JPWO2019078070A1 - Laminate - Google Patents

Abstract

In view of the background of the prior art, the present invention is to provide a laminate suitable for a biosensor in which the amount of a liquid sample used is reduced and the manufacturing process is simplified. A laminate having a cover material B, a resin layer X, a base material A, a resin layer Y, and a cover material C in this order, having a cavity having an introduction port in the laminate, and the cavity having a specific surface. A laminate characterized by being composed of.

Description

The present invention relates to a laminate, and in particular, inspects and quantifies specific components contained in a biological fluid such as blood, urine, saliva, interstitial fluid, etc., in various trace amounts of liquid samples. It relates to a laminate suitable for a biosensor used in the process. By providing a cavity into which the liquid sample is introduced, the laminate of the present invention can reduce the amount of the liquid sample used and can simplify the manufacturing process.

Biosensors are used to test and quantify specific components in various trace amounts of liquid samples, such as blood, urine, saliva, interstitial fluid, and other biological fluids. Biosensors of various configurations have been proposed, but each configuration includes a cavity into which a liquid sample is introduced. This cavity is composed of two members generally called a spacer and a cover, and is a base material A (hereinafter, may be referred to as a spacer base material) and a cover material B or a cover material C (hereinafter, a cover material B) used for the spacer. Alternatively, each of the cover materials C, or both of them may be collectively referred to as a cover material), may be formed by laminating them via a resin layer.
In biosensors, reducing the amount of biological fluids used as liquid samples, such as blood, urine, saliva, and interstitial fluid, has been emphasized as a method for reducing the burden on patients when using the sensor. This has been done by reducing the volume of the cavity.
For example, there is known a configuration in which adhesive layers are provided on both sides of a spacer base material, one side is bonded with a hydrophilic cover, and the other side is bonded with a base material having electrodes (see Patent Document 1). ). However, in this method, not only the thickness of the spacer base material but also the thickness of the adhesive layers formed on both sides of the spacer base material increases the volume of the cavity, which is a heavy burden on the patient.

Japanese Patent Application Laid-Open No. 2002-214187

In view of the background of the prior art, the present invention is to provide a laminate suitable for a biosensor in which the amount of a liquid sample used is reduced and the manufacturing process is simplified.

The present invention employs the following means in order to solve such a problem.
(1) A laminate having a cover material B, a resin layer X, a base material A, a resin layer Y, and a cover material C in this order, wherein the laminate has a cavity having an introduction port, and the cavity has a cavity. A laminate characterized by being composed of the following faces (i), (ii), and (iii).
(I) One surface composed of the resin layer X (ii) One surface composed of the resin layer Y (iii) Three surfaces composed of only the base material A (2) Cover material B, resin layer X, base material A laminate having A, a resin layer Y, and a cover material C in this order, the laminate having a cavity having an introduction port, and the cavity having the following surfaces (iv) and (v). A laminate characterized by being composed of (vi) planes.
(Iv) One surface composed of the resin layer X (v) Three surfaces composed of only the base material A and the resin layer Y (vi) One surface composed of the cover material C (3) The resin layer X or the resin The laminate according to (1) or (2), wherein the water contact angle on the surface of at least one of the resin layers of the layer Y is less than 15 degrees.
(4) Described in any one of (1) to (3), wherein the adhesive strength between the base material A and the resin layer Y at a peeling angle of 90 ° in accordance with JIS Z0237: 2009 is 2.5 N / 10 mm or more. Laminated body.
(5) The laminate according to any one of (1) to (4), wherein the resin layer X and / or the resin layer Y contains a surfactant.
(6) A group in which the resin layer X and / or the resin layer Y is composed of a polyester resin, a (meth) acrylic resin, a polyolefin resin, an ethylene-vinyl acetate copolymer resin, a polyamide resin, a chloroprene resin, an aramid resin, and an acrylic urethane resin. The laminate according to any one of (1) to (5), which contains at least one resin selected from the above.
(7) The laminate according to (5) or (6), wherein the surfactant is a nonionic surfactant having a polyalkylene glycol skeleton.

According to the present invention, in view of the background of the prior art, the spacer thickness is reduced by arranging the resin layer which was conventionally arranged as a spacer as a cover, which is required when introducing a liquid sample. It is possible to reduce the amount of liquid sample. Further, in the conventional configuration shown in FIG. 6, the layer imparted with hydrophilicity is provided independently, but by imparting hydrophilicity to the resin layer, the layer becomes one layer having both hydrophilicity and adhesiveness at the same time. Since it can be integrated, the manufacturing process can be simplified.
Further, since the present invention has a structure in which the resin layers are provided on both sides of the base material A, the contact area between the liquid sample and the resin layer is increased as compared with the conventional case. Therefore, especially when the resin layer is hydrophilic, the introduction speed of the liquid sample can be improved.

FIG. 1 is a diagram showing the configuration of laminated bodies 1 and 2 in Examples 1 and 2. FIG. 2 is a perspective view of the laminated body in FIG. FIG. 3 is a diagram showing a configuration corresponding to the laminated bodies 3 to 18 in Examples 3 to 18. FIG. 4 is a perspective view of the laminated body in FIG. FIG. 5 is a conceptual diagram of the cavity. FIG. 6 is a conventional configuration diagram (a diagram showing the configuration of the laminated body 19 in Comparative Example 1).

Hereinafter, the present invention will be described in detail together with preferred embodiments. The present invention relates to a laminate suitable for a biosensor, and is a laminate having a cover material B, a resin layer X, a base material A, a resin layer Y, and a cover material C in this order, and is contained in the laminate. It is a laminated body having a cavity provided with an introduction port, and the cavity is composed of a specific surface.

Further, in the present invention, the surface constituting the cavity is composed of only (i) one surface composed of the resin layer X, (ii) one surface composed of the resin layer Y, and (iii) the base material A. The aspect composed of three surfaces is referred to as Invention 1, and the surface constituting the cavity is composed of only one surface composed of (iv) resin layer X, (v) base material A, and resin layer Y. An aspect composed of three surfaces and one surface composed of (vi) cover material C is referred to as the present invention 2, and the present invention 1 and the present invention 2 are collectively referred to as the present invention.
A biosensor is a sensor that converts a biological reaction into an electric signal by using an enzyme, a microorganism, or the like. Further, the biosensor has a cavity, has a structure similar to a laminated body or a laminated body that sucks a liquid sample from the inlet of the cavity, and is used by inserting it into the measuring instrument main body. Due to its shape, the biosensor is also commonly referred to as a sensor chip or chip. By using the biosensor and the measuring instrument body in combination, it is possible to quantify the substance to be measured contained in the liquid sample introduced into the cavity. Sensors using enzymes are being put to practical use, and are being developed and used especially for medical and health management applications.

