JPS6486871A - Method for separating and purifying recombinant protein - Google Patents
Method for separating and purifying recombinant proteinInfo
- Publication number
- JPS6486871A JPS6486871A JP24701787A JP24701787A JPS6486871A JP S6486871 A JPS6486871 A JP S6486871A JP 24701787 A JP24701787 A JP 24701787A JP 24701787 A JP24701787 A JP 24701787A JP S6486871 A JPS6486871 A JP S6486871A
- Authority
- JP
- Japan
- Prior art keywords
- microbial cells
- chromatography
- escherichia coli
- bacillus subtilis
- recombinant plasmid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 230000000813 microbial effect Effects 0.000 abstract 3
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 2
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 2
- 239000013612 plasmid Substances 0.000 abstract 2
- 229960002385 streptomycin sulfate Drugs 0.000 abstract 2
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 101900343733 Bacillus subtilis Dihydrofolate reductase Proteins 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000004440 column chromatography Methods 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 238000001641 gel filtration chromatography Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24701787A JPS6486871A (en) | 1987-09-30 | 1987-09-30 | Method for separating and purifying recombinant protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24701787A JPS6486871A (en) | 1987-09-30 | 1987-09-30 | Method for separating and purifying recombinant protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6486871A true JPS6486871A (en) | 1989-03-31 |
| JPH0333315B2 JPH0333315B2 (enrdf_load_stackoverflow) | 1991-05-16 |
Family
ID=17157159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24701787A Granted JPS6486871A (en) | 1987-09-30 | 1987-09-30 | Method for separating and purifying recombinant protein |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6486871A (enrdf_load_stackoverflow) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6953991B2 (en) | 2000-07-19 | 2005-10-11 | Shindo Company, Ltd. | Semiconductor device |
-
1987
- 1987-09-30 JP JP24701787A patent/JPS6486871A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0333315B2 (enrdf_load_stackoverflow) | 1991-05-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IE883612L (en) | Vectors and compounds for direct expression of activated human protein c | |
| Sideras et al. | Partial biochemical characterization of IgG1‐inducing factor | |
| ES2046177T3 (es) | Construccion de una proteina enlazante a igg para facilitar el tratamiento aguas abajo utilizando ingenieria de proteinas. | |
| EP0445646A3 (en) | Dna fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the dna fragment and a process for the production of amides using the transformant | |
| AU4251989A (en) | Method for the purification of proteins | |
| EP0131142A3 (en) | Process for recovering a cell wall protein | |
| AU1049788A (en) | Methods and peptides for the treatment of non-ige-mediated diseases | |
| BR8301918A (pt) | Proteina,sequencia de dna,veiculo de expressao replicavel,microorganismo ou cultura celular,plasmideo,composicao,cultura de celulas,utilizacao de tal proteina,processo de expressao,processo para producao de um veiculo de expressao e processo para preparacao de uma proteina uroquinase | |
| CA1267497C (en) | PROTEIN RECOVERY | |
| SE8402861D0 (sv) | Rening av biologiskt material | |
| IL80960A0 (en) | Process for improving the expression of eukaryotic proteins | |
| EP0263518A3 (en) | Purification of recombinant tumor necrosis factor | |
| EP0269455A3 (en) | Highly purified fused protein comprising human ige fc fragment and production thereof | |
| AU551490B2 (en) | Dna code for protein to suppress abnormal protein degradation | |
| JPS6486871A (en) | Method for separating and purifying recombinant protein | |
| DK0497828T4 (da) | Fremgangsmåde til endoproteolytisk behandling af (forstadie)proteiner og til (mikro)biologisk produktion af proteiner. | |
| EP0159943A3 (en) | Expression vectors, dna fragments, peptides, hosts, and process for production of proteins | |
| Rappaport et al. | Non phosphorylation in vitro of the 6 S tubulin from brain and thyroid tissue | |
| Nakata et al. | Simple and rapid purification of tryptophan 5-monooxygenase from rabbit brain by affinity chromatography | |
| Hillson et al. | Bovine liver thiol-protein disulphide oxidoreductases. An alternative method for differential purification and resolution of protein disulphide-isomerase and glutathione-insulin transhydrogenase | |
| RU2136749C1 (ru) | Способ получения эндонуклеазы рестрикции hinf i | |
| KR970001813B1 (ko) | 삼상분할법을 이용한 아미노펩티다제의 정제방법 | |
| AU590712B2 (en) | Immune interferon and method for its purification | |
| ATE154074T1 (de) | Verfahren zur herstellung und reinigung des carboxyterminalen fragments der dna-polymerase i von streptococcus pneumoniae | |
| Kumar et al. | Rapid isolation of lysyl-tRNA synthetase from rat liver |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |