JPWO2016047570A1 - Deep-fried food taste enhancer - Google Patents

Deep-fried food taste enhancer Download PDF

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JPWO2016047570A1
JPWO2016047570A1 JP2016550160A JP2016550160A JPWO2016047570A1 JP WO2016047570 A1 JPWO2016047570 A1 JP WO2016047570A1 JP 2016550160 A JP2016550160 A JP 2016550160A JP 2016550160 A JP2016550160 A JP 2016550160A JP WO2016047570 A1 JPWO2016047570 A1 JP WO2016047570A1
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JP6630279B2 (en
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前田 竜郎
竜郎 前田
葉 廣瀬
葉 廣瀬
健二郎 下司
健二郎 下司
辰徳 西出
辰徳 西出
祐二 田上
祐二 田上
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Nisshin Foods Inc
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Abstract

本発明の揚げ物の呈味増強剤は、タウリン及び、動物性又は植物性蛋白の抽出物由来の分子量80万〜1万のペプチドの混合物を含有する。本発明の揚げ物の製造方法は、具材100質量部に対して、タウリン0.001〜0.5質量部、及び動物性又は植物性蛋白の抽出物由来の分子量が80万〜1万のペプチドの混合物0.001〜0.5質量部を付着させた後、油ちょうする。呈味増強剤は、例えば、揚げ物用の調味料、打ち粉又は衣材等として用いられる。The deep-fried food taste enhancer of the present invention contains a mixture of taurine and a peptide having a molecular weight of 800,000 to 10,000 derived from an extract of animal or vegetable protein. The method for producing fried food according to the present invention is a peptide having 0.001 to 0.5 parts by mass of taurine and a molecular weight derived from an animal or vegetable protein extract of 800,000 to 10,000 with respect to 100 parts by mass of ingredients. After adhering 0.001 to 0.5 parts by mass of the mixture, oil is added. The taste enhancer is used, for example, as a seasoning for deep-fried food, a dusting powder, or a clothing material.

Description

本発明は、揚げ物の旨味や味の深みを増大させることができる、揚げ物の呈味増強剤及び揚げ物の製造方法に関する。   TECHNICAL FIELD The present invention relates to a deep-fried food taste enhancer and a method for producing a deep-fried food, which can increase the umami and depth of the deep-fried food.

揚げ物は、高温の油で加熱する調理法であり、加熱と同時に、食材表面の水分が油に置換されるため、食材の風味と油の風味と高温による焦げの風味が相俟って、独特の味わいが得られる食品である。他方、揚げ物は、調理後時間が経過したり、調理の仕方によっては油感の高いものができてしまい、風味に厚み、濃厚感が足りなくなったり、しつこくて食べにくくなったりすることがある。
そのため、食材の風味を活かし、揚げ物独特の風味も維持しながら、深い味わいを有する揚げ物が求められている。
Deep-fried food is a cooking method that heats with high-temperature oil. At the same time as heating, the moisture on the surface of the food is replaced with oil, so the flavor of the food, the flavor of the oil, and the scorching flavor due to the high temperature are combined. It is a food product that can be enjoyed. On the other hand, deep-fried foods may have a high oily feeling depending on how they are cooked, depending on how they are cooked, resulting in lack of flavor and thickness, and may be persistent and difficult to eat.
Therefore, there is a demand for deep-fried fried foods that utilize the flavor of ingredients and maintain the unique flavor of fried foods.

特許文献1〜3には、カルノシンやアンセリンのようなイミダゾールジペプチド、タウリンのようなスルホン基含有化合物を用いるコク味付与剤が開示されている。また、特許文献4には、分子量1万以上のペプチド分画の比率が10%以上で、分子量2000以下の分画が大半を占める酵母分解エキスが、畜肉又は魚介類を煮出ししただし液について、だしの呈味を強化することが開示されている。また、特許文献5には、肉質蛋白質を分解した調味料が開示されている。しかしながら、これらの成分によっても、揚げ物特有の風味にコクのある厚み、濃厚感を与え、しつこさのない揚げ物を得るには不十分であった。   Patent Documents 1 to 3 disclose body taste imparting agents using imidazole dipeptides such as carnosine and anserine and sulfone group-containing compounds such as taurine. Further, in Patent Document 4, a yeast-degrading extract in which a fraction of a peptide fraction having a molecular weight of 10,000 or more is 10% or more, and a fraction having a molecular weight of 2000 or less occupies most of the meat or seafood, except for the liquid, It is disclosed to enhance the taste of dashi. Patent Document 5 discloses a seasoning obtained by decomposing meat protein. However, these ingredients are not sufficient to give a rich and rich feeling to the characteristic flavor of the deep-fried food, and to obtain a deep-fried food with no persistence.

特開平7−203895号公報JP-A-7-203895 特開平7−265008号公報JP 7-265008 A 特開平8−289760号公報JP-A-8-289760 特開2005−102549号公報JP 2005-102549 A US2007077333(A1)US2007077333 (A1)

本発明は、食材の風味を活かし、揚げ物独特の風味も維持しながら、深い味わいを有する揚げ物を容易に得ることができる呈味増強剤等を提供することを課題とする。   This invention makes it a subject to provide the flavor enhancer etc. which can obtain the deep-fried food easily, utilizing the flavor of foodstuffs and maintaining the characteristic flavor of deep-fried food.

