JP2006015072A - Blood component collecting apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a blood component collecting apparatus capable of automatically determining whether a blood sampling and returning line is correctly mounted to a predetermined detecting means for detecting gas and liquid in the line. <P>SOLUTION: This blood component collecting apparatus 1 is provided with bubble sensors 35, 36 mounted with the blood sampling and returning line 21, and a bubble sensor 31 mounted with an anticoagulant injection line 23. When performing priming operation of injecting an anticoagulant from the other end of the anticoagulant injection line 23 by the operation of an anticoagulant liquid feed pump 12 to charge a part of the blood sampling and returning line 21 and the anticoagulant injection line 23 with the anticoagulant prior to the start of blood sampling, in the case that the anticoagulant detected by the bubble sensor 35 or 36 before the liquid feed quantity of the anticoagulant liquid feed pump 12 from the time of detecting the anticoagulant by the bubble sensor 31 reaches a first predetermined quantity, it is determined that the anticoagulant injection line 23 is erroneously mounted to the bubble sensors 35, 36. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a blood component collection device.

  When collecting blood, the collected blood is separated into each blood component by centrifugation, etc. for reasons such as effective use of the blood and reduction of the burden on the donor, and only the components necessary for the transfuser are collected. Blood samples are returned to the donor (donor).

  In such component blood collection, for example, when obtaining a platelet preparation, blood collected from a blood donor is introduced into a blood component collection circuit, and a centrifuge called a centrifuge bowl installed in the blood component collection circuit is used to obtain plasma, buffy It is separated into a coat and red blood cells, and platelets (predetermined blood components) are separated from the buffy coat, and collected in a container to form a platelet preparation. The remaining plasma, white blood cells and red blood cells can be returned to the donor. (For example, refer to Patent Document 1).

  In the device used for collecting blood components, there is an air bubble sensor that detects air bubbles in the blood collection / return line in order to prevent air bubbles from being injected into the body of the donor during blood return, and a blood collection / return line. Detection means for detecting gas and liquid such as a bubble sensor for confirming that the anticoagulant is supplied to the anticoagulant injection line for injecting the anticoagulant is provided at a plurality of locations. Since the blood component collection circuit is disposable, when blood is collected from a new blood donor, a new blood component collection circuit is set in the main body of the apparatus, and a tube of a predetermined line is attached to these bubble sensors. I do.

  However, when attaching a tube of a predetermined line to each bubble sensor, due to the mistake of the operator (nurse), the tube of the anticoagulant injection line is mistakenly attached to the bubble sensor to which the blood collection / return line tube should be attached. May be worn. If blood collection and blood return are performed without noticing this mistake in attachment, the air bubbles in the blood return may be missed without being detected, and there is a risk of air bubbles being injected into the body of the blood donor.

Japanese National Patent Publication No. 8-509403

  An object of the present invention is to provide a blood component collection device that can automatically determine whether or not a blood collection / return line is correctly attached to a predetermined detection means for detecting gas and liquid in the line. There is.

  Such an object is achieved by the present inventions (1) to (9) below. Moreover, it is preferable that it is following (10)-(12).

(1) A blood component collection device that centrifuges blood collected from a donor, collects a predetermined blood component, and returns the remaining blood component to the donor,
Blood collection means for collecting blood from a donor,
A centrifuge for centrifuging blood collected by the blood collection means;
A blood component collection bag for collecting a predetermined blood component separated by the centrifuge;
A blood collection / return line connecting the blood collection means and the centrifuge;
A blood component collection circuit having an anticoagulant injection line having one end connected in the middle of the blood collection / return line;
An anticoagulant feeding pump for feeding the anticoagulant of the anticoagulant injection line;
A first detection means attached to the blood collection / return line and detecting gas and liquid in the blood collection / return line;
A control unit for controlling the operation of the anticoagulant feeding pump,
The controller controls the anticoagulation until the anticoagulant is detected by the first detection means when the anticoagulant is fed from the other end of the anticoagulant injection line prior to the start of blood collection. A blood component collecting apparatus, wherein it is determined whether or not the blood collection / return line is attached to the first detection means based on an operation amount of a drug delivery pump.

  (2) The control unit measures the liquid feeding amount of the anticoagulant based on the operating amount of the anticoagulant liquid feeding pump, and the liquid feeding amount of the anticoagulant reaches a first predetermined amount. In the above (1), when the anticoagulant is detected by the first detection unit before the determination, it is determined that the anticoagulant injection line is erroneously attached to the first detection unit. Blood component collection device.

  (3) When the control unit determines that the anticoagulant injection line is mistakenly attached to the first detection means, the control unit stops the operation of the anticoagulant feeding pump. The blood component collection device described.

  (4) The control unit measures the liquid feeding amount of the anticoagulant based on the operating amount of the anticoagulant liquid feeding pump, and the liquid feeding amount of the anticoagulant reaches a first predetermined amount. Even if the anticoagulant is not detected by the first detection means, the anticoagulant liquid is continued to be fed, and the anticoagulant liquid feed amount is more than the first predetermined amount. When the anticoagulant is detected by the first detection means before reaching the second predetermined amount that is larger than the predetermined amount, it is determined that the blood collection / return line is attached to the first detection means. The blood component collection device according to any one of (1) to (3) above.

  (5) If the anticoagulant is not detected by the first detection means even if the liquid feeding amount of the anticoagulant reaches the second predetermined amount, the control unit collects the blood The blood component collection device according to (4), wherein it is determined that a blood return line is not attached to the first detection means.

(6) Further, a second detection that is mounted closer to the centrifuge than the first detection means mounted on the blood collection / return line, and detects gas and liquid in the blood collection / return line. With means,
The control unit measures the amount of the anticoagulant delivered based on the operation amount of the anticoagulant delivery pump, and before the amount of the anticoagulant delivered reaches a first predetermined amount. When the anticoagulant is detected by the first detection unit or the second detection unit, the anticoagulant injection line is erroneously attached to the first detection unit and / or the second detection unit. The blood component collection device according to any one of the above (1) to (5), which is judged to be performed.

(7) Furthermore, it is equipped with the 3rd detection means with which it mounts | wears with the said anticoagulant injection line, and detects the gas and liquid in this anticoagulant injection line,
The control unit measures the liquid feeding amount of the anticoagulant based on the operation amount of the anticoagulant liquid feeding pump, detects the anticoagulant by the third detection means, and then performs the first operation. (1) to (1) to determine whether the blood collection / return line is attached to the first detection means based on the amount of the anticoagulant delivered until the anticoagulant is detected by the detection means. (6) The blood component collection device according to any one of (6).

  (8) The controller is configured to determine a liquid feeding amount of the anticoagulant from the detection of the anticoagulant by the third detection unit to the detection of the anticoagulant by the first detection unit. If the anticoagulant is detected by the first detection means before the predetermined amount of 1 is reached, it is determined that the anticoagulant injection line is erroneously attached to the first detection means. The blood component collection device according to (7) above.

(9) Further, a second detection that is mounted closer to the centrifuge than the first detection means mounted on the blood collection / return line, and detects gas and liquid in the blood collection / return line. Means,
A third detection means mounted on the anticoagulant injection line and detecting gas and liquid in the anticoagulant injection line;
The control unit measures the liquid feeding amount of the anticoagulant based on the operation amount of the anticoagulant liquid feeding pump, detects the anticoagulant by the third detection means, and then performs the first operation. Before the detection of the anticoagulant by the detection means reaches the first predetermined amount, the anticoagulant by the first detection means or the second detection means. Any one of the above (1) to (5), in which it is determined that the anticoagulant injection line is erroneously attached to the first detection means and / or the second detection means The blood component collection device according to 1.

  (10) When the control unit detects the anticoagulant by the first detection unit or the second detection unit before the liquid feeding amount of the anticoagulant reaches the first predetermined amount The blood component collection device according to (6), wherein the operation of the anticoagulant feeding pump is stopped.

  (11) The control unit detects the anticoagulant by the first detection unit or the second detection unit even when the liquid feeding amount of the anticoagulant reaches the first predetermined amount. If there is not, the first detection means continues the liquid feeding of the anticoagulant and before the liquid feeding amount of the anticoagulant reaches a second predetermined amount larger than the first predetermined amount. Alternatively, when the anticoagulant is detected by the second detection means, it is determined that the blood collection / return line is attached to the first detection means and / or the second detection means The blood component collection device according to (6).

  (12) The control unit detects the anticoagulant by the first detection unit or the second detection unit even when the liquid feeding amount of the anticoagulant reaches the first predetermined amount. If not, the anticoagulant feeding is continued, and even if the anticoagulant feeding amount reaches the second predetermined amount, the first detecting means or the second detecting means When the anticoagulant is not detected, it is determined that the blood collection / return line is not attached to the first detection means and the second detection means. Component collection device.

  According to the present invention, it is possible to automatically determine whether or not the blood collection / return line is correctly attached to the predetermined detection means for detecting the gas and liquid in the line. Therefore, when the blood collection / return line is not attached to the predetermined detection means, the operator can be notified and correctly attached, so that the blood component collection device can be used safely.

  In particular, it is possible to detect whether or not the blood collection / return line is attached to a predetermined detection means during the priming operation, which is the stage before starting blood collection from the blood donor, so it is extremely safe, Excellent operability.

  Hereinafter, the blood component collection device of the present invention will be described in detail based on preferred embodiments shown in the accompanying drawings.

  FIG. 1 is a perspective view showing an embodiment of a blood component collection device of the present invention, FIG. 2 is a developed view of a blood component collection circuit in the blood component collection device shown in FIG. 1, and FIG. It is a fragmentary sectional view in the state where the centrifuge was equipped with the centrifuge drive device with which the blood component sampling device shown is shown.

