JPWO2021108321A5 - - Google Patents
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特定の実施形態が示され、説明されてきたが、上記の説明はこれらの実施形態の範囲を限定することを意図するものではなく、そのような開示は、説明及び例示の目的のみのために提供されることが理解されたい。したがって、以下の特許請求の範囲から逸脱することなく、開示された実施形態に様々な変更及び修正が行われ得る。
上述の実施形態は下記のようにも記載され得るが下記には限定されない。
［構成１］
スライドの面に貼り付けられた検体内に分布する物体の画像を、前記スライドの前記面と非直交角をなす光軸を有する対物レンズを有するカメラを使用して取得する方法であって、前記検体は前記スライド面に対して不均一な高さを有し、
前記検体の第１の直線部分の第１の複数の画像を取得することと、
前記第１の複数の画像に撮像された前記検体の前記直線部分内の物体の焦点を評価することと、
前記第１の直線部分又は前記第１の直線部分とは異なる前記検体の第２の直線部分の第２の複数の画像を取得することと、を含み、前記第２の複数の画像の取得中に、前記第１の複数の画像に撮像された前記物体の前記評価された焦点に基づいて、前記スライド面に対する前記対物レンズの高さを変化させる、方法。
［構成２］
前記第１の複数の画像の取得中に、前記スライド面に対する前記対物レンズの高さがほぼ一定である、構成１に記載の方法。
［構成３］
前記第１の複数の画像に撮像された前記物体の焦点を評価することは、焦点外物体の総数が閾値数を超えるかを決定することを含む、構成１又は２に記載の方法。
［構成４］
前記第１の複数の画像に撮像された前記物体の焦点を評価することは、前記スライド面に対する前記焦点外物体の各々の高さを決定することを含む、構成１から３のいずれか一項に記載の方法。
［構成５］
前記第１の複数の画像における前記物体の焦点を評価することは、前記第１の複数の画像における取得中に、各々の焦点外物体が前記対物レンズの合焦範囲外である前記スライド面に対する高さに位置するかを決定することを含む、構成４に記載の方法。
［構成６］
前記第１の複数の画像における前記物体の焦点を評価することは、前記第１の複数の画像における取得中に、各々の焦点外物体が、前記対物レンズの合焦範囲の最大高さよりも高い又は最小高さよりも低い前記スライド面に対する高さに位置するかを決定することを含む、構成４又は５に記載の方法。
［構成７］
前記第１の複数の画像における前記物体の焦点を評価することは、前記第１の直線部分内の焦点外物体の各々の位置を決定することを含む、構成１から６のいずれか一項に記載の方法。
［構成８］
前記画像取得中に、前記カメラ及び前記スライドの一方又は両方が、他方に対して側面方向に移動する、構成１から７のいずれか一項に記載の方法。
［構成９］
前記スライド面に対する前記対物レンズの高さは、前記直線部分の各々の長さ方向の位置に対する前記カメラの直線位置の関数として、前記カメラに対する前記スライド面の高さを増加及び／又は減少させることによって変化する、構成８に記載の方法。
［構成１０］
前記スライド面に対する前記対物レンズの高さは、前記第１又は第２の直線部分の各々の長さ方向の位置に対する前記カメラの直線位置の関数として、前記スライド面に対する前記カメラの高さを増加及び／又は減少させることによって変化する、構成８に記載の方法。
［構成１１］
前記第２の直線部分から前記第２の複数の画像が取得される、構成１から１０のいずれか一項に記載の方法。
［構成１２］
前記第１の直線部分から前記第２の複数の画像が取得され、前記方法は、
前記第２の複数の画像に撮像された前記物体の焦点を評価することと、
前記第２の直線部分から第３の複数の画像を取得することと、を更に含み、
前記第３の複数画像の取得中に、前記第２の複数の画像に撮影された前記物体の前記評価された焦点に基づいて、前記スライド面に対する前記対物レンズの高さを変化させる、構成１から１０のいずれか一項に記載の方法。
［構成１３］
前記第２の直線部分が前記第１の直線部分に直接隣接している、構成１から１２のいずれか一項に記載の方法。
［構成１４］
前記第１の複数の画像における焦点外物体の総数が前記閾値数を超える場合、前記第１の直線部分から前記第２の複数の画像を取得することと、
前記第１の複数の画像における焦点外物体の総数が前記閾値数を超えない場合、前記第２の直線部分から前記第２の複数の画像を取得することとを更に含む、構成３に記載の方法。
［構成１５］
スライドの面に貼り付けられた検体内に分布する物体の画像を、前記スライドの前記面と非直交角をなす光軸を有する対物レンズを有するカメラを使用して取得する方法であって、前記検体は前記スライド面に対して不均一な高さを有し、前記方法は、
（ａ）前記検体の直線部分の第１の複数の画像を取得することと、
（ｂ）前記第１の複数の画像に撮像された前記物体の焦点を評価することと、
（ｃ）前記検体の同一又は異なる直線部分の第２の複数の画像を取得することと、前記第２の複数の画像の取得中に、前記第１の複数の画像に撮像された前記物体の前記評価された焦点に基づいて、前記スライド面に対する前記対物レンズの高さを変化させ、及び
（ｄ）（ａ）～（ｃ）を検体のほぼ全体の画像が取得されるまで繰り返すこととを含む、方法。
［構成１６］
前記第１の複数の画像における前記物体の前記焦点を評価することは、焦点外物体の総数が閾値数を超えるかを決定することを含む、構成１５に記載の方法。
［構成１７］
前記第１の複数の画像における前記物体の前記焦点を評価することは、前記第１の複数の画像における取得中に、各々の焦点外物体が、前記対物レンズの合焦範囲の最大高さよりも高い又は最小高さよりも低い前記スライド面に対する高さに位置するかを決定することを含む、構成１５又は１６に記載の方法。
［構成１８］
前記第１の複数の画像における前記物体の前記焦点を評価することは、各々の直線部分内の焦点外物体の各々の位置を決定することを含む、構成１５から１７のいずれか一項に記載の方法。
［構成１９］
前記第１及び第２の複数の画像の各々の取得中に、前記カメラ及びスライドの一方が、他方に対して側面方向に移動する、構成１５から１８のいずれか一項に記載の方法。
［構成２０］
前記スライド面に対する前記対物レンズの高さは、各々の直線部分の長さ方向の位置に対する前記カメラの直線位置の関数として、前記カメラに対する前記スライド面の高さを増加及び／又は減少させることによって変化する、構成１９に記載の方法。
［構成２１］
前記スライド面に対する前記対物レンズの高さは、各々の直線部の長さ方向の位置に対する前記カメラの直線位置の関数として、前記スライド面に対する前記カメラの高さを増加及び／又は減少させることによって変化する、構成１９に記載の方法。
［構成２２］
前記第２の複数の画像は前記異なる直線部分から取得され、前記異なる直線部分は前記第１の複数の画像が取得された前記直線部分に直接隣接している、構成１５から２１のいずれか一項に記載の方法。
［構成２３］
スライドの面に貼り付けられた検体内に分布する物体の画像を取得するためのシステムであって、前記検体は前記スライド面に対して不均一な高さを有し、前記システムは、
光軸を有する対物レンズを有するカメラであって、前記光軸が前記スライドの前記面と非直交角をなすように位置決めされた前記カメラと、
前記カメラと動作可能に接続された画像処理部とを備え、前記画像処理部は、
前記カメラによって取得された前記検体の第１の直線部分の第１の複数の画像を受信し、
前記第１の複数の画像に撮像された前記検体の前記直線部分内の物体の焦点を評価し、そして、
前記カメラに、前記第１の直線部分又は前記第１の直線部分とは異なる前記検体の第２の直線部分の第２の複数の画像を取得させ、前記第２の複数の画像の取得中に、前記第１の複数の画像に撮像された前記物体の前記評価された焦点に基づいて、前記スライド面に対する前記対物レンズの高さを変化させる、システム。
［構成２４］
前記第１の複数の画像の取得中に、前記スライド面に対する前記対物レンズの高さはほぼ一定である、構成２３に記載のシステム。
［構成２５］
前記画像処理部は、焦点外物体の総数が閾値数を超えるかを少なくとも部分的に決定することによって、前記第１の複数の画像に撮像された前記物体の前記焦点を評価する、構成２３又は２４に記載のシステム。
［構成２６］
前記画像処理部は、前記スライド面に対する前記焦点外物体の各々の高さを少なくとも部分的に決定することによって、前記第１の複数の画像に撮像された前記物体の前記焦点を評価する、構成２３から２５のいずれか一項に記載のシステム。
［構成２７］
前記第１の複数の画像における取得中に、前記画像処理部は、各々の焦点外物体が前記対物レンズの合焦範囲外にある前記スライド面に対する高さに位置するかを少なくとも部分的に決定することによって、前記第１の複数の画像における前記物体の前記焦点を評価する、構成２６に記載のシステム。
［構成２８］
前記第１の複数の画像における取得中に、前記画像処理部は、各々の焦点外物体が、前記対物レンズの合焦範囲の最大高さよりも高いか最小高さよりも低い前記スライド面に対する高さに位置するかを少なくとも部分的に決定することによって、前記第１の複数の画像における前記物体の前記焦点を評価する、構成２７又は２８に記載のシステム。
［構成２９］
前記画像処理部は、前記第１の直線部分内の焦点外物体の各々の位置を少なくとも部分的に決定することによって、前記第１の複数の画像における前記物体の前記焦点を評価する、構成２３から２８のいずれか一項に記載のシステム。
［構成３０］
