JPWO2021076917A5 - - Google Patents
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- JPWO2021076917A5 JPWO2021076917A5 JP2022523282A JP2022523282A JPWO2021076917A5 JP WO2021076917 A5 JPWO2021076917 A5 JP WO2021076917A5 JP 2022523282 A JP2022523282 A JP 2022523282A JP 2022523282 A JP2022523282 A JP 2022523282A JP WO2021076917 A5 JPWO2021076917 A5 JP WO2021076917A5
- Authority
- JP
- Japan
- Prior art keywords
- fermentation product
- beta
- enzyme
- lyase activity
- 4mmp
- Prior art date
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Claims (26)
(b)ベータ-リアーゼ活性をもつ酵素が、配列番号4もしくは5のいずれか1つで表されるとおりの配列と少なくとも90%の配列同一性をもつ配列を有する、請求項1に記載の修飾された細胞。 (a) the enzyme with beta-lyase activity has a sequence having at least 90% sequence identity with the sequence as represented by SEQ ID NO: 2 ; or
(b) the modification of claim 1, wherein the enzyme with beta-lyase activity has a sequence with at least 90% sequence identity to the sequence as represented by either one of SEQ ID NOs: 4 or 5. cells.
配列番号1の位置H463に対応する位置での置換変異が、フェニルアラニン、アルギニン、グルタミン酸、トレオニン、グリシン、イソロイシン、またはバリンである、請求項1~5のいずれか一項に記載の修飾された細胞。 The enzyme with beta-lyase activity contains a substitution mutation at the position corresponding to position H463 of SEQ ID NO: 1, and optionally
Modified cell according to any one of claims 1 to 5 , wherein the substitution mutation at the position corresponding to position H463 of SEQ ID NO: 1 is phenylalanine, arginine, glutamic acid, threonine, glycine, isoleucine, or valine. .
発酵性糖が、グルコース、フルクトース、スクロース、マルトース、および/またはマルトトリオースである、請求項10に記載の方法。 at least one fermentable sugar is provided in at least one sugar source ; optionally
11. The method according to claim 10 , wherein the fermentable sugar is glucose, fructose, sucrose, maltose, and/or maltotriose .
少なくとも1つの前駆体が、システインに抱合された3-メルカプトヘキサン-1-オール(Cys 3-MH)、システインに抱合された4-メチル-4-メルカプトペンタン-2-オン(Cys 4MMP)、グルタチオンに抱合された3-メルカプトヘキサン-1-オール(Glut-3-MH)、および/またはグルタチオンに抱合された4-メチル-4-メルカプトペンタン2-オン(Glut 4MMP)を含む、請求項10または11に記載の方法。 at least one sugar source comprises at least one precursor, optionally
The at least one precursor is 3-mercaptohexan-1-ol conjugated to cysteine (Cys 3-MH), 4-methyl-4-mercaptopentan-2-one conjugated to cysteine (Cys 4MMP), glutathione 10. 3-mercaptohexan-1-ol conjugated to (Glut-3-MH) and/or 4-methyl-4-mercaptopentan-2-one conjugated to glutathione (Glut 4MMP). or the method described in 11 .
少なくとも1つの揮発性チオールが、3-メルカプトヘキサン-1-オール(3MH)、3-メルカプト酢酸ヘキシル(3MHA)、4-メチル-4-メルカプトペンタ-2-オン(4MMP)、またはそれらの組み合わせを含む、請求項10~13のいずれか一項に記載の方法。 said fermentation product has an increased level of at least one volatility as compared to a fermentation product produced by an equivalent cell that does not express the heterologous gene or an equivalent cell that expresses a wild-type enzyme having beta-lyase activity. Contains thiol ; optionally
The at least one volatile thiol is 3-mercaptohexan-1-ol (3MH), hexyl 3-mercaptoacetate (3MHA), 4-methyl-4-mercaptopent-2-one (4MMP), or a combination thereof. 14. A method according to any one of claims 10 to 13 , comprising :
少なくとも1つの望ましくない産物が、インドールである、請求項10~15のいずれか一項に記載の方法。 said fermentation product has a reduced level of at least one undesirable product compared to a fermentation product produced by an equivalent cell that does not express the heterologous gene or an equivalent cell that expresses a wild-type enzyme having beta-lyase activity. including products ; optionally
