JPS6434286A - Production of luciferase - Google Patents
Production of luciferaseInfo
- Publication number
- JPS6434286A JPS6434286A JP18772487A JP18772487A JPS6434286A JP S6434286 A JPS6434286 A JP S6434286A JP 18772487 A JP18772487 A JP 18772487A JP 18772487 A JP18772487 A JP 18772487A JP S6434286 A JPS6434286 A JP S6434286A
- Authority
- JP
- Japan
- Prior art keywords
- afford
- dna
- luciferase
- luc
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE:To efficiently obtain luciferase useful as an enzyme for determining adenosine triphosphate (ATP), etc., in a short time, by cultivating a specific microorganism in a culture medium. CONSTITUTION:A DNA capable of coding luciferase (LUC) derived from a Photinus.pyralis which is a species of firefly and c-DNA derived from Luciola.cruciata which is 'GENJI BOTARU' are hybridized to provide a plasmid DNA (A) containing the c-DNA capable of coding LUC of about 2.0 kb. The resultant component (A) is then treated with a restriction enzyme to afford a DNA, which is subsequently integrated into a plasmid pUC18 to afford a recombinant DAN (B). The obtained component (B) is then introduced into Escherichia coli JM101 of the genus Escherichia, etc., to provide a microorganism (C) having the ability to produce LUC. The resultant strain (C) is subsequently cultivated in a culture medium containing trypton, etc., at 30-40 deg.C for 4-8hr and centrifuged to afford microbial cells, which are crushed to provide a crude enzyme liquid (D). The obtained liquid (D) is then subjected to purification treatment, such as fractionation with sodium sulfate or gel filtration, to afford the aimed purified luciferase.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18772487A JPS6434286A (en) | 1987-07-29 | 1987-07-29 | Production of luciferase |
US07/224,445 US4968613A (en) | 1987-07-29 | 1988-07-26 | Luciferase gene and novel recombinant DNA as well as a method of producing luciferase |
EP88112233A EP0301541B1 (en) | 1987-07-29 | 1988-07-28 | Luciferase gene and novel recombinant DNA as well as a method of producing luciferase |
DE3887223T DE3887223T2 (en) | 1987-07-29 | 1988-07-28 | Luciferase gene and new recombinant DNA and a process for the production of luciferase. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP18772487A JPS6434286A (en) | 1987-07-29 | 1987-07-29 | Production of luciferase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6434286A true JPS6434286A (en) | 1989-02-03 |
Family
ID=16211070
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP18772487A Pending JPS6434286A (en) | 1987-07-29 | 1987-07-29 | Production of luciferase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6434286A (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6143998A (en) * | 1984-06-05 | 1986-03-03 | アマーシャム インターナショナル パブリック リミティド カンパニー | Detection of bacteria in environment |
-
1987
- 1987-07-29 JP JP18772487A patent/JPS6434286A/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6143998A (en) * | 1984-06-05 | 1986-03-03 | アマーシャム インターナショナル パブリック リミティド カンパニー | Detection of bacteria in environment |
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