  A blood component collection device 1 shown in FIGS. 1 and 2 is a device for separating blood into a plurality of blood components and collecting the separated blood components. The blood component collection device 1 includes a device body 3 and a blood component collection circuit (collection circuit) 2 that is detachably attached to the device body 3.

  As shown in FIG. 2, the blood component collection circuit 2 includes a rotor 142 having a blood storage space 146 therein, an inlet 143 and an outlet (outlet) 144 communicating with the blood storage space 146, A centrifuge 20 that centrifuges blood introduced from the inlet 143 by rotation in the blood storage space 146, a blood collection / return line that connects the blood collection needle (blood collection means) 29 and the inlet 143 of the centrifuge 20. 21, the collection bag side line 22 connected to the outlet 144 of the centrifuge 20, the anticoagulant injection line 23 connected to the blood collection / return line 21, and blood collection / return via the tubes 49 and 50. A plasma collection bag 25 connected to the blood line 21 and connected to the collection bag side line 22 via the tubes 43 and 44; and a collection bag side line via the tube 42. 22, connected to the collection bag side line 22 via the tubes 43 and 45, and connected to the intermediate bag 27 a via the tubes 46, 47 and 48. A platelet collection bag 26, and a bag 28 connected to the platelet collection bag 26 through a tube 51.

  The apparatus body 3 includes a centrifuge drive device 10 for rotating the rotor 142 of the centrifuge 20, a first liquid feeding pump 11 for blood collection / return line 21, and an anticoagulant injection line. And a plurality of (in the present embodiment, first to seventh seven pumps) that can open and close the middle of the flow path of the second liquid pump (anticoagulant liquid pump) 12 and the blood component collection circuit 2 ) Channel opening / closing means 81, 82, 83, 84, 85, 86, 87, centrifuge drive device 10, first liquid feeding pump 11, second liquid feeding pump 12, and a plurality of channel opening / closing means. A control unit (control means) 13 for controlling 81 to 87, a turbidity sensor (platelet concentration sensor) 14, an optical sensor 15, a weight sensor 16, and a plurality (six in this embodiment). With bubble sensors 31, 32, 33, 34, 35, 36 .

  The blood component collection circuit 2 connects a blood collection needle (blood collection means) 29 for collecting blood from a blood donor (donor) and an inlet 143 of the centrifuge 20 and collects and returns blood with a first pump tube 21g. The second pump tube is connected to the line 21, the collection bag side line 22 whose one end is connected to the discharge port (outlet) 144 of the centrifuge 20, and the blood collection needle 29 of the blood collection / return line 21. An anticoagulant injection line 23 provided with 23 a, a tube 50 connected to the blood collection needle 29 side from the pump tube 21 g of the blood collection / return line 21, a tube 49 connected to the tube 50, and a collection bag side line 22 The connected tube 43, the tube 44 connected to the tube 43, the plasma collection bag 25 connected to the tubes 44 and 49, and the collection bag side line 22 A connected tube 42, an air bag 27b connected to the tube 42, a tube 45 connected to the tube 43, an intermediate bag 27a connected to the tube 45, a tube 46 connected to the intermediate bag 27a, A tube 47 connected to the tube 46; a tube 48; a platelet collection bag 26 connected to the tube 48; a tube 51 connected to the platelet collection bag 26; and a bag 28 connected to the tube 51. Yes. The air bag 27b and the intermediate bag 27a are integrally formed (integrated).

  The blood collection / return line 21 includes a blood collection needle side line 21a to which a blood collection needle 29 is connected, a centrifugal separation in which one end side is connected to the blood collection needle side line 21a and the other end side is connected to the inlet 143 of the centrifuge 20. And a container side line 21b. As the blood collection needle 29, for example, a known metal needle is used.

  Each of the blood collection needle side line 21a, the centrifuge side line 21b, the collection bag side line 22 and the anticoagulant injection line 23 described later is formed by connecting a plurality of soft resin tubes or soft resin tubes. Has been.

  The blood collection needle side line 21a is connected from the blood collection needle 29 side to the branch connector 21c for connection to the anticoagulant injection line 23, the chamber 21d for removing bubbles and microaggregates, and the branch connector 21f for connection to the tube 50. And.

  In addition, a bubble sensor 35 (first detection means), a bubble sensor 36 (second detection means), and a bubble sensor 32 installed in the apparatus main body 3 are provided on the tube of the blood collection needle side line 21a. It is installed in this order from the side. In this case, the bubble sensors 35 and 36 are disposed between the branch connector 21c and the chamber 21d, and the bubble sensor 32 is disposed between the chamber 21d and the branch connector 21f.

  As shown in FIG. 1, the bubble sensors 35 and 36 are installed side by side on the front surface of the apparatus body 3. The bubble sensor 32 is installed side by side with the bubble sensor 31 on the front side of the upper surface of the apparatus main body 3.

  These bubble sensors 35, 36 and 32 include a housing having a shape in which a cylinder is divided into a vertical half through a groove, and an ultrasonic transmission unit provided on one side across the groove of the housing. And an ultrasonic wave receiving unit provided on the other side. When the tube is inserted into the groove of the housing, the tube is held (clamped) in the groove, and the tube is configured to be in close contact with the ultrasonic transmission unit and the ultrasonic reception unit. The bubble sensors 35, 36, and 32 transmit and receive ultrasonic waves from the outside of the tube, and make use of the fact that the transmittance of ultrasonic waves differs between the liquid and the bubble, so that the gas and liquid in the tube (separate gas / liquid) Can be detected. Therefore, each bubble sensor can detect whether the liquid has arrived at the time of priming, the presence or absence of bubbles in the tube, and the like.

  In the blood component collecting apparatus 1, by providing the bubble sensors 35 and 36, when returning blood, it is possible to monitor the presence or absence of bubbles in the blood collection needle side line 21a so as not to inject bubbles into the body of the blood donor. . In particular, in the present embodiment, by providing the two bubble sensors 35 and 36, double check can be performed, and the presence or absence of bubbles in the blood collection needle side line 21a can be monitored more accurately. .

  The bubble sensors 31, 33 and 34 also have the same function as described above. In the present invention, the bubble sensor is not limited to the ultrasonic sensor, and an optical sensor, an infrared sensor, or the like may be used.

  As shown in FIG. 2, an air-permeable and bacteria-impermeable filter 21i is connected to the chamber 21d through a tube 21h. This line can be used, for example, for detecting the internal pressure of the blood collection needle side line 21a.

  On the other hand, the centrifuge side line 21b is connected to a branch connector 21f for connection with the tube 50, and has a first pump tube 21g formed in the middle thereof.

  One end side of the collection bag side line 22 is connected to the discharge port 144 of the centrifuge 20. The collection bag side line 22 includes a branch connector 22b for connection to the tubes 42 and 43.

  Further, the turbidity sensor 14 and the bubble sensor 34 are attached to the collection bag side line 22 from the centrifuge 20 side. The turbidity sensor 14 and the bubble sensor 34 are collectively installed on the front side of the upper surface of the apparatus main body 3 (see FIG. 1), and are arranged between the centrifuge 20 and the branch connector 22b on the circuit. .

  The branch connector 22b is connected with a filter 22f that is air-permeable and bacteria-impermeable through a tube 41. This line can be used, for example, for detecting the internal pressure of the collection bag side line 22.

  One end of the anticoagulant injection line 23 is connected to a connection branch connector 21 c provided in the blood collection / return line 21. That is, the anticoagulant injection line 23 branches from the blood collection / return line 21 via the branch connector (branch part) 21c. The branch connector 21c is located (provided) in the vicinity of the blood collection needle 29.

  The anticoagulant injection line 23 includes a second pump tube 23a, a sterilization filter (foreign matter removal filter) 23b, a bubble removal chamber 23c, and an anticoagulant container connection needle 23d from the branch connector 21c side. And.

  A bubble sensor 31 (third detection means) is attached to the tube of the anticoagulant injection line 23. As described above, the bubble sensor 31 is installed side by side with the bubble sensor 32 in the apparatus main body 3, and is arranged between the branch connector 21c and the second pump tube 23a on the circuit.

  The anticoagulant container connection needle 23d of the anticoagulant injection line 23 is connected to a bag (container) 61 in which an anticoagulant (anticoagulant liquid) is housed (contained), whereby the anticoagulant container connection needle 23d. As will be described later, the coagulant flows through the anticoagulant injection line 23 from the anticoagulant container connection needle 23d toward the branch connector 21c, and is supplied (injected) to the blood collection needle side line 21a. Thereby, for example, the anticoagulant can be added (mixed) to the blood collected by the blood collection needle 29 via the anticoagulant injection line 23.

  In addition, although it does not specifically limit as an anticoagulant, For example, ACD-A liquid etc. can be used.

  The plasma collection bag 25 which is a blood component collection bag is a container for collecting (storing) plasma. One end of the tube 49 is connected to the plasma collection bag 25, and a connecting branch connector 22d is provided in the middle thereof. One end of the tube 50 is connected to the branch connector 22d, and the other end is connected to the branch connector 21f.

  One end of the tube 43 is connected to the branch connector 22b, and the other end is provided with a connection branch connector 22c. One end of the tube 44 is connected to the branch connector 22c, and the other end is connected to the plasma collection bag 25.

  A bubble sensor 33 is mounted in the middle of the tube 46. The bubble sensor 33 is installed on the back side of the upper surface of the apparatus body 3 (see FIG. 1).

