CN212514276U - Wide-spectrum fluorescence multi-channel real-time imaging system - Google Patents
Wide-spectrum fluorescence multi-channel real-time imaging system Download PDFInfo
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- CN212514276U CN212514276U CN202020920903.2U CN202020920903U CN212514276U CN 212514276 U CN212514276 U CN 212514276U CN 202020920903 U CN202020920903 U CN 202020920903U CN 212514276 U CN212514276 U CN 212514276U
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Abstract
The utility model discloses a wide spectrum fluorescence multichannel real-time imaging system, it includes: the excitation light source is at least used for providing first excitation light and second excitation light with different wavelengths to irradiate a sample to be detected, so that the sample to be detected is excited to emit mixed fluorescence containing multiple wavelengths of fluorescence; the first light splitting structure is used for separating the mixed fluorescence into first fluorescence and second fluorescence with different wave bands; the second light splitting structure comprises a first light splitting module and a second light splitting module, the first light splitting module is used for splitting the received first fluorescence into a plurality of first pulse lights with different wave bands, and the second light splitting module is used for splitting the received second fluorescence into a plurality of second pulse lights with different wave bands; the excitation light source, the first detector and the second detector are respectively connected with the control unit. The utility model discloses a wide spectrum fluorescence multichannel real-time imaging system can realize functions such as multichannel real-time imaging, multichannel chronogenesis formation of image.
Description
Technical Field
The utility model relates to a fluorescence imaging system, in particular to wide spectrum fluorescence multichannel real-time imaging system belongs to fluorescence imaging technology field.
Background
At present, the fluorescence imaging technology is widely applied to the research of life science, and particularly, the fluorescence imaging technology with a large visual field has wide application prospect in the aspect of medical diagnosis and treatment of living organisms. The technology is to utilize a detector to realize imaging on a biological or biological tissue specifically marked by a fluorescent probe. A typical fluorescence imaging system includes an excitation light source, filters, lenses, a fluorescent sample, and a detector. Excitation light from the light source uniformly irradiates on the sample, fluorescence generated by the sample being excited is collected by the objective lens and imaged on the detector, so that the fluorescence imaging has the capability of providing specific position information. The working spectral range of fluorescence imaging in the market at present is mainly located in visible light (400-. These three bands have respective irreplaceable effects depending on the specificity of the fluorescent probe. At present, a detector can realize fluorescence imaging of 400-1700nm waveband, but the fluorescence imaging of different wavebands cannot be realized simultaneously, and the problem of low quantum efficiency of partial waveband (less than 50%) exists. The multi-detector is utilized to respectively image the fluorescence with different wave bands, and the problem that multi-band co-location imaging cannot be realized exists. In conclusion, a device capable of realizing 400-1700nm wide spectrum imaging and high quantum efficiency (> 75%) multiband co-localization real-time imaging does not exist.
Multiple sources of information can be obtained by marking a plurality of different fluorescent probes, for example, the distribution of transplanted stem cells in different tissues and organs and the death and survival states of the transplanted stem cells can be clearly indicated, which cannot be realized by single-probe imaging. Especially, simultaneous labeling and tracing imaging of multiple probes are of great significance. At present, the function of distinguishing different colors by utilizing a color CCD (charge coupled device) in a visible light (400-650nm) wave band can realize the simultaneous imaging of various probes of visible light. However, color CCDs have a poor signal-to-noise ratio compared to monochrome CCDs, and there is signal crosstalk. In the near infrared band (650-1700nm), only a monochromatic detector is provided, and the detector in the band can only identify the fluorescence intensity of the probe and cannot distinguish the wavelength of the fluorescence, so that the simultaneous tracing of multiple probes in the band cannot be realized.
SUMMERY OF THE UTILITY MODEL
The main object of the utility model is to provide a wide spectrum fluorescence multichannel real-time imaging system to overcome not enough among the prior art.