The thickness of the base material A used in the present invention is not particularly limited, but is preferably 500 μm or less because it is preferable to have flexibility from the viewpoint of improving the workability of assembling the laminated body, and the strength against tension and impact is ensured. From the viewpoint, 10 μm or more is preferable. Further, when a plastic film is used as the base material A, the thickness of the base material A is preferably 20 μm to 300 μm, more preferably 30 μm to 250 μm, from the viewpoint of ease of processing and handling of the film. Further, since the thickness of the base material A is one parameter that defines the volume of the cavity into which the liquid sample is introduced, it is more preferably thinner than 200 μm from the viewpoint of reducing the amount of the liquid sample used.

The cavity is provided by completely penetrating at least the base material A in the thickness direction, and is represented by reference numeral 4 in FIGS. 1 to 4. Further, the cavity may not only penetrate the base material A in the thickness direction but also penetrate the resin layer Y in the thickness direction. For example, the cavity that penetrates the base material A and the resin layer Y in the thickness direction is represented by reference numeral 4 in FIGS. 3 and 4. As shown in FIG. 5, the cavity in the present invention is provided with an introduction port (reference numeral 41) for introducing a liquid sample, and the cavity 4 is further composed of a plurality of surfaces. As shown in FIGS. 2, 4 and 5, the cavity 4 has an opening on the introduction port side, and a liquid sample can be introduced into the cavity 4 from the introduction port. Further, the opposite side of the inlet of the cavity is not an opening but a surface constituting the cavity. As a result, it is possible to prevent the liquid sample introduced from the introduction port from overflowing from the opposite side of the introduction port, and further, the liquid sample is located at the tip of the cavity (the inner part of the cavity, that is, the position far from the introduction port of the cavity). A reagent layer or the like for detecting the composition of the above can be provided. It should be noted that FIGS. 1 and 3 are cross-sectional views of the laminated body cut in the thickness direction between the introduction port and the opposite side of the introduction port (that is, the surface facing the introduction port) and viewed from the introduction port side. ..

Further, the base material A preferably has appropriate processability in order to form a cavity. The cavity does not include, for example, a groove provided by scraping the surface of the base material A (that is, a groove that does not penetrate the base material A in the thickness direction). The method of forming the cavity is not particularly limited, but physical processing may be performed by cutting with scissors or a cutter knife, or by punching using a mold, pinnacle mold, Thomson mold, engraving mold, or the like. Alternatively, a method using known energy rays typified by laser processing may be used. The cavity may be provided for a configuration in which three layers of the base material A, the resin layer and the silicone-based resin film described later are laminated, and the cavity is provided from either the base material A side or the silicone-based resin film side. May be good.

The forms of the base material A, the cover material B, and the cover material C used in the present invention are not particularly limited, but for example, a plastic film, synthetic paper, paper, or a composite sheet with surface treatment is preferable, and dimensional stability is particularly preferable. A plastic film is preferable from the viewpoint of durability and durability.
Materials for plastic films include polyester, polyolefin, polyamide, polyesteramide, polyether, polyimide, polyamideimide, polystyrene, polycarbonate, poly-ρ-phenylene sulfide, polyether ester, polyvinyl chloride, and poly (meth) acrylic acid ester. Can be mentioned. Further, these copolymers, blends and further crosslinked compounds can also be used.
Further, among the above plastic films, polyesters include, for example, polyethylene terephthalate, polyethylene 2,6-naphthalate, polyethylene α, β-bis (2-chlorophenoxy) ethane 4,4'-dicarboxylate, polybutylene terephthalate and the like. A film is preferable, and among these, a film made of polyethylene terephthalate is particularly preferably used in consideration of mechanical properties, quality such as workability, and economic efficiency.

The base material A used in the present invention is preferably a single layer, but for example, a layer having a thickness of 1 μm or less (for example, for the purpose of improving the adhesive strength with the adjacent resin layer X and / or the resin layer Y). An easy-adhesion layer) may be provided on the surface layer to form a laminated structure.
A layer different from the resin layers X and Y may be provided between the cover material B and the resin layer X adjacent thereto and / or between the cover material C and the resin layer Y adjacent thereto, if necessary. .. By further performing surface treatment such as corona discharge treatment and primer treatment on the surfaces of different layers, the resin layers X and Y to be laminated on the different layers can be formed more firmly. Alternatively, carbon or gold, between the cover material B and the adjacent resin layer X and / or between the cover material C and the adjacent resin layer Y, to function as an electrode material used in the biosensor. An electrode layer made of a noble metal such as platinum or palladium may be provided by means such as various printing methods such as screen printing or a sputtering vapor deposition method. It is preferable to provide a measurement electrode, a counter electrode, and a detection electrode in the electrode layer provided between the cover material B and the resin layer X adjacent thereto and / or between the cover material C and the resin layer Y adjacent thereto. .. When the laminate of the present invention is used as a biosensor, a reagent layer containing an enzyme, a mediator, or the like that specifically reacts with a specific component in a liquid sample may be formed on the electrode layer.

The laminate of the present invention is suitably used as a biosensor. Therefore, the biosensor having the laminate of the present invention has a reagent layer and an electrode layer in addition to the laminate.
A method of using a biosensor is shown by taking blood glucose measurement as an example. When the biosensor having the above configuration is inserted into the main body of the measuring device and then the blood is brought into contact with the opening of the cavity of the biosensor, the blood is sucked into the cavity and the blood is contained in the reagent layer arranged at the tip of the cavity. An electric current is generated by reacting with the enzyme. This current corresponds to the blood glucose level in the blood, is transmitted to the measuring device main body via the mediator and the electrode layer, and is converted into the blood glucose level by the arithmetic unit incorporated in the measuring device main body.