前記画像取得中に、前記カメラ及び前記スライドの一方又は両方が、他方に対して側面方向に移動する、構成２３から２９のいずれか一項に記載のシステム。
［構成３１］
前記スライド面に対する前記対物レンズの高さは、前記直線部分の各々の長さ方向の位置に対する前記カメラの直線位置の関数として、前記カメラに対する前記スライド面の高さを増加及び／又は減少させることによって変化する、構成３０に記載のシステム。
［構成３２］
前記スライド面に対する前記対物レンズの高さは、前記第１又は第２の直線部分の長さ方向の位置に対する前記カメラの直線位置の関数として、前記スライド面に対する前記カメラの高さを増加及び／又は減少させることによって変化する、構成３０に記載のシステム。
［構成３３］
前記第２の直線部分から前記第２の複数の画像が取得されることを特徴とする、構成２３から３２のいずれか一項に記載のシステム。
［構成３４］
前記第１の直線部分から前記第２の複数の画像が取得され、前記画像処理部は、
前記第２の複数の画像に撮像された前記物体の焦点を評価し、そして、
前記カメラに、前記第２の直線部分の第３の複数の画像を取得させるように構成され、前記第３の複数の画像の取得中に、前記第２の複数の画像に撮像された前記物体の前記評価された焦点に基づいて、前記スライド面に対する前記対物レンズの高さが変化する、構成２３～３２のいずれか一項に記載のシステム。
［構成３５］
前記第２の直線部分が前記第１の直線部分に直接隣接している、構成２３～３４のいずれか一項に記載のシステム。
Although particular embodiments have been shown and described, the above description is not intended to limit the scope of these embodiments and such disclosure is for purposes of explanation and illustration only. It is understood that this is provided. Accordingly, various changes and modifications may be made to the disclosed embodiments without departing from the scope of the following claims.
The embodiments described above may also be described as follows, but are not limited to the following.
[Configuration 1]
A method for obtaining an image of an object distributed within a specimen affixed to a surface of a slide using a camera having an objective lens having an optical axis making a non-orthogonal angle to the surface of the slide, the method comprising: the specimen has an uneven height with respect to the slide surface;
acquiring a first plurality of images of a first straight section of the specimen;
evaluating a focus of an object within the linear portion of the specimen captured in the first plurality of images;
acquiring a second plurality of images of the first straight line portion or a second straight line portion of the specimen different from the first straight line portion, while acquiring the second plurality of images. The method further comprises changing the height of the objective lens relative to the slide surface based on the estimated focus of the object captured in the first plurality of images.
