JPS6451090A - Monoclonal antibody, production thereof and enzyme determining reagent comprising said antibody - Google Patents

Monoclonal antibody, production thereof and enzyme determining reagent comprising said antibody

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Publication number
JPS6451090A
JPS6451090A JP62205073A JP20507387A JPS6451090A JP S6451090 A JPS6451090 A JP S6451090A JP 62205073 A JP62205073 A JP 62205073A JP 20507387 A JP20507387 A JP 20507387A JP S6451090 A JPS6451090 A JP S6451090A
Authority
JP
Japan
Prior art keywords
antibody
mouse
cell
monoclonal antibody
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62205073A
Other languages
Japanese (ja)
Other versions
JP2650155B2 (en
Inventor
Hiroshi Sakai
Tsugukazu Komoda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nisshin Seifun Group Inc
Original Assignee
Nisshin Seifun Group Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nisshin Seifun Group Inc filed Critical Nisshin Seifun Group Inc
Priority to JP62205073A priority Critical patent/JP2650155B2/en
Publication of JPS6451090A publication Critical patent/JPS6451090A/en
Application granted granted Critical
Publication of JP2650155B2 publication Critical patent/JP2650155B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE:To obtain a monoclonal antibody useful for diagnosis of heart diseases and hepatic diseases, by preparing an antibody-forming cell from a mouse immunized against human LDHM subunit and cloning the antibody-forming cell. CONSTITUTION:A mouse is immunized against an isozyme of human LDH (lactic dehydrogenase) V type, a splenocyte is collected from the mouse, fused to a mouse myeloma cell, a cell group to produce an antibody is selected by ELISA and cloned to give fused cell LD-10, 11, 17, 21, 25, 52, 58 and 66 strains to produce a monoclonal specific to human LDHM subunit. The strain is cultivated in a medium or transplanted in abdominal cavity of a mouse, made into ascites tumor and the aimed monoclonal antibody is obtained from the culture solution or ascites.
JP62205073A 1987-08-20 1987-08-20 Monoclonal antibodies, their preparation and reagents for enzyme determination Expired - Fee Related JP2650155B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62205073A JP2650155B2 (en) 1987-08-20 1987-08-20 Monoclonal antibodies, their preparation and reagents for enzyme determination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62205073A JP2650155B2 (en) 1987-08-20 1987-08-20 Monoclonal antibodies, their preparation and reagents for enzyme determination

Publications (2)

Publication Number Publication Date
JPS6451090A true JPS6451090A (en) 1989-02-27
JP2650155B2 JP2650155B2 (en) 1997-09-03

Family

ID=16500976

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62205073A Expired - Fee Related JP2650155B2 (en) 1987-08-20 1987-08-20 Monoclonal antibodies, their preparation and reagents for enzyme determination

Country Status (1)

Country Link
JP (1) JP2650155B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003047470A (en) * 2001-08-01 2003-02-18 Asahi Kasei Corp New antibody and use thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3623846A1 (en) * 1985-07-17 1987-01-22 Univ Ramot COMPOSITION FOR SELECTIVE DETERMINATION OF A BIOLOGICALLY ACTIVE SUBSTANCE

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3623846A1 (en) * 1985-07-17 1987-01-22 Univ Ramot COMPOSITION FOR SELECTIVE DETERMINATION OF A BIOLOGICALLY ACTIVE SUBSTANCE

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003047470A (en) * 2001-08-01 2003-02-18 Asahi Kasei Corp New antibody and use thereof

Also Published As

Publication number Publication date
JP2650155B2 (en) 1997-09-03

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Legal Events

Date Code Title Description
LAPS Cancellation because of no payment of annual fees