上記の課題を達成するために、本発明者らは、種々の成分を揚げ物に適用し検討を重ねた。その結果、タウリンと動物性又は植物性蛋白の抽出物由来の特定の分子量のペプチドとの混合物が、揚げ物の風味に深みを与え、これを用いると味わい深い揚げ物食品が簡便に得られることを見出し、本発明を完成した。   In order to achieve the above-mentioned problems, the present inventors have applied various components to deep-fried foods and repeated studies. As a result, it has been found that a mixture of taurine and a peptide having a specific molecular weight derived from an animal or vegetable protein extract gives depth to the flavor of fried food, and when this is used, a tasty fried food can be easily obtained, The present invention has been completed.

即ち本発明は、タウリン及び、動物性又は植物性蛋白の抽出物由来の分子量80万〜1万のペプチドの混合物を含有する揚げ物の呈味増強剤を提供する。
また本発明は、具材100質量部に対して、タウリン0.001〜0.5質量部、及び動物性又は植物性蛋白の抽出物由来の分子量が80万〜1万のペプチドの混合物0.001〜0.5質量部を付着させた後、油ちょうする揚げ物の製造方法を提供する。
That is, the present invention provides a fried food taste enhancer containing a mixture of taurine and a peptide having a molecular weight of 800,000 to 10,000 derived from an animal or vegetable protein extract.
The present invention also relates to a mixture of 0.001 to 0.5 parts by mass of taurine and a peptide having a molecular weight of 800,000 to 10,000 derived from animal or vegetable protein extracts, based on 100 parts by mass of ingredients. After attaching 001-0.5 mass part, the manufacturing method of the fried food which oils is provided.

図1は、鶏胸肉から得た抽出物のHPLCチャートを示す図である。FIG. 1 is an HPLC chart of an extract obtained from chicken breast. 図2は、鶏腿肉から得た抽出物のHPLCチャートを示す図である。FIG. 2 is a diagram showing an HPLC chart of an extract obtained from chicken thigh.

本発明の呈味増強剤及び揚げ物の製造方法で用いるタウリンは、牛の胆汁中から発見された成分であり、貝類、イカ、タコの肉や神経組織等に含まれている。タウリンは生物から抽出したものでも、合成したものでも使用することができるが、好ましくは抽出して得られたものである。タウリンは、市販品を利用してもよい。
本発明の呈味増強剤におけるタウリンは、その使用量が、揚げ物の具材100質量部に対して、好ましくは0.001〜0.5質量部、より好ましくは0.01〜0.3質量部になるよう配合する。例えば、具材100gに呈味増強剤10gを打ち粉として付着させ、揚げ物を製造する場合、呈味増強剤10g中、タウリンの配合量は好ましくは0.001〜0.5gであり、打ち粉である呈味増強剤100質量%中のタウリンの配合量(濃度)は好ましくは0.01〜5質量%である。また、呈味増強剤が、調味料や衣材である場合も同様に、呈味増強剤100質量%中のタウリンの配合量(濃度)は好ましくは0.01〜5質量%である。
本発明の揚げ物の製造方法においては、揚げ物の具材100質量部に対して、タウリンを0.001〜0.5質量部、好ましくは0.01〜0.3質量部付着させる。
Taurine used in the method for producing a taste enhancer and fried food according to the present invention is a component found in bile of cattle and is contained in shellfish, squid, octopus meat, nerve tissue, and the like. Taurine may be extracted from organisms or synthesized, but is preferably obtained by extraction. A commercially available product may be used for taurine.
The amount of taurine in the taste enhancer of the present invention is preferably 0.001 to 0.5 parts by mass, more preferably 0.01 to 0.3 parts by mass, based on 100 parts by mass of the ingredients of the deep-fried food. Blend to be part. For example, when 10 g of the taste enhancer is attached to 100 g of ingredients as a dust and a fried product is produced, the amount of taurine in the taste enhancer 10 g is preferably 0.001 to 0.5 g. The blending amount (concentration) of taurine in 100% by mass of the taste enhancer is preferably 0.01 to 5% by mass. Similarly, when the taste enhancer is a seasoning or a clothing material, the amount (concentration) of taurine in 100% by mass of the taste enhancer is preferably 0.01 to 5% by mass.
In the manufacturing method of the fried food of this invention, 0.001-0.5 mass part, Preferably 0.01-0.3 mass part of taurine is made to adhere with respect to 100 mass parts of the ingredients of fried food.

