JPWO2007119401A1 - Blood component collection device - Google Patents

Abstract

When the blood component collection device shifts from the first plasma collection step to the first constant-speed plasma circulation step, and when the blood component collection device shifts from the second plasma collection step to the second constant-speed plasma circulation step, The liquid feed amount of the second liquid feed pump is increased while continuing the operation of the first liquid feed pump. Thus, in the first constant-speed plasma circulation step and the second constant-speed plasma circulation step, the plasma collected in the plasma collection bag is collected through the plasma circulation line or the like while collecting the plasma in the plasma collection bag. The blood is circulated in the blood storage space of the centrifugal separator, and the flow rate of the liquid component introduced into the blood storage space is made larger than the flow rate of the liquid component introduced into the blood storage space in the plasma collection step.

Description

  The present invention relates to a blood component collection device.

  When collecting blood, the collected blood is separated into each blood component by centrifugation, etc. for reasons such as effective use of the blood and reduction of the burden on the donor, and only the components necessary for the transfuser are collected. Blood is collected to return to the person.

  In such component blood collection, blood collected from a blood donor is introduced into a blood component collection circuit using a blood component collection device, and plasma is collected by a centrifuge called a centrifuge bowl installed in the blood component collection circuit. The buffy coat and red blood cells are separated, the platelets (platelet containing plasma) are separated from the buffy coat, the platelets containing plasma are collected in a platelet collection bag to obtain a platelet preparation, and the plasma is also collected in the plasma collection bag. The remaining plasma, white blood cells, and red blood cells are used as raw materials for the plasma preparation or the plasma fraction preparation, and are returned to the blood donor (see, for example, Japanese Patent No. 2776988).

  In this blood component collection device, a plasma collection step (draw) for collecting plasma in a plasma collection bag, and the collection of the plasma is stopped (interrupted) so that the plasma collected in the plasma collection bag passes through a centrifuge. Circulating plasma circulation step (dwell), circulating the plasma collected in the plasma collection bag to the centrifuge so that its flow rate increases, and collecting the platelet collection step (surge) to collect platelets in the platelet collection bag, and the rest And a blood return step (return) for returning the blood components to the donor. In the plasma collection step, the plasma collected in the plasma collection bag is also circulated so as to pass through the centrifuge.

  However, in the conventional blood component collection device, since the collection of plasma is stopped and the plasma circulation process is performed, it takes a long time to collect blood, which increases the occupation time of the blood component collection device. There is a disadvantage of increasing the burden.

An object of the present invention is to provide a blood component collection device capable of shortening the blood collection time while increasing the recovery rate of collected blood cell components.
In order to achieve the above object, the blood component collection device of the present invention comprises:
A blood collection means comprising a hollow needle for collecting blood from a donor,
A blood separator for separating blood collected by the blood collecting means;
A plasma collection bag for collecting plasma separated by the blood separator;
A blood component collection bag for collecting predetermined blood cell components separated by the blood separator;
A blood treatment line connecting the hollow needle and the inlet of the blood separator;
A blood component collection circuit comprising a plasma circulation line branched from a branch portion provided in the blood treatment line and connected to the plasma collection bag;
A first liquid feeding means installed in the blood processing line and for feeding at least a fluid in the blood processing line;
A second liquid feeding means that is installed in the plasma circulation line and feeds at least the plasma collected in the plasma collection bag;
The collected blood is separated and collected in the plasma collection bag by the plasma collection step of collecting plasma in the plasma collection bag by the operation of the first liquid feeding means and the operation of the second liquid delivery means. Blood component that collects blood by executing a plasma circulation step of circulating the collected plasma to the blood separator via a plasma circulation line and a blood component collection step of collecting a predetermined blood cell component in the blood component collection bag A collecting device,
When shifting from the plasma collection step to the plasma circulation step, the liquid supply amount of the second liquid supply unit is increased while continuing the operation of the first liquid supply unit, whereby the plasma circulation step In this case, while collecting the plasma in the plasma collection bag, the plasma collected in the plasma collection bag is circulated to the blood separator via the plasma circulation line, and the liquid component introduced into the blood separator Is configured to be larger than the flow rate of the liquid component introduced into the blood separator in the plasma collection step.
According to the present invention as described above, the plasma circulation step is executed in which the flow rate of the liquid component introduced into the blood separator is set to be relatively large, that is, larger than the flow rate in the plasma collection step. Therefore, for example, blood cell components (eg, platelets) to be collected that are confined by the separated red blood cell layer or the like can be reliably washed out. Thereby, the recovery rate of the blood cell component can be improved.
In the plasma circulation process, blood is collected from a donor and collected in a plasma collection bag, so that the blood collection time can be shortened. Thereby, the occupation time of the blood component collection device can be reduced, and the burden on the blood donor can be reduced.

In the blood component collection device of the present invention, the flow rate of the liquid component introduced into the blood separator in the plasma circulation step is preferably 40 to 250 mL / min.
As a result, it is possible to surely wash out the blood cell component to be collected confined by the separated red blood cell layer while preventing the blood cell component to flow out of the blood separator, and to reduce the internal viscosity of the buffy coat layer. An excessive rise can be prevented, and the recovery rate of the collected blood cell components can be improved.

In the blood component collection device of the present invention, the difference between the flow rate of the liquid component introduced into the blood separator in the plasma circulation step and the flow rate of the liquid component introduced into the blood separator in the plasma collection step. Is preferably 10 to 220 mL / min.
As a result, it is possible to surely wash out the blood cell component to be collected confined by the separated red blood cell layer while preventing the blood cell component to flow out of the blood separator, and to reduce the internal viscosity of the buffy coat layer. An excessive rise can be prevented, and the recovery rate of the collected blood cell components can be improved.

In the blood component collection device of the present invention, in the plasma collection step, the second liquid feeding means is activated, and the plasma collected in the plasma collection bag is circulated to the blood separator via the plasma circulation line. It is preferable to be configured so that the
Thereby, the collection rate of the collected blood cell components can be further improved.

In the blood component collection device of the present invention, in the plasma collection step, the operation of the second liquid feeding means is controlled so that the flow rate of the liquid component introduced into the blood separator becomes a predetermined target value. It is preferable that the second liquid feeding means is configured to adjust the liquid feeding amount.
As a result, an excessive load is applied to the blood and blood components in the blood separator due to a decrease in the amount of blood collected (for example, excessive centrifugation when a centrifuge is used as the blood separator), and the blood cell components to be collected It can prevent that a recovery rate falls.

In the blood component collection device of the present invention, in the plasma circulation step, the operation of the second liquid feeding means is controlled so that the flow rate of the liquid component introduced into the blood separator becomes a predetermined target value. It is preferable that the second liquid feeding means is configured to adjust the liquid feeding amount.
As a result, an excessive load is applied to the blood and blood components in the blood separator due to a decrease in the amount of blood collected (for example, excessive centrifugation when a centrifuge is used as the blood separator), and the blood cell components to be collected It can prevent that a recovery rate falls.

The blood component collection device of the present invention is configured to execute the plasma collection step a plurality of times before the blood component collection step, and to execute the plasma circulation step between each plasma collection step. It is preferable.
Thereby, the collection rate of the collected blood cell components can be further improved.

In the blood component collection device of the present invention, the blood component collection device comprises the plasma collection step, the plasma circulation step, the blood component collection step, and a blood return step for returning the remaining blood components to the donor. It is preferable that at least one cycle of the blood component collecting operation is performed.
Thereby, since a blood collection process (plasma collection process) and a blood return process can be repeatedly performed using one blood collection needle (hollow needle), a donor (donor) may be restrained in both arms. In addition, the burden on the blood donor can be reduced.

In the blood component collecting apparatus of the present invention, the predetermined blood cell component is preferably platelets.
Thereby, platelets confined by the separated red blood cell layer or the like can be reliably washed out, and the platelet recovery rate can be improved.

The blood component collection device of the present invention comprises:
A blood collection means comprising a hollow needle for collecting blood from a donor,
A centrifuge for separating blood collected by the blood collection means;
A plasma collection bag for collecting plasma components separated by the centrifuge;
A platelet collection bag for collecting the platelet components separated by the centrifuge;
A blood treatment line connecting the hollow needle and the inlet of the centrifuge;
A blood component collection circuit comprising a plasma circulation line branched from a branch portion provided in the blood treatment line and connected to the plasma collection bag;
A first liquid feeding means installed in the blood processing line for transferring a liquid in the blood processing line;
A second liquid feeding means installed in the plasma circulation line for transferring the liquid in the plasma collection bag;
A blood collecting step of operating the first liquid feeding means, transferring the blood collected by the blood collecting means to the centrifuge, and collecting the plasma component separated by the centrifuge in the plasma collection bag; Plasma that circulates the plasma component between the plasma collection bag and the centrifuge by operating the second liquid feeding means, transferring the plasma component collected in the plasma collection bag to the centrifuge A circulation step and the second liquid feeding means is operated to transfer the plasma component collected in the plasma collection bag while accelerating at a predetermined acceleration, and the platelet component flowing out from the centrifuge is collected in the platelet collection A blood component collection device having a first liquid feeding means and a control means for controlling the operation of the second liquid feeding means so as to perform a platelet collecting step to be collected in a bag,
The control means operates the second liquid feeding means to operate the plasma circulation without interrupting the blood collecting process while the blood collecting process is being executed by operating the first liquid feeding means. The platelet collection step is executed after the step is executed and the blood collection step is completed.

  In the blood component collection device of the present invention, the control means performs a plurality of plasma circulations without interrupting the blood collection step during the blood collection step before performing the platelet collection step. Preferably, the process is configured to execute.

  In the blood component collection device of the present invention, it is preferable that the control means is configured to execute the platelet collection step after performing the blood collection step alone.

FIG. 1 is a plan view showing a first embodiment of a blood component collection device of the present invention. FIG. 2 is a partially cutaway sectional view showing a state in which the centrifuge is mounted on the centrifuge driving device provided in the blood component collection device shown in FIG. FIG. 3 is a flowchart for explaining the operation of the blood component collection device shown in FIG. FIG. 4 is a flowchart for explaining the operation of the blood component collection device shown in FIG. FIG. 5 is a diagram for explaining the characteristics of the blood component collection device shown in FIG. 1. FIG. 6 is a view for explaining the operation control of the second liquid feeding pump of the blood component collection device shown in FIG. FIG. 7 is a view for explaining another operation control of the second liquid feeding pump of the blood component collection device shown in FIG.