Further, the laminate of the present invention is a laminate having a cover material B and a cover material C on each surface of the base material A via resin layers X and Y, respectively, and the cover material C is a cavity together with the base material A. May constitute a surface of.
Further, as shown in FIG. 1, the cover film 5 can be composed of two layers, that is, the cover material B21 and the resin layer X31, or the cover material C22 and the resin layer Y32. The laminate 6 of FIG. 1 is a laminate 6 having a cover material B21 and a cover material C22 on each surface of the base material A1 via a resin layer X31 and a resin layer Y32, respectively, and the resin layer X31 together with the base material A1. And the resin layer Y32 constitutes the surface of the cavity 4.
The surfaces 7a to 10b of the cavity into which the liquid sample is introduced are preferably hydrophilic. Hydrophilicity is a measure of the wettability and spread of water when water is dropped on the surface. If the hydrophilicity is high, the effect of lowering the surface tension of the liquid sample is synergistic in addition to the normal capillary phenomenon, and the introduction time. Can be shortened. Therefore, in the laminated body configuration, the resin layer forming the surface of the cavity (for example, the resin layer represented by reference numerals 7a and 7b in FIGS. 1 and 3 and reference numerals 10a and 10b in FIG. 3) will be described later. High hydrophilicity can be imparted by the method described above. Further, as the area ratio of the hydrophilic surface among the five surfaces constituting the cavity is larger, the introduction speed of the liquid sample into the cavity is improved. Therefore, both sides of the base material A are the resin having the hydrophilicity. It is more preferable to be composed of layers. That is, it is preferable that all or a part of at least one surface of the cavity is formed of the resin layer X or the resin layer Y. Here, "all or part of at least one surface of the cavity is formed by the resin layer X or the resin layer Y" means, for example, among the five surfaces of the cavity as shown by reference numeral 7a in FIG. When at least one surface is formed of the resin layer (a mode in which the entire at least one surface of the cavity is formed of the resin layer X or the resin layer Y), for example, it is indicated by reference numerals 10a and 10b in FIG. Such a case where at least one surface of the cavity is formed of the base material A and the resin layer Y (a mode in which a part of at least one surface of the cavity is formed of the resin layer X or the resin layer Y). ..

As described above, the cavity in the present invention is provided with an introduction port for introducing a liquid sample, and the surface constituting the cavity is composed of (i) one surface composed of the resin layer X and (ii) the resin layer Y. One surface to be formed, three surfaces composed of only (iii) base material A, or one surface composed of (iv) resin layer X, (v) only base material A and resin layer Y It is composed of three surfaces composed of (vi) and one surface composed of the cover material C. Since the cavity is composed of a plurality of types of surfaces, it becomes easy to arbitrarily adjust the hydrophilicity by adjusting the composition of each surface. Further, the surface forming the cavity does not necessarily have to be a flat surface, and for example, a curved surface having a curved surface or a surface having an unevenness is also included in the surface forming the cavity.
Here, the "one surface composed of the resin layer X" in (i) and (iv) means that one surface constituting the cavity is composed of the resin layer X as shown by reference numeral 7a in FIGS. 1 and 3. It means to be done. Similarly, the “one surface composed of the resin layer Y” in (ii) means that one surface constituting the cavity is composed of the resin layer Y, as shown by reference numeral 7b in FIG.

The “three surfaces composed of only the base material A” in (iii) means that the three surfaces constituting the cavity are composed of only the base material A, as shown by reference numerals 8a and 8b in FIG. .. At this time, for example, the surfaces composed of the base material A, the resin layer X, and the resin layer Y as shown by reference numerals 12a and 12b in FIG. 6 do not correspond to "three surfaces composed of only the base material A". And.
The “three surfaces composed of only the base material A and the resin layer Y” in (v) means that the three surfaces constituting the cavity are the base material A and the resin layer Y, as shown by reference numerals 10a and 10b in FIG. It means that it is composed only of. At this time, for example, in a configuration in which another layer is included between the base material A and the resin layer Y, the three surfaces forming the cavity include the other layer in addition to the base material A and the resin layer Y. Therefore, it does not correspond to "three surfaces composed of only the base material A and the resin layer Y".

The “one surface made of the cover material C” in (vi) means that one surface forming the cavity is made of the cover material C, as shown by reference numeral 9 in FIG.
The cavity is formed in the vertical direction, for example, a structure in which one layer of the base material A is laminated, or a structure in which the base material A, the resin layer X or the resin layer Y, and three layers of the silicone-based resin film described later are laminated. Therefore, it is preferably a rectangular parallelepiped shape. The width of the cavity is desired to be the width at which the liquid sample is introduced stably and at high speed by the capillary phenomenon. The width of the cavity depends on the surface tension of the liquid sample and the thickness of the base material A, but is preferably smaller than 50 mm in order to obtain the effect of the capillary phenomenon. Further, the width of the cavity is preferably larger than 0.1 mm because a width capable of introducing the liquid sample is required. The length of the cavity is not particularly limited, but is preferably 3 mm or more and 20 mm or less. If it is shorter than 3 mm, the variation at the time of inspection may become large. If it is longer than 20 mm, not only the introduction speed of the liquid sample is not stable, but also the required amount of the liquid sample may increase due to the large volume. The length of the cavity in the plane direction is not particularly limited, but it is preferably 10 mm or less, and more preferably 7 mm or less in order to reduce the amount of the liquid sample used.

The resin layers X and Y provided on both sides of the base material A having a cavity are mainly used for adhering the base material A and the cover material B, or the surfaces of the two sheets of the base material A and the cover material C, respectively. Be done. The resin layers X and Y used in the present invention are not limited in composition, curing method, solid content, etc., but preferably exhibit sufficient adhesive strength with respect to the base material A and the cover material B. The resin layers X and Y provided on both sides of the base material A may be the same or different. That is, the resin layers X and Y provided on both surfaces of the base material A may have the same composition, curing method, solid content, and the like, or the resin layers X and Y may have different compositions. As the composition of the resin layers X and Y, for example, an adhesive or a hot melt adhesive may be used as a component thereof.

Adhesives refer to coatings that adhere the adherends by applying pressure, while hot melt adhesives refer to coatings that adhere the adherends by heating. Both the adhesive and the hot melt adhesive are adhesives mainly composed of a thermoplastic resin that becomes solid / semi-solid at room temperature and liquid at high temperature, and are in a solution state diluted as a coating liquid with water or an organic solvent. You may. The main component referred to here refers to a component that occupies 80% by mass or more when the entire pressure-sensitive adhesive or hot-melt adhesive is 100% by mass, and can be a component that has the greatest effect on the characteristics of the hot-melt adhesive.
Thermoplastic resins that can be used as the main components of adhesives or hot melt adhesives include polyester resin, (meth) acrylic resin, polyolefin resin, ethylene-vinyl acetate copolymer resin, polyamide resin, chloroprene resin, aramid resin and acrylic urethane. It is preferable to contain at least one resin selected from the group consisting of resins. In particular, since the hot melt adhesive melts at a lower temperature than the thermosetting resin, the heat effect on other members used in the laminate can be suppressed to a small extent. Further, since it is cooled and solidified, it can be joined in a short time and workability is improved.