本発明の呈味増強剤及び揚げ物の製造方法で用いる動物性又は植物性蛋白の抽出物(抽出エキス)由来のペプチドは、牛、豚、鶏等の畜肉類;鰯、鮭等の魚類;イカ、タコ等の軟体動物類等の動物性蛋白から抽出して得られたペプチド、又は、大豆、麦、米等の穀類;ジャガイモ等のイモ類;ニンジン、ゴボウ等の根菜類等の植物性蛋白から抽出して得られたペプチドの内、ゲル濾過による分子量が80万〜1万ダルトンのペプチドである。動物性又は植物性蛋白由来であっても、加水分解を行ったものや、ゲル濾過による分子量が1万ダルトン未満のペプチドであると、ペプチド特有の苦味が強くなるため、本発明の効果は得られない。また、ゲル濾過による分子量が80万ダルトンを超えると、味が感じられにくくなる上、調製がしづらくなるため、コスト的にも不利になる。   Peptides derived from animal or vegetable protein extracts (extract extracts) used in the method for producing a taste enhancer and fried food of the present invention include cattle, pigs, chickens and other livestock meats; Peptides obtained by extracting from animal proteins such as mollusks such as octopus, octopus, etc., or grains such as soybeans, wheat and rice; potatoes such as potato; plant proteins such as root vegetables such as carrot and burdock Among the peptides obtained by extraction from the above, it is a peptide having a molecular weight of 800,000 to 10,000 daltons by gel filtration. Even if it is derived from animal or vegetable protein, the effect of the present invention can be obtained because the bitterness peculiar to the peptide becomes strong if it is hydrolyzed or if the molecular weight by gel filtration is less than 10,000 daltons. I can't. Moreover, when the molecular weight by gel filtration exceeds 800,000 daltons, it becomes difficult to feel the taste and it is difficult to prepare, which is disadvantageous in terms of cost.

上記動物性又は植物性蛋白としては、動物又は植物における、蛋白質を多く含む部位を用いることが好ましく、例えば、動物性蛋白としては、筋肉、表皮等を、植物性蛋白としては、種、実等を用いることが好ましい。特に動物性蛋白が好ましく、筋肉繊維等の繊維状蛋白が最も好ましい。
原料となる動物性又は植物性蛋白からの抽出には、アルコール又は酸を用いて抽出する、公知の蛋白抽出方法を用いることができる。例えば、原料を細断、破砕等の方法で細分化し、50〜90%エタノール等のアルコール溶液、又は、10〜500mM酢酸等の酸溶液を用いて抽出することができる。こうして得られた蛋白抽出物から、目的物である分子量80万〜1万のペプチドを得る。また、動物性蛋白又は植物性蛋白としては、それぞれ、単一種類の動物又は植物のものを用いても良いし、複数種類の動物又は植物のものを組み合わせて用いても良い。また、動物性蛋白と植物性蛋白とを組み合わせて原料とすることもできる。
As the animal or plant protein, it is preferable to use a protein-rich site in animals or plants. For example, as an animal protein, muscle, epidermis, etc., as a plant protein, species, fruit, etc. Is preferably used. Animal protein is particularly preferable, and fibrous protein such as muscle fiber is most preferable.
For extraction from animal or vegetable protein as a raw material, a known protein extraction method using alcohol or acid can be used. For example, the raw material can be fragmented by a method such as shredding or crushing, and extracted using an alcohol solution such as 50 to 90% ethanol or an acid solution such as 10 to 500 mM acetic acid. From the protein extract thus obtained, a target peptide having a molecular weight of 800,000 to 10,000 is obtained. Moreover, as animal protein or vegetable protein, you may use the thing of a single kind of animal or a plant, respectively, and you may use the thing of a plurality of kinds of animals or plants in combination. Moreover, animal protein and vegetable protein can also be used as a raw material in combination.

目的とする分子量のペプチドを得る方法は、ゲル濾過、限外濾過、遠心分離等の公知の方法によって、上記蛋白抽出物から分取する方法を用いることができるが、本発明における「ペプチドの混合物」には、分子量が80万〜1万(1万以上80万以下)のペプチドに加え、分取操作上、不可避的に混入する分子量80万超、1万未満の分画を含むことも、本願の効果を妨げない限り許容される。
本発明における「ペプチドの混合物」は、動物性又は植物性蛋白からの抽出物に由来するものであり、分子量が相互に異なるペプチドを含む混合物である。より好ましくは、ゲル濾過により分子量の分布を分析したときに、分子量が1万以上80万以下の範囲に複数のピークを有する混合物である。
「ペプチドの混合物」を構成するペプチドの分子量は、好ましくは50万〜5万、更に好ましくは50万〜10万である。
As a method for obtaining a peptide having a target molecular weight, a method of fractionating the protein extract by a known method such as gel filtration, ultrafiltration, or centrifugation can be used. In addition to a peptide having a molecular weight of 800,000 to 10,000 (10,000 to 800,000), it also includes a fraction having a molecular weight of more than 800,000 and less than 10,000, which is inevitably mixed in the fractionation operation. It is permissible as long as the effect of the present application is not disturbed.
The “mixture of peptides” in the present invention is derived from extracts from animal or plant proteins, and is a mixture containing peptides having different molecular weights. More preferably, the molecular weight distribution is a mixture having a plurality of peaks in the range of 10,000 to 800,000 when the molecular weight distribution is analyzed by gel filtration.
The molecular weight of the peptide constituting the “mixture of peptides” is preferably 500,000 to 50,000, more preferably 500,000 to 100,000.