  Hereinafter, the blood component collection device of the present invention will be described in detail based on preferred embodiments shown in the accompanying drawings.

  FIG. 1 is a plan view showing a first embodiment of a blood component collection device of the present invention. FIG. 2 is a diagram showing a centrifugal separator mounted on the centrifuge drive device provided in the blood component collection device shown in FIG. It is a partially broken sectional view of a state.

  A blood component collection apparatus 1 shown in FIG. 1 separates blood into a plurality of blood components, and separates blood platelets (platelets including plasma) (blood components), which are predetermined blood cell components, and plasma (blood components). It is a device for collecting. The blood component collection device 1 has a rotor 142 having a blood storage space 146 therein, an inlet 143 communicating with the blood storage space 146, and an outlet (outlet) 144. The rotor 142 rotates to rotate the inlet 142 from the inlet 143. A centrifuge (blood separator) 20 that centrifuges the introduced blood in the blood storage space 146, a first line 21 that connects a blood collection needle (blood collection means) 29 and the inlet 143 of the centrifuge 20; The second line 22 connected to the outlet 144 of the centrifuge 20, the third line 23 connected to the first line 21, and the first line 21 via the tubes 49 and 50. And a plasma collection bag (collection bag) 25 connected to the second line 22 via the tubes 43 and 44, and an air connected to the second line 22 via the tube 42. The bag 27b, an intermediate bag (temporary storage bag) (collection bag) 27a connected to the second line 22 via the tubes 43 and 45, and an intermediate bag 27a connected via the tubes 46, 47 and 48 A blood component collection circuit (collection circuit) 2 having a platelet collection bag (collection bag) 26 and a bag 28 connected to the platelet collection bag 26 via a tube 51 is provided.

  Further, the blood component collecting device 1 includes a centrifuge driving device 10 for rotating the rotor 142 of the centrifuge 20, and a first liquid feeding pump (first liquid feeding means) for the first line 21. ) 11, a second liquid feed pump (second liquid feed means) 12, a third liquid feed pump (third liquid feed means) 13 for the third line 23, and a blood component collection circuit A plurality of (sixth, first to sixth in this embodiment) channel opening / closing means 81, 82, 83, 84, 85, 86 that can open and close the middle of the two channels, and the centrifuge drive device 10. A control unit (control means) 3 for controlling the first liquid delivery pump 11, the second liquid delivery pump 12, the third liquid delivery pump 13, and the plurality of flow path opening / closing means 81 to 86, and turbidity Sensor (platelet concentration sensor) 14, optical sensor 15, weight sensor 16, and a plurality (this embodiment) It is provided with a bubble sensor 31,32,33,34,35,36 of six).

First, the blood component collection circuit 2 will be described.
This blood component collection circuit 2 connects a blood collection needle (hollow needle) (blood collection means) 29 for collecting blood from a donor (blood donor) and an inlet 143 of the centrifuge 20 and connects the first pump tube 21g. A first line 21 (blood collection and blood return line) (blood treatment line) 21 that is also used as both a blood collection line and a blood return line, and one end side is connected to an outlet (outlet) 144 of the centrifuge 20 The second line 22 formed, the third line (anticoagulant injection line) 23 connected to the blood collection needle 29 of the first line 21 and including the third pump tube 23a, and the first line 21 is connected to the centrifuge 20 side from the first pump tube 21g, a tube 49 connected to the tube 50, a part of which constitutes the second pump tube 22a, and a second line. 22, a tube 44 connected to the tube 43, a plasma collection bag 25 connected to the tubes 44 and 49, a tube 42 connected to the second line 22, and a connection to the tube 42 Air bag 27b, tube 45 connected to tube 43, intermediate bag 27a connected to tube 45, tube 46 connected to intermediate bag 27a, tube 47 connected to tube 46, tube 48, a platelet collection bag 26 connected to the tube 48, a tube 51 connected to the platelet collection bag 26, and a bag 28 connected to the tube 51. The air bag 27b and the intermediate bag 27a are integrally formed (integrated).

  The first line 21 is connected to the blood collection needle side first line 21a to which the blood collection needle 29 is connected, one end side is connected to the blood collection needle side first line 21a, and the other end side is connected to the inlet 143 of the centrifuge 20. Centrifuge side first line 21b. As the blood collection needle 29, for example, a known metal needle is used.

  The blood collection needle side first line 21a, the centrifuge side first line 21b, the second line 22 and the third line 23 described later are each connected by a soft resin tube or a plurality of soft resin tubes. Has been formed.

  The blood collection needle side first line 21a is connected to the third line 23 from the blood collection needle 29 side, the chamber 21d for removing bubbles and microaggregates, and the branch connector for connection to the tube 50. And a first pump tube 21g formed between the chamber 21d and the branch connector 21f.

  Air bubble sensors 35, 36 and 32 are installed along the blood collection needle side first line 21a from the blood collection needle 29 side. In this case, the bubble sensors 35 and 36 are disposed between the branch connector 21c and the chamber 21d, and the bubble sensor 32 is disposed between the chamber 21d and the first pump tube 21g.

  The bubble sensors 35, 36, and 32 transmit and receive ultrasonic waves from the outside of the tube, and make use of the fact that the ultrasonic conductivity differs between the liquid and the bubbles (gas), so that the gas and liquid (gas / liquid) in the tube are used. Or a gas / liquid level). The bubble sensors 31, 33 and 34 are also detection means having the same function as described above. Further, the bubble sensor (gas and liquid detection means) is not limited to the ultrasonic sensor, and for example, an optical sensor, an infrared sensor, or the like may be used.

  The chamber 21d is connected with a gas-permeable and bacteria-impermeable filter 21i through a tube 21h. This line can be used for detecting the internal pressure of the blood collection needle side first line 21a, for example.

  On the other hand, the centrifuge side first line 21 b is connected to a branch connector 21 f for connection with the tube 50.

One end of the second line 22 is connected to the outlet 144 of the centrifuge 20.
The second line 22 includes a branch connector 22b for connection to the tubes 42 and 43.

  A turbidity sensor 14 and a bubble sensor 34 are installed along the second line 22 from the centrifuge 20 side. In this case, the turbidity sensor 14 and the bubble sensor 34 are disposed between the centrifuge 20 and the branch connector 22b.

  The branch connector 22b is connected with a filter 22f that is air-permeable and bacteria-impermeable through a tube 41. This line can be used for detecting the internal pressure of the second line 22, for example.

  One end of the third line 23 is connected to a connecting branch connector 21 c provided on the first line 21. That is, the third line (flow path) 23 branches from the first line (flow path) 21 via the branch connector (branch portion) 21c. The branch connector 21c is located (provided) in the vicinity of the blood collection needle 29.

  The third line 23 includes, from the branch connector 21c side, a third pump tube 23a, a sterilizing filter (foreign matter removing filter) 23b, a bubble removing chamber 23c, and an anticoagulant container connecting needle 23d. It has.

  A bubble sensor 31 is installed along the third line 23. The bubble sensor 31 is disposed between the branch connector 21c and the third pump tube 23a.

  The anticoagulant container connecting needle 23d of the third line 23 is connected to a container (not shown) in which an anticoagulant (anticoagulant liquid) is housed (contained), whereby the anticoagulant in the container is As will be described later, the anticoagulant container connecting needle 23d flows through the third line 23 toward the branch connector 21c and is supplied (injected) to the blood collection needle side first line 21a. Thereby, for example, the anticoagulant can be added (mixed) to the blood collected by the blood collection needle 29 via the third line 23.

  In addition, although it does not specifically limit as an anticoagulant, For example, ACD-A liquid etc. can be used.

  The plasma collection bag 25, which is a blood component collection bag, is a container for collecting (storing) plasma (plasma component) (second blood component). One end of the tube 49 is connected to the plasma collection bag 25, and a connecting branch connector 22d is provided in the middle thereof. One end of the tube 50 is connected to the branch connector 22d, and the other end is connected to the branch connector 21f. The second pump tube 22a is located between the plasma collection bag 25 and the branch connector 22d. The tubes 49 and 50 and the branch connector 22d constitute the main part of the plasma circulation line 24.

  One end of the tube 43 is connected to the branch connector 22b, and the other end is provided with a connection branch connector 22c. One end of the tube 44 is connected to the branch connector 22c, and the other end is connected to the plasma collection bag 25.

  A bubble sensor 33 is installed along the tube 46 in the middle of the tube 46.

  The plasma collection bag 25 and tubes 43 and 44 constitute a plasma collection branch line for collecting plasma.

  A platelet (platelet preparation) collection bag 26, which is a blood component collection bag, collects (stores) platelets (platelet component) (blood cell component) (first blood component) containing plasma after passing through a leukocyte removal filter 261 described later. ). In the following description, platelets including plasma (first blood component) are referred to as “concentrated platelets”, and concentrated platelets collected (stored) in the platelet collection bag 26 are referred to as “platelet preparations”.

  One end of the tube 51 is connected to the platelet collection bag 26, and the bag 28 is connected to the other end.

  The air bag 27b is a container for temporarily storing (reserving) air.

  At the time of blood collection to be described later, air (sterilized air) in the blood component collection circuit 2 such as in the blood storage space 146 of the centrifuge 20 is transferred and stored in the air bag 27b. In the blood return process (blood component return process), the air stored in the air bag 27b is transferred into the blood storage space 146 of the centrifuge 20 and returned. Thereby, a predetermined blood component is returned to the donor.

  One end of the tube 42 is connected to the branch connector 22b, and the other end is connected to the airbag 27b.

  The intermediate bag (temporary storage bag) 27a which is a blood component collection bag is a container (storage unit) for temporarily storing concentrated platelets, that is, platelets containing blood (blood cell components) (first blood component). is there. One end of the tube 45 is connected to the branch connector 22c, and the other end is connected to the intermediate bag 27a.

  One end of the tube 46 is connected to the intermediate bag 27a, and a connecting branch connector 22e is provided at the other end. The other end of the tube 49 is connected to the branch connector 22e.