In the hot melt adhesive, for example, the base material A and the cover material B are arranged via a resin layer (hot melt adhesive), and a heat sealer is used to provide a sealing temperature of 120 ° C., a sealing time of 1.0 second, and a sealing pressure of 0. The base material A and the cover material B are adhered under the condition of .2 MPa. For the laminate of the present invention, the adhesive strength between the base material A and the resin layer Y due to peeling speed of 50 mm / min and 90 ° peeling was evaluated three times according to JIS Z0237: 2009, and the average value was evaluated as the result. It is preferable to show an adhesive strength of .5N / 10mm or more, and more preferably to show an adhesive strength of 5N / 10mm or more. If the adhesive strength is less than 2.5 N / 10 mm, stable bonding may not be possible, and handling of the laminated body may cause peeling of the laminated body from the end portion.
The resin layer X and / or the resin layer Y used in the present invention is composed of a polyester resin, a (meth) acrylic resin, a polyolefin resin, an ethylene-vinyl acetate copolymer resin, a polyamide resin, a chloroprene resin, an aramid resin and an acrylic urethane resin. It preferably contains at least one resin selected from the group. Further, when the resin layer X and / or the resin layer Y is imparted with hydrophilicity, it is preferable to contain a surfactant. The surfactant used is not limited, but it is preferable to contain a nonionic surfactant having a polyalkylene glycol skeleton (hereinafter, the nonionic surfactant having a polyalkylene glycol skeleton is simply surfactant). Sometimes called an agent).

First, as the hot melt adhesive constituting the laminate, it is possible to use a polyester resin or a (meth) acrylic resin as the hot melt adhesive in the above group with the base material A, the cover material B, and the cover material C. It is preferable from the viewpoint of adhesive strength and heat resistance. Further, it is particularly preferable to use a polyester resin or (meth) acrylic resin having a melting point of 40 ° C. or higher and 150 ° C. or lower as a hot melt adhesive. If the melting point is less than 40 ° C, the hot melt adhesive will flow during thermal bonding, and the adhesive may flow to the part that you do not want to bond, and if it is larger than 150 ° C, it may adhere. If a high temperature is not applied during heat bonding, it may not be possible to bond, which may reduce work efficiency or damage the base material A, cover material B, and cover material C, which are the objects to be bonded, due to heat. It may happen. Further, the hot melt adhesive preferably has an aromatic skeleton because the cohesive force of the resin is improved and the adhesive strength of the resin layer is improved. Further, only one kind of the above-mentioned resin may be used as the hot melt adhesive, or a plurality of resins may be used.

Further, the resin layer X and / or the resin layer Y in the present invention preferably contains a nonionic surfactant having a polyalkylene glycol skeleton together with the hot melt adhesive described above. The nonionic surfactant having a polyalkylene glycol skeleton used in the present invention has a polyalkylene glycol skeleton as a hydrophilic substituent and also has a skeleton described later as a hydrophobic substituent. Here, examples of the nonionic surfactant having a polyalkylene glycol skeleton include polyoxyethylene-alkylsulfate-sodium salt such as polyoxyethylene lauryl-sulfate sodium salt, polyoxyethylene-lauryl ether, and the like. Polyoxyethylene-alkyl ethers such as polyoxyethylene-cetyl ether, polyoxyethylene-oleyl ether, polyoxyethylene-stearyl ether, polyoxyethylene-2-ethylhexyl ether, polyoxyethylene-isodecyl ether, etc.
Polyoxyethylene-alkyl ester such as polyoxyethylene-monolaurate, polyoxyethylene-monostearate, polyoxyethylene-monooleate, polyoxyethylene sorbitan-monolaurate, polyoxyethylene sorbitan-monostearate, polyoxy Ethylene sorbitan-monoolate, polyoxyethylene sorbitan-monoolate and other sorbitan ester / ethylene oxide addition type,
Polyoxyethylene-monoglyceride / ethylene oxide addition type such as coconut fatty acid glyceryl,
Polyoxyethylene-Triglyceride / ethylene oxide addition type such as hardened castor oil,
Alkyl such as polyoxyethylene-laurylamine, polyoxyethylene-alkyl (palm) amine, polyoxyethylene-stearylamine, polyoxyethylene-oleylamine, polyoxyethylene-beef alkylamine, polyoxyethylenealkyl-propylene diamine Polyether Amine type polyoxyethylene-monomethyl ether, polyoxyethylene-dimethyl ether, polyoxyethylene-glyceryl ether, polyoxyethylene α, ω-bis-3-aminopropyl-ether, polyethylene glycol-polypropylene glycol-polyethylene glycol ( Surfactants such as block copolymers) can be mentioned, but nonionic surfactants having a polyalkylene glycol skeleton that can be used in the present invention are not limited to this.

Among the nonionic surfactants having a polyalkylene glycol skeleton, a compound having an alkyl substituent as a hydrophobic substituent is more preferable. As a specific example on the market
Pionin D-1004, Pionin D-1007, Pionin D-1706-N, Pionin D-1715-N, Pionin D-1105, Pionin D-1110, Pionin D-1103-D, Pionin D-1105-D, Pionin D -1103-S, Pionin D-1105-S, Pionin D-1107-S, Pionin D-1109-S, Pionin D-1004, Pionin D-1004, Pionin D-1004,
New Calgen D-1203, New Calgen D-1205, New Calgen D-1208, Pionin D-1305-Z, Pionin D-1323-Z, Pionin D-1803, Pionin D-1402, Pionin D-1420, Pionin D- 1504, Pionin D-1508, Pionin D-1518, Pionin D-1106DIR, Pionin D-1110DIR, Pionin D-1107SP3, Pionin D-1301-P, Pionin D-1305-P (all manufactured by Takemoto Oil & Fat Co., Ltd.),
Emargen 102KG, Emargen 103, Emargen 104P, Emargen 105, Emargen 106, Emargen 108, Emargen 109P, Emargen 120, Emargen 123P, Emargen 130K, Emargen 147, Emargen 150, Emargen 210P, Emargen 220, Emargen 306P, Emargen 320P, Emargen 350 , Emargen 404, Emargen 408, Emargen 409PV, Emargen 420, Emargen 430, Emargen 705, Emargen 707, Emargen 709, Emargen 1108, Emargen 1118S-70, Emargen 1135S-70, Emargen 1150S-60, Emargen 2020G-HA, Emargen 2025G (The above is manufactured by Kao Corporation), etc. When the hydrophobic substituent is an alkyl group, it is easy to produce and obtain a surfactant having a different number of carbon atoms in the alkyl group, so that there is an advantage that the HLB value described later can be arbitrarily changed.

Among the compounds represented by the nonionic surfactant having a polyalkylene glycol skeleton, as a specific example commercially available as an allylphenyl ether type compound having an allylphenyl group containing an aromatic as a hydrophobic substituent, pyonine D-6112, New Calgen C-150, New Calgen C-173, New Calgen C-200, New Calgen C-314, New Calgen CP-50, New Calgen CP-80, New Calgen CP-120, New Calgen CP- Examples thereof include 15-200, Pionin D-6512, Pionin D-6414, DTD-51, Pionin D-6315, New Calgen E-200, Pionin D-7240 (all manufactured by Takemoto Oil & Fat Co., Ltd.) and the like. When the hydrophobic substituent is an allylphenyl group, for example, when a hot melt adhesive having an aromatic is used in combination, the effect of improving the cohesive force by stacking the aromatic rings of the hot melt adhesive and the surfactant causes the resin layer to have an effect. This is preferable because it improves the adhesive strength. Further, when the hydrophobic substituent is an allylphenyl group, it is easy to manufacture and obtain a surfactant in which the number of repetitions of the allylphenyl group is arbitrarily adjusted, so that there is an advantage that the HLB value described later can be arbitrarily changed. is there.

