JPWO2019059162A1 - How to sterilize the surface of bird eggs - Google Patents

Abstract

Many bacteria are attached to the surface of the bird egg as it is laid. Therefore, there is a demand for a method for sterilizing the surface of a bird egg, which can efficiently sterilize the surface of the bird egg in a short time and has few problems with drainage standards. The present invention provides a method for sterilizing a bird egg surface, which comprises contacting a bird egg surface with a peracetic acid solution. The concentration of the peracetic acid is preferably 80 to 1000 ppm, and the surface of the bird egg and the peracetic acid solution are preferably brought into contact with each other at 10 to 40 ° C. for 5 seconds to 20 minutes.

Description

The present invention relates to a method for sterilizing the surface of a bird egg.
The present application claims priority based on Japanese Patent Application No. 2017-196406 filed in Japan on September 21, 2017, the contents of which are incorporated herein by reference.

Chicken spawning begins with ovulation, which occurs after the formation of mature follicles, and ovulation occurs through the funnel, ampulla, narrow, eggshell glands, and eggshell vagina, and through the cloaca. At this time, in order to facilitate spawning, mucus of a protein containing sugar is secreted in the vaginal part of the eggshell and adheres to the surface to smooth the surface. By passing through the cloaca during this spawning process, the bacteria come into contact with the intestinal bacteria, and the bacteria adhere to the cuticle layer composed of proteins on the surface of the eggshell.

In this way, even if it is clean to the naked eye, germs are attached to the surface of the eggshell due to the opportunity of contact with intestinal bacteria. Escherichia coli and staphylococci are also problems, but Campylobacter and Salmonella are particularly problematic, and their removal is an issue.

As the washing and sterilizing methods that have been performed so far, a method using sodium hypochlorite, a method using ozone, a method using an electrolyte, and the like are known.
Patent Document 1 discloses an apparatus in which eggs are washed with acetic acid and then washed with weakly acidic electrolyzed water having a chlorine concentration of 200 ppm and a pH of 5.5. Further, Patent Document 2 discloses a method of spraying water, washing with a brush, and then sterilizing using hypochlorous acid water and hypochlorous acid water.

Japanese Unexamined Patent Publication No. 2005-027609 Japanese Unexamined Patent Publication No. 2003-023907

The sterilization method using sodium hypochlorite has a problem of impact on the human body and a problem of wastewater standards as well as a water washing process because sodium hypochlorite has a residual property. In addition, the sterilization method using ozone also has problems of affecting the human body and corrosiveness. As for the sterilization method using electrolyzed water, since chlorine is also used as the electrolyte, the problem of wastewater standards remains.

In addition, it is necessary to increase the number of chickens in order to increase the production of chicken eggs. In that case, a fertilized egg is required, and the surface of the chicken egg needs to be sterilized. In particular, contamination with Salmonella is a problem, and incubating while contaminated results in the birth of contaminated parent birds. However, if the usual washing method by immersion is used, the surface of the egg will be covered with a water film, which will hinder the respiratory action performed through the fine holes on the surface, and in some cases, the egg will die. May cause. In such a case, a method is adopted in which a non-woven fabric is used, soaked with a bactericidal agent, squeezed, and the surface is wiped off. In addition, since this fertilized egg is also used for vaccine production, this work is a very important process, but depending on the fungicide, sterilization may be insufficient or it may take time.

Therefore, an object of the present invention is to provide a method for sterilizing a bird's egg surface, which can efficiently sterilize the surface of a bird's egg in a short time and has few problems of drainage standards.

By using a peracetic acid solution to sterilize the surface of chicken eggs and the like, the present inventors can sterilize the surface of chicken eggs efficiently in a short time, and there is also a problem of drainage standards. We found that there were few, and completed the present invention.
That is, the present invention provides a method for sterilizing the surface of a bird egg having the following characteristics.
[1] A method for sterilizing a bird egg surface, which comprises contacting the bird egg surface with an aqueous solution of peracetic acid.
[2] The method according to [1], wherein the concentration of peracetic acid is 80 to 1000 ppm.
[3] The method according to [1] or [2], wherein the surface of the bird egg and the peracetic acid solution are brought into contact with each other at 10 to 40 ° C. for 5 seconds to 20 minutes.
[4] The method according to any one of [1] to [3], wherein the peracetic acid solution is an equilibrium peracetic acid solution composed of glacial acetic acid and hydrogen peroxide.
[5] The method according to any one of [1] to [4], wherein the contact between the bird egg surface and the peracetic acid solution is by spraying the peracetic acid solution onto the bird egg surface.
[6] The method according to any one of [1] to [5], wherein the contact between the bird egg surface and the peracetic acid solution is performed by stroking the bird egg surface with a non-woven fabric containing the peracetic acid solution.
[7] The method according to any one of [1] to [6], wherein the bird egg is an unfertilized egg or a fertilized egg.
[8] The method according to any one of [1] to [7], wherein the bird egg is a chicken egg, a quail egg, or a duck egg.

