JPWO2020045406A1 - An oyster flavor reduction inhibitor, an aqueous solution for holding live oysters, and a method for producing oysters with suppressed flavor deterioration. - Google Patents

An oyster flavor reduction inhibitor, an aqueous solution for holding live oysters, and a method for producing oysters with suppressed flavor deterioration. Download PDF

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JPWO2020045406A1
JPWO2020045406A1 JP2020539480A JP2020539480A JPWO2020045406A1 JP WO2020045406 A1 JPWO2020045406 A1 JP WO2020045406A1 JP 2020539480 A JP2020539480 A JP 2020539480A JP 2020539480 A JP2020539480 A JP 2020539480A JP WO2020045406 A1 JPWO2020045406 A1 JP WO2020045406A1
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敏弘 藤本
敏弘 藤本
綾子 藤原
綾子 藤原
秀行 玉井
秀行 玉井
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/02Preserving by means of inorganic salts
    • A23B4/023Preserving by means of inorganic salts by kitchen salt or mixtures thereof with inorganic or organic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/40Shell-fish

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Abstract

エノキタケ抽出物、酵母抽出物及び大麦抽出物からなる群より選ばれる少なくとも1種の抽出物を含む、牡蠣の風味低下抑制剤。前記抽出物を、前記牡蠣の風味低下抑制剤の乾燥重量全体中、乾燥重量で50〜100重量%含むことが好ましい。前記牡蠣の風味低下抑制剤を用いて、活牡蠣の保持用水溶液を提供することができる。An oyster flavor reduction inhibitor containing at least one extract selected from the group consisting of enokitake extract, yeast extract and barley extract. It is preferable that the extract is contained in an amount of 50 to 100% by weight based on the dry weight of the oyster flavor reduction inhibitor. An aqueous solution for holding live oysters can be provided by using the oyster flavor reduction inhibitor.

Description

本発明は、牡蠣の風味低下抑制剤、活牡蠣の保持用水溶液、及び、風味低下が抑制された牡蠣の製造方法に関する。 The present invention relates to an oyster flavor reduction inhibitor, an aqueous solution for holding live oysters, and a method for producing oysters in which flavor deterioration is suppressed.

牡蠣は海のミルクといわれ、ミルキーで濃厚な風味をもち、栄養価も高いため人気がある。しかしながら、水揚げ後の生牡蠣を冷蔵してから輸送または保存すると、その輸送または保存中に牡蠣の風味が徐々に低下するという問題があり、風味を保持したまま輸送できる地域が限られていた。また、水槽に海水を入れて酸素を供給しながら魚介類を生きたまま定温輸送する方法もあるが、この方法では温度管理や酸素管理に大型の装置を必要とし、経済効率の点で望ましいものではなかった。さらに、水揚げ直後の生牡蠣を冷凍してから輸送または保存する方法も一般的であるが、この方法によると、冷凍中の水分昇華や、解凍時のドリップなどにより牡蠣の風味が著しく損なわれる、いわゆる冷凍障害が発生するという問題があった。 Oysters are said to be sea milk and are popular because they have a milky, rich flavor and high nutritional value. However, if raw oysters after landing are refrigerated and then transported or stored, there is a problem that the flavor of the oysters gradually deteriorates during the transportation or storage, and the area where the oysters can be transported while maintaining the flavor is limited. There is also a method of transporting seafood alive at a constant temperature by putting seawater in a water tank and supplying oxygen, but this method requires a large device for temperature control and oxygen control, which is desirable in terms of economic efficiency. It wasn't. Furthermore, a method of freezing raw oysters immediately after landing and then transporting or storing them is also common, but according to this method, the flavor of oysters is significantly impaired due to sublimation of water during freezing and drip during thawing. There was a problem that so-called freezing failure occurred.

特許文献1では、生鮮魚介類の鮮度を保持することを目的に、α,α−トレハロース、クエン酸等の有機酸、及び/又は、ポリフェノールを有効成分として含有する魚介類の鮮度保持剤が開示されている。しかし、この鮮度保持剤を牡蠣に適用すると、鮮度保持剤に含まれる成分の風味や色が牡蠣に付着して、牡蠣本来の風味や色調が損なわれる問題がある。また、特許文献2では、貝類を短時間で軟化させて食感および食味を改善するために、アルカリ性物質と、乳酸ナトリウムまたは乳酸カリウムとを含む品質改良剤を貝類に接触させる方法が開示されている。しかし、当該方法は、貝類を軟化させることを目的としたものであり、牡蠣の水揚げ後に進行し得る牡蠣の風味の低下を抑制しようとするものではない。 Patent Document 1 discloses a freshness-preserving agent for seafood containing an organic acid such as α, α-trehalose, citric acid, and / or a polyphenol as an active ingredient for the purpose of maintaining the freshness of fresh seafood. Has been done. However, when this freshness preserving agent is applied to oysters, there is a problem that the flavor and color of the components contained in the freshness preserving agent adhere to the oysters and the original flavor and color tone of the oysters are impaired. Further, Patent Document 2 discloses a method in which a quality improving agent containing an alkaline substance and sodium lactate or potassium lactate is brought into contact with the shellfish in order to soften the shellfish in a short time and improve the texture and taste. There is. However, the method is intended to soften shellfish, and is not intended to suppress the deterioration of oyster flavor that may progress after landing of oysters.

特開2003−225047号公報Japanese Unexamined Patent Publication No. 2003-225847 特開2010−154799号公報JP-A-2010-154799

本発明の目的は、牡蠣の水揚げ後に進行し得る牡蠣の風味の低下を抑制して、本来のミルキーで濃厚な風味を保持した生牡蠣を提供するために使用される、牡蠣の風味低下抑制剤、活牡蠣の保持用水溶液、及び、風味低下が抑制された牡蠣の製造方法を提供することである。 An object of the present invention is an oyster flavor reduction inhibitor used to suppress the deterioration of oyster flavor that may progress after landing of oysters and to provide raw oysters that retain the original milky and rich flavor. , An aqueous solution for holding live oysters, and a method for producing oysters in which deterioration of flavor is suppressed.

本発明者らは上記課題を解決するために鋭意研究を重ねた結果、特定の抽出物を特定量含有した特定の塩化ナトリウム濃度の水溶液に、活牡蠣を特定時間保持することで、その後の輸送または保存時に生じ得る牡蠣の風味の低下を抑制できることを見出し、本発明を完成するに至った。 As a result of diligent research to solve the above problems, the present inventors held live oysters in an aqueous solution containing a specific amount of a specific extract and a specific sodium chloride concentration for a specific time, thereby transporting the live oysters thereafter. Alternatively, they have found that the deterioration of the flavor of oysters that may occur during storage can be suppressed, and have completed the present invention.