Among the compounds represented by the nonionic surfactants having a polyalkylene glycol skeleton, specific examples of commercially available sorbitan fatty acid derivatives are Leodore TW-L120, Leodore TW-L106, Leodore TW-P120, and Leodore TW. -S120V, Leodor TW-S106V, Leodor TW-S-320V, Leodor TW-O120V, Leodor TW-O106V, Leodor TW-O320V, Leodor TW-IS399C, Leodor Super TW-L120 (all manufactured by Kao Corporation), Examples thereof include Pionin D-941, Pionin D-945, Pionin D-945T (all manufactured by Takemoto Oil & Fats Co., Ltd.) and the like.

The number average molecular weight (Mn) of the nonionic surfactant having the polyalkylene glycol skeleton constituting the laminate is preferably 500 to 20,000, more preferably 2,000 to 20,000, particularly preferably. Is 5,000 to 18,000. That is, when the number average molecular weight is 500 or more, the surfactant component adheres to the surface and cross section of the transport roll and the slit blade in the transport step, the printing process, the slit process, and the like when the laminate is rolled. This can be satisfactorily prevented, and as a result, the cleaning frequency of each step is significantly reduced, which is preferable. When the number average molecular weight is 20,000 or less, the surfactant can be easily dispersed uniformly in the resin layer, which is preferable.

The melting point or freezing point of the surfactant contained in the resin layer X and the resin layer Y is not particularly limited, but is preferably 30 ° C. or lower. The melting point or freezing point is more preferably 25 ° C. or lower, still more preferably 20 ° C. or lower. If the melting point or the freezing point is larger than 30 ° C., the surfactant tends to bleed out from the resin layer, and the adhesive strength of the hot melt adhesive varies, which may lead to a decrease in the adhesive strength. Further, when the melting point or the freezing point is 30 ° C. or lower, it is preferable from the viewpoint of workability because the heat-melting work of the surfactant becomes unnecessary when preparing the coating liquid in which the hot melt adhesive and the surfactant are mixed. ..

In the resin layer X and / or the resin layer Y in the present invention, the amount of the surfactant is 0.1 to 1 with respect to 100 parts by mass of the resin contained in the resin layer X and the resin layer Y for each of the resin layer X and the resin layer Y. It is preferably 20 parts by mass, more preferably 0.1 to 12 parts by mass, and further preferably 1 to 10 parts by mass. If the amount of the surfactant is less than 0.1 parts by mass, the hydrophilicity of the resin layer may not be sufficiently obtained, and the introduceability of the liquid sample may not be sufficiently exhibited. In addition, the effect of preventing the main component contained in the resin layer from adhering may not be sufficiently exhibited in the laminate transport step or the slit step.
If the amount of the surfactant is larger than 20 parts by mass, it is difficult to obtain sufficient compatibility with the hot melt adhesive, the amount of the surfactant that bleeds out to the surface of the resin layer increases, and the adhesiveness of the hot melt adhesive is impaired. It may not be possible to obtain the desired adhesive strength. Further, if the amount of the surfactant is larger than 20 parts by mass, the surfactant component may adhere in the laminate transport step, the slit step, or the like due to the bleed-out of the surfactant, and the processing step may be contaminated. Further, if the amount of the surfactant is larger than 20 parts by mass, the blending ratio of the hot melt adhesive decreases, so that the strength of the resin layer decreases, which not only makes stable film formation difficult, but also the interface with the hot melt adhesive. It may lead to a decrease in storage stability of the paint mixed with the activator. When the amount of the surfactant is 0.1 to 20 parts by mass, it is preferable because a resin layer having the above-mentioned drawbacks solved can be obtained.

Examples of the hot melt adhesive include Aronmelt PES-120L, PES-140H, PES-111EE, PES310S30, PES375S40, PPET1008, PPET1025, PPET2102, PPET1303S (all manufactured by Toyobo Co., Ltd.), Y-167, H-930. -S, H-180S (all manufactured by Tanaka Chemical Co., Ltd.), Nichigo Polyester (R) SP-154, SP-165, SP-170, SP-176, SP-180, SP-182, SP-185 ( Above, Nippon Synthetic Chemical Co., Ltd.), Byron (R) 200, 240, 300, 550, BX1001 (above, manufactured by Toyobo Co., Ltd.), Polysol (R) SE-1720, SE-4210E, SE-6210, SE- 6210L (above, manufactured by Showa Denko KK) and the like.

The water contact angle on the surface of at least one of the resin layer X and the resin layer Y in the present invention is preferably less than 15 degrees. It is preferable that the contact angle with water is less than 15 degrees because high-speed and stable introduction of the liquid sample can be sufficiently realized. It is preferable that the water contact angle on the surface of at least one of the resin layer X and the resin layer Y is less than 15 degrees, and more preferably the water contact angle on the surfaces of both resin layers is less than 15 degrees.

The surfactant used in the present invention is preferably a surfactant having an HLB value of 8 to 15. It is preferable to use a surfactant having an HLB value of 8 to 15 because it is possible to impart hydrophilicity to the resin layer while maintaining compatibility with the hot melt adhesive.
The HLB (HyDropfile-Lipophile Balance) value is a hydrophilic-lipophilic balance, and is a value calculated by the Griffin method (completely revised edition, Introduction to Surfactants, p128) obtained from the following formula (1).
HLB value of surfactant = (number average molecular weight of hydrophilic group portion / number average molecular weight of surfactant) × 20 formula (1)
When the HLB value of the surfactant is larger than 15, it is difficult to obtain sufficient compatibility between the surfactant and the main component of the resin layer, and the amount of the surfactant that bleeds out to the surface of the resin layer increases, so that the hot melt adhesive Adhesiveness may be impaired and the desired adhesive strength may not be obtained. On the other hand, if the HLB value of the surfactant is less than 8, the hydrophilicity of the resin layer may not be sufficiently obtained. When the HLB value of the surfactant is 8 to 15, it is preferable because it is possible to achieve both compatibility with the hot melt adhesive and hydrophilicity of the resin layer.