  In addition, one end of a tube 47 is connected to the branch connector 22e for connection, and a leukocyte removal filter (cell separation filter) (filtering) that separates and removes leukocytes (predetermined cells) from the concentrated platelets is provided in the middle of the tube 47. 261) is installed.

  The other end of the tube 47 is provided with a connecting branch connector 22g, and the other end of the tube 48, one end of which is connected to the platelet collection bag 26, is connected to the branch connector 22g.

  Further, a filter main body provided with a vent filter and a filter 22h provided with a cap are installed at the port of the branch connector 22g.

  Here, in a filtration operation for separating and removing leukocytes in the concentrated platelets described later, the tubes 46 and 47 constitute a supply tube for supplying the concentrated platelets from the intermediate bag 27a to the leukocyte removal filter 261. Constitutes a discharge tube for discharging the concentrated platelets after the white blood cells have been separated and removed from the white blood cell removal filter 261 (supplied to the platelet collection bag 26).

  That is, the tubes 46, 47, 48, the intermediate bag 27a, the leukocyte removal filter 261 and the platelet collection bag 26 constitute a filtration line for separating and removing leukocytes from the concentrated platelets.

  With the blood component collection device 1 assembled (when the blood component collection device 1 is used), the intermediate bag 27a, the leukocyte removal filter 261, the platelet collection bag 26, and the plasma collection bag 25 are respectively the intermediate bag 27a. The leukocyte removal filter 261 is set at a position lower than the plasma collection bag 25 (downward in the vertical direction) at a position lower than the intermediate bag 27a, and the platelet collection bag 26 is set at a position lower than the leukocyte removal filter 261 (positioned). The intermediate bag 27a and the plasma collection bag 25 are respectively positioned higher (vertically upward) than the blood storage space 146 of the rotor 142 of the centrifuge 20.

  In this case, the blood component collection device 1 is provided with hangers (hooks) (not shown), which are support portions that detachably support the plasma collection bag 25, the intermediate bag 27a, and the air bag 27b. The plasma collection bag 25 and the intermediate bag 27a are respectively hooked and hung (suspended) on the corresponding hangers so that the outlet side (inlet side) is vertically downward.

  Further, as the leukocyte removal filter 261, for example, in a casing having an inlet and an outlet at both ends, for example, a woven fabric, a nonwoven fabric, a mesh, a foam or the like made of a synthetic resin such as polypropylene, polyester, polyurethane, polyamide, etc. The thing constituted by inserting the filtration member which laminated one layer or two layers or more of a porous body can be used.

  As the constituent materials of the tubes used for forming the first to third lines 21 to 23 and the pump tubes 21g, 22a and 23a, and the other tubes 41 to 51 and 21h, respectively, Vinyl chloride is preferred.

  If these tubes are made of polyvinyl chloride, sufficient flexibility and softness can be obtained, so that they are easy to handle and are suitable for clogging with a clamp or the like.

  Further, as the constituent materials of the branch connectors 21c, 21f, 22b, 22c, 22d, 22e, and 22g described above, the same materials as those described for the tube can be used.

  In addition, as each pump tube 21g, 22a, 23a, what has the intensity | strength of the grade which is not damaged even if it presses by each liquid feeding pump (for example, roller pump etc.) 11, 12, 13 mentioned later, respectively is used. Has been.

  Each of the plasma collection bag 25, the platelet collection bag 26, the intermediate bag 27a, the air bag 27b, and the bag 28 is laminated with a resin-made flexible sheet material, and the peripheral portions thereof are fused (thermal fusion, high frequency fusion). Or a bag formed by bonding with an adhesive or the like. As described above, the air bag 27b and the intermediate bag 27a are integrally formed (integrated).

  As a material used for each bag 25, 26, 27a, 27b, 28, for example, soft polyvinyl chloride is preferably used.

  In addition, as a sheet material used for the platelet collection bag 26, it is more preferable to use a material excellent in gas permeability in order to improve platelet storage stability.

  As such a sheet material, for example, polyolefin, DnDP plasticized polyvinyl chloride, or the like is used, and a sheet material of the above-described material is used without using such a material. What was thin (for example, about 0.1-0.5 mm, especially about 0.1-0.3 mm) is suitable.

  Although the main part of such a blood component collection circuit 2 is not shown, it is of a cassette type, for example. That is, the blood component collection circuit 2 partially stores each line (the first line 21, the second line 22, and the third line 23) and each predetermined tube, and partially holds them. In other words, it comprises a cassette housing in which they are partially fixed.

  In the cassette housing, both ends of the first pump tube 21g, both ends of the second pump tube 22a, and both ends of the third pump tube 23a are fixed. These pump tubes 21g, 22a, and 23a are respectively connected to the cassette housing. From the housing, it protrudes in the shape of a loop corresponding to the shape of each liquid feed pump (for example, roller pump etc.) 11, 12, and 13. Therefore, the first, second, and third pump tubes 21g, 22a, and 23a can be easily attached to the liquid feeding pumps 11, 12, and 13, respectively. The cassette housing is provided with respective flow path opening / closing means 81 to 86 described later.

  The centrifuge 20 provided in the blood component collection circuit 2 is generally called a centrifuge bowl, and separates blood into a plurality of blood components by centrifugal force.

  As shown in FIG. 2, the centrifuge 20 has a vertically extending tube 141 with an inlet 143 formed at the upper end, and rotates around the tube 141 and is liquid-tightly sealed with respect to the upper portion 145. And a hollow rotor 142.

  An annular blood storage space 146 is formed in the rotor 142 along the inner surface of the peripheral wall. The blood storage space 146 has a shape (tapered shape) in which the inner and outer diameters gradually decrease from the lower part toward the upper part in FIG. 2, and the lower part is formed in a substantially disc shape formed along the bottom part of the rotor 142. The upper end of the tubular body 141 communicates with the discharge port (outlet) 144. Further, in the rotor 142, the volume of the blood storage space 146 is, for example, about 100 to 350 mL, and the maximum inner diameter (maximum radius) from the rotating shaft of the rotor 142 is, for example, about 55 to 65 mm.

  Such a rotor 142 rotates under predetermined centrifugal conditions (rotation speed and rotation time) set in advance by the centrifuge drive device 10 included in the blood component collection device 1. Under this centrifugal condition, a blood separation pattern (for example, the number of blood components to be separated) in the rotor 142 can be set.

  In the present embodiment, as shown in FIG. 2, the centrifugal conditions are set so that the blood is separated from the inner layer into the plasma layer 131, the buffy coat layer 132, and the red blood cell layer 133 in the blood storage space 146 of the rotor 142.

Next, the overall configuration of the blood component collection device 1 shown in FIG. 1 will be described.
The blood component collection device 1 includes a centrifuge driving device 10 for rotating the rotor 142 of the centrifuge 20, a first liquid feeding pump 11 installed in the middle of the first line 21, and a tube 49. The second liquid delivery pump 12 installed in the middle, the third liquid delivery pump 13 installed in the middle of the third line 23, and the blood component collection circuit 2 (first line 21, tube 42, tube 44, tube 45, tube 47, tube 49), a plurality of flow path opening / closing means 81, 82, 83, 84, 85, 86 that can open and close the flow path, and display means for displaying (notifying) various information. (Notification means) and a display / operation section 17 which is an operation means for performing each operation, a storage section (storage means) 18, a centrifuge drive device 10, a first liquid feed pump 11, a second liquid feed pump 12. Third liquid pump 3, and a control section (control means) 3 for controlling the plurality of flow path shutter means 81 to 86, units such as the display and operation unit 17 and the storage unit 18.

  Furthermore, the blood component collection device 1 includes a turbidity sensor 14 mounted (installed) on the second line 22, an optical sensor 15 installed near the centrifuge 20, and a plurality of bubble sensors 31 to 36. And a weight sensor 16 for measuring the weight of the plasma together with the plasma collection bag 25.

  The control unit 3 includes three pump controllers (not shown) for the first liquid feeding pump 11, the second liquid feeding pump 12, and the third liquid feeding pump 13, and includes the control unit 3 and the first liquid feeding pump 13. The liquid feed pump 11, the second liquid feed pump 12, and the third liquid feed pump 13 are electrically connected via a pump controller.

  A drive controller (not shown) included in the centrifuge drive device 10 is electrically connected to the control unit 3.

Each of the channel opening / closing means 81 to 86 is electrically connected to the control unit 3.
The turbidity sensor 14, the optical sensor 15, the weight sensor 16, the bubble sensors 31 to 36, the display / operation unit 17, and the storage unit 18 are each electrically connected to the control unit 3.

  The control unit 3 is configured by, for example, a microcomputer (including a calculation unit, a memory, and the like). The control unit 3 includes the turbidity sensor 14, the optical sensor 15, the weight sensor 16, and the bubble sensors 31 to 36 described above. The detection signals from are input as needed. A signal (input) from the display / operation unit 17 is also input to the control unit 3.

  The control unit 3 collects blood components according to a preset program based on the detection signals from the turbidity sensor 14, the optical sensor 15, the weight sensor 16, the bubble sensors 31 to 36 and the signal from the display / operation unit 17. The operation of each part of the apparatus 1, that is, the rotation, stop, and rotation direction (forward / reverse rotation) of each liquid feed pump 11, 12, 13 is controlled, and the flow path opening / closing means 81-86 are opened and closed as necessary. The operation of the centrifuge drive device 10 and the drive of the display / operation unit 17 are controlled.

  The first flow path opening / closing means 81 is provided to open and close the first line 21 from the first pump tube 21g to the blood collection needle 29 side, that is, between the first pump tube 21g and the chamber 21d. Yes.

  The second flow path opening / closing means 82 is provided for opening and closing the tube 47. The third flow path opening / closing means 83 is provided for opening and closing the tube 44. The fourth flow path opening / closing means 84 is provided to open and close the tube 45. The fifth flow path opening / closing means 85 is provided for opening and closing the tube 42. The sixth flow path opening / closing means 86 is provided to open and close the tube 49 between the branch connector 22d and the branch connector 22e.

  Each flow path opening / closing means 81-86 includes an insertion part into which the first line 21 and tubes 47, 44, 45, 42, 49 can be inserted. The insertion part includes, for example, a solenoid, an electric motor, It has a clamp that operates with a drive source such as a cylinder (hydraulic or pneumatic). Specifically, an electromagnetic clamp that operates with a solenoid is suitable.