  The plasma collection bag 25 and tubes 43 and 44 constitute a plasma collection branch line for collecting plasma.

  A platelet (platelet preparation) collection bag 26, which is a blood component collection bag, is a container for collecting (holding) plasma containing platelets after passing through a leukocyte removal filter 261 described later. In the following description, plasma containing platelets is referred to as “concentrated platelets”, and concentrated platelets collected (stored) in the platelet collection bag 26 are referred to as “platelet preparations”.

  One end of the tube 51 is connected to the platelet collection bag 26, and the bag 28 is connected to the other end.

  The air bag 27b is a container for temporarily storing (reserving) air.

  At the time of blood collection to be described later, air (sterilized air) in the blood component collection circuit 2 such as in the blood storage space 146 of the centrifuge 20 is transferred and stored in the air bag 27b. In the blood return process (blood component return process), the air stored in the air bag 27b is transferred into the blood storage space 146 of the centrifuge 20 and returned. Thereby, a predetermined blood component is returned to the blood donor.

  One end of the tube 42 is connected to the branch connector 22b, and the other end is connected to the airbag 27b.

  The intermediate bag (temporary storage bag) (first container) 27a, which is a blood component collection bag, is a container (storage part) for collecting (temporarily storing) concentrated platelets (first blood component). One end of the tube 45 is connected to the branch connector 22c, and the other end is connected to the intermediate bag 27a.

  One end of the tube 46 is connected to the intermediate bag 27a, and a connecting branch connector 22e is provided at the other end. The other end of the tube 49 is connected to the branch connector 22e.

  In addition, one end of a tube 47 is connected to the branch connector 22e for connection, and a leukocyte removal filter (cell separation filter) (filtering) that separates and removes leukocytes (predetermined cells) from the concentrated platelets is provided in the middle of the tube 47. 261) is installed.

  The other end of the tube 47 is provided with a connecting branch connector 22g, and the other end of the tube 48, one end of which is connected to the platelet collection bag 26, is connected to the branch connector 22g.

  Further, a filter main body provided with a vent filter and a filter 22h provided with a cap are installed at the port of the branch connector 22g.

  Here, the tubes 46 and 47 constitute a supply tube for supplying concentrated platelets from the intermediate bag 27a to the leukocyte removal filter 261, and the tube 48 is concentrated platelets after separating and removing leukocytes from the leukocyte removal filter 261. Is discharged (supplied to the platelet collection bag 26).

  That is, the tubes 46, 47, 48, the intermediate bag 27a, the leukocyte removal filter 261 and the platelet collection bag 26 constitute a filtration line for separating and removing leukocytes from the concentrated platelets.

  With the blood component collection device 1 assembled (when the blood component collection device 1 is used), the intermediate bag 27a, the leukocyte removal filter 261, the platelet collection bag 26, and the plasma collection bag 25 are respectively the intermediate bag 27a. The leukocyte removal filter 261 is set at a position lower than the plasma collection bag 25 (downward in the vertical direction) at a position lower than the intermediate bag 27a, and the platelet collection bag 26 is set at a position lower than the leukocyte removal filter 261 (positioned). The intermediate bag 27a and the plasma collection bag 25 are respectively positioned higher (vertically upward) than the blood storage space 146 of the rotor 142 of the centrifuge 20.

  In this case, the apparatus main body 3 includes hangers (hooks) 301, 302, and 303, which are support portions that detachably support the plasma collection bag 25, the intermediate bag 27a and the air bag 27b, and the bag 61, respectively. Is provided.

  Further, as the leukocyte removal filter 261, for example, in a casing having an inlet and an outlet at both ends, for example, a woven fabric, a nonwoven fabric, a mesh, a foam or the like made of a synthetic resin such as polypropylene, polyester, polyurethane, polyamide, etc. The thing constituted by inserting the filtration member which laminated one layer or two layers or more of a porous body can be used.

  The constituent materials of the tubes used for forming the first to anticoagulant injection lines 21 to 23, the pump tubes 21g and 23a, and the other tubes 41 to 51 and 21h are respectively polychlorinated. Vinyl is preferred.

  If these tubes are made of polyvinyl chloride, sufficient flexibility and softness can be obtained, so that they are easy to handle and are suitable for clogging with a clamp or the like.

  Further, as the constituent materials of the branch connectors 21c, 21f, 22b, 22c, 22d, 22e, and 22g described above, the same materials as those described for the tube can be used.

  In addition, as each pump tube 21g and 23a, what has the intensity | strength of the grade which is not damaged even if it presses with each liquid feeding pump (for example, roller pump etc.) 11 and 12 mentioned later, respectively is used.

  The plasma collection bag 25, the platelet collection bag 26, the intermediate bag 27a, the air bag 27b, and the bags 28 and 61 are each laminated with a resin-made flexible sheet material, and the peripheral portions thereof are fused (heat fusion, High-frequency fusion, ultrasonic fusion, etc.) or a bag formed by bonding with an adhesive is used. As described above, the air bag 27b and the intermediate bag 27a are integrally formed (integrated).

  As a material used for each bag 25, 26, 27a, 27b, 28, 61, for example, soft polyvinyl chloride is preferably used.

  In addition, as a sheet material used for the platelet collection bag 26, it is more preferable to use a material excellent in gas permeability in order to improve platelet storage stability.

  As such a sheet material, for example, polyolefin, DnDP plasticized polyvinyl chloride, or the like is used, and a sheet material of the above-described material is used without using such a material. What was thin (for example, about 0.1-0.5 mm, especially about 0.1-0.3 mm) is suitable.

  The main part of such a blood component collection circuit 2 is, for example, a cassette type. That is, the blood component collection circuit 2 partially stores each line (blood collection / return line 21, collection bag side line 22, anticoagulant injection line 23) and each predetermined tube, and partially stores them. Holding, in other words, a cassette housing 70 in which they are partially fixed is provided.

  Both ends of the first pump tube 21g and the both ends of the second pump tube 23a are fixed to the cassette housing 70. These pump tubes 21g and 23a are respectively supplied from the cassette housing 70 to the respective liquid feed pumps (for example, Projecting in a loop shape corresponding to the shape of 11, 12). For this reason, the 1st and 2nd pump tubes 21g and 23a are easy to mount on the liquid feeding pumps 11 and 12, respectively. Further, the cassette housing 70 is formed with holes (openings) into which the respective flow path opening / closing means 81 to 87 described later can be inserted.

  The centrifuge 20 provided in the blood component collection circuit 2 is generally called a centrifuge bowl, and separates blood into a plurality of blood components by centrifugal force.

  As shown in FIG. 3, the centrifugal separator 20 has a vertically extending tube body 141 with an inlet 143 formed at the upper end, and rotates around the tube body 141 and is liquid-tightly sealed with respect to the upper portion 145. And a hollow rotor 142.

  An annular blood storage space 146 is formed in the rotor 142 along the inner surface of the peripheral wall. The blood storage space 146 has a shape (tapered shape) in which the inner and outer diameters gradually decrease from the lower part toward the upper part in FIG. 3, and the lower part is formed in a substantially disc shape formed along the bottom part of the rotor 142. The upper end of the tubular body 141 communicates with the discharge port (outlet) 144. Further, in the rotor 142, the volume of the blood storage space 146 is, for example, about 100 to 350 mL, and the maximum inner diameter (maximum radius) from the rotating shaft of the rotor 142 is, for example, about 55 to 65 mm.

  Such a rotor 142 rotates under predetermined centrifugal conditions (rotation speed and rotation time) set in advance by the centrifuge drive device 10 mounted on the apparatus main body 3. Under this centrifugal condition, a blood separation pattern (for example, the number of blood components to be separated) in the rotor 142 can be set.

  In the present embodiment, as shown in FIG. 3, the centrifugal conditions are set so that the blood is separated from the inner layer into the plasma layer 131, the buffy coat layer 132, and the red blood cell layer 133 in the blood storage space 146 of the rotor 142.

  The device body 3 of the blood component collection device 1 includes a centrifuge drive device 10 for rotating the rotor 142 of the centrifuge 20 and a first liquid feed pump 11 installed in the middle of the blood collection / return line 21. And the second liquid feeding pump 12 installed in the middle of the anticoagulant injection line 23 and the blood component collection circuit 2 (blood collection / return line 21, tube 42, tube 44, tube 45, tube 47, tube 49 , A plurality of flow path opening / closing means 81, 82, 83, 84, 85, 86, 87 that can open and close the flow path of the tube 50), display means (notification means) for displaying (notifying) various information, and A display / operation unit 17 which is an operation means for performing each operation, a centrifuge drive device 10, a first liquid feed pump 11, a second liquid feed pump 12, a plurality of flow path opening / closing means 81 to 87, and a display / Operation unit 17 A control unit for controlling and a (control means) 13.

  Further, the device body 3 of the blood component collection device 1 includes a turbidity sensor 14 mounted (installed) on the collection bag side line 22, an optical sensor 15 installed in the vicinity of the centrifuge 20, and a plurality of bubbles. Sensors 31 to 36 and a weight sensor 16 for measuring the weight of plasma together with the plasma collection bag 25 are provided.

  The control unit 13 includes two pump controllers (not shown) for the first liquid feeding pump 11 and the second liquid feeding pump 12, and the control unit 13, the first liquid feeding pump 11, and the second liquid feeding pump 12. The liquid feed pump 12 is electrically connected via a pump controller.

  A drive controller (not shown) included in the centrifuge drive device 10 is electrically connected to the control unit 13.

