JPWO2021100709A1 - - Google Patents

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Publication number
JPWO2021100709A1
JPWO2021100709A1 JP2021558396A JP2021558396A JPWO2021100709A1 JP WO2021100709 A1 JPWO2021100709 A1 JP WO2021100709A1 JP 2021558396 A JP2021558396 A JP 2021558396A JP 2021558396 A JP2021558396 A JP 2021558396A JP WO2021100709 A1 JPWO2021100709 A1 JP WO2021100709A1
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JP
Japan
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Application number
JP2021558396A
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Publication of JPWO2021100709A1 publication Critical patent/JPWO2021100709A1/ja
Priority to JP2022130662A priority Critical patent/JP2022163218A/ja
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Vascular Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021558396A 2019-11-19 2020-11-17 Pending JPWO2021100709A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022130662A JP2022163218A (ja) 2019-11-19 2022-08-18 細胞構造体及びその製造方法並びに被験物質の肝毒性の評価方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2019208933 2019-11-19
PCT/JP2020/042824 WO2021100709A1 (ja) 2019-11-19 2020-11-17 細胞構造体及びその製造方法並びに被験物質の肝毒性の評価方法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022130662A Division JP2022163218A (ja) 2019-11-19 2022-08-18 細胞構造体及びその製造方法並びに被験物質の肝毒性の評価方法

Publications (1)

Publication Number Publication Date
JPWO2021100709A1 true JPWO2021100709A1 (ja) 2021-05-27

Family

ID=75980575

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021558396A Pending JPWO2021100709A1 (ja) 2019-11-19 2020-11-17
JP2022130662A Pending JP2022163218A (ja) 2019-11-19 2022-08-18 細胞構造体及びその製造方法並びに被験物質の肝毒性の評価方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2022130662A Pending JP2022163218A (ja) 2019-11-19 2022-08-18 細胞構造体及びその製造方法並びに被験物質の肝毒性の評価方法

Country Status (5)

Country Link
US (1) US20220411750A1 (ja)
EP (1) EP4063486A4 (ja)
JP (2) JPWO2021100709A1 (ja)
CN (1) CN114729307A (ja)
WO (1) WO2021100709A1 (ja)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115003796A (zh) * 2020-01-20 2022-09-02 凸版印刷株式会社 立体性细胞结构体的制造方法
EP4253528A1 (en) * 2020-11-26 2023-10-04 Toppan Inc. Three-dimensional cell tissue production method and three-dimensional cell tissue
JPWO2023017800A1 (ja) * 2021-08-11 2023-02-16
WO2023120573A1 (ja) * 2021-12-22 2023-06-29 凸版印刷株式会社 立体的細胞組織の培養方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008044351A1 (fr) * 2006-10-12 2008-04-17 National University Corporation Okayama University Nouvelle protéine transporteuse chez les mammifères et son utilisation
WO2015068253A1 (ja) * 2013-11-08 2015-05-14 株式会社島津製作所 肝組織培養用デバイス、肝組織培養用システム、肝組織培の培養方法及び肝機能の評価方法
WO2016148216A1 (ja) * 2015-03-18 2016-09-22 国立大学法人 東京大学 肝細胞及び肝非実質細胞、並びにそれらの調製方法
JP2018530341A (ja) * 2015-10-15 2018-10-18 ウェイク・フォレスト・ユニヴァーシティ・ヘルス・サイエンシズ インビトロで肝臓構築物を産生する方法およびその使用
JP2019033732A (ja) * 2017-08-21 2019-03-07 凸版印刷株式会社 立体的肝臓組織構造体及びその製造方法
WO2019208832A1 (ja) * 2018-04-27 2019-10-31 凸版印刷株式会社 細胞外マトリックス含有組成物及びその製造方法、並びに三次元組織体、三次元組織体形成剤
WO2021066196A1 (ja) * 2019-10-04 2021-04-08 国立研究開発法人産業技術総合研究所 ３次元肝組織モデル