Peracetic acid has a strong oxidizing action, and its oxidizing power is said to be 10,000 times that of hydrogen peroxide, and the oxidizing power produces a strong bactericidal action. This bactericidal activity has already been recognized, and is generally used for surface sterilization of foods such as vegetables, fruits, fish, and meat in the United States and other countries, and is also approved as a food additive in Japan. Sterilization with peracetic acid does not use chlorine and consists of volatilizing components, so it can be left to dry to a certain extent, so there is no persistence and there is no need to wash it with water after use. The amount of water used is significantly reduced. In addition, the waste solution after use of the appropriately diluted peracetic acid solution is discharged in fixed amounts according to the running water washing method, but the discharged waste solution is the case of the peracetic acid solution produced by the equilibrium reaction. It is hard to be deactivated and contributes to sterilization of the floor surface in the factory and the drainage ditch in the facility. Ultimately, it is mixed and neutralized with wastewater discharged for other purposes, so there is less impact on sewage purification microorganisms in the treatment tank and problems with wastewater standards.

However, although peracetic acid is used for surface sterilization of foods such as vegetables, fruits, fish and meat, it has not been used for sterilization of the surface of birds and eggs, and it is unknown whether it is applicable. ..

According to the present invention, there is provided a method for sterilizing a bird egg surface, which comprises contacting a bird egg surface with an aqueous peracetic acid solution. According to the method for sterilizing the surface of a bird egg of the present invention, the surface of the bird egg can be sterilized efficiently in a short time without any problem of drainage standard.

It is a figure which showed an example of the apparatus which sprays a peracetic acid solution on the surface of a bird egg. It is a figure which showed the detection result of the number of bacteria existing on the eggshell surface immediately after spawning. The vertical axis shows the number of eggs, and the horizontal axis shows the log value of the number of bacteria.

The method for sterilizing the surface of a bird egg of the present invention is characterized in that the surface of the bird egg is brought into contact with an aqueous solution of peracetic acid. The bird egg may be an unfertilized egg or a fertilized egg. The fertilized egg can be used for the production of raw birds such as chickens, vaccine production, and the like.

The concentration of peracetic acid used in the method for sterilizing the surface of a bird egg of the present invention is not particularly limited as long as the surface of the bird egg can be sterilized, but is preferably 50 to 1000 ppm, more preferably 80 to 500 ppm, and further preferably 80. It can be ~ 200 ppm. The higher the concentration of peracetic acid, the shorter the time it takes to sterilize the surface of the bird egg.

The peracetic acid solution used in the method for sterilizing the surface of chicken eggs of the present invention is irreversible, which is produced by an equilibrium reaction caused by mixing acetic acid and hydrogen peroxide, or by mixing triacetin and hydrogen peroxide. Can be produced by a similar reaction.
Since peracetic acid produced by mixing acetic acid and hydrogen peroxide is subjected to an equilibrium reaction, even if peracetic acid is used in the method for sterilizing bird eggs of the present invention, hydrogen peroxide and acetic acid are produced by the equilibrium reaction. Reacts and the amount of peracetic acid is maintained, so that the effect of peracetic acid in the method for sterilizing bird eggs of the present invention can be maintained for a long period of time. As acetic acid, glacial acetic acid is preferably used because it has excellent reactivity and can improve the bactericidal effect of the present invention.

The peracetic acid solution may contain other components such as 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP), if desired. HEDP has the effect of stabilizing peracetic acid in the peracetic acid solution. The concentration of HEDP in the peracetic acid solution is preferably less than 1%, more preferably 0.6 to 0.9%.