For realizing the purpose of the utility model, the utility model discloses a technical scheme include:
the embodiment of the utility model provides a wide spectrum fluorescence multichannel real-time imaging system, it includes:
the excitation light source is at least used for providing first excitation light and second excitation light with different wavelengths to irradiate a sample to be detected, so that the sample to be detected is excited to emit mixed fluorescence containing multiple wavelengths of fluorescence;
the first light splitting structure is at least used for separating the mixed fluorescence into first fluorescence and second fluorescence with different wave bands and enabling the first fluorescence and the second fluorescence to be output to the second light splitting structure;
the second light splitting structure comprises a first light splitting module and a second light splitting module, wherein the first light splitting module is used for splitting the received first fluorescence into a plurality of first pulse lights with different wave bands and inputting the plurality of first pulse lights into the first detector, and the second light splitting module is used for splitting the received second fluorescence into a plurality of second pulse lights with different wave bands and inputting the plurality of second pulse lights into the second detector;
the excitation light source, the first detector and the second detector are respectively connected with the control unit.
Further, the first light splitting structure includes a dichroic mirror.
Further, the dichroic mirror is a plurality of dichroic mirrors that are switchable.
Further, the first light splitting module comprises a plurality of first optical filters, the plurality of first optical filters are respectively matched with the plurality of first pulsed lights with different wave bands, and the first fluorescent light respectively passes through the plurality of first optical filters to obtain the plurality of first pulsed lights with different wave bands.
Furthermore, the plurality of first optical filters are connected with a first optical filter switching component, the first optical filter switching component is connected with the control unit, and the first optical filter switching component can switch the positions of the plurality of first optical filters according to a preset sequence, so that the received first fluorescent light respectively passes through the plurality of first optical filters in sequence, and then the first pulse light with a plurality of different wave bands is obtained.
Further, the second light splitting module includes a plurality of second filters, the plurality of second filters are respectively matched with the plurality of second pulsed lights with different wave bands, and the second fluorescent light respectively passes through the plurality of second filters to obtain the plurality of second pulsed lights with different wave bands.
Furthermore, the plurality of second optical filters are connected with a second optical filter switching component, the second optical filter switching component is connected with the control unit, and the second optical filter switching component can switch the positions of the plurality of second optical filters according to a preset sequence, so that the received second fluorescent light respectively passes through the plurality of second optical filters in sequence, and then second pulsed light with a plurality of different wave bands is obtained.
Further, the first pulse light and the second pulse light are respectively input into the first detector and the second detector through the first imaging objective lens and the second imaging objective lens.
Further, the first detector and the second detector are arranged in an optical coaxial manner, the first detector comprises an indium gallium arsenide camera, and the second detector comprises a silicon-based CCD.
Further, the excitation light source includes a first excitation light source and a second excitation light source for providing a first excitation light and a second excitation light, respectively, where the first excitation light source includes a visible light source, and the second excitation light source includes a near-infrared laser.
Further, the wavelength of the mixed fluorescence is 400-1700 nm.
Further, the control unit includes an I/O device having a clock function.
Further, the I/O device is also connected with a computer.
Compared with the prior art, the utility model has the advantages that:
the embodiment of the utility model provides a wide spectrum fluorescence multichannel real-time imaging system constructs multilayer beam splitting structure with multichannel dichroic mirror and a plurality of optical filters that can be switched by programs, and the mixed fluorescence signal is separated according to wave band in real time; finally, imaging is carried out on the detector by an imaging objective lens; the detector pack comprises a silicon-based CCD and an InGaAs camera which are designed to be optical coaxial, so that co-location imaging can be realized, and the high quantum efficiency of the 400-cottony 1700nm wide band is ensured;
the embodiment of the utility model provides a pair of real-time imaging system of wide spectrum fluorescence multichannel to the IO device that has the external clock function is comprehensive control center, through corresponding control program, realizes the work is accomplished simultaneously or with certain time sequence to excitation light source, the optical filter that multiunit programming switched of the different wavelength of control multiunit, multiunit detector, realizes functions such as multichannel real-time imaging, multichannel chronogenesis formation of image, thereby realizes multiband, many probes spike simultaneously.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram of a broad spectrum fluorescence multi-channel real-time imaging system in an exemplary embodiment of the present invention;
description of reference numerals: the device comprises a visible light source-1, a near-infrared laser-2, a sample objective table-3, a dichroic mirror-4, a first optical filter 11-, a first optical filter switching component-6, a second optical filter-12, a second optical filter switching component-5, a visible light imaging objective lens-13, a near-infrared imaging objective lens-14, an indium gallium arsenic camera-7, a silicon-based CCD-8, an I/O device-9 with an external clock function and a computer 10.
Detailed Description
In view of the deficiencies in the prior art, the inventor of the present invention has made extensive studies and practices to provide the technical solution of the present invention. The technical solution, its implementation and principles, etc. will be further explained as follows.
The embodiment of the utility model provides a wide spectrum fluorescence multichannel real-time imaging system, it includes:
the excitation light source is at least used for providing first excitation light and second excitation light with different wavelengths to irradiate a sample to be detected, so that the sample to be detected is excited to emit mixed fluorescence containing multiple wavelengths of fluorescence;
the first light splitting structure is at least used for separating the mixed fluorescence into first fluorescence and second fluorescence with different wave bands and enabling the first fluorescence and the second fluorescence to be output to the second light splitting structure;
the second light splitting structure comprises a first light splitting module and a second light splitting module, wherein the first light splitting module is used for splitting the received first fluorescence into a plurality of first pulse lights with different wave bands and inputting the plurality of first pulse lights into the first detector, and the second light splitting module is used for splitting the received second fluorescence into a plurality of second pulse lights with different wave bands and inputting the plurality of second pulse lights into the second detector;
the excitation light source, the first detector and the second detector are respectively connected with the control unit.
The embodiment of the utility model provides a wide spectrum fluorescence multichannel real-time imaging system, this system be one kind can realize the wide spectrum (400) and the near infrared band probe of multiple visible light probe and multiple near infrared band probe tracer simultaneously and mend the multichannel real-time imaging equipment to can be applied to the wide spectrum fluorescence multichannel real-time imaging of live body formation of image wavelength range 400 and the 1700 nm; the system takes a plurality of visible lights and a plurality of near infrared lights as excitation light sources, and can realize that a plurality of light sources simultaneously excite a sample; exciting a mixed fluorescence signal containing fluorescence of multiple wavelengths by using a sample carrying multiple probes; a multi-layer optical signal separation structure is constructed by taking the dichroic mirror and the plurality of optical filters which can be switched in a programmed manner as core elements, and mixed fluorescent signals are separated in real time according to wave bands; finally, imaging is carried out on the detector by an imaging objective lens; the detector pack comprises a silicon-based CCD and an InGaAs camera which are designed to be optical coaxial, so that co-location imaging can be realized, and the high quantum efficiency of the 400-51 nm wide waveband 1700nm is ensured.
In addition, the embodiment of the utility model provides a wide spectrum fluorescence multichannel real-time imaging system still uses the IO device that has the external clock function as the integrated control center, through corresponding control program (need to explain, the control program that this IO device that has the external clock function adopted can adopt current program code, it can obtain through the market purchase) controls the collection frequency of first detector, second detector and the switching linkage of a plurality of first light filters, a plurality of second light filters, controls multiunit excitation light source cooperation detector and light filter simultaneously and accomplishes work; at this moment the embodiment of the utility model provides a wide spectrum fluorescence multichannel real-time imaging system can realize the real-time separation of multiple visible light and multiple near-infrared mixed fluorescence signal, and does not have the formation of image of crosstalking.