Each of the channel opening / closing means 81 to 87 is electrically connected to the control unit 13.
Further, the turbidity sensor 14, the optical sensor 15, the weight sensor 16, the bubble sensors 31 to 36, and the display / operation unit 17 are each electrically connected to the control unit 13.

  The control unit 13 is composed of, for example, a microcomputer. The control unit 13 receives detection signals from the turbidity sensor 14, the optical sensor 15, the weight sensor 16, and the bubble sensors 31 to 36, as needed. Entered. A signal (input) from the display / operation unit 17 is also input to the control unit 13.

  The control unit 13 collects blood components according to a preset program based on the detection signals from the turbidity sensor 14, the optical sensor 15, the weight sensor 16, the bubble sensors 31 to 36 and the signal from the display / operation unit 17. The operation of each part of the apparatus 1, that is, the rotation, stop, and rotation direction (forward / reverse rotation) of each of the liquid feed pumps 11 and 12 is controlled. The operation of the separator driving device 10 and the driving of the display / operation unit 17 are controlled.

  Further, the control unit 13 has a function of measuring the liquid feeding amount of the anticoagulant based on the operation amount of the second liquid feeding pump 12. The apparatus body 3 is provided with a rotary encoder (not shown) for detecting the rotation amount of the second liquid feeding pump 12, and the control unit 13 detects the detected rotation amount of the second liquid feeding pump 12. Based on the known inner diameter of the pump tube 23a, the amount of liquid fed by the second liquid feed pump 12 can be measured (calculated). In addition, when the motor 203 of the centrifuge drive device 10 is configured by a pulse motor, the rotation amount of the second liquid feeding pump 12 may be detected based on the number of drive pulses.

  The first flow path opening / closing means 81 is provided to open and close the blood collection / return line 21 from the first pump tube 21g to the blood collection needle 29 side, that is, between the branch connector 21f and the chamber 21d.

  The second flow path opening / closing means 82 is provided to open / close the tube 50. The third flow path opening / closing means 83 is provided for opening and closing the tube 44. The fourth flow path opening / closing means 84 is provided to open and close the tube 45. The fifth flow path opening / closing means 85 is provided for opening and closing the tube 42. The sixth flow path opening / closing means 86 is provided to open and close the tube 49. The seventh flow path opening / closing means 87 is provided to open / close the tube 47.

  Each of the channel opening / closing means 81 to 87 includes an insertion part into which the blood collection / return line 21 and the tubes 50, 44, 45, 42, 49, 47 can be inserted. It has a clamp that operates with a drive source such as an electric motor or cylinder (hydraulic or pneumatic). Specifically, an electromagnetic clamp that operates with a solenoid is suitable.

  Each of these channel opening / closing means (clamps) 81 to 87 operates based on a signal from the control unit 13.

  The display / operation unit 17 includes, for example, a touch panel including a liquid crystal display panel, an EL display panel, and the like.

  In addition, a display unit (for example, a liquid crystal display panel, an EL display panel, etc.) that is a display unit (notification unit) for displaying (notifying) various information, and an operation unit (for example, an operation button) that is an operation unit for performing each operation. , An operation switch, an operation dial, etc.) may be provided separately.

  As shown in FIG. 3, the centrifuge drive device 10 includes a housing 201 that houses the centrifuge 20, a leg 202, a motor 203 that is a drive source, and a disk-shaped fixing that holds the centrifuge 20. And a table 205.

  The housing 201 is placed and fixed on the upper portion of the leg portion 202. In addition, a motor 203 is fixed to the lower surface of the housing 201 via a spacer 207 with bolts 206.

  A fixed base 205 is fitted on the tip of the rotating shaft 204 of the motor 203 so as to rotate coaxially and integrally with the rotating shaft 204, and the bottom of the rotor 142 is fitted on the upper portion of the fixed base 205. A concave portion is formed.

  The upper portion 145 of the centrifuge 20 is fixed to the housing 201 by a fixing member (not shown).

  In such a centrifuge drive device 10, when the motor 203 is driven, the fixed base 205 and the rotor 142 fixed thereto rotate at, for example, about 3000 to 6000 rpm.

  The optical sensor 15 is installed on the side of the housing 201 (left side in FIG. 3).

  The optical sensor 15 is configured to project light toward the blood storage space 146 and to receive the reflected light.

  The optical sensor 15 irradiates (projects) light (for example, laser light) from the light projecting unit 151, and the reflected light reflected by the reflecting surface 147 of the rotor 142 is received by the light receiving unit 152. The light receiving unit 152 converts the received light quantity into an electrical signal.

  Here, the optical sensor 15 has a reflecting surface on one side and a reflecting plate 153 that changes the optical path, and the light irradiated from the light projecting unit 151 passes through the reflecting plate 153 and is reflected on the reflecting surface 147. The light that is irradiated to the light and reflected by the reflecting surface 147 is received by the light receiving unit 152 via the reflecting plate 153.

  At this time, the projection light and the reflected light are transmitted through the blood component in the blood storage space 146, but the position of the blood component interface (the interface B between the plasma layer 131 and the buffy coat layer 132 in this embodiment). Accordingly, since the abundance ratio of each blood component at a position where the light projection light and the reflected light are transmitted is different, the transmittance thereof is changed. As a result, the amount of light received by the light receiving unit 152 varies (changes), and this variation can be detected as a change in the output voltage from the light receiving unit 152.

  That is, the optical sensor 15 can detect the position of the blood component interface based on the change in the amount of light received by the light receiving unit 152.

  The interface of the blood component detected by the optical sensor 15 is not limited to the interface B, and may be the interface between the buffy coat layer 132 and the red blood cell layer 133, for example.

  Here, each of the layers 131 to 133 in the blood storage space 146 has a different color depending on the blood component, and in particular, the red blood cell layer 133 is red with the color of the red blood cells. For this reason, from the viewpoint of improving the accuracy of the optical sensor 15, there is a range suitable for the wavelength of the projection light, and the wavelength range is not particularly limited, but is preferably about 600 to 900 nm, for example. 750 to 800 nm is more preferable.

  The turbidity sensor 14 is for detecting the turbidity (platelet concentration) of the fluid flowing in the collection bag side line 22 and outputs a voltage value corresponding to the turbidity. Specifically, the turbidity sensor 14 outputs a low voltage value when the turbidity is high and a high voltage value when the turbidity is low.

  The turbidity sensor 14 can detect, for example, the concentration of platelets in plasma flowing through the collection bag side line 22, changes in the concentration of platelets in plasma, contamination of red blood cells into the plasma, and the like. As the turbidity sensor 14, for example, an ultrasonic sensor, an optical sensor, an infrared sensor, or the like can be used.

  In addition, the bubble sensor 34 can detect, for example, the replacement of fluid flowing in the collection bag side line 22 from air to plasma.

  As the first liquid delivery pump 11 to which the first pump tube 21g is attached and the second liquid delivery pump 12 to which the second pump tube 23a is attached, for example, non-blood such as a roller pump, respectively. A contact type pump is preferably used.

  Further, as the first liquid feeding pump (blood pump) 11, a pump capable of feeding blood in any direction is used. Specifically, a roller pump capable of forward rotation and reverse rotation is used.

  When blood is collected from a blood donor using such a blood component collection device 1, a new blood component collection circuit 2 is attached to the device body 3. At this time, each of the bubble sensors 31 to 36 includes It is necessary to correctly attach a tube of a predetermined line as described above.

  However, the tube of the anticoagulant injection line 23 is erroneously attached to the bubble sensors 35 and 36 to which the blood collection / return line 21 (blood collection needle side line 21a) should be attached due to the error of the operator (nurse). There is a case.

  FIG. 4 is a developed view of the blood component collection circuit when the above-described erroneous mounting (hereinafter, also simply referred to as “tube erroneous mounting”) occurs. As shown in the figure, when the tube is erroneously attached, the anticoagulant injection line 23 is continuously attached with the bubble sensors 35 and 36 on the branch connector 21c side of the bubble sensor 31.

  The bubble sensors 35 and 36 play an important role of monitoring the presence or absence of bubbles in the blood collection needle side line 21a so as not to inject bubbles into the body of the blood donor when returning blood. If the blood component collection operation as described later is performed as it is, the role cannot be fulfilled.

  Therefore, the blood component collection device 1 of the present invention detects erroneous tube attachment during a priming operation before starting blood collection from a blood donor (before puncturing the blood collection needle 29 into the blood vessel of the blood donor). When wearing, the operator is informed accordingly.

  FIG. 5 is a flowchart of the control unit 13 when detecting erroneous tube attachment during the priming operation. Hereinafter, based on the same figure, the control at the time of the control part 13 detecting erroneous tube attachment is demonstrated.

  In the priming operation, the anticoagulant in the bag 61 is filled into the entire anticoagulant injection line 23 and the blood collection needle 29 of the blood collection / return line 21 to the chamber 21d. When the operator presses the priming start button displayed on the display / operation unit 17, the priming operation is started, and the second liquid feeding pump 12 starts rotating (step S001). Thus, the anticoagulant in the bag 61 is injected into the anticoagulant injection line 23 from the anticoagulant container connection needle 23d side, and the gas-liquid separation surface at the tip of the anticoagulant is connected to the anticoagulant container connection. It proceeds from the needle 23d side toward the branch connector 21c side.

  When the controller 13 starts to rotate the second liquid pump 12, based on the operation amount of the second liquid pump 12, the anticoagulant liquid is supplied from the start of the rotation of the second liquid pump 12. The measurement of the amount (integrated liquid feeding amount) is started (step S002).