The contact between the bird egg surface and the peracetic acid solution is not particularly limited as long as the bird egg surface and the peracetic acid solution can come into contact with each other. For example, a method of immersing the bird egg in the peracetic acid solution or spraying the peracetic acid solution on the bird egg surface. A method of stroking the surface of a bird egg with a non-woven fabric containing a peracetic acid solution, and the like. In particular, when the bird egg is an unfertilized egg, a method of immersing the bird egg in a peracetic acid solution or a method of spraying the peracetic acid solution on the surface of the bird egg is preferable, and when the bird egg is a fertilized egg, the method is preferable. A method of stroking the surface of the bird egg with a non-woven material containing a peracetic acid solution is preferable. This is because when the bird egg is immersed in the peracetic acid solution or the peracetic acid solution is sprayed on the surface of the bird egg for a long time, the surface of the bird egg may be covered with the peracetic acid solution, and the surface of the bird egg is fine. This is because it interferes with the breathing through the hole, and in some cases, the fertilized egg may die.

FIG. 1 shows an example of a peracetic acid solution spraying device that sprays a peracetic acid solution on the surface of a bird egg. As shown in FIG. 1, in the peracetic acid solution spraying device 10, while rotating the bird egg 4 on the belt conveyor 3, the peracetic acid solution is sprayed from the upper spray nozzle 1 and then under the rotating brush 2. Let it pass. Instead of the rotating brush 2, felt or the like containing a peracetic acid solution may be used.
By using the peracetic acid solution spraying device 10, the contact time between the bird egg surface and the peracetic acid solution can be lengthened, and the bird egg surface can be sterilized efficiently and automatically and continuously. ..

In the case of the method of stroking the surface of a bird's egg with a non-woven fabric containing a peracetic acid solution, the surface of the bird's egg can be sterilized by impregnating the non-woven fabric with the peracetic acid solution and gently wiping it in a sufficiently squeezed state. In addition, felt or the like may be used instead of the said non-woven fabric.

In the case of unfertilized eggs, the temperature at which the surface of the bird egg is brought into contact with the peracetic acid solution varies depending on the climate, but is preferably 10 to 40 ° C., and in accordance with general hygiene standards, is 30 ° C. or higher on the surface of the bird egg before washing. A temperature 5 ° C. or higher higher than the temperature is more preferable. In the case of fertilized eggs, the temperature is preferably 34 to 37 ° C, more preferably about 5 ° C higher than the temperature of the surface of the bird egg before washing.

The pH at which the surface of the bird egg is brought into contact with the peracetic acid solution is not particularly limited, but is preferably pH 2 to 6, and more preferably pH 2 to 4.

The time for contacting the surface of the bird egg with the peracetic acid solution means the time during which the surface of the bird egg is wet with the peracetic acid solution, and the time is not particularly limited, but when the bird egg is immersed in the peracetic acid solution. , And when the peracetic acid solution is sprayed on the surface of the bird egg, it is preferably 5 seconds to 20 minutes, more preferably 1 minute to 10 minutes, particularly preferably 1 minute to 5 minutes, and the non-woven fabric containing the peracetic acid solution. When stroking the surface of the bird egg, it is preferably 5 seconds to 5 minutes, more preferably 30 seconds to 3 minutes, and particularly preferably 1 minute to 3 minutes. The higher the concentration of the peracetic acid solution, the shorter the contact time between the bird egg surface and the peracetic acid solution.

After contacting the avian egg surface with the peracetic acid solution, the avian egg surface is appropriately dried according to general hygiene standards. Examples of drying include natural drying and drying with a clean blower. Peracetic acid is removed from the avian egg surface by drying the avian egg surface.

As described above, the waste solution of the peracetic acid solution used for sterilizing the surface of the bird egg can maintain the effect of the peracetic acid for a long period of time, especially in the case of the peracetic acid solution produced by the equilibrium reaction. It also contributes to the sterilization of the floor surface in the factory and the drainage ditch in the facility. Ultimately, it is mixed and neutralized with wastewater discharged for other purposes, so there is less impact on sewage purification microorganisms in the treatment tank and problems with wastewater standards.
However, if a large amount of high-concentration peracetic acid solution is discharged during periodic inspections, etc., a peracetic acid neutralizer such as sodium metabisulfite may be used to protect the sewage purification microorganisms in the wastewater treatment tank. preferable.

The bird egg is not particularly limited, and examples thereof include chicken eggs, quail eggs, and duck eggs. The contact time between the surface of the bird egg and the peracetic acid solution can be appropriately changed depending on the size of the bird egg.

