JPWO2020126602A5 - - Google Patents
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- Publication number
- JPWO2020126602A5 JPWO2020126602A5 JP2020572480A JP2020572480A JPWO2020126602A5 JP WO2020126602 A5 JPWO2020126602 A5 JP WO2020126602A5 JP 2020572480 A JP2020572480 A JP 2020572480A JP 2020572480 A JP2020572480 A JP 2020572480A JP WO2020126602 A5 JPWO2020126602 A5 JP WO2020126602A5
- Authority
- JP
- Japan
- Prior art keywords
- strand
- amplification
- site
- nucleotides
- complementary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 230000003321 amplification Effects 0.000 claims 25
- 238000003199 nucleic acid amplification method Methods 0.000 claims 25
- 238000012163 sequencing technique Methods 0.000 claims 25
- 108091093088 Amplicon Proteins 0.000 claims 21
- 108020004707 nucleic acids Proteins 0.000 claims 21
- 150000007523 nucleic acids Chemical class 0.000 claims 21
- 102000039446 nucleic acids Human genes 0.000 claims 21
- 239000002773 nucleotide Substances 0.000 claims 20
- 125000003729 nucleotide group Chemical group 0.000 claims 20
- 238000000034 method Methods 0.000 claims 19
- 230000000295 complement effect Effects 0.000 claims 17
- 238000003776 cleavage reaction Methods 0.000 claims 14
- 230000007017 scission Effects 0.000 claims 14
- 238000006243 chemical reaction Methods 0.000 claims 11
- 208000035657 Abasia Diseases 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 238000006073 displacement reaction Methods 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- UBKVUFQGVWHZIR-UHFFFAOYSA-N 8-oxoguanine Chemical compound O=C1NC(N)=NC2=NC(=O)N=C21 UBKVUFQGVWHZIR-UHFFFAOYSA-N 0.000 claims 1
- 108020001738 DNA Glycosylase Proteins 0.000 claims 1
- 102000028381 DNA glycosylase Human genes 0.000 claims 1
- 102000004099 Deoxyribonuclease (Pyrimidine Dimer) Human genes 0.000 claims 1
- 108010082610 Deoxyribonuclease (Pyrimidine Dimer) Proteins 0.000 claims 1
- 102000004533 Endonucleases Human genes 0.000 claims 1
- 108010042407 Endonucleases Proteins 0.000 claims 1
- 102000004317 Lyases Human genes 0.000 claims 1
- 108090000856 Lyases Proteins 0.000 claims 1
- 101710147059 Nicking endonuclease Proteins 0.000 claims 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 1
- 239000003398 denaturant Substances 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 108010055863 gene b exonuclease Proteins 0.000 claims 1
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 claims 1
- 108010068698 spleen exonuclease Proteins 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862781273P | 2018-12-18 | 2018-12-18 | |
US62/781,273 | 2018-12-18 | ||
PCT/EP2019/084183 WO2020126602A1 (en) | 2018-12-18 | 2019-12-09 | Methods and compositions for paired end sequencing using a single surface primer |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2022513546A JP2022513546A (ja) | 2022-02-09 |
JPWO2020126602A5 true JPWO2020126602A5 (enrdf_load_stackoverflow) | 2022-10-05 |
JP7587425B2 JP7587425B2 (ja) | 2024-11-20 |
Family
ID=68887016
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2020572480A Active JP7587425B2 (ja) | 2018-12-18 | 2019-12-09 | 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物 |
Country Status (9)
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112654718B (zh) | 2018-12-05 | 2025-07-22 | 伊卢米纳剑桥有限公司 | 通过桥式扩增进行簇生成的方法和组合物 |
US12359193B2 (en) | 2022-03-04 | 2025-07-15 | Element Biosciences, Inc. | Single-stranded splint strands and methods of use |
US12371743B2 (en) | 2022-03-04 | 2025-07-29 | Element Biosciences, Inc. | Double-stranded splint adaptors and methods of use |