  Thereafter, the controller 13 monitors whether the bubble sensor 31 detects liquid (step S003). When the anticoagulant in the anticoagulant injection line 23 reaches the position of the bubble sensor 31 and the bubble sensor 31 detects liquid, the control unit 13 determines the bubble based on the operation amount of the second liquid feed pump 12. Measurement of the anticoagulant feeding amount (integrated feeding amount) after passing through the sensor 31 is started (step S004).

  Then, the control unit 13 determines whether or not the liquid feeding amount after passing through the bubble sensor 31 has reached a first predetermined amount (in this embodiment, 10 mL) (step S005). If the amount of liquid delivered after passing through the bubble sensor 31 has not reached 10 mL, it is determined whether or not the bubble sensor 35 or 36 has detected a liquid (step S006), and both the bubble sensors 35 and 36 are liquid. Is not detected, the process returns to step S005, and step S005 and subsequent steps are executed again.

  Here, when the blood collection / return line 21 (blood collection needle side line 21a) is correctly attached to the bubble sensors 35, 36, the anticoagulant in the anticoagulant injection line 23 as shown in FIG. After reaching the bubble sensor 31, the anticoagulant injection line 23 is filled over the entire length, and further passes through the branch connector 21 c and proceeds on the blood collection needle side line 21 a before reaching the bubble sensors 35 and 36. In this case, since the circuit length from the bubble sensor 31 to the bubble sensors 35 and 36 is as long as about 1 to 2 m, the second time until the anticoagulant reaches the bubble sensor 35 after reaching the bubble sensor 31 is reached. The liquid feeding amount of the liquid feeding pump 12 is always larger than 10 mL.

  On the other hand, when the anticoagulant injection line 23 is mistakenly attached to the bubble sensors 35 and 36, the bubble sensors 35 and 36 are provided immediately after the bubble sensor 31 as shown in FIG. Since the circuit length from 31 to the bubble sensors 35 and 36 is as short as several tens of centimeters, the second liquid-feeding pump 12 from when the anticoagulant reaches the bubble sensor 31 until it reaches the bubble sensors 35 and 36 is short. The amount of liquid delivered is 10 mL or less. Thus, the first predetermined amount is set by the distance (positional relationship) between the bubble sensor 31 and the bubble sensors 35 and 36.

  Therefore, when the bubble sensor 35 or 36 detects a liquid in step S006, that is, when the anticoagulant reaches the bubble sensors 35 and 36 until the liquid supply amount after passing through the bubble sensor 31 reaches 10 mL. The control unit 13 determines that the tube is incorrectly installed, and displays a text, a symbol, a mark, or the like warning that the tube is incorrectly installed on the display / operation unit 17 in order to notify the operator of the fact (Step). S007). Or you may alert | report with an alarm sound, an audio | voice, etc.

  When it is determined that the tube is erroneously attached, the control unit 13 further stops the rotation of the second liquid feeding pump 12 and stops the priming operation (step S008). In the meantime, the operator can remove the anticoagulant injection line 23 from the bubble sensors 35 and 36 and correctly attach the blood collection / return line 21 (blood collection needle side line 21a). Thereafter, when the operator presses the priming restart button displayed on the display / operation unit 17, the priming operation is restarted.

  On the other hand, when the bubble sensor 35 or 36 does not detect the liquid until the liquid supply amount after passing through the bubble sensor 31 reaches 10 mL, the control unit 13 operates according to the operation of the second liquid supply pump 12. The liquid supply of the anticoagulant is continued and the priming operation is continued (step S009).

  Then, the control unit 13 determines whether or not the liquid supply amount from the start of rotation of the second liquid supply pump 12 has reached a second predetermined amount (35 mL in the present embodiment) (step S010). ). When the blood collection / return line 21 (blood collection needle side line 21a) is correctly attached to the bubble sensors 35, 36, the second predetermined amount is that the anticoagulant reliably reaches the bubble sensors 35, 36. The amount is determined to be likely to be, and is set in advance based on the lengths of the anticoagulant injection line 23 and the blood collection needle side line 21a.

  In step S010, if the liquid supply amount from the start of rotation of the second liquid supply pump 12 has not reached 35 mL, it is determined whether the bubble sensor 35 or 36 has detected a liquid (step S011). ) If both the bubble sensors 35 and 36 have not detected liquid, the process returns to step S010, and step S010 and subsequent steps are executed again.

  In step S011, when the bubble sensor 35 or 36 detects a liquid, that is, until the amount of liquid fed from the start of rotation of the second liquid feed pump 12 reaches 35 mL, the anticoagulant is added to the bubble sensor 35, When reaching 36, the control unit 13 determines that the blood collection / return line 21 (blood collection needle side line 21a) is correctly attached to the bubble sensors 35, 36. After that, when the liquid supply amount reaches a predetermined amount (an amount that fills the chamber 21d of the blood collection / return line 21 with the anticoagulant), the control unit 13 performs the second liquid supply pump 12. Is stopped (step S013). As a result, the priming operation ends normally.

  In addition, when the bubble sensor 35 or 36 does not detect the liquid until the liquid supply amount from the start of rotation of the second liquid supply pump 12 reaches 35 mL, the control unit 13 causes the bubble sensor 35 to detect the liquid. 36, the blood collection / return line 21 is not attached (no tube of any line is attached to the bubble sensor 35, 36), and the operator is warned that the tube is not attached to inform the operator to that effect. A text, a symbol, a mark, or the like is displayed on the display / operation unit 17 (step S012). Or you may alert | report with an alarm sound, an audio | voice, etc.

  As described above, in the blood component collection device 1 of the present invention, the tube misinstallation can be detected during the priming operation, which is the stage before the blood collection from the blood donor, and the tube misinstallation occurs. Even if it exists, it can be re-mounted correctly by the operator in advance, so it is extremely safe.

  Furthermore, in this embodiment, since it is possible to detect forgetting to attach the blood collection / return line 21 to the bubble sensors 35, 36, the operator forgets to attach any line of tubes to the bubble sensors 35, 36. Even if it is, this can be detected and notified to the operator, which is even more secure.

  In this embodiment, determination is made based on the amount of liquid fed after passing through the bubble sensor 31 in step S005, and based on the amount of liquid fed from the start of rotation of the second liquid feed pump 12 in step S010. Although determination is being made, both steps S005 and S010 may be determined based on the amount of liquid fed after passing through the bubble sensor 31, or both steps S005 and S010 may be performed by rotating the second liquid feed pump 12. It is good also as judging based on the liquid feeding amount from the start.

  In addition, when the bubble sensor 35 or 36 does not detect the liquid until the liquid supply amount reaches the first predetermined amount (10 mL), the control unit 13 correctly sets the blood collection / return line 21 to the bubble sensor. It may be determined that it is attached to 35 and 36, and the determination in step S010 may not be performed.

  When the priming operation as described above is completed, the blood collection needle 29 is punctured into the blood vessel of the blood donor, and the platelet collection operation (blood component collection operation) is started. This platelet collection operation will be described with reference to the flowcharts shown in FIGS.

  Under the control of the control unit 13, the blood component collection device 1 includes a first plasma collection process, a constant-speed plasma circulation process, a second plasma collection process, an accelerated plasma circulation process, and a third plasma collection process. The platelet collection operation (blood component collection operation) having a platelet collection step and a blood return step (blood component return step) is performed. This platelet collection operation is performed at least once.

  In the present embodiment, the platelet collection operation is repeated a plurality of times (1st cycle to nth cycle, n is an integer of 2 or more).

  In parallel with the final-cycle platelet collection operation, the blood component collection device 1 controls the control unit 13 to control the concentrated platelets temporarily collected (stored) in the intermediate bag 27a. 261 to perform a filtering operation (filtering step) for separating and removing white blood cells in the concentrated platelets.

  The timing for starting this filtration operation is not particularly limited, but from the viewpoint of shortening the donor's restraint time, this filtration operation is performed at the same time as the platelet collection operation in the final cycle (particularly, the process at an early stage of the platelet collection operation). In). In the blood component collection device 1 of the present embodiment, the filtration operation is configured to start almost simultaneously with the start of the second plasma collection step in the platelet collection operation in the final cycle. The description of the operation (starting filtration process) step is omitted.

[1] Platelet collection operation in the first cycle (see FIGS. 6 and 7)
[11] First, the blood component collection device 1 performs a first plasma collection step. In the first plasma collection step, blood is introduced into the blood storage space 146 of the rotor 142, and the plasma (PPP) separated by centrifuging the blood is collected in the plasma collection bag 25.

  In the first plasma collection step, first, the control unit 13 collects plasma (second blood component) (step S101 in FIG. 6).

  Specifically, under the control of the control unit 13, the first liquid supply / closing means 81 and the fifth flow path opening / closing means 85 are opened, and the other liquid flow opening / closing means are closed, and the first liquid supply The pump 11 is operated (forward rotation) at a predetermined rotation speed (preferably about 250 mL / min or less, more preferably about 40 to 150 mL / min, for example, 60 mL / min), and blood collection is started from the blood donor.

  Simultaneously with this blood collection, the control of the control unit 13 activates the second liquid feeding pump 12 to supply an anticoagulant such as an ACD-A solution via the anticoagulant injection line 23. The anticoagulant is mixed into the blood sample.

  At this time, the rotation speed of the second liquid feeding pump 12 is adjusted by the control unit 13 so that the anticoagulant is mixed with the blood sample at a predetermined ratio (preferably about 1/20 to 1/6, for example, 1/10). To be controlled.

  As a result, blood (blood added with an anticoagulant) is transferred through the blood collection / return line 21 and introduced into the blood storage space 146 of the rotor 142 from the inlet 143 of the centrifuge 20 via the tube 141. .