本発明における「ペプチドの混合物」は、ゲル濾過法により決定される分子量において、分子量が80万〜1万のペプチドの割合が、全ペプチドの合計質量に対して80〜100質量%であることが好ましく、より好ましくは90〜100質量%、更に好ましくは100質量%である。同様に、本発明における「ペプチドの混合物」は、ゲル濾過法により決定される分子量において、分子量が50万〜5万(又は50万〜10万)のペプチドの割合が、全ペプチドの合計質量に対して80〜100質量%であることが好ましく、より好ましくは90〜100質量%、更に好ましくは100質量%である。
分子量を分析するためのゲル濾過HPLCの具体的な条件は実施例において後述する。
In the molecular weight determined by the gel filtration method, the “mixture of peptides” in the present invention is such that the proportion of peptides having a molecular weight of 800,000 to 10,000 is 80 to 100% by mass relative to the total mass of all peptides. More preferably, it is 90-100 mass%, More preferably, it is 100 mass%. Similarly, in the “peptide mixture” in the present invention, the proportion of peptides having a molecular weight of 500,000 to 50,000 (or 500,000 to 100,000) in the molecular weight determined by gel filtration is the total mass of all peptides. It is preferable that it is 80-100 mass% with respect to it, More preferably, it is 90-100 mass%, More preferably, it is 100 mass%.
Specific conditions of gel filtration HPLC for analyzing the molecular weight will be described later in Examples.

本発明の呈味増強剤におけるペプチドは、その使用量が、揚げ物の具材100質量部に対して、好ましくは0.001〜0.5質量部、より好ましくは0.01〜0.2質量部になるよう配合する。例えば、具材100gに呈味増強剤10gを打ち粉として付着させ、揚げ物を製造する場合、呈味増強剤10g中、ペプチドの配合量は好ましくは0.001〜0.5gであり、打ち粉である呈味増強剤100質量%中のペプチドの配合量(濃度)は好ましくは0.01〜5質量%である。
本発明の揚げ物の製造方法においては、揚げ物の具材100質量部に対して、ペプチドを0.001〜0.5質量部、好ましくは0.01〜0.2質量部付着させる。
The amount of the peptide used in the taste enhancer of the present invention is preferably 0.001 to 0.5 parts by mass, more preferably 0.01 to 0.2 parts by mass, with respect to 100 parts by mass of the ingredients of the deep-fried food. Blend to be part. For example, when 10 g of the taste enhancer is attached to 100 g of ingredients as a dust and a fried food is produced, the amount of peptide in the taste enhancer 10 g is preferably 0.001 to 0.5 g. The blending amount (concentration) of the peptide in 100% by mass of the taste enhancer is preferably 0.01 to 5% by mass.
In the manufacturing method of the deep-fried food of this invention, 0.001-0.5 mass part, preferably 0.01-0.2 mass part of peptides are made to adhere with respect to 100 mass parts of the ingredients of deep-fried food.

本発明の呈味増強剤におけるタウリンとペプチドの配合割合(質量比,前者:後者)、及び、本発明の揚げ物の製造方法によけるタウリンとペプチドとの使用割合(質量比,前者:後者)は、それぞれ、100:1〜1:100であることが好ましく、より好ましくは10:1〜1:10である。   The proportion of taurine and peptide in the taste enhancer of the present invention (mass ratio, former: latter) and the proportion of taurine and peptide used in the method for producing fried food of the present invention (mass ratio, former: latter) Are preferably 100: 1 to 1: 100, more preferably 10: 1 to 1:10.

本発明の呈味増強剤は、上記の成分に加えて、酸性アミノ酸、炭化水素アミノ酸、グリシン及びこれらの塩から選択される1種以上を配合すると、更に深い味わいが得られるようになるため好ましい。酸性アミノ酸は、側鎖にカルボキシル基等のプロトンを放出する基を有するアミノ酸であり、アスパラギン酸、グルタミン酸などが挙げられる。炭化水素アミノ酸は、側鎖に炭化水素基を有するアミノ酸であり、アラニン、バリン、ロイシン、イソロイシンなどが挙げられる。これらのアミノ酸(酸性アミノ酸、炭化水素アミノ酸、グリシン、又はそれらの塩)は、一種を単独で用いても良いし、2種以上を併用することもできる。以上の中でも、アスパラギン酸、グルタミン酸、アラニン、グリシンおよびその塩から選択される1種以上を配合するのが好ましい。   The taste enhancer of the present invention is preferable because, in addition to the above components, when one or more selected from acidic amino acids, hydrocarbon amino acids, glycine, and salts thereof are blended, a deeper taste can be obtained. . An acidic amino acid is an amino acid having a group that releases a proton such as a carboxyl group in the side chain, and examples include aspartic acid and glutamic acid. The hydrocarbon amino acid is an amino acid having a hydrocarbon group in the side chain, and examples thereof include alanine, valine, leucine, and isoleucine. These amino acids (acidic amino acids, hydrocarbon amino acids, glycine, or salts thereof) may be used alone or in combination of two or more. Among these, it is preferable to blend one or more selected from aspartic acid, glutamic acid, alanine, glycine and salts thereof.