  These flow path opening / closing means (clamps) 81 to 86 are operated based on signals from the control unit 3.

  The display / operation unit 17 includes, for example, a touch panel including a liquid crystal display panel, an EL display panel, and the like. The display / operation unit 17 constitutes input means for inputting predetermined information and data (for example, an initial value of the target number of platelets (blood cell components) to be collected, a blood count of a donor (donor), etc.).

  In addition, a display unit (for example, a liquid crystal display panel, an EL display panel, etc.) that is a display unit (notification unit) for displaying (notifying) various information, and an operation unit (for example, an operation button) that is an operation unit for performing each operation , An operation switch, an operation dial, etc.) may be provided separately.

  The storage unit 18 includes a storage medium (also referred to as a recording medium) in which various information, data, tables, arithmetic expressions, programs, and the like are stored (also referred to as a recording medium). It is composed of various semiconductor memories, IC memories, and the like, such as volatile memories such as RAM, nonvolatile memories such as ROM, rewritable (erasable and rewritable) nonvolatile memories such as EPROM, EEPROM, and flash memory. Control such as writing (storage), rewriting, erasing, and reading in the storage unit 18 is performed by the control unit 3.

  As shown in FIG. 2, the centrifuge drive device 10 includes a housing 201 that houses the centrifuge 20, a leg portion 202, a motor 203 that is a drive source, and a disk-shaped fixing that holds the centrifuge 20. And a table 205.

  The housing 201 is placed and fixed on the upper portion of the leg portion 202. In addition, a motor 203 is fixed to the lower surface of the housing 201 via a spacer 207 with bolts 206.

  A fixed base 205 is fitted on the tip of the rotating shaft 204 of the motor 203 so as to rotate coaxially and integrally with the rotating shaft 204, and the bottom of the rotor 142 is fitted on the upper portion of the fixed base 205. A concave portion is formed.

  The upper portion 145 of the centrifuge 20 is fixed to the housing 201 by a fixing member (not shown).

  In such a centrifuge drive device 10, when the motor 203 is driven, the fixed base 205 and the rotor 142 fixed thereto rotate at, for example, about 3000 to 6000 rpm.

  The optical sensor 15 is installed on the side of the housing 201 (left side in FIG. 2).

  The optical sensor 15 is configured to project light toward the blood storage space 146 and to receive the reflected light.

  The optical sensor 15 irradiates (projects) light (for example, laser light) from the light projecting unit 151, and the reflected light reflected by the reflecting surface 147 of the rotor 142 is received by the light receiving unit 152. The light receiving unit 152 converts the received light quantity into an electrical signal.

  Here, the optical sensor 15 has a reflecting surface on one side and a reflecting plate 153 that changes the optical path, and the light irradiated from the light projecting unit 151 passes through the reflecting plate 153 and is reflected on the reflecting surface 147. The light that is irradiated to the light and reflected by the reflecting surface 147 is received by the light receiving unit 152 via the reflecting plate 153.

  At this time, the projection light and the reflected light are transmitted through the blood component in the blood storage space 146, but the position of the blood component interface (the interface B between the plasma layer 131 and the buffy coat layer 132 in this embodiment). Accordingly, since the abundance ratio of each blood component at a position where the light projection light and the reflected light are transmitted is different, the transmittance thereof is changed. As a result, the amount of light received by the light receiving unit 152 varies (changes), and this variation can be detected as a change in the output voltage from the light receiving unit 152.

  That is, the optical sensor 15 can detect the position of the blood component interface based on the change in the amount of light received by the light receiving unit 152.

  The interface of the blood component detected by the optical sensor 15 is not limited to the interface B, and may be the interface between the buffy coat layer 132 and the red blood cell layer 133, for example.

  Here, each of the layers 131 to 133 in the blood storage space 146 has a different color depending on the blood component, and in particular, the red blood cell layer 133 is red with the color of the red blood cells. For this reason, from the viewpoint of improving the accuracy of the optical sensor 15, there is a range suitable for the wavelength of the projection light, and the wavelength range is not particularly limited, but is preferably about 600 to 900 nm, for example. 750 to 800 nm is more preferable.

  The turbidity sensor 14 is for detecting the turbidity (platelet concentration) of the fluid flowing in the second line 22 and outputs a voltage value corresponding to the turbidity. Specifically, the turbidity sensor 14 outputs a low voltage value when the turbidity is high and a high voltage value when the turbidity is low.

  The turbidity sensor 14 can detect, for example, the concentration of platelets in plasma flowing through the second line 22, changes in the platelet concentration in plasma, and contamination of red blood cells into the plasma.

  Further, the bubble sensor 34 can detect, for example, the replacement of the fluid flowing in the second line 22 from air to plasma.

  As the turbidity sensor 14 and the bubble sensors 31 to 36, for example, an ultrasonic sensor, an optical sensor, an infrared sensor, or the like can be used.

  The first liquid feed pump 11 to which the first pump tube 21g is attached, the second liquid feed pump 12 to which the second pump tube 22a is attached, and the third pump to which the third pump tube 23a is attached. As the liquid feeding pump 13, for example, a non-blood contact type pump such as a roller pump is preferably used.

  Further, as the first liquid feeding pump (blood pump) 11, a pump capable of feeding blood in any direction is used. Specifically, a roller pump capable of forward rotation and reverse rotation is used.

  By the operation of the first liquid feeding pump 11, for example, liquid (fluid) such as blood and blood components in the first line 21 can be fed (transferred). Further, by the operation of the second liquid feeding pump 12, for example, plasma (liquid) collected in the plasma collection bag 25 can be sent (transferred). Further, by the operation of the third liquid feed pump 13, for example, a liquid (fluid) such as an anticoagulant (anticoagulant liquid) in the third line 23 can be fed (transferred).

  Here, as will be described later, the blood component collection device 1 includes a first plasma collection step for collecting plasma in the plasma collection bag 25 by the operation of the first liquid feed pump 11, and a second liquid feed pump 12. The first constant-speed plasma circulation step (plasma circulation step) for circulating the plasma collected in the plasma collection bag 25 into the blood storage space 146 of the centrifuge 20 by the operation of, and the operation of the first liquid feeding pump 11 Thus, the second plasma collection step of collecting plasma in the plasma collection bag 25 and the second plasma collection step of circulating the plasma collected in the plasma collection bag 25 into the blood storage space 146 by the operation of the second liquid feeding pump 12. A constant-speed plasma circulation step (plasma circulation step), a third plasma collection step of collecting plasma in the plasma collection bag 25 by the operation of the first liquid feeding pump 11, a platelet collection step (blood component collection step), , Blood return process (blood formation Return step) and platelet collection operation with the (blood component collection operation), and is configured to perform the control of the control unit 3 (see FIG. 5).

  In the first constant-speed plasma circulation step and the second constant-speed plasma circulation step, the plasma circulation line 24, the branch connector 21f, and the centrifuge, each of which includes a tube 49, a branch connector 22d, and a tube 50, respectively. The separator-side first line 21b, the blood storage space 146 of the centrifuge 20, the second line 22, the branch connector 22b, the tube 43, the branch connector 22c, the tube 44, and the plasma collection bag 25 A circulation circuit is formed (configured), and the operation of the second liquid feeding pump 12 causes the plasma in the plasma collection bag 25 to circulate through the circulation circuit (through the blood storage space 146). .

  The first plasma collection step, the second plasma collection step, the third plasma collection step, the first plasma circulation step, and the second plasma circulation step are executed before the platelet collection step. In addition, the first constant-speed plasma circulation step is executed between the first plasma collection step and the second plasma collection step, and the second constant-speed plasma circulation step is the second plasma collection step. And the third plasma collection step. Further, this platelet collection operation is performed at least once (cycle).

  FIG. 5 is a diagram for explaining the feature (operation) of the blood component collection device shown in FIG. 1, and FIG. 6 is a diagram for explaining the operation control of the second liquid feeding pump of the blood component collection device shown in FIG. FIGS. 7A and 7B are diagrams for explaining another operation control of the second liquid feeding pump of the blood component collection device shown in FIG.

  5, 6, and 7, the horizontal axis represents time, and the vertical axis represents the flow rate of the liquid (fluid) introduced into the blood storage space 146 of the centrifuge 20.

  5, 6, and 7 indicate the liquid (fluid) introduced into the blood storage space 146 of the centrifuge 20 by the operation of the first liquid feeding pump 11, that is, blood (anticoagulation). The flow rate of the agent-added blood. The hatched portion is the flow rate of the blood cell component, and the non-hatched portion is the flow rate of the liquid component, that is, plasma (anticoagulant-added plasma).

  5, 6, and 7, a broken line (dotted line) indicates a liquid component that is introduced into the blood storage space 146 of the centrifuge 20 by the operation of the second liquid feeding pump 12, that is, plasma ( It is the flow rate of anticoagulant-added plasma. In other words, the flow rate indicated by the broken line indicates that the plasma collected in the plasma collection bag 25 is moved into the blood storage space 146 of the centrifuge 20 via the plasma circulation line 24 or the like by the operation of the second liquid feeding pump 12. This is the plasma flow rate (circulation flow rate) when circulating.

As shown in FIG. 5, the blood component collection device 1 is configured to transfer from the first plasma collection process to the first constant-speed plasma circulation process, and from the second plasma collection process to the second constant-speed plasma circulation. When shifting to the process, the liquid feeding amount (discharge amount) of the second liquid feeding pump 12 is increased while the operation of the first liquid feeding pump 11 is continued. As a result, in the first constant-speed plasma circulation step and the second constant-speed plasma circulation step, the plasma collected in the plasma collection bag 25 is collected in the plasma collection bag 25 while the plasma is collected in the plasma collection bag 25, respectively. And the like, and the flow rate of the liquid component introduced into the blood storage space 146 is larger than the flow rate of the liquid component introduced into the blood storage space 146 in the plasma collection step. To do. The liquid component includes not only plasma after separation but also plasma in blood that has not been separated, anticoagulant (anticoagulant liquid), and the like.
As described above, the second pump 12 is operated without interrupting the blood collection process while the first pump 11 is operated and the blood collection process of collecting the plasma in the plasma collection bag 25 is being executed. Then, a plasma circulation process (in this embodiment, a plurality of plasma circulation processes) is executed. That is, in each of the first plasma collection step, the second plasma collection step, the third plasma collection step, the first plasma circulation step, and the second plasma circulation step, the first liquid feeding pump 11 is operated. The blood is collected from the donor, the blood is introduced into the blood storage space 146 of the centrifuge 20 and separated, and the plasma is collected in the plasma collection bag 25, whereby the collection of the plasma in the plasma collection bag 25 is continued. Therefore, blood collection time can be shortened. Thereby, the occupation time of the blood component collection device 1 can be reduced, and the burden on the donor can be reduced.

