | インスクリプタ， インコーポレイテッド | 核酸誘導型ヌクレアーゼ |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US10011849B1 (en) * | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US11649442B2 (en) * | 2017-09-08 | 2023-05-16 | The Regents Of The University Of California | RNA-guided endonuclease fusion polypeptides and methods of use thereof |
| WO2019118516A1 (en) * | 2017-12-11 | 2019-06-20 | Editas Medicine, Inc. | Cpf1-related methods and compositions for gene editing |
| EP3821008A1 (en) * | 2018-07-12 | 2021-05-19 | Keygene N.V. | Type v crispr/nuclease-system for genome editing in plant cells |
| AU2019368215B2 (en) * | 2018-10-22 | 2023-05-18 | Inscripta, Inc. | Engineered enzymes |
| WO2020092057A1 (en) * | 2018-10-30 | 2020-05-07 | Yale University | Compositions and methods for rapid and modular generation of chimeric antigen receptor t cells |
| CN113039278A (zh) * | 2018-10-31 | 2021-06-25 | 诺维信公司 | 通过指导的内切核酸酶和单链寡核苷酸进行基因组编辑 |
| EP3887538A1 (en) * | 2018-11-28 | 2021-10-06 | Keygene N.V. | Targeted enrichment by endonuclease protection |
| CA3153700A1 (en) * | 2019-09-09 | 2021-03-18 | Scribe Therapeutics Inc. | Compositions and methods for use in immunotherapy |
| AU2020358863A1 (en) | 2019-10-03 | 2022-05-12 | Celyntra Therapeutics Sa | CRISPR systems with engineered dual guide nucleic acids |
| US20230348869A1 (en) * | 2019-10-14 | 2023-11-02 | KWS SAAT SE & Co. KGaA | Mad7 nuclease in plants and expanding its pam recognition capability |
| CA3166430A1 (en) | 2020-02-05 | 2021-08-12 | Ryan T. Gill | Compositions and methods for targeting, editing or modifying human genes |
-
2022
- 2022-05-06 CA CA3218053A patent/CA3218053A1/en active Pending
- 2022-05-06 WO PCT/US2022/028208 patent/WO2022236147A1/en not_active Ceased
- 2022-05-06 US US18/289,723 patent/US20260009009A1/en active Pending
- 2022-05-06 JP JP2023568340A patent/JP2024518413A/ja active Pending
-
2023
- 2023-04-28 US US18/141,363 patent/US20230340437A1/en not_active Abandoned
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