The amount of adhesion of the resin layer X and the resin layer Y is preferably ¹ to 50 g / m ² on one side, and more preferably ² to 30 g / m ² . If the amount of adhesion is less than 1 g / m ^{2, the} adhesive layer may come off due to scratches during processing, pinholes may occur, or the desired adhesive strength may not be obtained. As a result, the adhesive strength may vary. Further, when the adhesion amount is more than 50 g / m ^2, the effect of preventing the hot melt adhesive component from adhering in the laminate transfer step, the slit step, or the like may be reduced.

Next, the means for producing the laminate of the present invention will be described below. The resin layer X and the resin layer Y can be formed, for example, by applying a coating liquid containing a component constituting the resin layer to the cover material B and the cover material C to form a coating film. When imparting hydrophilicity, such a coating liquid can be obtained, for example, by mixing a hot melt adhesive and a surfactant, or by thermally melting the mixture.
The coating method of the coating liquid is not particularly limited, but methods such as a gravure coating method, a reverse coating method, a kiss coating method, a die coating method, and a bar coating method can be used. The coating film concentration, coating film drying conditions, or coating film cooling conditions are not particularly limited, but it is desirable that the coating film drying conditions be performed within a range that does not adversely affect various characteristics of the substrate.

Further, the resin layer X and the resin layer Y of the present invention can also be formed by, for example, forming a film-like material in advance with the above coating liquid and laminating it with the cover material B or the cover material C. In the case of bonding, a method of applying a coating liquid to a release film such as a silicone-based resin film and transferring it to a cover material is adopted (hereinafter, a two-layer structure of a cover material and a resin layer is covered film. It may be called.).
Next, the base material A having a through hole penetrating in the thickness direction, the previously formed resin layer X, and the resin layer Y are combined and bonded by heat treatment. The heat treatment method is not particularly limited, but heating. A press or a heat sealer (heating roller) can also be used. Then, the cover material B or the cover material C separately prepared is bonded to the back surface of the base material A by heat treatment, whereby the laminate of the present invention can be obtained. When the through holes penetrating in the thickness direction are simultaneously provided in the resin layer X, the resin layer Y, and the base material A, the coating liquid is formed not in the cover material B or the cover material C but in the base material A, and the base material is formed. A laminated body is obtained by attaching a cover film to the surface on the A side and another cover material on the resin layer side by heat treatment.

Hereinafter, the present invention will be specifically described with reference to Examples, but the present invention is not limited to these Examples. In the examples, the method for evaluating the characteristics of the test piece is as follows.

[Contact angle of the surface of the resin layer with water]
The contact angle of the resin layer with water is 5 seconds after the droplets formed when pure water (2.0 μl) is dropped on the surface of the resin layer using DM-400s (manufactured by Kyowa Interface Science Co., Ltd.). The water contact angle on the surface of the resin layer was measured 5 times, and the average value was used as the result.

[Adhesive strength of resin layer]
Regarding the laminated body, the adhesive strength of the cover material provided with the resin layer and the base material A was evaluated three times under the conditions of a peeling angle of 90 ° and a peeling speed of 50 mm / min according to JIS Z0237: 2009, and the average value thereof. Was the result.

[Number average molecular weight of resin]
Using GPC, the molecular weight based on polystyrene was calculated under the following conditions. Equipment: HLC-8220 GPC (manufactured by Tosoh), column: 2 TSKgel GMHXL (manufactured by Tosoh), column temperature: 40 ° C, eluent: tetrahydrofuran 1.00 ml / min, detector: RI.

[Adhesion amount of resin layer]
The mass of the base material 100 cm ² coated with the coating liquid was measured (X), then the mass of the base material 100 cm ² before coating was measured (Y), and the adhesion amount (g / m) was (XY) × 100. ² ) was calculated.

[Definiteness and quantification of surfactant contained in resin layer]
The qualification and quantification of the surfactant contained in the resin layer can be performed by analysis using LC / MS / MS according to the following procedure. LC / MS / MS is a mass spectrometry method applicable to non-volatile compounds such as surfactants, which are difficult to analyze by LC / MS (gas chromatograph mass spectrometer).

<Preparation of a solution of surfactant contained in the resin layer>
(1) Remove the resin layer from the cover film and weigh 0.04 g of it into a 25 mL volumetric flask.
(2) Add 1 mL of HFIP (1,1,1,1,3,3,3-hexafluoro-2-propanol) / chloroform (1/1) to the volumetric flask to dissolve the resin layer.
(3) After adding 2 mL of chloroform, acetonitrile is gradually added to insolubilize the hot melt adhesive component.
(4) Add acetonitrile to a volume of 25 mL, and then dilute the prepared solution 100-fold with acetonitrile.
(5) The prepared 100-fold diluted solution is filtered with a PTFE disc filter (0.45 μm), and the obtained filtrate is used as a measurement solution.

<Definiteness of surfactant contained in resin layer>
(6) The solution obtained in step (5) is subjected to LC / MS / MS, and the retention time and peak area where the peak derived from the surfactant is detected from the chromatogram are confirmed.
(7) By performing MS analysis on the peak derived from the surfactant, the formula amount of the positive ion derived from the surfactant is confirmed.

<Preparation of standard solution and preparation of calibration curve>
(8) Weigh the standard (0.01 g) of the surfactant qualified in steps (6) and (7) into a 10 mL volumetric flask, dissolve it in methanol, and set the volume to 10 mL to make a standard solution. To do.
(9) The standard solution is separated and diluted with methanol to obtain a total of four standard solutions having arbitrary concentrations.
(10) By subjecting the standard solution obtained in step (9) to LC / MS / MS, respectively, the peak area of the chromatogram for each concentration is confirmed.
(11) A calibration curve is obtained by linearly approximating the relationship between the solution concentration and the peak area.

<Quantification of surfactant contained in resin layer>
(12) The content of the surfactant is calculated by substituting the peak area grasped in the procedure (6) into the equation of the calibration curve obtained in the procedure (11) and calculating the concentration of the surfactant contained in the resin layer. Ask for.

The procedures (1) to (7) and (11) and (12) are carried out at n = 2, and the average value thereof is used as the result.
In this analysis, LC system: LC-20A (manufactured by Shimadzu Corporation), MS system: API4000 (manufactured by AB SCIEX Co., Ltd.), column: Ionization ODS-3 (2.1 x 150 mm, 5 μm) (GL Sciences Co., Ltd.) (Manufactured by), column temperature: 50 ° C, flow rate: 0.25 mL / min, injection volume: 1 μL, ionization method: APCI (atmospheric pressure chemical ionization method), detection: positive ion detection, measurement mode: SRM (Selected reaction monitoring) ) Conditions.