  In addition, in each of the transition from the first plasma collection process to the first constant-speed plasma circulation process and the transition from the second plasma collection process to the second constant-speed plasma circulation process, the second feeding is performed. The flow rate of the liquid component introduced into the blood storage space 146 in each constant-speed plasma circulation step (plasma circulation flow rate) is increased by increasing the liquid feed amount of the liquid pump 12, and the liquid introduced into the blood storage space 146 in the plasma collection step Since it is larger than the flow rate of the sex component (circulation flow rate of plasma), platelets trapped by the separated red blood cell layer can be surely washed out, and excessive increase in the internal viscosity of the buffy coat layer (buffy coat layer) Excessive concentration) can be prevented (blocked), thereby improving the recovery rate (yield) of platelets.

  As shown in FIG. 5, in the present embodiment, in the first plasma collecting step, the second plasma collecting step, and the third plasma collecting step, the second liquid feeding pump 12 is operated, respectively, The plasma collected in the collection bag 25 is circulated in the blood storage space 146 of the centrifuge 20 through the plasma circulation line 24 or the like. Thereby, the collection rate of platelets can be further improved.

  In the first plasma collection step of the first cycle, after a predetermined amount (for example, about 10 to 50 mL) of plasma is collected in the plasma collection bag 25, the second liquid feeding pump 12 is operated, Circulation of plasma collected in the plasma collection bag 25 is started, and FIG. 5 shows the case of the first cycle.

  However, when the target amount of plasma collected in the plasma collection bag 25 is less than the predetermined amount, the plasma is not circulated and is performed when the amount is equal to or greater than the predetermined amount.

  In addition, the rotation speed (number of rotations) of the first liquid delivery pump 11 is determined by the first plasma collection step, the first plasma circulation step, the second plasma collection step, the second plasma circulation step, and the third plasma. It is preferably substantially constant throughout the collection process. That is, it is preferable that the flow rate of the blood cell component introduced into the blood storage space 146 of the centrifuge 20 (flowing through the first line 21) by the operation of the first liquid feeding pump 11 is substantially constant.

  The flow rate of the liquid component introduced into the blood storage space 146 of the centrifuge 20 in each plasma collection step is preferably about 30 to 150 mL / min, and preferably about 40 to 135 mL / min. More preferred.

  And the amount of liquid (circulation flow rate of plasma) of the second liquid pump 12 in each plasma collection step is preferably about 0 to 125 mL / min, and preferably about 5 to 105 mL / min. More preferred.

  The flow rate of the liquid component introduced into the blood storage space 146 in the first plasma collection step, the flow rate of the liquid component introduced into the blood storage space 146 in the second plasma collection step, and the third plasma collection step The flow rate of the liquid component introduced into the blood storage space 146 is preferably substantially the same.

  Further, the flow rate of the liquid component introduced into the blood storage space 146 of the centrifuge 20 in each constant-speed plasma circulation step depends on the rotational speed and blood collection speed of the centrifuge 20, but is 40 to 250 mL / min, respectively. Is preferably about 60 to 200 mL / min.

  Accordingly, it is possible to surely wash out the platelets trapped by the separated red blood cell layer while preventing the platelets from flowing out from the discharge port 144 of the centrifuge 20, and the internal viscosity of the buffy coat layer is excessive. Increase can be prevented, and the recovery rate of platelets can be improved.

  Further, the difference between the flow rate of the liquid component introduced into the blood storage space 146 of the centrifuge 20 in each constant-speed plasma circulation step and the flow rate of the liquid component introduced into the blood storage space 146 in each plasma collection step, that is, The amount of increase in the amount of liquid fed by each second liquid feed pump 12 (plasma circulation flow rate) when moving from each plasma collection step to each constant-speed plasma circulation step is about 10 to 220 mL / min. Is preferable, and it is more preferably about 60 to 200 mL / min.

  Accordingly, it is possible to surely wash out the platelets trapped by the separated red blood cell layer while preventing the platelets from flowing out from the discharge port 144 of the centrifuge 20, and the internal viscosity of the buffy coat layer is excessive. Increase can be prevented, and the recovery rate of platelets can be improved.

  In addition, the flow rate of the liquid component introduced into the blood storage space 146 in the second constant-speed plasma circulation step is smaller than the flow rate of the liquid component introduced into the blood storage space 146 in the first constant-speed plasma circulation step ( Small).

  Specifically, the flow rate of the liquid component introduced into the blood storage space 146 in the first constant-speed plasma circulation step is preferably about 100 to 250 mL / min, and preferably about 120 to 200 mL / min. More preferred.

  Further, the flow rate of the liquid component introduced into the blood storage space 146 in the second constant-speed plasma circulation step is preferably about 40 to 200 mL / min, and more preferably about 60 to 175 mL / min.

  In addition, the flow rate of the liquid component introduced into the blood storage space 146 in the first constant-speed plasma circulation step and the liquid component introduced into the blood storage space 146 in the first plasma collection step, which is the plasma collection step immediately before that. That is, the increase in the amount of liquid fed by the second liquid feed pump 12 (plasma circulation flow rate) when shifting from the first plasma collection step to the first constant-speed plasma circulation step is: It is preferably about 60 to 220 mL / min, and more preferably about 80 to 180 mL / min.

  In addition, the flow rate of the liquid component introduced into the blood storage space 146 in the second constant-speed plasma circulation step, and the liquid component introduced into the blood storage space 146 in the second plasma collection step, which is the plasma collection step immediately before that. That is, the increase in the amount of liquid fed by the second liquid feed pump 12 (plasma circulation flow rate) when shifting from the second plasma collection step to the second constant-speed plasma circulation step is: It is preferably about 15 to 150 mL / min, and more preferably about 70 to 100 mL / min.

  Further, as shown in FIG. 6, the blood component collection device 1 includes a first plasma collection step, a second plasma collection step, a third plasma collection step, a first plasma circulation step, and a second plasma circulation. In each of the steps, the operation of the second liquid feed pump 12 is controlled so that the flow rate of the liquid component introduced into the blood storage space 146 of the centrifuge 20 becomes a predetermined target value (a constant value). The liquid feeding amount of the second liquid feeding pump 12 is adjusted.

  That is, when the flow rate (blood collection speed) of blood collected from the donor decreases, the rotational speed of the first liquid delivery pump 11 is decreased accordingly. For example, as shown in FIG. In the period T, when the amount of blood collected decreases and the flow rate of blood (blood cell component and liquid component) introduced into the blood storage space 146 of the centrifugal separator 20 decreases, the rotation speed of the second liquid feeding pump 12 increases. The flow rate of the liquid component introduced into the blood storage space 146 is increased by increasing the flow rate, that is, the circulation flow rate of plasma (liquid component) collected in the plasma collection bag 25, and a2 = a1.

  As a result, it is possible to prevent the blood collection volume 146 from excessively centrifuging due to a decrease in the amount of collected blood and a reduction in the platelet recovery rate.

  The target value (constant value) can be set or changed by an operation of the display / operation unit 17 by an operator. For example, according to a blood count such as a donor hematocrit value, The target value can be changed as appropriate.

  In each plasma collection step, it is not necessary to circulate plasma by the operation of the second liquid feeding pump 12. In other words, when shifting from each plasma collection step to each constant-speed plasma circulation step, the amount of liquid fed by the second liquid feed pump 12 may be configured to increase from zero.

  Further, in each plasma collection step, the flow rate of the liquid component introduced into the blood storage space 146 of the centrifuge 20 is predetermined even when plasma circulation is not performed by the operation of the second liquid feeding pump 12. It may be configured to perform adjustment (control) to achieve a target value (a constant value). That is, as shown in FIG. 7, the blood component collection device 1 includes a first plasma collection step, a second plasma collection step, a third plasma collection step, a first plasma circulation step, and a second plasma circulation step. , The operation of the second liquid feed pump 12 is controlled so that the flow rate of the liquid component introduced into the blood storage space 146 of the centrifuge 20 becomes a predetermined target value (a constant value). The liquid feed amount of the liquid feed pump 12 may be adjusted.

  For example, as shown in FIG. 7, in the period T, the blood flow (blood collection speed) collected from the donor decreases, and the blood (blood cell component and liquid component) introduced into the blood storage space 146 of the centrifuge 20. When the flow rate of the liquid decreases, the second liquid feeding pump 12 is operated to circulate the plasma (liquid component) collected in the plasma collection bag 25 in the blood storage space 146, thereby introducing the liquid introduced into the blood storage space 146. The flow rate of the sex component is increased so that b2 = b1.

  In addition, the frequency | count of the plasma collection process in each cycle is not limited to 3 times, For example, 1 time may be sufficient, 2 times may be sufficient and 4 times or more may be sufficient.

  In addition, the number of constant-speed plasma circulation steps in each cycle is not limited to two, and may be, for example, once or three or more times.

  The plasma circulation step is not limited to constant-speed plasma circulation in which plasma is circulated at a constant flow rate (speed). For example, accelerated plasma circulation in which plasma is circulated while increasing the flow rate (velocity) of plasma may be performed.

By the way, the number of “1 unit” of platelets is 0.2 × 10 ¹¹ , and the following four types (1) to (4) are used as platelet preparations (those defined in the preparation standard). There is.

(1) 5 unit preparation The volume (volume) is 100 mL ± 20% and the number is 1.0 × 10 ^{11 to} 1.9 × 10 ¹¹ (2) 10 unit preparation The volume (volume) is 200 mL ± 20%, the number is 2.0 × 10 ^{11 to} 2.9 × 10 ¹¹ (3) 15 unit preparation The volume (volume) is 250 mL ± 20%, the number is 3.0 × 10 ^{11 to} 3.9 × 10 ¹¹ (4) 20 unit preparation The volume (volume) is 250 mL ± 20%, and the number is 4.0 × 10 ¹¹ or more.