  At this time, the air (sterilized air) in the centrifuge 20 is sent into the air bag 27 b through the collection bag side line 22 and the tube 42.

  Simultaneously with or before or after the blood collection, the control unit 13 operates the centrifuge drive device 10 to control the rotor 142 to rotate at a predetermined rotation speed.

  By the rotation of the rotor 142, the blood introduced into the blood storage space 146 is separated from the inside into three layers: a plasma layer (PPP layer) 131, a buffy coat layer (BC layer) 132, and a red blood cell layer (CRC layer) 133. The

  The rotational speed of the rotor 142 is preferably about 3000 to 6000 rpm, more preferably about 4200 to 5800 rpm. In the following steps, the control unit 13 does not change the rotational speed of the rotor 142 unless otherwise specified.

  Further, when the blood collection and the supply of the anticoagulant are continued and blood (about 270 mL) exceeding the capacity of the blood storage space 146 is introduced into the blood storage space 146, the blood storage space 146 is filled with blood and centrifuged. Plasma (PPP) flows out from the outlet 144 of the vessel 20.

  At this time, the bubble sensor 34 installed in the collection bag side line 22 detects that the fluid flowing in the collection bag side line 22 has changed from air to plasma, and the control unit 13 detects the bubble sensor 34. Based on the signal, control is performed so that the fifth flow path opening / closing means 85 is closed and the third flow path opening / closing means 83 is opened.

  As a result, plasma is introduced and collected into the plasma collection bag (third container) 25 via the collection bag side line 22 and the tubes 43 and 44.

  The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 13.

  Next, the control unit 13 determines whether or not a predetermined amount of plasma has been collected in the plasma collection bag 25 based on information (weight signal) from the weight sensor 16 (step S102 in FIG. 6).

  The amount of plasma collected (predetermined amount) is preferably about 10 to 150 g, more preferably about 20 to 40 g.

  In step S102, when a predetermined amount of plasma is not collected in the plasma collection bag 25, the control unit 13 returns to step S101 and repeats step S101 and subsequent steps again.

  In step S102, when a predetermined amount of plasma is collected in the plasma collection bag 25, the control unit 13 ends this step [11] (first plasma collection step) and performs constant-speed plasma. Move to circulation process.

[12] Next, the blood component collection device 1 performs a constant-speed plasma circulation step. In the constant-speed plasma circulation step, the plasma in the plasma collection bag 25 is circulated at a constant speed through the blood storage space 146.

  In the constant-speed plasma circulation step, first, the control unit 13 circulates plasma (step S103 in FIG. 6).

  Specifically, under the control of the control unit 13, the first flow path opening / closing means 81 is closed, the second flow path opening / closing means 82 is opened, the second liquid feed pump 12 is stopped, and the first flow path opening / closing means 82 is stopped. The liquid feed pump 11 is operated (forward rotation) at a predetermined rotational speed (preferably about 60 to 250 mL / min, for example, 200 mL / min).

  Thereby, the blood collection is temporarily interrupted, and the plasma in the plasma collection bag 25 is introduced into the blood storage space 146 through the tubes 49 and 50 and the blood collection / return line 21 at a constant speed. The plasma flowing out from 144 is collected into the plasma collection bag 25 through the collection bag side line 22 and the tubes 43 and 44. That is, the plasma in the plasma collection bag 25 is circulated in the blood storage space 146 at a constant speed.

  Next, the control unit 13 determines whether or not a predetermined time (preferably about 10 to 90 seconds, for example, 30 seconds) has elapsed since the start of constant-speed plasma circulation (step S104 in FIG. 6).

  In step S104, when the predetermined time has not elapsed since the start of constant-speed plasma circulation, the control unit 13 returns to step S103, and repeats step S103 and subsequent steps again.

  In step S104, when a predetermined time has elapsed since the start of constant-speed plasma circulation, the control unit 13 ends this step [12] (constant-speed plasma circulation step), and the second plasma Move to the collection process.

[13] Next, the blood component collection device 1 performs a second plasma collection step. In the second plasma collection step, blood is introduced into the blood storage space 146 of the rotor 142, and the plasma separated by centrifuging the blood is collected in the plasma collection bag 25.

  In the second plasma collecting step, the step [11] except that the position of the interface B between the plasma layer 131 and the buffy coat layer 132 is detected instead of measuring the amount of collected plasma by the weight sensor 16. A step similar to the (first plasma collection step) is performed.

  In the second plasma collection step, first, the control unit 13 collects plasma (step S105 in FIG. 6).

  At this time, the control unit 13 controls the second flow path opening / closing means 82 to be closed and the first flow path opening / closing means 81 to be opened.

  Thereby, as the amount of red blood cells in the blood storage space 146 increases, that is, as the layer thickness of the red blood cell layer 133 increases, the interface B gradually moves in the direction of the rotation axis of the rotor 142.

  Next, the control unit 13 determines whether or not the interface B has reached a predetermined level (first position) based on the detection signal (interface position detection information) from the optical sensor 15 (step S106 in FIG. 6). ).

  The first position of the interface B is preferably the position at which the detection signal from the first optical sensor 15 (output voltage from the light receiving unit 152) is about 1 to 2V. .

  In step S106, when the interface B has not reached the first position, the control unit 13 returns to step S105 and repeats step S105 and subsequent steps again.

  In Step S106, when interface B reaches the 1st position, control part 13 ends this process [13] (2nd plasma collection process), and shifts to an acceleration plasma circulation process. .

[14] Next, the blood component collection device 1 performs an accelerated plasma circulation step. In the accelerated plasma circulation step, the plasma in the plasma collection bag 25 is circulated while being accelerated into the blood storage space 146.

  In the accelerated plasma circulation process, first, the control unit 13 circulates plasma (step S107 in FIG. 6).

  Specifically, under the control of the control unit 13, the first flow path opening / closing means 81 is closed, the second flow path opening / closing means 82 is opened, the second liquid feed pump 12 is stopped, and It operates (forward rotation) so that the rotational speed of the first liquid feeding pump 11 increases (increases) at a constant acceleration.

  As a result, the blood collection is temporarily interrupted, and the plasma in the plasma collection bag 25 is introduced into the blood storage space 146 through the tubes 49 and 50 and the blood collection / return line 21 while being accelerated. The plasma flowing out from 144 is collected into the plasma collection bag 25 through the collection bag side line 22 and the tubes 43 and 44. That is, the plasma in the plasma collection bag 25 is circulated while being accelerated into the blood storage space 146.

  At this time, the control unit 13 increases (increases) the rotation speed of the first liquid feeding pump 11 at a constant acceleration from a speed (initial speed: for example, 60 mL / min) slower than the constant-speed plasma circulation. To control.

  The acceleration condition (acceleration) is preferably about 1 to 10 mL / min / sec, more preferably about 3 to 6 mL / min / sec. Further, the acceleration may not be constant, and may change stepwise or continuously within the above range, for example.

  Next, the control unit 13 determines whether or not the circulating speed of plasma into the blood storage space 146 has reached a predetermined speed, that is, the rotational speed of the first liquid feeding pump 11 is a predetermined speed (preferably 130 to 250 mL / min). It is determined whether or not a certain level (eg, 155 mL / min) has been reached (step S108 in FIG. 6).

  This step S108 is continued until the circulating speed of plasma into the blood storage space 146 reaches a predetermined speed.

  In step S108, when the circulation speed of plasma into the blood storage space 146 reaches a predetermined speed, the control unit 13 ends this process [14] (accelerated plasma circulation process), and collects the third plasma. Move to the process.

[15] Next, the blood component collection device 1 performs a third plasma collection step. In the third plasma collection step, blood is introduced into the blood storage space 146 of the rotor 142, and the plasma separated by centrifuging the blood is collected in the plasma collection bag 25.

  In the third plasma collection step, first, the control unit 13 collects plasma (step S109 in FIG. 6).

  Next, the control unit 13 determines whether or not a predetermined amount of plasma has been collected in the plasma collection bag 25 based on the amount and the number of rotations per rotation of the first solution pump 11 (FIG. 6). Step S110).

  The amount of plasma collected (predetermined amount) is preferably about 2 to 30 g, more preferably about 5 to 15 g.

  In step S110, when a predetermined amount of plasma is collected in the plasma collection bag 25, the control unit 13 ends this step [15] (third plasma collection step), and the platelet collection step. (Transition to 1 in FIG. 7).

[16] Next, blood component collection device 1 performs a platelet collection step. In the platelet collection step, the plasma in the plasma collection bag 25 is circulated in the blood storage space 146 while being accelerated at the first acceleration, and then changed to a second acceleration larger than the first acceleration. The blood is circulated while being accelerated at an acceleration of 2, the platelets are discharged from the blood storage space 146, and the concentrated platelets are collected (stored) in the intermediate bag 27a.

  In the platelet collection step, first, the control unit 13 performs plasma circulation with the first acceleration (step S111 in FIG. 7).

  Specifically, under the control of the control unit 13, the first flow path opening / closing means 81 is closed, the second flow path opening / closing means 82 is opened, the second liquid feed pump 12 is stopped, and It operates (forward rotation) so as to increase (increase) the rotation speed of the first liquid feeding pump 11 at the first acceleration.

  As a result, the blood collection is interrupted, and the plasma in the plasma collection bag 25 is introduced into the blood storage space 146 through the tubes 49, 50 and the blood collection / return line 21 while being accelerated at the first acceleration, and then centrifuged. Plasma flowing out from the outlet 144 of the vessel 20 is collected into the plasma collection bag 25 via the collection bag side line 22 and the tubes 43 and 44. That is, the plasma in the plasma collection bag 25 is circulated in the blood storage space 146 while being accelerated at the first acceleration.

