JPWO2021171688A1 - - Google Patents

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Publication number
JPWO2021171688A1
JPWO2021171688A1 JP2020560506A JP2020560506A JPWO2021171688A1 JP WO2021171688 A1 JPWO2021171688 A1 JP WO2021171688A1 JP 2020560506 A JP2020560506 A JP 2020560506A JP 2020560506 A JP2020560506 A JP 2020560506A JP WO2021171688 A1 JPWO2021171688 A1 JP WO2021171688A1
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JP
Japan
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JP2020560506A
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Japanese (ja)
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pathology (AREA)
  • Mycology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2020560506A 2020-02-26 2020-10-16 Pending JPWO2021171688A1 (https=)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020030740 2020-02-26
JP2020102122 2020-06-12
PCT/JP2020/039169 WO2021171688A1 (ja) 2020-02-26 2020-10-16 遺伝子ノックイン方法、遺伝子ノックイン細胞作製方法、遺伝子ノックイン細胞、がん化リスク評価方法、がん細胞製造方法、及びこれらに用いるためのキット

Publications (1)

Publication Number Publication Date
JPWO2021171688A1 true JPWO2021171688A1 (https=) 2021-09-02

Family

ID=77491351

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020560506A Pending JPWO2021171688A1 (https=) 2020-02-26 2020-10-16

Country Status (5)

Country Link
US (1) US20220112509A1 (https=)
EP (1) EP4112719A1 (https=)
JP (1) JPWO2021171688A1 (https=)
CN (1) CN113574175A (https=)
WO (1) WO2021171688A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114783509B (zh) * 2022-04-21 2024-11-19 桂林医学院附属医院 一种基于RNA靶向结合位点的LncRNA XIST基因敲入动物模型构建方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005003342A1 (ja) * 2003-07-08 2005-01-13 Japan Science And Technology Agency メチル化を利用したトランスジェニック生物を作製する方法およびシステム
WO2015068785A1 (ja) * 2013-11-06 2015-05-14 国立大学法人広島大学 核酸挿入用ベクター
JP2017517250A (ja) * 2014-04-28 2017-06-29 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ．， ＬＬＣ 標的エンドヌクレアーゼを用いる哺乳類ゲノムのエピジェネティック修飾

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6500293B2 (ja) 2015-11-25 2019-04-17 国立大学法人群馬大学 Ｄｎａメチル化編集用キットおよびｄｎａメチル化編集方法
JP6958917B2 (ja) * 2016-08-10 2021-11-02 国立大学法人 東京医科歯科大学 遺伝子ノックイン細胞の作製方法
WO2018035387A1 (en) * 2016-08-17 2018-02-22 The Broad Institute, Inc. Novel crispr enzymes and systems
CN106191116B (zh) * 2016-08-22 2019-10-08 西北农林科技大学 基于CRISPR/Cas9的外源基因敲入整合系统及其建立方法和应用
US20190255106A1 (en) * 2016-09-07 2019-08-22 Flagship Pioneering Inc. Methods and compositions for modulating gene expression
JP6946133B2 (ja) 2017-09-27 2021-10-06 株式会社レナテック がんリスク評価方法及びがんリスク評価システム
US20190225989A1 (en) * 2018-01-19 2019-07-25 Institute of Hematology and Blood Disease Hospital, CAMS & PUMC Gene knockin method and kit for gene knockin
CA3119301A1 (en) * 2018-11-08 2020-05-14 National University Corporation Tokai National Higher Education And Research System Gene therapy employing genome editing with single aav vector

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005003342A1 (ja) * 2003-07-08 2005-01-13 Japan Science And Technology Agency メチル化を利用したトランスジェニック生物を作製する方法およびシステム
WO2015068785A1 (ja) * 2013-11-06 2015-05-14 国立大学法人広島大学 核酸挿入用ベクター
JP2017517250A (ja) * 2014-04-28 2017-06-29 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ．， ＬＬＣ 標的エンドヌクレアーゼを用いる哺乳類ゲノムのエピジェネティック修飾

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KATAYAMA S. ET AL.: "A powerful CRISPR/Cas9-based method for targeted transcriptional activation.", ANGEW. CHEM. INT. ED., vol. 55, JPN6020043676, 2016, pages 6452 - 6456, ISSN: 0005165162 *
KATAYAMA S. ET AL.: "In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated e", LIFE SCIENCE ALLIANCE, vol. 3, no. 1, JPN6020043677, 2019, pages 201900528, ISSN: 0005165163 *
SAKUMA T. ET AL.: "Homologous recombination-independent large gene casette knock-in in CHO cells using TALEN and MMEJ-d", INT. J. MOL. SCI., vol. 16, JPN6020043675, 2015, pages 23849 - 23866, ISSN: 0005165161 *

Also Published As

Publication number Publication date
US20220112509A1 (en) 2022-04-14
CN113574175A (zh) 2021-10-29
WO2021171688A1 (ja) 2021-09-02
EP4112719A1 (en) 2023-01-04

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