According to the method for sterilizing the surface of a bird egg of the present invention, it is possible to sterilize and remove the bacteria adhering to the surface of the bird egg and the bacteria sneaking into the cuticle layer existing on the surface of the bird egg. The cuticle layer on the surface of the bird egg can be removed by a stream of water or by washing with a brush, but the method of the present invention can improve the removal rate of bacteria.

Hereinafter, the present invention will be described in more detail with reference to specific examples. However, the present invention is not limited to the examples shown below.

[Example 1]
When the contamination status of 100 unwashed chicken eggs was examined by the total number of bacteria, the distribution was as shown in Table 1. The minimum value (log) was 0.57 / ml, the maximum value (log) was 7.42 / ml, and it was found that the maximum value was around 5 in the log value. The graph of Table 1 is shown in FIG.

Table 2 shows the types and numbers of bacteria detected on the surface of chicken eggs shown in Table 1. From Table 2, it can be seen that 3 to 5 bacteria are attached to the surface of the eggs laid on the log scale.

Next, the bactericidal effect of the peracetic acid solution on the surface of bird eggs was verified. Each of the five chicken eggs whose bacterial counts were measured in advance was immersed in peracetic acid solutions having different concentrations, and the bacterial count after immersion was measured. The results are shown in Table 3. The peracetic acid solution was produced by mixing glacial acetic acid and hydrogen peroxide.

As shown in Table 3, by immersing the chicken egg in a peracetic acid solution, bacteria existing on the surface of the chicken egg could be sterilized and removed. As for the bactericidal effect on the surface of chicken eggs by the peracetic acid solution, a sufficient effect was obtained by soaking for only 1 minute when the peracetic acid concentration was 350 ppm or more.

[Example 2]
Instead of immersing the surface of the egg in Example 1 in the peracetic acid solution, a 500 ppm peracetic acid solution was sprayed from above onto the egg moving on the conveyor belt. In this case, the moving speed of the chicken egg was 30 cm per minute, and the spraying distance was 1 m. In this case, the peracetic acid solution is sprayed for 3 minutes or more. By spraying this peracetic acid solution, the number of bacteria detected on the surface of the chicken egg after spraying became zero.

[Example 3]
Five of the 100 unwashed chicken eggs of Example 1 were randomly selected, the non-woven fabric was impregnated with a peracetic acid solution having a concentration of 350 ppm, and then the surface of the chicken eggs was wiped in a sufficiently squeezed state. It was carried out for 3 minutes per chicken egg. After that, as a result of measuring the number of bacteria on the surface of the chicken egg, the number of detected bacteria was 0.

INDUSTRIAL APPLICABILITY The present invention provides a method for sterilizing a bird egg surface, which comprises contacting a bird egg surface with a peracetic acid solution. In Japan, bird eggs are often eaten raw. Therefore, it is necessary that the bird eggs are completely sterilized. Since bird eggs are produced from the cloaca, they are contaminated with intestinal bacteria. Some of these bacteria are addictive and dangerous. The method for sterilizing the surface of a bird egg of the present invention can efficiently sterilize the surface of a bird egg in a short time. In addition, the method for sterilizing the surface of bird eggs of the present invention has few problems with drainage standards.

1 ... Spray nozzle 2 ... Rotating brush 3 ... Belt conveyor 4 ... Bird egg 10 ... Peracetic acid solution spray device

Claims (8)

A method for sterilizing a bird egg surface, which comprises contacting the bird egg surface with a peracetic acid solution. The method according to claim 1, wherein the concentration of peracetic acid is 80 to 1000 ppm. The method according to claim 1 or 2, wherein the surface of the bird egg and the peracetic acid solution are brought into contact with each other at 10 to 40 ° C. for 5 seconds to 20 minutes. The method according to any one of claims 1 to 3, wherein the peracetic acid solution is an equilibrium peracetic acid solution composed of glacial acetic acid and hydrogen peroxide. The method according to any one of claims 1 to 4, wherein the contact between the bird egg surface and the peracetic acid solution is by spraying the peracetic acid solution onto the bird egg surface. The method according to any one of claims 1 to 5, wherein the contact between the bird egg surface and the peracetic acid solution is performed by stroking the bird egg surface with a non-woven fabric containing the peracetic acid solution. The method according to any one of claims 1 to 6, wherein the bird egg is an unfertilized egg or a fertilized egg. The method according to any one of claims 1 to 7, wherein the bird egg is a chicken egg, a quail egg, or a duck egg.

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