  At this time, if plasma is circulated in the blood storage space 146 while accelerating at the first acceleration, the red blood cell layer 133 is diffused (increase in layer thickness), and the interface B gradually moves in the direction of the rotation axis of the rotor 142. To do.

  The first acceleration is preferably about 0.5 to 10 mL / min / sec, more preferably about 1.5 to 2.5 mL / min / sec. The first acceleration may not be constant, and may change stepwise or continuously within the above range, for example.

  The initial speed of the first liquid delivery pump 11 in the plasma circulation by the first acceleration is preferably about 40 to 150 mL / min, more preferably about 50 to 80 mL / min.

  Next, the control unit 13 continues step S111 until the circulation speed of plasma into the blood storage space 146 reaches a predetermined speed (step S112 in FIG. 7).

  The predetermined speed, that is, the rotational speed of the first liquid delivery pump 11 when the plasma circulation by the first acceleration is completed is preferably about 100 to 180 mL / min, more preferably 140 to 160 mL / min. About min.

  In step S112, when the circulation speed of plasma into the blood storage space 146 reaches a predetermined speed, the control unit 13 performs plasma circulation by the second acceleration (step S113 in FIG. 7).

  Specifically, under the control of the control unit 13, the acceleration of the first liquid feed pump 11 is changed from the first acceleration to the second acceleration, and the rotation speed of the first liquid feed pump 11 is changed to the second speed. It operates (forward rotation) to increase (increase) at an acceleration of. Thereby, the plasma in the plasma collection bag 25 is circulated in the blood storage space 146 while being accelerated by the second acceleration.

  At this time, if plasma is circulated in the blood storage space 146 while accelerating at the second acceleration, the red blood cell layer 133 is diffused (increase in layer thickness), and the interface B gradually moves in the direction of the rotation axis of the rotor 142. At the same time, the platelet (PC) in the buffy coat layer 132 rises (swells) against the centrifugal force and moves toward the discharge port 144 of the rotor 142.

  The second acceleration is set to be greater than the first acceleration, and is preferably about 3 to 20 mL / min / sec, more preferably about 5 to 10 mL / min / sec. Note that the second acceleration may not be constant, and may change stepwise or continuously within the above range, for example.

  Next, the control unit 13 determines whether or not the circulating speed of plasma into the blood storage space 146 has reached a predetermined speed, that is, the rotational speed of the first liquid feeding pump 11 is a predetermined speed (preferably 120 to 300 mL / min). It is determined whether or not a certain level (for example, 200 mL / min) has been reached (step S114 in FIG. 7).

  In step S114, when the circulation speed of plasma into the blood storage space 146 has not reached the predetermined speed, the control unit 13 returns to step S113 and repeats step S113 and subsequent steps again.

  In step S114, when the circulation speed of plasma into the blood storage space 146 reaches a predetermined speed, the control unit 13 continues the plasma circulation (step S115 in FIG. 7).

  Specifically, the control unit 13 controls (maintains) the rotational speed of the first liquid feed pump 11 at the predetermined speed in step S114. Thereby, the circulation rate of plasma into the blood storage space 146 is preferably about 120 to 300 mL / min, for example, 200 mL / min.

  Next, the control unit 13 determines whether or not a predetermined time (preferably about 5 to 15 seconds, for example, 10 seconds) has elapsed since the start of Step S115 (Step S116 in FIG. 7).

  In step S116, when the predetermined time has not elapsed since the start of step S115, the control unit 13 then determines that the output voltage (PC concentration voltage) from the turbidity sensor 14 is a predetermined value (preferably 2. It is determined whether or not the voltage has decreased to about 5 to 3.5 V, for example, 3.0 V (step S117 in FIG. 7).

  In step S117, if the output voltage from the turbidity sensor 14 has not decreased below the predetermined value, the control unit 13 returns to step S115 and repeats step S115 and subsequent steps again.

  While repeating steps S115 to S117, in step S116, when a predetermined time has elapsed since the start of step S115, the control unit 13 ends this step [16] (platelet collection step). Then, the process proceeds to step S122 described later.

  In step S117, when the output voltage from the turbidity sensor 14 falls below a predetermined value, that is, as the platelets flow out from the discharge port 144 of the rotor 142, it flows in the collection bag side line 22. When the platelet concentration in plasma reaches a predetermined value or more, the control unit 13 collects platelets (PC) (step S118 in FIG. 7).

  Specifically, the control unit 13 controls the third flow path opening / closing means 83 to be closed and the fourth flow path opening / closing means 84 to be opened based on the detection signal of the turbidity sensor 14.

  Thereby, concentrated platelets are introduced into the intermediate bag 27a via the collection bag side line 22 and the tubes 43 and 45, and collected (stored). At this time, since the seventh flow path opening / closing means 87 is closed, the concentrated platelets do not flow out from the intermediate bag 27a.

  Further, the control unit 13 calculates the platelet concentration (cumulative PC concentration) in the intermediate bag 27a based on the output voltage (detection signal) from the turbidity sensor 14. The platelet concentration continues to rise after the start of PC collection, and once it reaches the maximum concentration, it begins to fall.

  Next, the control unit 13 determines whether or not a predetermined time (preferably about 10 to 25 seconds, for example, 15 seconds) has elapsed since the start of PC collection (step S119 in FIG. 7).

  If the predetermined time has not elapsed since the start of the PC sampling in step S119, the control unit 13 then determines whether the output voltage (PC concentration voltage) of the turbidity sensor 14 has reached a predetermined value or less. Is determined (step S120 in FIG. 7).

  The predetermined value of the output voltage of the turbidity sensor 14 is a value in the vicinity of the time when red blood cells are mixed in the plasma flowing in the collection bag side line 22, and is preferably about 0.5 V or less.

  In step S120, when the output voltage of the turbidity sensor 14 has not reached the predetermined value or less, the control unit 13 then determines whether or not the concentrated platelets in the intermediate bag 27a have reached a predetermined amount. (Step S121 in FIG. 7).

  The collection amount (predetermined amount) of the concentrated platelets is preferably about 20 to 100 mL, more preferably about 40 to 80 mL.

  In step S121, when the concentrated platelets in the intermediate bag 27a do not reach the predetermined amount, the control unit 13 returns to step S118 and repeats step S118 and subsequent steps again.

  While repeating steps S118 to S121, when a predetermined time has elapsed since the start of PC sampling in step S119, or when the output voltage of the turbidity sensor 14 has reached a predetermined value or less in step S120 The control unit 13 ends this step [16] (platelet collection step), and proceeds to step S122 described later.

  In step S121, when the concentrated platelets in the intermediate bag 27a reach a predetermined amount, the control unit 13 opens the fifth channel opening / closing means 85 and all the other channel opening / closing means 81. ˜84, 86, 87 are closed, the first liquid pump 11 is stopped, and this step [16] (platelet collecting step) is completed.

[17] Next, the blood component collection device 1 performs a step of stopping the centrifuge 20.
In this step, first, the control unit 13 decelerates the centrifuge 20 (step S122 in FIG. 7).

Specifically, under the control of the control unit 13, the rotational speed of the centrifuge drive device 10 is decreased and the rotor 142 is decelerated.
Further, the control unit 13 stops the centrifuge 20 (step S123 in FIG. 7).

  Specifically, under the control of the control unit 13, the rotation of the centrifuge drive device 10 is stopped and the rotor 142 is stopped.

[18] Next, the blood component collection device 1 performs a blood return step. In the blood return process, blood components (remaining blood components) in the blood storage space 146 of the rotor 142 are returned.

In the blood return process, the controller 13 performs blood return (step S124 in FIG. 7).
Specifically, under the control of the control unit 13, the first flow path opening / closing means 81 and the fifth flow path opening / closing means 85 are opened, and the first liquid feed pump 11 is rotated at a predetermined rotational speed (preferably 20). Operates (reverses) at about 120 mL / min, for example, 90 mL / min.

  As a result, blood components (mainly red blood cells and white blood cells) remaining in the blood storage space 146 of the rotor 142 are discharged from the inlet 143 of the centrifuge 20 and passed through the blood collection / return line 21 (blood collection needle 29). Returned to the blood donor.

Then, after the air discharged from the centrifugal separator 20 is detected by the bubble sensor 32 and the first liquid feeding pump 11 is rotated a predetermined number of times, the first flow path opening / closing means 81 and the fifth flow path are While closing the opening / closing means 85, the first liquid pump 11 is stopped, and this step [18] (blood returning step) is completed.
Thereby, the platelet collection operation in the first cycle is completed.

[2] Platelet collection operation in the second cycle that is not the final cycle (see FIGS. 6 and 7)
Subsequently, the platelet collection operation of the second cycle is performed.

  In the platelet collecting operation in the second cycle, the same steps as the platelet collecting operation in the first cycle are performed as follows.

[21] to [28] Steps similar to the steps [11] to [18] are performed, respectively.
Thereby, the platelet collection operation in the second cycle is completed.
The same applies to the platelet collection operation after the third cycle which is not the final cycle.

[3] Platelet collection operation in the final cycle (see FIGS. 6 and 7)
Subsequently, the platelet collection operation for the final cycle is performed.

  In the platelet collection operation in the final cycle, the same process as the platelet collection operation in the first cycle is performed except that a filtration step for separating and removing white blood cells from the concentrated platelets is performed.

[31] to [37] Steps similar to the steps [11] to [17] are performed except that the filtration step is performed. The filtration process will be described in detail later.

