JPS6451090A - Monoclonal antibody, production thereof and enzyme determining reagent comprising said antibody - Google Patents
Monoclonal antibody, production thereof and enzyme determining reagent comprising said antibodyInfo
- Publication number
- JPS6451090A JPS6451090A JP62205073A JP20507387A JPS6451090A JP S6451090 A JPS6451090 A JP S6451090A JP 62205073 A JP62205073 A JP 62205073A JP 20507387 A JP20507387 A JP 20507387A JP S6451090 A JPS6451090 A JP S6451090A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- mouse
- cell
- monoclonal antibody
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE:To obtain a monoclonal antibody useful for diagnosis of heart diseases and hepatic diseases, by preparing an antibody-forming cell from a mouse immunized against human LDHM subunit and cloning the antibody-forming cell. CONSTITUTION:A mouse is immunized against an isozyme of human LDH (lactic dehydrogenase) V type, a splenocyte is collected from the mouse, fused to a mouse myeloma cell, a cell group to produce an antibody is selected by ELISA and cloned to give fused cell LD-10, 11, 17, 21, 25, 52, 58 and 66 strains to produce a monoclonal specific to human LDHM subunit. The strain is cultivated in a medium or transplanted in abdominal cavity of a mouse, made into ascites tumor and the aimed monoclonal antibody is obtained from the culture solution or ascites.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62205073A JP2650155B2 (en) | 1987-08-20 | 1987-08-20 | Monoclonal antibodies, their preparation and reagents for enzyme determination |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62205073A JP2650155B2 (en) | 1987-08-20 | 1987-08-20 | Monoclonal antibodies, their preparation and reagents for enzyme determination |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6451090A true JPS6451090A (en) | 1989-02-27 |
JP2650155B2 JP2650155B2 (en) | 1997-09-03 |
Family
ID=16500976
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62205073A Expired - Fee Related JP2650155B2 (en) | 1987-08-20 | 1987-08-20 | Monoclonal antibodies, their preparation and reagents for enzyme determination |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2650155B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003047470A (en) * | 2001-08-01 | 2003-02-18 | Asahi Kasei Corp | New antibody and use thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3623846A1 (en) * | 1985-07-17 | 1987-01-22 | Univ Ramot | COMPOSITION FOR SELECTIVE DETERMINATION OF A BIOLOGICALLY ACTIVE SUBSTANCE |
-
1987
- 1987-08-20 JP JP62205073A patent/JP2650155B2/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3623846A1 (en) * | 1985-07-17 | 1987-01-22 | Univ Ramot | COMPOSITION FOR SELECTIVE DETERMINATION OF A BIOLOGICALLY ACTIVE SUBSTANCE |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003047470A (en) * | 2001-08-01 | 2003-02-18 | Asahi Kasei Corp | New antibody and use thereof |
Also Published As
Publication number | Publication date |
---|---|
JP2650155B2 (en) | 1997-09-03 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
LAPS | Cancellation because of no payment of annual fees |