16. A method according to any one of claims 10 to 15 , wherein the at least one undesired product is indole .
方法が、培地を産生することをさらに含み、ここで培地を産生することが、以下:
(a)複数の穀物を水と接触させること;ならびに
(b)水および穀物を煮沸するかまたは浸すことで、麦芽汁を産生すること
を含む、請求項10~20のいずれか一項に記載の方法。 the sugar source is wort, and the method further comprises producing a culture medium, wherein producing the culture medium comprises:
(a) contacting the grains with water; and
21. A method according to any one of claims 10 to 20 , comprising: (b) boiling or soaking water and grain to produce wort.
発酵産物が、500μg/L未満のインドールを含む、請求項25に記載の発酵産物。 The fermentation product contains at least 200 ng/L of 3-mercaptohexan-1-ol (3MH), hexyl 3-mercaptoacetate (3MHA), and/or 4-methyl-4-mercaptopent-2-one (4MMP). including and/or
26. The fermentation product of claim 25 , wherein the fermentation product contains less than 500 μg/L indole .
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962916529P | 2019-10-17 | 2019-10-17 | |
US62/916,529 | 2019-10-17 | ||
US202063086363P | 2020-10-01 | 2020-10-01 | |
US63/086,363 | 2020-10-01 | ||
PCT/US2020/056022 WO2021076917A1 (en) | 2019-10-17 | 2020-10-16 | Genetically engineered yeast cells and methods of use thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2022553039A JP2022553039A (en) | 2022-12-21 |
JPWO2021076917A5 true JPWO2021076917A5 (en) | 2023-10-24 |
Family
ID=75538349
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2022523282A Pending JP2022553039A (en) | 2019-10-17 | 2020-10-16 | Genetically modified yeast cells and methods of use thereof |
Country Status (9)
Country | Link |
---|---|
US (1) | US20240150687A1 (en) |
EP (1) | EP4045670A4 (en) |
JP (1) | JP2022553039A (en) |
CN (1) | CN114829618A (en) |
AU (1) | AU2020366440A1 (en) |
BR (1) | BR112022007162A2 (en) |
CA (1) | CA3158152A1 (en) |
MX (1) | MX2022004592A (en) |
WO (1) | WO2021076917A1 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4291627A1 (en) * | 2021-02-10 | 2023-12-20 | Omega Yeast Labs, LLC | Materials and methods for brewing beer |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007011845A2 (en) * | 2005-07-18 | 2007-01-25 | Basf Ag | Use of a bacillus meti gene to improve methionine production in microorganisms |
WO2007095682A1 (en) * | 2006-02-24 | 2007-08-30 | The Australian Wine Research Institute | Methods and micro-organisms for modulating the conversion of non-volatile sulfur compounds to volatile thiol compounds |
US11685925B2 (en) * | 2015-10-30 | 2023-06-27 | Synlogic Operating Company, Inc. | Bacteria engineered to treat diseases that benefit from reduced gut inflammation and/or tightened gut mucosal barrier |
-
2020
- 2020-10-16 CN CN202080087745.XA patent/CN114829618A/en active Pending
- 2020-10-16 MX MX2022004592A patent/MX2022004592A/en unknown
- 2020-10-16 JP JP2022523282A patent/JP2022553039A/en active Pending
- 2020-10-16 AU AU2020366440A patent/AU2020366440A1/en active Pending
- 2020-10-16 CA CA3158152A patent/CA3158152A1/en active Pending
- 2020-10-16 US US17/768,257 patent/US20240150687A1/en active Pending
- 2020-10-16 EP EP20877078.4A patent/EP4045670A4/en active Pending
- 2020-10-16 WO PCT/US2020/056022 patent/WO2021076917A1/en unknown
- 2020-10-16 BR BR112022007162A patent/BR112022007162A2/en unknown
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