本発明の呈味増強剤における上記アミノ酸は、その使用量が、揚げ物の具材100質量部に対して、好ましくは0.001〜0.5質量部、より好ましくは0.01〜0.2質量部となるように配合する。例えば、具材100gに呈味増強剤10gを打ち粉として付着させ、揚げ物を製造する場合、打ち粉である呈味増強剤10g中、アミノ酸の配合量は好ましくは0.001〜0.5gであり、打ち粉である呈味増強剤100質量%中のアミノ酸の配合量(濃度)は好ましくは0.01〜5質量%である。また、本発明の揚げ物の製造方法においては、揚げ物の具材100質量部に対して、アミノ酸ペプチドを0.001〜0.5質量部、好ましくは0.01〜0.2質量部付着させる。
本発明の呈味増強剤におけるタウリンとアミノ酸の配合割合(質量比,前者:後者)、及び、本発明の揚げ物の製造方法によけるタウリンとアミノ酸との使用割合(質量比,前者:後者)は、それぞれ、100:1〜1:100であることが好ましく、より好ましくは10:1〜1:10である。
The amount of the amino acid used in the taste enhancer of the present invention is preferably 0.001 to 0.5 parts by mass, and more preferably 0.01 to 0.2, with respect to 100 parts by mass of the ingredients of the deep-fried food. It mix | blends so that it may become a mass part. For example, in the case of producing fried food by attaching 10 g of a taste enhancer as a flour to 100 g of ingredients, the amount of amino acids in the taste enhancer 10 g that is a flour is preferably 0.001 to 0.5 g. Yes, the blending amount (concentration) of the amino acid in 100% by mass of the taste enhancer which is a dusting powder is preferably 0.01 to 5% by mass. Moreover, in the manufacturing method of the fried food of this invention, 0.001-0.5 mass part, Preferably 0.01-0.2 mass part of amino acid peptides are made to adhere with respect to 100 mass parts of the ingredients of fried food.
The proportion of taurine and amino acid in the taste enhancer of the present invention (mass ratio, former: latter) and the proportion of taurine and amino acid used in the method for producing fried food of the present invention (mass ratio, former: latter) Are preferably 100: 1 to 1: 100, more preferably 10: 1 to 1:10.

本発明の呈味増強剤は、前述したタウリン、ペプチド、アミノ酸に加えて、呈味増強剤を揚げ物食品に適用する形態に合わせて、適宜公知のその他成分を配合することができる。   In addition to the above-described taurine, peptide, and amino acid, the taste enhancer of the present invention can be appropriately mixed with other known components according to the form in which the taste enhancer is applied to fried food.

本発明の呈味増強剤を揚げ物用の調味料として用いる場合、水;油脂;酒;みりん;醤油、塩;砂糖;香辛料等、公知の調味料に配合される成分を配合して製造できる。本発明の呈味増強剤を揚げ物用の打ち粉として用いる場合、小麦粉等の穀粉;澱粉;糖類等、公知の打ち粉に配合される成分を配合して製造できる。本発明の呈味増強剤を揚げ物用の衣材として用いる場合、小麦粉等の穀粉;澱粉;糖類;卵粉;増粘剤;食塩、粉末醤油、発酵調味料やその他の調味料;香辛料;香料;ビタミン等の栄養成分;着色料;油脂;膨張剤等、公知の衣材に配合される成分を配合して製造できる。その他成分の配合量は、上記の本発明の呈味増強剤中のタウリン、ペプチド、アミノ酸量を配合できるように調整すればよいが、一般的には呈味増強剤100質量%中95〜99.9質量%程度である。   When the flavor enhancer of the present invention is used as a seasoning for deep-fried foods, it can be produced by blending ingredients such as water; fats and oils; sake; mirin; soy sauce, salt; sugar; When the taste enhancer of the present invention is used as a flour for fried foods, it can be produced by blending ingredients such as flour such as wheat flour; starch; When using the flavor enhancer of the present invention as a fried food material, flour such as flour; starch; sugar; egg powder; thickener; salt, powdered soy sauce, fermented seasoning and other seasonings; spices; Nutritional components such as vitamins; coloring agents; fats and oils; swelling agents and other components blended in known clothing materials can be blended to produce. The amount of other components may be adjusted so that the amount of taurine, peptide, and amino acid in the above-described taste enhancer of the present invention can be blended, but generally 95 to 99 in 100% by mass of the taste enhancer. About 9% by mass.

本発明の呈味増強剤を用いて揚げ物を製造する場合、上記の呈味増強剤の形態に応じて、通常の製造方法により行えばよい。例えば、揚げ物用の調味料の呈味増強剤を用いる場合、呈味増強剤をボウル等に張り、これに具材をくぐらせる等して付着させ、そのままか衣材を付着させて油ちょうする。また、揚げ物用の打ち粉の呈味増強剤を用いる場合、打ち粉を具材にまぶして付着させ、そのままか更に衣材を付着させて油ちょうする。また、揚げ物用の衣材の呈味増強剤を用いる場合、具材をそのままか必要に応じて調味液や打ち粉を付着させた後、衣材をまぶして付着させるか、衣液を水に溶かしたバッター液を付着させ、油ちょうすればよい。
揚げ物の具材としては、揚げ物とされる公知の各種の具材を特に制限なく用いることができ、例えば、牛、豚、鶏等の畜肉類、キス、エビ、イカ、タコ等の魚介類、イモ、ナス、カボチャ、ニンジン、タマネギ、キノコ類等の野菜類等が挙げられる。
What is necessary is just to perform by a normal manufacturing method, when manufacturing a fried food using the taste enhancer of this invention according to the form of said taste enhancer. For example, when using a seasoning enhancer for fried foods, apply the seasoning enhancer to a bowl or the like and attach it by passing ingredients, etc. . In addition, when using a flour-enhancing taste enhancer for fried foods, the dusting powder is applied to the ingredients and adhered, and then the clothes are adhered as it is and oiled. In addition, when using a flavor enhancer for fried foods, add the seasoning liquid or dusting powder as it is or if necessary, and then sprinkle the clothes or attach the liquid to the water. The melted batter liquid may be attached and oiled.
As the ingredients of the fried food, known various ingredients that are considered to be deep-fried can be used without particular limitation, for example, livestock such as cows, pigs, chickens, fish and shellfish such as kisses, shrimps, squids, octopus, Vegetables such as tubers, eggplants, pumpkins, carrots, onions, mushrooms and the like.

