JPS6474981A - Method for cultivating plant tissue and apparatus therefor - Google Patents
Method for cultivating plant tissue and apparatus thereforInfo
- Publication number
- JPS6474981A JPS6474981A JP62230490A JP23049087A JPS6474981A JP S6474981 A JPS6474981 A JP S6474981A JP 62230490 A JP62230490 A JP 62230490A JP 23049087 A JP23049087 A JP 23049087A JP S6474981 A JPS6474981 A JP S6474981A
- Authority
- JP
- Japan
- Prior art keywords
- calluses
- culture
- culture fluid
- adsorption
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE:To store an useful substance of plant bodies in a liquid, remove only noxious browned substances and enable cultivation without damaging the plant bodies, by using an adsorbent attaining adsorption equilibrium with a culture fluid. CONSTITUTION:Active carbon is filled in an adsorption column 15 having 20mm inside diameter and 65mm length and an MS culture medium is fed thereto at 5ml/min flow rate for 12hr to provide the action carbon in an adsorption equilibrium state. Calluses 2 of the genus Cymbidium are then placed in a culture column 11 equipped with a perforated plate having 60mm inside diameter and 150mm length and an MS culture medium is passed through the adsorption column 15 for a given time and then sprayed on the calluses. The culture fluid is subsequently circulated through this loop and continuous operation is carried out. An illuminator 19 is lit for 12hr and put out for 12hr and temperature is kept at 23 deg.C to carry out cultivation for about 90 days. Browning of the culture fluid is not observed and green calluses are propagated.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62230490A JPS6474981A (en) | 1987-09-14 | 1987-09-14 | Method for cultivating plant tissue and apparatus therefor |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62230490A JPS6474981A (en) | 1987-09-14 | 1987-09-14 | Method for cultivating plant tissue and apparatus therefor |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6474981A true JPS6474981A (en) | 1989-03-20 |
Family
ID=16908592
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62230490A Pending JPS6474981A (en) | 1987-09-14 | 1987-09-14 | Method for cultivating plant tissue and apparatus therefor |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6474981A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000068357A1 (en) * | 1999-05-06 | 2000-11-16 | Universite Laval | Scalable bioreactor culture process and system for the maturation of conifer somatic embryos |
-
1987
- 1987-09-14 JP JP62230490A patent/JPS6474981A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000068357A1 (en) * | 1999-05-06 | 2000-11-16 | Universite Laval | Scalable bioreactor culture process and system for the maturation of conifer somatic embryos |
US7235402B2 (en) | 1999-05-06 | 2007-06-26 | Universite Laval | Scalable bioreactor culture process and system for the maturation of conifer somatic embryos |
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