As will be further explained in the following with reference to the accompanying drawings, the following description is only one specific embodiment of the technical solution of the present invention, and those skilled in the art can also obtain other specific embodiments according to the principles disclosed in the technical solution, wherein each optical device included in the present invention can adopt an optical device known to those skilled in the art, which can be commercially available, and the type of the optical component involved in the present invention is not specifically limited herein, and those skilled in the art can replace individual elements according to the actual situation; the control device used in the embodiment of the present invention includes but is not limited to I/O control equipment, and the control program, code, etc. involved in the control device may all adopt existing program codes, which can be obtained commercially.
Referring to fig. 1, a broad spectrum fluorescence multi-channel real-time imaging system according to an exemplary embodiment of the present invention includes: the device comprises a visible light source 1, a near-infrared laser 2, a sample stage 3, a dichroic mirror 4, a plurality of first optical filters 11, a first optical filter switching component 6, a plurality of second optical filters 12, a second optical filter switching component 5, a visible light imaging objective 13, a near-infrared imaging objective 14, an indium gallium arsenic camera 7, a silicon-based CCD 8, an I/O device 9 with an external clock function and a computer 10, wherein the visible light source 1, the near-infrared laser 2, the first optical filter switching component 6, the second optical filter switching component 5, the indium gallium arsenic camera 7 and the silicon-based CCD 8 are all connected with the I/O device 9 with the external clock function, and the I/O device 9 with the external clock function is further connected with the computer 10.
Specifically, the visible light source 1 and the near-infrared laser 2 are respectively used for providing visible light and near-infrared light with different wavelengths as excitation light, the excitation light provided by the visible light source 1 and the near-infrared laser 2 irradiates a sample to be detected on the sample stage 3, and the sample to be detected loaded with multiple probes (each probe corresponds to excitation light with a section of wavelength) is excited to emit mixed fluorescence containing multiple wavelengths of fluorescence; the mixed fluorescence is input into the dichroic mirror 4 and is separated into first fluorescence and second fluorescence with different wave bands by the dichroic mirror 4, the first fluorescence and the second fluorescence are respectively input into a first light splitting module comprising a plurality of first light filters 11 and a second light splitting module comprising a plurality of second light filters 12, the plurality of first light filters 11 are used for separating the received first fluorescence into first pulse light with a plurality of different wave bands and inputting the first pulse light into the indium gallium arsenic camera 7 through the visible light imaging objective lens 13, the plurality of second light filters 12 are used for separating the received second fluorescence into second pulse light with a plurality of different wave bands and inputting the second pulse light into the silicon-based CCD 8 through the near infrared imaging objective lens 14, and therefore co-location imaging is achieved.
Specifically, a plurality of first filters 11 are disposed on the first filter switching member 6 or a plurality of first filters 11 are connected to the first filter switching member 6, a plurality of second filters 12 are disposed on the second filter switching member 5 or a plurality of second filters 12 are connected to the second filter switching member 5, and the first filter switching member 6 and the second filter switching member 5 are further connected to an I/O device 9 having an external clock function, respectively, under the control of the I/O device 9 having the external clock function, the first filter switching member 6 and the second filter switching member 5 respectively program-switch the plurality of first filters 11 and the plurality of second filters 12, for example, in a predetermined order, so that the first fluorescence passes through the plurality of first filters 11 in a predetermined order to obtain a plurality of first pulse light signals, a plurality of second pulse light signals are obtained by passing the second fluorescence through a plurality of second filters 12 respectively in a preset order; when the first optical filters and the second optical filters are switched, the first optical filter switching component 6 and the second optical filter switching component (the first optical filter switching component and the second optical filter switching component can also be called as a programmed switching device or a programmed switcher or an optical filter disk switching device, and can adopt a commercially available programmed switcher in the prior art) 5 can generate a signal, the signal is received by the I/O device 9 and is transmitted to the InGaAs camera 7 and the silicon-based CCD 8, and the InGaAs camera 7 and the silicon-based CCD 8 receive the signal and acquire pictures; the switching frequency of the first optical filter and the second optical filter is consistent with the image acquisition frequency, and at the moment, the indium gallium arsenic camera 7 and the silicon-based CCD 8 collect multi-band and multi-probe information at a very high speed.
