JPS6439983A - Microbead for microcarrier cell culture - Google Patents

Microbead for microcarrier cell culture

Info

Publication number
JPS6439983A
JPS6439983A JP62195342A JP19534287A JPS6439983A JP S6439983 A JPS6439983 A JP S6439983A JP 62195342 A JP62195342 A JP 62195342A JP 19534287 A JP19534287 A JP 19534287A JP S6439983 A JPS6439983 A JP S6439983A
Authority
JP
Japan
Prior art keywords
microbeads
medium
cell culture
polymer
magnet
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP62195342A
Other languages
Japanese (ja)
Other versions
JPH0443636B2 (en
Inventor
Shuji Sakuma
Kiminori Atsumi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sangi Co Ltd
Original Assignee
Sangi Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sangi Co Ltd filed Critical Sangi Co Ltd
Priority to JP62195342A priority Critical patent/JPS6439983A/en
Publication of JPS6439983A publication Critical patent/JPS6439983A/en
Publication of JPH0443636B2 publication Critical patent/JPH0443636B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To obtain the titled microbeads readily changing a medium, enabling high-density cell culture, by adding a magnetic material to the microbeads. CONSTITUTION:Polymer powder selected from styrol polymer, ABS polymer, PE and polyamide polymer is uniformly blended with magnetic material (e.g. iron powder) having <=10mu particle diameter, heated, melted and dry sprayed from an orifice having a given diameter to give microbeads which are useful for microcarrier cell culture, apply magnetic force to fine particles and have 50-200mu particle diameter and 1.16-1.04 specific gravity. Then a magnet is exerted on the microbeads from the outside, the microbeads are aggregated in the circumference of the magnet, the microbeads are separated from a medium, the medium is taken out from the system, a new medium is added, magnetic force is cut and culture is continued.
JP62195342A 1987-08-06 1987-08-06 Microbead for microcarrier cell culture Granted JPS6439983A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62195342A JPS6439983A (en) 1987-08-06 1987-08-06 Microbead for microcarrier cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62195342A JPS6439983A (en) 1987-08-06 1987-08-06 Microbead for microcarrier cell culture

Publications (2)

Publication Number Publication Date
JPS6439983A true JPS6439983A (en) 1989-02-10
JPH0443636B2 JPH0443636B2 (en) 1992-07-17

Family

ID=16339573

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62195342A Granted JPS6439983A (en) 1987-08-06 1987-08-06 Microbead for microcarrier cell culture

Country Status (1)

Country Link
JP (1) JPS6439983A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012029877A1 (en) * 2010-08-31 2012-03-08 株式会社セルシード Temperature-responsive cell culture beads and method for manufacturing same
JP2013172662A (en) * 2012-02-24 2013-09-05 Kanazawa Inst Of Technology Substance conversion method, method for producing bioreactor and bioreactor

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4833088A (en) * 1971-08-27 1973-05-07
JPS4833066A (en) * 1971-09-02 1973-05-07

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4833088A (en) * 1971-08-27 1973-05-07
JPS4833066A (en) * 1971-09-02 1973-05-07

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012029877A1 (en) * 2010-08-31 2012-03-08 株式会社セルシード Temperature-responsive cell culture beads and method for manufacturing same
JPWO2012029877A1 (en) * 2010-08-31 2013-10-31 株式会社セルシード Temperature-responsive cell culture beads and method for producing the same
JP2017012172A (en) * 2010-08-31 2017-01-19 株式会社セルシード Beads for temperature-responsive cell cultures and production method thereof
JP2013172662A (en) * 2012-02-24 2013-09-05 Kanazawa Inst Of Technology Substance conversion method, method for producing bioreactor and bioreactor

Also Published As

Publication number Publication date
JPH0443636B2 (en) 1992-07-17

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