[Configuration 2]
2. The method of configuration 1, wherein the height of the objective relative to the slide surface is substantially constant during acquisition of the first plurality of images.
[Configuration 3]
3. The method of claim 1 or 2, wherein evaluating the focus of the object imaged in the first plurality of images includes determining whether a total number of out-of-focus objects exceeds a threshold number.
[Configuration 4]
Any one of configurations 1 to 3, wherein evaluating the focus of the object imaged in the first plurality of images includes determining a height of each of the out-of-focus objects relative to the sliding surface. The method described in.
[Configuration 5]
Assessing the focus of the object in the first plurality of images includes, during acquisition in the first plurality of images, each out-of-focus object relative to the slide surface that is outside the focus range of the objective lens. 5. The method of configuration 4, comprising determining whether the height is located at a height.
[Configuration 6]
Assessing the focus of the object in the first plurality of images comprises: during acquisition in the first plurality of images, each out-of-focus object is higher than a maximum height of the in-focus range of the objective lens; or at a height relative to the sliding surface that is lower than a minimum height.
[Configuration 7]
According to any one of configurations 1 to 6, evaluating the focus of the object in the first plurality of images includes determining the position of each out-of-focus object within the first linear portion. Method described.
[Configuration 8]
8. The method according to any one of configurations 1 to 7, wherein during the image acquisition, one or both of the camera and the slide are moved laterally relative to the other.
[Configuration 9]
the height of the objective lens relative to the sliding surface increases and/or decreases the height of the sliding surface relative to the camera as a function of the linear position of the camera relative to the longitudinal position of each of the linear sections; 9. The method according to configuration 8, wherein the method varies according to:
[Configuration 10]
The height of the objective lens relative to the sliding surface increases the height of the camera relative to the sliding surface as a function of the linear position of the camera relative to the longitudinal position of each of the first or second linear sections. and/or the method according to configuration 8.
[Configuration 11]
11. The method according to any one of configurations 1-10, wherein the second plurality of images is acquired from the second straight line section.
[Configuration 12]
the second plurality of images are acquired from the first straight line section, the method comprising:
evaluating the focus of the object captured in the second plurality of images;
obtaining a third plurality of images from the second straight line section;
Configuration 1, wherein during acquisition of the third plurality of images, the height of the objective lens with respect to the slide surface is changed based on the evaluated focus of the object captured in the second plurality of images. 10. The method according to any one of 10 to 10.
[Configuration 13]
13. The method of any one of configurations 1-12, wherein the second straight section is directly adjacent to the first straight section.
[Configuration 14]
obtaining the second plurality of images from the first straight line portion if the total number of out-of-focus objects in the first plurality of images exceeds the threshold number;
and obtaining the second plurality of images from the second straight line portion if the total number of out-of-focus objects in the first plurality of images does not exceed the threshold number. Method.
[Configuration 15]
A method for obtaining an image of an object distributed within a specimen affixed to a surface of a slide using a camera having an objective lens having an optical axis making a non-orthogonal angle to the surface of the slide, the method comprising: The specimen has a non-uniform height with respect to the slide surface, and the method includes:
(a) acquiring a first plurality of images of a straight section of the specimen;
(b) evaluating the focus of the object captured in the first plurality of images;
(c) acquiring a second plurality of images of the same or different linear portions of the specimen; and, during the acquisition of the second plurality of images, of the object imaged in the first plurality of images. changing the height of the objective lens relative to the slide surface based on the evaluated focus; and
(d) repeating steps (a) to (c) until an image of substantially the entire specimen is acquired.
[Configuration 16]
16. The method of claim 15, wherein evaluating the focus of the object in the first plurality of images includes determining whether a total number of out-of-focus objects exceeds a threshold number.
