JPS6486899A - Measurement of activity of adenosine deaminase and reagent for measuring said activity - Google Patents
Measurement of activity of adenosine deaminase and reagent for measuring said activityInfo
- Publication number
- JPS6486899A JPS6486899A JP24093087A JP24093087A JPS6486899A JP S6486899 A JPS6486899 A JP S6486899A JP 24093087 A JP24093087 A JP 24093087A JP 24093087 A JP24093087 A JP 24093087A JP S6486899 A JPS6486899 A JP S6486899A
- Authority
- JP
- Japan
- Prior art keywords
- activity
- reaction
- adenosine deaminase
- value
- absorbance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE:To measure the titled activity for diagnosing hepatic diseases by one-stage enzymatic reaction simply and in high precision, by enzymatically reacting a specimen adenosine deaminase with 2-amino-adenosine and measuring values of absorbance at a specific wavelength before and after the reaction. CONSTITUTION:A specimen containing adenosine deaminase is enzymatically reacted with 0.05M phosphoric acid buffer solution (pH 7.2) containing 2-amino- adenosine at 37 deg.C for 10min, the enzymatic reaction is stopped by adding 1N phosphoric acid solution, values of absorbance of the mixed solution at 280-300nm before and after the reaction are measured, the value obtained by taking away the absorbance value before the reaction from the absorbance value after the reaction is calculated and the value is read from a calibration curve to obtain the activity value of adenosine deaminase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24093087A JPS6486899A (en) | 1987-09-28 | 1987-09-28 | Measurement of activity of adenosine deaminase and reagent for measuring said activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24093087A JPS6486899A (en) | 1987-09-28 | 1987-09-28 | Measurement of activity of adenosine deaminase and reagent for measuring said activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6486899A true JPS6486899A (en) | 1989-03-31 |
Family
ID=17066757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24093087A Pending JPS6486899A (en) | 1987-09-28 | 1987-09-28 | Measurement of activity of adenosine deaminase and reagent for measuring said activity |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6486899A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011086740A1 (en) | 2010-01-15 | 2011-07-21 | コミー株式会社 | Mirror for checking blind spot |
-
1987
- 1987-09-28 JP JP24093087A patent/JPS6486899A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011086740A1 (en) | 2010-01-15 | 2011-07-21 | コミー株式会社 | Mirror for checking blind spot |
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