即ち、本発明の第一は、エノキタケ抽出物、酵母抽出物及び大麦抽出物からなる群より選ばれる少なくとも1種の抽出物を含む、牡蠣の風味低下抑制剤に関する。当該牡蠣の風味低下抑制剤は、前記抽出物を、前記牡蠣の風味低下抑制剤の乾燥重量全体中、乾燥重量で50〜100重量%含むことが好ましい。
本発明の第二は、エノキタケ抽出物、酵母抽出物及び大麦抽出物からなる群より選ばれる少なくとも1種の抽出物を固形分で0.1〜800ppm含有し、塩化ナトリウム濃度が2〜3.5重量%である、活牡蠣の保持用水溶液に関する。当該活牡蠣の保持用水溶液は、前記牡蠣の風味低下抑制剤を用いたものであってよい。
本発明の第三は、温度が1〜25℃の範囲にある前記水溶液中に、酸素が供給された状態で10〜15000分間保持された活牡蠣に関する。
本発明の第四は、前記活牡蠣が冷蔵または冷凍された生牡蠣に関する。
本発明の第五は、エノキタケ抽出物、酵母抽出物及び大麦抽出物からなる群より選ばれる少なくとも1種の抽出物を固形分で0.1〜800ppm含有し、塩化ナトリウム濃度が2〜3.5重量%、温度が1〜25℃の範囲にある水溶液中に、酸素が供給された状態で活牡蠣を10〜15000分間保持することを特徴とする、風味低下が抑制された牡蠣の製造方法に関する。
That is, the first aspect of the present invention relates to an oyster flavor reduction inhibitor containing at least one extract selected from the group consisting of enokitake mushroom extract, yeast extract and barley extract. The oyster flavor reduction inhibitor preferably contains the extract in an amount of 50 to 100% by weight based on the dry weight of the oyster flavor reduction inhibitor.
The second aspect of the present invention is to contain at least one extract selected from the group consisting of an enokitake extract, a yeast extract and a barley extract in a solid content of 0.1 to 800 ppm and a sodium chloride concentration of 2 to 3. It relates to an aqueous solution for holding live barley, which is 5% by weight. The aqueous solution for holding the live oyster may be one using the agent for suppressing the flavor deterioration of the oyster.
A third aspect of the present invention relates to live oysters that are kept in the aqueous solution having a temperature in the range of 1 to 25 ° C. for 10 to 15,000 minutes while being supplied with oxygen.
The fourth aspect of the present invention relates to raw oysters in which the live oysters are refrigerated or frozen.
Fifth of the present invention contains at least one extract selected from the group consisting of enokitake extract, yeast extract and barley extract in terms of solid content of 0.1 to 800 ppm and a sodium chloride concentration of 2 to 3. A method for producing an oyster with suppressed flavor deterioration, which comprises holding a live oyster for 10 to 15,000 minutes in a state where oxygen is supplied in an aqueous solution having a temperature in the range of 5% by weight and a temperature of 1 to 25 ° C. Regarding.

本発明に従えば、牡蠣の水揚げ後に進行し得る牡蠣の風味の低下を抑制して、本来のミルキーで濃厚な風味を保持した生牡蠣を提供するために使用される、牡蠣の風味低下抑制剤、活牡蠣の保持用水溶液、及び、風味低下が抑制された牡蠣の製造方法を提供することができる。本発明によると、生牡蠣を冷蔵または冷凍した場合において牡蠣の風味の低下を抑制することができ、また、冷凍した場合であっても、冷凍障害を抑制することもできる。また、風味低下抑制剤に由来する風味や色が生牡蠣に付着することも回避できる。本発明によると、牡蠣の風味の低下が抑制されるので、本来のミルキーで濃厚な風味を保持した生牡蠣の輸送可能なエリアを拡大することができる。 According to the present invention, an oyster flavor reduction inhibitor used to suppress the deterioration of oyster flavor that may progress after landing of oysters and to provide raw oysters that retain the original milky and rich flavor. , An aqueous solution for holding live oysters, and a method for producing oysters in which flavor deterioration is suppressed can be provided. According to the present invention, it is possible to suppress a decrease in the flavor of oysters when the raw oysters are refrigerated or frozen, and it is also possible to suppress a freezing disorder even when the raw oysters are frozen. In addition, it is possible to prevent the flavor and color derived from the flavor reduction inhibitor from adhering to the raw oysters. According to the present invention, since the deterioration of the flavor of oysters is suppressed, it is possible to expand the transportable area of raw oysters that retain the original milky and rich flavor.

以下、本発明につき、さらに詳細に説明する。本発明の牡蠣の風味低下抑制剤は、牡蠣の水揚げ後、特に生牡蠣の冷蔵または冷凍中に生じ得る牡蠣のミルキーで濃厚な風味の低下を抑制するためのもので、特定の抽出物を含有する。本発明の牡蠣の風味低下抑制剤は、塩水に配合して使用されるもので、生牡蠣を冷蔵または冷凍する前に、本発明の牡蠣の風味低下抑制剤を含む塩水で活牡蠣を処理することで本発明の目的を達成することができる。 Hereinafter, the present invention will be described in more detail. The oyster flavor reduction inhibitor of the present invention is for suppressing the milky and rich flavor reduction of oysters that may occur after landing of oysters, especially during refrigeration or freezing of raw oysters, and contains a specific extract. To do. The oyster flavor reduction inhibitor of the present invention is used by blending with salt water, and the live oyster is treated with salt water containing the oyster flavor reduction inhibitor of the present invention before refrigerating or freezing the raw oyster. This makes it possible to achieve the object of the present invention.

本発明の牡蠣の風味低下抑制剤で使用する抽出物は、エノキタケ抽出物、酵母抽出物及び大麦抽出物からなる群より選ばれる少なくとも1種の抽出物である。 The extract used in the oyster flavor reduction inhibitor of the present invention is at least one extract selected from the group consisting of enokitake mushroom extract, yeast extract and barley extract.

前記エノキタケ抽出物としては特に限定されないが、例えば、エノキタケの子実体を熱水及び/又はアルコール等の抽出溶媒で抽出した抽出物、酵素やアルカリ性の溶媒でエノキタケの子実体を分解してから熱水やアルコール等の抽出溶媒で抽出した抽出物、更にはこれら抽出物を濃縮した濃縮液、前記抽出物または前記濃縮液を乾燥して得られた粉末等が挙げられる。これら抽出物は、消費者の安心安全志向から、水や天然由来エタノール以外の溶媒を用いない抽出物が好ましい。但し、コスト面を考えると、水がより好ましい。 The enokitake extract is not particularly limited, but for example, an extract obtained by extracting the offspring of enokitake with an extraction solvent such as hot water and / or alcohol, or after decomposing the offspring of enokitake with an enzyme or an alkaline solvent, heat is applied. Examples thereof include an extract extracted with an extraction solvent such as water or alcohol, a concentrated solution obtained by concentrating these extracts, the extract or a powder obtained by drying the concentrated solution, and the like. These extracts are preferably extracts that do not use a solvent other than water or naturally-derived ethanol from the viewpoint of consumers' safety and security. However, considering the cost, water is more preferable.

抽出時の抽出溶媒の温度は、抽出溶媒の種類によって好適な温度幅が変動し得るが、一般に、抽出溶媒の大気圧下での沸点(℃)−20℃〜抽出溶媒の大気圧下での沸点(℃)が好ましい。抽出時の抽出溶媒の温度が、該抽出溶媒の大気圧下での沸点(℃)−20℃より低いと、牡蠣の風味低下抑制効果が弱くなる場合があり、大気圧下での沸点(℃)より高くなると、特殊な加圧設備が必要となるため、抽出方法が複雑になる場合がある。 The temperature of the extraction solvent at the time of extraction may vary in a suitable temperature range depending on the type of the extraction solvent, but generally, the boiling point (° C.) of the extraction solvent under atmospheric pressure is -20 ° C to that of the extraction solvent under atmospheric pressure. Boiling point (° C.) is preferred. If the temperature of the extraction solvent at the time of extraction is lower than the boiling point (° C.) -20 ° C of the extraction solvent under atmospheric pressure, the effect of suppressing the flavor deterioration of oysters may be weakened, and the boiling point (° C.) under atmospheric pressure may be weakened. ), The extraction method may be complicated because special pressurizing equipment is required.

前記エノキタケは、タマバリタケ科のキノコの一種であるFlammulina velutipes種のことをいう。特に限定されないが、人工的に栽培した白色かつもやし状の市販エノキタケを使用することができる。かかる市販エノキタケは、一般に食用とされており、容易に入手可能である。 The enokitake refers to a species of Flameluina velutipes, which is a kind of mushroom of the family Physalacriaceae. Although not particularly limited, artificially cultivated white and bean sprout-like commercially available enokitake mushrooms can be used. Such commercially available enokitake mushrooms are generally edible and easily available.