[Configuration 17]
Assessing the focus of the object in the first plurality of images includes determining that during acquisition in the first plurality of images, each out-of-focus object is lower than a maximum height of the focusing range of the objective lens. 17. The method of claim 15 or 16, comprising determining whether to be located at a height relative to the sliding surface that is higher or lower than a minimum height.
[Configuration 18]
18. According to any one of configurations 15 to 17, estimating the focus of the object in the first plurality of images includes determining the position of each out-of-focus object within each straight line segment. the method of.
[Configuration 19]
19. The method of any one of arrangements 15-18, wherein during acquisition of each of the first and second plurality of images, one of the camera and slide moves laterally relative to the other.
[Configuration 20]
The height of the objective lens relative to the sliding surface is determined by increasing and/or decreasing the height of the sliding surface relative to the camera as a function of the linear position of the camera relative to the longitudinal position of each linear section. 19. The method of configuration 19, wherein the method varies.
[Configuration 21]
The height of the objective lens relative to the sliding surface is determined by increasing and/or decreasing the height of the camera relative to the sliding surface as a function of the linear position of the camera relative to the longitudinal position of each linear section. 19. The method of configuration 19, wherein the method varies.
[Configuration 22]
Any one of configurations 15 to 21, wherein the second plurality of images are obtained from the different linear sections, and the different linear sections are directly adjacent to the linear sections from which the first plurality of images were obtained. The method described in section.
[Configuration 23]
A system for acquiring an image of an object distributed within a specimen affixed to the surface of a slide, the specimen having a non-uniform height with respect to the slide surface, the system comprising:
a camera having an objective lens having an optical axis, the camera being positioned such that the optical axis makes a non-orthogonal angle to the surface of the slide;
an image processing section operably connected to the camera, the image processing section comprising:
receiving a first plurality of images of a first linear portion of the specimen acquired by the camera;
evaluating a focus of an object within the linear portion of the specimen imaged in the first plurality of images;
causing the camera to acquire a second plurality of images of the first straight section or a second straight section of the specimen different from the first straight section, and during acquisition of the second plurality of images; , a system for varying the height of the objective lens relative to the slide surface based on the estimated focus of the object captured in the first plurality of images.
[Configuration 24]
24. The system of arrangement 23, wherein the height of the objective relative to the slide surface is substantially constant during acquisition of the first plurality of images.
[Configuration 25]
or configuration 23, wherein the image processing unit evaluates the focus of the object imaged in the first plurality of images by determining, at least in part, whether the total number of out-of-focus objects exceeds a threshold number. The system described in 24.
[Configuration 26]
The image processing unit evaluates the focus of the object captured in the first plurality of images by at least partially determining the height of each of the out-of-focus objects with respect to the slide surface. 26. The system according to any one of 23 to 25.
[Configuration 27]
During acquisition in the first plurality of images, the image processor determines, at least in part, whether each out-of-focus object is located at a height relative to the slide surface that is outside the focus range of the objective lens. 27. The system of arrangement 26, wherein the system evaluates the focus of the object in the first plurality of images by:
[Configuration 28]
During the acquisition of the first plurality of images, the image processing unit determines that each out-of-focus object has a height relative to the slide surface that is higher than the maximum height or lower than the minimum height of the focusing range of the objective lens. 29. The system of arrangement 27 or 28, wherein the system evaluates the focal point of the object in the first plurality of images by determining, at least in part, whether the object is located at .
[Configuration 29]
Arrangement 23, wherein the image processing unit evaluates the focus of the object in the first plurality of images by at least partially determining the position of each out-of-focus object within the first linear section. 29. The system according to any one of 28 to 28.
[Configuration 30]
30. The system of any one of configurations 23-29, wherein one or both of the camera and the slide move laterally relative to the other during the image acquisition.
[Configuration 31]
the height of the objective lens relative to the sliding surface increases and/or decreases the height of the sliding surface relative to the camera as a function of the linear position of the camera relative to the longitudinal position of each of the linear sections; 31. The system of configuration 30, wherein the system varies by.