(Example 1)
As a resin constituting the hot melt adhesive, a 20% by mass solution prepared by dissolving a polyester adhesive resin having a melting point of 100 ° C. and a number average molecular weight of 22,000 in toluene is prepared, and a nonionic surfactant having a polyalkylene glycol skeleton is further prepared. [Leodor TW-L106 (HLB value: 13.3) manufactured by Kao Co., Ltd.] was prepared, and the coating liquid was prepared so that the solid content conversion ratio was 100 parts by mass / 4 parts by mass. This coating liquid is applied to the silicone-based resin coated surface of the silicone-based resin film with a comma coater and dried at 120 ° C. for 30 seconds to provide a resin layer 1a with an adhesion amount of 45 g / m ² on one side, and then Toray Co., Ltd. A cover film 1A was obtained by transferring to a polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 100S28) manufactured by the company and peeling off the silicone-based resin film. The coating thickness of the resin layer 1a was 20 μm.

Next, a through hole penetrating in the thickness direction of 10 mm in width by laser processing (a through hole penetrating in the thickness direction of 10 mm in width by laser processing is a shape like the base material A shown by reference numeral 1 in FIG. The resin layer surface of the cover film 1A is aligned with the polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 100E20) manufactured by Toray Industries, Inc., which is provided with the part processed by the above. It was pasted under the conditions. The 100E20 surface of the obtained sample and the resin layer surface of another cover film 1A prepared separately were combined and bonded again using a heat sealer under the above conditions to obtain the laminate 1 of the present invention. The laminated body 1 has a configuration corresponding to FIGS. 1 and 2, and the laminated body 1 has the configuration of the aspect of the present invention 1.

(Example 2)
In Example 1, a 20% by mass solution in which a piece of resin layer 1a is used as a resin constituting a hot melt adhesive and a polyester adhesive resin having a melting point of 100 ° C. and a number average molecular weight of 22,000 is dissolved in toluene is prepared. The laminate 2 of the present invention was obtained in the same manner except that the resin layer 2b obtained in the above was used. The laminated body 2 has a configuration corresponding to FIGS. 1 and 2, and the laminated body 2 has the configuration of the aspect of the present invention 1.

(Example 3)
After providing the resin layer 2b on the silicone-based resin film, a polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 100E20) manufactured by Toray Industries, Inc. is attached to the surface of the resin layer 2b, and a punching method using a Thomson mold is used. A through hole penetrating in the thickness direction having a width of 10 mm was provided. Then, the silicone-based resin film was peeled off to obtain a spacer film 3. After laminating the resin layer 2b surface of the obtained spacer film 3 and the polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 100S28) manufactured by Toray Industries, Inc. under the conditions of Example 1 using a heat sealer. The laminate 3 of the present invention was obtained by laminating the resin layer 1a surface of the cover film 1A and the spacer base material of the spacer film 3 separately prepared by using a heat sealer under the conditions of Example 1. The laminate 3 has a configuration corresponding to FIGS. 3 and 4, and the laminate 3 has the configuration of the aspect of the present invention 2.

(Example 4)
In Example 3, a magnetron sputtering device was used on the bonding surface side of a polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 188E20) manufactured by Toray Industries, Inc., and a palladium layer having a thickness of 10 nm was used as a film base material. The laminate 4 of the present invention was obtained in the same manner except that the cover material 4 formed on one surface was provided on the resin layer 2b surface of the spacer film 3. Palladium having a purity of 99.9% by mass (manufactured by Tanaka Kikinzoku Kogyo Co., Ltd.) was used as the target for magnetron sputtering, and argon was used as the sputtering gas. The laminated body 4 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 4 has the configuration of the aspect of the present invention 2.

(Example 5)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Leodol TW-L106 (HLB value: 13.3) manufactured by Kao Corporation] is used as a ratio of 8 parts by mass in terms of solid content of a hot melt adhesive. The laminate 5 of the present invention was obtained in the same manner except that the coating liquid was prepared as described above. The laminated body 5 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 5 has the configuration of the aspect of the present invention 2.

(Example 6)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1508 (HLB value: 11.4) manufactured by Takemoto Oil & Fat Co., Ltd.] was added to a ratio of 4 parts by mass in terms of solid content of the hot melt adhesive. The laminate 6 of the present invention was obtained in the same manner except that the coating liquid was prepared so as to be. The laminated body 6 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 6 has the configuration of the aspect of the present invention 2.

(Example 7)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1508 (HLB value: 11.4) manufactured by Takemoto Oil & Fat Co., Ltd.] was added to a ratio of 3 parts by mass in terms of solid content of the hot melt adhesive. The laminate 7 of the present invention was obtained in the same manner except that the coating liquid was prepared so as to be. The laminated body 7 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 7 has the configuration of the aspect of the present invention 2.

(Example 8)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1508 (HLB value: 11.4) manufactured by Takemoto Oil & Fat Co., Ltd.] was added to a ratio of 20 parts by mass in terms of solid content of the hot melt adhesive. The laminate 8 of the present invention was obtained in the same manner except that the coating liquid was prepared so as to be. The laminated body 8 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 8 has the configuration of the aspect of the present invention 2.

(Example 9)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1105-S (HLB value: 10.5) manufactured by Takemoto Oil & Fat Co., Ltd.] was used in a solid content conversion ratio of 4 parts by mass of a hot melt adhesive. The laminate 9 of the present invention was obtained in the same manner except that the coating liquid was prepared so as to have a ratio. The laminated body 9 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 9 has the configuration of the aspect of the present invention 2.

(Example 10)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1105-S (HLB value: 10.5) manufactured by Takemoto Oil & Fats Co., Ltd.] was used as a solid content conversion ratio of 8 parts by mass of a hot melt adhesive. The laminate 10 of the present invention was obtained in the same manner except that the coating liquid was prepared so as to have a ratio. The laminate 10 has a configuration corresponding to FIGS. 3 and 4, and the laminate 10 has the configuration of the aspect of the present invention 2.

(Example 11)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Emargen 103 (HLB value: 8.1) manufactured by Kao Corporation] was used so as to have a solid content conversion ratio of 4 parts by mass of the hot melt adhesive. The laminate 11 of the present invention was obtained in the same manner except that the coating liquid was prepared. The laminated body 11 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 11 has the configuration of the aspect of the present invention 2.

(Example 12)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Emargen 103 (HLB value: 8.1) manufactured by Kao Corporation] was used so as to have a solid content conversion ratio of 8 parts by mass of the hot melt adhesive. The laminate 12 of the present invention was obtained in the same manner except that the coating liquid was prepared. The laminated body 12 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 12 has the configuration of the aspect of the present invention 2.

(Example 13)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [New Calgen D-1205 (HLB value: 10.5) manufactured by Takemoto Oil & Fat Co., Ltd.] was used as a ratio of 4 parts by mass in terms of solid content of a hot melt adhesive. The laminate 13 of the present invention was obtained in the same manner except that the coating liquid was prepared so as to be. The laminated body 13 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 13 has the configuration of the aspect of the present invention 2.