  Next, the action (operation) of the blood component collection device 1, that is, the platelet collection operation (blood component collection operation) using the blood component collection device 1 will be described with reference to the flowcharts shown in FIGS. explain.

  In the present embodiment, the blood component collection device 1 repeats the platelet collection operation (blood component collection operation) a plurality of times (1st cycle to nth cycle, n is an integer of 2 or more) under the control of the control unit 3. It is like that. This platelet collection operation will be described in detail later.

  In parallel with the final-cycle platelet collection operation or after completion of the final-cycle platelet collection operation, the blood component collection device 1 is temporarily collected in the intermediate bag 27a under the control of the control unit 3. Concentrated platelets (stored) are supplied to a leukocyte removal filter 261, and filtration of concentrated platelets, that is, a filtration operation (filtration step) for separating and removing leukocytes in the concentrated platelets is performed.

  In this filtering operation, the second flow path opening / closing means 82 is opened. Thereby, the concentrated platelets in the intermediate bag 27a are transferred into the platelet collection bag 26 through the tubes 46 and 47, the leukocyte removal filter 261 and the tube 48 due to a drop (self-weight). At this time, most of the concentrated platelets pass through the filtration member of the leukocyte removal filter 261, but the leukocytes are captured by the filtration member. For this reason, the removal rate of leukocytes in the platelet preparation can be made extremely high.

  The timing for starting this filtration operation is not particularly limited, but from the viewpoint of shortening the donor's restraint time, this filtration operation is performed simultaneously with the platelet collection operation of the final cycle (particularly in the early stages of the platelet collection operation). It is preferable to start. In the blood component collection device 1 of this embodiment, the filtration operation is started almost simultaneously with the start of the second plasma collection step in the platelet collection operation in the final cycle (for example, before step S105 in FIG. 3). Although configured, in the flowcharts shown in FIGS. 3 and 4, the description of the step of the filtration operation start (filtration process start) is omitted.

  The transfer of the concentrated platelets from the intermediate bag 27a to the platelet collection bag 26 may be performed using a pump.

[0] First, the blood collection needle 29 of the third line 23 and the first line 21 to the chamber 21d is primed with an anticoagulant, and then the blood collection needle 29 is placed in the blood vessel of the donor (blood donor). Puncture.
[1] Platelet collection operation in the first cycle (see FIGS. 3 and 4)
[11] First, the blood component collection device 1 performs a first plasma collection step. In the first plasma collection step, blood is introduced into the blood storage space 146 of the rotor 142 and plasma (PPP) separated by centrifuging the blood is collected in the plasma collection bag 25.

  In the first plasma collection step, first, the control unit 3 collects plasma (step S101 in FIG. 3).

  Specifically, under the control of the control unit 3, the first liquid supply / closing means 81 and the fifth flow path opening / closing means 85 are opened, and the other liquid flow opening / closing means are closed. The pump 11 is operated (forward rotation) at a predetermined rotational speed (preferably about 250 mL / min or less, more preferably about 40 to 150 mL / min, for example, 60 mL / min), and blood collection from the donor is started.

  Simultaneously with this blood collection, the third liquid feeding pump 13 is operated under the control of the control unit 3, and an anticoagulant such as an ACD-A liquid is supplied through the third line 23, This anticoagulant is mixed into the collected blood.

  At this time, the rotation speed of the third liquid feeding pump 13 is controlled by the control unit 3 so that the anticoagulant is mixed with the blood sample at a predetermined ratio (preferably about 1/20 to 1/6, for example 1/10) To be controlled.

  As a result, blood (anticoagulant-added blood) is transferred through the first line 21 and introduced into the blood storage space 146 of the rotor 142 through the tube 141 from the inlet 143 of the centrifuge 20.

  At this time, air (sterilized air) in the centrifuge 20 is sent into the air bag 27b through the second line 22 and the tube 42.

  At the same time as or before or after the blood collection, the control unit 3 operates the centrifuge drive device 10 to control the rotor 142 to rotate at a predetermined rotational speed.

  By the rotation of the rotor 142, the blood introduced into the blood storage space 146 is separated from the inside into three layers: a plasma layer (PPP layer) 131, a buffy coat layer (BC layer) 132, and a red blood cell layer (CRC layer) 133. The

  The rotational speed of the rotor 142 is preferably about 3000 to 6000 rpm, more preferably about 4200 to 5800 rpm. In the following steps, the control unit 3 does not change the rotational speed of the rotor 142 unless otherwise specified.

  Further, when the blood collection and the supply of the anticoagulant are continued and blood (about 270 mL) exceeding the capacity of the blood storage space 146 is introduced into the blood storage space 146, the blood storage space 146 is filled with blood and centrifuged. Plasma (PPP) flows out from the outlet 144 of the vessel 20.

  At this time, the bubble sensor 34 installed in the second line 22 detects that the fluid flowing in the second line 22 has changed from air to plasma, and the control unit 3 detects the bubble sensor 34. Based on the signal, control is performed so that the fifth flow path opening / closing means 85 is closed and the third flow path opening / closing means 83 is opened.

  As a result, plasma is introduced and collected into the plasma collection bag 25 via the second line 22 and the tubes 43 and 44.

  The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 3.

  The control unit 3 determines whether a predetermined amount (preferably about 10 to 50 g, for example, 30 g) of plasma has been collected in the plasma collection bag 25 based on information (weight signal) from the weight sensor 16, When a predetermined amount of plasma is collected in the plasma collection bag 25, the plasma in the plasma collection bag 25 is circulated through the blood storage space 146 at a constant speed.

  Specifically, the second liquid feeding pump 12 is operated (forward rotation) at a predetermined rotation speed (preferably about 5 to 105 mL / min, for example, 55 mL / min) under the control of the control unit 3.

  Thereby, the plasma in the plasma collection bag 25 is introduced into the blood storage space 146 through the plasma circulation line 24 and the first line 21 at a constant speed, and the plasma flowing out from the discharge port 144 of the centrifuge 20 is removed. It collects in the plasma collection bag 25 via the second line 22 and tubes 43 and 44. That is, the plasma in the plasma collection bag 25 is circulated in the blood storage space 146 at a constant speed.

  Next, the control unit 3 determines whether or not a predetermined amount of plasma has been collected in the plasma collection bag 25 based on information (weight signal) from the weight sensor 16 (step S102 in FIG. 3).

  The amount of plasma collected (predetermined amount) is preferably about 10 to 50 g, more preferably about 20 to 40 g.

  In step S102, when a predetermined amount of plasma is not collected in the plasma collection bag 25, the control unit 3 returns to step S101 and repeats step S101 and subsequent steps again.

  In step S102, when a predetermined amount of plasma is collected in the plasma collection bag 25, the control unit 3 ends this step [11] (first plasma collection step), and the first step Transition to constant-speed plasma circulation process.

[12] Next, the blood component collection device 1 performs a first constant-speed plasma circulation step. In the first constant-speed plasma circulation step, blood is introduced into the blood storage space 146 of the rotor 142, and the operation of collecting the plasma separated by centrifuging the blood into the plasma collection bag 25 is continued. The circulation speed (circulation amount) into the blood storage space 146 is made larger than that in the first plasma collection step, and the plasma in the plasma collection bag 25 is circulated through the blood storage space 146 at a constant speed.

  In the first constant-speed plasma circulation process, the control unit 3 collects the plasma into the collection bag 25, and the second sending is performed when the first plasma collection process shifts to the first constant-speed plasma circulation process. The rotation speed of the liquid pump 12 is increased (changed) to circulate plasma (step S103 in FIG. 3).

  Specifically, under the control of the control unit 3, the rotation speed of the second liquid feeding pump 12 is increased to a predetermined rotation speed (preferably about 55 to 225 mL / min, for example, 165 mL / min), and the second feeding pump 12 is increased. The liquid pump 12 is operated (forward rotation).

  Next, the control unit 3 determines whether or not a predetermined time (preferably about 10 to 90 seconds, for example, 30 seconds) has elapsed since the start of the first constant-speed plasma circulation (step S104 in FIG. 3). .

  If the predetermined time has not elapsed since the start of the first constant-speed plasma circulation in step S104, the control unit 3 returns to step S103 and repeats step S103 and subsequent steps again.

  In step S104, when a predetermined time has elapsed since the start of the first constant-speed plasma circulation, the control unit 3 ends the process [12] (first constant-speed plasma circulation process). Then, the process proceeds to the second plasma collection step.

[13] Next, the blood component collection device 1 performs a second plasma collection step. In the second plasma collection step, the circulation rate of plasma into the blood storage space 146 is made lower than that in the first constant-speed plasma circulation step, and the plasma in the plasma collection bag 25 is passed through the blood storage space 146 to be fixed. While continuing the operation of circulating at high speed, blood is introduced into the blood storage space 146 of the rotor 142, and the plasma separated by centrifuging the blood is collected in the plasma collection bag 25.

  In the second plasma collection step, instead of measuring the amount of plasma collected by the weight sensor 16, the process is terminated when the position of the interface B between the plasma layer 131 and the buffy coat layer 132 is detected. The same step as step [11] (first plasma collecting step) is performed.

  In the second plasma collection process, the control unit 3 collects the plasma into the collection bag 25, and moves to the second plasma collection process from the first constant-speed plasma circulation process. The rotational speed of 12 is reduced (changed) to circulate plasma (step S105 in FIG. 3).

  Thereby, as the amount of red blood cells in the blood storage space 146 increases, that is, as the layer thickness of the red blood cell layer 133 increases, the interface B gradually moves in the direction of the rotation axis of the rotor 142.

  Next, the control unit 3 determines whether or not the interface B has reached a predetermined level (first position) based on the detection signal (interface position detection information) from the optical sensor 15 (step S106 in FIG. 3). ).

  The first position of the interface B is preferably the position at which the detection signal from the first optical sensor 15 (output voltage from the light receiving unit 152) is about 1 to 2V. .

  If the interface B has not reached the first position in step S106, the control unit 3 returns to step S105 and repeats step S105 and subsequent steps again.

  In step S106, when the interface B reaches the first position, the control unit 3 ends this step [13] (second plasma collection step) and performs the second constant-speed plasma circulation. Move to the process.