Specifically, the first filter 11 may be a band-pass filter, and the second filter 12 may be a band-pass near-infrared filter; the first optical filters 11 and the second optical filters 12 can be replaced to obtain first pulse optical signals and second pulse optical signals with different wavelengths; the dichroic mirror can be switched or replaced, so that the light splitting wave band can be adjusted and changed.
Specifically, the working principle and process of a broad spectrum fluorescence multi-channel real-time imaging system in a typical embodiment of the present invention at least include: the computer 10 sends out a program control instruction, and the I/O device 9 with an external clock function controls a plurality of groups of excitation light sources with different wavelengths, a plurality of filter disc switching devices and a plurality of detectors to complete the work simultaneously or in a certain time sequence, at the moment, the wide-spectrum fluorescence multi-channel real-time imaging system not only realizes 400-plus 1700nm wide-spectrum fluorescence imaging, but also realizes multi-band and multi-probe co-positioning real-time tracing, and thus, the wide-spectrum multi-channel real-time imaging is realized.
Specifically, in some more specific embodiments, the excitation light provided by the visible light source (the visible light source may be a visible light LED, a multi-wavelength visible light laser) 1 and the near-infrared laser 2 (the wavelengths of the first excitation light provided by the visible light source 1 and the second excitation light provided by the near-infrared laser 2 are both 400-; the mixed fluorescence is input into a dichroic mirror 4 and is separated into first fluorescence and second fluorescence with the wave bands of 400-900nm and 900-1700nm by the dichroic mirror 4 according to the wave bands, the first fluorescence and the second fluorescence are respectively input into a plurality of first optical filters 11 and a plurality of second optical filters 12, a first optical filter switching component 6 and a second optical filter switching component 5 are programmed to switch the plurality of first optical filters 11 and the plurality of second optical filters 12, the first fluorescence with the wave band of 900-1700nm passes through the plurality of first optical filters 11 in a certain sequence to obtain a plurality of first pulse light signals with different wave bands, and the plurality of first pulse light signals are input into an indium gallium arsenic camera 7 through a visible light imaging objective lens 13; the second fluorescence of 400-plus-900 nm waveband passes through a plurality of second optical filters 12 in a certain sequence to obtain a plurality of second pulse optical signals of different wavebands, and the plurality of second pulse optical signals are input into the silicon-based CCD 8 through the visible light imaging objective lens 17, meanwhile, the computer 10 sends out a program control instruction, and the I/O device 9 with an external clock function controls a plurality of groups of excitation light sources with different wavelengths, a plurality of optical filter disc switching devices and a plurality of detectors to complete the work at the same time or in a certain time sequence, thereby realizing the 400-plus-1700 nm wide spectrum fluorescence imaging, realizing the co-positioning real-time tracing of a plurality of wave bands and a plurality of probes, and realizing the wide spectrum multi-channel real-time imaging.
The embodiment of the utility model provides a visible light source and near-infrared laser (for example wavelength 808nm or 980nm) of wide spectrum fluorescence multichannel real-time imaging system can realize two kinds of light source simultaneous illumination through the control of the I/O device that has the external clock function; the multi-channel optical filter and the multi-channel dichroic mirror can simultaneously separate various excitation light and various fluorescent signals.
The embodiment of the utility model provides a wide spectrum fluorescence multichannel real-time imaging system uses multichannel dichroscope and a plurality of light filters of programmable switching to construct multilayer beam split structure, realize the light signal separation, wherein, first layer beam split structure (this first layer beam split structure mainly points to the dichroscope) can be separated into 400 with a garland 900nm and 900 with a garland 1700nm two wave bands with the mixed fluorescence signal that contains multiple wavelength fluorescence, second layer beam split structure includes two parallel modules, a module uses the band pass filter (being first light filter) of programmable switching as the core, separate into the first pulse light signal of a plurality of wave bands with a garland 900nm wave band with a garland 400 and send into silicon-based CCD; the other module takes a near-infrared band-pass filter (namely a second filter) which is switched in a programming way as a core, and sends the second pulse light signals of a plurality of wave bands of the fluorescence signals with the wave bands of 900-1700nm to the InGaAs camera, and a dichroic mirror and the filter in the light splitting structure are designed to be switchable, so that the wave bands of the light splitting can be adjusted and changed.