GB2623234A (en) * | 2021-06-17 | 2024-04-10 | Element Biosciences Inc | Compositions and methods for pairwise sequencing |
US11859241B2 (en) | 2021-06-17 | 2024-01-02 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
WO2022266470A1 (en) * | 2021-06-17 | 2022-12-22 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
US11236388B1 (en) | 2021-06-17 | 2022-02-01 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
CN118272513A (zh) * | 2022-01-28 | 2024-07-02 | 赛纳生物科技(北京)有限公司 | 一种芯片表面双端扩增测序的方法 |
CN119082279A (zh) * | 2022-01-28 | 2024-12-06 | 赛纳生物科技(北京)有限公司 | 一种固相介质表面双端扩增测序的方法 |
WO2023186872A1 (en) * | 2022-03-30 | 2023-10-05 | Illumina Cambridge Limited | Methods for chemical cleavage of surface-bound polynucleotides |
US12365892B2 (en) | 2022-09-12 | 2025-07-22 | Element Biosciences, Inc. | Double-stranded splint adaptors with universal long splint strands and methods of use |
Family Cites Families (70)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8810400D0 (en) | 1988-05-03 | 1988-06-08 | Southern E | Analysing polynucleotide sequences |
EP0450060A1 (en) | 1989-10-26 | 1991-10-09 | Sri International | Dna sequencing |
US5552278A (en) | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
US6023540A (en) | 1997-03-14 | 2000-02-08 | Trustees Of Tufts College | Fiber optic sensor with encoded microspheres |
US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
ES2563643T3 (es) | 1997-04-01 | 2016-03-15 | Illumina Cambridge Limited | Método de secuenciación de ácido nucleico |
DE19812103A1 (de) | 1998-03-19 | 1999-09-23 | Bernauer Annette | Verfahren zur Synthese von Nucleinsäuremolekülen |
AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
US20050244870A1 (en) | 1999-04-20 | 2005-11-03 | Illumina, Inc. | Nucleic acid sequencing using microsphere arrays |
US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
WO2000063437A2 (en) | 1999-04-20 | 2000-10-26 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
JP2003521252A (ja) | 2000-02-07 | 2003-07-15 | イルミナ インコーポレイテッド | ユニバーサルプライミングを用いる核酸検出方法 |
US6913884B2 (en) | 2001-08-16 | 2005-07-05 | Illumina, Inc. | Compositions and methods for repetitive use of genomic DNA |
US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
CA2415897A1 (en) | 2000-07-07 | 2002-01-17 | Susan H. Hardin | Real-time sequence determination |
US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
JP2006509040A (ja) | 2002-08-23 | 2006-03-16 | ソレックサ リミテッド | 修飾されたヌクレオチド |
US7595883B1 (en) | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
DE20214753U1 (de) | 2002-09-24 | 2002-12-12 | Hando Handels Ges. M.B.H., Graz | Pharmazeutische Zusammensetzung zur Behandlung und/oder Prävention einer Lichtdermatose |
CA2528577C (en) | 2003-06-20 | 2012-08-07 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
EP3673986A1 (en) | 2004-01-07 | 2020-07-01 | Illumina Cambridge Limited | Improvements in or relating to molecular arrays |
US7315019B2 (en) | 2004-09-17 | 2008-01-01 | Pacific Biosciences Of California, Inc. | Arrays of optical confinements and uses thereof |
WO2006064199A1 (en) | 2004-12-13 | 2006-06-22 | Solexa Limited | Improved method of nucleotide detection |
GB0514910D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Method for sequencing a polynucleotide template |
GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
US8192930B2 (en) | 2006-02-08 | 2012-06-05 | Illumina Cambridge Limited | Method for sequencing a polynucleotide template |
EP4105644A3 (en) | 2006-03-31 | 2022-12-28 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