[14] Next, the blood component collection device 1 performs the second constant-speed plasma circulation step. In the second constant-speed plasma circulation step, blood is introduced into the blood storage space 146 of the rotor 142, and the operation of collecting the plasma separated by centrifuging the blood into the plasma collection bag 25 is continued. The circulation speed into the blood storage space 146 is made larger than that in the second plasma collection step, and the plasma in the plasma collection bag 25 is circulated through the blood storage space 146 at a constant speed.

  In the second constant-speed plasma circulation step, the control unit 3 collects the plasma into the collection bag 25, and moves to the second constant-speed plasma circulation step when shifting from the second plasma collection step to the second constant-speed plasma circulation step. The rotation speed of the liquid pump 12 is increased (changed) to circulate plasma (step S107 in FIG. 3).

  Specifically, under the control of the control unit 3, the rotation speed of the second liquid feeding pump 12 is increased to a predetermined rotation speed (preferably about 10 to 175 mL / min, for example, 85 mL / min), and the second liquid supply pump 12 is increased. The liquid pump 12 is operated (forward rotation).

  Next, the control unit 3 determines whether or not a predetermined time (preferably about 10 to 90 seconds, for example, 30 seconds) has elapsed since the start of the second constant-speed plasma circulation (step S108 in FIG. 3). .

  In step S108, when the predetermined time has not elapsed since the start of the second constant-speed plasma circulation, the control unit 3 returns to step S107 and repeats step S107 and subsequent steps again.

  In step S108, when a predetermined time has elapsed since the start of the second constant-speed plasma circulation, the control unit 3 ends this step [14] (second constant-speed plasma circulation step). Then, the process proceeds to the third plasma collection step.

[15] Next, the blood component collection device 1 performs a third plasma collection step. In the third plasma collection step, the circulation rate of plasma into the blood storage space 146 is made lower than that in the second constant-speed plasma circulation step, and the plasma in the plasma collection bag 25 is passed through the blood storage space 146 to be fixed. While continuing the operation of circulating at high speed, blood is introduced into the blood storage space 146 of the rotor 142, and the plasma separated by centrifuging the blood is collected in the plasma collection bag 25.

  Next, the control unit 3 determines whether or not a predetermined amount of plasma has been collected in the plasma collection bag 25 based on the amount and the number of rotations per rotation of the first solution pump 11 (FIG. 3). Step S110).

  The amount of plasma collected (predetermined amount) is preferably about 2 to 30 g, more preferably about 5 to 15 g.

  When a predetermined amount of plasma is collected in the plasma collection bag 25 in step S110, the control unit 3 ends this step [15] (third plasma collection step), and the platelet collection step. (Transition to 1 in FIG. 4).

[16] Next, blood component collection device 1 performs a platelet collection step. In the platelet collection step, the collection of plasma in the collection bag 25 is interrupted, and the plasma in the plasma collection bag 25 is circulated in the blood storage space 146 while being accelerated at the first acceleration, and then the first acceleration is performed. The second acceleration is changed to a larger second acceleration, and is circulated while being accelerated at the second acceleration. The platelets are discharged from the blood storage space 146, and the concentrated platelets are collected (stored) in the intermediate bag 27a.

  In the platelet collection step, first, the control unit 3 stops collecting plasma into the collection bag 25 and performs plasma circulation by the first acceleration (step S111 in FIG. 4).

  Specifically, under the control of the control unit 3, the first flow path opening / closing means 81 is closed, the first liquid feed pump 11 and the third liquid feed pump 13 are stopped, and the second liquid feed pump. 12 is operated (forward rotation) so that its rotation speed increases (increases) at the first acceleration.

  Thereby, the blood collection is interrupted, and the plasma in the plasma collection bag 25 is introduced into the blood storage space 146 through the plasma circulation line 24 and the first line 21 while being accelerated at the first acceleration, and then centrifuged. Plasma flowing out from the outlet 144 of the vessel 20 is collected in the plasma collection bag 25 via the second line 22 and the tubes 43 and 44. That is, the plasma in the plasma collection bag 25 is circulated in the blood storage space 146 while being accelerated at the first acceleration.

  At this time, if plasma is circulated in the blood storage space 146 while accelerating at the first acceleration, the red blood cell layer 133 is diffused (increase in layer thickness), and the interface B gradually moves in the direction of the rotation axis of the rotor 142 To do.

  The first acceleration is preferably about 0.5 to 10 mL / min / sec, more preferably about 1.5 to 2.5 mL / min / sec. The first acceleration may not be constant, and may change stepwise or continuously within the above range, for example.

  The initial speed of the first liquid delivery pump 11 in the plasma circulation by the first acceleration is preferably about 40 to 150 mL / min, more preferably about 50 to 80 mL / min.

  Next, the control unit 3 continues step S111 until the circulating speed of plasma into the blood storage space 146 reaches a predetermined speed (step S112 in FIG. 4).

  The predetermined speed, that is, the rotational speed of the first liquid delivery pump 11 when the plasma circulation by the first acceleration is completed is preferably about 100 to 180 mL / min, more preferably 140 to 160 mL / min. About min.

  In step S112, when the circulation speed of the plasma into the blood storage space 146 reaches a predetermined speed, the control unit 3 performs plasma circulation by the second acceleration (step S113 in FIG. 4).

  Specifically, under the control of the control unit 3, the acceleration of the second liquid feed pump 12 is changed from the first acceleration to the second acceleration, and the rotation speed of the second liquid feed pump 12 is changed. Actuate (forward) to increase (increase) at the second acceleration. Thereby, the plasma in the plasma collection bag 25 is circulated in the blood storage space 146 while being accelerated by the second acceleration.

  At this time, if plasma is circulated in the blood storage space 146 while accelerating at the second acceleration, diffusion of the red blood cell layer 133 (increase in layer thickness) occurs, and the interface B gradually moves in the direction of the rotation axis of the rotor 142 At the same time, the platelets in the buffy coat layer 132 float against the centrifugal force (float up) and move toward the discharge port 144 of the rotor 142.

  The second acceleration is set to be greater than the first acceleration, and is preferably about 3 to 20 mL / min / sec, more preferably about 5 to 10 mL / min / sec. Note that the second acceleration may not be constant, and may change stepwise or continuously within the above range, for example.

  Next, the control unit 3 determines whether or not the circulating speed of plasma into the blood storage space 146 has reached a predetermined speed, that is, the rotational speed of the first liquid feeding pump 11 is a predetermined speed (preferably 120 to 300 mL / min). It is determined whether or not a certain level (for example, 250 mL / min) has been reached (step S114 in FIG. 4).

  In step S114, when the circulation speed of plasma into the blood storage space 146 has not reached the predetermined speed, the control unit 3 returns to step S113 and repeats step S113 and subsequent steps again.

  In step S114, when the circulation speed of plasma into blood storage space 146 reaches a predetermined speed, control unit 3 continues the plasma circulation (step S115 in FIG. 4).

  Specifically, the control unit 3 controls to maintain (hold) the rotation speed of the second liquid feeding pump 12 at the predetermined speed in step S114. Thereby, the circulation speed of plasma into the blood storage space 146 is preferably about 120 to 300 mL / min, for example, 250 mL / min.

  Next, the control unit 3 determines whether or not a predetermined time (preferably about 5 to 15 seconds, for example, 10 seconds) has elapsed since the start of Step S115 (Step S116 in FIG. 4).

  In step S116, when the predetermined time has not elapsed since the start of step S115, the control unit 3 then determines that the output voltage (PC concentration voltage) from the turbidity sensor 14 is a predetermined value (preferably 2. It is determined whether or not the voltage has decreased to about 5 to 3.5 V, for example, 3.0 V (step S117 in FIG. 4).

  In step S117, if the output voltage from the turbidity sensor 14 has not decreased below the predetermined value, the control unit 3 returns to step S115 and repeats step S115 and subsequent steps again.

  While repeating steps S115 to S117, in step S116, when a predetermined time has elapsed since the start of step S115, the control unit 3 ends this step [16] (platelet collection step). Then, the process proceeds to step S122 described later.

  In step S117, when the output voltage from the turbidity sensor 14 decreases to a predetermined value or less, that is, as platelets flow out from the discharge port 144 of the rotor 142, the second line 22 flows. When the platelet concentration in plasma reaches a predetermined value or more, the control unit 3 collects platelets (PC) (step S118 in FIG. 4).

  Specifically, based on the detection signal of the turbidity sensor 14, the control unit 3 controls the third flow path opening / closing means 83 to be closed and the fourth flow path opening / closing means 84 to be opened.

  Thereby, concentrated platelets are introduced into the intermediate bag 27a via the second line 22 and the tubes 43 and 45, and collected (stored). At this time, since the second flow path opening / closing means 82 is closed, concentrated platelets do not flow out of the intermediate bag 27a.

  Further, the control unit 3 calculates the platelet concentration (cumulative PC concentration) in the intermediate bag 27a based on the output voltage (detection signal) from the turbidity sensor 14. The platelet concentration continues to rise after starting the collection of platelets, and once it reaches the maximum concentration, it begins to fall.

  Next, the control unit 3 determines whether or not a predetermined time (preferably about 10 to 25 seconds, for example, 15 seconds) has elapsed since the start of platelet collection (step S119 in FIG. 4).

  If the predetermined time has not elapsed since the start of platelet collection in step S119, the control unit 3 then determines whether the output voltage (PC concentration voltage) of the turbidity sensor 14 has reached a predetermined value or less. It is determined whether or not (step S120 in FIG. 4).

  The predetermined value of the output voltage of the turbidity sensor 14 is a value near the time when red blood cells are mixed in the plasma flowing in the second line 22, and is preferably about 0.5 V or less.

  In step S120, when the output voltage of the turbidity sensor 14 has not reached the predetermined value or less, the control unit 3 then determines whether or not the concentrated platelets in the intermediate bag 27a have reached a predetermined amount. (Step S121 in FIG. 4).

  The collection amount (predetermined amount) of the concentrated platelets is preferably about 20 to 100 mL, more preferably about 30 to 80 mL.

  In step S121, when the concentrated platelets in the intermediate bag 27a do not reach the predetermined amount, the control unit 3 returns to step S118 and repeats step S118 and subsequent steps again.

