JPS64469A - Method and kit for measuring high-molecular hyaluronic acid - Google Patents
Method and kit for measuring high-molecular hyaluronic acidInfo
- Publication number
- JPS64469A JPS64469A JP4922788A JP4922788A JPS64469A JP S64469 A JPS64469 A JP S64469A JP 4922788 A JP4922788 A JP 4922788A JP 4922788 A JP4922788 A JP 4922788A JP S64469 A JPS64469 A JP S64469A
- Authority
- JP
- Japan
- Prior art keywords
- hyaluronic acid
- bondable
- protein
- solid phase
- conjugate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE: To measure hyaluronic acid with high sensitivity by forming the hyaluronic acid bondable protein fixed to a solid phase, specimen hyaluronic acid and labeled hyaluronic acid bondable protein to a sandwich-like conjugate.
CONSTITUTION: The hyaluronic acid bondable protein is first fixed to the surface of the solid phase. A sample contg. the specimen hyaluronic acid is then added to the hyaluronic acid bondable protein fixed to the solid phase to bond the high-molecular hyaluronic acid. The hyaluronic acid bondable protein previously labeled with a labeling material is added further to the above-mentioned solid phase. The sandwich-like conjugate of the hyaluronic acid bondable protein fixed to the solid phase and the labeled hyaluronic acid bondable protein is thereby formed. This sandwich-like conjugate sandwiches the high-molecular hyaluronic acid to be measured and the low-molecular hyaluronic acid which is not the object to be measured is not formed in the above-mentioned conjugate. Since the labeling material is detected, the high-molecular hyaluronic acid is selectively detectable and inflammations such as rheumatism are effectively diagnosable.
COPYRIGHT: (C)1989,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP63049227A JPH0641952B2 (en) | 1987-03-03 | 1988-03-02 | Method and kit for measuring high molecular hyaluronic acid |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4851887 | 1987-03-03 | ||
JP62-48518 | 1987-03-03 | ||
JP63049227A JPH0641952B2 (en) | 1987-03-03 | 1988-03-02 | Method and kit for measuring high molecular hyaluronic acid |
Publications (3)
Publication Number | Publication Date |
---|---|
JPH01469A JPH01469A (en) | 1989-01-05 |
JPS64469A true JPS64469A (en) | 1989-01-05 |
JPH0641952B2 JPH0641952B2 (en) | 1994-06-01 |
Family
ID=26388806
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP63049227A Expired - Lifetime JPH0641952B2 (en) | 1987-03-03 | 1988-03-02 | Method and kit for measuring high molecular hyaluronic acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0641952B2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000012122A3 (en) * | 1998-08-27 | 2000-06-22 | Universitaetsklinikum Freiburg | Low-molecular fragments of hyaluronic acid for the preparation of vaccines |
JP5466405B2 (en) * | 2006-08-08 | 2014-04-09 | 生化学工業株式会社 | Method for measuring the molecular weight of hyaluronic acid |
CN117664885A (en) * | 2023-11-28 | 2024-03-08 | 中国计量科学研究院 | Quantitative detection method for content of hyaluronic acid in fabric |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5347519A (en) * | 1976-10-07 | 1978-04-28 | Mochida Pharm Co Ltd | Immunologically measuring method |
US4244940A (en) * | 1978-09-05 | 1981-01-13 | Bio-Rad Laboratories, Inc. | Single-incubation two-site immunoassay |
-
1988
- 1988-03-02 JP JP63049227A patent/JPH0641952B2/en not_active Expired - Lifetime
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5347519A (en) * | 1976-10-07 | 1978-04-28 | Mochida Pharm Co Ltd | Immunologically measuring method |
US4244940A (en) * | 1978-09-05 | 1981-01-13 | Bio-Rad Laboratories, Inc. | Single-incubation two-site immunoassay |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000012122A3 (en) * | 1998-08-27 | 2000-06-22 | Universitaetsklinikum Freiburg | Low-molecular fragments of hyaluronic acid for the preparation of vaccines |
US6838086B1 (en) | 1998-08-27 | 2005-01-04 | Universitaetsklinikum Freiburg | Composition comprising low molecular weight hyaluronic acid fragments |
JP5466405B2 (en) * | 2006-08-08 | 2014-04-09 | 生化学工業株式会社 | Method for measuring the molecular weight of hyaluronic acid |
CN117664885A (en) * | 2023-11-28 | 2024-03-08 | 中国计量科学研究院 | Quantitative detection method for content of hyaluronic acid in fabric |
Also Published As
Publication number | Publication date |
---|---|
JPH0641952B2 (en) | 1994-06-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
EXPY | Cancellation because of completion of term | ||
FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20080601 Year of fee payment: 14 |