US8071308B2 (en) | 2006-05-04 | 2011-12-06 | Alere San Diego, Inc. | Recombinase polymerase amplification |
US7754429B2 (en) | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
WO2008051530A2 (en) | 2006-10-23 | 2008-05-02 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
EP2762872B1 (en) | 2006-12-14 | 2015-09-16 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
WO2008093098A2 (en) | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
US8198023B2 (en) | 2008-08-05 | 2012-06-12 | Pacific Biosciences Of California, Inc. | Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase |
US8586310B2 (en) | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US9029103B2 (en) | 2010-08-27 | 2015-05-12 | Illumina Cambridge Limited | Methods for sequencing polynucleotides |
WO2012058096A1 (en) | 2010-10-27 | 2012-05-03 | Illumina, Inc. | Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same |
EP3147374B1 (en) | 2010-12-17 | 2019-01-30 | Life Technologies Corporation | Methods for nucleic acid amplification |
US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
EP2718465B1 (en) | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Method of making an analyte array |
US9139874B2 (en) * | 2011-07-07 | 2015-09-22 | Life Technologies Corporation | Bi-directional sequencing compositions and methods |
RS62530B1 (sr) | 2011-09-23 | 2021-11-30 | Illumina Inc | Sastavi za sekvenciranje nukleinskih kiselina |
US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
JP2015526092A (ja) * | 2012-08-24 | 2015-09-10 | ライフ テクノロジーズ コーポレーション | 核酸ペアエンド配列決定のための方法、組成物、システム、装置、およびキット |
US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
US9677132B2 (en) | 2014-01-16 | 2017-06-13 | Illumina, Inc. | Polynucleotide modification on solid support |
WO2016054096A1 (en) | 2014-09-30 | 2016-04-07 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
WO2016075204A1 (en) | 2014-11-11 | 2016-05-19 | Illumina, Inc. | Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid |
DK3256604T3 (da) | 2015-02-10 | 2020-05-25 | Illumina Inc | Fremgangsmåder og sammensætninger til analyse af cellebestanddele |
CN117822128A (zh) | 2016-07-22 | 2024-04-05 | 俄勒冈健康与科学大学 | 单细胞全基因组文库及制备其的组合索引方法 |
DK3615690T3 (da) | 2017-04-23 | 2021-11-15 | Illumina Cambridge Ltd | Sammensætninger og fremgangsmåder til forbedring af prøveidentificering i indekserede nukleinsyrebiblioteker |
CN110785497A (zh) | 2017-04-23 | 2020-02-11 | 伊鲁米纳剑桥有限公司 | 用于改进编索引的核酸文库中的样品鉴定的组合物和方法 |
EP3913053A1 (en) | 2017-04-23 | 2021-11-24 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
SG11201909394PA (en) | 2017-04-23 | 2019-11-28 | Illumina Inc | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
CN112654718B (zh) | 2018-12-05 | 2025-07-22 | 伊卢米纳剑桥有限公司 | 通过桥式扩增进行簇生成的方法和组合物 |
-
2019
- 2019-12-09 WO PCT/EP2019/084183 patent/WO2020126602A1/en unknown
- 2019-12-09 KR KR1020207037096A patent/KR20210104554A/ko active Pending
- 2019-12-09 EP EP19820701.1A patent/EP3899041A1/en active Pending
- 2019-12-09 JP JP2020572480A patent/JP7587425B2/ja active Active
- 2019-12-09 AU AU2019411272A patent/AU2019411272B2/en active Active
- 2019-12-09 SG SG11202012807YA patent/SG11202012807YA/en unknown
- 2019-12-09 CN CN201980042671.5A patent/CN112739830A/zh active Pending
- 2019-12-09 CA CA3103633A patent/CA3103633A1/en active Pending
- 2019-12-17 US US16/717,303 patent/US11634765B2/en active Active
-
2022
- 2022-12-27 US US18/089,352 patent/US20230137106A1/en active Pending
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