[38] The air discharged from the centrifugal separator 20 is detected by the bubble sensor 35 or 36 to close the first flow path opening / closing means 81 and the fifth flow path opening / closing means 85, and the first liquid feed Except for stopping the pump 11 and ending this step [38] (blood return step), the same step as the step [18] is performed.
Thereby, the platelet collection operation in the final cycle is completed.

Next, the filtration process will be described.
In the present embodiment, almost simultaneously with the second plasma collection step, the control unit 13 supplies the concentrated platelets temporarily collected (stored) in the intermediate bag 27a to the leukocyte removal filter 261 to concentrate the platelets. Filtration of platelets, that is, separation and removal of white blood cells in concentrated platelets.

  Specifically, before step S105, the seventh flow path opening / closing means 87 is opened under the control of the control unit 13 to start the filtration process (not shown). Needless to say, the timing of starting the filtration step is not limited to this.

  Thereby, the concentrated platelets in the intermediate bag 27a are transferred into the platelet collection bag 26 through the tubes 46 and 47, the leukocyte removal filter 261, and the tube 48 by a drop (self-weight). At this time, most of the concentrated platelets pass through the filtration member of the leukocyte removal filter 261, but the leukocytes are captured by the filtration member. For this reason, the removal rate of leukocytes in the platelet preparation can be made extremely high.

  The transfer of the concentrated platelets from the intermediate bag 27a to the platelet collection bag 26 may be performed using a pump.

  Further, the seventh flow path opening / closing means 87 may be a clamp or the like that can manually open and close the middle of the flow path of the tube 47 instead of the one operated by the control of the control unit 13.

  The platelet collection operation is not limited to being performed a plurality of times, and may be performed only once, for example.

  The configuration of the blood component collection circuit 2 can also be set as appropriate, and is not limited to the illustrated configuration.

  As mentioned above, although the blood component plate collection apparatus of this invention was demonstrated based on embodiment of illustration, this invention is not limited to this, The structure of each part is the thing of the arbitrary structures which have the same function Can be substituted. Moreover, other arbitrary structures and processes may be added to the present invention.

  For example, the blood component collection device of the present invention obtains only a platelet product (the blood component to be collected is only concentrated platelets (platelets)), and obtains only the plasma product (the blood component to be collected is a blood component to be collected). The present invention can also be applied to a blood component plate collecting apparatus or the like (only plasma).

  In addition, the blood component collection device of the present invention is not limited to the case where it is applied to obtain a platelet preparation or a plasma preparation (the blood component to be collected is not limited to platelets or plasma). In addition, the present invention may be applied to the production of erythrocyte preparations (the blood component to be collected may be leukocytes or erythrocytes).

  In the present invention, the cells separated and removed by the cell separation filter (filter) are not limited to leukocytes.

  In the present invention, the optical sensor is not limited to the illustrated one, and may be a line sensor, for example.

  Moreover, the method of the blood component collection device of the present invention is not limited to the intermittent type, and may be, for example, a continuous type.

It is a perspective view showing an embodiment of a blood component collection device of the present invention. It is a figure which expand | deploys and shows the blood component collection circuit in the blood component collection apparatus shown in FIG. It is a partially broken sectional view of the state where the centrifuge was installed in the centrifuge drive device with which the blood component collection device shown in FIG. 1 is provided. It is a figure which expand | deploys and shows the blood component collection circuit at the time of the incorrect attachment of the tube to a bubble sensor. It is a flowchart of the control part at the time of detecting tube incorrect attachment at the time of priming operation | movement. It is a flowchart for demonstrating the effect | action of the blood component collection device shown in FIG. 1 and FIG. It is a flowchart for demonstrating the effect | action of the blood component collection device shown in FIG. 1 and FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Blood component collection apparatus 2 Blood component collection circuit 3 Apparatus main body 10 Centrifugal drive device 11 1st liquid feeding pump 12 2nd liquid feeding pump 13 Control part 14 Turbidity sensor 15 Optical sensor 151 Light projection part 152 Light reception 153 Reflector 16 Weight sensor 17 Display / Operation unit 20 Centrifuge 21 Blood collection / return line 21a Blood collection needle side line 21b Centrifuge side first line 21c Branch connector 21d Chamber 21f Branch connector 21g Pump tube 21h Tube 21i Filter 22 Collection bag side line 22b Branch connector 22c Branch connector 22d Branch connector 22e Branch connector 22f Filter 22g Branch connector 22h Filter 23 Anticoagulant injection line 23a Pump tube 23b Disinfection filter 2 3c Bubble removal chamber 23d Anticoagulant container connection needle 25 Plasma collection bag 26 Platelet collection bag 261 Leukocyte removal filter 27a Intermediate bag 27b Air bag 28 Bag 29 Blood collection needle 31-36 Bubble sensor 41-51 Tube 61 Bag 70 Cassette housing 81-87 First to seventh flow path opening / closing means 131 Plasma layer 132 Buffy coat layer 133 Red blood cell layer 141 Tubular body 142 Rotor 143 Inlet port 144 Outlet port 145 Upper portion 146 Blood storage space 147 Reflecting surface 201 Housing 202 Leg portion 203 Motor 204 Rotating shaft 205 Fixed base 206 Bolt 207 Spacer 301 to 303 Hanger S001 to S013, S101 to S124 Step

Claims (9)

A blood component collection device that centrifuges blood collected from a donor, collects a predetermined blood component, and returns the remaining blood component to the donor,
Blood collection means for collecting blood from a donor,
A centrifuge for centrifuging blood collected by the blood collection means;
A blood component collection bag for collecting a predetermined blood component separated by the centrifuge;
A blood collection / return line connecting the blood collection means and the centrifuge;
A blood component collection circuit having an anticoagulant injection line having one end connected in the middle of the blood collection / return line;
An anticoagulant feeding pump for feeding the anticoagulant of the anticoagulant injection line;
A first detection means attached to the blood collection / return line and detecting gas and liquid in the blood collection / return line;
A control unit for controlling the operation of the anticoagulant feeding pump,
The controller controls the anticoagulation until the anticoagulant is detected by the first detection means when the anticoagulant is fed from the other end of the anticoagulant injection line prior to the start of blood collection. A blood component collecting apparatus, wherein it is determined whether or not the blood collection / return line is attached to the first detection means based on an operation amount of a drug delivery pump.   The control unit measures the amount of the anticoagulant delivered based on the operation amount of the anticoagulant delivery pump, and before the amount of the anticoagulant delivered reaches a first predetermined amount. The blood component collection according to claim 1, wherein when the anticoagulant is detected by the first detection means, it is determined that the anticoagulant injection line is erroneously attached to the first detection means. apparatus.   The blood component according to claim 2, wherein when the control unit determines that the anticoagulant injection line is erroneously attached to the first detection means, the control unit stops the operation of the anticoagulant feeding pump. Collection device.   The controller measures the amount of the anticoagulant delivered based on the operating amount of the anticoagulant delivery pump, and the amount of the anticoagulant delivered reaches a first predetermined amount. When the anticoagulant is not detected by the first detection means, the anticoagulant liquid is continued to be fed, and the anticoagulant liquid feed amount is larger than the first predetermined amount. 2. When the anticoagulant is detected by the first detection means before the predetermined amount of 2 is reached, it is determined that the blood collection / return line is attached to the first detection means. The blood component collection device according to any one of 1 to 3.   If the anticoagulant is not detected by the first detection means even when the amount of the anticoagulant delivered reaches the second predetermined amount, the control unit collects and returns blood. The blood component collection apparatus according to claim 4, wherein it is determined that a line is not attached to the first detection means. Furthermore, the first detection means attached to the blood collection / return line is attached to the centrifugal separator side, and second detection means for detecting gas and liquid in the blood collection / return line is provided. ,
The control unit measures the amount of the anticoagulant delivered based on the operation amount of the anticoagulant delivery pump, and before the amount of the anticoagulant delivered reaches a first predetermined amount. When the anticoagulant is detected by the first detection unit or the second detection unit, the anticoagulant injection line is erroneously attached to the first detection unit and / or the second detection unit. The blood component collection device according to any one of claims 1 to 5, wherein the blood component collection device determines that the blood component is present. And a third detection means mounted on the anticoagulant injection line for detecting gas and liquid in the anticoagulant injection line.
The control unit measures the liquid feeding amount of the anticoagulant based on the operation amount of the anticoagulant liquid feeding pump, detects the anticoagulant by the third detection means, and then performs the first operation. Whether the blood collection / return line is attached to the first detection means is determined according to the amount of the anticoagulant delivered until the detection means detects the anticoagulant. The blood component collection device according to any one of the above.   The controller is configured so that a liquid feeding amount of the anticoagulant from the detection of the anticoagulant by the third detection unit to the detection of the anticoagulant by the first detection unit is a first place. 8. When the anticoagulant is detected by the first detection means before reaching a fixed amount, it is determined that the anticoagulant injection line is erroneously attached to the first detection means. The blood component collection device described in 1. Furthermore, the second detection means mounted on the centrifugal separator side than the first detection means mounted on the blood collection / return line, and detects gas and liquid in the blood collection / return line;
A third detection means mounted on the anticoagulant injection line and detecting gas and liquid in the anticoagulant injection line;
The control unit measures the liquid feeding amount of the anticoagulant based on the operation amount of the anticoagulant liquid feeding pump, detects the anticoagulant by the third detection means, and then performs the first operation. Before the detection of the anticoagulant by the detection means reaches the first predetermined amount, the anticoagulant by the first detection means or the second detection means. 6. When detecting the above, it is determined that the anticoagulant injection line is erroneously attached to the first detection means and / or the second detection means. Blood component collection device.

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