[Configuration 32]
The height of the objective lens relative to the sliding surface increases and/or increases the height of the camera relative to the sliding surface as a function of the linear position of the camera relative to the longitudinal position of the first or second linear section. 31. The system of configuration 30, wherein the system changes by: or decreasing.
[Configuration 33]
33. The system of any one of arrangements 23-32, wherein the second plurality of images is acquired from the second straight line segment.
[Configuration 34]
The second plurality of images are acquired from the first straight line portion, and the image processing unit:
evaluating the focus of the object imaged in the second plurality of images; and
the camera configured to acquire a third plurality of images of the second straight line section, the object being imaged in the second plurality of images during the acquisition of the third plurality of images; 33. The system according to any one of arrangements 23-32, wherein the height of the objective relative to the sliding surface is varied based on the estimated focus of the lens.
[Configuration 35]
35. The system of any one of configurations 23-34, wherein the second straight section is directly adjacent to the first straight section.
Claims (18)
前記検体の第１の直線部分の第１の複数の画像を取得することと、
前記第１の複数の画像に撮像された前記検体の前記第１の直線部分内の物体の焦点を評価することであって、焦点外物体の総数が閾値数を超えるかを決定することと、前記第１の直線部分に沿った前記検体の最適な焦点高さを決定することとを含む、評価することと、
前記第１の直線部分に沿った前記検体の前記最適な焦点高さのｚ焦点高さ曲線を計算することと、
前記第１の複数の画像内の焦点外物体の前記総数が前記閾値数を超える場合に、前記検体の前記第１の直線部分を再撮影して第２の複数の画像を取得することであって、前記ｚ焦点高さ曲線に基づいて、前記第２の複数の画像の取得中に前記スライド面に対する前記対物レンズの高さを変化させることと、
前記第１の複数の画像内の焦点外物体の前記総数が前記閾値数を超えない場合に、前記第１の直線部分に直接隣接する前記検体の第２の直線部分の第２の複数の画像を取得することであって、前記ｚ焦点高さ曲線に基づいて、前記第２の複数の画像の取得中に前記スライド面に対する前記対物レンズの高さを変化させることと、を含む方法。 A method of acquiring an image of an object distributed within a specimen affixed to a slide surface using a camera having an objective lens having an optical axis making a non-orthogonal angle to the slide surface , the method comprising: It has an uneven height with respect to the sliding surface,
acquiring a first plurality of images of a first straight section of the specimen;
evaluating the focus of objects within the first linear portion of the specimen captured in the first plurality of images , determining whether a total number of out-of-focus objects exceeds a threshold number; determining an optimal focal height of the specimen along the first straight section;
calculating a z-focal height curve of the optimal focal height of the specimen along the first straight line portion;
reimaging the first linear portion of the specimen to obtain a second plurality of images if the total number of out-of-focus objects in the first plurality of images exceeds the threshold number; changing the height of the objective lens relative to the slide surface during acquisition of the second plurality of images based on the z focal height curve;
a second plurality of images of a second linear section of the specimen immediately adjacent to the first linear section, if the total number of out-of-focus objects in the first plurality of images does not exceed the threshold number; and varying the height of the objective relative to the slide surface during acquisition of the second plurality of images based on the z-focal height curve.
前記第２の複数の画像に撮像された前記物体の焦点を評価することであって、焦点外物体の総数が閾値数を超えるかを決定することと、次の直線部分に沿った前記検体の最適な焦点高さを決定することとを含む、評価することと、
前記第２の直線部分に沿った前記検体の前記最適な焦点高さの第２のｚ焦点高さ曲線を計算することと、
第３の直線部分の第３の複数の画像を取得することとであって、前記第３の直線部分は前記第２の直線部分に直接隣接しており、前記第２のｚ焦点高さ曲線に基づいて、前記第３の複数の画像の取得中に前記スライド面に対する前記対物レンズの高さを変化させることと、を更に含む、請求項１に記載の方法。 the second plurality of images are acquired from the first straight line section, the method comprising:
evaluating the focus of the object imaged in the second plurality of images, determining whether a total number of out-of-focus objects exceeds a threshold number; evaluating, including determining an optimal focal height ;
calculating a second z-focal height curve of the optimal focal height of the specimen along the second straight line portion;
acquiring a third plurality of images of a third straight section, the third straight section being directly adjacent to the second straight section, and wherein the third straight section is directly adjacent to the second straight section; 2. The method of claim 1 , further comprising: varying the height of the objective relative to the slide surface during acquisition of the third plurality of images based on .