  While repeating steps S118 to S121, when a predetermined time has elapsed since the start of platelet collection in step S119, or the output voltage of the turbidity sensor 14 has reached a predetermined value or less in step S120. In this case, the control unit 3 ends this step [16] (platelet collection step), and proceeds to step S122 described later.

  Further, when a predetermined time has elapsed in steps S116 and S119, when the output voltage (PC concentration voltage) of the turbidity sensor 14 is equal to or lower than a predetermined value in step S120, the concentrated platelets in the intermediate bag 27a in step S121. Reaches the predetermined amount, the control unit 3 opens the fifth flow path opening / closing means 85 and closes all the other flow path opening / closing means 81 to 84, 86, The liquid feeding pump 12 is stopped, and this step [16] (platelet collecting step) is completed.

[17] Next, the blood component collection device 1 performs a step of stopping the centrifuge 20.
In this step, first, the control unit 3 decelerates the centrifuge 20 (step S122 in FIG. 4).

Specifically, under the control of the control unit 3, the rotational speed of the centrifuge drive device 10 is decreased and the rotor 142 is decelerated.
Further, the control unit 3 stops the centrifuge 20 (step S123 in FIG. 4).

  Specifically, under the control of the control unit 3, the rotation of the centrifuge drive device 10 is stopped and the rotor 142 is stopped.

[18] Next, the blood component collection device 1 performs a blood return step. In the blood return process, blood components (remaining blood components) in the blood storage space 146 of the rotor 142 are returned.
In the blood return process, the control unit 3 performs blood return (step S124 in FIG. 4).

  Specifically, under the control of the control unit 3, the first flow path opening / closing means 81 and the fifth flow path opening / closing means 85 are opened, and the first liquid feed pump 11 is rotated at a predetermined rotational speed (preferably 20). Operates (reverses) at about 120 mL / min, for example, 90 mL / min.

  Thereby, blood components (mainly red blood cells and white blood cells) remaining in the blood storage space 146 of the rotor 142 are discharged from the inflow port 143 of the centrifuge 20 and are passed through the first line 21 (blood collection needle 29). Blood is returned (returned) to the donor.

Then, after the air discharged from the centrifugal separator 20 is detected by the bubble sensor 32 and the first liquid feeding pump 11 is rotated a predetermined number of times, the first flow path opening / closing means 81 and the fifth flow path are While closing the opening / closing means 85, the first liquid pump 11 is stopped, and this step [18] (blood returning step) is completed.
Thereby, the platelet collection operation in the first cycle is completed.

[2] Platelet collection operation in the second cycle that is not the final cycle (see FIGS. 3 and 4)
Subsequently, the platelet collection operation of the second cycle is performed.

  In the platelet collecting operation in the second cycle, the same steps as the platelet collecting operation in the first cycle are performed as follows.

[21] to [28] Steps similar to the steps [11] to [18] are performed, respectively.
Thereby, the platelet collection operation in the second cycle is completed.
The same applies to the platelet collection operation after the third cycle which is not the final cycle.

[3] Platelet collection operation in the final cycle (see FIGS. 3 and 4)
Subsequently, the platelet collection operation for the final cycle is performed.

  In the platelet collection operation in the final cycle, a filtration operation (filtration process) for separating and removing leukocytes from the concentrated platelets is performed, and air discharged from the centrifuge 20 is detected by the bubble sensor 32 when returning blood to further detect the bubble sensor. If air is detected at 35 or 36, the blood return process is terminated, and the same process as the platelet collection operation in the first cycle is performed.

[31] to [37] Steps similar to the steps [11] to [17] are performed except that the filtration operation is performed.

[38] The air discharged from the centrifugal separator 20 is detected by the bubble sensor 35 or 36 to close the first flow path opening / closing means 81 and the fifth flow path opening / closing means 85, and the first liquid feed Except for stopping the pump 11 and ending this step [38] (blood return step), the same step as the step [18] is performed.
Thereby, the platelet collection operation in the final cycle is completed.

  The platelet collection operation is not limited to being performed a plurality of times, and may be performed only once, for example.

  The configuration of the blood component collection circuit 2 can also be set as appropriate, and is not limited to the illustrated configuration.

  As described above, according to this blood component collection device, not only the first plasma collection step, the second plasma collection step, and the third plasma collection step, but also the first plasma circulation step and the second plasma are collected. Even in the circulation process, the first liquid pump 11 operates to collect blood from the donor, introduce the blood into the blood storage space 146 of the centrifuge 20 and separate it, and plasma is collected in the plasma collection bag 25. Since the blood is collected, the blood collection time can be shortened. Thereby, the occupation time of the blood component collection device 1 can be reduced, and the burden on the donor can be reduced.

  In addition, in each of the transition from the first plasma collection process to the first constant-speed plasma circulation process and the transition from the second plasma collection process to the second constant-speed plasma circulation process, the second feeding is performed. The flow rate of the liquid component introduced into the blood storage space 146 in each constant-speed plasma circulation step (plasma circulation flow rate) is increased by increasing the liquid feed amount of the liquid pump 12, and the liquid introduced into the blood storage space 146 in the plasma collection step Since it is larger than the flow rate of the sex component (circulation flow rate of plasma), platelets trapped by the separated red blood cell layer can be surely washed out, and excessive increase in the internal viscosity of the buffy coat layer (buffy coat layer) Excessive concentration) can be prevented (blocked), thereby improving the recovery rate (yield) of platelets.

  As mentioned above, although the blood component collection device of the present invention has been described based on the illustrated embodiment, the present invention is not limited to this, and the configuration of each part is an arbitrary configuration having the same function. Can be replaced. Moreover, other arbitrary structures and processes may be added to the present invention.

  In addition, the blood component collection device of the present invention is not limited to the application to obtain both platelet preparations and plasma preparations (or the raw material plasma of plasma fractionation preparations), but only to obtain platelet preparations from blood. You may apply.

  Moreover, the blood component collection device of the present invention is not limited to the application to obtain a platelet preparation or a plasma preparation, and may be applied to obtain an erythrocyte preparation, a leukocyte preparation, etc. from blood, for example. That is, in the blood component collection device of the present invention, the blood cell component collected in the blood component collection bag is not limited to platelets (platelets including plasma), but, for example, red blood cells (red blood cells including plasma), white blood cells (white blood cells including plasma) Or the like.

  In the present invention, the blood separator is not limited to the centrifugal type, and may be, for example, a membrane type.

  In the present invention, the cells separated and removed by the cell separation filter (filter) are not limited to leukocytes.

  In the present invention, the optical sensor is not limited to the illustrated one, and may be a line sensor, for example.

  Moreover, the system of the blood component collection device of the present invention is not limited to the intermittent system, and may be, for example, a continuous system. Moreover, the thing without a blood return process may be sufficient.

  According to the present invention, since the flow rate of the liquid component introduced into the blood separator is relatively large, that is, the plasma circulation step is set to be larger than the flow rate in the plasma collection step, Thus, blood cell components (eg, platelets) to be collected that are confined by the separated erythrocyte layer can be reliably washed out. Thereby, the recovery rate of the blood cell component can be improved. In the plasma circulation process, blood is collected from a donor and collected in a plasma collection bag, so that the blood collection time can be shortened. Thereby, the occupation time of the blood component collection device can be reduced, and the burden on the blood donor can be reduced. Therefore, it has industrial applicability.

Claims (9)

A blood collection means comprising a hollow needle for collecting blood from a donor,
A blood separator for separating blood collected by the blood collecting means;
A plasma collection bag for collecting plasma separated by the blood separator;
A blood component collection bag for collecting predetermined blood cell components separated by the blood separator;
A blood treatment line connecting the hollow needle and the inlet of the blood separator;
A blood component collection circuit comprising a plasma circulation line branched from a branch portion provided in the blood treatment line and connected to the plasma collection bag;
A first liquid feeding means installed in the blood processing line and for feeding at least a fluid in the blood processing line;
A second liquid feeding means that is installed in the plasma circulation line and feeds at least the plasma collected in the plasma collection bag;
The collected blood is separated and collected in the plasma collection bag by the plasma collection step of collecting plasma in the plasma collection bag by the operation of the first liquid feeding means and the operation of the second liquid delivery means. Blood component that collects blood by executing a plasma circulation step of circulating the collected plasma to the blood separator via a plasma circulation line and a blood component collection step of collecting a predetermined blood cell component in the blood component collection bag A collecting device,
When shifting from the plasma collection step to the plasma circulation step, the liquid supply amount of the second liquid supply unit is increased while continuing the operation of the first liquid supply unit, whereby the plasma circulation step In this case, while collecting the plasma in the plasma collection bag, the plasma collected in the plasma collection bag is circulated to the blood separator via the plasma circulation line, and the liquid component introduced into the blood separator The blood component collection device is configured to make the flow rate of the blood flow larger than the flow rate of the liquid component introduced into the blood separator in the plasma collection step.   The blood component collection device according to claim 1, wherein the flow rate of the liquid component introduced into the blood separator in the plasma circulation step is 40 to 250 mL / min.   The difference between the flow rate of the liquid component introduced into the blood separator in the plasma circulation step and the flow rate of the liquid component introduced into the blood separator in the plasma collection step is 10 to 220 mL / min. The liquid component collection device according to claim 1.   The plasma collecting step is configured such that the second liquid feeding means is operated to circulate the plasma collected in the plasma collection bag to the blood separator via the plasma circulation line. The blood component collecting apparatus according to claim 1 in the range.   In the plasma collection step, the operation of the second liquid feeding means is controlled so that the flow rate of the liquid component introduced into the blood separator becomes a predetermined target value. The blood component collection device according to claim 1, wherein the blood component collection device is configured to adjust a liquid feeding amount.   In the plasma circulation step, the operation of the second liquid feeding means is controlled so that the flow rate of the liquid component introduced into the blood separator becomes a predetermined target value. The blood component collection device according to claim 1, wherein the blood component collection device is configured to adjust a liquid feeding amount.   The blood plasma collection step is performed a plurality of times before the blood component collection step, and the plasma circulation step is performed between the plasma collection steps. The blood component collection device described.   The blood component collection device has at least one cycle of blood component collection operation including the plasma collection step, the plasma circulation step, the blood component collection step, and a blood return step of returning the remaining blood components to a donor. The blood component collection device according to claim 1, which is to be executed. The blood component collection apparatus according to claim 1, wherein the predetermined blood cell component is platelets.

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