本発明の呈味増強剤は、揚げ物用の公知の調味料、打ち粉又は衣材等に添加して用いる添加剤であっても良い。添加剤である呈味増強剤は、タウリン、分子量80万〜1万のペプチドの混合物のみからなるものや、タウリンと分子量80万〜1万のペプチドの混合物に更に前記のアミノ酸を配合したものであっても良い。また、本発明の揚げ物の製造方法においては、具材に対して、タウリンと分子量80万〜1万のペプチドの混合物と所望により配合する前記のアミノ酸を任意の順序で順次付着させても良いし、それらを含む混合物等として同時に付着させても良い。油ちょうは、油の温度を130℃以上に加熱して行うことが好ましく、より好ましくは160℃〜200℃に加熱して行う。   The taste enhancer of the present invention may be an additive used by adding to a known seasoning for fried foods, dusting or clothing. The taste enhancer, which is an additive, is composed of only taurine, a mixture of peptides having a molecular weight of 800,000 to 10,000, or a mixture of taurine and a peptide having a molecular weight of 800,000 to 10,000. There may be. In the fried food production method of the present invention, a mixture of taurine and a peptide having a molecular weight of 800,000 to 10,000 and the above-mentioned amino acids to be blended as desired may be sequentially attached to the ingredients in any order. Alternatively, they may be attached simultaneously as a mixture containing them. The oil is preferably heated by heating the oil to 130 ° C. or higher, more preferably 160 ° C. to 200 ° C.

以下、実施例を挙げて、本発明を更に詳細に説明するが、本発明は以下の実施例より何ら限定されるものではない。   EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further in detail, this invention is not limited at all from a following example.

(参考例1〜3)鶏由来のペプチドの製造
鶏胸肉100gをハサミで3mm角程度に細断し、更にホモジナイザーを用いて粉砕した。これに100mLの70%エタノールを加え、60分間振盪(60rpm)して抽出を行った。抽出操作後、0.45μm のフィルターで濾過して濾液を収集した。
この濾液のゲル濾過HPLCのチャートを図1に示す。このチャートの分子量80万〜1万の範囲を分取して参考例1とし、50万〜5万の範囲を分取して参考例2とし、80万超の範囲を分取して参考例3とした。
(Reference Examples 1-3) Production of chicken-derived peptide 100 g of chicken breast was chopped to about 3 mm square with scissors, and further pulverized using a homogenizer. To this was added 100 mL of 70% ethanol, and extraction was performed by shaking (60 rpm) for 60 minutes. After the extraction operation, the filtrate was collected by filtration through a 0.45 μm filter.
A chart of gel filtration HPLC of this filtrate is shown in FIG. In this chart, the molecular weight range of 800,000 to 10,000 is taken as reference example 1, the 500,000 to 50,000 range is taken as reference example 2, and the range of more than 800,000 is taken as a reference example. It was set to 3.

上記のゲル濾過HPLCは下記条件にて行った。
カラムとしてTSK-GEL G3000SWXL+TSK-GEL G2000SWXL (東ソー社製)を使用した。移動相は、0.3MNaClを含むリン酸緩衝液20mM(pH7.0)を用いた。測定は25℃で行ない、流速0.5mL/分、検出器はDAD(ダイオードアレイ検出器)を用い280nmにおける吸光度を検出した。
また、参考例1〜3のペプチドの分取は、同条件のゲル濾過HPLCにおいて分子量マーカーにより作成した検量線から分子量画分を計算し、フラクションコレクターを用いて行った。各分子量画分のフラクションを回収した後、凍結乾燥機を用いて粉末にした。タンパク質の分子量マーカーとして、オリエンタル酵母工業(株)社製のグルタミン酸脱水素酵素(MW:290,000)、乳酸脱水素酵素(MW:142,000)、エノラーゼ(MW:67,000)、ミオキラーゼ(MW:32,000)、シトクロムC(MW:12,400)を用いた。
The gel filtration HPLC was performed under the following conditions.
As the column, TSK-GEL G3000SWXL + TSK-GEL G2000SWXL (manufactured by Tosoh Corporation) was used. As the mobile phase, a phosphate buffer solution 20 mM (pH 7.0) containing 0.3 M NaCl was used. The measurement was performed at 25 ° C., the flow rate was 0.5 mL / min, and the detector used DAD (diode array detector) to detect the absorbance at 280 nm.
Moreover, fractionation of the peptides of Reference Examples 1 to 3 was carried out using a fraction collector by calculating a molecular weight fraction from a calibration curve prepared with molecular weight markers in gel filtration HPLC under the same conditions. The fraction of each molecular weight fraction was collected and then powdered using a freeze dryer. Protein molecular weight markers include glutamate dehydrogenase (MW: 290,000), lactate dehydrogenase (MW: 142,000), enolase (MW: 67,000), myoxase (MW: 32,000), cytochrome, manufactured by Oriental Yeast Co., Ltd. C (MW: 12,400) was used.