（ａ）前記検体の第１の直線部分の第１の複数の画像を取得することと、
（ｂ）前記第１の複数の画像に撮像された前記物体の焦点を評価することであって、焦点外物体の総数が閾値数を超えるかを決定することと、前記第１の直線部分に沿った前記検体の最適な焦点高さを決定することとを含む、評価することと、
（ｃ）前記第１の直線部分に沿った前記検体の前記最適な焦点高さのｚ焦点高さ曲線を計算することと、
（ｄ）前記第１の複数の画像内の焦点外物体の前記総数が前記閾値数を超える場合に、前記検体の前記第１の直線部分を再撮影して第２の複数の画像を取得することであって、前記ｚ焦点高さ曲線に基づいて、前記第２の複数の画像の取得中に前記スライド面に対する前記対物レンズの高さを変化させることと、前記第１の複数の画像内の焦点外物体の前記総数が前記閾値数を超えない場合に、前記第１の直線部分に直接隣接する前記検体の第２の直線部分の第２の複数の画像を取得することであって、前記ｚ焦点高さ曲線に基づいて、前記第２の複数の画像の取得中に前記スライド面に対する前記対物レンズの高さを変化させることと、
（ｅ）（ａ）～（ｄ）を検体のほぼ全体の画像が取得されるまで繰り返すこととを含む、方法。 A method of acquiring an image of an object distributed within a specimen affixed to a slide surface using a camera having an objective lens having an optical axis making a non-orthogonal angle to the slide surface , the method comprising: having a non-uniform height with respect to the slide surface, the method includes:
(a) acquiring a first plurality of images of a first straight section of the specimen;
(b) evaluating the focus of the object imaged in the first plurality of images , determining whether a total number of out-of-focus objects exceeds a threshold number; and determining an optimal focal height of the specimen along the
(c) calculating a z-focal height curve of the optimal focal height of the specimen along the first straight line portion;
(d) reimaging the first linear portion of the specimen to obtain a second plurality of images if the total number of out-of-focus objects in the first plurality of images exceeds the threshold number; changing the height of the objective relative to the slide surface during acquisition of the second plurality of images based on the z focal height curve; acquiring a second plurality of images of a second linear section of the specimen immediately adjacent to the first linear section if the total number of out-of-focus objects of the object does not exceed the threshold number; varying the height of the objective relative to the slide surface during acquisition of the second plurality of images based on the z focal height curve;
( e ) repeating steps (a) to ( d ) until an image of substantially the entire specimen is acquired.
光軸を有する対物レンズを有するカメラであって、前記光軸が前記スライド面と非直交角をなすように位置決めされた前記カメラと、
前記カメラと動作可能に接続された画像処理部とを備え、
請求項１から１２のいずれか一項に記載の方法を実行するように構成されたシステム。 A system for acquiring an image of an object distributed within a specimen affixed to a slide surface , the specimen having a non-uniform height with respect to the slide surface, the system comprising:
a camera having an objective lens having an optical axis, the camera being positioned such that the optical axis makes a non-orthogonal angle to the slide surface ;
an image processing unit operably connected to the camera;
A system configured to carry out a method according to any one of claims 1 to 12.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019046304A1 (en) | 2017-08-28 | 2019-03-07 | Matthias Wagner | Microfluidic laser-activated intracellular delivery systems and methods |
| DE102021101439A1 (en) * | 2021-01-22 | 2022-07-28 | Carl Zeiss Microscopy Gmbh | MICROSCOPY SYSTEM AND METHOD OF ROTATIONAL CHECKING OF A MICROSCOPE CAMERA |
| EP4304780A1 (en) * | 2021-03-07 | 2024-01-17 | Cellino Biotech, Inc. | Platforms and systems for automated cell culture |
| US11931737B2 (en) | 2021-09-02 | 2024-03-19 | Cellino Biotech, Inc. | Platforms and systems for automated cell culture |
| EP4198892A1 (en) * | 2021-12-14 | 2023-06-21 | Leica Microsystems CMS GmbH | Method for determining boundaries of a z-stack of images of an object, corresponding optical instrument and computer program therefore |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5269918A (en) | 1990-07-09 | 1993-12-14 | Cytyc Corporation | Clinical cartridge apparatus |
| US5240606A (en) | 1990-07-09 | 1993-08-31 | Cytyc Corporation | Apparatus for preparing cells for examination |
| US5282978A (en) | 1990-07-09 | 1994-02-01 | Cytyc Corporation | Specimen processor method and apparatus |
| US5143627A (en) | 1990-07-09 | 1992-09-01 | Cytyc Corporation | Method and apparatus for preparing cells for examination |
| CA2229175A1 (en) * | 1998-02-06 | 1999-08-06 | Morphometrix Technologies Inc. | Automatic focus system |
| US6562299B1 (en) | 1998-09-18 | 2003-05-13 | Cytyc Corporation | Method and apparatus for preparing cytological specimens |
| US6572824B1 (en) | 1998-09-18 | 2003-06-03 | Cytyc Corporation | Method and apparatus for preparing cytological specimens |
| US6665060B1 (en) | 1999-10-29 | 2003-12-16 | Cytyc Corporation | Cytological imaging system and method |
| US7006674B1 (en) | 1999-10-29 | 2006-02-28 | Cytyc Corporation | Apparatus and methods for verifying the location of areas of interest within a sample in an imaging system |
| US7369304B2 (en) | 1999-10-29 | 2008-05-06 | Cytyc Corporation | Cytological autofocusing imaging systems and methods |
| US7668362B2 (en) * | 2000-05-03 | 2010-02-23 | Aperio Technologies, Inc. | System and method for assessing virtual slide image quality |
| US7771662B2 (en) | 2001-10-19 | 2010-08-10 | Hologic, Inc | Vial system and method for processing liquid-based specimens |
| KR101106358B1 (en) | 2003-06-12 | 2012-01-18 | 사이틱 코포레이션 | System for organizing multiple objects of interest in field of interest |
| US7556777B2 (en) | 2005-03-08 | 2009-07-07 | Cytyc Corporation | Specimen vial cap handler and slide labeler |
| US7587078B2 (en) | 2005-05-02 | 2009-09-08 | Cytyc Corporation | Automated image analysis |
| US8648959B2 (en) * | 2010-11-11 | 2014-02-11 | DigitalOptics Corporation Europe Limited | Rapid auto-focus using classifier chains, MEMS and/or multiple object focusing |
| JP6147006B2 (en) * | 2013-01-09 | 2017-06-14 | オリンパス株式会社 | Imaging apparatus, microscope system, and imaging method |
| EP3037861A1 (en) * | 2014-12-23 | 2016-06-29 | Commissariat à l'Énergie Atomique et aux Énergies Alternatives | Imaging method, and system, for obtaining an super-resolution image of an object |
| CN108369337B (en) * | 2015-12-09 | 2021-03-30 | 文塔纳医疗系统公司 | Image scanning apparatus and method of operating the same |
| JP2019537067A (en) * | 2016-12-01 | 2019-12-19 | バークレー ライツ，インコーポレイテッド | Apparatus, system, and method for imaging a minute object |
| JP6899963B2 (en) * | 2017-09-29 | 2021-07-07 | ライカ バイオシステムズ イメージング インコーポレイテッドＬｅｉｃａ Ｂｉｏｓｙｓｔｅｍｓ Ｉｍａｇｉｎｇ， Ｉｎｃ． | Real-time autofocus scanning |
| WO2020091965A2 (en) | 2018-11-02 | 2020-05-07 | Hologic, Inc. | Digital imaging system and method |
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