The embodiment of the utility model provides a wide spectrum fluorescence multichannel real-time imaging system, have a plurality of detectors with the optical axis, the quantum efficiency of detector is greater than 75%, have the formation of image objective of assorted with it before the detector, the detection wave band of detector is decided by the beam split wave band of beam split structure; the method specifically comprises the following steps: the silicon-based CCD respectively images a visible light wave band (400-650nm) and a near infrared first region (NIR-I650-900 nm); an InGaAs camera images a near infrared band (900-1700 nm); multiple detectors are optically coaxial to achieve detection co-localization.
The embodiment of the utility model provides a pair of real-time imaging system of wide spectrum fluorescence multichannel, the linkage of image is gathered with a plurality of detectors to a plurality of light filters switching, when a plurality of light filters switch, programmed switching device can produce a signal, and the signal is received and is defeated for the detector by the IO device, and detector received signal gathers the picture, and wherein, the two simultaneous working of IO device control that has the external clock function can realize surveying the pulse optical signal of multiband when the detector.
The embodiment of the utility model provides a pair of real-time imaging system of wide spectrum fluorescence multichannel to the IO device that has the external clock function is comprehensive control center, through corresponding control program, realizes the work is accomplished simultaneously or with certain time sequence to excitation light source, the optical filter that multiunit programming switched of the different wavelength of control multiunit, multiunit detector, realizes functions such as multichannel real-time imaging, multichannel chronogenesis formation of image, thereby realizes multiband, many probes spike simultaneously.
The embodiment of the utility model provides a real-time imaging system of wide spectrum fluorescence multichannel can realize multiple visible light, multiple near-infrared fluorescence probe common location while, real-time imaging, especially can be applied to the live body formation of image, and the live body formation of image wavelength range is 400 supplementarily for 1700 nm.
It should be understood that the above-mentioned embodiments are merely illustrative of the technical concepts and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to implement the present invention, and therefore, the protection scope of the present invention should not be limited thereby. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (13)
1. A broad spectrum fluorescence multi-channel real-time imaging system, comprising:
the excitation light source is at least used for providing first excitation light and second excitation light with different wavelengths to irradiate a sample to be detected, so that the sample to be detected is excited to emit mixed fluorescence containing multiple wavelengths of fluorescence;
the first light splitting structure is at least used for separating the mixed fluorescence into first fluorescence and second fluorescence with different wave bands and enabling the first fluorescence and the second fluorescence to be output to the second light splitting structure;
the second light splitting structure comprises a first light splitting module and a second light splitting module, wherein the first light splitting module is used for splitting the received first fluorescence into a plurality of first pulse lights with different wave bands and inputting the plurality of first pulse lights into the first detector, and the second light splitting module is used for splitting the received second fluorescence into a plurality of second pulse lights with different wave bands and inputting the plurality of second pulse lights into the second detector;
the excitation light source, the first detector and the second detector are respectively connected with the control unit.
2. The broad spectrum fluorescence multi-channel real-time imaging system of claim 1, wherein: the first light splitting structure includes a dichroic mirror.
3. The broad spectrum fluorescence multi-channel real-time imaging system of claim 2, wherein: the dichroic mirror is a switchable plurality of dichroic mirrors.
4. The broad spectrum fluorescence multi-channel real-time imaging system of claim 1, wherein: the first light splitting module comprises a plurality of first optical filters, the first optical filters are respectively matched with the first pulse lights with the different wave bands, and the first fluorescence respectively passes through the first optical filters to obtain the first pulse lights with the different wave bands.
