JPWO2006004214A1 - リン酸化タンパク質のプロテオーム解析方法 - Google Patents

リン酸化タンパク質のプロテオーム解析方法 Download PDF

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JPWO2006004214A1
JPWO2006004214A1 JP2006529017A JP2006529017A JPWO2006004214A1 JP WO2006004214 A1 JPWO2006004214 A1 JP WO2006004214A1 JP 2006529017 A JP2006529017 A JP 2006529017A JP 2006529017 A JP2006529017 A JP 2006529017A JP WO2006004214 A1 JPWO2006004214 A1 JP WO2006004214A1
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protein
sample
phosphorylated
proteins
database
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JP2006529017A
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English (en)
Japanese (ja)
Inventor
吉哉 小田
吉哉 小田
田畑 剛
剛 田畑
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Eisai R&D Management Co Ltd
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Eisai R&D Management Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/86Signal analysis
    • G01N30/8675Evaluation, i.e. decoding of the signal into analytical information
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00Aspects relating to sorbent materials
    • B01J2220/50Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54Sorbents specially adapted for analytical or investigative chromatography

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Library & Information Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
JP2006529017A 2004-07-02 2005-07-04 リン酸化タンパク質のプロテオーム解析方法 Pending JPWO2006004214A1 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2004197344 2004-07-02
JP2004197344 2004-07-02
PCT/JP2005/012714 WO2006004214A1 (ja) 2004-07-02 2005-07-04 リン酸化タンパク質のプロテオーム解析方法

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP2010017929A Division JP2010133971A (ja) 2004-07-02 2010-01-29 リン酸化タンパク質のプロテオーム解析方法
JP2010291592A Division JP2011090012A (ja) 2004-07-02 2010-12-28 リン酸化タンパク質のプロテオーム解析方法

Publications (1)

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JPWO2006004214A1 true JPWO2006004214A1 (ja) 2008-04-24

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JP2006529017A Pending JPWO2006004214A1 (ja) 2004-07-02 2005-07-04 リン酸化タンパク質のプロテオーム解析方法
JP2010017929A Withdrawn JP2010133971A (ja) 2004-07-02 2010-01-29 リン酸化タンパク質のプロテオーム解析方法
JP2010291592A Withdrawn JP2011090012A (ja) 2004-07-02 2010-12-28 リン酸化タンパク質のプロテオーム解析方法

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JP2010017929A Withdrawn JP2010133971A (ja) 2004-07-02 2010-01-29 リン酸化タンパク質のプロテオーム解析方法
JP2010291592A Withdrawn JP2011090012A (ja) 2004-07-02 2010-12-28 リン酸化タンパク質のプロテオーム解析方法

Country Status (5)

Country Link
US (1) US8131479B2 (https=)
EP (2) EP2113562B1 (https=)
JP (3) JPWO2006004214A1 (https=)
AT (2) ATE545460T1 (https=)
WO (1) WO2006004214A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012524252A (ja) * 2009-04-17 2012-10-11 クイーン マリー アンド ウエストフィールド カレッジ 修飾ペプチドの定量化方法

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100855620B1 (ko) 2005-12-13 2008-09-03 주식회사 프로바이온 메조다공성 이산화티탄을 이용한 분리 또는 농축방법
WO2008148645A1 (en) * 2007-06-07 2008-12-11 Syddansk Universitet Separation of mono- from multi-phoshorylated peptides
WO2009102258A1 (en) * 2008-02-15 2009-08-20 Ge Healthcare Bio-Sciences Ab Separation of biomolecules
JP5181908B2 (ja) * 2008-08-04 2013-04-10 株式会社島津製作所 質量分析データ解析装置
EP2588428A4 (en) * 2010-06-30 2014-03-05 Univ Saskatchewan Kinome ANALYSIS PROCEDURE
GB201017721D0 (en) * 2010-10-20 2010-12-01 Queen Mary & Westfield College Method
US9659146B2 (en) 2011-05-02 2017-05-23 Tyler Stuart Bray Method for quantitative analysis of complex proteomic data
JP5776443B2 (ja) * 2011-08-29 2015-09-09 株式会社島津製作所 質量分析を用いた修飾タンパク質同定方法及び同定装置
GB201204278D0 (en) 2012-03-09 2012-04-25 Queen Mary & Westfield College Method
GB201520178D0 (en) 2015-11-16 2015-12-30 Univ London Queen Mary Method
CN108287200B (zh) * 2017-04-24 2020-12-18 麦特绘谱生物科技(上海)有限公司 质谱参照数据库的建立方法及基于其的物质分析方法
JP6928816B2 (ja) * 2017-12-14 2021-09-01 株式会社Lsiメディエンス 質量分析計を用いたタンパク質分析のためのタンパク質含有試料の前処理方法
CN108872442B (zh) * 2018-08-17 2021-04-27 无限极(中国)有限公司 一种基于谱效关系的骨胶原肽质量检测方法
AU2020288652A1 (en) * 2019-06-05 2021-11-18 Amgen Inc. Methods of identifying attributes of therapeutic proteins

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002072863A2 (en) * 2001-03-09 2002-09-19 Applera Corporation Methods for large scale protein matching
JP2003521257A (ja) * 2000-02-07 2003-07-15 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ (ポリ)ペプチドを同定しおよび/または特性決定するための方法
WO2003065031A1 (en) * 2002-01-31 2003-08-07 Gl Sciences Incorporated Method and apparatus for analyzing amino acid, peptide, protein, saccharide or lipid

Family Cites Families (3)

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US5674987A (en) 1994-07-22 1997-10-07 Anatrace, Inc. Extraction of proteins from naturally occurring membranes
US7063966B2 (en) 2001-07-11 2006-06-20 Sri International Chimeric G protein coupled receptors
US7026167B2 (en) * 2001-12-28 2006-04-11 University Of Virginia Patent Foundation Systems and methods for the analysis of protein phosphorylation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003521257A (ja) * 2000-02-07 2003-07-15 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ (ポリ)ペプチドを同定しおよび/または特性決定するための方法
WO2002072863A2 (en) * 2001-03-09 2002-09-19 Applera Corporation Methods for large scale protein matching
WO2003065031A1 (en) * 2002-01-31 2003-08-07 Gl Sciences Incorporated Method and apparatus for analyzing amino acid, peptide, protein, saccharide or lipid

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JPN6009061312, 小田吉哉, ""リン酸化プロテオーム解析の最新技術"", 実験医学, 2002, Vol.20, No.1, p.23−27 *
JPN6009061315, 平野久,外1名, ""タンパク質の翻訳後修飾の解析"", 実験医学, 2002, Vol.20, No.14, p.45−50 *
JPN6009061318, 小田吉哉, ""タンパク質の翻訳後修飾の解析"", 現代化学 増刊42 プロテオミクス−方法とその病態解析への応用−, 2002, p.24−30 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012524252A (ja) * 2009-04-17 2012-10-11 クイーン マリー アンド ウエストフィールド カレッジ 修飾ペプチドの定量化方法

Also Published As

Publication number Publication date
EP2113562B1 (en) 2012-02-15
EP1780537A4 (en) 2009-03-25
JP2010133971A (ja) 2010-06-17
WO2006004214A1 (ja) 2006-01-12
EP2113562A1 (en) 2009-11-04
JP2011090012A (ja) 2011-05-06
US20080221802A1 (en) 2008-09-11
ATE525462T1 (de) 2011-10-15
ATE545460T1 (de) 2012-03-15
US8131479B2 (en) 2012-03-06
EP1780537B1 (en) 2011-09-21
EP1780537A1 (en) 2007-05-02

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