JPS644148B2 - - Google Patents
Info
- Publication number
- JPS644148B2 JPS644148B2 JP15758682A JP15758682A JPS644148B2 JP S644148 B2 JPS644148 B2 JP S644148B2 JP 15758682 A JP15758682 A JP 15758682A JP 15758682 A JP15758682 A JP 15758682A JP S644148 B2 JPS644148 B2 JP S644148B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- paraffin
- staining
- electron microscope
- pretreatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- -1 fatty acid ester Chemical class 0.000 claims description 10
- 239000012188 paraffin wax Substances 0.000 claims description 10
- 238000010186 staining Methods 0.000 claims description 7
- 235000019441 ethanol Nutrition 0.000 claims description 6
- 229920001214 Polysorbate 60 Polymers 0.000 claims description 5
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 5
- 229930195729 fatty acid Natural products 0.000 claims description 5
- 239000000194 fatty acid Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 229920000136 polysorbate Polymers 0.000 description 4
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000001493 electron microscopy Methods 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000004626 scanning electron microscopy Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15758682A JPS5946534A (ja) | 1982-09-09 | 1982-09-09 | 試料の染色前処理方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15758682A JPS5946534A (ja) | 1982-09-09 | 1982-09-09 | 試料の染色前処理方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5946534A JPS5946534A (ja) | 1984-03-15 |
| JPS644148B2 true JPS644148B2 (enExample) | 1989-01-24 |
Family
ID=15652935
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15758682A Granted JPS5946534A (ja) | 1982-09-09 | 1982-09-09 | 試料の染色前処理方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5946534A (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0653617A3 (en) * | 1993-11-12 | 1997-10-22 | Shino Junko | Fat-soluble substitute for the substitution of a drainage agent for the manufacture of a tissue preparation. |
| JP4946047B2 (ja) * | 2005-12-28 | 2012-06-06 | 東洋紡績株式会社 | 有形成分分類装置用粒子標準試薬 |
| CN105241685B (zh) * | 2015-08-10 | 2018-04-13 | 河南科技大学 | 小鲵科动物皮肤显微组织切片的制备方法 |
-
1982
- 1982-09-09 JP JP15758682A patent/JPS5946534A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5946534A (ja) | 1984-03-15 |
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