JPWO2021072408A5

JPWO2021072408A5 -

Info

Publication number: JPWO2021072408A5
Application number: JP2022521445A
Authority: JP
Publication date: 2023-10-16

Claims

a light source that outputs a light beam containing an ultraviolet wavelength for illuminating the biological sample;
a receiving space that receives the biological sample illuminated by the light beam output from the light source;
an ultraviolet imaging objective configured to collect light that interacts with the biological sample and relay the collected light to an imaging device;
a UV-sensitive imager for capturing an image of the relayed light from the UV imaging objective;
configured to capture one or more images of the biological sample at one or more of the ultraviolet wavelengths , and
A deep ultraviolet imaging system that analyzes one or more images of the biological sample to classify or characterize one or more cells within the biological sample.

further comprising one or more bandpass filters disposed downstream of the light source,
the light source comprises a broadband light source configured to output the light beam further including non -ultraviolet wavelengths;
The deep ultraviolet light of claim 1, wherein one or more of the bandpass filters are configured to filter the light beam output from the light source to remove non-ultraviolet wavelengths and relay ultraviolet wavelengths. Imaging system.

3. The deep ultraviolet imaging system of claim 2, wherein the system comprises two or more bandpass filters.

4. The deep ultraviolet imaging system of claim 3, wherein the two or more bandpass filters are supported by a filter stage configured to switch between the two or more bandpass filters.

One or more of the bandpass filters are each configured to pass a narrow band of ultraviolet wavelength light centered around a wavelength in the range of 200 to 450 nm, the narrow band having a bandwidth of 50 nm or less. 3. The deep ultraviolet imaging system of claim 2, comprising:

a short-pass dichroic mirror for filtering non-ultraviolet wavelengths from the light beam output from the light source, the short-pass dichroic mirror being disposed between the light source and one or more of the bandpass filters; The deep ultraviolet imaging system of claim 2, further comprising a mirror.

a parabolic mirror for removing chromatic aberration and converging the light rays output from the light source, the parabolic mirror being disposed between the light source and one or more of the bandpass filters; The deep ultraviolet imaging system of claim 2, further comprising:

7. The light source is one or more LEDs, each LED configured to output a light beam consisting of a narrow band of ultraviolet wavelengths, the narrow band comprising a bandwidth of 50 nm or less. The deep ultraviolet imaging system according to any one of .

The light source is two or more LEDs, each LED configured to output a light beam consisting of a narrow band of ultraviolet wavelengths, each LED outputting an ultraviolet wavelength that is different from the ultraviolet wavelengths of the other LEDs. 9. The deep ultraviolet imaging system of claim 8, wherein the deep ultraviolet imaging system is configured to.

9. The deep ultraviolet imaging system of claim 8, wherein the light source comprises one or more LEDs each emitting light in a narrow band of ultraviolet wavelengths centered around wavelengths in the range of 200-400 nm.

11. Any one of claims 1 to 10, wherein the ultraviolet imaging objective is adapted to collect light that interacts with the biological sample, which may include absorption and scattering in transmission and back reflection. A deep ultraviolet imaging system as described in .

Analyzing one or more images of the biological sample to classify or characterize one or more cells within the biological sample comprises:
using machine learning algorithms;
converting one or more of the images of the biological sample into one or more corresponding color images;
identifying and/or phenotyping unique types of cells within the biological sample;
phenotyping blood, bone marrow, and/or tissue within the biological sample;
12. A deep ultraviolet imaging system according to any one of claims 1 to 11, comprising one or more of:

Analyzing one or more images of the biological sample to classify or characterize one or more cells within the biological sample comprises:
converting one or more of the images of the biological sample into one or more corresponding color images;
one or more of the images of the biological sample with a blank image taken at one or more of the ultraviolet wavelengths before converting the one or more of the images into one or more corresponding color images; to normalize,
Prior to converting one or more of the images of the biological sample into one or more corresponding color images, one or more of the images are subjected to weighting factors selected based on one or more of the ultraviolet wavelengths and scaling using a gamma factor;
A deep ultraviolet imaging system according to any one of claims 1 to 11, comprising one or more of:

Converting one or more of the images of the biological sample into one or more corresponding color images comprises:
assigning one or more of the images to channels in an RGB color space based on one or more of the ultraviolet wavelengths;
performing the conversion so that one or more colors of the corresponding color image distinguish molecules or structures in the image;
14. A deep ultraviolet imaging system according to claim 12 or 13, comprising one or more of:

Capturing one or more of the images of the biological sample at one or more of the ultraviolet wavelengths includes narrow band ultraviolet wavelengths having a bandwidth of 50 nm or less and centered around wavelengths in the range of 255-260 nm. 15. A deep ultraviolet imaging system according to any one of claims 1 to 14, comprising capturing one or more of the images from a single ultraviolet channel obtained from.

A method for imaging a biological sample, the method comprising:
A method comprising imaging the biological sample with a deep ultraviolet imaging system according to claim 1.

irradiating the biological sample with a light beam from the light source;
capturing one or more images of the biological sample at one or more of the ultraviolet wavelengths;
and analyzing one or more of the images taken at one or more of the ultraviolet wavelengths to classify or characterize one or more cells within the biological sample. Methods for imaging the described biological samples.

18. The method of imaging a biological sample according to claim 16 or 17, wherein one or more of the ultraviolet wavelengths are narrow band ultraviolet wavelengths having a bandwidth of 50 nm or less and centered at wavelengths in the range of 255-260 nm. .

A method of processing an ultraviolet image of a biological sample, the method comprising:
receiving a plurality of multispectral ultraviolet images of a biological sample, the ultraviolet images including images taken at three or more ultraviolet wavelengths;
normalizing each UV image with a blank image taken at a wavelength corresponding to the given UV image;
scaling each ultraviolet image with a weighting factor and a gamma factor, the weighting factor and gamma factor for each given ultraviolet image being selected based on the wavelength of the given ultraviolet image; to do and
Assigning each scaled ultraviolet image to a channel of an RGB color space based on the wavelength of the scaled ultraviolet image includes assigning the ultraviolet image of a first wavelength to the red channel and assigning the ultraviolet image of a second wavelength to a channel of the RGB color space. and assigning a third wavelength ultraviolet image to the blue channel.