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5850419B2 (ja) 2010-11-11 2016-02-03 国立大学法人大阪大学 細胞の三次元構造体、及び、これを製造する方法
EP4349972A3 (en) 2016-02-22 2024-05-29 Osaka University Method for producing three-dimensional cell tissue
EP3578638A4 (en) 2017-01-31 2020-10-14 Toppan Printing Co., Ltd. THREE-DIMENSIONAL TISSUE BODY, METHOD OF MANUFACTURING THEREOF AND MOLDING AGENT FOR THREE-DIMENSIONAL TISSUE BODY

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008044351A1 (fr) * 2006-10-12 2008-04-17 National University Corporation Okayama University Nouvelle protéine transporteuse chez les mammifères et son utilisation
WO2015068253A1 (ja) * 2013-11-08 2015-05-14 株式会社島津製作所 肝組織培養用デバイス、肝組織培養用システム、肝組織培の培養方法及び肝機能の評価方法
WO2016148216A1 (ja) * 2015-03-18 2016-09-22 国立大学法人 東京大学 肝細胞及び肝非実質細胞、並びにそれらの調製方法
JP2018530341A (ja) * 2015-10-15 2018-10-18 ウェイク・フォレスト・ユニヴァーシティ・ヘルス・サイエンシズ インビトロで肝臓構築物を産生する方法およびその使用
JP2019033732A (ja) * 2017-08-21 2019-03-07 凸版印刷株式会社 立体的肝臓組織構造体及びその製造方法
WO2019208832A1 (ja) * 2018-04-27 2019-10-31 凸版印刷株式会社 細胞外マトリックス含有組成物及びその製造方法、並びに三次元組織体、三次元組織体形成剤
WO2021066196A1 (ja) * 2019-10-04 2021-04-08 国立研究開発法人産業技術総合研究所 ３次元肝組織モデル

Non-Patent Citations (9)

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Title
GUIDELINES FOR USE, PRODUCT: CORNING MATRIGEL BASEMENT MEMBRANE MATRIX GROWTH FACTOR REDUCED, 10 ML, vol. SPC-354230 Rev 8.0, pp.1-4, JPN6022024716, 2013, ISSN: 0005020720 *
MATSUSAKI M. ET AL.: "Construction of Thick 3D-Tissues with Cell Density Gradient by Coating Collagen Nanofiber Matrix on", 高分子学会予稿集, vol. Vol.64, No.1, 1Pf096, JPN6021001183, 2015, ISSN: 0005020715 *
NATURE PROTOCOLS, vol. Vol.9, No.2, pp.396-409, JPN7022002784, 2014, ISSN: 0005020719 *
PLOS ONE, JPN6022024717, 2015, ISSN: 0005020721 *
杉山雄一他 編, 総合製剤学, JPN6021001186, 2000, pages 113 - 114, ISSN: 0005020717 *
松崎典弥 他: "コラーゲンマイクロファイバーを用いた沈殿培養による高密度ECMを有する3D-結合組織の構築", 高分子学会予稿集, vol. Vol.66, No.2, 3N02, JPN6019030164, 2017, ISSN: 0005020714 *
林芳弘 他: "膵星細胞と肝星細胞の比較−特に形態学および発生学的観点から−", 肝胆膵, vol. Vol.55, No.6, pp.1187-1197, JPN6021001188, 2007, ISSN: 0005020718 *
米田美咲 他: "コラーゲンマイクロファイバーによる高濃度細胞外マトリックスと毛細血管網を有する三次元間質組織体の構築", 高分子学会予稿集, vol. Vol.66, No.2, 2Q10, JPN6019030161, 2017, ISSN: 0005020713 *
素輪善弘 他: "移植に適した血管網を付加したIn vitro脂肪組織構築の試み", 第２８回日本形成外科学会基礎学術集会 プログラム・抄録集, JPN6021001189, 14 November 2019 (2019-11-14), pages 113 - 2, ISSN: 0005020716 *

Also Published As

Publication number Publication date
CN114729307A (zh) 2022-07-08
US20220411750A1 (en) 2022-12-29
JP2022163218A (ja) 2022-10-25
WO2021100709A1 (ja) 2021-05-27
EP4063486A4 (en) 2024-01-10
EP4063486A1 (en) 2022-09-28

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