前記酵母抽出物としては特に限定されないが、例えば、酵母を熱水やアルコール等の抽出溶媒で抽出した抽出物、酵素やアルカリ性の溶媒で酵母を分解してから熱水やアルコール等の抽出溶媒で抽出した抽出物、更にはこれら抽出物を濃縮した濃縮液、前記抽出物または前記濃縮液を乾燥して得られた粉末等が挙げられる。これら抽出物は、消費者の安心安全志向から、水や天然由来エタノール以外の溶媒を用いない抽出物が好ましい。但し、コスト面を考えると、水がより好ましい。 The yeast extract is not particularly limited, but for example, an extract obtained by extracting yeast with an extraction solvent such as hot water or alcohol, or an extract obtained by decomposing yeast with an enzyme or an alkaline solvent and then using an extraction solvent such as hot water or alcohol. Examples thereof include the extracted extract, a concentrated solution obtained by concentrating these extracts, the extract or a powder obtained by drying the concentrated solution, and the like. These extracts are preferably extracts that do not use a solvent other than water or naturally-derived ethanol from the viewpoint of consumers' safety and security. However, considering the cost, water is more preferable.

抽出時の抽出溶媒の温度は、抽出溶媒の種類によって好適な温度幅が変動し得るが、一般に、抽出溶媒の大気圧下での沸点(℃)−20℃〜抽出溶媒の大気圧下での沸点(℃)が好ましい。抽出時の抽出溶媒の温度が、該抽出溶媒の大気圧下での沸点(℃)−20℃より低いと、牡蠣の風味低下抑制効果が弱くなる場合があり、大気圧下での沸点(℃)より高くなると、特殊な加圧設備が必要となるため、抽出方法が複雑になる場合がある。 The temperature of the extraction solvent at the time of extraction may vary in a suitable temperature range depending on the type of the extraction solvent, but is generally from the boiling point (° C.) of the extraction solvent under atmospheric pressure (° C.) to -20 ° C to the atmospheric pressure of the extraction solvent. Boiling point (° C.) is preferred. If the temperature of the extraction solvent at the time of extraction is lower than the boiling point (° C.) -20 ° C of the extraction solvent under atmospheric pressure, the effect of suppressing the flavor deterioration of oysters may be weakened, and the boiling point (° C.) under atmospheric pressure may be weakened. ), The extraction method may be complicated because special pressurizing equipment is required.

前記酵母は、嫌気環境下で発酵する際に、糖を資化してアルコールを生成する微生物のことをいう。具体例として、サッカロミセス(Saccharomyces)属に属する酵母や、キャンディダ(Candida)属に属する酵母等が挙げられるが、特に限定されない。食経験が豊富である観点から、前記酵母は、サッカロミセス・セレビジエ(Saccharomyces cerevisiae)であってもよく、研究等で知見が多い観点から、前記酵母は、キャンディダ・ユーティリス(Candida utilis)であってもよい。 The yeast refers to a microorganism that assimilate sugar to produce alcohol when fermented in an anaerobic environment. Specific examples include yeasts belonging to the genus Saccharomyces, yeasts belonging to the genus Candida, and the like, but are not particularly limited. From the viewpoint of having abundant eating experience, the yeast may be Saccharomyces cerevisiae, and from the viewpoint of much knowledge in research and the like, the yeast is Candida utilis. You may.

前記大麦抽出物としては特に限定されないが、例えば、大麦を熱水やアルコール等の抽出溶媒で抽出した抽出物、酵素やアルカリ性の溶媒で大麦を分解してから熱水やアルコール等の抽出溶媒で抽出した抽出物、更にはこれら抽出物を濃縮した濃縮液、前記抽出物または前記濃縮液を乾燥して得られた粉末等が挙げられる。これら抽出物は、消費者の安心安全志向から、水や天然由来エタノール以外の溶媒を用いない抽出物が好ましい。但し、コスト面を考えると、水がより好ましい。 The barley extract is not particularly limited, but for example, an extract obtained by extracting barley with an extraction solvent such as hot water or alcohol, or decomposing barley with an enzyme or an alkaline solvent and then using an extraction solvent such as hot water or alcohol. Examples thereof include the extracted extract, a concentrated solution obtained by concentrating these extracts, the extract or a powder obtained by drying the concentrated solution, and the like. These extracts are preferably extracts that do not use a solvent other than water or naturally-derived ethanol from the viewpoint of consumers' safety and security. However, considering the cost, water is more preferable.

抽出時の抽出溶媒の温度は、抽出溶媒の種類によって好適な温度幅が変動し得るが、一般に、抽出溶媒の大気圧下での沸点(℃)−20℃〜抽出溶媒の大気圧下での沸点(℃)が好ましい。抽出時の抽出溶媒の温度が、該抽出溶媒の大気圧下での沸点(℃)−20℃より低いと、牡蠣の風味低下抑制効果が弱くなる場合があり、大気圧下での沸点(℃)より高くなると、特殊な加圧設備が必要となるため、抽出方法が複雑になる場合がある。 The temperature of the extraction solvent at the time of extraction may vary in a suitable temperature range depending on the type of the extraction solvent, but generally, the boiling point (° C.) of the extraction solvent under atmospheric pressure is -20 ° C to that of the extraction solvent under atmospheric pressure. Boiling point (° C.) is preferred. If the temperature of the extraction solvent at the time of extraction is lower than the boiling point (° C.) -20 ° C of the extraction solvent under atmospheric pressure, the effect of suppressing the flavor deterioration of oysters may be weakened, and the boiling point (° C.) under atmospheric pressure may be weakened. ), The extraction method may be complicated because special pressurizing equipment is required.

前記大麦は、中央アジア原産のイネ科の植物で、学名をHordeum vulgareといい、二条大麦と六条大麦、皮麦とはだか麦、うるち種ともち種に分類されるものをいう。 The barley is a plant of the Gramineae family native to Central Asia, and its scientific name is Hordeum vulgare, and it is classified into two-row barley and six-row barley, skin barley and hordeum, and glutinous and mochi.

本発明の牡蠣の風味低下抑制剤は、エノキタケ抽出物、酵母抽出物及び大麦抽出物からなる群より選ばれる少なくとも1種の抽出物を、該風味低下抑制剤の乾燥重量全体に対し乾燥重量で50〜100重量%含有することが好ましい。より好ましくは70〜100重量%であり、さらに好ましくは90〜100重量%である。50重量%よりも少ないと牡蠣の風味低下抑制効果を発揮できない場合がある。本発明の牡蠣の風味低下抑制剤に含まれ得る前記抽出物以外の固形分としては特に限定されず、天然由来の固形分であれば何を含有しても良く、例えば、デキストリン、澱粉、乳糖などが挙げられる。 The oyster flavor reduction inhibitor of the present invention is obtained by adding at least one extract selected from the group consisting of enokitake extract, yeast extract and barley extract by dry weight to the total dry weight of the flavor reduction inhibitor. It is preferably contained in an amount of 50 to 100% by weight. It is more preferably 70 to 100% by weight, still more preferably 90 to 100% by weight. If it is less than 50% by weight, the effect of suppressing the deterioration of oyster flavor may not be exhibited. The solid content other than the extract that can be contained in the oyster flavor reduction inhibitor of the present invention is not particularly limited, and any naturally derived solid content may be contained, for example, dextrin, starch, lactose. And so on.

本発明の牡蠣の風味低下抑制剤の形状は特に限定されず、水溶液等の液体の形状であってもよいし、粉末状、顆粒状、ブロック状等の固形の形状であってもよい。 The shape of the oyster flavor reduction inhibitor of the present invention is not particularly limited, and may be a liquid shape such as an aqueous solution, or a solid shape such as powder, granule, or block.

本発明の牡蠣の風味低下抑制剤は、これを真水、海水、または塩水に添加して調製することで、次に説明する本発明の活牡蠣の保持用水溶液として使用することができる。また、収穫した活牡蠣を一時的に飼育している生け簀に、本発明の牡蠣の風味低下抑制剤を投入することで、牡蠣の風味低下抑制効果を得ることもできる。 By adding the oyster flavor reduction inhibitor of the present invention to fresh water, seawater, or salt water, it can be used as an aqueous solution for holding live oysters of the present invention, which will be described below. Further, by adding the oyster flavor reduction inhibitor of the present invention to the cage in which the harvested live oysters are temporarily bred, the oyster flavor reduction suppression effect can be obtained.

本発明の活牡蠣の保持用水溶液は、前記抽出物を含有するものである。該水溶液に活牡蠣を浸漬することで使用され、これによって、この使用後に活牡蠣を冷蔵保存または冷凍保存する際に牡蠣本来のミルキーで濃厚な風味が低下するのを抑制することができる。 The aqueous solution for holding live oysters of the present invention contains the extract. It is used by immersing live oysters in the aqueous solution, which can prevent the original milky and rich flavor of oysters from deteriorating when the live oysters are refrigerated or frozen after this use.

本発明の活牡蠣の保持用水溶液は、前記抽出物を固形分で0.1〜800ppm含有することが好ましい。当該含有量は、0.1〜240ppmがより好ましく、0.2〜240ppmが更に好ましく、0.2〜60ppmが特に好ましい。0.1ppmより少ないと牡蠣の風味低下抑制効果を発揮できない場合がある。800ppmを越えると前記抽出物に由来する色素成分が牡蠣に付着したり、牡蠣の風味が損なわれる場合がある。 The aqueous solution for holding live oysters of the present invention preferably contains the extract in a solid content of 0.1 to 800 ppm. The content is more preferably 0.1 to 240 ppm, further preferably 0.2 to 240 ppm, and particularly preferably 0.2 to 60 ppm. If it is less than 0.1 ppm, the effect of suppressing the deterioration of oyster flavor may not be exhibited. If it exceeds 800 ppm, the pigment component derived from the extract may adhere to the oyster or the flavor of the oyster may be impaired.

本発明の活牡蠣の保持用水溶液の塩化ナトリウム濃度は、2〜3.5重量%が好ましく、2.3〜3.5重量%がより好ましく、2.3〜3.2重量%がさらに好ましく、2.5〜3重量%が特に好ましい。塩化ナトリウム濃度が2重量%未満、又は3.5重量%を超えると、本発明の保持用水溶液中で牡蠣が生育できず、却って牡蠣の風味が損なわれる場合がある。 The sodium chloride concentration of the aqueous solution for holding live oysters of the present invention is preferably 2 to 3.5% by weight, more preferably 2.3 to 3.5% by weight, still more preferably 2.3 to 3.2% by weight. , 2.5 to 3% by weight is particularly preferable. If the sodium chloride concentration is less than 2% by weight or more than 3.5% by weight, oysters cannot grow in the holding aqueous solution of the present invention, and the flavor of oysters may be impaired.

本発明の活牡蠣の保持用水溶液は、上記塩化ナトリウム濃度を満足する限り、塩化ナトリウム以外の塩類を含有していてもよい。しかし、塩化ナトリウム以外の塩類の含有量は、塩化ナトリウム重量に対して1/3の重量までに制限することが好ましい。塩化ナトリウム以外の塩類の種類としては食品添加物として使用し得る塩類であれば特に限定されない。 The aqueous solution for holding live oysters of the present invention may contain salts other than sodium chloride as long as the above-mentioned sodium chloride concentration is satisfied. However, the content of salts other than sodium chloride is preferably limited to 1/3 by weight with respect to the weight of sodium chloride. The type of salts other than sodium chloride is not particularly limited as long as it is a salt that can be used as a food additive.

前記保持用水溶液は、上記塩化ナトリウム濃度を満足する限り、真水に食塩を添加して製造されたものであってもよいし、海水を利用して、又は、海水の塩化ナトリウム濃度を調整したものを利用して製造されたものであってもよい。 The holding aqueous solution may be produced by adding salt to fresh water as long as the sodium chloride concentration is satisfied, or using seawater or adjusting the sodium chloride concentration of seawater. It may be manufactured by using.

本発明の活牡蠣の保持用水溶液は、殺菌されたものであることが好ましい。具体的には、食品衛生法で定められた生食用かきの加工基準に基づいて、該水溶液の100ml当たり大腸菌群最確数100以下であることが好ましく、70以下であることがより好ましい。 The aqueous solution for holding live oysters of the present invention is preferably sterilized. Specifically, based on the processing standards for raw oysters defined by the Food Sanitation Law, the most probable number of coliform bacteria per 100 ml of the aqueous solution is preferably 100 or less, and more preferably 70 or less.

本発明の活牡蠣の保持用水溶液を用いて、特に生牡蠣を冷蔵または冷凍する前に、活牡蠣を処理することで、風味低下が抑制された牡蠣を製造することができる。当該処理の具体的態様の一例は以下のとおりである。前記水溶液を前記大腸菌群最確数になるよう殺菌した後、該水溶液の温度を、好適には1〜25℃の範囲とし、該水溶液に酸素を供給しつつ活牡蠣を浸漬して、好適には10〜15000分間保持する。その後、該水溶液から活牡蠣を取り出すことで、風味低下が抑制された牡蠣を得ることができる。水溶液の殺菌方法としては特に限定されず、例えば、紫外線照射による殺菌、オゾン殺菌、塩素殺菌などを行えばよい。 By treating the live oysters with the aqueous solution for holding the live oysters of the present invention, particularly before refrigerating or freezing the raw oysters, it is possible to produce oysters in which the deterioration of flavor is suppressed. An example of a specific mode of the process is as follows. After sterilizing the aqueous solution to the most probable number of coliform bacteria, the temperature of the aqueous solution is preferably set in the range of 1 to 25 ° C., and live oysters are immersed while supplying oxygen to the aqueous solution. Holds for 10 to 15,000 minutes. Then, by taking out the live oysters from the aqueous solution, it is possible to obtain oysters in which the deterioration of flavor is suppressed. The method for sterilizing the aqueous solution is not particularly limited, and for example, sterilization by ultraviolet irradiation, ozone sterilization, chlorine sterilization, or the like may be performed.

前記水溶液の温度は1〜25℃の範囲でコントロールすることが好ましく、1〜17℃がより好ましく、3〜17℃が更に好ましい。該温度が1℃より低い又は25℃より高いと、前記水溶液中で活牡蠣の活動が低下して牡蠣の風味低下抑制効果を発揮できない場合がある。 The temperature of the aqueous solution is preferably controlled in the range of 1 to 25 ° C, more preferably 1 to 17 ° C, still more preferably 3 to 17 ° C. If the temperature is lower than 1 ° C. or higher than 25 ° C., the activity of live oysters may be reduced in the aqueous solution, and the effect of suppressing the deterioration of oyster flavor may not be exhibited.

前記水溶液への酸素の供給方法としては、牡蠣の生育に必要な溶存酸素濃度を確保できれば、どのような方法であってもよい。 The method for supplying oxygen to the aqueous solution may be any method as long as the dissolved oxygen concentration required for the growth of oysters can be secured.

活牡蠣を前記水溶液中で保持する時間は、10〜15000分間が好ましく、15〜9000分間がより好ましく、20〜7200分間が更に好ましく、30〜6000分間が特に好ましく、150〜6000分間が極めて好ましい。保持時間が10分間より短いと風味低下抑制剤の活牡蠣に対する保持時間が足りず、牡蠣の風味低下抑制効果を発揮できない場合がある。15000分間より長いと牡蠣の風味が損なわれる場合がある。 The time for holding the live oyster in the aqueous solution is preferably 10 to 15000 minutes, more preferably 15 to 9000 minutes, further preferably 20 to 7200 minutes, particularly preferably 30 to 6000 minutes, and extremely preferably 150 to 6000 minutes. .. If the retention time is shorter than 10 minutes, the retention time of the flavor reduction inhibitor for live oysters may be insufficient, and the oyster flavor reduction suppression effect may not be exhibited. If it is longer than 15,000 minutes, the flavor of oysters may be impaired.

以上で説明した本発明の活牡蠣の保持用水溶液を用いた活牡蠣の処理は、静置した水槽で行ってもよいし、水槽ごと輸送しつつ行ってもよい。輸送しながら該処理をする場合であっても、本発明の方法によると、水溶液の温度が前述した範囲内にあれば厳格な温度管理を行う必要がないので、従来の魚介類を生きたまま輸送する方法と比較して輸送コストを抑制することができる。 The treatment of live oysters using the aqueous solution for holding live oysters of the present invention described above may be carried out in a stationary water tank or may be carried out while being transported together with the water tank. Even when the treatment is carried out while being transported, according to the method of the present invention, if the temperature of the aqueous solution is within the above-mentioned range, it is not necessary to perform strict temperature control, so that the conventional seafood remains alive. The transportation cost can be suppressed as compared with the transportation method.

本発明の牡蠣の製造方法に従って得られた牡蠣は、前述のように前記水溶液に特定時間保持した後、水揚げしてから殻むき、または、殻付きのまま冷蔵で1〜5日間保存しても、牡蠣本来のミルキーで濃厚な風味を維持している。また、本発明の牡蠣の製造方法に従って得られた牡蠣は、前述のように前記水溶液に特定時間保持した後、水揚げしてから、1ヶ月以上冷凍保存しても、牡蠣本来のミルキーで濃厚な風味を維持している。 The oysters obtained according to the method for producing oysters of the present invention may be kept in the aqueous solution for a specific time as described above, then landed and then peeled, or stored in a refrigerator with shells for 1 to 5 days. , Maintains the original milky and rich flavor of oysters. Further, the oysters obtained according to the method for producing oysters of the present invention are kept in the aqueous solution for a specific period of time as described above, and then landed and then frozen and stored for one month or more. Maintains flavor.

以下に実施例を示し、本発明をより具体的に説明するが、本発明はこれらの実施例に何ら限定されるものではない。なお、実施例において「部」や「%」は重量基準である。 Examples will be shown below and the present invention will be described in more detail, but the present invention is not limited to these examples. In the examples, "part" and "%" are based on weight.

(製造例1) エノキタケ抽出物の作製
市販のエノキタケ(子実体)100gに100℃に加熱した熱水200mlを加え、2時間保持した。その後固形分を濾別して、固形分量が0.6重量%のエノキタケ抽出物を得た。得られたエノキタケ抽出物の0.5重量%(W/W)固形分濃度の水溶液の粘度は17mPa・sであった。
(Production Example 1) Preparation of Enokitake Mushroom Extract 200 ml of hot water heated to 100 ° C. was added to 100 g of commercially available enokitake mushroom (fruiting body), and the mixture was kept for 2 hours. Then, the solid content was filtered off to obtain an enokitake extract having a solid content of 0.6% by weight. The viscosity of the obtained aqueous solution having a solid content concentration of 0.5% by weight (W / W) of the enokitake extract was 17 mPa · s.

(製造例2) 酵母抽出物の作製
パン酵母(株式会社カネカ製「カネカイーストGA」)100gに100℃に加熱した熱水200mlを加え、2時間保持した。その後固形分を濾別して、固形分量が1.0重量%の酵母抽出物を得た。得られた酵母抽出物の0.5重量%(W/W)固形分濃度の水溶液の粘度は8mPa・sであった。
(Production Example 2) Preparation of yeast extract 200 ml of hot water heated to 100 ° C. was added to 100 g of baker's yeast (“Kaneka East GA” manufactured by Kaneka Corporation), and the mixture was kept for 2 hours. Then, the solid content was filtered off to obtain a yeast extract having a solid content of 1.0% by weight. The viscosity of the aqueous solution of the obtained yeast extract having a solid content concentration of 0.5% by weight (W / W) was 8 mPa · s.

(製造例3) 大麦抽出物の作製
大麦粉(石橋工業株式会社製「大麦粉」)100gに100℃に加熱した熱水200mlを加え、2時間保持した。その後固形分を濾別して、固形分量が0.5重量%の大麦抽出物を得た。得られた大麦抽出物の0.5重量%(W/W)固形分濃度の水溶液の粘度は5mPa・sであった。
(Production Example 3) Preparation of barley extract 200 ml of hot water heated to 100 ° C. was added to 100 g of barley flour (“barley flour” manufactured by Ishibashi Kogyo Co., Ltd.) and kept for 2 hours. Then, the solid content was filtered off to obtain a barley extract having a solid content of 0.5% by weight. The viscosity of the aqueous solution of the obtained barley extract having a solid content concentration of 0.5% by weight (W / W) was 5 mPa · s.

(製造例4) 昆布抽出物の作製
真昆布(株式会社くらこん製「真昆布」)100gに100℃に加熱した熱水200mlを加え、2時間保持した。その後固形分を濾別して、固形分量が2.0重量%のコンブ抽出物を得た。得られたコンブ抽出物の0.5重量%(W/W)固形分濃度の水溶液の粘度は120mPa・sであった。
(Production Example 4) Preparation of kelp extract 200 ml of hot water heated to 100 ° C. was added to 100 g of kelp (“Makombu” manufactured by Kurakon Co., Ltd.) and kept for 2 hours. Then, the solid content was filtered off to obtain a kelp extract having a solid content of 2.0% by weight. The viscosity of the aqueous solution having a solid content concentration of 0.5% by weight (W / W) of the obtained kelp extract was 120 mPa · s.

<牡蠣のミルキーな風味評価>
実施例および比較例において得た牡蠣のむき身のミルキーな風味は、訓練された10名(男性5人、女性5人)のパネラーにより、以下の基準に基づき評価し、その平均値を評価値とした。
5点:実施例15の牡蠣よりも非常に良く、ミルキーな風味を非常に強く感じる
4点:実施例15の牡蠣よりも良く、ミルキーな風味を強く感じる
3点:実施例15の牡蠣と同等で、ミルキーな風味が感じられる
2点:実施例15の牡蠣よりも悪く、ミルキーな風味が感じられ難い
1点:実施例15の牡蠣よりも非常に悪く、ミルキーな風味が感じられない
<Evaluation of milky flavor of oysters>
The milky flavor of the peeled oysters obtained in the examples and comparative examples was evaluated by 10 trained panelists (5 males and 5 females) based on the following criteria, and the average value was used as the evaluation value. did.
5 points: Very better than the oysters of Example 15 and very strong milky flavor 4 points: Better than the oysters of Example 15 and strongly felt the milky flavor 3 points: Equivalent to the oysters of Example 15 And you can feel the milky flavor 2 points: It is worse than the oysters of Example 15 and it is hard to feel the milky flavor 1 point: It is much worse than the oysters of Example 15 and you can not feel the milky flavor

<牡蠣の濃厚な風味評価>
実施例および比較例において得た牡蠣のむき身の濃厚な風味は、訓練された10名(男性5人、女性5人)のパネラーにより、以下の基準に基づき評価し、その平均値を評価値とした。
5点:実施例15の牡蠣よりも非常に良く、濃厚な風味を非常に強く感じる
4点:実施例15の牡蠣よりも良く、濃厚な風味を強く感じる
3点:実施例15の牡蠣と同等で、濃厚な風味が感じられる
2点:実施例15の牡蠣よりも悪く、ややあっさりとしていて濃厚な風味が感じられ難い
1点:実施例15の牡蠣よりも非常に悪く、あっさりとしていて濃厚な風味が感じられない
<Evaluation of rich flavor of oysters>
The rich flavor of the peeled oysters obtained in the examples and comparative examples was evaluated by 10 trained panelists (5 males and 5 females) based on the following criteria, and the average value was used as the evaluation value. did.
5 points: Very better than the oysters of Example 15 and very strong flavor 4 points: Better than the oysters of Example 15 and strongly felt the rich flavor 3 points: Equivalent to the oysters of Example 15 And you can feel the rich flavor 2 points: worse than the oysters of Example 15 and slightly lighter and harder to feel the rich flavor 1 point: much worse than the oysters of Example 15 and light and rich I can't feel the flavor

<総合評価>
牡蠣のミルキーな風味及び牡蠣の濃厚な風味の各評価結果を基に、総合評価を行った。その際の評価基準は以下の通りである。
A:牡蠣のミルキーな風味、及び牡蠣の濃厚な風味が全て4.0点以上5.0点以下を満たすもの。
B:牡蠣のミルキーな風味、及び牡蠣の濃厚な風味が全て3.5点以上5.0点以下であって、且つ3.5以上4.0未満が少なくとも一つあるもの。
C:牡蠣のミルキーな風味、及び牡蠣の濃厚な風味が全て3.0点以上5.0点以下であって、且つ3.0以上3.5未満が少なくとも一つあるもの。
D:牡蠣のミルキーな風味、及び牡蠣の濃厚な風味が全て2.0点以上5.0点以下であって、且つ2.0以上3.0未満が少なくとも一つあるもの。
E:牡蠣のミルキーな風味、及び牡蠣の濃厚な風味の評価において、2.0未満が少なくとも一つあるもの。
<Comprehensive evaluation>
A comprehensive evaluation was performed based on the evaluation results of the milky flavor of oysters and the rich flavor of oysters. The evaluation criteria at that time are as follows.
A: The milky flavor of oysters and the rich flavor of oysters all satisfy 4.0 points or more and 5.0 points or less.
B: The milky flavor of oysters and the rich flavor of oysters are all 3.5 points or more and 5.0 points or less, and at least one of 3.5 points or more and less than 4.0 points.
C: The milky flavor of oysters and the rich flavor of oysters are all 3.0 points or more and 5.0 points or less, and at least one of 3.0 points or more and less than 3.5 points.
D: The milky flavor of oysters and the rich flavor of oysters are all 2.0 points or more and 5.0 points or less, and at least one of 2.0 or more and less than 3.0 points.
E: At least one of less than 2.0 in the evaluation of the milky flavor of oysters and the rich flavor of oysters.

(実施例1)
表1の配合に従い、製造例1で得られたエノキタケ抽出物1重量%と、食塩3重量%を配合した水溶液200リットルを水槽に満たし、水温を15℃にコントロールした。該水槽に、活牡蠣40体を浸漬し、酸素を供給しながら、180分間保持した後、牡蠣を水揚げした。水揚げ後、速やかに牡蠣の殻をむいて貝身を取り出し、牡蠣のむき身を得た。得られた牡蠣のむき身は、水気を切ってから真空包装し、4℃の冷蔵庫で24時間保存した後、牡蠣のミルキーな風味及び濃厚な風味を評価し、それらの結果を表1に示した。
(Example 1)
According to the formulation shown in Table 1, 200 liters of an aqueous solution containing 1% by weight of the enokitake extract obtained in Production Example 1 and 3% by weight of salt was filled in a water tank, and the water temperature was controlled to 15 ° C. Forty live oysters were immersed in the water tank, held for 180 minutes while supplying oxygen, and then the oysters were landed. Immediately after landing, the shells of the oysters were peeled and the shells were taken out to obtain the peeled oysters. The obtained peeled oysters were drained, vacuum-packed, stored in a refrigerator at 4 ° C. for 24 hours, and then the milky flavor and rich flavor of the oysters were evaluated, and the results are shown in Table 1. ..

Figure 2020045406
Figure 2020045406

(実施例2)
表1の配合に従い、前記エノキタケ抽出物の代わりに、製造例2で得られた酵母抽出物を配合した以外は、実施例1と同様にして牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表1に示した。
(Example 2)
Peeled oysters were obtained in the same manner as in Example 1 except that the yeast extract obtained in Production Example 2 was blended in place of the enokitake extract according to the formulation shown in Table 1. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 1.

(実施例3)
表1の配合に従い、前記エノキタケ抽出物の代わりに、製造例3で得られた大麦抽出物を配合した以外は、実施例1と同様にして牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表1に示した。
(Example 3)
Peeled oysters were obtained in the same manner as in Example 1 except that the barley extract obtained in Production Example 3 was blended in place of the enokitake extract according to the formulation shown in Table 1. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 1.

(比較例1)
表1の配合に従い、前記エノキタケ抽出物の代わりに、製造例4で得られた昆布抽出物を配合した以外は、実施例1と同様にして牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表1に示した。
(Comparative Example 1)
Peeled oysters were obtained in the same manner as in Example 1 except that the kelp extract obtained in Production Example 4 was blended in place of the enokitake extract according to the formulation shown in Table 1. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 1.

(比較例2)
この比較例は、特許文献1(特開2003−225047号公報)の実施例B−6に記載された鮮度保持剤に準拠したものである。表1の配合に従い、前記エノキタケ抽出物を配合せず、トレハロース、クエン酸、及び、ポリフェノールを配合した以外は、実施例1と同様にして牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表1に示した。
(Comparative Example 2)
This comparative example is based on the freshness-preserving agent described in Example B-6 of Patent Document 1 (Japanese Unexamined Patent Publication No. 2003-225847). According to the formulation shown in Table 1, oyster peeled meat was obtained in the same manner as in Example 1 except that the enokitake extract was not blended and trehalose, citric acid, and polyphenol were blended. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 1.

表1から明らかなように、抽出物の0.5%(W/W)固形分濃度の水溶液の粘度が3〜50mPa・sの範囲にあるエノキタケ抽出物(製造例1)、酵母抽出物(製造例2)、又は、大麦抽出物(製造例3)を含む食塩水で処理して得られた牡蠣のむき身(実施例1〜3)は、いずれもミルキーで濃厚な風味が感じられ、特にエノキタケ抽出物を含む食塩水で処理して得られたもの(実施例1)の風味が極めて良好であった。一方、抽出物の0.5%(W/W)固形分濃度の水溶液の粘度が120mPa・sの昆布抽出物(製造例4)を含む食塩水で処理して得られた牡蠣のむき身(比較例1)は、ミルキーな風味と濃厚な風味が共に弱く感じられた。また、トレハロース、クエン酸、及び、ポリフェノールを含む食塩水で処理して得られた牡蠣のむき身(比較例2)は、濃厚な風味は若干感じられたものの、ミルキーな風味は弱く、しかもポリフェノールによる着色が見られた。 As is clear from Table 1, the enokitake extract (Production Example 1) and the yeast extract (Production Example 1) in which the viscosity of the aqueous solution having a solid content concentration of 0.5% (W / W) of the extract is in the range of 3 to 50 mPa · s. The peeled oysters (Examples 1 to 3) obtained by treating with a saline solution containing a barley extract (Production Example 3) or Production Example 2) have a milky and rich flavor, and in particular. The flavor of the product obtained by treating with a saline solution containing the Enokitake extract (Example 1) was extremely good. On the other hand, peeled oysters obtained by treating with a saline solution containing a kelp extract (Production Example 4) having a viscosity of an aqueous solution having a solid content of 0.5% (W / W) of the extract of 120 mPa · s (comparison). In Example 1), both the milky flavor and the rich flavor were felt weak. In addition, the oyster peeled meat (Comparative Example 2) obtained by treating with a saline solution containing trehalose, citric acid, and polyphenols had a slightly rich flavor, but a weak milky flavor, and was due to polyphenols. Coloring was seen.

(実施例4〜7、比較例3)
表2の配合に従い、エノキタケ抽出物の配合量を1.0重量%から、0.002重量%(実施例4)、0.1重量%(実施例5)、4.0重量%(実施例6)、13.0重量%(実施例7)、又は、15.0重量%(比較例3)に変更した以外は、実施例1と同様にして牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表2に示した。
(Examples 4 to 7, Comparative Example 3)
According to the formulation in Table 2, the blending amount of the Enokitake extract was changed from 1.0% by weight to 0.002% by weight (Example 4), 0.1% by weight (Example 5), 4.0% by weight (Example). 6) Peeled oysters were obtained in the same manner as in Example 1 except that the content was changed to 13.0% by weight (Example 7) or 15.0% by weight (Comparative Example 3). The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 2.

Figure 2020045406
Figure 2020045406

(比較例4)
表2の配合に従い、エノキタケ抽出物を配合せずに調製した水溶液を使用した以外は、実施例1と同様にして牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表2に示した。
(Comparative Example 4)
Peeled oysters were obtained in the same manner as in Example 1 except that an aqueous solution prepared without adding the enokitake extract was used according to the formulation shown in Table 2. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 2.

表2から明らかなように、水溶液中のエノキタケ抽出物の固形分量が0.1〜800ppmの範囲にある食塩水で処理して得られた牡蠣のむき身(実施例1、4〜7)は、いずれもミルキーで濃厚な風味が感じられ、特に前記固形分量が60ppmの食塩水で処理して得られたもの(実施例1)の風味は極めて良好であった。一方、エノキタケ抽出物の固形分量が900ppmの食塩水で処理して得られた牡蠣のむき身(比較例3)は、濃厚な風味が若干感じられたものの、ミルキーな風味は弱かった。また、エノキタケ抽出物を含有しない食塩水で処理して得られた牡蠣のむき身(比較例4)は、ミルキーな風味と濃厚な風味が共に弱く感じられた。 As is clear from Table 2, the peeled oysters (Examples 1, 4 to 7) obtained by treating with a saline solution in which the solid content of the enokitake extract in the aqueous solution is in the range of 0.1 to 800 ppm are obtained. In each case, a milky and rich flavor was felt, and in particular, the flavor obtained by treating with a saline solution having a solid content of 60 ppm (Example 1) was extremely good. On the other hand, the peeled oysters (Comparative Example 3) obtained by treating the enokitake extract with a saline solution having a solid content of 900 ppm had a slightly rich flavor, but a weak milky flavor. In addition, the peeled oysters (Comparative Example 4) obtained by treating with a saline solution containing no enokitake extract had a weak milky flavor and a rich flavor.

(実施例8〜10)
表3の条件に従い、食塩の配合量を3.0重量%から、2.0重量%(実施例8)、2.3重量%(実施例9)、又は、3.5重量%(実施例10)に変更した以外は、実施例1と同様にして牡蠣を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表3に示した。
(実施例11)
表3の条件に従い、塩を含む水溶液の種類を食塩水から海水へ変更した以外は、実施例1と同様にして牡蠣を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表3に示した。
(Examples 8 to 10)
According to the conditions in Table 3, the amount of salt blended was changed from 3.0% by weight to 2.0% by weight (Example 8), 2.3% by weight (Example 9), or 3.5% by weight (Example). Oysters were obtained in the same manner as in Example 1 except that they were changed to 10). The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 3.
(Example 11)
Oysters were obtained in the same manner as in Example 1 except that the type of the aqueous solution containing salt was changed from saline solution to seawater according to the conditions shown in Table 3. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 3.

Figure 2020045406
Figure 2020045406

(比較例5)
この比較例は、特許文献2(特開2010−154799号公報)の実施例1−1に記載された貝類の品質改良剤に準拠したものである。表3の配合に従い、水溶液の塩の種類を食塩から乳酸ナトリウムと炭酸水素ナトリウムへ変えた以外は、実施例1と同様にして牡蠣を得た。
(Comparative Example 5)
This comparative example is based on the shellfish quality improving agent described in Example 1-1 of Patent Document 2 (Japanese Unexamined Patent Publication No. 2010-154799). Oysters were obtained in the same manner as in Example 1 except that the type of salt in the aqueous solution was changed from sodium lactate to sodium hydrogen carbonate according to the formulation shown in Table 3.

表3から明らかなように、エノキタケ抽出物を含み塩化ナトリウム濃度が2〜3.5重量%の範囲にある水溶液で処理して得られた牡蠣のむき身(実施例1、8〜11)は、いずれもミルキーで濃厚な風味が強く感じられた。一方、エノキタケ抽出物を含有するが塩化ナトリウムの代わりに乳酸ナトリウムと炭酸水素ナトリウムの合計濃度を3.5重量%とした水溶液に浸漬した活牡蠣(比較例5)は、180分間の浸漬によって死滅し、ミルキーな風味と濃厚な風味が共にあまり感じられなくなった。 As is clear from Table 3, the peeled oysters (Examples 1, 8 to 11) obtained by treating with an aqueous solution containing an enokitake extract and having a sodium chloride concentration in the range of 2 to 3.5% by weight were obtained. All of them had a strong milky and rich flavor. On the other hand, live oysters (Comparative Example 5) containing an enokitake extract but immersed in an aqueous solution containing 3.5% by weight of sodium lactate and sodium hydrogen carbonate instead of sodium chloride were killed by immersion for 180 minutes. However, both the milky flavor and the rich flavor became less noticeable.

(実施例12〜14)
表4の条件に従い、水溶液の水温を15℃から、2℃(実施例12)、又は、10℃(実施例13)、又は、22℃(実施例14)にそれぞれ変更した以外は、実施例1と同様にして牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表4に示した。
(Examples 12 to 14)
Examples except that the water temperature of the aqueous solution was changed from 15 ° C. to 2 ° C. (Example 12), 10 ° C. (Example 13), or 22 ° C. (Example 14) according to the conditions shown in Table 4. The peeled oysters were obtained in the same manner as in 1. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 4.

Figure 2020045406
Figure 2020045406

表4から明らかなように、エノキタケ抽出物を含み水温が1〜25℃の食塩水で処理して得られた牡蠣のむき身(実施例1、12〜14)は、いずれもミルキーで濃厚な風味が感じられ、特にエノキタケ抽出物を含み水温が15℃又は10℃の食塩水で処理して得られたもの(実施例1及び13)の風味は極めて良好であった。 As is clear from Table 4, the peeled oysters (Examples 1, 12 to 14) obtained by treating with a saline solution containing the enokitake extract and having a water temperature of 1 to 25 ° C. are all milky and have a rich flavor. In particular, the flavors of those obtained by treating with a saline solution containing an enokitake extract and having a water temperature of 15 ° C. or 10 ° C. (Examples 1 and 13) were extremely good.

(実施例15〜17、比較例6及び7)
表5の条件に従い、水溶液中での牡蠣の保持時間を180分間から、15分間(実施例15)、8000分間(実施例16)、14800分間(実施例17)、5分間(比較例6)、又は、17280分間(比較例7)に変更した以外は、実施例1と同様にして牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表5に示した。
(Examples 15 to 17, Comparative Examples 6 and 7)
According to the conditions in Table 5, the retention time of oysters in the aqueous solution was changed from 180 minutes to 15 minutes (Example 15), 8000 minutes (Example 16), 14800 minutes (Example 17), 5 minutes (Comparative Example 6). Or, the peeled oysters were obtained in the same manner as in Example 1 except that the time was changed to 17280 minutes (Comparative Example 7). The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 5.

Figure 2020045406
Figure 2020045406

表5から明らかなように、エノキタケ抽出物を含む食塩水に10〜15000分間保持して得られた牡蠣のむき身(実施例1、15〜17)は、いずれもミルキーで濃厚な風味が感じられ、特に保持時間を180分間とした牡蠣のむき身(実施例1)の風味は極めて良好であった。一方、保持時間が5分間の牡蠣のむき身(比較例6)は、ミルキーな風味と濃厚な風味が共に弱く感じられた。また、保持時間が17280分間の牡蠣のむき身(比較例7)は、濃厚な風味が若干感じられたものの、ミルキーな風味は弱かった。 As is clear from Table 5, the peeled oysters (Examples 1, 15 to 17) obtained by holding the oysters in a saline solution containing the enokitake extract for 10 to 15,000 minutes all had a milky and rich flavor. In particular, the flavor of the peeled oyster meat (Example 1) having a holding time of 180 minutes was extremely good. On the other hand, the peeled oyster meat (Comparative Example 6) having a holding time of 5 minutes was felt to have a weak milky flavor and a rich flavor. In addition, the peeled oyster meat (Comparative Example 7) having a holding time of 17280 minutes had a slightly rich flavor, but a weak milky flavor.

(実施例18、19)
表6の条件に従い、冷蔵庫でのむき身の保存時間を24時間から、96時間(実施例18)、又は、120時間(実施例19)に変更した以外は実施例1と同様にして、牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表6に示した。
(Examples 18 and 19)
Oysters were stored in the refrigerator in the same manner as in Example 1 except that the storage time of the peeled meat in the refrigerator was changed from 24 hours to 96 hours (Example 18) or 120 hours (Example 19). I got a peeled meat. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 6.

Figure 2020045406
Figure 2020045406

(実施例20)
表6の条件に従い、実施例1と同様に活牡蠣を水槽中に保持した後、むき身にせず、殻付きのまま冷蔵庫で96時間保存した以外は実施例18と同様にして、殻付き牡蠣を得た。その後、牡蠣の殻をむいて貝身を取り出して、ミルキーな風味及び濃厚な風味を評価し、それらの結果を表6に示した。
(Example 20)
According to the conditions of Table 6, after holding the live oysters in the aquarium in the same manner as in Example 1, the oysters with shells were prepared in the same manner as in Example 18 except that the oysters were stored in the refrigerator with shells for 96 hours without being peeled. Obtained. Then, the oysters were peeled and the shellfish was taken out to evaluate the milky flavor and the rich flavor, and the results are shown in Table 6.

(実施例21)
表6の条件に従い、実施例1と同様にして牡蠣のむき身を得た後、水気を切ってから真空包装し、−20℃の冷凍庫で1ヶ月保存し、その後4℃の冷蔵庫で24時間保存して解凍し、牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表6に示した。
(Example 21)
According to the conditions in Table 6, after obtaining the peeled oysters in the same manner as in Example 1, drain the oysters, vacuum-pack them, store them in a freezer at -20 ° C for 1 month, and then store them in a refrigerator at 4 ° C for 24 hours. And thawed to get the peeled oysters. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 6.

(実施例22)
表6の条件に従い、冷蔵庫でのむき身の保存時間を24時間から、0時間に変更した(すなわち冷蔵保存を行わなかった)以外は実施例1と同様にして、牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表6に示した。
(Example 22)
According to the conditions of Table 6, the peeled oysters were obtained in the same manner as in Example 1 except that the storage time of the peeled meat in the refrigerator was changed from 24 hours to 0 hours (that is, the refrigerated storage was not performed). The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 6.

(比較例8)
表6の条件に従い、エノキタケ抽出物を配合せずに調製した水溶液を使用した以外は、実施例19と同様にして牡蠣のむき身を得た。得られた牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表6に示した。
(Comparative Example 8)
Peeled oysters were obtained in the same manner as in Example 19 except that an aqueous solution prepared without adding the enokitake extract was used according to the conditions shown in Table 6. The milky flavor and rich flavor of the obtained peeled oysters were evaluated, and the results are shown in Table 6.

(参考例)
水溶液中での活牡蠣の保持を行うことなく、活牡蠣の殻をむいて貝身を取り出して牡蠣のむき身を得た後、冷蔵保存も行わずに、牡蠣のむき身のミルキーな風味及び濃厚な風味を評価し、それらの結果を表6に示した。
(Reference example)
Without retaining the live oysters in the aqueous solution, the shells of the live oysters are peeled off to obtain the peeled oysters, and then the peeled oysters have a milky flavor and richness without refrigeration. The flavor was evaluated and the results are shown in Table 6.

表6から明らかなように、エノキタケ抽出物を含む食塩水で処理した後、むき身で又は殻付きのまま0時間〜120時間冷蔵保存して得られた牡蠣(実施例1、18、19、20及び22)はいずれもミルキーで濃厚な風味が感じられた。エノキタケ抽出物を含む食塩水で処理した後1ヶ月間冷凍保存して得られた牡蠣のむき身(実施例21)もミルキーで濃厚な風味が感じられた。特に、水溶液から水揚げ後即座に殻むきして冷蔵保存せずに得られた牡蠣のむき身(実施例22)、及び、水溶液から水揚げ後殻むきし24時間冷蔵保存して得られた牡蠣のむき身(実施例1)の風味は、水溶液での保持も冷蔵保存も行っていない牡蠣のむき身(参考例)と同レベルで、極めて良好であった。一方、エノキタケ抽出物を含有しない食塩水で処理して得られた牡蠣のむき身を120時間冷蔵保存した牡蠣のむき身(比較例8)は、24時間冷蔵保存したもの(比較例4)と同様、ミルキーな風味と濃厚な風味が共に弱く感じられた。
As is clear from Table 6, oysters obtained by treating with a saline solution containing an enokitake extract and then refrigerating the oysters with peeled meat or with shells for 0 to 120 hours (Examples 1, 18, 19, 20). In both cases and 22), a milky and rich flavor was felt. The peeled oysters (Example 21) obtained by freezing for one month after treatment with a saline solution containing the enokitake extract also had a milky and rich flavor. In particular, the peeled oysters obtained by immediately peeling the shells from the aqueous solution without refrigerating (Example 22) and the peeled oysters obtained by refrigerating the shells after landing from the aqueous solution for 24 hours. The flavor of (Example 1) was extremely good, at the same level as the peeled oysters (reference example) that were neither retained in an aqueous solution nor refrigerated. On the other hand, the oyster peels obtained by treating with a saline solution containing no enokitake extract and refrigerated for 120 hours (Comparative Example 8) are the same as those obtained by refrigerating for 24 hours (Comparative Example 4). Both the milky flavor and the rich flavor were felt weak.

Claims (7)

エノキタケ抽出物、酵母抽出物及び大麦抽出物からなる群より選ばれる少なくとも1種の抽出物を含む、牡蠣の風味低下抑制剤。 An oyster flavor reduction inhibitor containing at least one extract selected from the group consisting of enokitake extract, yeast extract and barley extract. 前記抽出物を、前記牡蠣の風味低下抑制剤の乾燥重量全体中、乾燥重量で50〜100重量%含む、請求項1に記載の牡蠣の風味低下抑制剤。 The oyster flavor reduction inhibitor according to claim 1, wherein the extract is contained in an amount of 50 to 100% by weight based on the dry weight of the oyster flavor reduction inhibitor. エノキタケ抽出物、酵母抽出物及び大麦抽出物からなる群より選ばれる少なくとも1種の抽出物を固形分で0.1〜800ppm含有し、塩化ナトリウム濃度が2〜3.5重量%である、活牡蠣の保持用水溶液。 At least one extract selected from the group consisting of enokitake extract, yeast extract and barley extract is contained in a solid content of 0.1 to 800 ppm and has a sodium chloride concentration of 2 to 3.5% by weight. An aqueous solution for holding oysters. 請求項1又は2に記載の牡蠣の風味低下抑制剤を用いた請求項3に記載の水溶液。 The aqueous solution according to claim 3, which uses the oyster flavor reduction inhibitor according to claim 1 or 2. 温度が1〜25℃の範囲にある請求項3又は4に記載の水溶液中に、酸素が供給された状態で10〜15000分間保持された活牡蠣。 Live oysters kept in an aqueous solution according to claim 3 or 4 having a temperature in the range of 1 to 25 ° C. for 10 to 15,000 minutes while being supplied with oxygen. 請求項5に記載の活牡蠣が冷蔵または冷凍された生牡蠣。 Raw oysters in which the live oysters according to claim 5 are refrigerated or frozen. エノキタケ抽出物、酵母抽出物及び大麦抽出物からなる群より選ばれる少なくとも1種の抽出物を固形分で0.1〜800ppm含有し、塩化ナトリウム濃度が2〜3.5重量%、温度が1〜25℃の範囲にある水溶液中に、酸素が供給された状態で活牡蠣を10〜15000分間保持することを特徴とする、風味低下が抑制された牡蠣の製造方法。 At least one extract selected from the group consisting of Enokitake extract, yeast extract and barley extract is contained in a solid content of 0.1 to 800 ppm, a sodium chloride concentration of 2 to 3.5% by weight, and a temperature of 1. A method for producing an oyster with suppressed flavor deterioration, which comprises holding a live oyster for 10 to 15,000 minutes in a state where oxygen is supplied in an aqueous solution in the range of ~ 25 ° C.
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