(参考例4〜6)鶏由来のペプチドの製造
参考例1と同様に、鶏腿肉200gを用いて抽出を行い、濾液を得た。この抽出物のゲル濾過HPLCのチャートを図2に示す。このチャートの分子量80万〜1万の範囲を分取して参考例4とし、50万〜10万の範囲を分取して参考例5とし、1万未満の範囲を分取して参考例6とした。
(Reference Examples 4-6) Production of chicken-derived peptide In the same manner as in Reference Example 1, extraction was performed using 200 g of chicken thigh, and a filtrate was obtained. A gel filtration HPLC chart of this extract is shown in FIG. In this chart, the molecular weight range of 800,000 to 10,000 is taken as reference example 4, the 500,000 to 100,000 range is taken as reference example 5, and the range less than 10,000 is taken as a reference example. It was set to 6.

(製造例1〜12)
表1に示す成分を均一に混合して、製造例1〜12のから揚げ用衣材をそれぞれ製造した。製造例1〜12の揚げ用衣材は、製造例1がタウリン、製造例7が分子量80万〜1万のペプチドを含有しない点で比較例であり、それら以外は実施例である。
(Production Examples 1-12)
The ingredients shown in Table 1 were mixed uniformly to produce the fried dressing materials of Production Examples 1 to 12, respectively. The fried dressings of Production Examples 1 to 12 are comparative examples in that Production Example 1 does not contain taurine and Production Example 7 does not contain a peptide having a molecular weight of 800,000 to 10,000, and the others are examples.

(評価試験1)
鶏腿肉の細片(25g/個)を20個ずつ用意し、製造例1〜12の各衣材を、肉100gあたりの衣材の付着量が10gになるよう計量して、肉全体にまぶしてよく付着させた。衣材の付着した鶏腿肉を、170℃に熱したサラダ油で4分間油ちょうして、鶏肉のから揚げを製造した。揚げたてのから揚げ(油からあげて5分後)、及び室温(約25℃)に1時間放置したから揚げの風味を、それぞれ10名のパネラーに、表2に示す評価基準で評価してもらった。その平均点を表1に示す。
(Evaluation Test 1)
Prepare 20 pieces of chicken thigh flakes (25g / piece) and weigh each clothing material of Production Examples 1 to 12 so that the amount of clothing material applied per 100g of meat is 10g. It was well adhered by dusting. The chicken thighs to which the clothing material was attached were oiled with salad oil heated to 170 ° C. for 4 minutes to produce fried chicken. Freshly fried (after 5 minutes from oil) and left at room temperature (about 25 ° C) for 1 hour, so the flavor of fried food was evaluated by 10 panelists according to the evaluation criteria shown in Table 2 . The average score is shown in Table 1.

Figure 2016047570
Figure 2016047570

Figure 2016047570
Figure 2016047570

(製造例13〜20)
下記表3に示す配合で、製造例13〜20の揚げ物用衣材を製造した。製造例13〜20の揚げ用衣材は、製造例17〜20の揚げ用衣材が、製造例17〜19に含まれるペプチドの分子量が80万超である点及び製造例20に含まれるペプチドの分子量が1万未満である点で、それぞれ比較例であり、それら以外は実施例である。
(評価試験2)
各揚げ物用衣材100質量部に対して、水200質量部を混合してバッター液を製造した。打ち粉をした豚ロース肉100gに、このバッター液を絡め、更にパン粉を付着させ、170℃に熱したサラダ油で6分間油ちょうして、とんかつを製造した。
揚げたてのとんかつ(油からあげて5分後)、および室温(約25℃)に1時間放置したとんかつの風味を、それぞれ10名のパネラーに、表4に示す評価基準で評価してもらった。その平均点を表3に示す。
(Production Examples 13 to 20)
With the formulation shown in Table 3 below, fried food materials for Production Examples 13 to 20 were produced. The fried dressings of Production Examples 13 to 20 are the same as the fried dressings of Production Examples 17 to 20 and the peptides contained in Production Example 20 in that the molecular weight of the peptides contained in Production Examples 17 to 19 exceeds 800,000. These are comparative examples in that the molecular weight is less than 10,000, and other examples are examples.
(Evaluation test 2)
A batter liquid was produced by mixing 200 parts by mass of water with 100 parts by mass of each fried food material. Tonkatsu was produced by entwining this batter solution with 100 g of pork loin that had been pulverized, adhering bread crumbs, and oiling with salad oil heated to 170 ° C. for 6 minutes.
Freshly fried tonkatsu (after 5 minutes from the oil) and the flavor of tonkatsu left at room temperature (about 25 ° C.) for 1 hour were evaluated by 10 panelists according to the evaluation criteria shown in Table 4. The average points are shown in Table 3.