5. The broad spectrum fluorescence multi-channel real-time imaging system of claim 4, wherein: the plurality of first optical filters are connected with a first optical filter switching component, the first optical filter switching component is connected with the control unit, and the first optical filter switching component can switch the positions of the plurality of first optical filters according to a preset sequence, so that received first fluorescence respectively passes through the plurality of first optical filters in sequence, and then first pulse light of a plurality of different wave bands is obtained.
6. The broad spectrum fluorescence multi-channel real-time imaging system of claim 1, wherein: the second light splitting module comprises a plurality of second optical filters, the second optical filters are respectively matched with the second pulse lights with the different wave bands, and the second fluorescence respectively passes through the second optical filters to obtain the second pulse lights with the different wave bands.
7. The broad spectrum fluorescence multi-channel real-time imaging system of claim 6, wherein: the second optical filter switching components are connected with the control unit and can switch the positions of the second optical filters according to a preset sequence, so that the received second fluorescent light respectively passes through the second optical filters in sequence, and second pulsed light with a plurality of different wave bands is obtained.
8. The broad spectrum fluorescence multi-channel real-time imaging system of claim 6, wherein: the first pulse light and the second pulse light are respectively input into the first detector and the second detector through the first imaging objective lens and the second imaging objective lens.
9. The broad spectrum fluorescence multi-channel real-time imaging system of claim 1 or 8, wherein: the first detector and the second detector are arranged in an optical coaxial mode, the first detector comprises an indium gallium arsenic camera, and the second detector comprises a silicon-based CCD.
10. The broad spectrum fluorescence multi-channel real-time imaging system of claim 1, wherein: the excitation light source comprises a first excitation light source and a second excitation light source which are respectively used for providing first excitation light and second excitation light, the first excitation light source comprises a visible light source, and the second excitation light source comprises a near-infrared laser.
11. The broad spectrum fluorescence multi-channel real-time imaging system of claim 1, wherein: the wavelength of the mixed fluorescence is 400-1700 nm.
12. The broad spectrum fluorescence multi-channel real-time imaging system of claim 1, wherein: the control unit includes an I/O device having a clock function.
13. The broad spectrum fluorescence multi-channel real-time imaging system of claim 12, wherein: the I/O device is also connected with a computer.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113143207A (en) * | 2021-03-09 | 2021-07-23 | 复旦大学 | Ultra-wide waveband double-channel living body optical imaging system |
| CN113576415A (en) * | 2021-08-04 | 2021-11-02 | 广东光声医疗科技有限公司 | Four-mode photoacoustic, ultrasonic, fluorescence and optical imaging microscope system |
| CN113847987A (en) * | 2021-11-16 | 2021-12-28 | 电子科技大学 | Infrared and visible light integrated wide spectrum fluorescence measurement method |
| TWI814365B (en) * | 2022-04-29 | 2023-09-01 | 由田新技股份有限公司 | Optical inspection system based on laser light source and laser optical system |
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2020
- 2020-05-27 CN CN202020920903.2U patent/CN212514276U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113143207A (en) * | 2021-03-09 | 2021-07-23 | 复旦大学 | Ultra-wide waveband double-channel living body optical imaging system |
| CN113576415A (en) * | 2021-08-04 | 2021-11-02 | 广东光声医疗科技有限公司 | Four-mode photoacoustic, ultrasonic, fluorescence and optical imaging microscope system |
| CN113847987A (en) * | 2021-11-16 | 2021-12-28 | 电子科技大学 | Infrared and visible light integrated wide spectrum fluorescence measurement method |
| CN113847987B (en) * | 2021-11-16 | 2023-12-15 | 电子科技大学 | Infrared and visible light integrated wide-spectrum fluorescence measurement method |
| TWI814365B (en) * | 2022-04-29 | 2023-09-01 | 由田新技股份有限公司 | Optical inspection system based on laser light source and laser optical system |
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