(Example 14)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [New Calgen D-1205 (HLB value: 10.5) manufactured by Takemoto Oil & Fat Co., Ltd.] was used as a ratio of 8 parts by mass in terms of solid content of a hot melt adhesive. The laminate 14 of the present invention was obtained in the same manner except that the coating liquid was prepared so as to be. The laminated body 14 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 14 has the configuration of the aspect of the present invention 2.

(Example 15)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [DTD-51 (HLB value: 13.7) manufactured by Takemoto Oil & Fat Co., Ltd.] is used as a ratio of 4 parts by mass in terms of solid content of the hot melt adhesive. The laminate 15 of the present invention was obtained in the same manner except that the coating liquid was prepared as described above. The laminated body 15 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 15 has the configuration of the aspect of the present invention 2.

(Example 16)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [DTD-51 (HLB value: 13.7) manufactured by Takemoto Oil & Fat Co., Ltd.] has a solid content conversion ratio of 8 parts by mass of a hot melt adhesive. The laminate 16 of the present invention was obtained in the same manner except that the coating liquid was prepared as described above. The laminated body 16 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 16 has the configuration of the aspect of the present invention 2.

(Example 17)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1007 (HLB value: 14.1) manufactured by Takemoto Oil & Fat Co., Ltd.] was added to a ratio of 4 parts by mass in terms of solid content of the hot melt adhesive. The laminate 17 of the present invention was obtained in the same manner except that the coating liquid was prepared so as to be. The laminated body 17 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 17 has the configuration of the aspect of the present invention 2.

(Example 18)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1007 (HLB value: 14.1) manufactured by Takemoto Oil & Fat Co., Ltd.] was added to a ratio of 8 parts by mass in terms of solid content of the hot melt adhesive. The laminate 18 of the present invention was obtained in the same manner except that the coating liquid was prepared so as to be. The laminated body 18 has a configuration corresponding to FIGS. 3 and 4, and the laminated body 18 has the configuration of the aspect of the present invention 2.

(Comparative Example 1)
On 100 parts by mass of polyester resin on polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 100S28) manufactured by Toray Industries, Inc., a cationic surfactant (Anstex C-200X manufactured by Toho Chemical Industry Co., Ltd.) ) Was prepared in a proportion of 5 parts by mass and applied with a gravure coater to obtain a cover film 19A having a hydrophilic coat layer 11.
Next, a polyester-based adhesive resin is applied to the silicone-based resin coated surface of the silicone-based resin film with a comma coater to provide a hot melt resin layer, and then a polyethylene terephthalate (PET) film "Lumirror" (registered) manufactured by Toray Co., Ltd. Transferred to (trademark) (type 100E20). A hot melt resin layer was provided on the silicone resin coated surface of the silicone resin film in the same procedure, and the hot melt resin layer was transferred to the other surface of 100E20. Further, a spacer film 19B was obtained by providing a through hole penetrating in the thickness direction.
Next, a palladium layer was formed on one surface of a polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 188E20) manufactured by Toray Industries, Inc. using a magnetron sputtering apparatus.
The hydrophilic coat surface of the cover film 19A and the hot melt resin layer surface of the spacer film 19B were bonded together using a heat sealer under the above-mentioned conditions. The laminate 19 of the present invention was obtained by aligning the other hot-melt resin layer surface of the obtained sample with the previous palladium surface and laminating them again using a heat sealer under the above-mentioned conditions. The laminated body 19 has a structure having the hydrophilic coat layer 11 corresponding to FIG. 6, and does not have the structure of the laminated body specified in claims 1 and 2. Note that FIG. 6 is a cross-sectional view of the laminate cut in the thickness direction between the introduction port and the opposite side of the introduction port (that is, the surface facing the introduction port) and viewed from the introduction port side.
When the thicknesses of the base material A, the resin layer X, and the resin layer Y are constant, the height of the cavity is kept low in Examples 1 to 18 corresponding to FIG. 3 as opposed to Comparative Example 1 corresponding to FIG. Therefore, the amount of liquid sample used can be reduced. Further, since the hydrophilic coat layer is not provided in Examples 1 to 18 as compared with Comparative Example 1, the manufacturing process is simplified.

According to the present invention, there is provided a laminate suitable for a biosensor in which the amount of a liquid sample used is reduced and the manufacturing process is simplified.

Although the present invention has been described in detail and with reference to specific embodiments, it will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention.
This application is based on a Japanese patent application filed on October 20, 2017 (Japanese Patent Application No. 2017-203166), the contents of which are incorporated herein by reference.

1: Base material A (spacer base material)
21: Cover material B
22: Cover material C
31: Resin layer X
32: Resin layer Y
4: Cavity 41: Introduction port 5: Cover film 6: Laminated body 7a: Surface 7b composed of resin layer X: Surfaces 8a and 8b composed of resin layer Y: Surface 9 composed of only base material A: Surfaces 10a and 10b made of cover material C: Surfaces made of only base material A and resin layer Y 11: Hydrophilic coat layers 12a and 12b: Surfaces made of base material A, resin layer X and resin layer Y

Claims (7)

A laminate having a cover material B, a resin layer X, a base material A, a resin layer Y, and a cover material C in this order, the laminate having a cavity having an introduction port, and the cavity being the following ( A laminate characterized by being composed of a surface (i), a surface (ii), and a surface (iii).
(I) One surface composed of the resin layer X (ii) One surface composed of the resin layer Y (iii) Three surfaces composed of only the base material A A laminate having a cover material B, a resin layer X, a base material A, a resin layer Y, and a cover material C in this order, the laminate having a cavity having an introduction port, and the cavity being the following ( A laminate characterized by being composed of a surface of iv), a surface of (v), and a surface of (vi).
(Iv) One surface composed of the resin layer X (v) Three surfaces composed of only the base material A and the resin layer Y (vi) One surface composed of the cover material C The laminate according to claim 1 or 2, wherein the water contact angle of the surface of at least one of the resin layer X or the resin layer Y is less than 15 degrees. The laminate according to any one of claims 1 to 3, wherein the adhesive strength between the base material A and the resin layer Y at a peeling angle of 90 ° in accordance with JIS Z0237: 2009 is 2.5 N / 10 mm or more. .. The laminate according to any one of claims 1 to 4, wherein the resin layer X and / or the resin layer Y contains a surfactant. The resin layer X and / or the resin layer Y is selected from the group consisting of polyester resin, (meth) acrylic resin, polyolefin resin, ethylene-vinyl acetate copolymer resin, polyamide resin, chloroprene resin, aramid resin and acrylic urethane resin. The laminate according to any one of claims 1 to 5, which contains at least one resin. The laminate according to claim 5 or 6, wherein the surfactant is a nonionic surfactant having a polyalkylene glycol skeleton.

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