Figure 2016047570
Figure 2016047570

Figure 2016047570
Figure 2016047570

(製造例21〜30)
下記表5の配合で、製造例21〜30のから揚げ用衣材を製造した。製造例21〜30のから揚げ用衣材は、何れも実施例である。
(評価試験3)
評価試験1と同様にして、製造例21〜28のから揚げ用衣材を用いて鶏肉のから揚げを製造した。揚げたてのから揚げ(油からあげて5分後)、および室温(約25℃)に1時間放置したから揚げの風味を、それぞれ10名のパネラーに、表2に示す評価基準で評価してもらった。その平均点を表5に示す。なお、表5には製造例4の結果を再掲する。
(Production Examples 21 to 30)
The fried dressing materials of Production Examples 21 to 30 were produced according to the formulation shown in Table 5 below. The fried dressing materials of Production Examples 21 to 30 are all examples.
(Evaluation Test 3)
In the same manner as in the evaluation test 1, chicken fried chicken was manufactured using the fried chicken materials of Production Examples 21 to 28. Freshly fried (after 5 minutes from the oil) and left at room temperature (about 25 ° C) for 1 hour, so the flavor of fried food was evaluated by 10 panelists according to the evaluation criteria shown in Table 2 . The average score is shown in Table 5. Table 5 shows the results of Production Example 4 again.

Figure 2016047570
Figure 2016047570

表1及び表3に示す結果から、タウリンと特定の分子量のペプチドの配合した実施例の呈味増強材によれば、食材の風味を活かし、揚げ物独特の風味も維持しながら、コク味及び総合的な風味を向上させることができることが判る。また、表5に示す結果から、酸性アミノ酸、炭化水素アミノ酸、グリシン及びこれらの塩の一種以上を更に配合することにより、更に深い味わいが得られることが判る。   From the results shown in Table 1 and Table 3, according to the taste enhancing material of the examples in which taurine and a peptide having a specific molecular weight are blended, the flavor of the food is utilized and the flavor unique to the deep-fried food is maintained, while the richness and the comprehensiveness are maintained. It can be seen that the typical flavor can be improved. In addition, the results shown in Table 5 indicate that a deeper taste can be obtained by further blending one or more of acidic amino acids, hydrocarbon amino acids, glycine and salts thereof.

本発明の呈味増強剤は、揚げ物用の通常の調味料、打ち粉又は衣材等に添加したり、揚げ物用の通常の調味料、打ち粉又は衣材等として用いたりして、通常の方法で揚げ物を製造するだけで、コク味を有し、油のしつこさ等も感じさせにくい揚げ物を得ることができる。本発明の揚げ物の製造方法によれば、容易に、コク味を有し、油のしつこさ等も感じさせにくい揚げ物を得ることができる。コク味とは、味香りに厚みがあり、口中に広がった風味が長く続き、深い味わいを有することをいう。
The taste enhancer of the present invention is added to a normal seasoning for fried foods, flour or clothes, or used as a normal seasoning for fried foods, flour or clothes, etc. By only producing the fried food by the method, it is possible to obtain a fried food that has a rich taste and is difficult to feel oily persistentness. According to the method for producing fried food of the present invention, it is possible to easily obtain a fried food that has a rich taste and is less likely to feel oily persistence. The rich taste means that the flavor fragrance is thick, the flavor spread in the mouth lasts long, and has a deep taste.

Claims (7)

タウリン及び、動物性又は植物性蛋白の抽出物由来の分子量80万〜1万のペプチドの混合物を含有する揚げ物の呈味増強剤。   A fried food taste enhancer comprising a mixture of taurine and a peptide having a molecular weight of 800,000 to 10,000 derived from an extract of animal or vegetable protein. 前記ペプチドが、分子量50万〜5万のペプチドである、請求項1に記載の呈味増強剤。   The taste enhancer according to claim 1, wherein the peptide is a peptide having a molecular weight of 500,000 to 50,000. 更に酸性アミノ酸、炭化水素アミノ酸、グリシン及びこれらの塩から選択される1種以上を配合する、請求項1又は2に記載の呈味増強剤。   Furthermore, the taste enhancer of Claim 1 or 2 which mix | blends 1 or more types selected from an acidic amino acid, a hydrocarbon amino acid, glycine, and these salts. 揚げ物用の調味料、打ち粉又は衣材である、請求項1〜3のいずれか1項に記載の呈味増強剤。   The taste enhancer according to any one of claims 1 to 3, which is a seasoning for fried food, a dusting powder, or a clothing material. 前記タウリンの配合量が0.01〜5質量%である、請求項4に記載の呈味増強剤。   The taste enhancer of Claim 4 whose compounding quantity of the said taurine is 0.01-5 mass%. 前記ペプチドの配合量が0.01〜5質量%である、請求項4又は5の呈味増強剤。   The taste enhancer of Claim 4 or 5 whose compounding quantity of the said peptide is 0.01-5 mass%. 具材100質量部に対して、タウリン0.001〜0.5質量部、及び動物性又は植物性蛋白の抽出物由来の分子量が80万〜1万のペプチドの混合物0.001〜0.5質量部を付着させた後、油ちょうする揚げ物の製造方法。
0.001 to 0.5 parts by mass of taurine and 100 to 100 parts by mass of ingredients 0.001 to 0.5 of a peptide having a molecular weight derived from animal or vegetable protein extracts of 800,000 to 10,000 The manufacturing method of the deep-fried food which oils after attaching a mass part.
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